Abstract
As a result of molecular phylogenetic analyses and morphological studies, two new species of the genus Pseudosperma, namely P. fulvidiscum and P. singulare, were discovered in Wuzhishan Nature Reserve of Hainan Province of China. The two new species are distinct from other known Pseudosperma species in the phylogram inferred from a combined nuclear ribosomal internal transcribed spacer region (ITS), the nuclear ribosomal large subunit (nrLSU), and the nuclear second-largest subunit of RNA polymerase II (RPB2) sequence data. Muscarine contents of the two new species were detected using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach. In addition, a new combination, P. rubrobrunneum, was proposed based on phylogenetic evidence.
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Data availability
The sequence data generated in this study are deposited in NCBI GenBank.
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Acknowledgements
The authors thank Dr. Shuai Jiang and Mr. Yong-Qing Fu (Yinggeling Substation, Hainan Tropical Rainforest National Park) for their kind help in field works, to Dr. Fei Xu (Ningxia Hui Autonomous Region Center for Disease Control and Prevention) for his kind guidance in toxin detection, and Mr. Chuang-Teng Huang (Hainan Academy of Forestry and Hainan Academy of Mangrove) for his kind help in the identification of host plant.
Funding
This study was supported by the Hainan Basic and applied research project for cultivating high-level talents (No. 2019RC230), the National Natural Science Foundation of China (Nos. 31860009, 31400024, and 3190012), the Natural Science Foundation of Shandong Province (ZR2019PC028), and the Innovative Research Projects for Graduate Students in Hainan Province, China (No. HYYS2020-42).
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Conceptualization: Y-G.F. and W-J.Y. Methodology: L-N.Z. Performing the experiment: L-N.Z. Formal analysis: L-N.Z., W-J.Y., and L-S.D. Resources: Y-G.F., L-S.D., J-H.H., N-K.Z., and Y-P.G. Writing—original draft preparation: L-N.Z. Writing—review and editing: Y-G.F. and W-J.Y. Supervision: Y-G.F. Project administration: Y-G.F. Funding acquisition: Y-G.F. and L-S.D. All authors have read and agreed to the published version of the manuscript.
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Zhao, LN., Yu, WJ., Deng, LS. et al. Phylogenetic analyses, morphological studies, and muscarine detection reveal two new toxic Pseudosperma (Inocybaceae, Agaricales) species from tropical China. Mycol Progress 21, 75 (2022). https://doi.org/10.1007/s11557-022-01822-z
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DOI: https://doi.org/10.1007/s11557-022-01822-z