Abstract
A colourless hyphomycetous fungus was found on living leaves of Peristrophe bicalyculata in India. Molecular phylogenetic analyses of several loci (LSU-RPB2-ITS) indicated a relationship with cercosporoid fungi (Mycosphaerellaceae). Since there is no other known lineage with similar morphology or DNA sequences, the new genus Neocercosporella is proposed with N. peristrophes comb. nov., based on Cercosporella peristrophes as type species. Pseudocercosporella andrographidis is recognized as new synonym. Similar ultrastructure of conidiogenous loci and hila confirm that N. peristrophes and P. andrographidis are conspecific. The conidiogenous loci are conical and have a small rim-like depression on the top encircling a small flat protuberant structure, which differs from other closely related members of the ramularioid complex. Neocercosporella and Cercosporella s. str. are paraphyletic as are several other genera in the Mycosphaerellaceae. Accordingly, we propose three new genera and four new combinations. The new genera are Neoacervuloseptoria gen. nov., Neocercosporella gen. nov. and Neoramulariopsis gen. nov. The new combinations are Neoacervuloseptoria fraxini comb. nov., Neocercosporella peristrophes comb. nov., Neoramulariopsis catenulata comb. nov. and Neoramulariopsis unguis-cati comb. nov.
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Data availability
Sequences generated in this study have been deposited in GenBank with the accession numbers listed in Table 1. The specimen studied in this work was deposited in the Ajrekar Mycological Herbarium (AMH), Agharkar Research Institute (ARI), Pune, Maharashtra, India.
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Acknowledgements
The authors are indebted to anonymous reviewers for helpful comments and the curator of AMH for accepting material and providing an accession number. We are also thankful to the Head, CAS in Botany, Banaras Hindu University, Varanasi, for instrumental facilities.
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RS thanks Science & Engineering Research Board (SERB), Department of Science & Technology (DST), Govt. of India (Scheme No. CRG/2020/006053) and Institution of Eminence (IoE) Scheme, Ministry of Human Resource and Development (MHRD), Govt. of India (Scheme No.6031) for providing financial support.
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All authors contributed to the conception and design of the study. Sanjay Yadav collected samples, tried to cultivate strains, isolated DNA and prepared samples for sequencing. Sanjeet Kumar Verma and Gargee Singh developed morphological features and surveyed literature. Raghvendra Singh developed photo plates, performed phylogenetic analyses and developed the discussion part of the manuscript. Prakash Kushwaha wrote the first draft of the manuscript. All the authors contributed to previous drafts of the manuscript and read and approved the final draft of the manuscript.
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Yadav, S., Singh, R., Verma, S.K. et al. Addition of three new lineages in Mycosphaerellaceae: Neoacervuloseptoria gen. nov., Neocercosporella gen. nov. and Neoramulariopsis gen. nov.. Mycol Progress 22, 26 (2023). https://doi.org/10.1007/s11557-023-01871-y
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DOI: https://doi.org/10.1007/s11557-023-01871-y