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Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice

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Abstract

Using ethno-medicinal plants is the oldest way of mankind to treat the several diseases. Due to the rapid growth of global interest in use of medicinal plants, their effects and safety evaluation have become substantial. In this study, Alyssum meniocoides (AM) Boiss aqueous extract was extracted to investigate its nephroprotective activity on renal structural and biochemical changes in streptozotocin-induced diabetic nephrotoxicity in male mice. In this study, 80 mice were used. Diabetes was experimentally induced by intraperitoneal injection of streptozotocin (STZ) in 70 mice. Fasting blood glucose (FBG) levels were assessed everyday by glucometer strips. Mice with plasma glucose level > 250 mg/dL were considered diabetic. After 3 days, they were divided randomly into 8 groups. Groups 1 and 2 served as non-diabetic and untreated diabetic controls, respectively. Group 3 received 40 mg/kg glibenclamide orally. Groups 4, 5, 6, 7, and 8 were given 10, 20, 40, 80, and 160 mg/kg, respectively of AM for 20 days orally. At the 20th day, the mice were dissected, blood and kidney samples collected for biochemical and histological analysis. Histologically, several doses of AM could significantly (p ≤ 0.05) decrease the volume and length of the renal structures as compared to the untreated group. Biochemically, AM at all doses could significantly (p ≤ 0.05) reduce the raised levels of urea and creatinine and increased SOD and catalase (CAT) levels as compared to the untreated group. In conclusion, AM has nephroprotective property, thereby reducing the causation of diabetes in experimental mice.

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References

  • Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684

    Chapter  Google Scholar 

  • Braendgaard H, Gundersen HJ (1986) The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods 18(1–2):39–78

    Article  PubMed  CAS  Google Scholar 

  • Breyer MD, Bottinger E, Brosius FC, Coffman TM, Harris RC, Heilig CW, Sharma K, AMDCC (2005) Mouse models of diabetic nephropathy. J Am Soc Nephrol 16(1):27–45

    Article  PubMed  Google Scholar 

  • Brosius FC, Alpers CE, Bottinger EP, Breyer MD, Coffman TM, Gurley SB, Harris RC, Kakoki M, Kretzler M, Leiter EH, Levi M, McIndoe RA, Sharma K, Smithies O, Susztak K, Takahashi N, Takahashi T (2009) Mouse models of diabetic nephropathy. J Am Soc Nephrol 20(12):2503–2512

    Article  PubMed  PubMed Central  Google Scholar 

  • Dhodi DK, Bhagat SB, Pathak D, Patel SB (2014) Drug-induced nephrotoxicity. Int J Basic Clin Pharmacol 3(4):591–597

    Article  Google Scholar 

  • Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2016) Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Current Med Pharm Res 7(6):367–371

    Google Scholar 

  • Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R (2017) Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rew Res 8(1):13–17

    Google Scholar 

  • Ghaderiana SM, Mohtadia A, Rahiminejada MR, Baker AJM (2007) Nickel and other metal uptake and accumulation by species of Alyssum (Brassicaceae) from the ultramafics of Iran. Environ Pollut 145(1):293–298

    Article  CAS  Google Scholar 

  • Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263

    Article  Google Scholar 

  • Goodarzi N, Zangeneh MM, Zangeneh A, Najafi F, Tahvilian R (2017) Protective effects of ethanolic extract of Allium Saralicum R.M. Fritsch on CCl4-induced hepatotoxicity in mice. J Rafsanjan Univ Med Sci 16(3):227–238

    Google Scholar 

  • Goodarzi N, Zangeneh MM, Zangeneh A (2018) The effect of ethanolic extract of Allium Saralicum R.M. Fritsch on diabetic hepatopathy in male mice. Sci Res J Shahed Univ 25(132):21–30

    Google Scholar 

  • Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A (1992) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Acta pathologica microbiologica immunologica. Scandinavica 96(5):379–394

    Google Scholar 

  • Hachi M, Ouafae B, Hachi T, Mohamed EB, Imane B, Atmane R, Zidane L (2016) Contribution to the ethnobotanical study of antidiabetic medicinal plants of the Central Middle Atlas region (Morocco). Lazaroa 37:135–144

    Article  Google Scholar 

  • Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463

    Article  Google Scholar 

  • Kitabchi AE, Umpierrez GE, Miles JM, Fisher JN (2009) Hyperglycemic crises in adult patients with diabetes. Diabetes Care 32(7):1335–1343

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kraynak AR, Storer RD, Jensen RD, Kloss MW, Soper KA, Clair JH, DeLuca JG, Nichols WW, Eydelloth RS (1995) Extent and persistence of streptozotocin-induced DNAdamage and cell proliferation in rat kidney as determined by in vivo alkaline elution and BrdUrd labeling assays. Toxicol Appl Pharmacol 135(2):279–286

    Article  PubMed  CAS  Google Scholar 

  • Le-Devehat C, Khodabandehlou T, Vimeux M (2001) Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc 25(2):43–48

