Abstract
The general morphology of the body, including the distribution of putative sensory ciliary cells, was studied using scanning electron microscopy (SEM) in the siboglinid Nereilinum murmanicum Ivanov, 1961 collected from the Barents Sea and at a new, deeper locality in the Greenland Sea outside the known range of this species. The fine features of cuticular structures in N. murmanicum, including the bridle and cuticular plaques from different parts of the body, were described for the first time. Since we have previously shown in the closely related siboglinid Oligobrachia haakonmosbiensis Smirnov, 2000 using SEM and confocal laser scanning microscopy (CLSM) that all epidermal cilia except for the ventral ciliary band belong to sensory cells, we consider all ciliary structures detected in N. murmanicum as sensory. The tentacles, clusters of ciliary cells along the dorsal furrow, areas around the openings of the multicellular glands, papillae, and the ciliary patch located on the cephalic lobe at the base of the tentacles can be regarded as specialized sensory areas. Based on our current knowledge of sensory structures in annelids, a number of assumptions were made about possible functional characteristics of putative sensory structures in siboglinids.
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The datasets analyzed during the current study are available from the corresponding author on request. All data needed are included in the paper.
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Acknowledgements
The authors are grateful to Dr. Olga Zimina (Murmansk Marine Biological Institute, Russian Academy of Sciences, Murmansk, Russia), Dr. Vitaly Semin (P.P. Shirshov Institute of Oceanology, Russian Academy of Sciences, Moscow, Russia) and the staff of the Zoological Institute, Russian Academy of Sciences (ZIN RAS, St. Petersburg, Russia) for their assistance in obtaining the material for the research. Our particular thanks are also due to Dr. Anatoly Petrov (ZIN RAS) for constructive suggestions and linguistic comments that improved the manuscript. The study was carried out using equipment of the Core Facilities Centre “Taxon” at the ZIN RAS. Financial support for this work was provided by budget funding (project 122031100281-5 “Morphological and molecular basis of evolution in protists and key invertebrate groups”).
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RVS prepared specimens for SEM. RVS and OVZ performed microscopical studies, acquired the data, and analyzed the results. OVZ and RVS wrote the main manuscript text, RVS and SAP prepared the figures and contributed to the manuscript. All authors have read and approved the manuscript.
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Smirnov, R.V., Zaitseva, O.V. & Petrov, S.A. Scanning electron microscopic investigation of general morphology and ciliary structures in Nereilinum murmanicum Ivanov, 1961 (Annelida, Siboglinidae). Zoomorphology 143, 13–29 (2024). https://doi.org/10.1007/s00435-023-00638-z
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DOI: https://doi.org/10.1007/s00435-023-00638-z