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Specific arbuscular mycorrhizal fungi associated with non-photosynthetic Petrosavia sakuraii (Petrosaviaceae)

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Abstract

Mycorrhizal fungi in roots of the achlorophyllous Petrosavia sakuraii (Petrosaviaceae) were identified by molecular methods. Habitats examined were plantations of the Japanese cypress Chamaecyparis obtusa in Honshu, an evergreen broad-leaved forest in Amami Island in Japan and a mixed deciduous and evergreen forest in China. Aseptate hyphal coils were observed in root cortical cells of P. sakuraii, suggesting Paris-type arbuscular mycorrhiza (AM). Furthermore, hyphal coils that had degenerated to amorphous clumps were found in various layers of the root cortex. Despite extensive sampling of P. sakuraii from various sites in Japan and China, most of the obtained AM fungal sequences of the nuclear small subunit ribosomal RNA gene were nearly identical and phylogenetic analysis revealed that they formed a single clade in the Glomus group A lineage. This suggests that the symbiotic relationship is highly specific. AM fungi of P. sakuraii were phylogenetically different from those previously detected in the roots of some mycoheterotrophic plants. In a habitat in C. obtusa plantation, approximately half of the AM fungi detected in roots of C. obtusa surrounding P. sakuraii belonged to the same clade as that of P. sakuraii. This indicates that particular AM fungi are selected by P. sakuraii from diverse indigenous AM fungi. The same AM fungi can colonize both plant species, and photosynthates of C. obtusa may be supplied to P. sakuraii through a shared AM fungal mycelial network. Although C. obtusa plantations are widely distributed throughout Japan, P. petrosavia is a rare plant species, probably because of its high specificity towards particular AM fungi.

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Acknowledgements

We thank Ryozo Hayashi, Tatsuya Iwai, Masayuki Matsui, Teruo Nakajima and Hiroshi Yamashita for their kind help during the field work and Hiroko Abe and Shohei Fujimomri for technical assistance. This study was supported by the Global COE Program ‘Advanced utilization of fungus/mushroom resources for sustainable society in harmony with nature’ from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by Grants-in-Aid to Scientific Research from the Japan Society for Promotion of Science (Nos. 21370038 and 21510240).

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Authors and Affiliations

  1. Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8553, Japan

    Masahide Yamato, Takahiro Yagame, Norihiro Shimomura & Koji Iwase

  2. Department of Biology, Faculty of Education, Gifu University, Gifu, Japan

    Hiroshi Takahashi

  3. Tsukuba Botanical Garden, National Museum of Nature and Science, Tsukuba, Japan

    Yuki Ogura-Tsujita & Tomohisa Yukawa

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  1. Masahide Yamato
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Correspondence to Masahide Yamato.

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Yamato, M., Yagame, T., Shimomura, N. et al. Specific arbuscular mycorrhizal fungi associated with non-photosynthetic Petrosavia sakuraii (Petrosaviaceae). Mycorrhiza 21, 631–639 (2011). https://doi.org/10.1007/s00572-011-0373-3

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  • DOI: https://doi.org/10.1007/s00572-011-0373-3

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