Abstract
The genus Gyrodactylus von Nordmann, 1832 represents one of the most diverse and widespread taxa within Monogenea, with approximately 500 species described worldwide. Thirty-three species of Gyrodactylus have been recorded in Mexico, and in the last two decades, at least 26 new species have been described mainly from freshwater fish families such as poeciliids, goodeids, profundulids, characids, and cichlids. In this study, we describe two new species of Gyrodactylus infecting freshwater cyprinids based on morphological and molecular characteristics. Gyrodactylus ticuchi n. sp. and Gyrodactylus tobala n. sp. were recovered from Notropis moralesi de Buen and N. imeldae Cortés, respectively, captured in five localities from the State of Oaxaca, Mexico. The new species differ slightly from their congeners in the morphology of the haptoral hard parts and the male copulatory organ. Sequences of the Internal Transcribed Spacers rDNA (ITS1-5.8S-ITS2), cytochrome oxidase subunit I (cox1), and the D2 + D3 domains of the large subunit (28S rDNA) were obtained from multiple specimens and analyzed using Maximum Likelihood (ML) and Bayesian Inference (BI). Phylogenetic hypotheses using ITS rDNA, cox1, and 28S rDNA genes recovered two new species of Gyrodactylus from N. moralesi and N. imeldae; we briefly discuss their phylogenetic relationship with other congeners. These gyrodactylids represent the first species described in species of Notropis from southern Mexico, the cyprinids exhibiting the southernmost distribution in the New World.
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Acknowledgments
We are grateful to Florencio Martínez, Rubén Martínez, and Nina Martínez for their help during field work. We thank Cristina Bárcenas for the help to obtain DNA sequences in INECOL and the institute’s General Direction for covering the sequencing costs. MCR acknowledges the support of the Programa de Doctorado en Ciencias, INECOL and CONACyT for granting a scholarship to complete his PhD program. To conduct this work, permits were obtained from the Mexican Ministry of the Environment and Natural Resources (SEMARNAT) under scientific collection permits SGPA/DGVS/02967/14.
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Pinacho-Pinacho, C.D., Calixto-Rojas, M., García-Vásquez, A. et al. Species delimitation of Gyrodactylus (Monogenea: Gyrodactylidae) infecting the southernmost cyprinids (Actinopterygii: Cyprinidae) in the New World. Parasitol Res 120, 831–848 (2021). https://doi.org/10.1007/s00436-020-06987-8
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DOI: https://doi.org/10.1007/s00436-020-06987-8