Abstract
As a significant constituent in biosphere, bacteria have a great influence on human activity. The detection of pathogen bacteria is closely related to the human health. However, the traditional methods for detection of pathogenic bacteria are time-consuming and difficult for quantification, although they are practical and reliable. Therefore, novel strategies for rapid, sensitive, and cost-effective detection are in great demand. Aptamer is a kind of oligonucleotide that selected by repeated screening in vitro or systematic evolution of ligands by exponential enrichment (SELEX) technology. Over the past years, owing to high affinity and specificity of aptamers, a variety of aptamer-based biosensors have been designed and applied for pathogen detection. In this review, we have discussed the recent advances on the applications of aptamer-based biosensors in detection of pathogenic bacteria. In addition, we also point out some problems in current methods and look forward to the further development of aptamer-based biosensors for pathogen detection.
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Acknowledgements
This work was supported by the National Natural Science Fund of China (81903369), Natural Science Foundation of Hunan Province (2019JJ50491, 2020JJ4527), and the Fund of Hengyang Key Laboratory (2018KJ110).
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Dr. Hao Liang was funded by Natural Science Foundation of Hunan Province (2019JJ50491) and National Natural Science Foundation of China (81903369). Dr. Lili Chen was funded by Natural Science Foundation of Hunan Province (2020JJ4527) and Fund of Hengyang Key Laboratory (2018KJ110).
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Li, D., Liu, L., Huang, Q. et al. Recent advances on aptamer-based biosensors for detection of pathogenic bacteria. World J Microbiol Biotechnol 37, 45 (2021). https://doi.org/10.1007/s11274-021-03002-9
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DOI: https://doi.org/10.1007/s11274-021-03002-9