CN85102818A - The novel technology for extracting of cianidanol - Google Patents
The novel technology for extracting of cianidanol Download PDFInfo
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- CN85102818A CN85102818A CN 85102818 CN85102818A CN85102818A CN 85102818 A CN85102818 A CN 85102818A CN 85102818 CN85102818 CN 85102818 CN 85102818 A CN85102818 A CN 85102818A CN 85102818 A CN85102818 A CN 85102818A
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- cianidanol
- tannic acid
- protein
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Abstract
The present invention is a technology of extracting cianidanol from the Chinese medicine CATECHU.The process using vegetable-protein combines the mode that generates catechu-tannic acid protein precipitation with tannic acid, remove the crude product that tannic acid obtains cianidanol, uses the plumbic acetate precipitator method or macroreticular resin absorbing method wash-out again, makes the cianidanol elaboration.
Description
One is applicable to from plant, is particularly useful for extracting from Chinese medicine CATECHU (Uncaviagamb) extraction process of cianidanol.
Cianidanol (+)-Catcnin is a flavanols compounds, chemistry is by name 3 ', 4 ', 5,7-tetrahydroxy flavan-3-alcohol, chemical structural formula is:
Cianidanol often is present in some natural phant, can extract with different chemical processes to obtain.
A large amount of pharmacology, biochemistry, clinical study prove that cianidanol (medicine is called Catergen) can promote hepatic energy metabolism in the animal liver poisoning model, stabilizing cell membrane, protection liver organization structure.Can reduce serum bilirubin quickly to human acute hepatitis, shorten stage of icterus.To B-mode and viral hepatitis non-A non-B, can obviously improve liver biochemistry function and histology and change.
Have in the plant of the value extracted at some, especially in the Chinese medicine CATECHU, often exist a large amount of tannic acids, be difficult for removing, brought very big difficulty for the extraction separation cianidanol.Taking reasonable method, remove tannic acid effectively, is the key of extracting cianidanol.
Generally be that the employing ethyl acetate is that solvent extracts from raw material in the prior art, extract cianidanol with the method for gelatin repeated precipitation tannic acid again, owing to used a large amount of ethyl acetate and gelatin in the technology, complex process, exist problems such as three-waste pollution, cost costliness, brought many difficulties to suitability for industrialized production.
Task of the present invention is the technology that an economical and practical extraction cianidanol is provided in order to overcome the deficiencies in the prior art.
The present invention finishes as follows:
Water is that solvent extracts from raw material, can generate the sedimentary characteristic of water-fast tannin albuminate with protein bound according to tannic acid again, drops into beans or other vegetable-protein in extracting solution, removes the tannic acid of exhausted major part; Remaining extract is removed insolubles in ethanol or methanol solution, obtain the cianidanol crude product, with the impurity in the plumbic acetate precipitation crude product, or makes the cianidanol elaboration with the method for macroporous resin adsorption, ethanol or methanol solution wash-out.
Below be example of the present invention:
1. extract: take by weighing 1 kilogram of CATECHU, be ground into Powderedly, add 5 kilograms in water, heated and boiled 1 hour is filtered, and obtains tan filtrate: drop into 6 kilograms of 10% soybean protein solutions in filtrate, separate out throw out after the stirring, be catechutannic acid albumen; Filter, filtrate decompression is condensed into the thick paste shape, adds 5 kilogram of 95% ethanol, places 12 hours after stirring, and filters, and filtrate decompression reclaims ethanol to the greatest extent, gets a thick medicinal extract; Be heated to medicinal extract with 1 kilogram of distilled water and all dissolve, separate out solids after the cooling, the leaching solids washes with water, drying, a khaki color powder, be the cianidanol crude product, yield is 12%.
2. refining:
Method one is dissolved in above-mentioned crude product in 3 times of calorimetric water, drips plumbic acetate solution and does not separate out to there being a large amount of precipitations; Filter, after filtrate is taken off lead with sodium sulfate, filter, filtrate is placed about 0 ℃, separates out white, needle-shaped crystals, is the cianidanol elaboration, and yield is 45%.
Method two is got a chromatography column, and the macroporous resin of packing into is used a spot of hot water dissolving with crude product, add in the post and wash with 30% ethanolic soln, collect washing fluid, reclaim under reduced pressure is to small volume, low temperature is separated out white, needle-shaped crystals, is the cianidanol elaboration, and yield is 40%.
The present invention uses water as the extraction solvent owing to adopting, low-cost vegetable protein is made main precipitating reagent, and technology is simplified greatly, has eliminated three-waste pollution, cost reduces significantly, and the product yield rises to 5% from 2% of existing technology, and product purity reaches 99%, therefore, very big practical value is arranged.
Claims (4)
1, one from plant, especially from the Chinese medicine CATECHU, extract the Technology of cianidanol, it is characterized in that water is as extracting solvent, combine with tannic acid with vegetable-protein and to generate the tannin albuminate precipitation mode and remove tannic acid in the extracting solution, obtain crude product, with the plumbic acetate precipitator method or the refining cianidanol of macroporous resin adsorption elution method.
2,, when it is characterized in that obtaining the cianidanol crude product, obtain catechutannic acid albumen as said technology in the claim 1..
3, as the protein of said vegetable-protein in the claim 1. for from beans or other plant, obtaining.
4, be ethanol or methyl alcohol as the elutriant in the said macroporous resin adsorption elution method in the claim 1..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85102818 CN85102818A (en) | 1985-04-15 | 1985-04-15 | The novel technology for extracting of cianidanol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 85102818 CN85102818A (en) | 1985-04-15 | 1985-04-15 | The novel technology for extracting of cianidanol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN85102818A true CN85102818A (en) | 1986-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 85102818 Pending CN85102818A (en) | 1985-04-15 | 1985-04-15 | The novel technology for extracting of cianidanol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN85102818A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526170A (en) * | 2011-12-31 | 2012-07-04 | 许学志 | Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition |
| CN111569466A (en) * | 2020-05-09 | 2020-08-25 | 浙江工业大学 | Method for removing tannic acid in Chinese herbal medicine extracting solution |
-
1985
- 1985-04-15 CN CN 85102818 patent/CN85102818A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102526170A (en) * | 2011-12-31 | 2012-07-04 | 许学志 | Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition |
| CN102526170B (en) * | 2011-12-31 | 2015-04-29 | 许学志 | Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition |
| CN111569466A (en) * | 2020-05-09 | 2020-08-25 | 浙江工业大学 | Method for removing tannic acid in Chinese herbal medicine extracting solution |
| CN111569466B (en) * | 2020-05-09 | 2022-06-14 | 浙江工业大学 | Method for removing tannic acid in Chinese herbal medicine extracting solution |
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| SE01 | Entry into force of request for substantive examination | ||
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| WD01 | Invention patent application deemed withdrawn after publication |