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Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation

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Clathrin-Mediated Endocytosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1847))

Abstract

More than a trillion nerve terminals interconnect neurons in the human brain. These terminals are fundamental for signal transmission and nerve cell communication. Among other techniques, the isolation of nerve terminals [or synaptosomes (Whittaker et al. Biochem J, 90(2):293–303, 1964)] has been fundamental to study the biochemistry and the physiology of the nervous system. This chapter describes the isolation and purification of intact synaptosomes from rodent brain tissue that can be used to further characterize synaptic structure and function and to examine the molecular mechanisms of neurotransmission.

The original version of this chapter was revised. A correction to this chapter can be found at https://doi.org/10.1007/978-1-4939-8719-1_19

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Change history

  • 24 October 2018

    This book was inadvertently published with the incorrect title as Clathrin-Mediated Endoytosis: Methods and Protocols. This has now been corrected throughout the book to Clathrin-Mediated Endocytosis: Methods and Protocols.

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Authors and Affiliations

  1. Department of Neuroscience and Cell Biology, HHMI, Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT, USA

    Mirko Messa

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  1. Mirko Messa
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Correspondence to Mirko Messa .

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Editors and Affiliations

  1. Department of Cellular & Molecular Physiology, University of Liverpool, Institute of Translational Medicine, Liverpool, UK

    Laura E. Swan

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Messa, M. (2018). Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation. In: Swan, L. (eds) Clathrin-Mediated Endocytosis. Methods in Molecular Biology, vol 1847. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8719-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-8719-1_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8717-7

  • Online ISBN: 978-1-4939-8719-1

  • eBook Packages: Springer Protocols

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