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A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea

  • Fish Parasitology - Original Paper
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Abstract

A new myxosporean parasite, Myxodavisia jejuensis n. sp. (Myxozoa; Bivalvulida) is described from the urinary bladder of olive flounder Paralichthys olivaceus cultured on Jeju Island, Korea. Two long lateral appendages with whip-like extensions were attached to mature spores of triangular to semi-circular shape. The spores were measured at 13.1 ± 1.1 μm in length, 17.2 ± 1.0 μm in thickness, and 13.1 ± 1.0 μm in width. Two spherical polar capsules, with a diameter of 5.0 ± 0.4 μm, were observed on opposite sides in the middle of the spore. The suture line was straight or slightly sinuous on the middle of spores. The 18S rDNA from M. jejuensis n. sp. was used in BLAST and molecular phylogenetic analysis. The results demonstrated that M. jejuensis n. sp. was closest to Sinuolinea capsularis and that the infection site tropism was correlated with the phylogeny of marine myxosporeans. In addition, we designed specific primers to detect the 18S rDNA gene of M. jejuensis n. sp.; the results showed specific amplification in M. jejuensis n. sp. among the myxosporeans isolated from the urinary bladder of the cultured olive flounder.

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References

  • Aseeva NL (2002) New species of myxosporeans (Myxozoa, Myxosporea) from sculpins of the northern Japan Sea. Acta Parasitol 47:179–189

    Google Scholar 

  • Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83:262–271

    Article  CAS  PubMed  Google Scholar 

  • Bartosova P, Freeman MA, Yokoyama H, Caffara M, Fiala I (2011) Phylogenetic position of Sphaerospora testicularis and Latyspora scomberomori n. gen. n. sp. (Myxozoa) within the marine urinary clade. Parasitology 138:381–393

    Article  CAS  PubMed  Google Scholar 

  • Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552

    Article  CAS  PubMed  Google Scholar 

  • Cho JB, Kim KH (2001) Light and electron microscopic observations of Leptotheca koreana n. sp. (Myxosporea) in the kidney of cultured rockfish Sebastes schlegeli. Dis Aquat Org 46:189–195

    Article  CAS  Google Scholar 

  • Cho JB, Kim KH (2003) Light- and electron-microscope description of Kudoa paralichthys n. sp. (Myxozoa, Myxosporea) from the brain of cultured olive flounder Paralichthys olivaceus in Korea. Dis Aquat Org 55:59–63

    Article  Google Scholar 

  • Cho JB, Kim KH (2004) Light and electron microscopical observations of Parvicapsula anisocaudata (Myxosporea: Parvicapsulidae) from urinary system of cultured olive flounder, Paralichtys olivaceus. J Fish Pathol 17:179–189

    Google Scholar 

  • Cho JB, Kim KH (2016) Light and electron microscopic observations of Ceratomyxa sparusaurati (Myxosporea: Bivalvulida) from the gall bladder of rock bream (Oplegnathus fasciatus). J Fish Pathol 29:7–12

    Article  Google Scholar 

  • Cho JB, Kwon SR, Kim SK, Nam YK, Kim KH (2004) Ultrastructure and development of Ceratomyxa protopsettae Fujita, 1923 (Myxosporea) in the gallbladder of cultured olive flounder, Paralichthys olivaceus. Acta Protozool 43:241–250

    Google Scholar 

  • Cho JB, Huh MD, Kim KH, Kwon SR, Lee MK (2006a) Myxobolus episquamalis (Myxosporea: Myxobolidae) on the scales of wild mullet, Mugil cephalus L, in Korea. J Fish Pathol 19:1–6

    Google Scholar 

  • Cho JB, Lee EH, Kwon SR, Kim CS, Kim KH (2006b) Ceratomyxa oplegnathus n. sp. from the gallbladder of cultured rock bream, Oplegnathus fasciatus. J Fish Pathol 19:109–117

    Google Scholar 

  • Choi HJ, Jee BY, Park MA, Lee DC (2010) Myxobolus episquamalis (Myxosporea: Myxobolidae) on the scales of wild mullet, Mugil cephalus L, in Korea. J Fish Pathol 23:113–118

    Google Scholar 

  • Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9:772

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Davis HS (1917) The Myxosporidia of the Beaufort region, a systematic and biological study. Bull US Bur Fish 35:199–252

    Google Scholar 

  • Diamant A, Whipps CM, Kent ML (2004) A new species of Sphaeromyxa (Myxosporea: Sphaeromyxina: Sphaeromyxidae) in devil firefish, Pterois miles (Scorpaenidae), from the northern Red Sea: morphology, ultrastructure, and phylogeny. J Parasitol 90:1434–1442

    Article  CAS  PubMed  Google Scholar 

  • Fiala I (2006) The phylogeny of myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534

