Calvacin gel is composed Calvatia gigantea ex Pers and Gentiana macrophylla Pall which usually used for burn and aseptic, lining and cutting wound in traditional Mongolian medicine. The objective of the study to determine technological parameter and total biological active substances and analyze the effect of burn healing Calvacin gel in rats with full-thickness second-degree burn model. Methods: Quantitative determination of the organic acid of the gel of Calvacin was performed using titremeter method respectively. Standard method was used to induce burn wound on in rats and the animals were divided into 4 groups: Control group, Calvacin-1, Calvacin-2, Silver sulfadiazine. After 24 hours of burn model being induced, we have put a thin layer of experimental gel and Silver sulfadiazine once a day. Wound area was measured every three day for 28 days under careful observation, and on the day of 7, 14, 21, 28 blood samples were taken from the animals in order to determine level of VEGF, TGF[beta]1 to evaluate wound healing process. Results: For choosing of appropriate gel former, gels were prepared by 1% and 2% carbomer, 3%, 6% sodium carboxymethyl cellulose. The total content of organic acid 1.3 [+ or -] 0.08%, 1.29 [+ or -] 0.1%,1.28 [+ or -] 0.09% 1.29 [+ or -] 0.09%. The rats treated with Calvacin had significantly increased levels of VEGF (at 14, 21 and 28 day), and TGFfi1 (at 28 day). Conclusions: The current study showed that Calvacin has a burn healing activity. Keywords: Burn Wound; Calvatia Gigantea Batch ex Pers; Gentiana Macrophylla Pall; Traditional Mongolian Medicine.