Table of Contents
German Programme on Biodiversity and Global Change
(Phase I, 2000 - 2004) Funded by BMBF
Status Report 2001
INTRODUCTION TO THE PROGRAMME BIOLOG.................................... 10
BIOLOG EUROPE.........................................................................................................................12
BIOLOG AFRICA..........................................................................................................................14
BIODIVERSITY INFORMATICS IN THE BIOLOG PROGRAMME .........................................16
BIOLOG EUROPE
Project ID: 01LC0002.....................................................................................................................20
GLOBAL CHANGE-DEPENDENT SPREAD OF NEOPHYTES: RELATIONSHIP TO ECOSYSTEM
CHANGE (CASE STUDY: FLOODPLAINS OF THE RIVER RHINE)
Project ID: 01LC0004.....................................................................................................................22
RECONSTRUCTING HISTORICAL BIODIVERSITY FROM ARCHIVAL SOURCES
Project ID: 01LC0005.....................................................................................................................24
BIODIVERSITY CHANGE DURING 20 YEARS OF SUCCESSION: IMPLICATIONS FOR MANAGEMENT
OF RESTORATION
Project ID: 01LC0006.....................................................................................................................26
ECOLOGY AND BIODIVERSITY OF HIGHER FUNGI IN CENTRAL EUROPE (GERMANY)
Project ID: 01LC0010.....................................................................................................................28
CONDITIONS OF A RATIONAL BIODIVERSITY POLICY FOR GERMANY, AS A PART OF THE
EUROPEAN UNION
Project ID: 01LC0012 (Subproject 1)..............................................................................................30
EVOLUTIONARY CONSEQUENCES OF BIOLOGICAL INVASIONS – INTROGRESSIVE
HYBRIDIZATION BETWEEN INVASIVE AND NATIVE RORIPPA SPECIES (BRASSICACEAE).
Project ID: 01LC0012 (Subproject 2)..............................................................................................32
EVOLUTIONARY CONSEQUENCES OF BIOLOGICAL INVASIONS – ONGOING HYBRIDIZATION
PROCESSES BETWEEN CARDAMINE TAXA AT THE URNERBODEN, CENTRAL SWITZERLAND
1
Project ID: 01LC0013.....................................................................................................................34
BIODIVERSITY AND ECOSYSTEM FUNCTION IN GRASSLANDS - THE EFFECTS OF INSECT
HERBIVORY AND DROUGHT STRESS
Project ID: 01LC0013 (Subproject STOFF)....................................................................................36
THE CARBON AND NITROGEN CYCLES IN GRASSLAND ECOSYSTEMS OF DIFFERENT
DIVERSITIES
Project ID: 01LC0013 (Subproject RAUM)....................................................................................38
A SPATIAL MODEL FOR INDICATING ECOSYSTEM FUNCTIONS
Project ID: 01LC0013 (Subproject ENTO).....................................................................................40
THE RELATIONSHIP BETWEEN INSECT HERBIVORIE, PLANT DIVERSITY, INSECT DIVERSITY AND
ECOSYSTEM PROCESSES
Project ID: 01LC0013 (Subproject GENMOD) ..............................................................................42
GENETIC AND PHENOTYPIC DIVERSITY AND RESPONSE TO DISTURBANCES IN GRASSLAND
SYSTEMS: MODELLING AND EMPIRICAL RESEARCH
Projekt ID: 01LC0014.....................................................................................................................44
BIODIVERSITY AND ECOSYSTEM FUNCTIONS IN GRASSLAND
Projekt ID: 01LC0014 (Subproject Vegetation) ..............................................................................46
TAXONOMIC, FUNCTIONAL AND STRUCTURAL CHARACTERISTICS OF ABOVE-GROUND PLANT
FEATURES AND THEIR INFLUENCE ON ECOSYSTEM FUNCTIONS
Projekt ID: 01LC0014 (Subproject Roots) ......................................................................................48
THE ROLE OF BIODIVERSITY FOR STORAGE OF CARBON AND MINERALS IN ROOTS, AND
ACQUISITION OF SOIL RESSOURCES IN GRASSLAND
Projekt ID: 01LC0014 (Subproject Soil) .........................................................................................50
THE IMPACT OF PLANT DIVERSITY OF GRASSLANDS ON WATER- AND NUTRIENT-FLUXES IN
SOILS AND SOIL ORGANIC MATTER
Project ID: 01LC0015 (Subproject VEGETATION ECOLOGY) ...................................................52
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE 'VORPOMMERSCHE
BODDENLANDSCHAFT' – SPATIO-TEMPORAL DYNAMICS AND ECOSYSTEM FUNCTION OF
BIODIVERSITY IN BRACKISH GRASSLANDS
PROJECT ID: 01LC0015 (Subproject LANDSCAPE ECONOMY)...............................................54
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE 'VORPOMMERSCHE
BODDENLANDSCHAFT' – ECONOMICAL VALUATION OF BIODIVERSITY
Project ID: 01LC0015 (Subproject ANIMAL ECOLOGY).............................................................56
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE 'VORPOMMERSCHE
BODDENLANDSCHAFT' - DYNAMICS IN DIVERSITY OF ANIMAL SPECIES AND COMMUNITIES IN A
CHANGING ENVIRONMENT WITH SPECIAL REGARD TO DISPERSAL PROCESSES
Project ID: 01LC0015 (Subproject SOIL SCIENCE) .....................................................................58
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE 'VORPOMMERSCHE
BODDENLANDSCHAFT' - WATER AND SALT DYNAMICS OF SALTY MEADOW AND MARSH SOILS
Project ID: 01LC0018 (SUBICON Subproject 1)............................................................................60
SUBICON - SUCCESSIONAL CHANGE AND BIODIVERSITY CONSERVATION IN A RED OAK
CHRONOSEQUENCE –
SUCCESSION OF DIVERSITY AND FUNCTION OF ECTOMYCORRHIZAL TYPES IN RED OAK FOREST
ECOSYSTEMS
Project ID: 01LC0018 (SUBICON Subproject 2)............................................................................62
BIODIVERSITY OF PLANT COMMUNITIES AND SELECTED PHYTOPHAGES IN RED OAK
DOMINATED ECOSYSTEMS
2
Project ID: 01LC0018 (SUBICON Subproject 3)............................................................................64
GENETIC DIVERSITY OF SELECTED PLANTS, PHYTOPHAGES AND ZOOPHAGES IN SUCCESSIONAL
RED OAK ECOSYSTEMS
Project ID: 01LC0018 (SUBICON Subproject 4)............................................................................66
STRUCTURE OF THE PREDATOR COMMUNITY IN A RED OAK CHRONOSEQUENCE
Project ID: 01LC0020 (BIOPLEX Subproject 1) ............................................................................68
BIODIVERSITY AND SPATIAL COMPLEXITY IN AGRICULTURAL LANDSCAPES UNDER GLOBAL
CHANGE (BIOPLEX) - MODELLING AND GEOSTATISTICS
Project ID: 01LC0020 (BIOPLEX Subproject 2) ............................................................................70
BIODIVERSITY AND SPATIAL COMPLEXITY IN AGRICULTURAL LANDSCAPES UNDER GLOBAL
CHANGE (BIOPLEX) - AGROECOLOGY
Project ID: 01LC0020 (BIOPLEX Subproject 3) ............................................................................72
BIODIVERSITY AND SPATIAL COMPLEXITY IN AGRICULTURAL LANDSCAPES UNDER GLOBAL
CHANGE (BIOPLEX) - PHYTOPATHOLOGY
Project ID: 01LC0020 (BIOPLEX Subproject 4) ............................................................................74
BIODIVERSITY AND SPATIAL COMPLEXITY IN AGRICULTURAL LANDSCAPES UNDER GLOBAL
CHANGE (BIOPLEX) - Soil BIOTA
Project ID: 01LC0020 (BIOPLEX Subproject 5) ............................................................................76
BIODIVERSITY AND SPATIAL COMPLEXITY IN AGRICULTURAL LANDSCAPES UNDER GLOBAL
CHANGE (BIOPLEX) - SOCIAL-ECONOMIC EVALUATION AND APPRAISAL WITH RESPECT TO
BIODIVERSITY IN AGRARIAN LANDSCAPES
Project ID: 01LC0021 (Subproject 1)..............................................................................................78
BIODIVERSITY OF NITROGEN-FIXING MICROORGANISMS
Project ID: 01LC0021 (Subproject 2)..............................................................................................80
BIODIVERSITY OF NITRIFYING AND SULFATE-REDUCING PROKARYOTES
Project ID: 01LC0021 (Subproject 3)..............................................................................................82
BIODIVERSITY OF METHANOTROPHIC AND NITRATE-REDUCING BACTERIA
Project ID: 01LC0021 (Subproject 4)..............................................................................................84
BIODIVERSITY AND SUCCESSIONS IN TEMPORARY TERRESTRIAL-AQUATIC INTERFACES
Project ID: 01LC0021 (Subproject 5)..............................................................................................86
INVESTIGATION OF PROKARYOTIC DIVERSITY BY OPTIMIZED CULTIVATION METHODS
Project ID: 01LC0021 (Subproject 6)..............................................................................................88
THE ROLE OF N-ACYL-L-HOMOSERINE LACTONE (AHL) MEDIATED COMMUNICATION FOR THE
STRUCTURE AND PERSISTENCE OF MICROBIAL RHIZOSPHERE COMMUNITIES
Project ID: 01LC0021 (Subproject 7)..............................................................................................90
BIODIVERSITY OF PROTOZOA IN THE RHIZOSPHERE OF PLANTS: EFFECTS ON ACTIVITY,
PERFORMANCE AND DIVERSITY OF MICROORGANISMS AND ON PLANT GROWTH.
Project ID: 01LC0101 (Subproject 1)..............................................................................................92
AN ANALYSIS OF PREVAILING ECONOMIC AND POLITICAL STRATEGIES FOR THE PROTECTION
OF BIODIVERSITY - THE TRADE IN GENETIC RESSOURCES: A VIABLE CONTRIBUTION TO THE
PROTECTION OF BIODIVERSITY?
Project ID: 01LC0101 (Subproject 2)..............................................................................................94
AN ANALYSIS OF PREVAILING ECONOMIC AND POLITICAL STRATEGIES FOR THE PROTECTION
OF BIODIVERSITY - THE PROTECTION OF BIODIVERSITY: NEW APPROACHES AND INSTRUMENTS
IN INTERNATIONAL LAW
3
BIOLOG AFRICA
Project ID: 01LC0024 (BIOTA Southern Africa) ............................................................................98
A RESEARCH PROJECT MONITORING AND ANALYSING BIODIVERSITY AND ITS CHANGE ALONG
A TRANSECT IN NAMIBIA AND SOUTH AFRICA
Project ID: 01LC0024 (BIOTA Southern Africa S01)................................................................... 100
REMOTE SENSING AND GIS BASED SURVEY OF SPATIAL AND TEMPORAL BIODIVERSITY
DYNAMICS AND ANALYSIS OF BIODIVERSITY AND GEODIVERSITY INTERRELATIONSHIPS
Project ID: 01LC0024 (BIOTA Southern Africa S02)................................................................... 102
EDAPHICAL DIVERSITY AND BIODIVERSITY IN MUTUAL DEPENDENCY
Project ID: 01LC0024 (BIOTA Southern Africa S03)................................................................... 104
BIODIVERSITY OF SOUTHWESTERN AFRICAN FUNGI: INTERACTIONS WITH HIGHER PLANTS,
FUNCTIONAL DIVERSITY AND DNA-TAXONOMY
Project ID: 01LC0024 (BIOTA Southern Africa S04)................................................................... 106
DELVELOPMENT OF AN INTEGRATED SYSTEM FOR THE INPUT, STORAGE AND ANALYSIS OF
BIODIVERSITY DATA IN SOUTHERN AFRICA
Project ID: 01LC0024 (BIOTA Southern Africa S05)................................................................... 108
BIOLOGICAL SOIL CRUSTS OF TERRESTRIAL ECOSYSTEMS: MODES OF PATTERN FORMATION
AND THEIR MODELLING CONCERNING DIVERSITY, STRUCTURE AND FUNCTION IN
CRYPTOGAMIC COMMUNITIES
Project ID: 01LC0024 (BIOTA Southern Africa S06)................................................................... 110
CHANGES IN BOTANICAL BIODIVERSITY WITH REGARD TO CHANGES IN LAND USE PRACTICES
AND CLIMATE: STANDARDISED MONITORING AND TRANSECT ANALYSIS
Project ID: 01LC0024 (BIOTA Southern Africa S07)................................................................... 112
FUNCTIONAL ZOODIVERSITY IN SOUTHERN AFRICA UNDER CHANGING ENVIRONMENTS AND
HUMAN USE
Project ID: 01LC0024 (BIOTA Southern Africa S08)................................................................... 114
DIVERSITY OF NAMIBIAN DRAGONFLIES: EFFECTS OF ANTHROPOGENIC CHANGES AND
MODELLING ON DIFFERENT SCALES
Project ID: 01LC0024 (BIOTA Southern Africa S09)................................................................... 116
STRUCTURAL AND SPECIES DIVERSITY IN SEMI-ARID SAVANNAHS OF SOUTHERN AFRICA: THE
IMPACT OF LAND USE AND CLIMATIC CHANGE
Project ID: 01LC0024 (BIOTA Southern Africa S10)................................................................... 118
WHICH PROCESSES AND MECHANISMS DETERMINE THE BIODIVERSITY IN (SEMI-) ARID PLANT
COMMUNITIES?
Project ID: 01LC0024 (BIOTA Southern Africa S11)................................................................... 120
SOCIO-ECONOMIC ASPECTS OF CHANGES IN BIODIVERSITY IN SOUTHERN AFRICA
Project ID: 01LC0007................................................................................................................... 122
BIOLOGICAL STUDIES ON MYCO-HETEROTROPHIC PLANTS WITH SPECIAL EMPHASIS OF THEIR
MYCORRHIZAE
Project ID: 01LC0017 (BIOTA West Africa) ................................................................................ 124
BIOTA WEST AFRICA – GENERAL SUMMARY
Project ID: 01LC0017 (BIOTA West Africa W01)........................................................................ 128
REMOTE SENSING AND GIS BASED SURVEY OF SPATIAL AND TEMPORAL BIODIVERSITY
DYNAMICS AND ANALYSIS OF BIODIVERSITY AND GEODIVERSITY INTERRELATIONSHIPS
4
Project ID: 01LC0017 (BIOTA West Africa W02)........................................................................ 130
BIOPHYSICAL AND HYDROMETEOROLOGICAL PARAMETERS WITHIN THE FRAMEWORK OF
TERRESTRIAL BIODIVERSITY RESEARCH
Project ID: 01LC0017 (BIOTA West Africa W03)........................................................................ 132
BIODIVERSITY OF AFRICA IN THE GLOBAL CONTEXT: SPATIAL PATTERNS OF VASCULAR PLANT
DIVERSITY IN A CHANGING ENVIRONMENT (BIOMAPS PROJECT)
Project ID: 01LC0017 (BIOTA West Africa W04)........................................................................ 134
PHYTODIVERSITY AND DYNAMICS OF HABITAT FRAGMENTS IN THE IVORY COAST: SPATIAL
AND TEMPORAL PATTERNS OF BIODIVERSITY CHANGES, EDGE EFFECTS, AND THEIR
FUNCTIONAL CONSEQUENCES IN NATURAL AND DISTURBED ECOSYSTEM MOSAICS
Project ID: 01LC0017 (BIOTA West Africa W06)........................................................................ 136
DETERMINANTS OF SMALL-SCALE MOSAICS OF ARTHROPOD COMMUNITIES IN NATURAL AND
ANTHROPOGENICALLY DISTURBED HABITATS
Project ID: 01LC0017 (BIOTA West Africa W07)........................................................................ 140
COMPARATIVE STUDIES ON EPHEMERAL SAVANNA WATERS AND THEIR SIGNIFICANCE FOR
DIVERSITY AND PRODUCTIVITY OF FISH COMMUNITIES IN NATURAL AND
ANTHROPOGENICALLY DISTURBED RIVERS
Project ID: 01LC0017 (BIOTA West Africa W08)........................................................................ 142
AMPHIBIAN DIVERSITY AND DIVERSITY CHANGES IN WEST AFRICA: GLOBAL, REGIONAL OR
LOCAL CAUSES?
Project ID: 01LC0017 (BIOTA West Africa W09)........................................................................ 144
THE INFLUENCE OF LOCAL AND REGIONAL FACTORS ON THE DIVERSITY, STRUCTURE, AND
FUNCTION OF WEST AFRICAN BAT COMMUNITIES (CHIROPTERA)
Project ID: 01LC0017 (BIOTA West Africa W11)........................................................................ 146
PHYTODIVERSITY IN THE WEST AFRICAN SAHEL AND SUDAN ZONE – DEVELOPMENT AND
EVALUATION
Project ID: 01LC0022................................................................................................................... 148
THE CUVETTE CENTRALE AS RESERVOIR OF MEDICINAL PLANTS
Project ID: 01LC0025 (BIOTA East Africa E01) .......................................................................... 150
BIOTA EAST AFRICA: BIODIVERSITY IN CONVERSION – THE INFLUENCE OF FRAGMENTATION
AND MAN-MADE EXPLOITATION ON THE BIODIVERSITY OF TROPICAL MONTANE FORESTS
Project ID: 01LC0025 (BIOTA East Africa E02) .......................................................................... 152
IMPACT OF BIODIVERSITY CHANGE ON THE EXCHANGE POTENTIAL OF TRACE GASES BETWEEN
ECOSYSTEMS AND ATMOSPHERE
Project ID: 01LC0025 (BIOTA East Africa E03) .......................................................................... 154
REGENERATION OF TROPICAL MONTANE TREE SPECIES – SPATIAL AND TEMPORAL DYNAMICS
OF FEED BACK PROCESSES
Project ID: 01LC0025 (BIOTA East Africa E04) .......................................................................... 156
INFLUENCE OF NATURAL AND ANTHROPOGENIC FRAGMENTATION ON THE VEGETATION IN
MONTANE RAINFORESTS OF KAKAMEGA AND MT. KENYA
Project ID: 01LC0025 (BIOTA East Africa E06) .......................................................................... 158
LEPIDOPTERA AS INDICATORS OF HUMAN IMPACT ON TROPICAL RAINFOREST ECOSYSTEMS IN
EASTERN AFRICA
Project ID: 01LC0025 (BIOTA East Africa E07) .......................................................................... 160
DIVERSITY AND SPECIES COMPOSITION OF ODONATA AS INDICATORS OF BIOTOPE QUALITY OF
EAST AFRICAN RAIN FORESTS AND THEIR REPLACEMENT COMMUNITIES
5
Project ID: 01LC0025 (BIOTA East Africa E08) .......................................................................... 162
MEASURING AND MONITORING AMPHIBIAN DIVERSITY IN EAST AFRICA: SEPARATING GLOBAL
FROM REGIONAL AND LOCAL CAUSES
Project ID: 01LC0025 (BIOTA East Africa E09) .......................................................................... 164
THE INFLUENCE OF LAND USE MODES ON DIVERSITY, ABUNDANCE AND GUILD STRUCTURE OF
COPROPHAGOUS BEETLES IN THE AFRICAN FOREST-SAVANNA-MOSAIC
Project ID: 01LC0025 (BIOTA East Africa E10) .......................................................................... 166
EFFECTS OF HABITAT FRAGMENTATION ON PLANT-POLLINATOR INTERACTIONS IN EAST
AFRICAN RAINFORESTS
Project ID: 01LC0025 (BIOTA East Africa E11) .......................................................................... 168
EFFECT OF FRAGMENTATION ON SEED DISPERSAL, REGENERATION AND DYNAMICS OF
KAKAMEGA FOREST, KENYA
Project ID: 01LC0025 (BIOTA East Africa E12) .......................................................................... 170
MONTANE FOREST ECOSYSTEMS IN KENYA - DIVERSITY AND POTENTIAL FOR USE
Project ID: 01LC0025 (BIOTA East Africa, Yemen E13-E16)...................................................... 172
BIOTA-EAST YEMEN-PROJECT-GROUP: BIODIVERSITY AND ECOLOGY OF THE PALAEO-AFRICAN
REFUGE AREAS IN SOUTHERN ARABIA AND ON SOCOTRA (REPUBLIC OF YEMEN)
Project ID: 01LC0025 (BIOTA East Africa, Yemen E13) ............................................................. 174
PHYTODIVERSITY AND VEGETATION TYPES OF REPRESENTATIVE PALEO-AFRICAN REFUGIA IN
THE COASTAL MOUNTAINS OF SOUTHERN YEMEN AND ON SOCOTRA (REPUBLIC OF YEMEN)
Project ID: 01LC0025 (BIOTA East Africa, Yemen E14) ............................................................. 176
ECOLOGICAL CAUSES OF BIODIVERSITY IN THE COASTAL MOUNTAINS OF SOUTHERN YEMEN
AND ON SOCOTRA (REPUBLIC OF YEMEN)
Project ID: 01LC0025 (BIOTA East Africa, Yemen E15) ............................................................. 178
PHYLOGEOGRAPHIC AND POPULATION GENETIC ASPECTS OF THE PHYTODIVERSITY IN
PALAEO-AFRICAN REFUGIA OF THE COASTAL MOUNTAINS OF SOUTHERN YEMEN AND
SOCOTRA (REPUBLIC OF YEMEN)
Project ID: 01LC0025 (BIOTA East Africa, Yemen E16) ............................................................. 180
EVOLUTIONARY HISTORY AND PHYLOGEOGRAPHY OF SOUTH ARABIAN FAUNAL ELEMENTS
Project ID: 01LC0001................................................................................................................... 182
BIODIVERSITY OF SELECTED MODEL GROUPS OF THE PHYTOPLANKTON IN STANDING INLAND
WATERS OF KENYA
BIODIVERSITY INFORMATICS
Project ID: 01LC9901 (SysTax) .................................................................................................... 186
SYSTAX - ELECTRONIC DATA PROCESSING FOR RECORDING AND ANALYSING BIODIVERSITY
DATA WITH THE SYSTEMATIC AND TAXONOMIC DATABASE SYSTEM SYSTAX
Project ID: 01LC9902 (ARB)........................................................................................................ 188
AUTOMATED MAINTAINANCE OF INTEGRATED DATABASES FOR RRNA AND OTHER RELEVANT
GENE SEQUENCES; SOFTWARE DEVELOPMENT, OPTIMIZATION, AND PARALLELIZATION FOR
AUTOMATED SEARCHING OF SEQUENCE IDENTIFIERS FOR ANALYZING DIVERSITY
Project ID: 01LC9903 (ZEFOD)................................................................................................... 190
REGISTER FOR BIOLOGICAL RESEARCH COLLECTIONS IN GERMANY
Project ID: 01LC9904 (EDIS) ....................................................................................................... 192
EDIS – ENTOMOLOGICAL DATA INFORMATION SYSTEM
6
Project ID: 01LC9904 (EDIS, Subproject ABIS) .......................................................................... 194
ABIS - AUTOMATED IDENTIFICATION OF BEE SPECIES
Project ID: 01LC9904 (EDIS, Subproject DNATAX 1)................................................................. 196
DNATAX – DNA TAXONOMY AS A TOOL IN BIODIVERSITY RESEARCH: RECORDING,
ASSESSMENT, MONITORING AND UTILIZATION OF THE GENETIC DIVERSITY OF EUKARYONT
SYSTEMS
Project ID: 01LC9904 (EDIS, Subproject DNATAX 2 - MOLART)............................................. 198
MOLART (DNATAX 2): MOLECULAR IDENTIFICATION OF COLLEMBOLA: METHOD OPTIMISATION
VERIFICATION - DOCUMENTATION
Project ID: 01LC9904 (EDIS, Subproject DORSA) ...................................................................... 200
DORSA - GERMAN ORTHOPTERA COLLECTIONS
Project ID: 01LC9904 (EDIS, Subproject GART) ........................................................................ 202
GART – GLOBAL SPECIES REGISTER BUTTERFLIES
Project ID: 01LC9904 (EDIS, Subproject INGE).......................................................................... 204
INGE – INVENTORY OF GEOMETRID MOTHS OF EUROPE (INSECTA, LEPIDOPTERA): PROVIDING
MULTIMEDIAL DATA ON SYSTEMATICS, ZOOGEOGRAPHY AND ECOLOGY
Project ID: 01LC9904 (EDIS, Subproject OBIF) .......................................................................... 206
OBIF - OPTIMIZATION OF BIODIVERSITY INFORMATION FACILITIES ON APPLICATION-ORIENTED
RESEARCH
Project ID: 01LC9905 (GLOPP)................................................................................................... 208
GLOPP - GLOBAL INFORMATION SYSTEM FOR THE BIODIVERSITY OF PLANT PATHOGENIC
FUNGI
Project ID: 01LC9905 (GLOPP, Subproject IT) ........................................................................... 210
GLOPP-IT - BIOINFORMATICS AND DEVELOPMENT OF THE INFORMATION SYSTEM
Project ID: 01LC9905 (GLOPP, Subproject LIT)......................................................................... 212
GLOPP-LIT - DIGITIZATION OF IMPORTANT EUROPEAN HOST-PATHOGEN INDICES FOR FUNGI
Project ID: 01LC9905 (GLOPP, Subproject Oomycetes).............................................................. 214
GLOPP-OOMYCETES
Project ID: 01LC9905 (GLOPP, Subproject Erysiphales)............................................................. 216
GLOPP-ERYSIPHALES - A GLOBAL INFORMATION SYSTEM FOR THE BIODIVERSITY OF POWDERY
MILDEWS (ERYSIPHALES, ASCOMYCOTA)
Project ID: 01LC9905 (GLOPP, Subproject Smut Fungi) ............................................................ 218
GLOPP – SMUT FUNGI
Project ID: 01LC9905 (GLOPP, Subproject Uredinales) .............................................................. 220
GLOPP-UREDINALES - REGISTRATION OF TROPICAL AND SUBTROPICAL RUST FUNGI
Project ID: 01LC9906 (INFOBOT)............................................................................................... 222
INFOBOT - DEVELOPMENT AND TESTING OF INFORMATION SYSTEMS COVERING DIFFERENT
ASPECTS OF BOTANICAL COLLECTIONS
Project ID: 01LC9906 (INFOBOT, Subproject INFOCOMP/TYPES).......................................... 224
COMPOSITAE TYPES IN GERMAN HERBARIA
THE BOHLMANN-FILES – A DATABASE OF NATURAL SUBSTANCES IN THE COMPOSITAE
Project ID: 01LC9906 (INFOBOT, Subproject BoGART) ............................................................ 228
BOGART - THE INFORMATION SYSTEM OF THE BERLIN BOTANIC GARDEN
7
Project ID: 01LC0026 (AlgaTerra) ............................................................................................... 230
ALGATERRA – AN INFORMATION SYSTEM FOR TERRESTRIAL ALGAL BIODIVERSITY: A
SYNTHESIS OF TAXONOMIC, MOLECULAR AND ECOLOGICAL INFORMATION
Project ID: 01LC0026 (AlgaTerra Subproject 1) .......................................................................... 232
THE ALGATERRA INFORMATION SYSTEM INCLUDING THE IDEAL-MODULE
Project ID: 01LC0026 (AlgaTerra Subproject 2) .......................................................................... 234
ACCESSIBILITY OF THE EHRENBERG COLLECTION
Project ID: 01LC0026 (AlgaTerra Subproject 3) .......................................................................... 236
MOLECULAR AND ECOLOGICAL DATA OF TERRESTRIAL DIATOMS
Project ID: 01LC0026 (AlgaTerra Subproject 4) .......................................................................... 238
DOCUMENTATION OF CHARACTERS OF TERRESTRIAL ALGAE IN CULTURE: R-DNA SEQUENCE
ANALYSES, MORPHOLOGY AND CRYOPRESERVATION OF REFERENCE STRAINS
Project ID: 01LC0026 (AlgaTerra Subproject 5) .......................................................................... 240
CHARACTERISTIC DATA OF GREEN AND BLUEGREEN AERO-TERRESTRIAL ALGAE
(CHLOROPHYTINA AND CYANOBACTERIA)
APPENDIX ...................................................................................................... 242
Index of Names ............................................................................................................................. 242
BIOLOG-Coordinators ................................................................................................................. 246
8
9
Introduction to the Programme BIOLOG
Erwin Beck, University of Bayreuth, Dept. of Plant Physiology
Universitätsstr.30, D-95440 Bayreuth
BIOLOG: The history BIOLOG is Germany’s most outstanding scientific and also financial response
to the UN-Convention on Biological Diversity, the so-called 1992 RIO-Convention. It is part of the
Federal Government’s programme for research into aspects of global change. In 1997 the German
National Committee for Global Change recommended the launching of a research programme on
“biodiversity in the scope of global change”. In April 1999 the guidelines of the “BIOLOG”
programme and a call for projects were published. From 251 proposals 93 projects were finally selected
which started their work during the course of the last year. Affiliation with other programmes on
biodiversity research, e.g. the ICSU and IUBS Diversitas programme is expected.
Biodiversity is severely threatened by man There is general agreement among scientists and politicians
that biodiversity is severely threatened by the activities of the tremendously increasing human
population. Estimates of the worldwide loss of biodiversity range between 2 and 130 species per day.
Even if a number on the lower side of this range is assumed, the extinction rate by far exceeds that at
the end of the Cretaceous when the Saurians died out.
Research into biodiversity is therefore not a luxury but a necessity. It is a major challenge, not only
because of the great variety of related scientific aspects and the multitude of disciplines involved, but
also with respect to the high expectations of the public as to practically applicable results and solutions
of urgent environmental problems arising in the context of global change.
Only a small part of the kingdom of organisms is known at present A primary problem in
biodiversity research is the lack of basic knowledge. Estimates of the total number of species on earth
range between 5 and 100 million, of which less than 2 million are known at present. Therefore further
inventories are indispensable, but it is clear that the progress in knowledge can hardly keep up with the
rate of disappearance of species. This holds in particular for the realm of microorganisms where we are
just beginning to develop methods for identifying organisms which can not be cultivated in the
laboratory.
The value of biodiversity is inestimable, and proper use of it has not yet been accomplished If only a
minor part of the estimated wealth of organisms is known, can we validate biodiversity and especially
the losses of biodiversity? In an economic sense this is not possible and from the biological viewpoint
we must admit that the value of biodiversity is in fact inestimable. With each extinct species a
functioning genetic system is lost and with it genes and gene products which could have been of great
importance for the needs of the rapidly growing human population. This holds for all kinds of
organisms, but will be illustrated only by the example of crop plants: Of 270,000 species of higher
plants about 3000 serve as food plants, and of these 3000 only 30 species are more or less domesticated
crop plants of which only the 4 species maize, rice, wheat and potato produce more than 70 % of the
food supply for 6.4 billion people. There is no doubt that even in the food sector man is making not yet
proper use of the resource biodiversity.
