Abstract
Genetic engineering using clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas9) has revolutionized biomedical science, uncovering the function of genes involved in health and disease. C. elegans is a powerful genetic model that allows us to understand the impact of altered genes from the molecular level to behavior.
Here, I describe a protocol to knock out genes in C. elegans by employing multiple sgRNAs combined with the homologous recombination-mediated repair.
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Acknowledgments
Thank you to Dr. Erik Jorgensen (UU, HHMI) for hosting J.L.T.A. during the Fulbright program 2018–2019. This research was funded by an NIGMS grant to EMJ R01 GM095817. José Luis Téllez-Arreola is a grantee of Fulbright-Garcia Robles para investigadores de COMEXUS.
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Téllez-Arreola, J.L. (2021). Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-Mediated Repair. In: Islam, M.T., Molla, K.A. (eds) CRISPR-Cas Methods. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1657-4_23
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DOI: https://doi.org/10.1007/978-1-0716-1657-4_23
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