    Article  CAS  PubMed  Google Scholar 

  • Fiala I, Hlavnickova M, Kodadkova A, Freeman MA, Bartosova-Sojkova P, Atkinson SD (2015) Evolutionary origin of Ceratonova shasta and phylogeny of the marine myxosporean lineage. Mol Phylogenet Evol 86:75–89

    Article  PubMed  Google Scholar 

  • Fonseca VG, Carvalho GR, Sung W, Johnson HF, Power DM, Neill SP, Packer M, Blaxter ML, Lambshead PJ, Thomas WK, Creer S (2010) Second-generation environmental sequencing unmasks marine metazoan biodiversity. Nat Commun 1:98

    Article  PubMed  CAS  Google Scholar 

  • Gayevskaya AV, Kovaleva AA (1979) Two new species o Myxosporidia Davisia donecae n. sp. and Ceratomyxa australis n. sp. from the horse mackerel in the south-eastern Atlantic. Biol Mor Vladivos 3:80–83 (in Russian)

    Google Scholar 

  • Gayevskaya AV, Kovaleva AA, Umnova BA (1980) Davisia amoena sp. nov. (Myxosporidia, Sinuolineidae), a parasite of fishes of the family Pleuronectidae from the north-western Atlantic. Parazitologiya 3:276–279

    Google Scholar 

  • Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704

    Article  PubMed  Google Scholar 

  • Hallett SL, Diamant A (2001) Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n. sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Dis Aquat Org 46:197–212

    Article  CAS  Google Scholar 

  • Holzer AS, Sommerville C, Wootten R (2004) Molecular relationships and phylogeny in a community of myxosporeans and actinosporeans based on their 18S rDNA sequences. Int J Parasitol 34:1099–1111

    Article  CAS  PubMed  Google Scholar 

  • Jeon CH, Kim JH (2015) Myxobolus aeglefini (Myxozoa: Myxobolidae) infection in muscles of porous-head eelpout (Bothrocara hollandi). J Fish Pathol 28:79–85

    Article  Google Scholar 

  • Jeon CH, Do JW, Nam UH, Kim WS, Kim JH (2017) Development of PCR method for detecting Kudoa iwatai (Myxozoa: Multivalvulida) from rock bream Oplegnathus fasciatus. Parasitol Res 116:789–796

    Article  PubMed  Google Scholar 

  • Jun SK, Park SI, Choi DL (1988) Studies of Thelohanellus infection in carp, Cyprinus carpio I. Experimental induction of Thelohanellosis. J Fish Pathol 1:111–116

    Google Scholar 

  • Kabata Z (1962) Five new species of Myxosporidia from marine fishes. Parasitology 52:177–186

    Article  Google Scholar 

  • Kim YG, Kim DY (2005) Studies on myxosporidian parasites from Korean freshwater fishes 1.Myxosporidian parasites from Dongjin river, Jeonbuk province of Korea. J Fish Pathol 18:1–18

    Google Scholar 

  • Kim YG, Park SU, Choi MC (2002) Studies on myxosporidian parasites from Korean fishes. J Fish Pathol 15:105–110

    Google Scholar 

  • Kim WS, Kim JH, Jang MS, Jung SJ, Oh MJ (2013a) Infection of wild mullet (Mugil cephalus) with Myxobolus episquamalis in Korea. Parasitol Res 112:447–451

    Article  PubMed  Google Scholar 

  • Kim WS, Kim JH, Oh MJ (2013b) Morphologic and genetic evidence for mixed infection with two Myxobolus species (Myxozoa: Myxobolidae) in gray mullets, Mugil cephalus, from Korean waters. Korean J Parasitol 51:369–373

    Article  PubMed  PubMed Central  Google Scholar 

  • Kim JJ, Ryu S, Lee H (2018) Foodborne illness outbreaks in Gyeonggi province, Korea, following seafood consumption potentially caused by Kudoa septempunctata between 2015 and 2016. Osong Public Health Res Perspect 9:66–72

    Article  PubMed  PubMed Central  Google Scholar 

  • Kovaleva AA, Rodjuk GN (1991) New members of myxosporidia (Cnidospora, Myxosporea) from fishes in the Falkland-Patagonian region. Parazitologiya 25:549–551

    Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kwon SR, Kim HJ (2011) Thelohanellus misgurni (Kudo, 1919) infection on the fins of Chinese muddy loach Misgurnus mizolepis. J Fish Pathol 24:167–171

    Article  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    CAS  PubMed  Google Scholar 

  • Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156

    Article  Google Scholar 

  • Lom J, Dykova I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36

    Article  Google Scholar 

  • Love MS, Moser M (1976) Davisia reginae sp. nov. (Protozoa: Myxosporidia) from four Californian marine fishes. J Parasitol 62:982–983

    Article  CAS  PubMed  Google Scholar 

  • Padma Dorothy K, Kalavati C, Vaidchi J (1998) Three new species of myxozoans from teleosts of bay of Bengal. Riv Parassitol 15:67–72

    Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  PubMed  PubMed Central  Google Scholar 

