Abstract
We describe Allocanariomyces tritici gen et sp. nov. and Achaetomium aegilopis sp. nov. as seed endophytes of Triticum boeoticum and Aegilops triuncialis, respectively, in the west and northwestern Iran using morphological traits and sequences of the internal transcribed spacer regions 1 and 2 including the intervening 5.8S nuclear ribosomal DNA (ITS), partial nuclear 28S ribosomal DNA (LSU), β-tubulin (TUB2), and the second-largest subunit of DNA-directed RNA polymerase II (RPB2) gene. Chaetomium carinthiacum, C. iranianum, and C. truncatulum are also combined here under the new genus, Parachaetomium. Allocanariomyces is differentiated from Canariomyces, its closest relative, by having solitary and glabrous ascomata, cells of the perithecial wall forming a textura epidermoidea, stalked asci, densely granular ascospores with a distinct subapical germ pore, and producing only solitary conidia. Parachaetomium is characterized by distinctly ostiolate ascomata and equi- or inequilaterally fusiform, typically less than 13-μm-long ascospores with an oblique or subapical germ pore. Achaetomium aegilopis is mainly distinguished from A. strumarium, its closest relative, by possessing brown, ascomata, hyaline ascomatal hairs covered with hyaline crystals, hyaline chlamydospores, and lacking an anamorph.
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Data availability
All sequence data generated in this study (Table 1) are available at GenBank (https://www.ncbi.nlm.nih.gov/genbank/). Alignments can be accessed via electronic supplementary material and TreeBASE (http://www.treebase.org).
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The authors are grateful to Ms. Z. Ghanbari for technical assistance.
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This work was supported by a research grant from the Iranian National Science Foundation (96010651).
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Samples were collected by B. Asgari and M. Mehrabi. Experiments, data analysis, and original draft preparation were conducted by M. Mehrabi. B. Asgari carried out data analysis and review and editing of the manuscript. R. Zare designed the research and revised the manuscript. All authors read and approved the final manuscript.
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Mehrabi, M., Asgari, B. & Zare, R. Description of Allocanariomyces and Parachaetomium, two new genera, and Achaetomium aegilopis sp. nov. in the Chaetomiaceae. Mycol Progress 19, 1415–1427 (2020). https://doi.org/10.1007/s11557-020-01636-x
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DOI: https://doi.org/10.1007/s11557-020-01636-x