Biodiversity and the stability of ecosystems Biodiversity is understood as a major factor involved in the
stability of ecosystems. However, the investigation of the functional roles of all species of an ecosystem
is not feasible because of our incomplete knowledge of the respective biological inventory. Therefore
so-called key species must be identified which may be essential for the ecosystem under consideration,
and their specific functions in that ecosystem must be elucidated. This kind of research is not only
necessary for the understanding of functionality in an ecosystem, but also for the recognition of
environmental changes which may destabilize or even cardinally change the ecosystem.
10
Biodiversity and sustainable use of an ecosystem Stability of an ecosystem is the primary prerequisite
for its sustainable use by man. Sustainable use means an almost indefinite number of possibilities of
exploitation, which depend on the ecosystem itself and the people living in it. Important examples with
special regard to global change are the worldwide endangered tropical mountain forests which gather
the rain for the benefit of the lowlands and the semiarid ecosystems which are threatened by
desertification. Even in these ecosystems knowledge of functionality of individual organisms, such as
presumptive key species is still missing.
The Pilot Phase: Research into biodiversity requires capacity building It is conceivable that research
into the already mentioned and additional topics of biodiversity, especially with respect to global
change, will require special scientific and logistic capacity and in particular sufficient time.
The scientific advisory committee which has been in charge of the arrangements of BIOLOG has
therefore given priority to R&D projects which are based on a fair degree of international integration
and capacity building. Especially with respect to these requirements and to a strong and effective
cooperation of the disciplines involved, the committee’s policy was - and still is - to concentrate the
projects to regions expressing different levels of biodiversity, regions of different levels of relevant
knowledge and expertise, and of differently endangered biodiversity.
BIOLOG Africa and BIOLOG Europe Projects operating in several regions of subsaharian Africa (the
so-called BIOTA-projects: 3 individual projects and 3 clusters of projects with a financial volume of
30.1 million DM) and, as an antipole, projects in Central Europe (“BIOLOG EUROPE”, comprising 13
projects, awarded grants of 14.2 million DM) were selected to start BIOLOG. A pilot phase of 3 years
should be used to develop scientific as well as logistic capacity, and to examine the methodologies. A
geographical information system (GIS) will be established in order to model scenarios when sufficient
data become available.
In the BIOTA projects special emphasis is given to biodiversity inventories at different scales,
combined with long-term monitoring in so-called “observatories” which are standardized plots of
usually 1 km2 left untreated or subjected to selected kinds and varying intensities of utilization. In the
BIOLOG EUROPE projects, the effects of fragmentation of the landscape, of intentional and accidental
invasion of exotic species and other impacts on biodiversity are being studied, as well as the diversity,
dynamics and function of microorganisms in terrestrial ecosystems. In both BIOLOG EUROPE and
BIOLOG AFRICA special attention is given to history and tradition, policy implications and socioeconomic dimensions of the loss and preservation of biodiversity.
BIOLOG: Biodiversity Informatics In addition to the projects investigating aspects of terrestrial
biodiversity, a subprogramme “Biodiversity Informatics” was advertised. An immense amount of
organismic data are deposited at the big Natural History Museums, e.g. at Frankfurt ,Berlin, Stuttgart,
Braunschweig and Munich and also at smaller specialized collections of biological materials. A
substantial part of these data is not yet readily accessible or is incomplete; in particular molecular
genetic data is still missing for the majority of the collected specimens. The aims of “Biodiversity
Informatics” are to improve the methods of biodiversity data capture and maintenance, to digitalize and
network the wealth of the already available data collections, and to interlink biodiversity-related
infrastructures nationally and worldwide. Out of 56 drafted proposals 31 were selected and have been
organised within 7 projects with a financial volume of 14.6 million DM. Three of these projects
represent major clusters comprising e.g. insects, pathogenic fungi and special families of higher plants.
The BIOLOG Status Symposia Status Symposia will report on the achieved scientific progress of the
projects, on their difficulties, their future plans and on their collaborative integration. The first
symposium of this series in December 2001 will be of special interest with respect to the announcement
of the main phase of BIOLOG.
11
BIOLOG EUROPE
A MULTIDISCIPLINARY APPROACH TO EUROPEAN BIODIVERSITY
Volkmar Wolters & Barbara Reinhold-Hurek
As a continent, Europe is unique in the extent to which its natural and cultural landscape is fragmented
into a patchwork pattern. Humans have deliberately changed Europe’s environment for hundreds of
years (1). Today there is no place in Europe below 2000 m that has not been affected by anthropogenic
impacts in one-way or the other. The resulting mosaic of natural, semi-natural and cultural landscape
fragments is a major factor supporting European biodiversity by creating a complex spatio-temporal
pattern of ecosystems and habitats (2). There is growing concern, however, about potentially
detrimental consequences of large-scale changes on biodiversity, because recent alterations induced by
human beings occur at unprecedented rates and scales (3, 4).
Biodiversity is the variety of life at all levels of biological organization (5). The 13 projects of
BIOLOG-Europe that are currently funded study the impact of environmental change on Europe’s
biodiversity by focusing on a diversity of ecosystems such as floodplains, grasslands, coastal areas,
forests and arable fields (Tab. 1). The following topics are covered:
•
Evolution, maintenance and alterations of biodiversity as a result of natural and
anthropogenic changes in global environmental conditions;
•
Links between biodiversity and both ecosystem stability and functioning;
•
Development of indicator systems for rapid survey and evaluation of biodiversity change;
•
Policy dimensions of biodiversity.
Ecological processes observed and quantified in experimental approaches include secondary
succession, invasion of non-native species, changes in landscape complexity, modifications in water
availability, manipulation of diversity, and management. The various taxa considered include woody
and herbaceous plants, grasses, different guilds of insects, soil biota, basid iomycetes, and terrestrial
microorganisms. In addition, past biodiversity is reconstructed from historical sources, policy
dimensions of biodiversity are studied, socio-economic aspects of preserving biodiversity are explored,
evolutionary consequences of hybridisation are evaluated, and strategies for the conservation of genetic
resources are developed.
A particular focus is on the development and adaptation of methods for analysing diversity,
dynamics and function of prokaryotic microorganisms in terrestrial ecosystems. Prokaryotes
exclusively or predominantly catalyse key transformations within the global N, S and C cycles, and
significantly contribute to the mineralization of xenobiotics. According to recent estimates more than
half of the earth’s biomass is stored in prokaryotes. Conservative estimates suggest that only 0.4 % of
the naturally occurring species of the Archaea and Bacteria have yet been described, making
prokaryotes by far the most unexplored group of organisms. This apparent lack of knowledge reflects
the limitations of traditional techniques for studying complex microbial communities. Only a minority
of the naturally occurring prokaryotes can be obtained by classical enrichment and cultivation methods.
Recently developed DNA- or RNA-based techniques allow studying the diversity, in situ population
structure and activity of complex microbial consortia. Special attention will be given to those terrestrial
prokaryotes, which are essential to global nitrogen and sulphur cycling and/or to the production of
greenhouse gases.
12
Table 1: Overview over the 13 BIOLOG-Europe projects that are currently funded.
Monitoring and Complexity Analysis
•Biodiversity and spatial complexity in agricultural landscapes under global change.
•Biodiversity change in salt meadows.
•Ecology and biodiversity of fungi in Central Europe (with emphasis on Homobasidiomycetidae).
Experimental Approaches and Process Analysis
•Biodiversity and ecosystem function in grasslands – the effects of insect herbivory and draught
stress.
•Biodiversity and Ecosystem Function: Grassland as a Model System.
•Biodiversity of terrestrial microbiota.
•Evolutionary consequences of biological invasions – Hybridisation between invasive and native
Brassicaceae taxa.
•Global change-dependent spread of neophytes: relationship to ecosystems change (case
study: floodplains of the river Rhine).
Temporal Dimensions and Socio-economic Perspectives
•Mechanisms of biodiversity changes of selected functional groups in Red oak ecosystems.
•Management concepts based on biodiversity change during a 20-years succession.
•Reconstruction of historic biodiversity from archival sources.
•Conditions of a biodiversity-oriented policy for Germany as a part of the EU.
•Use and conservation of genetic resources: A strategy for preserving biological diversity?
References
(1) Wolters V (1999) Terrestrische Ökosysteme. Spektrum der Wissenschaft 2/1999 (A), 35-39.
(2) Troumbis A, Beier C, Wolters V, Peter D (2001) Terrestrial ecosystem research in Europe: Lessons from the European
Union’s research policy 1996-2001. Environmental Science & Policy 4, 51 – 58.
(3) Brown JH, Morgan Ernest SK, Parody JM, Haskell JP (2001) Regulation of diversity: maintenance of species richness in
changing environments. Oecologia 126, 321-332.
(4) Wolters V et al. (2000) Effects of global changes on above- and belowground biodiversity in terrestrial ecosystems:
Implications for ecosystem functioning. BioSience 50, 1089-1098.
(5) Sala OE et al. (2000) Global biodiversity scenarios for the year 2100. Science 287, 1770-1774.
13
BIOLOG AFRICA
BIODIVERSITY MONITORING TRANSECT ANALYSIS IN AFRICA
BIOTA AFRICA (Biodiversity Monitoring Transect Analysis in Africa) is the joint frame for the
three BIOLOG projects, BIOTA West Africa, BIOTA East Africa and BIOTA Southern Africa, and
altogether includes 34 sub-projects.
BIOTA AFRICA is the synonym for the corporate identity of these BIOLOG projects and subprojects, aiming at coherent research concepts and monitoring activities in various regions of
Africa. During the planning phase, a number of basic paradigms were defined which finally
resulted in a large number of research proposals:
•
The programme puts a major focus on the assessment, understanding and prediction of the
change of biodiversity on the African continent, in the context of global environmental change
and impacts of human land use. In the main phase, approaches towards a scientifically based
management and monitoring of biodiversity will gain higher importance. The programme will
not be restricted to one in-depth case study, but will aim at comparable results derived from
various scales and different regions.
•
In order to record the driving factors controlling biodiversity, research sites were selected along
the predominant environmental gradients, allowing better comparability by standardised scales
and measurement techniques.
•
The programme will strengthen the impulse towards the initiation of a global network of
standardised biodiversity observation sites.
•
Biodiversity in various land use systems is being analysed, monitored and compared with
natural systems.
•
Continental scale and ecosystem scale will be linked by suitable research structures that allow
an up- and down-scaling.
•
The major biomes of the continent will be represented.
•
Special emphasis is placed on research close to the boundaries or transition zones between
major biomes, as these regions might show more rapid changes of biodiversity.
•
The possibility of the extrapolation of results will be optimised by putting a clear focus on zonal
ecosystems of a larger spatial representation.
•
The strong interdisciplinary structure of the project aims at the development of integrative,
applied management and monitoring concepts.
Based on these goals and paradigms, as well as on the already existing disciplinary and regional
competence and co-operation, a regional concentration along two large continental transects in
Southern and Western Africa was combined with regional investigations into altitudinal gradients,
aridity as well as land use intensities in Eastern Africa and Southern Arabia.
14
The research areas are shown in the figure below, and cover the following vegetation zones in Southern
and Western Africa:
• Mediterranean Fynbos (South Africa)
• Arid mediterranean Succulent Karoo (South Africa)
• Arid tropical Nama Karoo (South Africa and Namibia)
• Arid and semi-arid Savanna types (South Africa, Namibia, Burkina Faso)
• Dry Forest (Namibia, Burkina Faso, Benin, Ivory Coast)
• Forest (Burkina Faso, Benin, Ivory Coast)
• Rain Forest (Ivory Coast)
In Eastern Africa and Southern Arabia, the following larger ecological units are covered by the subprojects of BIOTA Eastern Africa:
• Humid Highland Forest (Kenya, Uganda)
• Montane Rain Forest (Kenya)
• Coastal Rain Forest (Kenya, Tanzania)
• Semi-humid Savanna Types (Kenya)
• Wetlands (Kenya, Tanzania, Uganda)
• Xerotropical deciduous and semideciduous woodlands (Yemen, Socotra)
The realisation of the above mentioned research goals will be linked with capacity building in all
countries involved, with the participation of stakeholders wherever possible, and with an innovative
data sharing protocol. The project has been accepted as an IBOY (International Biodiversity
Observation Year) core project, and aims at providing scientific data to the Convention on
Biodiversity (CBD) and the Convention to Combat Desertification (CCD).
Fig. 1: Research areas of BIOTA AFRICA
15
BIODIVERSITY INFORMATICS IN THE BIOLOG PROGRAMME
Walter G. Berendsohn
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
w.berendsohn@bgbm.org; http://www.bgbm.org/BioDivInf/
Key words: biodiversity informatics, BIOLOG programme
Abstract:
This article briefly introduces the field of Biodiversity Informatics in general and details the role of this
discipline within the BIOLOG programme.
Introduction
“Biodiversity Informatics” circumscribes the application of IT tools and technology to biodiversity
information, principally at the organismic level. It thus deals with information capture, storage,
provision, retrieval, and analysis, focusing on individual organisms, populations, and taxa, and their
interaction. It covers the information generated by the fields of systematics (including molecular
systematics), evolutionary biology, population biology, behavioural sciences, and synecological fields
such as pollination biology, parasitism, and phytosociology. Biodiversity Informatics is considered a
part of biological informatics sandwiched between - and strongly overlapping with - environmental
informatics and molecular bioinformatics. [1]
BIOLOG and Biodiversity Informatics
The choice to include a section titled Biodiversity Informatics in the BIOLOG Programme [2] was
undoubtedly influenced by the German government’s decision to become a founding member of the
Global Biodiversity Information Facility (GBIF). The rationale for the GBIF [3] also applies to
BIOLOG: vast information resources have to be accessed, and are produced, by a biodiversity research
programme of BIOLOG’s scale. An effective electronic information infrastructure is the prerequisite
for an efficient dissemination of results and a sustained effect of the invested research funds. Effective
access to past research results and to data resources like collections is essential because it will provide
the necessary background for knowledge gain from the new results. The information provided has to be
of high quality, it must be accessible, relevant, accurate, complete, referenced, and affordable. [4]
BIOLOG’s sub-programme on biodiversity informatics is to explore and develop the very substance
of information processing present in the German biodiversity research infrastructure. It is a showcase
for the biodiversity research community’s ability to co-operate, both within the community and with
related fields such as geography and climatology. With its explicit funding focus on biodiversity
informatics, BIOLOG treads new ground. The US, Australia, Mexico and the UK, among others,
have started funding such research much earlier than the German government, but on a project-byproject basis. However, the results are so convincing that, for example, the Australian government is
now providing Au$ 10 million to digitise all Australian herbarium specimens over the next 5 years in
the Australian Virtual Herbarium [5].
The novelty of the topic in Germany is perhaps also the reason for the explicit limitation of the subprogramme to 3 years, while the rest of BIOLOG is seen as a potential a long-term programme. The
stated intention is to integrate biodiversity informatics research into the general terrestrial biodiversity
research programme. In some cases this has already happened: there are several projects within BIOTA
and BIOLOG-Europe with a distinct focus on biodiversity informatics. Further build-up of the
infrastructure and the provision of base data is to be covered by other programmes such as GBIF16
Germany, and eventually the biodiversity informatics infrastructure is to be institutionalised to ensure
long term sustainability.
For the planned creation of a system of German GBIF nodes [6], we can already draw on the results of
several projects in the sub-programme. The following EU-funded projects are also directly supported
by collection-oriented activities in the BIOLOG programme: ENHSIN (The European Natural History
Specimen Information Network [7]), BioCASE (A Biological Collection Access Service for Europe
[8]), and the forthcoming ENBI (European Network of Biodiversity Information) [9].
Presentation of projects
The sub-programme on biodiversity informatics consists of 7 main projects, 4 of which are more or less
closely-knit assemblages of sub-projects; in total 24 individual projects are funded. Most of the
investigators participating in the ongoing projects were brought together in 3 workshops to discuss
options for collaboration and for the presentation of the sub-programme in the Statusseminar. For the 3
time slots available within the conference’s programme, workshop participants generally preferred a
coordinated thematic focus to a sequence of individual project presentations. The three subject areas
selected cover (i) the discovery and identification of organisms with various computer-aided methods,
(ii) the documentation and data mining of the enormous information resources represented by biological
collections in Germany, and (iii) a summary of efforts directed at the interoperability and networking of
the project results as well as their inclusion and interrelation with international projects such as the
aforementioned European initiatives and GBIF. This selection of activities is to be supported by a range
of horizontal subjects, such as software development, georeferencing, IPR, and data quality aspects.
After little more than a decade of development, the field of Biodiversity Informatics is still in its
infancy. Presently the overriding objective – also within BIOLOG - is to provide a sound and
effective information management infrastructure for biodiversity and Global Change research. The
BIOLOG sub-programme is already making an impact in that respect. Although many
methodological, operational, and research questions remain to be solved, BIOLOG represents an
important step towards the realization of a vision: mobilizing existing biodiversity information
resources and the application of new methods of analysis to that information will stimulate new
insight into the evolution, functioning and role of ecosystems and their components.
References:
1. Berendsohn, W.G. 2001: Biodiversity informatics. Pp. 89-94 in: Contributions to Global Change
Research: A Report by the German National Committee on Global Change Research. Bonn.
2. BMBF 2000: Federal Ministry of Education and Research. Announcement of the Funding Regulations
Governing "Biodiversity and Global Change (BIOLOG)" under the Federal Government's Programme on
"Research for the Environment" of 7 April 1999.. – Bonn.
3. GBIF 2000: Business Plan for the Global Biodiversity Information Facility. Discussion Draft 5. –
Canberra. [ http://www.gbif.org ]
4. Berendsohn, W. G. 2001 ["1998"]: Problems and perspectives in biological databasing. In: Feral, J. P.
(ed.): Concepts and methods for studying marine biodiversity. Oceanis 24: 319-334.
5. Fagg, E. 2001 [Oct. 26]: Australia’s Virtual Herbarium. Canberra. [http://www.anbg.gov.au/avh.html]
6. Berendsohn W. G. (ed.) 2001 [Oct. 12]: GBIF-D. Berlin. [www.gbif.de]
7. NHM 2001: ENHSIN. London. [http://www.nhm.ac.uk/science/rco/enhsin/]
8. BioCASE 2001: BioCASE work programme on-line. [www.biocase.org]
9. ENBI 2001: European Network for Biodiversity Information, Information Site. Amsterdam.
[http://www.faunaeur.org/enbi/info.html]
17
18
BIOLOG EUROPE
19
Project ID: 01LC0002
1.3.2001 - 31.12.2003
GLOBAL CHANGE-DEPENDENT SPREAD OF NEOPHYTES: RELATIONSHIP TO
ECOSYSTEM CHANGE (CASE STUDY: FLOODPLAINS OF THE RIVER RHINE)
Rainer Lösch, Ulf Schmitz, Guido Dericks
Abt. Geobotanik, H. Heine-Universität Düsseldorf, Universitätsstr. 1, D-40 225 Düsseldorf
loesch@uni-duesseldorf.de; http://www.geobotanik.uni-duesseldorf.de
Key words : neophytes, floodplains, Lower Rhine, C4 plants, increase of vegetation period,
Amaranthus, Solanum, Impatiens, plant productivity, competition, river flood regimes
Abstract:
Biodiversity changes due to increased neophyte colonization of river banks and floodplains in the Lower
Rhine area are described and their impact upon ecosystem peculiarities assessed.Generally, these habitats
show high short-term changes of a-diversity from disturbances by the river activity and diaspore inputs via
the waterway. Under the global change effects of extended vegetation periods due to increased average
temperatures and of changed flood regimes, the spread of neophytes in these habitats is intensified. The
impact of these processes on the floodplain vegetation can be indicative for future trends and for perspectives of stability of this ecosystem complex. .
Since few decennia the percentage of C4 centrospermic taxa and of other neophytes of subtropical origin on
the river banks has considerably increased. In other floodplain vegetation units high growing neophytes are
spreading. This is mapped, and the competition dynamics of autochthonous taxa and of already established
resp. of recently spreading neophytes is analyzed both by detailed biomass harvests in the floodplain vegetation units and by pairwise cultivation of relevant species pairs under controlled conditions (obliquewindow rhizotrons, moisture gradient beds). Nutrient relations both of the soils and the plants are quantified, the impact of submergence, waterlogging or drought upon the neophytic species is measured by ecophysiological methods, and the environmental requirements for growth of these taxa are determined (germination studies, productivity measurements, biometric measures). First results after half a year duration
of the project are presented as examples for the approaches of this study.
Results:
Mapping of the Rhine river bank vegetation of the Lower Rhine area with emphasis on neophyte
colonization is underway. A locality-point map of Amaranthaceae and Chenopodiaceae on the
Rhine river banks between Dormagen and
Duisburg is given in Fig. 1. If relevées from the
second half of the 20th century are com-pared
(Tab. 1), the increase of neophytes of these
families is obvious. Only Amaranthus
retroflexus from temperate North America is
naturalized in the area since the 19th century.
The other taxa, with meridional/subtropical
origins started spreading only two decennia
ago. Another nine Amaranthus spp. of tropi-cal
origin have not yet left the bridgeheads of
harbors. A similar situation is given with
various Solanum species. The floodplain forest
understory is, among other neophyte facies,
dominated by four Impatiens species. Only I.
noli-tangere is indigeneous, and in one location
(KNEBEL
1998)
it
meets
since
Fig. 1: Centrospermic taxa on Lower Rhine river banks
20
few years I. capensis, the vicariant taxon from
temperate North America: It would be a unique
case of documentation if we could follow in the
next years a hybridogenic introgression among
this trans-atlantic species pair from the very
beginning onwards.
Tab. 1: Number of neophytic Amaranthaceae and
Chenopodiaceae in Polygono-Chenopodietum
relevées alongside the Middle and Lower Rhine
river between 1964 and 2001.
Year
Source
Number of
species
1964 LOHMEYER & SUKOPP 1992 2
1983 MAY 1988
2
1986 W ISSKIRCHEN 1995
6
- 89
1989 LOHMEYER & SUKOPP 1992 6
1998- SCHMITZ, unpubl.
2001
10
A.blitum
A.retroflex
A.blitum
A.retroflex.
A. albus
A.blitoides
A.blit:2 ssp
A.buchonii
A.powellii
A. albus
A.blitoides
A.blitum
A.buchonii
A.powellii
A. retrofl.
A. albus
A.blitoides
A.blitum
A.buchonii
A.powellii
A.retroflex.
A.tubercul.
Ch. botrys
Ch. pumilio
Corisp. lept.
Fig. 2:Maximal shoot lengths and seed maturation
depending on sowing date of an already spread (A.
powellii) and a still ephemeral (A. spinosus) sub-tropical
amaranth.
The rhizotrone and moisture gradient cultivation of related indigenous and neophytic plants demonstrated a partly differentiated, partly competing exploitation of the growing substrate due to taxonspecific root systems and different preferences with respect to waterlogging. Biometric data are just
under evaluation; ample samples for mineral nutrient determinations are stored for the analysis.
Measurements of the photosynthetic gas exchange will be done in the following year, anatomical
studies of the Centrospermic taxa concerning Kranz-type leaf structures are already completed.
In the floodplain forest understory and in spring-inundated wet grasslands 30 permanent observation plots have been installed, in part with individuum-level mapping of the species. Biomass
harvest occurred from equally-sized nearby plots so that finally biomass data of the neophyte-free
herb layer can be compared with the stand biomass of neophyte facies.
Germination experiments rendered first approaches to causal interpretations of the colonization success of established or still ephemeral neophytes (Fig. 2): If, for temperature or river flood reasons, the
germination of the latter does not occur in late spring they cannot produce ripe seeds. However, such
suitable conditions do not occur each year, as the interannual variability of the flood regime of the river indicates (Fig. 3).
References:
Fig. 3 : Rhine water level 1996-2000: Hatched
areas indicate periods of water-free river bank
conditions suitable for amaranth germination.
KNEBEL, I. (1998): Impatiens capensis Meerb. in der Urdenbacher Kämpe – Erster Nachweis dieses Springkrautes in
NRW? – Flor. Rundbr. 32: 123-125.
LOHMEYER, W. & SUKOPP , H. (1992): Agriophyten in der
Vegetation Mitteleuropas. Schr.reihe Veget.kde. 25. Bonn.
M AY, P. (1988): Flutrasen- und Flussknöterichgesellschaften
am unteren Niederrhein. 99 S., Dipl.arb. Münster.
W ISSKIRCHEN, R. (1995): Verbreitung und Ökologie von
Flußufer-Pioniergesellschaften
(Chenopodion
rubri)
im
mittleren und westlichen Europa. – Diss. Bot. 236, 375 pp.,
Berlin-Stuttgart.
21
Project ID: 01LC0004
01.11.2000–31.10.2003
RECONSTRUCTING
SOURCES
HISTORICAL
BIODIVERSITY
FROM
ARCHIVAL
Dr. Peter-M. Steinsiek, Dipl.-Biol. Antje Jakupi, Prof. Dr. Bernd Herrmann
Institut für Zoologie und Anthropologie, Abteilung Historische Anthropologie und
Humanökologie, Georg-August-Universität Göttingen, Bürgerstraße 50, D-37073 Göttingen
bherrma@gwdg.de; http://www.anthro.uni-goettingen.de
Key words: biodiversity, nature conservation, environmental history, Brandenburg (Germany)
Abstract:
The research project aims at developing a tool for nature restoration to make more reliable
judgements on the diversity of ecosystems by considering historical biological information.
For that, proxy-data are made available out of archival sources and, standards are set
according to which historical species stocks can be compiled from archival sources, be
checked and be used as references for changes of biodiversity in the past. By taking a
landscape drained in the 18th century (Oderbruch, Brandenburg) as an example, we are
developing a method which guarantees a systematic approach to the relevant archival
sources and which allows their efficient analysis.
Results:
So far, the stocks of archives have not been consulted for measuring historical biodiversity. In
comparison with exclusively temporary data collections on biodiversity, they represent, however,
an irreplaceable source of information for reconstructing the conditions of nature in the past. The
project’s aim is to trace back the functional changes certain elements of biodiversity fulfilled for
human needs by looking at historical examples at different stages in the past. Apart from that, it is
the project’s aim to determine parameters for assessing shifts of biodiversity. By following both
aims the project addresses a deficiency of relevant research. At the same time, we will gain some
information on how reliable historical data are for the reconstruction of the fauna and flora of the
past.
In our research, the land reclamation measures in 18th century Prussia and their impacts on the
existence of plants and animals serve as an example. Land reclamation mainly served the goal to
raise agricultural production and to create new land for foreign colonists to settle on. The process
of land transformation lasted many years and transformed our sample region, the Oder marshlands
– situated east of Berlin and in the state of Brandenburg – which were formerly mainly used by
fisheries, into fertile grass and arable land. This resulted in considerable alterations of the
respective ecosystems. After more than two hundred years, we still do not know which elements
of fauna and flora actually disappeared.
Our investigations focus mainly on the stocks of the former Prussian State Archives in Potsdam
and Berlin-Dahlem. The significance of archival records relating to certain questions on matters of
historical species diversity varies considerably and thus, the records need to be classified to
guarantee that they can be used in an efficient way. For the adequate processing of archival
sources we are developing a catalogue of features which should allow to distinguish between
types of sources which contain a lot of information relevant to us and types of sources which are
irrelevant.
22
In general, there are no proper historical lists of species which will give us an immediate idea of
the fauna and flora of the “Oderbruch” at the time of its melioration. Therefore, it is necessary to
put the main emphasis on Indicator Species which point to the existence of other species with
similar necessaries of life. The concept of Keystone Species allows to concentrate the search
specifically on those species the existence of which determines significantly the structures and
functions of an ecosystem.
The reconstruction of fish stocks of the Oder river in the marshland area at six different times
compiled so far from printed sources serves as an example of possible results of our research
approach (Fig. 1). Compared to the oldest data given by Jobst (1571), Bekmann (1700) and von
Borgstede (1788), the fish stocks of the Oder river today consist of less species. The early sources
give an additional account of the existence of those fish species which are of no economic use
today but were nevertheless significant in the 18th century as, for instance, they were used for pig
and poultry mast. Incidentally, the differences of species numbers in the sources concern mainly
species with low frequency. We assume that their decline was principally caused by the effects of
the growing trade and industry along the Oder river since the middle of the 18th century, rather
than by the drainage measures.
Numbers of Fish Species According to Printed Sources
40
35
30
25
20
15
10
5
species found
additional species
species missing
17
Ul
8
ric
h1 8
W
83
ittm
vo
0
nd
ac
k
em
Bo
18
rne
75
18
82
N
Jo umb
bs er
Be
t
km 1571
vo
n
an
n
Bo
rg
17
ste
00
de
Co
m
pil
ed
Mi
nim
um
0
Fig. 1: The reconstruction of the Oder fish stocks in the area under investigation represents a
successful example of consulting historical data for the assessment of species diversity.
Knowledge gained by research in archives may be utilized not only in a regional context, such as
the “Oderbruch”, but over an above that, for those developing countries which came under
European influence at a rather early stage in history. As far as land reclamation happened by
colonial powers and files have been stored according to European tradition attempts to reconstruct
ecosystems might be based on the experience of our pilot study.
23
Project ID: 01LC0005
01.10.2000 – 30.09.2002
BIODIVERSITY CHANGE DURING 20 YEARS OF SUCCESSION: IMPLICATIONS
FOR MANAGEMENT OF RESTORATION
Hartmut Koehler, Josef Müller
Universität Bremen, Zentrum für Umweltforschung und Umwelttechnologie (UFT)
Leobener Str., 28359 Bremen
a13r@uni-bremen.de; http://www.uft.uni-bremen.de/oekologie/hartmutkoehler.2.htm
Key words: succession, long-term monitoring, biodiversity, vegetation, seed bank, soil fauna, Gamasina,
restoration, management
Abstract:
The succession of soil mesofauna and vegetation has been studied since 1980 on two experimental plots, one left
for undisturbed succession, the other managed as a lawn until 1987 (thereafter ruderalization). The results
document very different patterns for soil mesofauna and vegetation, both at group and species levels. Within the
first three years, recultivation leads to a transitory pioneer-maximum, followed by a steady increase over more
than 10 years. The development of vegetation depends on historical conditioning of the seed bank, whereas
survivors of fauna are of minor importance. Although above- and below-ground plant structure influence the
development of soil mesofauna, their dynamics are not synchronised. Diversity of predatory mites (Acari,
Mesostigmata: Gamasina) is increasing over long periods of time, whereas the seed bank is impoverished.
