Abstract
The effect of exogenous hydrogen peroxide (H2O2) on mitotic activity and chromosomal aberrations in root tip meristems of barley (Hordeum vulgare L. var. Tokak 157/37) germinated under salinity was analyzed. The inhibitory effect of salinity on mitotic index and the frequency of chromosomal aberrations increased with increasing salt concentration (0.00 control, 0.35, 0.40, 0.45 M, molal NaCl). The frequency of chromosomal aberrations of seeds germinated in medium with 0.40 M NaCl after pretreatment with H2O2 (30 μM, micromolal) was significantly higher than the control group. The highest concentration of NaCl (0.45 M) together with H2O2 caused total inhibition of germination. In this study, the intention was to determine the performance of H2O2 in alleviating detrimental effect of salt stress on mitotic activity and chromosomal aberrations. However, H2O2 did not reduce the detrimental effect of NaCl on these parameters. Also, it caused higher chromotoxic effect compared to those of control groups.
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Anuradha S, Rao SSR (2001) Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.). J Plant Growth Regul 33(2):151–153
Ashraf MY, Sarwar G, Ashraf M, Afaf R, Satar A (2002) Salinity induced changes in a-amylase activity during germination and early cotton seedling growth. Biol Plantarum 45(4):589–591
Dash M, Panda SK (2001) Salt stress induced changes in growth and enzyme activities in germinating Phaseolus muingo seeds. Biol Plantarum 44:587–589
Demidchik V, Shabala SN, Coutts KB, Tester MA, Davies JM (2003) Free oxygen radicals regulate plasma membrane Ca2+- and K+-permeable channels in plant root cell. J Cell Sci 116:81–88
Duan J, Li J, Guo S, Kang Y (2008) Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance. J Plant Physiol 165:1620–1635
Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42
Francois LE, Maas EV (1994) Crop response and management on salt-affected soils’. In: Pessaraki M (ed) Handbook of plant and crop stress, Marcel Dekker, New York, pp 149–181
Greenway H, Munns R (1980) Mechanisms of salt tolerance in non halophytes. Ann Rev Plant Physiol 31:149–190
Guan LM, Scandalios JG (2000) Hydrogen peroxide-mediated catalase gene expression in response to wounding. Free Radic Biol Med 28:1182–1190
Hameed A, Farooq S, Iqbal N, Arshad R (2004) Influence of exogenous application of hydrogen peroxide on root and seedling growth on wheat (Triticum aestivum L.). Int J Agric Biol 6(2):366–369
Hoidal JR (2001) Reactive oxygen species and cell signaling. Am J Respir Cell Mol Biol 25(6):661–663
Hossain Z, Mandal AKAM, Shukla R, Datta SK (2004) NaCl stress—its chromotoxic effects and antioxidant behavior in root of Chrysanthemum morifolium Ramat. Plant Sci 166:215–220
Kabar K (1987) Alleviation of salinity stress by plant growth regulators on seed germination. J Plant Physiol 128:179–183
Kosakivska IV (2008) Biomarkers of plants with different types of ecological strategies. Gen Appl Plant Physiol Spec Issue 34(1–2):113–126
Kovtun Y, Chiu W, Tena G, Sheen J (2000) Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plant. Proc Natl Acad Sci USA 97:2940–2945
Levine A, Tenhaken R, Dixon RA, Lamb C (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583–593
Lin CC, Kao CH (2001) Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 2:323–329
Mittler R (2002) Oxidative stress, antioxidant and stress tolerance. Trends Plant Sci 7:405–410
Mittler R, Vanderauwera S, Gollery M, van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9(10):490–498
Morita S, Kaminako H, Masumura T, Tanaka K (1999) Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress the involvement of hydrogen peroxide in oxidative stress signaling. Plant Cell Physiol 4:417–422
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Narimanow AA, Korystov YN (1997) Low dose of ionizing radiation and hydrogen peroxide stimulate plant growth. Biologia (Bratisl) 52:121–124
Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002) Hydrogen peroxide and nitric oxide as signaling molecules in plant. J Exp Bot 53:1237–1247
Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Saf 60:324–349
Patade VY, Suprasanna P, Bapat VA (2008) Effects of salt stress in relation to osmotic adjustment on sugarcane (Saccharum officinarum L.) callus cultures. Plant Growth Regul 55:169–173
Pernezny K, Nagata R, Richard N, Collins RJ, Carroll A (2001) Investigation of seed treatments for management of bacterial leaf spot of lettuce. Plant Dis 86:151–155
Prakash L, Prathapasenan G (1988) Effect of NaCl salinity and putrescine on shoot growth, tissue ion concentration and yield of rice. J Agron Crop Sci 160:325–334
Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6:65–74
Radić S, Prolić M, Pavlica M, Pevalek-Kozlina B (2005) Cytogenetic effects of osmotic stress on the root meristem cells of Centaurea ragusina L. Environ Exp Bot 54:213–218
Sharma PC, Gupta PK (1982) Karyotypes in some pulse crops. Nucleus 25:181–185
Tabur S, Demir K (2008) Tuz stresi altındaki mitotic indeks ve kromozom anormallikleri üzerine triacontanol ön uygulamasının etkileri. Biyoloji Bilimler Araştırma Dergisi (BİBAD) 1(1):11–15
Tabur S, Demir K (2009) Cytogenetic response of 24-epibrassinolide on the root meristem cells of barley seeds under salinity. Plant Growth Regul 58:119–123
Tajbakhsh M, Zhou MX, Chen ZH, Mendham NJ (2006) Physiological and cytological response of salt-tolerant and non-tolerant barley to salinity during germination and early growth. Aust J Exp Agric 46(4):555–562
Takabe T, Uchida A, Takabe T (2004) Effects of hydrogen peroxide on germination of Kentucky bluegrass. Plant biology symposium, Lake Buena Vista, FL, USA, 24–28 July 2004
Tal M (1977) Physiology of polyploid plants: DNA, RNA, protein and abscisic acid in autotetraploid and diploid tomato under low and high salinity. Bot Gaz 138(2):119–122
Vicente O, Boscaiu M, Naranjo MA, Estrelles E, Bellés JM, Soriano P (2004) Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). J Arid Environ 58:463–481
Wahid A, Perveen M, Gelani S, Basra SMA (2007) Pretreatment of seed with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. J Plant Physiol 164:283–294
Wan XY, Liu JY (2008) Comparative proteomics analysis reveals an intimate protein network provoked by hydrogen peroxide stress in rice seedling leaves. Mol Cell Proteomics 7:1469–1488
Zhang X, Dong FC, Gao JF, Song CP (2001) Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure. Cell Res 1:37–43
Zidan I, Azaizeh H, Neumann PM (1990) Dose salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification? Plant Physiol 93:7–11
Acknowledgments
The authors thank the Department of Scientific Research Project Management of Süleyman Demirel Univ. (SDUBAP) for the financial support by the project SDUBAP (1441-YL-06). Thanks also to Assist. Prof. Dr. Kürşat Çavuşoğlu (Süleyman Demirel Univ., Biology Department, Isparta, Türkiye) for his help in a preliminary study.
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Communicated by J. Sadowski.
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Cesur, A., Tabur, S. Chromotoxic effects of exogenous hydrogen peroxide (H2O2) in barley seeds exposed to salt stress. Acta Physiol Plant 33, 705–709 (2011). https://doi.org/10.1007/s11738-010-0594-7
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DOI: https://doi.org/10.1007/s11738-010-0594-7