  • Sarkar NK (2010) Myxodavisia haldarae sp. n. and Ceratomyxa sardinellae sp. n. (Myxosporea, Bivalvulida) from the gall bladder of Indian sardine Sardinella longiceps collected in the Bay of Bengal. Protistology 6:290–296

    Google Scholar 

  • Seo JS, Jeon EJ, Kim MS, Woo SH, Kim JD, Jung SH, Park MA, Jee BY, Kim JW, Kim YC, Lee EH (2012) Molecular identification and real-time quantitative PCR (qPCR) for rapid detection of Thelohanellus kitauei, a myxozoan parasite causing intestinal giant cystic disease in the Israel carp. Korean J Parasitol 50:103–111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shin SP, Jee H, Han JE, Kim JH, Choresca CH, Jun JW, Kim DY, Park SC (2011) Surgical removal of an anal cyst caused by a protozoan parasite (Thelohanellus kitauei) from a koi (Cyprinus carpio). J Am Vet Med Assoc 238:784–786

    Article  PubMed  Google Scholar 

  • Shin SP, Nguyen VG, Jeong JM, Jun JW, Kim JH, Han JE, Baeck GW, Park SC (2014) The phylogenetic study on Thelohanellus species (Myxosporea) in relation to host specificity and infection site tropism. Mol Phylogenet Evol 72:31–34

    Article  PubMed  Google Scholar 

  • Shin SP, Shirakashi S, Hamano S, Kato K, Lasso LT, Yokoyama H (2016) Phylogenetic study of the genus Kudoa (Myxozoa: Multivalvulida) with a description of Kudoa rayformis sp. nov. from the trunk muscle of Pacific sierra Scomberomorus sierra. Mol Phylogenet Evol 98:337–345

    Article  PubMed  Google Scholar 

  • Shin SP, Jin CN, Sohn HC, Lee J (2018a) Parvicapsula curvatura n. sp. in cultured olive flounder Paralichthys olivaceus and phylogenetic characteristics of the genus Parvicapsula. Dis Aquat Org 130:199–207

    Article  CAS  Google Scholar 

  • Shin SP, Sohn HC, Jin CN, Kang BJ, Lee J (2018b) Molecular diagnostics for verifying an etiological agent of emaciation disease in cultured olive flounder Paralichthys olivaceus in Korea. Aquaculture 493:18–25

    Article  Google Scholar 

  • Shin SP, Jin CN, Sohn HC, Lee J (2019) Sinuolinea capsularis (Myxosporea: Sinuolineidae) isolated from urinary bladder of cultured olive flounder Paralichthys olivaceus. Korean J Parasitol 57:127–134

    Article  PubMed  PubMed Central  Google Scholar 

  • Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Söding J, Thompson JD, Higgins DG (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal omega. Mol Syst Biol 7:539

    Article  PubMed  PubMed Central  Google Scholar 

  • Song JY, Choi JH, Choi HS, Jung SH, Park MA (2013) Monitoring of Kudoa septempunctata in cultured olive flounder and wild fish in Jeju island during 2012. J Fish Pathol 26:129–137

    Article  Google Scholar 

  • Yang Y, Xiong J, Zhou Z, Huo F, Miao W, Ran C, Liu Y, Zhang J, Feng J, Wang M, Wang M, Wang L, Yao B (2014) The genome of the myxosporean Thelohanellus kitauei shows adaptations to nutrient acquisition within its fish host. Genome Biol Evol 6:3182–3198

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao Y, Ma C, Song W (2002) Davisia hexagrammi n. sp. (Myxosporea: Sinuolineidae) parasitic in the urinary bladder of marine fishes from the coast of the Yellow sea and Bohai bay, China. Syst Parasitol 52:153–158

    Article  PubMed  Google Scholar 

  • Zhao Y, Zhou Y, Kent ML, Whipps CM (2008) Replacement of the preoccupied name Davisia Laird 1953 and description of a new myxozoan species (Myxosporea: Sinuolineidae) from Sebastiscus marmoratus (Cuvier, 1829) in the east China Sea. J Parasitol 94:269–279

    Article  PubMed  Google Scholar 

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Funding

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03932598) and is part of the project titled ‘Fish Vaccine Research Center,’ funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Department of Marine Life Science & Fish Vaccine Research Center, Jeju National University, Jeju, Jeju Self-Governing Province, 63243, Republic of Korea

    Sang Phil Shin, Chang Nam Jin, Han Chang Sohn & Jehee Lee

  2. Faculty of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, 794-8555, Japan

    Hiroshi Yokoyama

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  1. Sang Phil Shin
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  2. Chang Nam Jin
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  3. Han Chang Sohn
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  4. Hiroshi Yokoyama
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Correspondence to Jehee Lee.

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Shin, S.P., Jin, C.N., Sohn, H.C. et al. A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea. Parasitol Res 118, 3105–3112 (2019). https://doi.org/10.1007/s00436-019-06454-z

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