Restoration management with focus on biodiversity should consider processes in the soil, and allow or introduce
patchiness diversified in space and time.
Results:
The former rubble-and-debris dump in the vicinity of Bremen (Northern Germany) was covered in the late
1970ies with loamy sand (C= 1.6 – 3-5; N= 0.1 – 0.3). In 1980, the plateau was graded and two plots were
prepared, one with standard recultivation measures (REC), the other was left for natural succession (SUC)
(Weidemann 1985).
Vegetation
The development of the vegetation was studied on permanent plots and with germination experiments
synchronously to succession, in the greenhouse and also in the field.
70
% Urtica dioica
percent of seedbank
According to the model of initial
35
floristic composition (Egler 1954), the
seedbank is the controlling driving force
45
0
of secondary ruderal succession of
Melilotus alb/off.
40
0
5
7 11 17 21
vegetation. Our results document for the
35
first time seed bank dynamics in a true
30
Tanacetotime-series and reveal substantial
25
Artemisetum„dispersal in time“, even of species with
20
species
persistent seeds (seedbank-types after
15
Thompson et al. 1997). After 15-20
10
years, the seedbank is almost derelict
Therophytes
5
concerning the former pioneer species
0
(Sinapis arvensis, Melilotus spp.).
0
5
7
11
17
21
Consequently, succession would restart
years of succession
after a disturbance without adapted
pioneer species, tolerant to limiting
factors during initial succession and
hence
important
facilitators
of
vegetation dynamics.
On the other hand, diversity in structure and pattern in later successional stages is determined by dispersal
strategies, landscape context (Rubus-invasion) and biotic interactions. Experimental disturbance (turfing) of late
successional stages lead to accelerated development of species with high vegetatitive regrowth (e.g., Agropyron
repens, Solidago gigantea). Germation from available seedbank, especially of species of proceeding succession
(e.g., dominant Urtica dioica, see inlet fig above), was greatly suppressed.
24
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
00
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
00
Mesofauna
Gamasina: abundances (ind. in tsd./m²)
Soil mesofauna has been investigated on
group and species level (Acari: Gamasina)
18
over the depth of 12 cm. For routine
16
14
reasons, sampling design was changed in
12
1994 from campaigns every three months
10
SUC
to one in October with two-fold sample
8
REC
6
size. The effect is drastic at first sight, both
4
on species numbers and on abundances;
2
however, it must be evaluated in further
0
detail, which is possible because of a three
years period with both sampling regimes in
Gamasina: species number
parallel. The results are of great importance
for monitoring in general.
35
For all investigated mesofauna groups
30
pioneer maxima are documented which
25
have been described before for early stages
20
SUC
of zootic successions (e.g. by Meijer, 1989;
15
REC
Pionieroptimum, Dunger, 1991; Ettema &
10
Bongers, 1993). These may be explained
5
by a timely limited easy availability of
0
nutrients. In the initial phase with sparse
vegetation cover, high amplitudes of
climatic factors break-up organic material
and minerals (Vitousek & Walker, 1987).
In contrast to the decrease of underground floristic diversity, species numbers of soil dwelling predatory mites
increase over 14 years. In total, 82 spp. have been found (68 on SUC, 64 on REC) with 50 common to both sites.
Early, intermediate and late species complexes were identified. Over the first decade, the similarity between SUC
and REC does not increase. Preliminary evaluations with correspondence analysis even suggest a diversification
with time.
Implications for management
With increasing human pressure on the landscape, successional sites with their specific biodiversity become
more and more restricted. Disturbance management could combine demands from the society and protection of
biodiversity. The results, which will be evaluated in much more detail in the remaining period of the project
highlight the consideration of underground floristics and soil fauna to complement above-ground developments.
Based on results from our integrated long-term study, biotic processes as well as various temporal and spatial
scales can be included in management models, which may be extrapolated to other systems than ours.
Outlook
The expertise gained in this project will be incorporated in suggestions for restoration management. Future
research and application is intended in the development of restoration approaches for undeveloped or degraded
soil (ReviTec®; Kesel et al. 1999), such as open-cast mining, intensive agriculture, urban green, Mediterranean
landscapes and savannah. Additionally to synergistics within BIOLOG-Europe, links to BIOLOG-Africa are
aspired.
References:
Dunger, W., Zool. Jb. Syst., 1991, 118, 423-447
Egler, F.E., Vegetatio, 1954, 4, 412-417
Ettema, C.H. & T. Bongers, Biol. Fertil. Soils, 1993, 16, 79-85
Kesel, R., H. Koehler, W. Heyser, T. Gödeke, in: Koehler, H., K. Mathes, B. Breckling (eds.), Bodenökologie
interdisziplinär. Springer, Berlin, etc., 1999, 173-187
Majer, J.D., (ed.), 1989. Animals in primary succession. Cambridge Univ. Press, Cambridge, etc.
Thompson, K., Bakker, J.P., Bekker,R.M., 1997, The soil seed banks of North West Europe. CUP, Cambridge
Vitousek, P.M. & L.R. Walker, L.R., in: A.J. Gray, M.J. Crawley, P.J.Edwards (eds.), Colonisation, succession
and stability. Blackwell Sc.Publ., Oxford, etc., 1987, 207-223
Weidemann, G., Verh. Ges. Ökol., 1985, 13, 751-758
25
Project ID: 01LC0006
1.4.2001 – 31.12.2003
ECOLOGY AND BIODIVERSITY OF HIGHER FUNGI IN CENTRAL EUROPE
(GERMANY)
A. Bresinsky, L. Krieglsteiner, C. Düring
Institut für Botanik, Universität Regensburg, 93040 Regensburg
abresinsky@t-online.de (Prof. Dr. A. Bresinsky, Am Katzenbichl 22, D-93161 Sinzing)
_______________________________________________________________________________
Key words: database, PILZOEK, fungi, Basidiomycetes, Hymenomycetes, Germany, Rhön,
biosphere reservation, biodiversity, ecological parameters, chorology, mycoflora
Abstract: Studies on fungal diversity in regard to ecological and chorological parameters
are carried out in the biosphere reservation Rhön and by the aid of the database PILZOEK
in general.
Results:
The database PILZOEK has been developed in order to collect and to compute data connected
with fungi. It is possible to establish lists of fungi in association with various other data like
different nutritional behaviour, substrates, habitats, soil characters, geology, microclimate,
chorological features, grid square frequency, red list status, occurrence in different areas
defined by their geological and geomorphological background (“Naturraum”; landscape
natural unit) and presence or absence in the different federal states of Germany. Information
from the database can be drawn in whatever combination. The database PILZOEK has been
checked for its capability to store and to recall data from literature and the field as well.
Nearly 1000 different sets of data have been established so far. The reference list of the
database is available via
www.biologie.uni-regensburg.de/Botanik/Bresinsky/Pilzoek_homepage.html
According to present calculation the 4185 species within Basidiomycetes occurring in
Germany contribute to the different trophic groups as follows: parasites (19%), saprotrophes
(48%) and symbionts (33%). Among the latter most of the species are involved in
ectomycorrhizal interrelationships between various trees and fungi. In case of the
Ascomycetes - the other main group within the Mycobionta - lack of knowledge about the
presence of species in Central Europe does not allow to present even tentative calculations
about species diversity in different trophic groups. Even in our country which is thought to be
explored intensively, there is a considerable lack of knowledge regarding species diversity.
This is an obstacle since fungi are important members of the different ecosystems for instance
as decomposers of wood and litter, as parasites on trees, herbals, mosses, other fungi and
animals and last but not least as symbionts interacting with higher plants, microorganisms and
animals.
The project being here in focus aims to contribute data to a “Mycoflora Germaniae Program
(MGP)” with special emphasis on ecological and chorological data linked with
Basidiomycetes (Hymenomycetes s. l.). The genus Hygrocybe will be treated in a pilot study
in the context of MGP and of the here discussed project. The expected approach in case of
Hygrocybe and other fungi is based on the evaluation of widespread publications in various
journals (partly also in books) and on a field work project in the biosphere reservation Rhön
as well. Amazingly enough, the Rhön has been neglected by mycologists to a considerable
extent so far.
26
Regarding field work in the Rhön, carried out by L. Krieglsteiner, 69 daily excursions have
been undertaken with a total crop of 1881 observed fungal species, being collected,
determined and in case of interest dried as herbarium specimens: Basidiomycetes (1128
species; i.e. Boletales 49, Agaricales 662, Russulales 101, “Aphyllophorales” 284,
Heterobasidiomycetes 109, “Gasteromycetes” 23), Ascomycetes (336; i.e. Pezizales 79,
Leotiales and other groups 257), mitosporic and lower fungi (53), Myxomycetes (81). Quite a
number of those species are to be regarded as the first records for the Rhön, others for one of
the three federal states (Bayern, Hessen, Thüringen) covered partially by the Rhön. Each of
the fungal species has been recorded together with ecological data at the place of
fructification, i.e. substrate (including host species), vegetation (if possible identified as plant
associations) and other habitat characters, soil type, including acidity and nitrification.
Fungi could be observed in about 70 different types of vegetation, 45 of them being identified
as plant associations. Some of the latter apparently never have been examined for the
presence of fungi so far, like for example in case of Betuletum carpaticae, Caricetum
appropinquatae, Caricetum paniculatae, Caricetum rostratae, Chaerophylletum temuli,
Epilobietum angustifolii, Geranio-Trisetetum and others. The fungal components of the
different habitats will be evaluated in respect to various parameters, for example share of
endangered species in different trophic groups etc.
The observations in the Rhön will also be evaluated comparatively to the adjacent landscape
natural unit “Mainfränkische Platten”, treated in the doctoral thesis of L. Krieglsteiner.
More then 16% of the species (i.e. rare species, species with concentration in montaneous
regions, on acid soils, or sensitive to fungicides) observed in the Rhön have not been found in
Mainfränkische Platten with a total of 2600 observed fungal species. An example for fungi
being sensitive to fungicides, occurring in the Rhön, however missing in Mainfränkische
Platten, are some of the smut fungi ( 6 of a total number of 13 species) like Microbotryum
marginale and M. pustulatum, both on Polygonum bistorta as their host.
References:
Bresinsky, A., Düring, C. (2001): PILZOEK, ein Erfassungsprogramm für Daten zur Ökologie und
Chorologie von Pilzen in Mitteleuropa. Z. Mykol. 67: 157-168
Krieglsteiner, L. (1999): Pilze im Naturraum Mainfränkische Platten und ihre Einbindung in die
Vegetation. Regensb. Mykologische Schr. 9: 1-901
27
Project ID: 01LC0010
1.1.2001-31.12.2003
CONDITIONS OF A RATIONAL BIODIVERSITY POLICY FOR GERMANY, AS A
PART OF THE EUROPEAN UNION
Andreas Hoffmann, Marko Ehlebe , Sönke Hoffmann, Stefanie Richter and Joachim
Weimann
Lehrstuhl für Wirtschaftspolitik, Otto-von Guericke Universität Magdeburg. Postfach 4120, D39016 Magdeburg
joachim.weimann@ww.uni-magdeburg.de , http://www.uni-magdeburg.de/vwl3
Key words: biodiversity policy, cost-benefit analysis, conservation, opportunity costs
Abstract:
The aim of the project is to determine the requirements of a rational biodiversity policy for
Germany and its institutional framework within the European Union (EU). Methods for the
evaluation of cost and benefits associated with biodiversity conservation activities will be
analysed. Furthermore, instruments for an implementation of a biodiversity policy based on
rationality and efficiency will be developed.
Results:
The global biodiversity loss is mainly an outcome of the human use of the biosphere. Exploitation
of natural resources results in social welfare benefits. However, costs associated with biodiversity
loss are widely ignored in cost-benefit analysis evaluating human activities.
From an economic perspective, biodiversity is characterized by attributes (lacking property rights,
public good) constituting a market failure. Therefore, decentralised decisions by autonomous
agents, which are typical for markets, will not provide a solution to the biodiversity decline,
instead governmental intervention is required. The aim of our project is to determine the
requirements of such a rational biodiversity policy and its institutional framework. It is crucial to
identify the aims of such policies in order to use rationality as a criterion for evaluating alternative
policies. Therefore, the determination of biodiversity functions influencing human well-being is a
prerequisite.
In a first step, we are trying to identify biodiversity measures, which could be used as a basis for
the evaluation of cost and benefits associated with biodiversity conservation activities.
Therefore a preceding task is to explore latest theoretical approaches on measuring diversity in
general (1,2,3). After having outlined aspects about its essential theoretical characters we will be
able to examine critically if some properties might be helpful in delimiting appropriate measures
of biodiversity. In this context an important and interesting objective is to find out how far
biodiversity can be a matter of calculus and where subjective valuations become inevitable. To a
certain point biodiversity policy is always based on normative decisions. The question is to define
the limits of such a normative approach in order to establish the starting point for rational
decisions in the context of real word policies. Another topic of interest is the spatial distribution of
biodiversity (regional, national) and its potential for specialization and allocation of conservation
efforts between regions and nations within the borders of the European Union. After the
identification of relationships between social welfare and biodiversity and establishing the
28
relevant policy goals, the appropriate instruments to achieve these goals in an economically
efficient way have to be chosen. Standard methods in economic theory will be used to assess the
applicability of the chosen instruments.
Based on the characterisation of a rational national biodiversity policy, also international aspects
have to be considered. The European Union assumed already many tasks related to biodiversity
conservation. However, we have to examine if this centralisation is in accordance with our
proposed rational biodiversity policy. Another area of research will be the eastern extension of the
EU. This extension provides a variety of perspectives and opportunities for a pan-European
biodiversity strategy. The efficient use of these opportunities and the required institutional
condition are of major importance. In addition, there is still the necessity to negotiate biodiversity
conservation issues with non-EU countries. Analysing the available instruments to create selfenforcing treaties will be another point of interest.
Preliminary analyses of strategies for the identification of priority areas for the conservation of
biodiversity revealed the “complementarity approach” (4,5) as the most promising. In this process
areas are ranked according their complementarity value, that is the degree to which a single area
or subset of areas represents the total number off biodiversity attributes found in the evaluated
system, or adds unrepresented attributes to already selected areas. Comparisons between the
implementation of the Habitat Directive of the EU and the complementarity approach in
identifying the most efficient network of areas for protection and specific management are in
progress. The analysis will focus on social-economic factors and their incorporation as
opportunity costs in conservation planning methods.
References:
1.
2.
3.
4.
5.
Weitzman, M. L. 1992. On diversity. Quarterly Journal of Economics 58: 157-183.
Faith, D.P. (1994): Phylogenetic pattern and the quantification of organismal biodiversity.–
Phil. Trans. Roy. Soc. Lond. B 345: 45-58.
Nehring, K. & Puppe, C. (in press): A theory of diversity. – Econometrica.
Vane-Wright, R. I., Humphries, C. J. & Williams, P. H. (1991) What to protect? - Systematics and
the agony of choice. - Biological Conservation 55: 235-254.
Pressey, R. L., Humphries, C. J., Margules, C. R., Vane-Wright, R. I. & Williams, P. H. (1993)
Beyond opportunism: key principles for systematic reserve selection. - Trends in Ecology and
Evolution 8:124-128.
29
Project ID: 01LC0012 (Subproject 1)
01.10.2000 – 30.09.2003
EVOLUTIONARY CONSEQUENCES OF BIOLOGICAL INVASIONS –
INTROGRESSIVE HYBRIDIZATION BETWEEN INVASIVE AND NATIVE
RORIPPA SPECIES (BRASSICACEAE).
Walter Bleeker and Herbert Hurka
Department of Systematic Botany, University of Osnabrück, Barbarastr. 11, 49076 Osnabrück
bleeker@biologie.uni-osnabrueck.de
Key words: AFLP, chloroplast DNA, evolution, introgression, invasive species
Abstract:
The aim of the project is to assess the evolutionary consequences of interspecific gene flow
between invasive and native plant species using the Brassicaceae as a model system. First
results of molecular analyses (cp DNA, AFLP) provide evidence for the spread of molecular
markers of an invasive species (Rorippa austriaca) via native species (R. sylvestris) and the
evolution of a new invasive taxon (R. ×armoracioides) via hybridization. Such ongoing
processes may even be reinforced in the future when global warming and further habitat
destruction favour the invasion of R. austriaca and other invasive species into northern parts
of Central Europe.
Results:
Since several decades Rorippa austriaca is extending its range from southeastern Europe into
Central Europe. Clonal growth provides the capacity to compete with indigenous Rorippa
species. Hybridization is common in Rorippa (Bleeker & Hurka 2001) and can be expected
along the invasive and native species contact zones since several hybrids between R.
austriaca, R. sylvestris, and R. amphibia have been reported. One of these hybrids, R.
×armoracioides (putative hybrid R. austriaca × R. sylvestris) is itself invasive in man-made
habitats in northern Germany and adjacent regions (Bleeker, in press). The aims of the project
are to study invasion dynamics of R. austriaca, to localize contact zones between R.
austriaca and native Rorippa species, to analyse interspecific gene flow using molecular
markers, and to assess the evolutionary consequences
Several native R. austriaca populations in the Tchech Republic, Hungary, and eastern Austria
and invasive R. austriaca populations in Germany have been visited in 2001. In southeastern
Europe R. austriaca occurs in flood-plain meadows and at ruderal sites. Invasive R. austriaca
populations in Germany were predominantly found along disturbed river banks and in urban
and industrial sites. Contact zones between the invasive R. austriaca and the native R.
sylvestris were detected along disturbed river banks of the rivers Ruhr and Main. The
morphologically intermediate R. ×armoracioides was found at several locations either with or
without its putative parent species, R. austriaca and R. sylvestris.
Maternally inherited chloroplast DNA (trnL intron) and biparentally inhereted AFLP markers
(amplified fragment length polymorphism) were used to analyse interspecific gene flow
between the invasive R. austriaca and native Rorippa species. First results provide substantial
evidence for hybridization and introgression between R. austriaca and R. sylvestris. Analyses
of noncoding chloroplast DNA (trnL intron, trnL/F spacer) showed that the trnL intron of
R.austriaca is characterized by a deletion of 169 bp in length. (Bleeker et al., accepted).
R.austriaca shows a trnL intron length variant of approximately 420 bp, the native species R.
amphibia, R. sylvestris, and R. palustris are characterized by a trnL intron length variant of
30
R. xarmoracioides
R. austriaca
R. sylvestris
Fig. 1: Distribution of two trnL intron length variants in the invasive R. austriaca, R. ×armoracioides,
and R. sylvestris from contact zones with R. austriaca (from Hurka et al, in press).
590 bp. The 420 bp length variant typical for R. austriaca was found in ten indivíduals of R.
sylvestris which were collected in contact zones between both species indicating hybridization
and introgression of the R. austriaca chloroplast into R. sylvestris (Fig. 1). The
morphologically intermediate R. ×armoracioides showed either the chloroplast character of R.
austriaca (26 individuals) or the character of the native species (16 individuals, Fig. 1).
AFLP analysis provided further evidence for introgressive hybridization between the invasive
R. austriaca and the native R. sylvestris and the spread of R. austriaca characters via the
hybrid R. ×armoracioides. Hybrid zones were localized at the river Ruhr near Mülheim and at
the river Main near Würzburg. 102 individuals were analysed so far. We were able to identify
several species specific AFLP markers of R. austriaca and R. sylvestris. In an invasive R.
austriaca population at the river Ruhr, three hybrids could be detected showing an additive
banding pattern of diagnostic R. austriaca and R. sylvestris markers (Tab. 1).
Tab. 1: Distribution of selected diagnostic R. austriaca and R. sylvestris AFLP markers in three hybrids
collected in an invasive R. austriaca population near Mülheim.
Primer combination /
Fragment length
Mse I-CTA / Eco RI-AAC
52 bp
54 bp
56 bp
99 bp
103 bp
110 bp
112 bp
117 bp
137 bp
194 bp
236 bp
269 bp
R. austriaca
invasive
hybrid
1
hybrid
2
hybrid
3
R. sylvestris
native
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
-
References
Bleeker, W.: Entstehung und Etablierung hybridogener Sippen: Drei Fallbeispiele aus Nordwestdeutschland. Schriftenreihe NEOBIOTA, in press.
Bleeker, W. and Hurka, H. (2001): Introgressive hybridization in Rorippa (Brassicaceae): gene flow
and its conequences in natural and anthropogenic habitats. Molecular Ecology 10: 2013-2022.
Bleeker, W., Weber-Sparenberg, C., and Hurka, H.: Chloroplast DNA variation and biogeography in
Rorippa (Brassicaceae). Plant Biology, accepted.
Hurka, H., Bleeker, W., and Neuffer, B.: Evolutionary processes associated with biological invasions
in the Brassicaceae. Biological Invasions, in press.
31
Project ID: 01LC0012 (Subproject 2)
01.10.2000 – 30.09.2003
EVOLUTIONARY CONSEQUENCES OF BIOLOGICAL INVASIONS –
ONGOING HYBRIDIZATION PROCESSES BETWEEN CARDAMINE TAXA AT
THE URNERBODEN, CENTRAL SWITZERLAND
Barbara Neuffer, Herbert Hurka, Ulrich Mönninghoff
Department of Systematic Botany, University of Osnabrück, Barbarastr. 11, 49076 Osnabrück
neuffer@biologie.uni-osnabrueck.de
Key words: AFLP fingerprinting, evolution, introgression, invasive species, vegetational records
Abstract:
The aim of the project is to assess the evolutionary consequences of interspecific geneflow
between invasive and native species using the Brassicaceae as a model system.
Natural hybrids between Cardamine rivularis auct. non Schur (2n = 2x = 16; genome RR) and
C. amara L. (2n = 2x = 16; genome AA) were discovered in 1971 at the Urnerboden in
Switzerland (Urbanska et al. 1997). The triploid hybrid C.x insueta Urbanska forms vigorous
populations in man-made, fertilised hay meadows which were created early in the 20th
century. This hybrid species by polyploidisation gave rise to the the hexaploid C. schulzii
Urbanska (genome constitution RRRRAA) which also occupies only man-made habitats
(drainage ditches), and its origin may not be older than 50 - 70 years when farmers began to
drain land. Molecular studies carried out in our group (cp- and nrDNA, isozymes, RAPD)
confirmed the hybridisation event and established the precise parentage of C.x insueta
(Neuffer & Jahncke 1997, Urbanska et al. 1997, Franzke & Mummenhoff 1999). We have
evidence for multiple origin, introgressive hybridisation and recurrent formation. In 1994, the
lowland species C. pratensis L. s .str. (2n = 4x = 30) was first observed at the Urnerboden
and since then began to spread. The invasion of C. pratensis into the Urnerboden has already
led to the formation of contact zones with the native species. This might have direct or
indirect evolutionary consequences. A new natural experiment in evolution has started at the
Urnerboden and offers the rare opportunity for long term monitoring of the evolutionary
impact of an invading species along altitudinal belts.
Results:
Distribution of Cardamine pratensis and contact zones between C. pratensis and the
indigenous Cardamine taxa have been carefully mapped, and permanent plots for long term
monitoring have been established. The plots enclose different species compositons with
regard to the Cardamine species (Tab. 1). In each plot, the actual vegetation has been
Tab. 1:
Field observations and sample numbers at Urnerboden, Central Switzerland, during the vegetation period
2001. Ca = Cardamine amara, Cr = C. rivularis, Cp = C. pratensis, Cs = C. schulzii, Ci = C. x insueta; n =
number of individuals; veg. survey = vegetational survey following Braun-Blanquet.
permanent plots
1a
1b
1c
1d
1e
2a
species
Ca/Cp
Ca/Cp
Ca/Cp
Ca/Cr
Ca/Cr/Cs Ci/Cr
Cr/Ci/Cs Ca/Ci
Cp
n (silica gel)
15/15
17/17
16/17
17/16
39/2/28 17/17
11/11/11 16/16
10
n (cytology)
5/5
5/5
5/5
5/5
5/5/5
5/5
5/5/5
5/5
5
n (seeds)
3/1
0/0
1/11
2/2
1/2/5
8/8
1/5/2
5/8
3
n (living plants)
2/2
2/0
0/0
0/0
2/0/2
3/0/0
0/3/3
0/0
2
veg. survey
yes
yes
yes
yes
yes
yes
yes
yes
yes
32
2b
2c
3a
analysed by the method of Braun-Blanquet.
Samples from individual Cardamine plants within -and in addition also outside - the plots
have been collected for several purposes: for cytological studies, for AFLP fingerprinting and
DNA sequencing, and seeds for fitness studies.
Fig. 1: Distribution of the invasive Cardamine pratensis and the „indigenous“
Cardamine species at Urnerboden in 2001.
AFLP technique has been optimised for Cardamine. In a first experiment, we compared the
AFLP banding patterns of living material, silica dried material, and herbarium material. It
turned out that silica dried leaves revealed high quality banding patterns comparable with the
results of fresh material. This is an important result which makes sampling a lot easier.
In another set of experiments we searched for intra- and interspecific variation in the AFLP
patterns. Knowledge of the genetic variation is a prerequisite for analysing the evolutionary
processes at the Urnerboden. Summarising the results, we now know (1) that all species
involved in the evolutionary scenario at the Urnerboden display species-specific AFLP bands,
(2) that hybridisation and introgression can be detected, (3) that geographic variation within
species is obvious allowing to analyse colonisation events. Hybrid nature of C.x insueta and
C. schulzii was verified by AFLP as with other molecular markers before, and analyses of C.
pratensis contact zones are in progress.
References
Franzke, A. & Mummenhoff, K (1999): Recent hybrid speciation in Cardamine
(Brassicaceae). Conversion of nuclear ribosomal ITS sequences in statu nascendi.
Theor.Appl. Genet. 98, 831-834.
Hurka, H., Bleeker, W., Neuffer, B. (in press) Evolutionary processes associated with
biological invasions in the Brassicaceae. Biological Invasions.
Neuffer, B. & Jahncke, P. (1997): RAPD analyses of recent hybrid speciation events in
Cardamine (Brassicaceae). Folia Geobot. Phytotax. 32, 57-67.
Urbanska, K.M., Hurka, H., Landolt, E., Neuffer, B. & Mummenhoff, K. (1997):
Hybridization and evolution in Cardamine L. (Brassicaceae) at Urnerboden (Switzerland):
biosystematic and molecular evidence. Plant Syst. Evol. 204, 233-256.
33
Project ID: 01LC0013
1.1.2000 – 31.12.2003
BIODIVERSITY AND ECOSYSTEM FUNCTION IN GRASSLANDS - THE
EFFECTS OF INSECT HERBIVORY AND DROUGHT STRESS
Prof. Dr. Wolfgang W. Weisser
Institut für Ökologie, Friedrich-Schiller-Universität Jena, Dornburger Str. 159, 07743 Jena
wolfgang.weisser@uni-jena.de; http://www.uni-jena.de/biologie/ecology/
Key words: Biodiversity, Ecosystem function, Nutrient cycling, Grassland, Experiment
Abstract:
This project is a collaborative research effort of the Friedrich-Schiller-University and the
Max-Planck-Institute for Biogeochemistry, both in Jena, and the Bureau for Ecological
Studies in Bayreuth/Erfurt. Using grasslands in Thuringia, Germany, as model ecosystems,
the project is designed to investigate the relationship between genetic/phenotypic diversity
and ecosystem processes such as carbon and nitrogen fluxes. Seven research groups in the
areas of plant physiology, biogeochemistry, entomology, mathematical modelling, plant
taxonomy, mycorrhizal biology, and landscape modelling will use empirical and theoretical
approaches to investigate the importance of biodiversity for the stability and functioning of
ecosystems. Characteristic for this research network will be a close cooperation between the
various research groups for example by using the same sites for empirical investigations.
Results:
In the past decades, it has been increasingly realised that species are disappering from our
planet at an unprecedented rate. This extinction crisis has raised the question how the loss in
biological diversity affects the functioning of ecosystems. First experimental studies in the
past years have shown that a loss in biodiversity will have negative consequences for
ecosystem function. For example, a number of studies have shown that decreasing the number
of plant species in a grassland will decrease biomass production and will adversely affect
other processes in the ecosystem. These studies have also made clear, however, that a general
understanding of the relationship between biodiversity and ecosystem processes can only be
gained if research programs include both various groups of organisms (e.g. animals, plants,
microbes) and different levels of organisation (e.g. leaf, ecosystem, landscape) (Fig. 1, left).
The present research network with its six subprojects therefore uses an interdisciplinary
approach to address current scientific questions. Grasslands in Thuringia, Germany, will serve
as model ecosystems and all research groups will focus their activities on the same set of
natural and manipulated grasslands.
In an experimental approach (simulation of a disturbance) the project aims to unravel
causal relationships between the genetic and phenotypic diversity and ecological processes in
grasslands (Fig 1, middle). The project will be the first to investigate the importance of insect
herbivory for ecosystem processes in stressed vs. unstressed ecosystems. In an exemplary
manner, various biological disciplines will work together to quantify both various aspects of
biological diversity (subprojects MYKO, ENTO, GENMOD, STOFF) and important
ecological processes (subprojects PHOTO, GENMOD, STOFF), using modern technology.
In addition, the results obtained will be implemented in a spatial model that will find
application in landscape planning procedures (subproject RAUM, Fig. 1, right).
34
Levels of organisation
Research approach
Subprojects in network
Landscape
RAUM
Indication
Ecosystem
Insects ? Plants ?
Microorganisms
Diversity
Soil
Processes
MYKO
ENTO
GENMOD
STOFF
PHOTO
GENMOD
STOFF
= Disturbance
Fig. 1: Schematic view of research approach and links between subprojects in network
In the first year (2001) of the study, ca. 80 different grasslands in Thuringia at a range of plant
species diversity will be visited in a collaborative effort of subprojects ENTO, GENMOD,
STOFF and RAUM. The biodiversity of these grasslands will be charakterised, in particular
plant species richness and abundance, vegetation structure and insect diversity. In addition, a
number of parameters that characterise ecosystem processes both above- and below ground
(C- and N-cycling, temperature, humidity), the ecophysiology of dominant plant species (e.g.
N- and C-use, leaf water potential) and the field sites (exposition, land use etc.) will be
measured. The results of the study will reveal first insights into the relationship between
biodiversity and ecosystem function. The results will be implemented in a spatial model
(RAUM). Further activities in the first year are the development of suitable methodology to
investigate the genetic diversity of selected plant species in grasslands, and the development
of a first mathematical model on the relationship between biodiversity and ecosystem function
(GENMOD).
