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The smaller vesicomyid bivalves in the genus Isorropodon (Bivalvia, Vesicomyidae, Pliocardiinae) also harbour chemoautotrophic symbionts

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Abstract

Species of Isorropodon are vesicomyid bivalves for which little information is available regarding host phylogeny and bacterial symbioses. In this study we investigated the symbioses in three Isorropodon species from three cold seep areas: Isorropodon bigoti (Gulf of Guinea), Isorropodon megadesmus (Gulf of Cadiz) and Isorropodon perplexum (Eastern Mediterranean). Analysis of bacterial 16S ribosomal RNA gene sequences demonstrated that each vesicomyid species harbours a single symbiont phylotype, that symbionts from the three species cluster together, and that they are closely related to other known vesicomyid symbionts. These results are confirmed by other marker genes (encoding 23S rRNA and APS reductase) and by fluorescence in situ hybridization. Due to their extended depth range and transoceanic distribution Isorropodon species are interesting examples to further study evolutionary processes in bivalve hosts and their associated symbionts.

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Acknowledgments

Thanks are due to the chief-scientists and crew of the several cruises were specimens were collected: TTR17 Leg 2 (Gulf of Cadiz, RV Logatchev): Luis Pinheiro (Departamento de Geociências, Universidade de Aveiro) and Michael Ivanov (Moscow State University); MSM13/4 (Eastern Mediterranean; RV MS Merian, ROV Quest): Antje Boetius and Frank Wenzhofer (Max Planck Insittute); WACS (Gulf of Guinea; RV Pourquoi Pas?, ROV Victor 6000). The authors are indebted to Nelly Leger of the team AMEX (UPMC) for her help in the lab. Finally we are grateful to the two anonymous reviewers and the editor Dr. Charles Fisher for their constructive and helpful comments.

This research was supported by the European Commission Seventh Framework Programme (FP7/2007-2013) under the HERMIONE project contract 226354, French ANR DeepOases, GDRE—DIWOOD and CHEMECO ESF EURODEEP (FCT; EURODEEP/0001/2007). CFR was also supported by a post-doctoral fellowship (SFRH/BPD/64154/2009) from the Fundação para a Ciência e a Tecnologia (FCT, Portugal).

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  1. Université Pierre et Marie Curie, UMR 7138 (UPMC CNRS IRD MNHN), Systématique, Adaptation, Evolution, 7, quai St. Bernard, bâtiment A, 75005, Paris, France

    Clara F. Rodrigues & Sébastien Duperron

  2. Departamento de Biologia & CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal

    Clara F. Rodrigues & Marina R. Cunha

  3. Laboratoire Environnement Profond, Département Etudes des Ecosystèmes Profonds, Centre Ifremer de Brest, BP 71, 29280, Plouzané, France

    Karine Olu

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  1. Clara F. Rodrigues
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  4. Sébastien Duperron
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Correspondence to Clara F. Rodrigues.

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Rodrigues, C.F., Cunha, M.R., Olu, K. et al. The smaller vesicomyid bivalves in the genus Isorropodon (Bivalvia, Vesicomyidae, Pliocardiinae) also harbour chemoautotrophic symbionts. Symbiosis 56, 129–137 (2012). https://doi.org/10.1007/s13199-012-0168-0

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