Abstract
The genus Ulkenia is characterized by the naked protoplast stage within its life cycle. However, the 18S rRNA gene tree clearly shows that this genus is not a natural taxon, because our own isolates and reported strains separately form four well-supported monophyletic groups. These four groups are clearly distinguishable by their profiles of polyunsaturated fatty acids and carotenoid pigments and cell and colony morphology, e.g., persistence of sporangial wall, manner of the cell cleavage at the zoospore formation, and development of the ectoplasmic nets. Therefore, the four groups are assigned to four genera including three new genera, i.e., Ulkenia sensu stricto, Botryochytrium, Parietichytrium, and Sicyoidochytrium gen. nov.
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References
DJ Alderman JL Harrison GB Bremer EBG Jones (1974) ArticleTitleTaxonomic revisions in the marine biflagellate fungi. The ultrastructural evidence Mar Biol 25 345–357 Occurrence Handle10.1007/BF00404978
RS Anderson BS Kraus SE McGladdery KS Reece NA Stokes (2003) ArticleTitleA thraustochytrid protist isolated from Mercenaria mercenaria: molecular characterization and host defense responses Fish Shellfish Immunol 15 183–194 Occurrence Handle12892741 Occurrence Handle10.1016/S1050-4648(02)00157-2 Occurrence Handle1:CAS:528:DC%2BD3sXlvVCqsr8%3D
G Bahnweg FK Sparrow (1974) ArticleTitleFour new species of Thraustochytrium from antarctic regions, with notes on the distribution of zoosporic fungi in the antarctic marine ecosystems Am J Bot 61 754–766 Occurrence Handle10.2307/2441791
L Bongiorni R Jain S Raghukumar RK Aggarwal (2005) ArticleTitle Thraustochytrium gaertnerium sp. nov.: a new thraustochytrid stramenopilan protist from mangroves of Goa, India Protist 156 303–315 Occurrence Handle16325543 Occurrence Handle10.1016/j.protis.2005.05.001
ML Carmona T Naganuma Y Yamanoka (2003) ArticleTitleIdentification by HPLC-MS carotenoids of the Thraustochytrium CHN-1 strain isolated from the Seto Island Sea Biosci Biotechnol Biochem 67 884–888 Occurrence Handle12784633 Occurrence Handle10.1271/bbb.67.884 Occurrence Handle1:CAS:528:DC%2BD3sXjslOmur8%3D
J Felsenstein (1981) ArticleTitleEvolutionary trees from DNA sequences: a maximum likelihood approach J Mol Evol 17 368–376 Occurrence Handle7288891 Occurrence Handle10.1007/BF01734359 Occurrence Handle1:CAS:528:DyaL3MXls1Cisr8%3D
J Felsenstein (1985) ArticleTitleConfidence limits on phylogenies: an approach using the bootstrap Evolution 39 783–791 Occurrence Handle10.2307/2408678
A Gaertner (1968) ArticleTitleEine Methode Des Quantitativen Nechweises Nieberer, Mit Pollen Koderbarer Pilze Im Meerwasser Und Im Sediment Veröff Inst Meeresforsch Bremerh Suppl 3 75–92
A Gaertner (1972) ArticleTitleCharacters used in the classification of thraustochytriaceous fungi Veröff Inst Meeresforsch Bremerh 13 183–194
A Gaertner (1977) ArticleTitleRevision of the Thraustochytriaceae (lower marine fungi) 1. Ulkenia nov. gen. with description of three new species Veröff Inst Meeresforsch Bremerh 16 139–157
M Hasegawa H Kishino T Yano (1985) ArticleTitleDating of the human-ape splitting by a molecular clock of mitochondrial DNA J Mol Evol 22 160–174 Occurrence Handle3934395 Occurrence Handle10.1007/BF02101694 Occurrence Handle1:CAS:528:DyaL2MXmtFSns7g%3D
D Honda T Yokochi T Nakahara M Erata T Higaschihara (1998) ArticleTitle Schizochytrium limacinum sp. nov., a new the thraustochytrid from a mangrove area in the west Pacific Ocean Mycol Res 102 439–448 Occurrence Handle10.1017/S0953756297005170
D Honda T Yokochi T Nakahara S Raghukumar A Nakagiri K Schaumann T Higashihara (1999) ArticleTitleMolecular phylogeny of labyrinthulids and thraustochytrids based on the sequencing of 18S ribosomal RNA gene J Eukaryot Microbiol 46 637–647 Occurrence Handle10568038 Occurrence Handle10.1111/j.1550-7408.1999.tb05141.x Occurrence Handle1:CAS:528:DyaK1MXnvVGgsb0%3D
J Huang T Aki T Yokochi T Nakahara D Honda S Kawamoto S Shigeta K Ono O Suzuki (2003) ArticleTitleGrouping the newly isolated docosahexanoic fatty acid profile and comparative analysis of 18S rRNA genes Mar Biotechnol 5 450–457 Occurrence Handle14730428 Occurrence Handle10.1007/s10126-002-0110-1 Occurrence Handle1:CAS:528:DC%2BD2cXks1Chuw%3D%3D
Y Kobayashi M Ookubo (1953) ArticleTitleStudies on the marine Phycomycetes Bull Natl Sci Mus Tokyo 33 53–65
Y Kumon R Yokoyama T Yokochi D Honda T Nakahara (2003) ArticleTitleA new labyrinthulid isolate, which solely produces n-6 docosapentaenoic acid Appl Microbiol Biotechnol 63 22–28 Occurrence Handle12750856 Occurrence Handle10.1007/s00253-003-1333-4 Occurrence Handle1:CAS:528:DC%2BD3sXosVajt74%3D
Y Kumon R Yokoyama Z Haque T Yokochi D Honda T Nakahara (2006) ArticleTitleA new labyrinthulid isolate that produces only docosahexaenoic acid Mar Biotechnol 8 170–177 Occurrence Handle16380808 Occurrence Handle10.1007/s10126-005-5098-x Occurrence Handle1:CAS:528:DC%2BD28Xjs1Kksb4%3D
CA Leander D Porter (2000) ArticleTitleRedefining the genus Aplanochytrium (phylum Labyrinthulomycota) Mycotaxon 76 439–444
CA Leander D Porter (2001) ArticleTitleThe Labyrinthulomycota is comprised of three distinct lineages Mycologia 93 459–464 Occurrence Handle10.2307/3761732
CA Leander D Porter BS Leander (2004) ArticleTitleComparative morphology and molecular phylogeny of aplanochytrids (Labyrinthulomycota) Eur J Protistol 40 317–328 Occurrence Handle10.1016/j.ejop.2004.07.003
DD Leipe PO Wainright JH Gunderson D Porter DJ Patterson F Valois S Himmerich ML Sogin (1994) ArticleTitleThe stramenopiles from a molecular perspective: 16S-like rRNA sequences from Labyrinthuloides minuta and Cafeteria roenbergensis Phycologia 33 369–377
I Moro E Negrisolo A Callegaro C Andreoli (2003) ArticleTitle Aplanochytrium stocchinoi, a new Labyrinthulomycota from the Southern Ocean (Ross Sea, Antarctica) Protist 154 331–340 Occurrence Handle14658493 Occurrence Handle10.1078/143446103322454103 Occurrence Handle1:CAS:528:DC%2BD2cXkvFensQ%3D%3D
MG Murray WF Thompson (1980) ArticleTitleRapid isolation of high molecular weight plant DNA Nucleic Acids Res 8 4321–4325 Occurrence Handle7433111 Occurrence Handle10.1093/nar/8.19.4321 Occurrence Handle1:CAS:528:DyaL3cXmtVSmtL8%3D
T Nakahara T Yokochi T Higashihara S Tanaka T Yaguchi D Honda (1996) ArticleTitleProduction of docosahexaenoic and docosapentaenoic acid by Schizochytrium sp. isolated from Yap Island J Am Oil Chem Soc 73 1421–1426 Occurrence Handle10.1007/BF02523506 Occurrence Handle1:CAS:528:DyaK28XntFOhsbs%3D
M Nakayama S Watanabe K Mitsui H Uchida I Inoue (1996) ArticleTitleThe phylogenetic relationship between the Chlamydomonadales and Chlorococcales inferred from 18S rDNA sequences data Phycol Res 44 47–55 Occurrence Handle10.1111/j.1440-1835.1996.tb00037.x Occurrence Handle1:CAS:528:DyaK28Xkt1KhsLc%3D
D Porter (1989) Phylum Labyrinthulomycota L Margulis JO Corlis M Melconian DJ Chapman (Eds) Handbook of Protoctista Jones and Bartlett Boston 388–398
S Raghukumar (1977) ArticleTitleA new species of the genus Ulkenia Gaertner (lower marine fungi) from the North Sea Veröff Inst Meeresforsch Bremerh 16 159–165
