Skip to main content
SpringerLink
Log in

Hemagglutination (Inhibition) Assay

  • Protocol
  • First Online:
Lectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1200))

Abstract

The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension. By serially diluting the lectin in a 96-well microtiter plate and adding a constant quantity of erythrocytes, the lectin activity can be estimated.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Stillmark PH (1888) Über Ricin, ein giftiges Ferment aus den Samen von Ricinus comm. L. und einigen anderen Euphorbiaceen. Kaiserliche Universität zu Dorpat, Tartu, Estonia

    Google Scholar 

  2. Sumner JB (1919) The globulins of the jack bean, Canavalia ensiformis. J Biol Chem 37:137–142

    CAS  Google Scholar 

  3. Sumner JB, Howell SF (1936) Identification of hemagglutinin of Jack bean with concanavalin A. J Bacteriol 32:227–237

    PubMed  CAS  PubMed Central  Google Scholar 

  4. Landsteiner K, Raubitschek H (1907) Beobachtungen über Hämolyse und Hämagglutination. Zbl Bakt I Abt Orig 45:600–607

    Google Scholar 

  5. Kawsar SM, Matsumoto R, Fujii Y, Yasumitsu H, Dogasaki C, Hosono M, Nitta K, Hamako J, Matsui T, Kojima N, Ozeki Y (2009) Purification and biochemical characterization of a D-galactose binding lectin from Japanese sea hare (Aplysia kurodai) eggs. Biochemistry (Mosc) 74:709–716

    Article  CAS  Google Scholar 

  6. Ueno M, Ogawa H, Matsumoto I, Seno N (1991) A novel mannose-specific and sugar specifically aggregatable lectin from the bark of the Japanese pagoda tree (Sophora japonica). J Biol Chem 266:3146–3153

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Graduate School of Humanities and Sciences and Glycoscience Institute, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo, 112-8610, Japan

    Kotone Sano & Haruko Ogawa

Authors
  1. Kotone Sano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haruko Ogawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Haruko Ogawa .

Editor information

Editors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

    Jun Hirabayashi

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Sano, K., Ogawa, H. (2014). Hemagglutination (Inhibition) Assay. In: Hirabayashi, J. (eds) Lectins. Methods in Molecular Biology, vol 1200. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1292-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-1292-6_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1291-9

  • Online ISBN: 978-1-4939-1292-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation