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Development of Oryza sativa L. by Oryza punctata Kotschy ex Steud. monosomic addition lines with high value traits by interspecific hybridization

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This paper describes the development of monosomic alien addition and disomic introgression lines through a cross between autotetraploid indica rice and Oryza punctata toward tapping valuable traits for rice improvement.

Abstract

Oryza punctata is a distantly related wild Oryza species having BB genome with untapped genetic resources for rice improvement. Low crossability between the cultivated O. sativa and O. punctata restricts the success of transferring many desirable traits into cultivated rice. Artificially induced autotetraploids of an elite breeding line, IR31917-45-3-2, were produced and crossed with O. punctata. Allotriploid F1 plants were backcrossed to IR31917-45-3-2 and generated progenies with extra chromosomes from O. punctata. Twenty BC1F1 and 59 BC2F1 plants were produced with chromosome numbers ranging from 24 (2n) to 29 (2n + 5) and 2n (24) to 26 (2n + 2), respectively. Eleven monosomic alien addition lines (MAALs) were characterized morphologically and cytologically and designated as MAAL 1–12. MAALs were genotyped using O. punctata genome-specific molecular markers and detected chromosome segments inherited from O. punctata. O. punctata introgressions across all the chromosomes of O. sativa were identified except for chromosome 8. The most frequent introgressions were observed in chromosomes 4, 6, 10, and 11, which could be the recombination hotspots between A and B genomes. Some of the qualitative traits such as black hull, purple coleoptile base, purple stigma, long awn, and short grain size from O. punctata were inherited in some disomic introgression lines (DILs). Several DILs inherited genes from O. punctata conferring resistance to brown planthopper, green leafhopper, and diseases such as bacterial blight and blast. This is the first report on successful gene transfer from O. punctata into O. sativa.

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Acknowledgments

We would like to thank Mr. Patricio Carandang, Mr. Eleazar Manalaysay, and Ms. Janice Sapin for providing excellent technical assistance during the experiment. We thank Dr. Sung-Ryul Kim for assisting in Indel marker development through comparative sequence analysis. We thank the editorial team of IRRI Communication for an accurate editing of the manuscript. We are grateful to the Global Rice Science Partnership (GRiSP) program (Grant No. DRPC 2011-134) for financial support to this study.

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  1. Novel Gene Resources Laboratory, Plant Breeding Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    Kshirod K. Jena, Ma. LaRue E. Ballesfin & Ricky B. Vinarao

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  1. Kshirod K. Jena
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  2. Ma. LaRue E. Ballesfin
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Correspondence to Kshirod K. Jena.

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Communicated by L. Jiang.

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122_2016_2745_MOESM1_ESM.tif

Fig. S1 Physical map of O. punctata-specific 111 markers designed for molecular detection of introgression (TIFF 552 kb)

122_2016_2745_MOESM2_ESM.pptx

Fig. S2 Detection of O. punctata segment in the resistant DILs using genome-specific markers. Banding pattern are as follows: P1-IR31917-45-3-2 (red arrow), P2- O. punctata (green arrow), Lanes 1-14 are DILs in BC2F4 generation, M - 100 bp DNA Ladder. Note the presence of O. punctata genome-specific allele (304 bp) in lanes 4, 6, 7, 8, 9, 13 and 14; and 285 bp allele of O. sativa (IR31917-45-3-2) (PPTX 713 kb)

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Jena, K.K., Ballesfin, M.L.E. & Vinarao, R.B. Development of Oryza sativa L. by Oryza punctata Kotschy ex Steud. monosomic addition lines with high value traits by interspecific hybridization. Theor Appl Genet 129, 1873–1886 (2016). https://doi.org/10.1007/s00122-016-2745-8

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