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Pollen morphology in delimiting subgenera and species of the genus Cybianthus s.l. (Myrsinoideae–Primulaceae)

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Abstract

Pollen grains of species of the subgenera of Cybianthus s.l. were analyzed in order to determine if pollen morphology is consistent with the currently accepted infrageneric classification, and if it can provide characters useful for distinguishing species or groups of species. Acetolyzed pollen grains were analyzed, measured, described and photomicrographed, while non-acetolyzed pollen grains were analyzed using scanning electron microscopy. The pollen grains of all species investigated were isopolar monads, small- to medium-sized, subprolate or prolate, 3-colporate, and rugulate or rugulate-perforate. The different analytical methods (statistical analyses, exploratory analysis of the data and cluster analysis) used demonstrated that pollen morphology is informative in delimiting this genus. However, the pollen characters are generally quite uniform among the subgenres of Cybianthus s.l. Thus, the use of the pollen attributes in the separation of the subordinate species to different subgenera is not effective. Pollen morphology did not give support to current subgeneric systematics, but it did support the genus Cybianthus s.l.

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Acknowledgements

We are grateful to technic of the Department of Invertebrates of the National Museum of the Federal University of Rio de Janeiro, for providing technical assistance with the scanning electron microscopy. This study received financial support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, National Council for Scientific and Technological Development) and from the Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ, Foundation for the Support of Research in the State of Rio de Janeiro).

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Authors and Affiliations

  1. Museu Nacional, Laboratório de Palinologia, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, Rio de Janeiro, RJ, 20940-040, Brazil

    Ana C. da Silva Fourny, Cláudia B. F. Mendonça & Vania Gonçalves-Esteves

  2. Laboratório de Botânica, Departamento de Biologia, Centro de Ciências Exatas, Naturais e da Saúde, Universidade Federal do Espírito Santo, Alto Universitário, s/nº, Alegre, ES, 29500 000, Brazil

    Tatiana T. Carrijo

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  1. Ana C. da Silva Fourny
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  2. Tatiana T. Carrijo
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  3. Cláudia B. F. Mendonça
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  4. Vania Gonçalves-Esteves
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Correspondence to Vania Gonçalves-Esteves.

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Appendix

Appendix

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da Silva Fourny, A.C., Carrijo, T.T., Mendonça, C.B.F. et al. Pollen morphology in delimiting subgenera and species of the genus Cybianthus s.l. (Myrsinoideae–Primulaceae). Plant Syst Evol 304, 535–548 (2018). https://doi.org/10.1007/s00606-018-1498-z

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