S Raghukumar (1982a) ArticleTitleFine structure of the thraustochytrid Ulkenia amoeboidea. I. Vegetative thallus and formation of the amoeboid stage Can J Bot 60 1092–1102
S Raghukumar (1982b) ArticleTitleFine structure of the thraustochytrid Ulkenia amoeboidea. II. The amoeboid stage and formation of zoospores Can J Bot 60 1103–1114
S Raghukumar (1988) ArticleTitle Schizochytrium mangrovei sp. nov., a thraustochytrid from mangroves in India Trans Br Mycol Soc 90 627–631 Occurrence Handle10.1016/S0007-1536(88)80068-8
S Raghukumar (1992) ArticleTitleBacterivory: a novel dual role for thraustochytrids in the sea Mar Biol 113 165–169 Occurrence Handle10.1007/BF00367650
A Rzhetsky M Nei (1992) ArticleTitleA simple method for estimating and testing minimum-evolution trees Mol Biol Evol 9 945–967 Occurrence Handle1:CAS:528:DyaK38Xls1KhsL0%3D
A Rzhetsky M Nei (1993) ArticleTitleTheoretical foundation of the minimum-evolution method of phylogenetic inference Mol Biol Evol 10 1073–1095 Occurrence Handle8412650 Occurrence Handle1:STN:280:DyaK2c%2FhvVGqsg%3D%3D
S Shimizu H Kawashima Y Shinmen K Akimoto H Yamada (1988) ArticleTitleProduction of eicosapentaenoic acid by Mortierella fungi J Am Oil Chem Soc 65 1455–1459 Occurrence Handle1:CAS:528:DyaL1cXlvVylu78%3D
Sparrow FK (1943) The aquatic Phycomycetes, 1st edn. Exclusive of the Saprolegniales and Pythium. University of Michigan Press, Ann Arbor
FK Sparrow (1960) The aquatic Phycomycetes EditionNumber2nd edn University of Michigan Press Ann Arbor
NA Stokes LM Ragone Calvo KS Reece EM Burreson (2002) ArticleTitleMolecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria Dis Aquat Org 52 233–247 Occurrence Handle12553451 Occurrence Handle10.3354/dao052233 Occurrence Handle1:CAS:528:DC%2BD3sXhslKgsbY%3D
DL Swofford (2003) PAUP*: phylogenetic analysis using parsimony (* and other methods), version 4.0b 10 Sinauer Associates Sunderland, MA
Y Takao Y Tomaru K Nagasaki Y Sasakura R Yokoyama D Honda (2007) ArticleTitleFluorescence in situ hybridization using 18S rRNA-targeted probe for specific detection of thraustochytrids (Labyrinthulomycetes) Plankton Benthos Res 2 91–97 Occurrence Handle10.3800/pbr.2.91
JD Thompson DC Higgins TJ Gibson (1994) ArticleTitleClustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position, specific gap penalties and weight matrix choice Nucleic Acids Res 22 4673–4680 Occurrence Handle7984417 Occurrence Handle10.1093/nar/22.22.4673 Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D
Y Yokochi D Honda T Higashihara T Nakahara (1998) ArticleTitleOptimization of docosahexaenoic acid production by Schizochytrium limacinum SR21 Appl Microbiol Biotechnol 49 72–76 Occurrence Handle10.1007/s002530051139 Occurrence Handle1:CAS:528:DyaK1cXhtVantbc%3D
R Yokoyama D Honda (2007) ArticleTitleTaxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomical characteristics and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes, stramenopiles): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov Mycoscience 48 199–211 Occurrence Handle10.1007/s10267-006-0362-0 Occurrence Handle1:CAS:528:DC%2BD2sXht1ahsbfL
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Yokoyama, R., Salleh, B. & Honda, D. Taxonomic rearrangement of the genus Ulkenia sensu lato based on morphology, chemotaxonomical characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Ulkenia and erection of Botryochytrium, Parietichytrium, and Sicyoidochytrium gen. nov.. Mycoscience 48, 329–341 (2007). https://doi.org/10.1007/s10267-007-0377-1
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DOI: https://doi.org/10.1007/s10267-007-0377-1