    PubMed  CAS  Google Scholar 

  • Lenzen S (2008) The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 51(2):216–226

    Article  PubMed  CAS  Google Scholar 

  • Mandarim-de-Lacerda CA (2003) Stereological tools in biomedical research. An Acad Bras Cienc 75(4):469–486

    Article  PubMed  Google Scholar 

  • Martin JP Jr, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336

    Article  PubMed  CAS  Google Scholar 

  • Mishra S, Ranjan-Pani S, Sahoo S (2014) Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha. Pharm Res 6(3):210–217

    Google Scholar 

  • Mohana-Lakshmi S, Usha-Kiran-Reddy T, Sandhya-Rani KS (2012) A review on medicinal plants for nephroprotective activity. Asian J Pharm Clin Res 5(4):8–14

    Google Scholar 

  • Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A (2017) Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res 3(2):1360–1362

  • Najafi F, Tahvilian R, Zangeneh MM, Zangeneh A, Moradi R (2016) Screening of essential oil of Allium sativum for antibacterial effects against Bacillus subtilis. Int J Rec Sci Res 7(11):14172–14176

    Google Scholar 

  • Najafi F, Goodarzi N, Zangeneh MM, Zangeneh A, Hagh-Nazari L (2017) Antidiabtic and hepatoprotective effects of bitter fraction of Stevia rebaudiana alcoholic extract on streptozotocin-induced diabetic male mice. J Rafsanjan Univ Med Sci 16(6):493–504

    Google Scholar 

  • Palm F, Ortster H, Hansell P, Liss P, Carlsson PO (2004) Differentiating between effects of streptozotocin per se and subsequent hyperglycemia on renal function and metabolism in the streptozotocin diabetic rat model. Diabetes Metab Res Rev 20(6):452–459

    Article  PubMed  Google Scholar 

  • Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A (2017) Cucurbita moschata: a plant with antibacterial properties. Int J Current Med Pharm Res 3(2):1356–1359

    Google Scholar 

  • Pooyanmehr M, Zangeneh MM, Zangeneh A, Almasi M (2017) Effect of Verbascum thapsus aqueous extract on Escherichia coli O157:H7. Online J Vet Res 21(9):580–583

    Google Scholar 

  • Rerup CC (1970) Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev 22(4):485–518

    PubMed  CAS  Google Scholar 

  • Schetz M, Dasta J, Goldstein S, Golper T (2005) Drug-induced acute kidney injury. Curr Opin Crit Care 11(6):555–565

    Article  PubMed  Google Scholar 

  • Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543

    Article  Google Scholar 

  • Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M (2017) Chemical composition and screening of antibacterial activity of essential oil of Pistacia khinjuk against two selected pathogenic bacteria. Ann Trop Med Public Health 10(5):1159–1164

    Article  Google Scholar 

  • Tay YC, Wang Y, Kairaitis L, Rangan GK, Zhang C, Harris DCH (2005) Can murine diabetic nephropathy be separated from superimposed acute renal failure? Kidney Int 68(1):391–398

    Article  PubMed  Google Scholar 

  • Tesch GH, Allen TJ (2007) Rodent models of streptozotocin induced diabetic nephropathy. Nephrology (Carlton) 12(3):261–266

    Article  Google Scholar 

  • Tiong HY, Huang P, Xiong S, Li Y, Vathsala A, Zink D (2014) Drug-induced nephrotoxicity: clinical impact and preclinical in vitro models. Mol Pharm 11(7):1933–1948

    Article  PubMed  CAS  Google Scholar 

  • Weiss RB (1982) Streptozocin: a review of its pharmacology, efficacy, and toxicity. Cancer Treat Rep 66(3):427–438

    PubMed  CAS  Google Scholar 

  • Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R (2017) Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem 6(1):251–259

    Google Scholar 

  • Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol. https://doi.org/10.1007/s00580-018-2674-9

  • Zangeneh MM, Zangeneh AA, Tahvilian R, Moradi R (2018b) Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice. https://doi.org/10.1007/s00580-018-2707-4

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Authors and Affiliations

  1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Mohammad Mahdi Zangeneh

  2. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Mahdi Zangeneh, Akram Zangeneh, Reza Tahvilian & Rohallah Moradi

  3. Department of Basic Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

    Akram Zangeneh

  4. Department of Clinical sciences, Faculty of Veterinary Medicine, Kurdistan Islamic Azad University, Sanandaj, Kurdistan, Iran

    Hossein Amiri

  5. Department of Clinical sciences, Faculty of Veterinary Medicine, Tehran Islamic Azad University, Tehran, Iran

    Nassim Amiri

  6. Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Hossein Zhaleh

  7. Department of Basic Sciences, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran

    Payam Razeghi Tehrani

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  1. Mohammad Mahdi Zangeneh
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Correspondence to Mohammad Mahdi Zangeneh.

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Zangeneh, M.M., Zangeneh, A., Amiri, H. et al. Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice. Comp Clin Pathol 27, 1147–1154 (2018). https://doi.org/10.1007/s00580-018-2712-7

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