Issue 61, 2019
From the journal:

RSC Advances

Clickable poly-l-lysine for the formation of biorecognition surfaces†

Daniele Di Iorio, ORCID logo a   Almudena Marti,a   Sander Koemana  and  Jurriaan Huskens ORCID logo *a  

* Corresponding authors

a Molecular NanoFabrication Group, MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands
E-mail: j.huskens@utwente.nl

Abstract

Biomolecules are immobilized onto surfaces employing the fast and stable adsorption of poly-L-lysine (PLL) polymers and the versatile copper-free click chemistry reactions. This method provides the combined advantages of versatile surface adsorption with density control using polyelectrolytes and of the covalent and orthogonal immobilization of biomolecules with higher reaction rates and improved yields of click chemistry. Using DNA attachment as a proof of concept, control over the DNA probe density and applicability in electrochemical detection are presented.

Graphical abstract: Clickable poly-l-lysine for the formation of biorecognition surfaces

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C9RA08714A
Article type
Paper
Submitted
17 Sep 2019
Accepted
24 Oct 2019
First published
04 Nov 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 35608-35613

Permissions

Request permissions

Clickable poly-L-lysine for the formation of biorecognition surfaces

D. Di Iorio, A. Marti, S. Koeman and J. Huskens, RSC Adv., 2019, 9, 35608 DOI: 10.1039/C9RA08714A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements