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Loktanella soesokkakensis sp. nov., isolated from the junction between the North Pacific Ocean and a freshwater spring

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Abstract

A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK1-5T, was isolated from the junction between the North Pacific Ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK1-5T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DSSK1-5T fell within the clade comprising Loktanella species, clustering consistently with the type strains of Loktanella hongkongensis and Loktanella cinnabarina, with which it exhibited 98.9 and 98.4 % sequence similarity values, respectively. Sequence similarities to the type strains of the other recognized Loktanella species were 94.0–96.2 %. Strain DSSK1-5T was found to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids of strain DSSK1-5T were identified as phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified aminolipid. The DNA G+C content of strain DSSK1-5T was determined to be 67.6 mol% and its mean DNA–DNA relatedness values with L. hongkongensis JCM 12479T and L. cinnabarina JCM 18161T were 19 and 23 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain DSSK1-5T is separated from other Loktanella species. On the basis of the data presented, strain DSSK1-5T is proposed to represent a novel species of the genus Loktanella, for which the name Loktanella soesokkakensis sp. nov. is proposed. The type strain is DSSK1-5T (= KCTC 32425T = CECT 8367T).

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Acknowledgments

This work was supported by a grant from the National Institute of Biological Resources (NIBR) funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK 21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park & Jung-Hoon Yoon

  2. Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, South Korea

    Jung-Sook Lee & Keun-Chul Lee

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  1. Sooyeon Park
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  2. Jung-Sook Lee
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  3. Keun-Chul Lee
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  4. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

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Park, S., Lee, JS., Lee, KC. et al. Loktanella soesokkakensis sp. nov., isolated from the junction between the North Pacific Ocean and a freshwater spring. Antonie van Leeuwenhoek 104, 397–404 (2013). https://doi.org/10.1007/s10482-013-9962-9

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