Abstract
The aim of this study is to describe a new polymicrobial oral conventionalization protocol in mice. Oral biofilm samples were collected from wild C57 BL/6 mice (WG), which had not been previously submitted to any experimental procedure. The contents of these samples were used for inoculation in the oral cavity of specific pathogen free (SPF) animals. This inoculation was repeated 3 times. Qualitative cytological analyses were performed in the days 0, 16 and 80 of the experimental protocol, to check the presence or absence of microorganisms, their morphology and staining characteristics on the oral cavity of the animals. At the end of this study, was observed a combination of oral bacterial microbiota of SPF animals and wild animals in the conventionalized group (CONV). Samples collected from CONV mice on day 16, a period in which these animals had been previously inoculated 3 times with wild mouse microbiota, showed a greater amount of Gram-positive cocci, as seen in SPF animals. In addition, Gram-negative cocci were present, although in a much smaller proportion than previously seen in wild mice. On the 80th experimental day, CONV animals showed a predominance of Gram-positive cocci and bacilli. Filamentous bacteria were also seen in this group. The conventionalization of SPF animals using the technique with inoculum from the resident microbiota of wild mice proved to be an effective, low-cost and easily reproducible technique. The conventionalized animals showed the colonization of a microbiota similar to wild animals up to 80 days of experiment.
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This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.
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Conceptualization was performed by Camila Carvalho de Oliveira Coelho, Lidiany Karla Azevedo Rodrigues Gerage, Ana Paula Negreiros Nunes Alves; Fabrício Bitu Sousa, Lidiany Karla Azevedo Rodrigues Gerage, Mário Rogério Lima Mota, Ana Paula Negreiros Nunes Alves helped in methodology; Lidiany Karla Azevedo Rodrigues Gerage, Ana Paula Negreiros Nunes were involved in formal analysis and investigation; Camila Carvalho de Oliveira Coelho, Maria Imaculada de Queiroz Rodrigues wrote the original draft; Lidiany Karla Azevedo Rodrigues Gerage, Ana Paula Negreiros Nunes Alves, Camila Carvalho de Oliveira Coelho, Maria Imaculada de Queiroz Rodrigues contributed to writing—review and editing; Fabrício Bitu Sousa, Lidiany Karla Azevedo Rodrigues Gerage, Mário Rogério Lima Mota, Ana Paula Negreiros Nunes Alves supervised the study.
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This study was approved by the Ethics Committee on Animal Use of the Federal University of Ceará (protocol 85/16) and the University of Fortaleza (protocol 6466171017).
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de Queiroz Rodrigues, M.I., de Oliveira Coelho, C.C., Sousa, F.B. et al. Polymicrobial oral conventionalization model in mice. Braz J Microbiol 53, 885–890 (2022). https://doi.org/10.1007/s42770-022-00712-6
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DOI: https://doi.org/10.1007/s42770-022-00712-6