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Determination of chemical composition and antimicrobial, antioxidant and cytotoxic activities of lichens Parmelia conspersa and Parmelia perlata

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Abstract

The aim of this study is to investigate the chemical composition of extracts of the lichens Parmelia conspersa and Parmelia perlata and their antimicrobial, antioxidant, and anticancer activities. The phytochemical analysis of the acetone extracts of two Parmelia lichens was determined by (HPLC-UV) method. The predominant phenolic compounds in these extracts were norstictic acid and usnic acids in P. conspersa, while salazinic acid and stictic acid were the major metabolites detected in P. perlata. Besides these compounds, the tested extracts of these lichens contain atranorin and chloroatranorin. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, chelating activity, and inhibitory activity towards lipid peroxidation. The lichen extracts demonstrated important antimicrobial activity against eight strains with MIC values from 19.53 to 312.5 µg/mL. Cytotoxic effects of lichens were tested against Hep2c, RD and L2OB cell lines using MTT method. Cytotoxic effects of P. conspersa and P. perlata extracts toward three cancer cell lines were in the range from 76.33 to 163.39 µg/mL. This is the first report of the detail chemical composition of the lichens P. conspersa and P. perlata. The present study showed that tested extracts of lichens demonstrated a important antimicrobial, antioxidant and anticancer effects. That suggests that these lichens can be used as new sources of the natural antimicrobial agents, antioxidants and anticancer compounds.

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Funding

This research was supported by Ministry of Education and Science of Serbia, Projects Number: 172015.

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Authors and Affiliations

  1. Department of Pharmacy, Faculty of Medicinal Sciences, University of Kragujevac, 34000, Kragujevac, Serbia

    Nedeljko T. Manojlović, Aleksandar B. Rančić & Jovica Tomović

  2. B.C.T. consulting, Rue du 13 Aout 1956, 5000, Monastir, Tunisia

    Rachel Décor

  3. Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, 18000, Niš, Serbia

    Perica Vasiljević

Authors
  1. Nedeljko T. Manojlović
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  2. Aleksandar B. Rančić
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  3. Rachel Décor
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  4. Perica Vasiljević
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  5. Jovica Tomović
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by JT, AR and NM. The first draft of the manuscript was written by JT and NM. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jovica Tomović.

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Manojlović, N.T., Rančić, A.B., Décor, R. et al. Determination of chemical composition and antimicrobial, antioxidant and cytotoxic activities of lichens Parmelia conspersa and Parmelia perlata. Food Measure 15, 686–696 (2021). https://doi.org/10.1007/s11694-020-00672-1

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  • DOI: https://doi.org/10.1007/s11694-020-00672-1

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