In the second and third year (2002 and 2003), results from the first year will be used to select
grasslands at different levels of diversity in which experimental manipulations will be carried
out. In each of 20 blocks, the following manipulation will be carried out:
TT+
Ins+
Ins-
Water regime unchanged
Simulated drought
Insect herbivory unchanged
Insect herbivory reduced
5m
TIns +
TIns -
T+
Ins +
T+
Ins -
5m
In plots with where drought stress is applied, mobile roofs will be placed over the grassland
for a short while in spring. In plots with reduced insect herbivory, insecticides will be applied
repeatedly. All subprojects will collaborate in measuring ecosystem processes in all
experimental plots.
35
Project ID: 01LC0013 (Subproject STOFF)
1.1.2001 – 31.12.2003
THE CARBON AND NITROGEN CYCLES IN GRASSLAND ECOSYSTEMS OF
DIFFERENT DIVERSITIES
Ansgar Kahmen, PD Dr. Nina Buchmann
Max-Planck-Institut für Biogeochemie, P.O. Box 10 01 64, 07701, Jena
akahmen@bgc-jena.mpg.de, buchmann@bgc-jena.mpg.de; http://www.bgc-jena.mpg.de
Key Words: biodiversity, ecosystem function, pools, fluxes, C, N, nutrients
Abstract:
The subproject STOFF investigates the influence of biodiversity on bio-geochemical
ecosystem functions in well-established, managed grasslands, with particular emphasis on the
effect of plant and insect diversity on carbon and nitrogen pools and fluxes. In the first part of
the project, relevant site and ecosystem parameters have been measured at 80 sites in the
Thuringian Schiefergebirge to evaluate the relationship between plant diversity and productivity
at the regional scale. These data will also serve as criteria for the selection of experimental sites
in the second part of the project, when the influence of biodiversity on ecosystem resistance and
resiliance will be tested at the local scale. First results show that plant diversity is lowest in
grasslands with high and low productivities, whereas grasslands with highest levels of plant
diversity show only average productivity.
Results:
Research within the subproject STOFF is conducted at two different spatial scales. During
the 1st year, investigations are covering the entire region of the Thuringian Schiefergebirge
(regional scale). During the 2nd and 3rd year, app. 20 sites will be selected for an experimental
approach (local scale). During the first part of the project (2001), a well defined dataset has been
collected, to determine the relationship of biodiversity and ecosystem functioning at the regional
scale. Thus, data collection in summer 2001 at all 80 sites focused on the determination of
taxonomic and structural plant diversity (e.g., species composition, life forms, LAI, height, etc.)
and ecosystem parameters (e.g., carbon and nitrogen pools in the soil and the vegetation, soil pH,
soil moisture, nutrient availability in the soil as well as nutrient concentrations of plant tissues of
different species). In addition, this large dataset will be used to select the 20 experimental sites
for the second part of the project (2002-2003).
In the 2nd and 3rd year, causal relationships between biodiversity and ecosystem function
will be determined at our app. 20 sites, focussing on resistance and resiliance of grassland
ecosystems with respect to experimental disturbances. By manipulating the water regime and the
abundance of insects and invertebrates, we will determine if well-established, managed
grasslands with low levels of biodiversity are less resistant and resiliant to disturbances than
comparable systems with higher diversity levels.
Since several decades, ecologists have tried to reveal the relationship of biodiversity and
ecosystem productivity in order to understand fundamental determinants of biodiversity
(Mittelbach, et al., 2001). Species richness is often hypothesized to produce a hump-shaped or
uni-modal relationship with productivity (Grime, 1973). Our results from the first part of this
sub-project support this hypothesis for managed grasslands. Grasslands with low as well as very
high productivities showed low plant diversities, whereas grasslands with intermediate
productivity support the highest levels of plant diversity (Fig. 1).
36
plant species richness (#)
40
Borstgrasswiese
30
Bergwiese
Fettwiese
20
mixed association 1
mixed association 2
mixed association 3
10
mixed association 4
0
0
100
200
300
400
500
600
standing biomass (gdw/m2)
Fig. 1. The relationship of productivity (standing biomass until June) and plant diversity (species
richness) shows highest diversity at intermediate productivity . The investigated grasslands were
classified in three distinct vegetation types and a number of mixed associations.
Revealing the functional mechanisms of this relationship is the main goal of the ongoing data analysis. Our interest focuses on the following questions:
1) Why is there a relationship between diversity and productivity?
2) Why do we observe highest diversity at intermediate productivity?
We expect these analyses to give relevant information on resource use and competition among
different plant species present in managed grasslands under natural conditions. If functional
groups or individual species identity will dominate this pattern, will be one of the anticipated
outcomes.
References:
Grime, J. P. 1973. Competitive exclusion in herbaceous vegetation. Nature 242: 344-347.
Mittelbach, G. G. et al. 2001. What is the observed relationship between species richness and
productivity? Ecology 82 : 2381-2396.
37
Project ID: 01LC0013 (Subproject RAUM)
1.1.2000 – 31.12.2003
A SPATIAL MODEL FOR INDICATING ECOSYSTEM FUNCTIONS
Volker Audorff
Büro für ökologische Studien, Alexanderstr. 5, 95444 Bayreuth
volker.audorff@bth.de; http://www.bfoes.de/zebene2/leistg/bsp/biodiv.htm
Key words: Biodiversity, grasslands, indicator system, modelling, geographic information
system (GIS)
Abstract:
Based on measurements of the relationship between biotic and abiotic variables and
ecosystem function, the subproject RAUM aims at developing a predictive indicator system
during 2001 and 2002. By using few and easily measurable variables, the indicator system
will allow to predict certain ecosystem processes within grasslands with the help of a
Geographical Information Systems (GIS). This model will be validated in the field in
cooperation with the other subprojects in 2003. In addition, the subproject RAUM will
spatially visualisize the results obtained in the entire research network using (GIS) and
analyze vegetation data obtained in 2001. In the first year, 80 sites were studied in close
cooperation with the subprojects STOFF, ENTO and GENMOD. First results show that the
investigated grasslands can be differenciated into three distinct plant associations. In order to
obtain additional information on managemetn history, a questionnaire for the landowners is
currently developed.
Results:
Subproject RAUM will utilize the dataset that was collected in first project phase in
2001 for the development of an indicator system (2002), in which taxonomic and structural
parameters of plant diversity are used to draw conclusions on ecosystem function in wellestablished managed grasslands in montane regions of central Germany. This indicator system
will allow to predict certain ecosystem processes within these grasslands based on the
measurement of easily measurable variables. This model will be validated in the field with the
help of GIS by testing its spatial prediction in the second phase of the project (2003). After
further development, the model will be made available for applications in landscape
modelling and landscape planning. Thus, the subproject RAUM explicitly aims at a
knowledge-transfer from science to the applied sector.
In cooperation with the other subprojects a broad dataset was collected in the year
2001 as a basis for the described model. The data collected include taxonomic and structural
plant diversity such as the Ellenberg Indicator Values, life forms and plant functional groups,
species cover, LAI, height and species composition as well as core ecosystem parameters such
as carbon and nitrogen stocks in the soil and vegetation, soil moisture, available nutrients (N,
P, Ca, K, Mg, S) and soil acidity. Also, the plant associations of the investigated grasslands
were characterized by using species composition and abundance.
First results of the first project phase show that three distinct grassland associations
(Borstgraswiese, Bergwiese und Fettwiese) with different ecosystem properties such as
standing biomass and species diversity (see subproject STOFF) can be distinguished. Using
GIS, we spacially visualized these results in order to obtain an overview of the research area
(Fig. 1).
38
An additional goal of the first project phase is to inquire detailed information on the
management history of the 80 investigated sites. For this reason, we have developed an explicite
questionnaire for the responsible management agencies and farmers. From this questionnaire, we
will obtain information on harvest method and intensity, fertilizer applications, age of the
ecosystem and participation in special management and conservation programms. These data will
not only be essential for the indicator model, but are also of fundamental interest for the
cooperating subprojects and the selection of the 20 core sites for the further experimental
manipulations.
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Fig. 1: Location and species richness in the grassland communities visited in 2001
(2 x 2 m-Plots).
39
Project ID: 01LC0013 (Subproject ENTO)
1.1.2000 – 31.12.2003
THE RELATIONSHIP BETWEEN INSECT HERBIVORIE, PLANT DIVERSITY,
INSECT DIVERSITY AND ECOSYSTEM PROCESSES
Carla Wytrykush, Dr. Jörg Perner, Prof. Dr. Wolfgang W. Weisser
Institut für Ökologie, Friedrich-Schiller-Universität Jena, Dornburger Str. 159, 07743 Jena
wolfgang.weisser@uni-jena.de; http://www.uni-jena.de/biologie/ecology/
Key words: Herbivory, Insects, Ecosystem Function, Insecticide, Experiment, Grassland
Abstract:
Only recently has the significance of insect herbivores for the structure and dynamics of plant
communities been recognized. There is some evidence that manipulating plant communities has
an effect on insect herbivores, but little research has been performed to demonstrate the effects of
insect herbivory on ecosystem processes. The purpose of the ENTO subproject is elucidate the
role of above-ground insect herbivory for the structure and dynamics of the plant community.
We will investigate the relationships between insect herbivory, plant diversity and ecosystem
functioning and stability. Insect herbivore pressure will be removed using insecticides and the
effects on natural grassland ecosystems determined. The manipulation of insect herbivory will
also help us to determine the role of insects in ecosystem responses to stress (drought). In
addition, the dependence of insect diversity on plant diversity will be determined for grasslands
of different diversities.
Results:
It is generally accepted that large, plant-eating (herbivorous) vertebrates exert a great influence
on vegetation dynamics. The importance of herbivorous insects for the structure and dynamics of
natural plant communities has, however, only recently been recognized and investigated.
Manipulative studies where insects were removed using biocides have shown that herbivory may
not only change the diversity and composition of plant communities but also affect the direction
of succession. In addition to trophic effects that arise directly or indirectly from removal of
biomass by herbivores, herbivorous insects may also affect ecosystem processes through a
number of non-trophic effects. Surprisingly, there have been very few studies on the effects of
insect herbivores on ecosystem processes. The most convincing evidence that herbivores affect
ecosystem processes comes from direct manipulations of herbivore populations. For terrestrial
systems, such experiments have provided some clear demonstrations of significant effects of
herbivores on ecosystem processes such as primary productivity and nutrient cycling, but so far
they have only been performed with a focus on large mammalian herbivores. In almost all of the
recent biodiversity-ecosystem function field experiments, the emphasis was on the manipulation
of plant diversity only. Because insect herbivory will depend on plant species diversity, it can be
hypothesized that insect herbivory will modify the relationship between plant diversity and
ecosystem processes.
While insects affect plant communities, the plant community itself exerts a strong influence on
the diversity of the insects associated with the plant community. Virtually all ecological models
predict that an increase in resource diversity supports a more diverse array of consumers.
Similarly, increased herbivore density is predicted to result in an increased diversity of parasites
and predators but there may also be direct effects of plant diversity on parasite and predator
diversity as well as changes in the interactions between herbivores and their natural enemies.
Thus, it is likely that increasing plant diversity affects the diversity of the whole insect complex,
but the relationship can be complicated.
40
The subproject ENTO has the following goals:
1. To manipulate the presence of herbivorous insects in grassland plots to study the
relationships between plant diversity, insect herbivory and ecosystem processes. The
manipulation of insect herbivory will also help us to determine the role of insects in
ecosystem responses to stress (drought).
2. To assess insect diversity in the experimental plots to investigate the relationship between
the compositional structure of the insect complex, plant diversity and plant productivity.
In the first year (2001) of the study, ca. 80 different grasslands in Thuringia at a range of plant
species diversity were visited in a collaborative effort of subprojects ENTO, GENMOD,
STOFF and RAUM. The task of this subproject is to quantify insect diversity in these plots.
Suction samplers in connection with 'biocenometers' were used to obtain standardized samples
of the insect fauna. The specimens in the samples will be separated into taxonomic groups.
For certain taxa, all individuals will be determined to species level (Heteroptera,
Auchenorrhyncha, certain groups of Diptera and Coleoptera etc.). The data will allow us to
determine the relationship between plant diversity, plant productivity and insect diversity in
these grasslands.
In the second and third year (2002 and 2003), results from the first year will be used to select
grasslands at different levels of diversity in which experimental manipulations will be carried
out. In the herbivore removal plots (see abstract of main project), insecticides will be applied
repeatedly. Biomass production and other ecosystem processes will be measured in
manipulated and control plots to determine the importance of insect herbivores for ecosystem
function. The manipulation of insect herbivory in drought-stressed grasslands will also allow
us to determine the role of insect herbivores in the stress response of grasslands. In addition,
the insect diversity in the (unsprayed) experimental plots will be assessed to investigate the
importance of plant diversity, plant productivity and stress for the insect complex.
41
Project ID: 01LC0013 (Subproject GENMOD)
1.1.2000 – 31.12.2003
GENETIC AND PHENOTYPIC DIVERSITY AND RESPONSE TO DISTURBANCES
IN GRASSLAND SYSTEMS: MODELLING AND EMPIRICAL RESEARCH
Gottfried Jetschke, Frank Hellwig, Nidal Odat
Institut für Ökologie, Friedrich-Schiller-Universität, Dornburger Straße 159, 07743 Jena
Institut für Spezielle Botanik, Friedrich-Schiller-Universität, Philosophenweg 16, 07743 Jena
Gottfried.Jetschke@uni-jena.de; http://www.uni-jena.de/biologie/ecology
hellwigotto.biologie.uni-jena.de, b1odni@uni-jena.de
Key words: genetic diversity, phenotypic diversity, grasslands, ecosystem functions, modelling,
RAPD-PCR, reproductive success,
Abstract:
The aim of the project is a two-fold one: the development of a mathematical model to cover the
relationship between both phenotypic and genetic biodiversity and ecosystem functions and the
quantification of genetic diversity of selected species in the field. The model will include multiple
trophic levels and different functional types and shall give a conceptual answer how productivity
and nutrient cycling are related to the diversity of species. Representative species of our
grasslands will be selected to quantify genetic diversity within and between populations by
RAPD-PCR.
The modelling part covers the development of an appropriate mathematical model which shall
especially include the genetic diversity of the community and the response of the ecosystem to
external disturbances, like drought stress or removal of herbivores. The results of the model
investigations will give a more general answer how the response of the system depends on its
diversity (with respect to functional types) and on the number of trophic levels. A special
emphasis is given to the question how the degree of genetic diversity will influence the
functioning of the community under disturbances and environmental changes.
The empirical part of the project is focussed on the quantification of the genetic diversity in the
field. Typical species, like annual or perennial herbs and grasses of different growth form, will be
selected to quantify the genetic diversity by means of a RAPD-PCR. These investigations will be
performed over two years to detect possible changes after an experimental manipulation.
Moreover, the genetic diversity will also be measured by using quantitative traits of reproductive
success, as pollen and seed production and vitality and differences in germination success.
Results:
A basic model structure has been developed to include several functional types of primary
producers plus an additional trophic level of herbivores with different food preferences. The
model is explicitly based on the uptake of a resource (e.g. nitrogen), different ways of utilisation
by the plants and cycling through local and regional pools. It can be shown that the indirect
interactions through local resource use can support the coexistence of a certain number of species
and, therefore, maintain diversity. Some other results obtained until now show that herbivores
may essentially influence the performance of the community (net primary productivity) by their
feeding behaviour and spatial mobility.
42
First attempts have been made to include the genetic structure and its change between
generations. Growth throughout the year is modelled in continuous time, but reproduction
(and genetic mixing) is a discrete event based on random mating, seed production and
creation of vegetation gaps by mortality. Also a spatially explicit model structure has been
tested to serve as a starting point for an individual-based modelling approach.
About 14 species have been sampled from the potential sites to check which of them are most
suitable to be used for the genetic investigations. The genetic analysis by PCR is just being
performed and first results will be available by the end of the year. Based on these preliminary
screening and on the species list of the sites selected for our experiments, about five
characteristic species will be selected for the detailed genetic analysis in the next two years.
43
Projekt ID: 01LC0014
1.4.2001 – 31.3.2004
BIODIVERSITY AND ECOSYSTEM FUNCTIONS IN GRASSLAND
G. Aas 1 , C. Beierkuhnlein2 , G. Kossmann3 , E. Matzner3 , C. Neßhöver2 , A. Reuter4 and
C. Engels 4
1
Ökologisch-Botanischer Garten, Universität Bayreuth, 95440 Bayreuth;
Landschaftsökologie, Institut für Landschaftsplanung und Landschaftsökologie, Universität
Rostock, 18059 Rostock; 3 Bayreuther Institut für Terrestrische Ökosystemforschung,
Lehrstuhl für Bodenökologie, Universität Bayreuth, 95440 Bayreuth; 4 Abteilung
Agrarökologie, Universität Bayreuth, 95440 Bayreuth
2
christof.engels@uni-bayreuth.de; http://www.bitoek.uni-bayreuth.de/biolog
Grassland is an important type of agroecosystem covering about 60 million ha of land in middle
Europe. The number of plant species in grassland communities may strongly vary depending on
climate and soil conditions. In agricultural grasslands, plant diversity is strongly affected by
agricultural measures such as frequency and date of harvest, and fertilization. Recent studies have
shown that reduction of plant diversity may decrease the productivity of grassland (Hector et al.,
1999). However, up to now there is strong debate on the general validity of diversity effects, and
the mechanisms involved in the positive diversity effects found in some experimental studies
(Grime, 1997; Kaiser, 2000).
It is the aim of this project to assess biodiversity effects on ecosystem functions in grassland.
Biodiversity may be defined as taxonomical diversity, i.e. number of plant species, or functional
diversity, i.e. number of functional types. Ecosystem functions include net primary production,
accumulation of minerals and carbon in plants and soil, and filtering of water for ground water
formation. The importance of taxonomical and functional diversity on these ecosystem functions
is investigated in experimental grassland communities established in the field, in large lysimeters,
or in small root boxes.
In the field, biodiversity effects are measured in grassland communities that were established in
1996 on 2 x 2 m plots („BIODEPTH“ project). On 64 Plots, communities with 1, 2, 4, 8 and 16
species were established. Species were selected from 30 grassland species typical for the area. The
plots were weeded from 1996 to 1998 to sustain composition and species number. In 1999, the
weeding was stopped to allow succession to occur within the plots. The plots were under
harvested twice a year (June and September) and were not fertilized. Emphasis of the
investigation within the BIOLOG-Bayreuth project is put on the plots with communities showing
similar taxonomical and functional diversity as that in the lysimeters (see below).
In the lysimeters with a surface area of 1.3 x 1.3 m and a depth of 1 m and in the root boxes (0.2
m diameter, 1 m depth) biodiversity effects are measured in grassland communities that were
established in 2001. The plant communities are composed of either 2, 4, or 8 different plant
species that may be attributed to 4 functional groups: grasses with low growth habitus, grasses
with high growth habitus, herbs with low growth habitus and rosettes, herbs with high growth
habitus. Legumes were not included. All communities are grown in 5 replicates. In the root boxes,
in addition to the plant communities in the lysimeters, also monocultures of all species grown in
the lysimeters are cultured.
The effects of biodiversity on ecosystem functions are studied in a research group including the
disciplines botany, ecology, and soil science. The project is divided into three subprojects that
investigate biodiversity effects on (1) above-ground and (2) below-ground plant characteristics
and (3) soil characteristics and water and nutrient fluxes.
44
References:
1. Grime, J. P., Science, 1997, 277, 1260-1261.
2. Hector, A. and 33 coauthors, Science, 1999, 286, 1123-1127.
3. Kaiser, J., Science, 2000, 289, 1282-1283.
45
Projekt ID: 01LC0014 (Subproject Vegetation)
1.4.2001 – 31.3.2004
TAXONOMIC, FUNCTIONAL AND STRUCTURAL CHARACTERISTICS OF
ABOVE-GROUND PLANT FEATURES AND THEIR INFLUENCE ON
ECOSYSTEM FUNCTIONS
C. Neßhöver1* , G. Aas 2 and C. Beierkuhnlein1
1
Landscape Ecology, Institute for Landscape Planning and Landscape Ecology, University of
Rostock, 18059 Rostock
2
Ecological-Botanical Garden, University of Bayreuth, 95440 Bayreuth
* current address: Bayreuth Institute on Ecosystem Research (BITÖK), University of
Bayreuth, 95440 Bayreuth
carsten.nesshoever@bitoek.uni-bayreuth.de
Key words : biodiversity, grassland, productivity, functional diversity, structural diversity,
functional attributes
Abstract:
Biodiversity can be examined from different viewpoints. Besides the taxonomical view, structural
and functional aspects of biodiversity get more in focus, especially when regarding the
relationship between diversity and ecosystem functions.
These different aspects of diversity are the main objective of the subproject. Earlier studies in
experimental grasslands have shown, that there is a relationship between the existence of specific
functional groups of plants for example for productivity and nitrate leaching in grassland
communities. These functional relationships shall be further investigated by dividing plant species
into funtional groups and observing their performance within the communities.
Experimental questions and approaches:
How are productivity and biodiversity in grasslands related to each other? Several studies have
shown a positive correlation (e.g. Hector et al. 1999). But the debate about this relationship
remains controversial (e.g. Huston et al. 2000). For example, there is evidence that the diversity of
functional types of plants is more important than taxonomic diversity (Spehn et al. 2001). Most
studies have focused on this functional viewpoint only marginally, for functional classifications
entail several problems (Beierkuhnlein & Schulte 2000). Within the vegetation-subproject of
BIOLOG-Bayreuth, the functional classification of species and individuals and their role shall be
examined in depth, using existing data on the species and biometric measurements of individuals
in the experiments. The species composition of the lysimeter-experiment was designed according
to this aim.
Besides the taxonomic and functional aspect of diversity, also a structural aspect will be
addressed. Continuous LAI-measurements in different heights are carried out to look at the
structural development of different plant mixtures on the lysimeters and the experimental field
plots. Relating these data to biomass production, the role of a higher light use efficiency due to
more diverse temporal and spatial development of above-ground plant-organs for productivity
will be examined.
Adressing these different aspects of biodiversity it is aimed to evaluate the varying information
contents implied with premise to a prediction on ecosystem functions. As perspective, the results
of BIOLOG-Bayreuth shall be applied to the landscape scale by identifying the main traits of
grassland diversity in central Europe.
46
References:
1. Hector, A. and 33 coauthors, Science, 1999, 286, 1123-1127.
2. Huston, M.A. et al., Science, 2000, 289, 1255a.
3. Spehn, E.M., Scherer-Lorenzen, M., Schmid, B., Körner, Ch. and BIODEPTH-consortium,
Verh. Ges. Ökol., 2001, 31, 178.
4. Beierkuhnlein, C. and Schulte, A., pp. 45-64. In: Jax, K. (ed.): Theorie in der Ökologie:
Funktionbegriff und Unsicherheit in der Ökologie, 2000. Peter Lang
47
Projekt ID: 01LC0014 (Subproject Roots)
1.4.2001 – 31.3.2004
THE ROLE OF BIODIVERSITY FOR STORAGE OF CARBON AND MINERALS IN
ROOTS, AND ACQUISITION OF SOIL RESSOURCES IN GRASSLAND
A. Reuter and C. Engels
Abteilung Agrarökologie, Universität Bayreuth, 95440 Bayreuth
christof.engels@uni-bayreuth.de
Key words : nutrient uptake ability, root diameter, rooting depth, root growth, rhizodeposition
Abstract:
In this subproject the role of roots for biodiversity effects on productivity and cycling of carbon
and mineral nutrients in grassland is investigated. The effects of plant diversity on root
characteristics that are related to (a) net primary production and mineral accumulation of roots,
(b) acquisition of soil resources by living roots, and (c) release of organic carbon and nutrients
into the soil by decaying roots are assessed in grassland communities that were experimentally
established in the field, or in lysimeters, or in root boxes. It is expected that the results will
improve our knowledge about the processes involved in diversity effects on ecosystem functions,
and finally may help to develop recommendations of agricultural measures for sustainable
grassland management.
Experimental questions and methods:
Is below-ground productivity and mineral nutrient accumulation in roots affected by plant
diversity ?
Below-ground productivity is assessed by measuring root growth in a well defined period of time
using the ingrowth-core method (Oliveira et al., 2000). The soil cores are sampled twice per year
to a depth of 1 m for measurement of biomass and nutrient content of roots.
Is acquisition of soil ressources affected by plant diversity ?
To examine this question morphological root characteristics related to nutrient and water
acquisition such as root length and surface area are measured in the roots sampled from ingrowthcores (see above). Physiological root characteristics related to nutrient and water acquisition are
measured in these root samples using 15N (Buljovcic and Engels, 2001), or are indirectly assessed
from accumulation of tracers in the shoot. For the assessment of root characteristics related to
nutrient acquisition, special emphasis is put on „critical“ time periods where root growth and
activity are expected to be low (e.g. after cutting, in spring and autumn), and soil layers where
root length density is expected to be suboptimal for complete capture of soil resources (i.e. below
the main rooting zone). In the communities growing in root boxes, root growth along a transparent
interface with the soil can continuously be measured, allowing assessment of root growth with the
high resolution in time that is necessary for the critical periods.
Is carbon and nutrient release from roots to soil affected by plant diversity ?
It is to be expected that in agriculturally used grassland roots are the most important source for
carbon and mineral input into the soil. The rate of rhizodeposition is dependent on root longevity
that is affected in a species-specific manner by shoot pruning. Root longevity and root turnover
are estimated by repeated observations of the same section of the root boxes allowing to estimate
the time interval between appearance and disappearance of specific roots from the interface (Smit
et al., 2000).
48
References:
1. Buljovcic, Z., and Engels, C., Plant Soil, 2001, 229, 125-135.
2. Oliveira, M. R.G., van Noordwijk, M., Gaze, S. R., Brouwer, G., Bona, S., Mosca, G., and
Hairiah, K., pp. 175-210. In: A.L. Smit et al. (eds.) Root Research: a Handbook of Methods,
2000. Springer-Verlag.
3. Smit, A. L., George, E., and Groenwald, J, pp. 235-272. In: A.L. Smit et al. (eds.) Root
Research: a Handbook of Methods, 2000. Springer-Verlag.
49
Projekt ID: 01LC0014 (Subproject Soil)
1.4.2001 – 31.3.2004
THE IMPACT OF PLANT DIVERSITY OF GRASSLANDS ON WATER- AND
NUTRIENT-FLUXES IN SOILS AND SOIL ORGANIC MATTER
G. Kossmann and E. Matzner
Bayreuther Institut für Terrestrische
Bodenökologie, 95440 Bayreuth
guido.kossmann@bitoek.uni-bayreuth.de
Ökosystemforschung
(BITÖK),
Lehrstuhl
für
Key words: soil water- and nutrient-fluxes, leaching of NO3 and DOM, characteristics of
physical fractions of SOM
Abstract:
Main aims of this subproject are to investigate changes in soil parameters and to measure
water- and nutrient-fluxes in BIODEPTH-plots and lysimeters. The soil inventories cover
CEC, Nmin , PCAL and physical soil fractionation with subsequent analysis of the fractions. Soil
solution and leachates are collected to evaluate changes in the solution phase and to
calculate seepage fluxes of mineral elements and dissolved organic carbon.
Approach:
Does plant diversity affect water- and nutrient-fluxes in soils and soil organic matter
characteristics?
It is postulated that increased diversity causes a higher use of soil-born resources which leads
to detectable lower concentrations of nutrients in soil solution. Higher use of nutrients results
in a decrease of NO3 -leaching to the groundwater (also HECTOR et al, 1999). Furthermore,
the higher diversity is supposed to result in an increasing carbon-sequestration of the soil.
Soil parameters such as CEC, Nmin , PCAL, etc. will be investigated in an initial (autumn 2001)
and a final soil inventory in autumn 2003 on BIODEPTH-plots and lysimeters. Special
emphasis is given to physical fractionation of SOM (CHRISTENSEN, 2001) and subsequent
analysis of the fractions.
The experimental grassland communities (BIODEPTH-plots) will be used to investigate the
impacts of plant diversity on soil moisture, potential leaching of NO3 and dissolved organic
matter (DOM). Soil matrix potentials are continuously measured by tensiometers (30 and 50
cm soil depth) and soil solution is collected periodically by ceramic suction cups at 40 cm soil
depth.
The main aim of the lysimeter studies is to balance water- and nutrient-fluxes in response to
plant diversity. This task is achieved by acquisition of soil solution from 15 cm and 30 cm
depth (using suction-cups) and leachates from 100 cm depth.
Solutions are analysed on a monthly basis for mineral elements (K, Mg, Ca, Mn, Fe, Al, Na,
NO3 , NH4 , Cl, SO4 , HPO4 , HCO3 ) and DOM. The study started in May 2001, the installations
were recently finished, thus, results are not available yet.
References:
1. Christensen, B.T., 2001, European Journal of Soil Science, 52, 345-353.
2. Hector, A. and 33 co-authors, Science, 1999, 286, 1123-1127.
50
51
Project ID: 01LC0015 (Subproject VEGETATION ECOLOGY)
1.3.2001 – 29.02.2004
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE
'VORPOMMERSCHE
BODDENLANDSCHAFT'
–
SPATIO-TEMPORAL
DYNAMICS AND ECOSYSTEM FUNCTION OF BIODIVERSITY IN BRACKISH
GRASSLANDS
Dipl. Geogr. E. Held, Dipl. Biol. S. Seiberling, Dipl. Biol. R. Zimmermann, and Prof. Dr.
U. Schickhoff
Stiftungsprofessur Biodiversität, Department of Botany, University of Greifswald, Grimmer Str.
88, 17489 Greifswald
schickho@uni-greifswald.de; http://www.uni-greifswald.de/~botanik/botinst.htm
Key words: salt meadows, biodiversity dynamics, ecosystem function, multi-temporal analysis,
ecological modelling
Abstract:
The project is part of the interdisciplinary research program “Development of biodiversity of
salt meadows in the Vorpommersche Boddenlandschaft“, which is carried out by several
research groups of the University of Greifswald, the University of Bremen and the Technical
University of Berlin. Biodiversity of salt meadows on the German coast of the Baltic Sea is
investigated with regard to temporal and spatial dynamics as well as ecosystem function in
order to explore quantitative and qualitative dimensions of biodiversity dynamics.
By means of vegetation and phytomass analyses and soil samplings on different study sites
abiotic and biotic site factors and biodiversity will be correlated. Local and regional
distribution patterns of phytodiversity are assessed and human impact is evaluated. Multitemporal analyses are applied to detect previous biodiversity dynamics. GIS-integrated
modelling is developed to enable predictions of future changes of biodiversity in the salt
meadows. Studies of ecosystem function with regard to stability and productivity are based on
experiments imitating changes of environmental conditions. Experiments include simulations
of sea level change and nutrient decrease. 30 permanent plots comprising the whole variety of
salt meadows were established to assess future biodiversity changes.
Results:
1. Spatial Aspects
In the beginning of this project vegetation ecologists and animal ecologists jointly selected
nineteen study sites in order to analyse relationships between phytodiversity, zoodiversity and
environmental conditions.
In summer 2001, vegetation was analysed at each of the 360 pitfall traps, which were set by the
animal ecologists involved. Shannon-index and the Evenness-index were calculated for the
vegetation data to characterize α-diversity. Concurrently with the vegetation records, phytomass
was harvested within an area of 25 x 25 cm nearby the traps. The dry weight of the phytomass
will be used as a measure of productivity of the different study sites. Furthermore, total C, N, P,
and degree of salinity will be analysed in order to assess nutrient conditions.
Soil samples were taken at the end of the vegetation period. Examined soil samples will include
soil moisture, mineral nitrogen, available phosphorus, C/N-ratio, organic matter, and soil-pH.
According to first results there is a negative correlation between productivity and salinity. Further
evaluations are carried out to find relations between site conditions and phytodiversity.
52
2. Temporal aspects
Temporal changes in species and ecosystem diversity were investigated with multi-temporal
analyses using a geographic information system. Research data were acquired from maps of the
17th , 19th and 20th century. The evaluation of a floristic database, which is available at the
Department of Botany, shows a distinct decline for the most of the typical salt meadow species
after the intensification of land use since 1964. Further data source materials (e.g. aerial and
satellite images) were collected for the ongoing analyses.
The design of a model, which enables the development of different kinds of scenarios regarding
future biodiversity dynamics (e.g. assuming a sea-level rise), is under development stage.
To ensure and improve data exchange between the different sub-projects of the research program,
a database was designed and implementation has started. Furthermore this database serves as the
basis for the projected database “Biodiversity Vorpommersche Boddenlandschaft”, which is
going to put results of the research program at public’s disposal.
To assess diversity changes over a longer period, 30 permanent plots were established. Study sites
comprise a salinity range from 2 0 /00 to 10 0 /00 and include natural types as well as embanked sites.
Vegetation cover and above-ground phytomass were recorded and soil samples were taken to
assess the abiotic / biotic site conditions as a reference for a regular monitoring program.
3. Functional Aspects
In 2001, two of three experiments on the ecosystem function of phytodiversity with regard to
stability and productivity have been launched. Both of them focus on the functional role diversity
under changing environmental conditions (i.e. sea level change and nutrient decrease).
For the experiments, three different types of species composition growing under comparable site
conditions were selected in a 1 ha field-exclosure of a salt meadow 10 km north of Greifswald.
Type 1: poor (4 grasses and herbs), type 2: rich (7 grasses and herbs), and type 3: rich with
legumes (7 grasses, herbs, and legumes) serve for a comparison of different richness in species as
well as in plant functional types.
To simulate rising sea level conditions 20 sods of each type were transplanted 15 cm beneath
from their original level, 10 remained as a control and 10 were dug out and re-implanted to assess
the mechanical impact of transplantation. Previously, species cover and above-ground phytomass
were recorded for each sod.
Moreover, phytomass was measured for 20 sods and 10 controls of each type to detect the
influence of nutrient decrease due to reduced N and P import by inundation. A N/P/K-solution
will be added in next year´s early summer as a period of three years is to short to achieve a
sufficient reduction in nitrogen as the limiting factor of salt meadows by mowing. Thus, the
opposite trend will be simulated in order to draw respective conclusions for a nutrient reduction.
By the end of the vegetation period in 2001 the following site parameters are to be recorded for
both experiments: soil moisture and bulk density, mineral nitrogen, available phosphorus, soil pH,
cation exchange capacity (CEC), base saturation, C/N-ratio, and organic matter. Phytomass will
be analysed with regard to the content of water, total N, P, C, and Cl.
The data base of 2001 will serve as a reference for the experimental effects to be recorded over
the following two years.
53
PROJECT ID: 01LC0015 (Subproject LANDSCAPE ECONOMY)
1.3.2001 – 29.2.2004
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE
'VORPOMMERSCHE BODDENLANDSCHAFT' – ECONOMICAL VALUATION OF
BIODIVERSITY
Prof. Dr. U. Hampicke , Dr. R. Döring, A. Kowatsch
Fachgebiet Landschaftsökonomie, Botanisches Institut, Universität Greifswald, Grimmer Str. 88,
17487 Grefiswald
doering@uni-greifswald.de; http://www.uni-greifswald.de
Key words: Contingent Valuation, Use of salt meadowland, Costs and Benefits of Dyke building
Abstract:
The decline in biodiversity is also a great problem from an economic viewpoint. Therefore
economists search for possibilities to preserve biological diversity with economic arguments.
Within in the project there will be a calculation of a cost-benefit-analysis for a dyke-removing
project at the German Baltic Sea coast. This includes a contingent valuation for the existence
value of rare species and the costs of different use systems of salt meadowland were developed.
Results:
The decline in biodiversity is also a great problem from an economic viewpoint. It is clear that in
the future we will depend even more on stable ecosystems with a high biodiversity if todays
processes of biodiversity loss continue. Therefore economists search for possibilities to preserve
biological diversity also with economic arguments. Economic valuation of costs and benefits of
dyke building as an example must introduce not only benefits from use values of an ecosystem
changed by a different water regime, also existence values and option values are important. In
addition the costs of dyke building and the repair work must be introduced. This is the reason why
for most cost-benefit analysis only estimations of the total economic value are possible. Over the
last three decades economists developed a lot of different methods of economic valuation of
individual preferences for rare species, ecosystems or historic landscapes (for an overview about
economic valuation Gronemann und Hampicke 1997).
Within the project as part of the research on salt meadowland we calculate costs and benefits of
dyke-removing and combine the results in a cost-benefit-analysis. To compare different situations
three study areas were chosen. The Sundische Wiese, as an area where the dyke-removing is
planned, the Karrendorfer Wiesen where the removing has been realized a few years ago and the
Kooser Wiesen as a typical salt meadowland. The main use system on the meadowland is
extensive pasturing with cattle and we estimate that after a dyke-removing 50% of the Sundische
Wiese is not usable that way anymore. Also the provender quality will change after the ecosystem
will come under the water regime of the Baltic Sea again (Kowatsch et al. 1997). What this means
for the user was part of the work in the first year. In addition an overview about the literature with
respect to the history of the use systems should give us informations about the possible use
alternatives in the future and what might happen after the dyke-removing. All these parts were
introduced in a scenario for the Sundische Wiese and will be part of the cost-benefit-analysis at
the end.
During the next year we’ll prepare the questionnaires for two examinations: On one side we want
to know what people around the area think about the dyke-removing (acceptance of this) and on
the other side we want to ask tourists about their preferences for rare species after the natural
habitats will be restored. Not many contingend valuations deal with existence values and we hope
54
that we can get some useful results here. Most of the species on natural salt meadowlands are not
very common and not well known by the people (like orchids). In addition costs of dykeremoving, dyke-building and repair will be analysed over the next two years. After the first
project phase (bejond 2004) the economic part will be oriented close to the research program of
the other working groups within the project.
References
Gronemann, S. und U. Hampicke (1997): Die Monetarisierung der Natur – Möglichkeiten, Grenzen
und Methoden in: Jahrbuch Ökonomie und Gesellschaft Nr. 14 – Nachhaltigkeit in der
ökonomischen Theorie, Frankfurt (Main) (Campus), S. 164 – 207
Kowatsch, A., T. Fock, M. Köhler & J. Walter (1997): Landnutzung, Naturschutz und Küstenschutz
im Biosphärenreservat Südost-Rügen, Neubrandenburg (Schriftenreihe der FH
Neubrandenburg Reihe A), 137 S.
55
Project ID: 01LC0015 (Subproject ANIMAL ECOLOGY)
1.3.2001 – 29.2.2004
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE
'VORPOMMERSCHE BODDENLANDSCHAFT' - DYNAMICS IN DIVERSITY OF
ANIMAL SPECIES AND COMMUNITIES IN A CHANGING ENVIRONMENT
WITH SPECIAL REGARD TO DISPERSAL PROCESSES
Prof. Dr. D. Mossakowski, Prof. Dr. G. Müller-Motzfeld, A. Broll, W. Dormann, T.
Görlich, A. Hochkirch, N. Kamer, G. Mathiak, R. Mißkampf, R. Vierk, A. Ziebarth,
M. Zimmermann
Institut für Ökologie und Evolutionsbiologie, Abteilung Evolutionsbiologie, FB 2, Universität
Bremen, PF 33 04 40, 28334 Bremen
dmossa@uni-bremen.de; wdormann@uni-bremen.de
http://www.biologie.uni-bremen.de/biologie/ifoe/evolution
Lehrstuhl für Spezielle Zoologie, Ernst-Moritz-Arndt Universität, Bachstraße 11/12, 17489
Greifswald
kaefer@uni-greifswald.de; gerdmathiak@web.de; http://uni-greifswald.de/~zoologie
Key words: salt meadows, salt marshes, Vorpommersche Boddenlandschaft, Baltic Sea, pitfall
trap, lifestock taking, Carabidae, Saldidae, distribution patterns, area value rating, dispersal,
population genetics
Abstract:
Ecological analysis is used to show the significance of the distinctiveness and dynamics of
environmental factors which essentially determinate the diversity of animal species, communities
and landscape sectors. The focus will be on the impact of human activities, with special regard to
climatic changes and the dispersal abilities of animal species.
In close co-operation with the other project partners, recent developments and their influencing
factors will be analyzed by molecular techniques, field ecological (and, if necessary, laboratory)
methods, as well as a Geographical Information System (GIS).
Based on these results, prognoses (e.g. scenarios) to be used for the recommendation of measures
for the maintainance and development of biodiversity with respect to economical relations can be
statet. The following questions will be investigated:
Ø Which regional dispersion patterns characterize the diversity of species, coenoses and
landscape sectors? Are there areas with a high concentration of species that show a high
spatial significance?
Ø Are there small-scaled and regional differences in the genetical equipment of populations
that might give a hint for the genetical pauperisation due to isolation, or that reflect the
range and intensity of dispersal processes?
Ø Which are the abiotic/biotic factors to determine diversity (distinctiveness, dynamics)?
How do extreme events, such as inundations due to heavy storms, influence diversity?
How important are area size, age and isolation?
Ø Which are the historical and recent impacts of land-use type and intensity (especially
agriculture, coastal protection and tourism)?
Ø How does the dispersal ability of animals influence the distinction of biodiversity? Do the
hydrodynamics of the Baltic Sea cause a ‘hydrochoric network’ of populations or
coenoses? How far do human activities influence such a network (increasing isolation)?
Ø Which impacts can be expected of the predicted climatic changes? (direct: temperature,
precipitation, wind; indirect impacts: sea level rise, rising frequency of inundations due
to heavy storms, change of landuse type and intensity).
56
Ø Are there areas for the emigration of arthropods, which live exclusively in salt- and
coastal habitats? Can biodiversity be regenerated/re-established by a retraction of the
dike position towards inland (expansion of foreshore areas)?
Ø How can the results be used effectively within an Integrated Coastal Zone Management
(databases, GIS, expert or indicator systems, monitoring concepts)?
In cooperation with the project partners, along the “Vorpommersche Boddenküste” (investigation
region) 20 investigation areas were selected with special regard to geographical location,
geological development, hemerobiotic degree, soil type and recent agricultural use.
In the first year of study (2001), epigeic arthropods (especially Carabidae and Saldidae) were
caught at 65 investigation sites (1 to 9 investigation sites per area) with pitfall traps from the end
of March until the beginning of November. The pitfall traps were changed 16 times in 14-daysintervalls and remained outside for at most 224 days. The time consuming sorting of the animals
is still in progress. Additionally in 2001, vegetation, biomass, inundation frequency (based on a
nivellement; measuring in 14-days-intervalls) as well as several soil parameters are investigated
in cooperation with the working group of Prof. Schickhoff.
Parallel, lifestock taking of Carabids in the entire investigation region was started: Nearly 25%
of the 800 km coastline have been under survey so far. Much effort had to be done to get fresh
material for DNA extraction because of the complex spatial situation. In order to make use of
material already fixed, we tested specimens collected in ethylene glycol, the medium used in
pitfall traps in order to get species which are rare or not easily to collect.
In Carabid beetles living north of the Alps, species are known without variability in their
distribution area, others display variability from population to population. Therefore, we
screened core and mitochondrial genes of those species which were collected first from most
sites: Carabus clatratus, a species flightless and living on peat ground like coastal salt marshes
and meadows with a high content of organic matter.
Results:
First results show partly considerable quantitative and qualitative differences in the Carabid
communities between the investigation sites of the saltmarshes. Intensity and frequency of
inundation (often regulated by coastal defense management), water salinity and landuse intensity
(e.g. vegetation structure and soil compaction) seem to be the key factors for the development of
diversity in Carabid coenoses.
In general, isolated populations of Carabus clatratus show sequence differences; the highest rate
of substitutions was found in ND1 and ND5 (0.2 – 0.5 %), the lowest in the control region, ITS1
and ITS2 (about 0.1 %). Additional data exist for 12S rRNA, CO1, CO2, CO3. The most
interesting result is a relatively high genetical difference in two populations (islands Grosser
Werder and Hiddensee (Gellen)) that are about 7 km apart.
Although at this stage we are unable to interpret the pattern of variability, this flightless species
display a high degree of within species diversity.
References:
VAGTS, I., CORDES, H., WEIDEMANN, G., & MOSSAKOWSKI, D. (2000): Auswirkungen von
Klimaänderungen auf die biologischen Systeme der Küsten (Salzwiesen & Dünen), Abschlußbericht
des BMBF-Verbundvorhabens (Teil A u. B )
MÜLLER-MOTZFELD , G.; SCHMIDT , J, & BERG, C. (1997): Zur Raumbedeutsamkeit der Vorkommen
gefährdeter Tier- und Pflanzenarten in Mecklenburg-Vorpommern. – Natur und Naturschutz in
Mecklenburg-Vorpommern 33, 42-70.
57
Project ID: 01LC0015 (Subproject SOIL SCIENCE)
1.3.2001-29.2.2004
DEVELOPMENT OF BIOLOGICAL DIVERSITY IN SALT MEADOWS OF THE
'VORPOMMERSCHE BODDENLANDSCHAFT' - WATER AND SALT DYNAMICS
OF SALTY MEADOW AND MARSH SOILS
L. Ikels, K. Schwärzel and G. WessolekWessolek
Ecology Institute, Soil Science Dept., TU Berlin, Salzufer 11-12, D-10587 Berlin
Gerd.Wessolek@tu-berlin.de; Leif.Ikels@gmx.de;
http://www.tu-berlin.de/fb7/ioeb/bodenkunde
Key words: biodiversity, global climate change, salt marshes, salt meadows, physical and
chemical soil parameters, modelling
Abstract:
Climate scenarios predict a global temperature increase within the next decades. Aim of the
project is to gain insight in the future development of the salt meadows of the Vorpommersche
Boddenlandschaft, a region of outstanding ecological relevance. Analyses of new collected
data on chemical, physical and hydrological soil parameters in this region will supply the
basis for a prediction of the soil lifetime dynamics associated with climate, groundwater and
land use changes in this region.
The specific study plan of the project includes:
Exemplary soil ecological mapping of typical salt meadow areas.
Determination of relevant soil parameters for typical soil profiles.
Characterisation of the water and salt dynamics for two selected sites by means of soil
hydrological measurements and numerical model calculations.
Derivation of parameters concerning to soil water and soil salt balance as determined
by temperature, humidity, rainfall patterns, sea level height and land use due to
predicted climatic shifts.
Project description and first results:
Targets of the investigations in the first project phase have been the selection of the
appropriate study sites and of the suitable measuring probes.
For the purpose of a prolonged surveillance during the project, a transect with 10
experimental plots was set up at the “Kooser Wiesen”. Measurements of soil water contents
and soil water quality are performed every 14 days in two soil depths. The soil pore water is
subsequently analysed for pH-values, electric conductivity, concentrations of anionic and
cationic salts and nutrients. The set-up of an intensive measurement field on the “Kooser
Wiesen” is planned for next year. It is designed for measurements with higher analysis
resolution in time and space (automatic probes measurements over soil profiles).
The selection of the appropriate water content measuring devices is up to now challenging,
since most of the commercially available TDR-probes (time domain reflectometry) do not
deliver reliable values at pore water salt contents above 20 mS/cm.
A first attempt to gauge probes coated with epoxy resin seemed promising up to water
contents around 40% v/v, but since the peaty soils typical for the investigational sites may
reach values of 70% v/v, probably the use of the coated probes will not always be possible.
Next to the set-up of the measuring plots for the continuous data collection, soils of two
transects on the “Kooser” and “Karrendorfer” salt meadows have been investigated for
58
important chemical and physical soil parameters. The results of the analyses will supply,
along with water tension curves, the bulk densities, water contents, C/N ratio, organic matter
contents, salt concentrations, and pH-values of the investigated soils.
Future investigations will focus additionally on soil mapping of typical vegetation units of the
salt meadows and a subsequently soil ecological state assessment.
Modelling of the dynamic development of the soil water parameters under changing site
conditions (salt contents, climate constraints, groundwater supply and land use pressure) shall
help to answer the following questions:
1) Which consequences will arise from the current climate change scenarios in respect to
the site parameters of the ecologically relevant salt meadows?
2) How will the so-called ecological soil moisture parameter develop and how will the
length of the current dry and moist periods change in future, taking the different site
characteristics into account?
3) How will the salt meadows develop, considering particularly the scenarios predicting
recurrent situations of climate extremes (e.g., frequencies of flooding, dry periods)?
59
Project ID: 01LC0018 (SUBICON Subproject 1)
1.3.2001 – 29.02.2004
SUBICON - SUCCESSIONAL CHANGE AND BIODIVERSITY CONSERVATION IN
A RED OAK CHRONOSEQUENCE –
SUCCESSION OF DIVERSITY AND FUNCTION OF ECTOMYCORRHIZAL
TYPES IN RED OAK FOREST ECOSYSTEMS
J. Wöllecke, S. Gebhardt, B. Münzenberger and R.F. Hüttl
Chair of Soil Protection and Recultivation, Brandenburg University of Technology Cottbus,
P.O. Box 101344, D-03013 Cottbus
Jens.Woellecke@TU-Cottbus.de; http://www.tu-cottbus.de/roteichen/teilprojekte/index.htm
Key words: Dispersion of ectomycorrhizal types, Primary succession, Competition, Neophytes,
Quercus rubra, Mining, Reclamation
Abstract:
The region of Lower Lusatia (Eastern Germany) is characterized by many active and reclaimed
lignite mining sites. Of these, 15 % have been reafforested with red oak (Quercus rubra L.). Thus,
this introduced tree species is nowadays widespread on the former mining sites. As a pioneer
species, red oak is well adapted to extrem soil conditions. Like other oak species, red oak forms
ectomycorrhizae with higher fungi (Ascomycetes and Basidiomycetes). In forest stands these
mycorrhizae hold a key position for the water and nutrient cycle. The mycobionts are able to
mobilize, accumulate and transport water as well as nutrients from different soil substrates and
translocate them to the phytobiont.
The mycorrhizae and the extramatrical mycelium are part of the detritus food chain. The litter is
intensively colonised by saprophytic and mycorrhizal fungi, bacteria and soil animals like
earthworms, enchytraeid worms, nematodes, collemboles, mites and others which deminute and
decompose organic components of the soil. Thus, the nutrients are being fixed in organic
components which can be taken up very fast by the growing mycelium. The huge diversity of
fungal species is based on the fungi`s different functions in mycorrhizal symbiosis. In consequence
of the high variety of functional groups the determination of fungal species will be the first part of
the investigation. After characterization of the autecological importance of single species, their
interactions with other heterotrophic organisms will be estimated.
Questions:
The project will highlight some topics related to function, temporal and spatial dispersion of
ectomycorrhizal types. The investigations will answer aspects of the following questions:
- How does primary succession of ectomycorrhizal types work?
- Which changes in successional stages can be observed along the chronosequence?
- Are there differences in the ectomycorrhizal species composition associated to native and
neophytic oak species?
- Are there differences in the ectomycorrhizal species composition of mining substrate and
undisturbed soils?
- Which ectomycorrhizal fungi are key species for the development of forest ecosystems on
mining substrate, and how do these fungi disperse on different scales?
- How important is the role of ectomycorrhizal fungi within the detritus food chain?
Methods:
Our plots are located in former lignite mining sites reclaimed for forestry and planted with red
60
oak. The chosen Quercus rubra stands are forming a chronosequence. To show the differences in
mycorrhization between the meliorized mining substrate and naturally grown soils we prepared
also a control plot in the neighbourhood of the mining area. To interprete the role of the tree
species in community stucture of mycorrhizae, we compare our results from the Quercus rubra
stands with stands of native Quercus and Pinus species formerly investigated in the same region.
For an analysis of the ectomycorrhizal community we identify the mycorrhizal fungi on three
ways. First, we conduct a morphological and anatomical characterization of the mycorrhizae. This
makes possible a quantitative analysis of the community composition. In a second step, we use
molecular genetic techniques like PCR, RFLP and sequencing of ITS regions.
In using a capillar electrophoresis we try to analyse the content of different nutrient elements in
the mycorrhizae. These results, together with the morphological and anatomical features and the
autecological data, will give us hints to the function of a specific fungal species in this ecosystem.
First results:
After a preliminary sampling of our investigation sites we could differentiate 14 ectomycorrhizal
types by means of anatomical and morphological characterization. Till now, we were able to
determine four of these types to species level. Cenococcum geophilum is a well known fungus
establishing mycorrhizae with a very broad range of phytobiots. In comparison to other
mycorrhizal fungi, this species shows a wider ecological valency. Piloderma croceum and
Scleroderma citrinum are also known as euryoecious species. Former investigations in reclaimed
mining sites in the same region detected mycorrhizae of these three species as dominant in Scots
pine stands. A fourth species was determined as Paxillus involutus which is the dominant species
in our samples. All of these four fungi are typical early stage fungi. Thus, they were expected to
occur in this sites of primary succession.
Other mycorrhizal fungi which we identified by their fruitbodies were Amanita muscaria, A.
citrina, Boletus edulis, B. luridus and several species out of the Russulales.
In comparing natural forst stands with reclaimed mining sites our first samples indicate that root
morphology can differ between these different habitats. Nevertheless, these findings have to be
confirmed by further samples.
The first place we presented our project was the congress of the German Society of Mycology and
Lichenology (GML 20. – 23. 09.01) in Halle, Germany.
Outlook:
First we want to create a sampling design which allows an up/down scaling of the dispersion of
ectomycorrhizal types to different scales.
In experiments we want to investigate the influence of mycophagous insects on the establishment
of mycorrhizas and the development of mycorrhizal communities under grazing pressure.
Information on ongoing studies is available at www.tu-cottbus.de/roteichen/teilprojekte/tp1/index.htm.
61
Project ID: 01LC0018 (SUBICON Subproject 2)
1.3.2001 – 29.02.2004
BIODIVERSITY OF PLANT COMMUNITIES AND SELECTED PHYTOPHAGES IN
RED OAK DOMINATED ECOSYSTEMS
P. Denkinger and U. Bröring
Dr. Udo Bröring, Lehrstuhl Allgemeine Ökologie, BTU Cottbus, Postfach 101344, D-03013
Cottbus
broering@tu-cottbus.de, www.subicon.de
Key words: Biodiversity, Brown coal mining areas, Disturbance, Community Function, Heteroptera,
Succession, Quercus rubra
Abstract:
The temporal and spatial variation of biodiversity of plant communities and selected phytophagous arthropod
communities in red oak dominated ecosystems is described. Species diversity of producers (higher plants and
mosses) and first order consumers (phytophagous heteropteran species) are analysed in relation to influences of
habitat diversity, disturbance regime, biotic interactions, and genetic diversity. Aim of the project is to give
explanations for the generation, establishment, maintenance, and development of biodiversity, which should be
confirmed by experimental analyses.
Questions:
In the restoration management of post mining landscapes widespread afforestation with non native red oak
(Quercus rubra) took place. The main questions to be answered in the framework of the SUBICON research
project are:
What factors determinate the species diversity of red oak dominated ecosystems and adjacent open landscapes?
Which of the various explications of biodiversity (e.g. number of species, Shannon-Wiener-Index, Preston´s
gamma in species-abundance relations) are useful to describe biodiversity adequately in order to generate models
to explain the maintenance and development of biodiversity?
What about the relations among the biodiversity of different compartments of the communities (producers, first
and second order consumers, different sets of species with special ecological functions)?
What about the temporal variation of biodiversity at different spatial scales and which of the general theories on
the development of biodiversity in ecosystems can be confirmed as to explanatory and predictive power?
Methods:
The investigations are carried out in four former brown coal mining areas of Lower Lusatia (Eastern Germany)
as well as in an undisturbed reference.
The following investigations have already been realized:
- Analyses of vegetation at 112 SUBICON plots in red oak forests as well as in the adjacent open landscape,
- Starting of the experimental approach to recolonization after disturbance.
- Quantitative sweepnet sampling of Heteroptera (30 sites in total, 50 sweeps per 100 qm on each of the
sampling sites).
- Heteropteran species sampled by pitfall trapping will be included to analyse biodiversity of different vertical
strata.
The influence of soil disturbance on the diversity development of the vegetation in Lusatian post mining areas is
analysed by an experimental approach (see figure 1). It is widely accepted that disturbance has a strong influence
on biotic communities and their functioning on various spatial scales. In the post-mining landscape of Lusatia an
obvious disturbance factor is the wild boar (Sus scrofa) that causes soil disturbances in all vegetation structures
by churning up the soil. This kind of disturbance is imitated. The experimental approach is to analyse the
mechanisms of colonization of plants and the development of diverse communities.
Three open-cast mines of different ages and three vegetation types (red oak forests, Calamagrostis epigejos
stands, psammophytic grassland) are included into the studies, six disturbance and six reference plots per stand
are analysed (sum of study plots: N = 108). The following procedure is applied:
62
-
Soil disturbance: Removal of the above-ground vegetation. Digging up of the soil up to a depth of about
12 centimetres;
Recording of the colonizing individuals;
Soil seed bank analysis;
Exposition of seed traps.
S
R
S
R
R
S
R
S
S
R
S
R
(S = disturbance plot, 4 m², R = reference plot, 4 m², Distance between neighboured plots: 2 m)
Fig. 1: Experimental design
The results of the vegetation surveys and the second order consumers studies (TP 4) will be linked in order to
generate a general model comprising different functional groups of species.
First Results:
Previous research on open landscape vegetation show that the change of vegetation type is slow. Direct
ordination revealed that environmental parameters such as pH, the level of available phosphate and organic
carbon as well as the age of the study sites do not significantly account for the variance (Wiegleb & Felinks
2001). For succession in red oak forests no likewise studies are known.
The investigations started in summer 2001. Preliminary results can be presented at this state.
The mean number of plant species in red oak forests of the former coal mining area is 13 (4 - 38 species), in
undisturbed red oak forests 17 species (14 – 19 species), respectively, while in Calamagrostis stands 11 species
(4 – 17) have been found. In psammophytic grasslands a mean of 9 species (2 – 17) occured.
The number of heteropteran species found on oak trees is low, a very high amount of common species regularly
occurring on native oak trees in Brandenburg are missing. This is in contradiction to the sampling results of open
landscapes, where species number is high: including results of former studies roughly calculated 30 % of all
openland species occuring in Brandenburg established populations at the post mining landscape.
Outlook:
The next steps of the investigations will be:
- Continuing with the data collection,
- Determination of Heteroptera including material collected by pitfall trapping (Tp 4),
- Statistical analyses, modelling,
- Application of the experimental approach,
- Data link between sampling results for different groups of species.
References:
1.
Wiegleb, G. and Felinks, B., Applied Vegetation Science, 2001, 4, 5-18.
63
Project ID: 01LC0018 (SUBICON Subproject 3)
1.3.2001 – 29.02.2004
GENETIC DIVERSITY OF SELECTED PLANTS, PHYTOPHAGES
ZOOPHAGES IN SUCCESSIONAL RED OAK ECOSYSTEMS
AND
W. Durka, K. Heyde and M. Güth
Dr. Walter Durka, Sektion Biozönoseforschung, Theodor-Lieser-Str. 15, D-06110 Halle,
Walter.Durka@halle.ufz.de, www.subicon.de
Key words: Colonization, Genetic diversity, Molecular markers, Population genetics,
Quantitative genetics, Succession
Abstract:
The project will analyse genetic diversity of different functional groups (plants, phytophagous
bugs, spiders and zoophagous beetles) within a Red Oak chronosequence. Molecular markers will
be used to elucidate genetic diversity and population structure as a basis for the understanding of
colonization and microevolutionary processes. Genetic diversity within a population and between
various populations as well as between different species are analysed in detail and compared to
each other.
In plants quantitative genetics of morphological traits will be analysed in order to understand
adaptive evolutionary changes due to varying selection regimes (light, competition, nutrients)
during the chronosequence.
The results will be used together with those of other subprojects in a comparative analysis of the
development of species diversity and genetic diversity through time in a highly disturbed
ecosystem. The current state of the project including species selection, site selection, sampling
and laboratory progress is reported.
Questions:
The project addresses the following questions:
- How do different functional groups of plants and animals differ with respect to spatial and
temporal population structure?
- Does colonization take place through continuous immigration or through a single founder
population?
- Does genetic diversity change through the course of succession?
- Do molecular markers and quantitative traits differ in their spatial pattern and temporal
development?
- Is there microevolutionary change during succession in adaptive traits connected with
competition or light regime?
Methods:
The main method of analysis is the Amplified Fragment Length Polymorphisms (AFLP). The
AFLP technique is based on the selective PCR amplification of resriction fragments from a
total digest of genomic DNA (Vos et al. 1995).
64
Fig. 1: AFLP (Amplified Fragment Length Polymorphisms ) gel image of Rumex acetosella
sampled in the Schlabendorf-Süd area.
First results:
Species selection - plants
From a number of potential plant species two species with contrasting ecology and habitat
where selected. Whereas a number of species were present either at the beginning or at the
end of the chronosequence, only these two were present both early and late in the
chronosequence.
(1) Rumex acetosella (Sheep´s Sorrel) a herbaceous pioneer species with low competitive
ability, present in 13 sites.
(2) Calamagrostis epigejos (Wood Small-reed) a generalistic stress-tolerant grass, present in
18 sites.
Species selection – animals
Arthropods of different functional groups were selected, namely the phytophagous true bug
Stenodema laevigata, the spider Alopecosa fabrilis and the zoophagous ground beetle
Calathus ambiguus.
The sampling both for the molecular and the quantitative genetics analyses was undertaken
from July to September 2001. For molecular analyses of plants a total of 444 samples from 16
(R. acetosella) and 21 (C. epigejos) sites has been taken. For quantitative genetics 1398 plants
from 16 (R. acetosella) and 21 (C. epigejos) sites are being cultivated and cloned vegetatively
for experiments to be performed in 2002 and 2003.
Of the target animal species 97 spiders, 173 true bugs and 366 ground beetles in 5 sites were
collected for molecular analyses. Next captures will be carried out in spring 2002.
For molecular analyses, AFLP (Amplified Fragment Length Polymorphisms) are currently
being developed. Preliminary results are shown in figure 1.
Outlook:
The molecular analyses and laboratory work will be going on in 2002. Focuses on data analysis
and modelling of the data will start in autumn 2002.
References:
1.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., Vandelee, T., Hornes, M., Frijters, A., Pot, J.,
Peleman, J., Kuiper, M. and Zabeau, M., Nucleic Acids Research, 1995, 23 (21), 4407-4414.
65
Project ID: 01LC0018 (SUBICON Subproject 4)
1.3.2001 – 29.02.2004
STRUCTURE OF THE
CHRONOSEQUENCE
PREDATOR
COMMUNITY
IN
A
RED
OAK
A. Balkenhol, K. Voigtländer and W. Xylander
Staatliches Museum für Naturkunde Görlitz, Postfach 300154, 02806 Görlitz
balkenhol@debitel.net, http://www.subicon.de
Key words: Araneae, Carabidae, Chilopoda, Coal mining areas, Habitat structure, Red Oak
chronosequence, Staphilinidae, Succession
Abstract:
Araneae, Carabidae, Staphilinidae and Chilopoda are studied based on a comparative analysis of
different aged red oak stands and grassland of post mining areas. The hypothesis of habitat
structure being the most determinating factor of biodiversity of a predator community is tested.
Questions:
In the end of the 19th century, the red oak (Quercus rubra) was imported from North America to
Europe as a fast growing tree being able to achieve a high productivity on poor stands. Until now
there is only little knowledge about the importance of these forests for indigenous arthropod
predators. Several investigations have shown the importance of habitat characteristics such as
habitat structure for arthropod colonisation.
In this context, we investigate both the development of red oak forest habitat diversity and the
development of predator diversity in a chronosequence of former coal mining areas. Recultivated
areas are most suitable for investigations of biodiversity development because any established
arthropod populations affecting new colonisators are absent.
- What is the influence of tree species on the predator community?
- What are the differences between forests with indigenous species (Quercus robur, Quercus
petraea) and unforested areas on reclaimed soil?
- How much time does it take for establishment of biodiversity in disturbed landscapes?
Methods:
The colonisation of the study areas by predator groups of different mobility and dispersal strategy
(Araneae, Staphylinidae, Carabidae, and Chilopoda) is studied. The epigaeic activity of these
groups is investigated by pitfall traps and tree trunk eclectors throughout the vegetation period.
Additionally, soil cores and sweep-net catches are analysed for arthropod abundance in spring and
autumn. Expected results refer to species richness, dominance structure, habitat preference of
species, life form diversity, age structure of populations, and also species and individual mobility.
To investigate the influence of habitat structure and vegetation architecture of the forest stands on
predators, a method according to Curtis Bignal and Meißner, which analyses habitat structure and
diversity of different vertical quadrats, is applied.
The investigations are performed in cooperation with the working groups of the Brandenburgische
Technische Universität Cottbus and the Umweltforschungszentrum Leipzig-Halle. The synoptic
consideration of the diversity of mycorrhiza (in both their symbiotic and degrading aspect), of
primery producers, of selected phytophages, and of zoophages enables a comprehensive picture of
the functional relations between spatial structures and biocenotic diversity.
66
First Results:
In previous studies (Mrzljak & Wiegleb 2000) of open country vegetation of post mining areas it
was shown, that all epigaeic arthropod groups being present in the undisturbed surroundings can
be found after 20 years in considerable number and species rich community. No specific trend in
species number development was detected. Species number of a site is obviously limited by
stratification and height of the colonized vegetation type. Differentation among e.g. Calamagrostis
and other tall grass stand is not strongly developed. In Europe no comparable data for Red Oak
forests exists.
Overview:
Grassland
S U CC E S S I ON
Quercus rubra forest
Indigenous
Quercus species
Large scale
habitat structure
Small scale
habitat structure
• Ecological diversity
Araneae
• Habitat preferences
Staphylinidae
• Form of life
• Age structure
Carabidae
• Mobility of species and individuals
Chilopoda
• Influence of vegetation type
Fig. 1: Investigated arthropod groups and environmental parameters
Outlook:
Outdoor sampling and determination of material was started in July this year and will be
continued until end of 2003. First statistical analysis were carried out. Modelling of the data will
start after the end of collection.
References:
1. Mrzljak, J. and Wiegleb, G., Landscape and Urban Planning, 2000, 51, 131-146.
67
Project ID: 01LC0020 (BIOPLEX Subproject 1)
1.10.2000 – 30.9.2003
BIODIVERSITY
AND
SPATIAL
COMPLEXITY
IN
AGRICULTURAL
LANDSCAPES UNDER GLOBAL CHANGE (BIOPLEX) - MODELLING AND
GEOSTATISTICS
Kerstin Wiegand, Thorsten Behrens , Ute Visser, and Wolfgang Köhler
Biometry and Population Genetics, IFZ, U of Giessen, Heinrich-Buff-Ring 26, 35392 Giessen
Kerstin.Wiegand@agrar.uni-giessen.de; http://www.uni-giessen.de/biometrie/
Key words: landscape ecology, Geographical Information System (GIS), study site selection,
spatially-explicit simulation models, ecological profiles
Abstract:
Among several fields of activity we highlight two examples. First, we introduce PaDS, a tool for
objective study site selection for landscape ecology. PaDS showed that the landscape investigated
in BIOPLEX can be characterized by mean slope and mean perimeter-to-area ratio of the land
parcels. BIOPLEX study sites were selected spanning the entire dimension of landscape
complexity. Second, a spatially explicit, multi-species metapopulation model has been developed
which will be used to investigate the functional influence of landscape structure on biodiversity.
Results:
BIOPLEX investigates the connections among biodiversity and spatial complexity in agricultural
landscapes. This subproject combines the theoretical aspects of BIOPLEX. The fields of activity
include (1) the development and application of a standardized procedure for study site selection,
(2) the identification of meaningful landscape indices describing spatial landscape complexity as
it influences biodiversity, (3) linking geostatistical information of the other subprojects in a GIS,
(4) the development of spatially explicit simulation models facilitating a scaling up of findings
obtained at small spatial scales to the landscape scale, and (5) the development of hypotheses on
the functional influence of landscape complexity on biodiversity using spatially explicit
simulation models. Here, we present (1) and (5) in more detail.
Study site selection
In large complex landscapes the spatial representativity of sample sites becomes important as
financial and human resources of research projects limit the practible sample size. Therefore,
we developed PaDS as a tool for standardised landscape analyses to guarantee reproducible
and representative study site selection. The main objective of PaDS is to detect patches that fit
user specified criteria based on patch metrics. We explain PaDS using the selection of study
sites situated in wheat fields for BIOPLEX subprojects 2 – 4 as an example.
First, PaDS was used to examine 714 Patches of 7 different sizes ranging from (250m) 2 up
to (3000m)2 . For each of these patches a number of landscape metrics and terrain attributes
were derived from the DEM (Digital Elevation Model) and the official German land use
database called ATKIS; both converted to grids with 20m mesh size. The parameters used in
this project were: proportion of different types of land use, acreage/pasture ratio, (total)
number of parcels, perimeter-to-area ratio (PAR), Shannon Index, (derived from ATKIS), as
well as mean slope, mean global radiation, mean topographical roughness, mean altitude,
mean height above drainage channel, proportion of stretched hill slopes (derived from the
DEM) and mean precipitation. Using principal component analysis (PCA) this plethora of
68
indices and attributes was synthesized into two compound axes. The two indices describing
these axes best are the topographical parameter mean slope and mean perimeter-to-area ratio,
a parameter describing land parcel size and shape. Patch size was selected on the basis of
parameter distribution. With edge lengths of 2000m or more, land use is usually distributed
normally and diversities trend for a maximum. Additionally, PaDS was used to determine, the
relevant, structure-controlling landscape parameters for a square patch of 2000m edge length
about each of the 6960 arable land parcels. Based on this information 23 study sites were
selected which build gradients spanning the entire ranges of slope and perimeter-to-area ratio
and therefore represent the entire landscape.
Species
Richness
Functional influence of landscape complexity on biodiversity
Agricultural landscapes can be viewed as spatial mosaics of patches of arable land, meadows, and
fallow land. We investigate two opposing processes influencing biodiversity in terms of species
richness: (1) Species richness increases with patch size because local species-extinction
probabilities decrease. (2) Species richness decreases with increasing isolation among patches of
the same land use type because recolonisation is more difficult2 . As a consequence, we predict
fairly high species richness at small patch sizes (isolation negligible). As patch size increases,
isolation increases as well and local extinctions will be less likely to be counteracted by
colonisations, resulting in low species richness. However, as patch size increases further, local
extinctions will be rare and species richness very high. Finally, at very large patch sizes, isolation
becomes practically perfect and biodiversity is low even though extinctions are unlikely (Fig. 1).
The spatial scales at which the hypothesized pattern can be observed are a function of the
‘ecological profile’ of the species investigated. Ecological profiles differ qualitatively in their
dispersal ability and habitat specificity. For example, isolation decreases with increasing dispersal
ability and decreasing habitat specificity.
To investigate these hypotheses, we developed a grid-based, spatially explicit metapopulation
model. For model development, we had insects in mind. Each grid cell constitutes an area of a
specified land use type and may accommodate a number of populations of different ‘types’
(specific quantitative representation of an ecological profile). All land parcels comprise several
cells. Local populations may go extinct and recolonization is possible due to dispersing
individuals managing to establish new local populations. We will run simulations for different
landscapes using types from within one and all
ecological profiles to quantify the specific and
overall effect of landscape structure on
biodiversity. Simulation results will be
compared to the field surveys conducted by
subprojects 2 –4. Deviations between field and
model results will lead to model modifications
and thereby to increasing understanding of the
effects of landscape complexity on biodiversity.
These investigations will be accompanied by a
Patch size
second, more detailed model restricted to aphid
dynamics.
Fig. 1: Species richness as a function of average patch size.
References:
1.
2.
Behrens, T., Purtauf, T., Wolters, V., Köhler, W., and Dauber, J. 2001. Study site selection and
gradient detection in complex landscapes using an automated patch detection tool (PaDS). Pages 217220 in A. Belward et al., editors. Geo-spatial knowledge processing for natural resource management,
University of Insubria, Varese (Italy).
Kruess, A., and Tscharntke, T. 2000. Species richness and parasitism in a fragmented landscape:
experiments and field studies with insects on Vicia sepium. Oecologia 122:129-137.
69
Project ID: 01LC0020 (BIOPLEX Subproject 2)
01.10.2000-30.09.2003
BIODIVERSITY
AND
SPATIAL
COMPLEXITY
IN
AGRICULTURAL
LANDSCAPES UNDER GLOBAL CHANGE (BIOPLEX) - AGROECOLOGY
Carsten Thies, Judith Schmid and Teja Tscharntke
Georg-August-University, Agroecology, Waldweg 26, 37073 Goettingen, Germany
Contact: c.thies@uaoe.gwdg.de; ttschar@gwdg.de; http://www.gwdg.de/~uaoe
Key words:
biodiversity, ecological functions, landscape structure, landscape complexity, biological
control, trophic interactions, top down, bottom up, scale dependence, wheat, cereal aphids,
parasitoids.
Abstract:
The Agroecology group investigates the influence of landscape structure and farming
management on biodiversity and functioning of ecological processes in agricultural
landscapes of northern and middle Germany. The analyses focus on species diversity and
species interactions in wheatcrop fields and grasslands. In wheat fields, we address factors
that affect the presence, abundance and species richness of natural enemies of cereal aphids
(and weeds) as well as the role of pathogens (with project group 3: Phytopathology) and
below- and above-ground processes (with project group 4: Soil Ecology) in relation to
landscape complexity. We evaluate the ability of ”natural enemies” of pests (and weeds) to
act as ”biocontrol agents”, i.e. species which suppress the damage caused to crops by pests
below an economic threshold. In grasslands we focus on the combined effects of pollinators
and seed predators on plant’s seed set. The analysis of the spatio-temporal variability of these
counter-rotating processes will give a deeper insight to multitrophic interactions of important
functional groups in terrestrial ecosystems, and to modeling biodiversity (with project group
1: Modeling and Geostatistics) at a range of spatial scales upscaling from microsites, via the
field and farm scale to landscapes and the global context.
Results:
Since there is an increasing recognitation that community structure, species abundance and
biotic interactions may depend on scales much larger than a single habitat, a landscape
perspective considering spatial arrangement, connectivity and environment of habitats may be
undispensable in determining local ecological pattern and processes (Kareiva 1990, Kruess &
Tscharntke 1995, Roland und Taylor 1997, Thies & Tscharntke 1999). However, landscape
context does not influence all species equally, and interacting communities are made up of
species with very different spatial strategies. Species show specific responses to landscape
structure facilitating or impeding movement among habitat patches, and with a specific
perception of spatial scale of landscape complexity (With et al. 1999). Here, we present initial
results from herbivore-parasitoid interaction in wheat fields (Triticum aestivum) located in 46
landscapes differing in structural complexity. We analysed the most important crop pest on
wheat in Germany, the cereal aphid Sitobion avenae (Homoptera: Aphididae), and its
mortality due to parasitoids (Hymenoptera parasitica). The abundance of the aphid S. avenae
as well as its mortality due to parasitism was correlated with the perimeter/area ratio (P/A).
The P/A ratio serves as a useful index of shape complexity in a landscape. For a given area, a
high P/A ratio indicates a complex or elongated boundary shape, and a low P/A ratio indicates
a more compact and simple shape.
70
The abundance of aphid S. avenae in wheat field increased (slightly) as the P/A ratio
increased from 0.5 to 1.5, but decreased (slightly) as the P/A ratio increased from 1.5 to 3
(Fig. 1A). Mortality of S. avenae due to parasitoids increased distinctly, without any evidence
of a threshold, from values of 0% to 50% as the P/A ratio increased from 0.5 to 3 (Fig. 1B).
Our results clearly show, that the efficiency of natural enemies, i.e. their potential to act as
”biocontrol agents”, is generally enhanced in complex landscapes, obviously leading to a
tendency of lower pest abundance in landscapes with a P/A ratio >1.5-2. The is corresponding
to Hawkins & Cornell (1994). They predicted success in classical biological control only
above a threshold value of 32-36%, which is the case in our study system above a P/A ratio
>1.5-2.
Sitobion avenae
Sitobion avenae
4096
Parasitism (%)
1024
Individuals/m²
79
A
256
64
16
4
0
0,5
1
1,5
2
2,5
Perimeter/Area-Ratio
B
50
35
21
10
3
0
3
Lower Saxony
Middle Hesse
65
0,5
1
1,5
2
2,5
3
Perimeter/Area-Ratio
Figure 1: Herbivor-parasitoid interactions in wheat fields (Triticum aestivum) in landscapes of
different complexity (N= 46) located in Lower Saxony and middle Hesse (Germany). (A) Abundance
of the crop pest Sitobion avenae in relation to the perimeter/area ratio (P/A). Y= 1.55 + 4.19 × P/A –
1.26 × P/A². F= 2.74, PModel = 0.075, PP/A= 0.024, PP/A²= 0.028, N= 46, R²= 11%. (B) Mortality rate of
Sitobion avenae due to parasitim in relation to the perimeter/area ratio (P/A). Y= 3.54 + 15.54 × P/A.
F= 12.31, P= 0.001, N= 46, R²= 22%. Each data point is based on four subsamples per field
(inspection of 4 × 20 shoots per field for aphids and aphid mummies in June). Statistical analyses
comprised multiple linear and non-linear regressions with two independent landscape metrics, the P/A
ratio and the crop area [%] per landscape (see BIOPLEX project group 1 for further explanation) and
host plant density (shoots/m²; Fig. 1A) or host density (aphids/m²; Fig. 1B), respectively. Only P/A
ratio arised as factor of explanation.
References:
Hawkins, B. A. and Cornell, H. V. 1994. Maximum parasitism raten and successful biological control.
Science: 262: 1886.
Kareiva, P. 1990. Population dynamics in spatial complex environments: theory and data. - Phil.
Trans. R. Soc. Lond. B 330: 175-190.
Kruess, A. and Tscharntke, T. 1994. Habitat fragmentation, species loss, and biological control. Science 264: 1581-1584.
Roland , J. and Taylor, P. D. 1997. Insect parasitoid species respond to forest structure at different
spatial scales. - Nature 386: 710-713.
Thies, C. and Tscharntke, T. 1999. Landscape structure and biological control in agroecosystems. Science 285: 893-895.
With, K. A., Cadaret, S. J. and Davis, C. 1999. Movement responses to patch structure in experimental
fractal landscapes. - Ecology 80: 1340-1353.
71
Project ID: 01LC0020 (BIOPLEX Subproject 3)
01.10.2000-30.09.2003
BIODIVERSITY
AND
SPATIAL
COMPLEXITY
IN
AGRICULTURAL
LANDSCAPES UNDER GLOBAL CHANGE (BIOPLEX) - PHYTOPATHOLOGY
Lars Reimer, Julia Morzfeld, Stefan Vidal
Institute of Plant Pathology and Plant Protection, Goettingen University, Grisebachstr 6, D-37077
Goettingen
svidal@gwdg.de; http://www.uni-goettingen.de
Key words:
Sitobion avenae, DNA-Fingerprinting, Septoria tritici, genetic variability,
landscape-structure, agricultural utilization
Abstract:
The BIOPLEX subproject Phytopathology aims at evaluating the influence of landscape
structure on population processes at the genetic level. Using cereal aphid species and plant
pathogens on winter wheat as targets we investigate the genetic diversity of these species
within and between fields at a local and a regional scale to evaluate the influence of the
structural and agronomical diversity of the surrounding landscape at different spatial scales.
The project started with the year 2001. Sampling methods were determined in cooperation with
other subprojects. Samples were taken two or three times, respectively (according to specific
developmental stages of the wheat) in up to 40 fields in the environment of Goettingen and in the
Lahn-Dill-Bergland. The wheat fields were selected by means of GIS-Analysis. During sampling
dates additional plants were collected to determine biomass as a covariate. On each field in the
environment of Goettingen small areas were excluded from insecticide application for aphid
sampling. All farmers were questioned on information concerning agricultural cultivation
treatments (tillage strategies, application of fungicides, fertilization, cultivars and crop rotation).
Insects (L. Reimer)
About 30 to 100 cereal aphids, predominantly Sitobion avenae, were collected during two
sampling dates from 36 field sites in the region around Giessen and Goettingen. Sweepnet
collections were performed along a transect, the length of which was adapted to field size and
the number of aphids available. Aphid captures were located at least one meter apart from
each other to collect as many clones within one field as possible. >From each sweepnet
capture only one nonparasitised individual, identified to species level, was taken for further
DNA analysis. Indentifications were verified by Dr. T. Thieme (BTL GmbH Sagerheide).
Samples were stored either deep frozen at –20 °C or in 99 % Ethanol. For the analysis of
microsatellite length polymorphism (Sunnucks et al. (1)) six primers are actually available.
Between 91 and 127 individuals from five field sites of the Goettingen region were screened
for five primers and 5 to 25 different alleles were found per locus. The number of alleles so
far found is as high or even higher than reported in papers that used a higher number of
individuals. The distribution of distinct alleles within a site appears clumped with regard to
certain alleles. An example on the allele frequencies at one locus is given in Tab. 1. There are
predominant alleles and also rare ones. The allelic distribution seems to be similar at different
field sites, but some shifts in allele frequencies do occur (field site 2/1, Tab. 1). The
genotypes, defined as individuals sharing the same alleles at all loci, show a high diversity.
Only two genotypes were found more than once in the different field sites. These genotypes
are homozygous at three or four loci and consist mainly of common alleles.
72
Table 1: Allele frequencies of Sitobion avenae microsatellites at one locus. 125 Individuals were
screened and 246 alleles (N) were scored in total.
Allele size in basepairs Field site 1/1 Field site 2/1 Field site 9/1 Field site 12/1 Field site 18/1
N = 62
N = 26
N = 62
N = 48
N = 48
185
0.79
0.42
0.75
0.75
0.75
186
0.13
0.27
0.11
0.17
0.23
188
0.02
0.04
0.03
0.02
0.00
189
0.00
0.04
0.00
0.00
0.00
190
0.05
0.23
0.11
0.04
0.00
191
0.02
0.00
0.00
0.02
0.02
Additionally, a new primer will be included in further investigations, because certain primers
developed from S. miscanthi failed to amplify in most cases and two or three PCR runs were
necessary to obtain positive results.
The data on the genetic diversity will be analysed for differences between field sites in the
Lahn-Dill Bergland and Goettingen. Moreover, these data will be compared with subsamples
from Northern Germany and Hungary and also with published data from France and England.
The microsatellite data will be also analysed for deviations from the Hardy-Weinberg
equilibrium, gene-flow and differences between locations. The data on the population
dynamics of the cereal aphids with regard to their spatial and temporal distribution will be
covered by other project partners.
Plant pathogenic fungi (J. Morzfeld)
This study aims at analysing the influence of the landscape-structure on the incidence,
distribution and frequency of leaf pathogenic fungi on wheat. We will especially analyse the
impact of landscape structure on the genetic variability of Septoria tritici populations, due to
his widespread occurrence. According to the prevailing hypothesis, the distribution and
damage of leaf-pathogenic fungi is influenced by measures of agricultural cultivation.
Strategies of tillage, application of fungicides, fertilization, cultivars used or crop rotation are
regarded of major importance among these measures. An alternative hypothesis states that
plant pathogens are less abundant in natural ecosystems compared to agricultural ecosystems.
By incorporating both agronomic traits and landscape structure into the analysis of the
population genetics of fungi on winter wheat we will be able to separate the importance of
either agricultural measures or environmental heterogeneity on fungal biodiversity.
To determine the incidence and damage of plant pathogenic fungi on winter wheat we
sampled 50 plants on 28 field sites at three different sampling dates. One leaf of each plant (F2 or F-1) was qualitatively and quantitatively checked for the following fungal species:
Septoria tritici, Septoria nodorum, Drechslera tritici-repentis, Blumeria graminis var. tritici,
Puccinia striiformis and Puccinia recondita. After visual inspection leaves were collected,
dried in the laboratory and then stored at –40 °C. Septoria tritici was the most frequent and
widely distributed fungi; it was found in both regions in each field. The five other plant
pathogenic fungi were distributed in an irregular pattern.
The necrotrophic fungus Septoria tritici will be isolated from the stored leaves and grown in
vitro (Schnieder et al. (2)). The genetic variability of this fungus will be analysed by a
fingerprinting-method (MP-PCR: microsatellite-primed-PCR; see Czembor et al. (3)). By
means of experiments with different Septoria-Isolates (Collection of the institute) ahead of
screening DNA-Isolates, experimental conditions, primers for the fingerprinting method,
PCR- and other laboratory routines were optimised.
References:
1. Sunnucks, P., England, P. R., Taylor, A. C. and Hales, D., Genetics 144, 1996, 747-756
2. Schnieder, F., Koch, G., Jung, C. and Verreet, J.-A., Europ. J. of Plant Pathology 107, 2001,
285-290
73
3. Czembor, P. C. and Arseniuk, E., J. Phytopathology 147, 1999, 539-546
Project ID: 01LC0020 (BIOPLEX Subproject 4)
1.10.2000 – 30.09.2003
BIODIVERSITY
AND
SPATIAL
COMPLEXITY
IN
AGRICULTURAL
LANDSCAPES UNDER GLOBAL CHANGE (BIOPLEX) - Soil BIOTA
J. Dauber, T. Purtauf and V. Wolters
Department of Animal Ecology, Justus-Liebig-University, Heinrich-Buff-Ring 26-32 (IFZ), D35392 Giessen
jens.dauber@allzool.bio.uni-giessen.de, http://www.uni-giessen.de/bioplex
Key words: landscape complexity, soil fauna, ground beetles, ants, wheat, grassland
Abstract:
The character of Central European landscapes is shaped by human land-use, especially
agriculture. The continuous expansion of agriculture over the last centuries created open
landscapes with new habitats and led to an increase of biodiversity. Therefore biodiversity of
Central Europe depends on agriculture. However, industrialisation of agricultural
production and intensification of land-use over the last decades led to enormous habitat
destruction and thereby to a decrease in biodiversity. The question arising from this
development is: What kind of agricultural landscape do we need to sustain high levels of
biodiversity? The multidisciplinary project BIOPLEX approaches this question by analysing
the relationship between biodiversity and landscape complexity at different spatial scales.
The studies focus on wheat fields and managed grasslands in two agricultural regions of
Germany (Lahn-Dill-Bergland, Hesse and Southern Lower Saxony). The subproject Soil
Ecology aims at: (i) recording the species richness of soil living macroinvertebrates (ground
beetles, rove beetles, woodlice, diplopods) and soil engineers (ants), (ii) analysing the effect
of epigaeic predatory arthropods on cereal aphids in winter wheat, and (iii) quantifying the
functional role of decomposers depending on landscape complexity. A comparison between
the data derived from these analyses with data on phytophagous insects, plant pathogens and
vegetation will reveal some of the relationships between above- and belowground
biodiversity.
Results:
Sustainable management of biodiversity at the landscape level urgently needs simple
indicators of biodiversity. Landscape structure affects ecological processes and species
richness (Wiens et al. 1993). Landscape pattern descriptors, which correspond to spatial
complexity and to varying amounts of land-use types might serve as useful tools to explain
differences in species richness across landscapes (Miller et al. 1997). Field experiments and
surveys on this topic rely on representative selection of study sites by landscape analyses,
taking into calculation the landscape features of interest for the respective survey. Ideally,
there is a small set of metrics, which span the important dimensions of landscape structure
(Riitters el al. 1995). The selection of study sites in a gradient of landscape complexity is
shown in Behrens et al. 2001. Base maps used for the study site selection were a DEM
(Digital Elevation Model) and the official German land-use database called ATKIS
(Amtliches topographisch-kartographisches Informationssystem). The decision to use these
base maps was taken on base of the results of a preliminary study out of which some of the
results are presented here:
74
variance explanation
Species richness of staphylinids, carabids and ants was recorded in a small-scale mosaic
landscape. In a complete factorial design we sampled the major land-use types of the open
landscape: arable land, managed grassland and fallow land. The study sites were further
characterised by different environmental conditions like soil type (dry or moist) and aspect
(south or north oriented). We tested the effects of land-use, aspect and soil type by a three
factorial ANOVA.
A total of 153 staphylinid species, 100 carabid species and 27 ant species were found. Each
land-use type contributed to total species richness of all three taxa at the landscape level.
Species richness significantly differed between the land-use types in a taxon-specific way.
Mean species richness of staphylinids and ants was highest in fallow land whereas species
richness of carabids was highest in arable land. Among the environmental parameters aspect
had a significant effect on species richness of staphylinids and ants with staphylinids showing
higher species richness at north oriented sites and ants at south oriented sites. Soil type had a
significant effect on carabids and ants. Species richness of both taxa was higher on dry soils.
Land-use was the factor explaining the highest proportion of variance of staphylinids and
carabids (Fig. 1).
Aspect had the highest
100%
variance explanation
for ants, i.e. a highly
unexplained
80%
thermophilous group.
other interactions
land use x soil type
Therefore hot spots of
**
60%
soil type
ant diversity in a
*
**
aspect
landscape are to be
*
land use
***
expected at south
*
40%
oriented slopes. Non***
significant
inter***
20%
actions indicate that
*
environmental para0%
meters affect species
staphylinids
carabids
ants
richness of all taxa
independent from the
Fig. 1: Variance explanation of the factors land-use, aspect and soil type and
their interactions for species richness of staphylinids, carabids and ants as
land-use. Only species
derived from a three factorial ANOVA. P-levels: * < 0.05, ** < 0.01, *** <
richness of staphyli0.001.
nids was not affected
by soil type per se, but soil type had a modifying influence on the effect of land-use as
indicated by the significant interaction between these two factors. Nevertheless, there still is a
high percentage of variance unexplained by the factors considered so far. We expect
landscape complexity to be a further significant landscape variable affecting species richness
of soil macroinvertebrates.
References:
Behrens, Th., Purtauf, T., Wolters, V., Köhler, W. and Dauber, J., 2001. Study site selection and
gradient detection in complex landscapes using an automated patch detection tool (PaDS). In:
Belward, A., Binaghi, E., Brivio, P.A., Lanzarone, G.A. and Tosi, G. (Eds.) Geo-Spatial
Knowledge Processing for Natural Resource Management, Daverio, 217-220.
Miller, J.N., Brooks, R.P. and Croonquist, M.J., 1997. Effects of landscape patterns on biotic
communities. Landscape Ecology, 12, 137-153
Riitters, K.H., O’Neill, R.V., Hunsaker, C.T., Wickham, J.D., Yankee, D.H., Timmins, S.P. and
Jackson, B.L., 1995. A factor analysis of landscape pattern and structure metrics. Landscape
Ecology, 10, 23-39
Wiens, J.A., Stenseth, N.C., Van Horne, B. and Ims, R.A., 1993. Ecological mechanisms and
landscape ecology. Oikos, 66, 369-380
75
Project ID: 01LC0020 (BIOPLEX Subproject 5)
01.11.2000 – 31.10.2003
BIODIVERSITY
AND
SPATIAL
COMPLEXITY
IN
AGRICULTURAL
LANDSCAPES
UNDER
GLOBAL
CHANGE
(BIOPLEX)
SOCIAL-ECONOMIC EVALUATION AND APPRAISAL WITH RESPECT TO
BIODIVERSITY IN AGRARIAN LANDSCAPES
Elke Bertke, Katja Diedrich, Anke Fischer, Sonja Katerina Hespelt, Christoph Tute,
Dr. Bärbel Gerowitt, Prof. Dr. Johannes Isselstein, Prof. Dr. Rainer Marggraf
Research Centre for Agriculture and the Environment
Faculty of Agriculture, Georg-August-University of Göttingen,
Am Vogelsang 6, 37075 D-Göttingen
bgerowi1@gwdg.de; http://www.uni-goettingen.de/zlu (BIOPLEX - Social-economic concepts)
Key words: system of payment, ecological services, information, contingent valuation method,
biodiversity, production value, agriculture
Abstract:
The subproject V is structured into three parts. The part "Result-Oriented System of Payment for
Ecological Services“ deals with the development of an economic system of payment for ecological
services provided by farmers. These services will be measured by ecological goods arising from
plant biodiversity. The part of the project that deals with “The Influence of Information on the
Economic Valuation of Natural Resources” has two objectives. On the one hand, it aims at an
economic evaluation of ecological goods defined in the first part of the subproject (s. a.). On the
other hand, different influences of information that occur during the process of evaluation in a
contingent valuation, are investigated in an interdisciplinary approach. Another part of the
project concerns „The Impact of Certain Components of Biodiversity on the Production Value in
Agriculture“. This aims at assessing the utility certain components of biodiversity and,
simultaneously, ecological goods have got for agriculture.
Results:
“Result-Oriented System of Payment for Ecological Services“
Ecological services performed by the farming community are positive external effects. At present
some of these ecological services are financed under agri-environmental programs or agrienvironmental contracting. In these programs the farmers get paid for acting according to special
cropping instructions, e.g. dispensing pesticide use.
In theory, such services could be increased if the financial incentives on offer are improved. Our
research project focuses on developing a concept of a regional market and result-oriented system
of payment for ecological services provided by farmers. The results of ecological services are
ecological goods. Goods arising from plant biodiversity can be considered to be those most
suitable for a result-oriented system of payment. These ecological goods should be supplied by
the farmers voluntarily and without set regulations. Furthermore, the public demand for ecological
goods should be represented by a regional advisory board in the sense of the European
subsidiarity principle. This advisory board has to award public contracts for the production of
ecological goods.
In this way, environmental programs could be made more flexible and the farmers would receive
financial incentives to find innovative solutions for an environmentally compatible management.
76
The concept further takes into account the preferences of the local population for ecological goods
and for the allocation of public funds.
In our transdisciplinary research work we develop this result-oriented system of payment for a
representative region in the south of Lower Saxony (County Northeim) and check the possibilities
of its application in practice with the relevant institutions of that county. The members of the
regional advisory board represent the administrations of agriculture and environment protection,
the local affairs, and the associations of agriculture, conservation, and land owners. Up to now we
defined regional specific ecological goods associated with grassland use, we worked out
management proposals to obtain these ecological goods, and calculated the costs. The award
procedures for public orders, complying with the law, were examined with regard to their
transferability on a market for ecological goods.
“The Influence of Information on the Economic Valuation of Natural Resources“
Non-market common goods, such as ecological commodities, cannot be evaluated by their market
price. To estimate their monetary value, ecological economists have developed several methods of
indirect and direct evaluation, for example, the contingent valuation method (CVM), which elicits
willingness to pay-values by means of an interview.
In this study, CVM will be applied to estimate the willingness to pay of the citizens living in the
representative region, county Northeim (s. a.), for certain ecological goods (s. a.), such as
hedgerows in agrarian landscapes. At the same time, the impacts of information imparting and
information processing on the valuation of the ecological good in question during an interview
situation are investigated. For this purpose, in a first step the findings of other disciplines have
been summarised and analysed, based on a common theoretical framework, the Theory of
Planned Behaviour. In a second step, hypotheses about information processing and decision
making of respondents in CV situations have been derived from this analysis. They will be tested
in a study orientated by findings and methods of social and consumer psychology.
“The Impact of Certain Components of Biodiversity on the Production Value in Agriculture“
Local explorations of natural sciences in this field have shown that certain components of
biodiversity may be economically important for agricultural production. With the aid of an expert
questionnaire, these sporadic results shall be tested by their relevance in different agricultural
landscapes, and the capability of generalising them be assessed, respectively.
So far, the recent results presented in literature have been explored and summarised in causes and
effects. As the analysis will be conducted according to the Delphi method, the summaries will
now be used for developing a standardised questionnaire. With the aid of the Delphi-Questioning,
the opinion of a group of experts will be ascertained and structured. As the experts stay
anonymous in this kind of questionnaire, biases in the results which may result from the opinions
of too strong characters can be avoided. This is, however, often the case in group discussions.
Apart from that, various data bases have been checked to find persons with expertise in this field
of research. This is crucial as the quality of the results depends substantially on a good choice of
experts.
77
Project ID: 01LC0021 (Subproject 1)
1.1.2000 - 31.12.2003
BIODIVERSITY OF NITROGEN-FIXING MICROORGANISMS
Lei Zhang,1 Torsten Thünen,1 Thomas Hurek,2 and Barbara Reinhold-Hurek1
1
Laboratorium für Allgemeine Mikrobiologie, 2 Kooperationslabor des Max-Planck-Instituts
für marine Mikrobiologie Bremen, Universität Bremen FB 2, Leobener Strasse NW2, D28359 Bremen
breinhold@uni-bremen.de, thurek@uni-bremen.de
Key words: Uncultured bacteria, nitrogenase genes, rice roots, DNA-microarrays, mRNA
detection
Abstract:
The project aims to analyze the biodiversity of nitrogen-fixing prokaryotes by molecular and
classical techniques. During this project, the molecular methods to address questions of
terrestrial biodiversity are developed and established: Development of monitoring systems for
fast, reliable assessment of the biodiversity of diazotrophic bacteria in association with plant
roots, and for analysis of the dynamics of their population structure and their key activities
(expression of nitrogenase genes). Protocols were developed to quantify bacterial nifH
(nitrogenase gene) mRNA extracted from roots, and clone libraries of bacterial nifH genes
completed, demonstrating that the majority of rice root-associated diazotrophs has not yet
been cultured. One of the major achievements is the development of a DNA-microarray which
can specifically detect and identify a large variety of specific nitrogenase gene fragments
amplified by PCR, without the necessity for clone libraries.
Results:
The diversity of prokaryotic organisms is classically estimated by attempts to cultivate them and
by characterization of the isolates. However, during the last decade DNA- or RNA- based
techniques led to the conclusion that the organismal diversity is much higher than estimated by
these classical techniques: the majority of bacteria present in a given habitat has in most cases not
been cultured as yet, and often these bacteria are phylogenetically very distant to known,
cultivated bacterial strains [1]. This is also the case for root-associated bacteria [2, 3]. In order to
assess the biodiversity and the population structure in a given habitat, their changes in response to
environmental changes, and also in order to understand functions of microorganisms in the
habitat, culture-independent methods are indispensable.
The most widely used target molecules for molecular ecological studies are ribosomal rRNA
genes [1, 4]. This allows an estimation of the biodiversity, but does –in most cases- not permit to
link the results to physiological functions of the bacteria. Functions however may be analyzed
when key genes for certain metabolic processes are used as target genes. In the global nitrogen
cycle, biological fixation of atmospheric nitrogen is an important microbial activity. The
availability of combined nitrogen affects plant productivity and the structure of the plant
community [5]. Increasing anthropogenic activities result in an increased contamination of the
atmosphere by nitrogenous compounds, and in deposition of nitrogen from the atmosphere on
soils, even without application of fertilizer [6]. N-deposits are also likely to affect the population
of diazotrophs. One of the structural genes of nitrogenase, nifH, is particularly suitable for
phylogenetic analysis [3, 7].
We established protocols which allow to retrieve bacterial mRNA from soil-grown rice roots. The
degradation of RNA was sufficiently inhibited for the detection of nifH mRNA by reverse
transcriptase (RT) analysis [8]. Template dilution allowed a quantitative analysis of transcript
78
abundance, the application to different rice varieties revealing significant differences. In clone
libraries constructed from these amplification products, most sequences analyzed were only
distantly related to nitrogenase genes of known, cultured bacteria. Thus, up to now uncultured
diazotrophs appear to be predominantly active in rice roots.
A new method for the assessment of biodiversity is being developed which may allow the specific
detection and quantification of a large numbers of different nifH mRNA fragments without
construction and analysis of clone libraries. DNA microarrays or DNA-chips are widely used for
highly parallel and high througput analyses of gene expression in genome research.
Oligonucleotides or DNA-fragments which are complementary to target genes are linked to
carriers such as membranes or glass slides, and used as baits for hybridization with labeled DNA
or RNA under study. This methodology is currently adopted by us to construct a „phylo-chip“, a
glass microarray for specific detection of the diversity of nif genes. Our database of nifH genes
from cultured diazotophs and fragments retrieved from environmental samples was used to design
short oligonucleotides which are specific to single genes or groups of genes with at least 1-2
mismatches to each other. The oligonucleotides were attached to activated glass slides via aminolinks at specific postions, hybridized to fluorescence (Cy-5)-labeled amplification products of a
nifH-directed PCR, and the hybridizations quantified with a microarray scanner. The
oligonucleotide design was optimized with respect to the position of hybridization within the
fragment and other factors. Using genes of the Azoarcus group as examples, a hybridization
protocol was developed in which one mismatch was sufficient to result in a specific hybridization
signal. Thus from mixtures of DNA of different bacteria, their nitrogenases were specifically
identified. The next steps of development will be the detection of root-associated nifH fragments
using the phylo-chip, and the enlargement of the number of oligonucleotide baits per chip to cover
most of the nitrogenase gene diversity.
References:
1. Pace, N.R. (1997) A molecular view of the microbial diversity and the biosphere. Science 276: 734-740.
2. Engelhard, M., Hurek, T. and Reinhold-Hurek, B. (2000) Preferential occurrence of diazotrophic
endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with
modern races. Environ. Microbiol. 2: 131-141.
3. Ueda, T., Suga, Y., Yahiro, N. and Matsuguchi, T. (1995b) Remarkable N2-fixing bacterial diversity
detected in rice roots by molecular evolutionary analysis of nifH gene sequences. J. Bacteriol. 177:
1414-1417.
4. Amann, R.I., Ludwig, W. and Schleifer, K.H. (1995) Phylogenetic identification and in situ detection of
individual microbial cells without cultivation. Microbiol. Rev. 59: 143-169.
5. Bakker, J.P. and Berendse, F. (1999) Constraints in the restoration of ecological diversity in grassland
and heathland communities. TREE 14: 63-68.
6. Vitousek, P.M., Aber, J.D., Howarth, R.W., Likens, G.E., Matson, P.A., Schindler, D.W., Schlesinger,
W.H. and Tilman, D.G. (1997) Human alteration of the global nitrogen cycle: sources and
consequences. Ecol. Appl. 7:737-750.
7. Hurek, T., Egener, T. and Reinhold-Hurek, B. (1997) Divergence in nitrogenases of Azoarcus spp.,
Proteobacteria of the ß-subclass. J. Bacteriol. 179: 4172-4178.
8. Hurek, T., Handley, L., Reinhold-Hurek , B. and Piché, Y. (2001) Azoarcus grass endophytes function in
nitrogen fixation by contributing fixed nitrogen to the plant in an unculturable state. Mol. PlantMicrob. Interact.: in press.
79
Project ID: 01LC0021 (Subproject 2)
1.1.2001 – 31.12.2001
BIODIVERSITY OF NITRIFYING AND SULFATE-REDUCING PROKARYOTES
A. Loy, J. Adamczyk, A. Lehner, and M. Wagner
Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, 85350
Freising
wagner@mikro.biologie.tu.muenchen.de; www.microbial-ecology.de
Key words: Nitrification, sulfate-reduction, 16S rDNA, DNA-microarray, ammoniamonooxygenase gene amoA, dissimilatory sulfite reductase genes dsrA/B
Abstract:
Nitrifying and sulfate-reducing prokaryotes catalyze key steps in the nitrogen and carbon-cycle,
respectively, and are thus of primary importance for the ecology of most ecosystems. The project
aims to develop and adapt methods suitable for cultivation-independent investigations of the
diversity and function of nitrifying (ammonia-oxidizing and nitrite-oxidizing) and sulfate-reducing
prokaryotes living in the rhizosphere. For both groups of microorganisms 16S rDNA as well as
suitable functional genes are used as marker molecules (1, 2 ,4 ,6). Based on encompassing data
sets of these marker molecules, oligonucleotide microarrays for highly parallel, high throughput
screening of these prokaryotes in environmental samples were developed and initially applied to
investigate samples from a Sphagnum bog. These techniques will be supplemented by optimizing
the combination of FISH and microautoradiography (3) for its use in rhizosphere and soil in
order to determine the links between the availability of specific substrates (for example by plant
root exudation) and the microbial biodiversity. These investigations will provide novel insights
into diversity and activity patterns of soil and rhizosphere microorganisms and into interactions
between plants and microorganisms.
Results:
During the first project phase we have established encompassing 16S rDNA data sets for
nitrifying and sulfate-reducing prokaryotes. Based on this information, microarrays for nitrifying
and sulfate-reducing bacteria were designed. These arrays, which consist of several hundred
oligonucleotide probes, were thoroughly evaluated under different hybridization conditions using
reference sets of suitable pure cultures and are now ready for application in environmental
diversity surveys. Furthermore, we started to design and evaluate DNA microarrays for ammoniaoxidizing and sulfate-reducing prokaryotes by exploiting the genes encoding the ammoniamonooxygenase (amoA) and the dissimilatory sulfite-reductase (dsrA/B), respectively, as target
molecules.
The developed microarrays are now used to investigate the diversity of nitrifiers and sulfatereducers in a variety of different rhizosphere samples. In collaboration with Prof. Drake and Dr.
Küsel (Lehrstuhl für ökologische Mikrobiologie, Bayreuth) we also began to investigate the
community composition of sulfate-reducing prokaryotes in different depths of a Sphagnum-bog, a
system for which sulfate-reduction rates comparable to those of marine systems have been
reported (for example 5).
80
References:
1. Daims, H., Nielsen, J.L., Nielsen, P.H., Schleifer, K.H., and Wagner, M., Appl. Environ. Microbiol.,
2001, 67,5273-5284.
2. Klein, M., Friedrich, M., Roger, A.J., Hugenholtz, P., Fishbain, S., Abicht, H., Blackall, L.L., Stahl,
D.A., and Wagner, M., J. Bacteriol., 2001, 183,6028-6035.
3. Lee, N., Nielsen, P. H., Andreasen, K. H., Juretschko, S., Nielsen, J. L., Schleifer, K. H., and Wagner,
M., Appl. Environ. Microbiol, 1999, 65,1289-1297.
4. Purkhold, U., Pommerening-Röser, A., Juretschko, S., Schmid, M.C., Koops H.P., and Wagner, M.
Appl. Environ. Microbiol., 2000, 66, 5368–5382.
5. Urban, N.R., Eisenreich, S.J., and Grigal, D.F., Biogeochem., 1989 7,81-90.
6. Wagner, M, Flax, J. L., Brusseau, G. A., and Stahl, D. A., J. Bacteriol., 1998, 180, 2975-2982.
81
Project ID: 01LC0021 (Subproject 3)
1.1.2000 – 31.12.2003
BIODIVERSITY OF METHANOTROPHIC AND NITRATE-REDUCING BACTERIA
G. Braker, P. Dunfield, H.P. Horz and R. Conrad
Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., 35043 Marburg
Conrad@mailer.uni-marburg.de; http://www.uni-marburg.de/mpi
Key words: methane oxidation, nitrate reduction, denitrification, nitric oxide, nitrous oxide
Abstract:
We investigate microbial processes in soils. The research focuses on the question which processes
on a microscopic scale are catalyzed by which microorganisms. Besides the functional diversity
in soil with respect to processes, there is an even higher structural diversity with respect to the
microorganisms that are responsible for the functions. The extent of the soil microbial diversity is
completely unknown. In particular, it is unclear how much microbial diversity is required to
maintain soil functions. Within BIOLOG we study the biodiversity of methanotrophic bacteria and
nitrate-reducing bacteria in relation to their functioning in soil environments. Biodiversity is
studied by targeting both the 16S rRNA genes and the structural genes of key enzymes. Here, we
targeted the pmoA genes of methanotrophs coding for the particulate methane monooxygenase,
and the nirK/nirS and norB genes of denitrifiers coding for nitrite reductase and nitric oxide
reductase, respectively.
Results:
We have studied the diversity of the methanotrophic microbial community (i) in rice field soils
from different geographic regions 1 ; and (ii) in a meadow soil with seasonally changing water
table2 . Furthermore, (iii) the genetic diversity of denitrifiers was explored by PCR amplification of
nirK/nirS3 and norB4 genes from pure cultures and environmental samples.
(i) Methanotrophic bacteria play a crucial role in regulating the emission of CH4 from rice fields
into the atmosphere. We investigated the CH4 oxidation activity together with the diversity of
methanotrophic bacteria in ten rice field soils from different geographic locations. Upon
incubation of aerated soil slurries under 7% CH4 , rates of CH4 oxidation increased after a lag
phase and reached values of 3-10 µmol d-1g-dw-1 soil. The methanotrophic community was
assayed by retrieval of the pmoA gene which encodes the a subunit of the particulate methane
monooxygenase. After extraction of DNA from actively CH4-oxidizing soil samples and PCRamplification of the pmoA, the community was analyzed by Denaturant Gradient Gel
Electrophoresis (DGGE) and Terminal Restriction Fragment Length Polymorphism (T-RFLP).
DGGE bands were excised, the pmoA re-amplified, sequenced and the encoded amino acid
sequence comparatively analyzed by phylogenetic treeing. The analyses allowed the detection of
pmoA sequences related to the following methanotrophic genera: the type-I methanotrophs
Methylobacter, Methylomicrobium, Methylococcus and Methylocaldum, and the type-II
methanotrophs Methylocystis and Methylosinus. T-RFLP analysis detected a similar diversity, but
type-II pmoA more frequently than DGGE. All soils but one contained type-II in addition to type-I
methanotrophs. Type-I Methylomonas was not detected at all. Different combinations of
methanotrophic genera were detected in the different soils. However, there was no obvious
geographic pattern of the distribution of methanotrophs.
82
(ii) Methane oxidation activity and the structure of the methane-oxidising microbial community in
a wet meadow soil in Germany were investigated using biogeochemical, culturing, and molecular
fingerprinting techniques. Most-probable-number counting of methane-oxidising bacteria
followed by isolation and characterisation of strains from the highest positive dilution steps
suggested that the numerically dominant member of the methane-oxidising community was a
Methylocystis strain (≅106 cells g-1 d.w. soil). Calculations based on kinetic data support the
conclusion that this organism could account for most of the observed methane oxidation activity
in the soil. DNA extraction directly from soil and PCR amplification of the pmoA gene also
recovered Methylocystis-like sequences. However, molecular community fingerprinting analyses
also revealed a more diverse and dynamic picture of the methane-oxidising community, and there
were large variations across samples. Retrieved pmoA sequences included, besides Methylocystis
sequences, others related to the genera Methylomicrobium and Methylocapsa, and a novel lineage.
A combination of classical microbiological methods and molecular fingerprinting therefore gave a
better picture of the microbial community in this meadow soil than either method alone, i.e. the
molecular methods revealed a higher species richness and uncovered the presence of
Methylocapsa, but failed to demonstrate the consistently high abundance of Methylocystis, and
occasionally even failed to detect this organism despite its abundance.
(iii) The genes nirK and nirS encode two structurally different but functionally equivalent
enzymes occurring within the same genus but not within the same strain of a denitrifier. Two
classes of norB genes exist with low levels of identity: one type with an adjacent norC gene and a
second type with a N-terminal extension instead. Amplification of nirK, nirS, and norB was
successful from pure cultures within the Proteobacteria. Cloning of nir genes from environmental
samples revealed gene clusters distinct from those of known denitrifiers suggesting novel lineages
of yet uncultivated denitrifiers, probably also within the Proteobacteria. Gene clusters were
specific for the habitat from which these clones were obtained. Phylogenetic analysis of norB
grouped genes according to two classes; within the norCB class, norB genes from isolates
grouped together with clones suggesting that most of these environmental sequences might be
derived from Proteobacteria. Within the second class, genes from all known isolates except one
were grouped in one cluster, clones were unrelated and scattered. Interestingly, in contrast to nir
genes, which clustered strongly according to the environment from which they were derived, this
was not observed for norB. With our current knowledge, the origin of novel sequences and their
distribution patterns in the environment remain obscure. Future research will focus on exploring
denitrification genes from denitrifiers from culture collections and isolates from environmental
samples to extend the databases of functional genes by also considering the phylogeny of the
organisms.
References:
1. Hoffmann T, Horz HP, Conrad R, submitted.
2. Horz HP, Raghubanshi AS, Heyer J, Kammann C, Conrad R, Dunfield P, submitted.
3. Braker G, Fesefeldt A, Witzel K.P. 1998. Appl. Environ. Microbiol. 64:3769-3775.
4. Braker G, Tiedje, JM. 2001. Poster VAAM Jahrestagung 2001.
83
Project ID: 01LC0021 (Subproject 4)
1.1.2001 – 31.12.2003
BIODIVERSITY AND SUCCESSIONS IN TEMPORARY TERRESTRIAL-AQUATIC
INTERFACES
J. Pernthaler, A. Pernthaler, F. Warnecke and R. Amann
Max-Planck-Institut für Marine Mikrobiologie, Celsiusstrasse 1, D-28359 Bremen
jperntha@mpi-bremen.de
Key words: Actinobacteria, automated microscopy, substrate gradients
Abstract:
The diversity of microorganisms found in freshwater aquatic habitats is probably a small subset
of the total microbial biodiversity of terrestrial habitats. One aspect of the current project attemps
to establish the diversity and ecology of freshwater Actinobacteria. This group of gram positive
microbes is of considerable biotechnological interest, and many actinobacterial lineages of
terrestrial origin are known (5). Some Actinobacteria have recently been discovered to play a
prominent role in freshwater microbial communities, too (1), and moreover provide an interesting
model for food-web controlled microbial survival in habitats exposed to high and selective
mortality (4). Other facets of the project deal with the establishment of high-throughput methods
for the quantification of different microbial populations in water samples, and with laboratory
models that investigate the competitive performance of individual microbial populations exposed
to different substrate gradients (3).
Results:
During the first year, one focus of the project was the development and refinement of appropriate
methods for the culture-independent characterization of microbial diversity within one defined
phylogenetic group. A PCR-based protocol for the rapid screening of 16S rDNA clone libraries
from freshwater systems for comparatively rare actinobacterial sequences (typically 1-10% of all
obtained sequences) was developed. So far, 20 almost complete sequences from this lineage could
be obtained from several hundred screened clones, which more than doubles the available
database for freshwater Actinobacteria. After the successful establishment of the screening assay
we now progress to produce at least 50 different actinobacterial sequences from 4-5 aquatic
habitats that greatly differ in their temporal stability, and to search for eventual biogeographical
patterns. In addition, this collection will serve as a base for the design of specific oligonucleotide
probe for fluorescence in situ hybridization (FISH), so that the population sizes of different
actinobacterial lineages can be followed in selected habitats.
FISH probes are an increasingly popular tool to visualize different bacteria in various
environments. So far, the quantification of FISH-stained cells is tedious and time-consuming, and
the relative number of different bacteria in a sample have to be established by epifluorescence
microscopy and manual counting. This limits the application of the approach to relatively simple
ecological questions, and e.g., discourages more comprehensive studies of microbial
biogeographical distribution and population dynamics. We are, therefore developing techniques
for the automated counting of double-stained bacteria in environmental samples by computerassisted image analysis. In the first year of the project we have established an image acquisition
and evaluation system for the automated microscopic evaluation of comparatively simple
samples, i.e. the counting of bacterial cells in suspension that are filtered and immobilized on
membrane filters. For this we have developed a robust computerized focussing system that
84
combines a propriatory autofocus with Haralick texture parameters (2), feedback loops for image
contrast maximation, and decisions on image content during acquisition. The system is thus
capable of excluding inappropriate or badly focussed microscopic fields prior to evaluation
without operator interaction with high reliability, and to evaluate >50 FISH stained samples per
day at a total of 2000-3000 counted cells per sample. Future improvements are aimed at better
discrimination of bacteria and non-bacterial objects in complex (e.g., sediment) samples by means
of advanced shape recognition and/or colour.
A third line of research, although originally not explicitely focussed on freshwater
systems, is conceptionally nevertheless closely related to one of the basic ecological questions to
be adressed in the project. We tested the hypothesis that aquatic bacteria found in different niches
in the natural habitat (particle-attached and planktonic, respectively), might in principle also differ
in their adaptations to substrate gradients. Two bacterial isolates from a previously established
culture collection were exposed to different modes of substrate addition in batch cocultures.
During cocultivation on a batch of substrates the final cell densities of the free-living bacterial
strain were reduced three times as much as of the attached ecotype, as compared to growth yields
in pure cultures. In contrast, the gradual addition of substrates to stationary phase cocultures was
clearly disadvantageous for the population of the typically particle-attached bacteria (3). A
different growth response to substrate gradients could thus be another facet affecting the
competition between bacteria at the aquatic-terrestrial interface.
References:
1.
2.
3.
4.
5.
Glöckner, F.-O., E. Zaichikov, N. Belkova, L. Denissova, J. Pernthaler, A. Pernthaler, and R.
Amann, Comparative 16S rRNA analysis of lake bacterioplankton reveals globally distributed
phylogenetic clusters including an abundant group of Actinobacteria, Appl. Environ. Microbiol.,
2000, 66, 5053-5065.
Haralick, R. M., Shanmuga.K, and I. Dinstein, Textural features for image classification, IEEE
Trans. Syst. Man Cybern., 1973, 3, 610-621.
Pernthaler, A., J. Pernthaler, H. Eilers, and R. Amann, Growth patterns of two marine isolates:
Adaptations to substrate patchiness?, Appl. Environ. Microbiol., 2001, 67, 4077-4083.
Pernthaler, J., T. Posch, K. Simek, J. Vrba, A. Pernthaler, F. O. Glockner, U. Nübel, R. Psenner,
and R. Amann, Predator-specific enrichment of actinobacteria from a cosmopolitan freshwater
clade in mixed continuous culture, Appl. Environ. Microbiol., 2001, 67, 2145-2155.
Rheims, H., A. Felske, S. Seufert, and E. Stackebrandt, Molecular monitoring of an uncultured
group of the class Actinobacteria in two terrestrial environments, J. Microbiol. Methods, 1999, 36,
65-75.
85
Project ID: 01LC0021 (Subproject 5)
1.1.2000 - 31.12.2003
INVESTIGATION
OF
PROKARYOTIC
CULTIVATION METHODS
DIVERSITY
BY
OPTIMIZED
Frederic Gich, Herbert Hoffelner and Jörg Overmann
Bereich Genetik und Mikrobiologie, Department I, Fakultät für Biologie, LudwigMaximilians-Universität München, Maria-Ward-Str. 1a, D-80638 München
j.overmann@LRZ.uni-muenchen.de
Key words: Not-yet-cultured bacteria, TGGE, dialysis culture, molecular fingerprints,
cultivation, dialysis culture, chemotaxis
Abstract:
The biodiversity of selected groups of prokaryotes is investigated by a combination of
cultivation methods with culture-independent molecular techniques. The target groups are
chosen based on their numerical dominance in the natural environment. The present project
focuses on those prokaryotes which are not-yet-cultured, but have been shown to occur in
large populations in the environment, especially in soil. For a first inventory of the important
groups of prokaryotes, group-specific PCR protocols are established and combined with
temperature gradient gel electrophoresis (TGGE) to yield typical molecular fingerprints. A
suite of novel cultivation methods is employed for the enrichment, isolation and subcultivation
of prokaryotes. These methods comprise the formulation of novel mineral media, chemotactic
enrichments, growth on inert solid substrates, treatment with specific bacterial pheromones,
and dialysis cultures. Cultivation success is monitored by most probable numbers and by
molecular fingerprints to establish the most promising experimental strategies for the
isolation of certain dominant prokaryotes. By its combination of molecular biological
methods with cultivation techniques, the present project offers the perspectivetof ultimately
result in the isolation and characterization of ecologically significant prokaryotic isolates and
to understand their impact on the performance of the soil ecosystem. We expect that some of
the isolates obtained can also be employed as indicators for certain types of ecosystems.
Results:
Culture independent methods have repeatedly demonstrated that the diversity of prokaryotes in
the natural environment is unexpectedly high. This is especially true for soil, where the number of
bacterial species is estimated to range between 13,000 and 26,000 species [1,2]. By comparison,
only 4500 species of prokaryotes so far have been validly described [3]. The diversity in aquatic
habitats appears to be lower (~550 species, [4]). So far, only a minor fraction of the diverse and
ecologically significant assemblages of prokaryotes can be isolated in pure culture (i.e. 0.3% of
the total cell counts [5]) and is thus available for physiological studies. Traditional cultivation
techniques have been shown to select for bacteria which appear to occur in minor numbers only
[6]. The isolation and characterization of ecologically significant bacteria is thus a prerequisite for
an understanding of microbial diversity and the function of microbial assemblages in natural
environments.
In the present project eight taxonomic groups of prokaryotes were chosen based on their
occurrence in the natural environment: the Acidobacteria, Actinobacteria, Low GC gram-positive
bacteria, α-Proteobacteria, β-Proteobacteria, Cytophaga-Flexibacter-Bacteroides Group,
nonthermophilic Crenarchaeota, and the Green Nonsulfur Bacteria. Selective PCR protocols for
the amplification of 600 bp-long 16S rRNA gene fragments were established. In combination
with Temperature Gradient Gel Electrophoresis (TGGE), our technique permits a culture86
independent and rapid monitoring of the composition of natural communities. Since the DNA
bands generated are available for sequencing, the dominant prokaryotes of each group can also be
identified taxonomically in a cultivation-independent manner.
Fig. 1.
16S rDNA fingerprints of the group Green Nonsulfur Bacteria in various environments. Different numbers
correspond to different bacteria. All 16S rDNA sequences obtained were previously unknown (after [7]).
8
8
11
8
15
8
14
8
17
16
8
19
8
13
12
1
5
4
3
7
18
7
2
10
10
9
6
6
6
6
Several novel cultivation methods are employed for the enrichment, isolation and subcultivation
of prokaryotes especially from soil. Three different soils (fir forest, beech forest, alpine soil at
1400 altitude) located south of Munich were chosen as sampling sites. Our methods comprise the
formulation of novel mineral media, chemotactic enrichments, growth on inert solid substrates,
treatment with specific bacterial pheromones, and dialysis cultures. Cultivation success is
monitored by the most probable number (MPN). We divised a new MICRODROP technique for
the automated inoculation of defined numbers of single bacterial cells in liquid media in microtiter
plates. To date, a total of 200 bacterial strains could be isolated in liquid cultures with this
MICRODROP technique. Employing the group-specific fingerprinting methods described above,
the strains obtained are identified and compared to the original composition of the complex
prokaryotic community. By the combination of cultivation attempts with culture-independent
methods, the most promising experimental strategies for the isolation of certain dominant
prokaryotes will finally be established.
References:
1. Atlas RM, Horowitz A, Krichevsky M, Bej AK (1991) Microb Ecol 22, 249-256
2. Torsvik V, Sørheim R, Goksør J (1996) J Industrial Microbiol 17, 170-178
3. DSMZ,
Deutsche
Sammlung
von
Mikroorganismen
und
Zellkulturen
(2000)
http://www.dsmz.de/bactnom/bactname.htm
4. Jaspers E, Nauhaus K, Cypionka H, Overmann J (2001) FEMS Microbiol Ecol 36,153-164
5. Amann RI, Ludwig W, Schleifer K-H (1995) Microbiol Rev 59, 143-169
6. Dunbar J, Takala S, Barns SM, Davis JA, Kuske CR (1999) Appl Environ Microbiol 65, 1662-1669
7. Gich F, Garcia-Gil J, Overmann J (2001) Arch Microbiol (in press)
87
Project ID: 01LC0021 (Subproject 6)
1.1.2001 – 31.12.2001
THE ROLE OF N-ACYL-L-HOMOSERINE LACTONE (AHL) MEDIATED
COMMUNICATION FOR THE STRUCTURE AND PERSISTENCE OF
MICROBIAL RHIZOSPHERE COMMUNITIES
A. Steidle, K. Sigl, K. Riedel, and L. Eberl
Lehrstuhl für Mikrobiologie, Technische Universität München, Am Hochanger 4, 85350 Freising
EBERL@mikro.biologie.tu-muenchen.de; http//www.quorum-sensing.de
Key words: Quorum-sensing, rhizosphere, cell-cell communication, Pseudomonas putida,
Serratia sp.
Abstract:
The plant rhizosphere represents a highly complex ecosystem that is influenced by a number
of abiotic (soil chemistry, atmos- and hydrosphere, climate, etc.) and biotic factors (plant
species, number and diversity of rhizosphere organisms). The bacterial communities of the
rhizosphere have strong influence on the growth and health of the plant as well as on their
ability to adopt to changed environmental conditions. In recent years it has become evident
that a number of bacteria associated with plants produce N-acyl-L-homoserine lactone (AHL)
signal molecules that act as chemical communication signals between bacterial cells as well
as between bacteria and plants. These interactions appear to be of great importance for the
ecology of the rhizosphere. The aim of the proposed project is to investigate the role of AHLmediated intergeneric communication for the diversity and the stability/dynamic of the
rhizosphere ecosystem. These investigations should also allow to monitor changes of the
environment by assessing the fluctuations in the communication and population structure of
the rhizosphere consortium.
Results:
We have developed and characterized novel Gfp-based monitor strains that allow in situ
visualization of AHL-mediated communication between individual cells in the plant
rhizosphere. For this purpose three Gfp-based AHL sensor plasmids (1) were transferred into
AHL-negative derivatives of Pseudomonas putida IsoF and Serratia liquefaciens MG1, two
strains that are good colonizers of tomato roots. Each of these sensor plasmids is capable of
detecting a different range of AHL molecules, depending on the components used for its
construction. The resulting strains were rigorously characterized with respect to their
sensitivities for various AHL molecules and then employed to visualize AHL-based
communication between defined bacterial populations in the rhizosphere of axenically grown
tomato plants.
We screened over 300 bacterial strains isolated from the rhizosphere of tomato plants for their
ability to synthesize AHL signal molecules. Approximately 12% provoked a positive signal
with at least one of the AHL monitor strains used. We selected four isolates for further studies
and these were, on the basis of their 16S rDNA, affiliated to the genera Pseudomonas and
Rahnella. The AHL profiles (i.e. the amounts and structure of the AHL molecules) of these
isolates were determined by thin-layer chromatography in combination with the AHL
biosensor pSB403 (Fig. 1) (4, 2).
88
Fig. 1. Thin layer chromatographic analysis
of AHLs produced by bacterial strains
isolated from the tomato rhizosphere. AHLs
extracted from cell-free culture supernatants
of Pseudomonas putida IsoF (4), P. putida
Z2D (5), Rahnella aquatilis TAA (6), and R.
aquatilis T13 (7) were separated by TLC, and
spots were detected with the aid of the AHL
biosensor E. coli MT102(pSB403). Synthetic
AHLs were included as reference substances
as follows: 3-oxo-C6-HSL (1), 3-oxo-C12HSL (2), and 3-oxo-C10-HSL (3).
1
2
3
4
5
6
7
To investigate whether these strains do produce AHL molecules when colonizing the tomato
rhizosphere we inoculated axenically cultured tomato plants with an AHL monitor strain
together with an AHL-producing rhizosphere isolate. When the tomato roots were inspected
under a confocal laser scanning microscope, green fluorescent cells could be observed
indicating the presence of AHL molecules in the plant rhizosphere. Expectedly, no signals
were detected when the sensor strains were inoculated alone. In conclusion, these experiments
provide strong evidence that AHLs are produced by bacteria colonizing the rhizosphere of
axenically grown tomato plants and that these signals can be perceived by other bacteria (3).
Furthermore, we integrated an AHL sensor cassette, that responds to the presence of long
chain AHLs with the expression of Gfp, into the chromosome of the DsRed-tagged P. putida
strain F117. This monitor strain was used to demonstrate that the indigenous bacterial
community colonizing the roots of tomato plants growing in non-sterile soil produces AHL
molecules. In a first step this monitor strain was used to inoculate tomato plants grown in nonsterile soil together with the untagged wild type strain IsoF. As for the axenically grown
plants we observed that most red fluorescent cells were also green fluorescent indicating that
IsoF also produces AHL in the natural root habitat. However, in contrast to the gnotobiotic
system, we also detected green fluorescent cells when the monitor strain was inoculated alone.
These results clearly demonstrate that the indigenous rhizosphere community produces AHLs
at concentrations high enough to activate the monitor strain and thus provides strong evidence
that these signal molecules are utilized by the consortium for communication between
different populations.
References:
1. Andersen, J. B., A. Heydorn, M. Hentzer, L. Eberl, O. Geisenberger, B. B. Christensen, S. Molin, and
M. Givskov. 2001. Appl. Environ. Microbiol. 67:575-585.
2. Geisenberger, O., M. Givskov, K. Riedel, N. Høiby, B. Tümmler, and L. Eberl. 2000. FEMS
Microbiol. Lett. 184:273-278.
3. Steidle, A., K. Sigl, R. Schuhegger, A. Ihring, M. Schmid, S. Gantner, M. Stoffels, K. Riedel, M.
Givskov, A. Hartmann, C. Langebartels, and L. Eberl. 2001. Appl. Environ. Microbiol. 67. In press.
4. Winson, M. K., S. Swift, L. Fish, J. P. Throup, F. Jørgensen, S. R. Chhabra, B. W. Bycroft, P.
Williams, and G. S. A. B. Stewart. 1998. FEMS Microbiol. Lett. 163:185-192.
89
Project ID: 01LC0021 (Subproject 7)
1.1.2000-31.12.2003
BIODIVERSITY OF PROTOZOA IN THE RHIZOSPHERE OF PLANTS:
EFFECTS
ON
ACTIVITY,
PERFORMANCE
AND
DIVERSITY
OF
MICROORGANISMS AND ON PLANT GROWTH.
M. Bonkowski, K. Kreuzer and S. Scheu
Institut für Zoologie, Darmstadt University of Technology, Schnittspahnstrasse 3, D-64287
Darmstadt, Germany
bonkow@bio.tu-darmstadt.de; http://neuro.bio.tu-darmstadt.de/newww/zoology/scheu
Key words: manipulation of soil diversity, rhizosphere interactions, plant competition
Abstract:
Competition between plants is high, because plant growth in natural environments frequently
takes place in dense stands of established vegetation. Thus both, beneficial and deleterious
associations of plants with microorganisms and the regulation of these associations by soil
faunal activity may be of fundamental importance for individual plant species, but also for the
species composition of the whole community. We investigate the mechanisms and ecological
significance of interactions between plants and microorganisms (bacteria, fungi), and their
faunal regulation. We focus on three general aspects: (1) the regulation of plant growth by
microorganisms and their consumers (protozoa, nematodes, collembola), (2) the competition
for nutrients between microorganisms and plants, (3) the regulation of plant-plant
competition in the rhizosphere.
Results:
Hypotheses 1.3.1. Protozoa affect size, turnover, diversity and function of microbial
communities in the rhizosphere and 1.3.4. Protozoa increase the resilience of the microbial
community.
We isolated species of different protozoan taxonomic (amoebae, flagellates, ciliates) and
functional groups (bacterial, fungal feeders) from soil. An abundant soil amoebae
(Acanthamoeba sp.) was selected as model-organism and in cooperation with Prof. M.
Wagner, TU-München, TP2) could be genetically related to the A. castelanii group. We
transferred this naked amoebae with specific soil bacteria in monoxenic cultures. The effects
of amoebal grazing on the diversity of rhizobacteria and root growth of experimental plants
have to be tested in subsequent experiments.
A first protoype of a new microbial respirometer has been constructed in our department in
order to measure microbial activity, biomass, turnover and functional attributes on a
microscale.
Investigations of microbial diversity with molecular approaches are currently in progress.
Hypothesis 1.3.2. Protozoa increase plant growth by changing the microbial community in
the rhizosphere, by increasing nutrient cycling and hormonal effects.
In cooperation with Prof. I.T. Baldwin (MPI for Chemical Ecology, Jena) and Dr. B.S.
Griffiths (Scottish Crop Research Institute, U.K.) we are currently investigating the
interactions of protozoa and root-feeding nematodes in the rhizosphere of tobacco plants
(Nicotiana attenuata). Root herbivores such as nematodes are known as crop pests and may
strongly affect plant diversity in the field. Current studies, focussing on effects of visible
90
above-ground herbivory, demonstrated that tobacco plants can discriminate attacks from
different above-ground herbivore species. About 500 plant genes are involved in the
'recognition' and specific defense strategies against different herbivores. However, the
mechanism of plant response to below-ground herbivores is not yet known. In addition, the
interactions in the rhizosphere are complicated by species interactions. Protozoa are known to
increase root biomass due to hormonal effects from grazing on rhizosphere bacteria. Rootfeeding nematodes commonly have the opposite effect, but leaking of root cell contents due to
nematode feeding might fuel growth of rhizobacteria and stimulate subsequent effects of
protozoan grazing. We aim to get deeper insight in the molecular regulation of root growth
and plant defense to attack from root herbivores.
Hypothesis 1.3.3. Protozoa increase plant growth via interactions with mycorrhiza.
An experiment in cooperation with Prof. G. Bécard (C.N.R.S., Toulouse, France), testing
effects of protozoa and AM-fungi on growth of Plantago lanceolata is currently in
preparation.
Hypothesis 1.3.5. Protozoa affect the competition between plants via species specific effects
on microbial communities and root morphology.
In cooperation with Dr. J. Roy (C.N.R.S., Montpellier, France) we investigated the
influence of microbial diversity on growth and competition among grasses in experimental
microcosms.
The performance of the six different grass species was highly dependent on soil diversity
(F = 13.0, p = 0.0001 for the interaction plant species x soil diversity). A subsequent
regression of the average shoot biomass per plant species in a sequence of increasing soil
diversity (sterile < microorganisms < microfauna < mesofauna) was taken as a measure of
plant performance in relation to soil diversity.
The homogeneity of slopes (ANCOVA), gave significant differences for three out of the
six investigated plant species. A significant negative slope (F = 4.4, p = 0.04) for Bromus
hordeaceus indicated a decrease in shoot growth with increasing soil diversity in
monocultures. Bromus species turned out to be weak competitors in mixtures compared to
monocultures. Particularly B. hordeaceus gained the lowest shoot biomass in competition
with other plant species in the mixture treatment. However, with increasing soil diversity
shoot biomass of B. hordeaceus increased in plant mixtures (F = 4.8, p = 0.04). This was due
to a release of Bromus from competition with Aegilops geniculata (F = 5.3, p = 0.03) and B.
madritensis (F = 15.8, p < 0.001) which both were negatively affected by increasing
biodiversity. The two strongest competitors in plant mixtures, Hordeum murinum and Lolium
rigidum did not respond to any soil diversity treatment.
In conclusion, soil biodiversity had strong effects on the performance of certain grasses
when different plant species were in competition. Apparently, slight modifications in the
fitness of a grass by soil organisms multiplied to strong effects when the plant had to compete
with other grass species. Since competition among plants is the natural situation in the field,
our experiment indicates that effects of soil biodiversity on grassland community structure
have so far been underestimated.
Hypothesis 1.3.6. Protozoan effects on plant growth depend on the diversity of the faunal
community in the rhizosphere.
This hypothesis is tested by including other faunal groups in experiments with soil protozoa
(see examples in hypotheses 1.3.2. and 1.3.5.). Our preliminary results indicate that species
interactions in the soil food-web are of major importance for understanding faunal effects on
rhizosphere microorganisms and plant growth.
91
Project ID: 01LC0101 (Subproject 1)
01.04.2001-30.09.2003
AN ANALYSIS OF PREVAILING ECONOMIC AND POLITICAL STRATEGIES
FOR THE PROTECTION OF BIODIVERSITY - THE TRADE IN GENETIC
RESSOURCES: A VIABLE CONTRIBUTION TO THE PROTECTION OF
BIODIVERSITY?
S.L. Franck-Oberaspach1 , O. Deke2 , G. Klepper2 * und P.T. Stoll1 ,3 **
1
Max-Planck-Institute (MPI) for Foreign Public Law and International Law, D-69120 Heidelberg,
Im Neuenheimer Feld 535, e-mail: sfranck@mpiv-hd.mpi.de;
2
Institute for World Economics (IfW), D-24100 Kiel, Düsternbrooker Weg 120; e-mail:
gklepper@ifw.uni-kiel.de, odeke@ifw.uni-kiel.de;
3
New adress: Institut für Völkerrecht der Universität Göttingen, D-37073, Platz der Göttinger
Sieben 5, e-mail: pstoll@gwdg.de; *Project leader IfW Kiel; **Project leader MPI Heidelberg
Keywords: biological diversity; genetic resources; public good; property rights; access and
benefit sharing, value, trade
Abstract:
It is the purpose of this sub-project to assess the potential to contribute to the conservation of
biodiversity of the international trade in genetic resources (GR). Initial results show that the
contribution of the trade in genetic resources to the conservation of biological diversity
probably will be limited even without taking into account the concerns unsually cited here,
e.g. the incomplete implementation of relevant international legislation or the possibly
inefficient use of monetary returns by providers of genetic resources.
Results:
Genetic resources are often considered as one of the major contributions of biodiversity
(Swanson 1996). In addition, it is often assumed that genetic resources are the major
privatizeable good in the vector of outputs produced by biodiversity. We define, for the
purpose of our study, as genetic resources (GR) only biological material the genetic
information of which is directly used by a potential recipient. The biological material itself
(e.g. wood or a medical plant that is used as raw material, i.e. a pharmaceutical is directly
extracted) is not regarded as a genetic resource here since it has different economic properties:
biological material is a rival, exclusive good. Genetic information, to the contrary, is a public
good as soon as the biological material it is embedded in is publicly accessible. Exclusive
possession of a biological material implies the exclusive possession of the genetic information
contained. Access to a genetic resource in this case can be provided via sale of the material, or
via a contract that entitles the providing party to a percentage of the income derived from this
genetic information. Accordingly, legal experts discussed as a major problem the
implementation of legislation to regulate access and benefit sharing (e.g. Glowka 1997).
Economists, on the other hand, tried to assess supply of and demand for genetic resources,
including the problem of of transboundary habitats, that may cause ‚races to the bottom‘ of
market prices of genetic resources1 . Recent economic research also focuses on the survey of
bilateral contracts in order to examine their design and economic efficiency (e.g. Rausser and
Small 2000).
1
A related problem is the fact that biodiversity- rich areas amy be substituted, in part, by ex situ collections in a
partial consideration, i.e. if only the production/conservation of genetic resources is looked at.
92
We follow a more specific, theoretical approach in the first part of our analysis. We consider
appropriabilities in the GR-related industries and markets, i.e. in all phases of GR-related
research and development (R&D) processes and marketing of respective R&D results. We
examine different institutional frameworks (legal rights of exclusion) and their potential
effects on property rights as defined by Barzel (1989). Initial results gained in the area of
plant genetic resources for food and agriculture (PGRFA) show that weak property rights
have a major impact on the market value of genetic resources. These property rights, however,
do not simply depend on an institutional framework (i.e. legal rights of exclusion) that can be
created and implemented at the discretion of the national or international lawmaker, since the
physical properties of the materials and R&D processes involved largely determine the
possibilities of effective legislation, mainly by influencing the enforcability of contracts
and/or intellectual property rights. Inherent public good aspects, however, are likely to be
much smaller in other industries.
Further study will focus on the assessment of the gap between the social and private value of
genetic resources, and on the potential of (international) legislation and implementation of
existing legislation to close this gap.
References:
Barzel, Y. (1989): Economic analysis of property rights. Cambridge University Press.
Glowka, L. (1997): Emerging legislative approaches to implement Article 15 of the Convention on
Biological Diversity. Review of European Community & International Environmental Law 6
(3): 249ff.
Rausser, G.C. And Small, A.A. (2000): Valuing research leads: bioprospecting and the conservation
of genetic resources. Journal of Political Economy 108 (1), 173-206.
Swanson, T. (1996): The reliance of northern economies on southern biodiversity: biodiversity as
information. Ecological Economics 17, 1-8.
93
Project ID: 01LC0101 (Subproject 2)
01.04.2001-30.09.2003
AN ANALYSIS OF PREVAILING ECONOMIC AND POLITICAL STRATEGIES
FOR THE PROTECTION OF BIODIVERSITY - THE PROTECTION OF
BIODIVERSITY: NEW APPROACHES AND INSTRUMENTS IN INTERNATIONAL
LAW
S.L. Franck-Oberaspach1 , O. Deke2 , G. Klepper2* und P.T. Stoll1,3**
1
Max-Planck-Institute (MPI) for Foreign Public Law and International Law, D-69120
Heidelberg, Im Neuenheimer Feld 535, e-mail: sfranck@mpiv-hd.mpi.de;
2
Institute for World Economics (IfW), D-24100 Kiel, Düsternbrooker Weg 120; e-mail:
gklepper@ifw.uni-kiel.de, odeke@ifw.uni-kiel.de;
3
New adress: Institut für Völkerrecht der Universität Göttingen, D-37073, Platz der Göttinger
Sieben 5, e-mail: pstoll@gwdg.de; *Project leader IfW Kiel; **Project leader MPI Heidelberg
Keywords: biological diversity; international regimes; international law, economic
incentives, transfer mechanisms; institutional coordination
Abstract:
The protection of biodiversity is a relatively new objective for the international community. In
1995, the prevailing international treaty in the field, the Convention on Biological Diversity
(CBD), entered into force. Its efficient implementation, however, has yet to be achieved.
Another unresolved task is the harmonization of this new objective with related goals (e.g.,
the protection of species, landscapes, or cultural achievements of indigenous peoples) and the
effective adaptation of the existing system of international law towards this objective. The task
includes, inter alia, innovative mechanisms with respect to financial transfers. Her, the
existing international regime aiming at the protection of biodiversity is analysed. Economic
analysis focuses on incentives provided by available instruments. Legal analysis focuses on
the identification and examination of existing treaties and regulations that often do not
mention ‚biodiversity‘ but focus on elements thereof. The systematic connection of these
international treaties is analysed. Our final goal for this first part of the BIOLOG project is
directed towards the description of possibilities of efficient implementation and institutional
coordination at the international level.
Results:
The term ‚biodiversity‘ requires and promotes the joining together of regimes and instruments
that have originally been intended to promote the protection either of ‚parts‘ of biodiversity
(e.g., single species) or of areas (e.g., certain landscapes) with existing regimes especially
designed to protect biodiversity (maily the Convention on Biological Diversity (CBD, entered
into force in 1995), with bodies of the United Nations in charge of environmental problems
(e.g., the United Nations Environmental Programme (UNEP) and the Commission on
Sustainable Development (CSD)), and with to-be-constructed new instruments.
In the actual phase of the project, we concentrate on an analysis of existing international
regimes and instruments. We identified a considerable amount of international regimes and
instruments the objective of which is the protection of biodiversity-related elements. The
origin of most of these treaties is pre-CBD; they do not usually refer to the ‚biodiversity‘
directly. Most of these regimes do not have mechanisms for international financial transfer.
Reseach with respect to the legal aspects of these instruments has largely focused on problems
related to the collision of norms between different regimes (e.g. Raustiala, 1997). Aspects of
94
institutional coordination, however, only rarly have been examined in-depth. Economic
research, on the other hand, has analysed incentives provided by these regimes, and incentives
of differents regimes have been compared to each other (e.g. Moyle 1998). Results of these
economic approaches led to the proposal of potentially more effective new instruments.
Aspects of compatibility of these instruments with the existing system of international
regimes, however, have been largely neglected by most of these approaches.
We identified as one major goal, at the theoretical/analytical level as well as with respect to
the actual implementation of biodiversity-related regimes, the systematic joining together of
existing treaties, instruments, and regulations that have potential to contribute to the
protection of biodiversity-rich areas with available mechanisms of financial transfer(s).
Another major objective, again at the theoretical/analytical level and with respect to the actual
implementation, is the analysis and development of possibilities to impose sanctions on
countries that do not comply with the objective of the conservation of biodiversity. Both
aspects involve very complex problems already at the level of international law and
governance. Our preliminary conclusion is that there are serious problems to be solved at the
international level, and that the constant degradation and the progressive loss of biodiversity
is not a problem of implementation at the national level alone.
References:
Rosell, M. (1997): The sovereignty challenge. In: Review of European Community & International
Environmental Law, 6 (3), 274-283.
Moyle, B. (1998): Species Conservation and the principal agent problem. Ecological Economics 26:
313-320.
95
96
BIOLOG AFRICA
97
Project ID: 01LC0024 (BIOTA Southern Africa)
01.10.2000 – 30.09.2003
A RESEARCH PROJECT MONITORING AND ANALYSING BIODIVERSITY AND
ITS CHANGE ALONG A TRANSECT IN NAMIBIA AND SOUTH AFRICA
BIOTA Southern Africa is located in Namibia and the Republic of South Africa. BIOTA
Southern Africa has an integrated interdisciplinary research approach on biodiversity in
Africa that includes the following disciplines: animal production, botany, climatology,
modelling, mycology, remote sensing, socio-economics, soil science, and zoology.
The project is active along an approximately 2000 km long transect that extends from the
Mediterranean Fynbos Biome at the Cape, the semi-arid Mediterranean Succulent Karoo
Biome, the arid Desert Biome, the semi-arid tropical Nama Karoo Biome up to the tropical
semi-arid Savanna Biome. Based on this transect approach, the target area of BIOTA
Southern Africa covers the entire area parallel to the main rainfall gradient, from the
temperate Cape with its winter rainfall climate up to the semi-humid Kavango region of
north-eastern Namibia.
Within the three BIOTA AFRICA regional projects, BIOTA Southern Africa examines the
most arid regions. Hereby, the project activities are located at either margin of the arid
subtropical high pressure cell (wider Karoo-Namib region).
Scientific goals of BIOTA Southern Africa
The need to develop new strategies to deal with the dramatic environmental changes in Africa is a
fundamental challenge to the scientific community. The protection and management of
biodiversity plays a key role in such strategies. The overall goal of BIOTA Southern Africa is to
gain knowledge for decision-makers for a feasible and sustainable management of biodiversity.
Within the framework of long-term interdisciplinary fieldwork, applicable land use systems are
analysed in combination with scientifically based conservation concepts for the resource
biodiversity. Socio-economic information is fundamental for the comprehension and the
management of the systems.
In order to understand the bio-physical and socio-economic drivers affecting biodiversity, some
relevant questions are:
•
•
•
•
•
•
•
To what extent are environmental factors responsible for biodiversity?
How important are abiotic and biotic soil compartments and mechanisms?
How important are slow processes and preceding environmental and evolutionary history?
What are the mechanisms by which land use practices control biodiversity and vice versa?
How important are spatial movements of taxa and environmental factors?
Are future changes of biodiversity predictable?
What is the social, economic, and political importance of biodiversity and how can it be
valued?
In contrast to dense rainforests, relatively open savanna, shrubland, and semi-desert
vegetation allow a more convenient long-term monitoring. In these semi-arid vegetation
types, BIOTA Southern Africa has established a standardised monitoring approach that
considers the following aspects:
•
•
Based on remote sensing and GIS, a large-scale approach monitors changes to vegetation
structures, to soil characteristics, and to biodiversity within a specified transect band.
More than 30 Biodiversity Observatories have been established along the transect band.
These Observatories represent a large number of major ecosystems and vegetation units.
98
•
Often, these Biodiversity Observatories are arranged in pairs, in identical ecosystems, but
exposed to different land use practices. This allows the identification of management
practices and land use intensities and their impact on natural resources.
Biodiversity Observatories ensure the comparability of research sites within the BIOTA
Southern Africa project. The standardisation of scales and applied methods, as well as the
integration of different disciplines in a defined research area are the major advantages of this
research approach.
Priorities of the first months
Directly after the start in October 2000, and after the formation of the working groups,
BIOTA Southern Africa principally focused on the following important issues:
•
•
•
•
Strengthening links with the partners and research institutions in Namibia and the
Republic of South Africa (MoUs, contracts)
Strengthening links with landowners and local communities (MoUs, contracts)
Preparation and carrying out of a first Kick-Off-Trip with all project heads
Organisation / establishment of the project´s infrastructure (field equipment, cars, weather
stations, logistic and research stations, surveyors, permits)
Practical steps of the first months
As a result of these activities, all practical preparations for the field work had been completed
by April 2001, and can be summarised as follows:
•
•
•
•
•
Several workshops were organised by our Head office in Hamburg/Germany. Three
workshops took place during the Kick-Off-Trip in March and April 2001 in the host
countries (two in Cape Town, one in Windhoek). The detailed and multidisciplinary
presentation of BIOTA Southern Africa´s intentions and methodology were discussed
with the scientific community of Namibia and the Republic of South Africa. The project
initiative was welcomed, and in some cases an even stronger integration into the national
scientific communities was requested. Based on the motivating spirit of co-operation,
clear agreements on the future co-operation were defined. The formation of the “South
Africa BIOTA Liaison Committee” (SABLC) will guarantee transparent discussions and
support well-founded decisions on the future and joint development of the BIOTA
AFRICA project in South Africa.
A number of more technical matters had to be settled: six cars, technical field equipment,
and weather stations were bought. The issues of registration, insurance, and safe storage
were solved.
30 Biodiversity Observatories and a very large number of additional sampling sites have
already been established in co-operation with national and local institutions and
landowners. For an easy orientation in the field, the Observatories have been subdivided
into 100 hectares, and numbered. Weather stations were installed and have started with
data recording. For all sites, monitoring protocols and general rules were defined, under
which field work in the Biodiversity Observatories is to be carried out.
For a large proportion of Observatories, first inventories of organisms have been
documented. This baseline information can be used by all scientists who work in
accordance with the scientific needs as defined by the Namibian and South African
scientific community and responsible institutions.
At several conferences, preliminary field results have been presented to a broad scientific
community, and discussion papers are in the making.
99
Project ID: 01LC0024 (BIOTA Southern Africa S01)
01.10.2000 – 30.09.2003
REMOTE SENSING AND GIS BASED SURVEY OF SPATIAL AND TEMPORAL
BIODIVERSITY DYNAMICS AND ANALYSIS OF BIODIVERSITY AND
GEODIVERSITY INTERRELATIONSHIPS
B. Hörsch, M. Vogel, C. Schultz and G. Braun
Deutsches Zentrum für Luft- und Raumfahrt DLR, DFD-UG, Linder Höhe, 51147 Köln-Porz
bianca.hoersch@dlr.de - http://www.dfd.dlr.de
Key words: Remote Sensing, GIS, Biodiversity-Geodiversity, Monitoring, Vegetation
Mapping, Capacity Building
Abstract:
This project shall create the basis for a system in which both, remote sensing and GIS
applications, form an analysis and monitoring system for the assessment of human impacts on
biological diversity. It is our aim to make the existent biodiversity more understandable and
predictable, taking into account the UN-Convention on Biological Diversity (UNCBD).
It is planned to establish a coherent GIS along the entire BIOTA transect in order to allow the
analysis of spatial patterns of biodiversity, its temporal changes and the mutual dependency
of geodiversity and biological diversity. Focus will lie on the comparison of natural
ecosystems with those where socio-economic activity has led to various degradation levels of
the former natural ecosystems. Furthermore, the detection of indications of spatial patterns of
diversity by remote sensing techniques will contribute to future monitoring and conservation
concepts.
Further tasks are the “capacity building” for co-operating partners within the fields of
Remote Sensing and GIS.
Results:
Within the last 14 months the data base of a Remote Sensing based GIS for the monitoring of
present-state biodiversity within the Southern African transect was built up (1). In close
interaction with the project partners the different localities of field work were identified during a
field trip in March and respective observatories were geographically referenced.
Tasks performed included first of all the investigation of appropriate multitemporal data sets from
commercial multispectral remote sensing systems (LANDSAT-7-ETM, IKONOS, CORONA
etc.). A set of LANDSAT-7-ETM scenes was acquired, covering the identified biodiversity
observatories along the transect. All satellite scenes ordered were georectified to the common
UTM system and were integrated as image base into the GIS. Additionally, aerial photogrphs of
the Namibian observatories were georectified and added to the data base.
Besides Digital Chart of the World (DCW)-data basic GIS data (topography, infrastructure) were
integrated into the spatial database at a scale of 1: 50.000. For Namibia, the integration of farm
boundaries, settlements, river and road networks has been completed, whereas Southern African
data based on topographic maps at 1: 50.000 are partly still being processed. These primary
informations were further used to produce geometrically referenced image maps which are
essential as an auxiliary means for the orientation of the fieldworkers. They also act as a base for
mapping training samples which form the basis in the supervised classification of vegetation
patterns from the multispectral images (2, 3). The maps were enhanced by topological
information (roads, settlements, farm boundaries) and were superimposed by a fine resolution
latitude/longitude grid in order to facilitate orientation in the field and to enable the direct
comparison of GPS recordings with mapped features. The maps were distributed to the project
partners in paper printed form. On request, digital maps were also supplied.
100
An important feature of a spatial database supporting biodiversity analysis in a more indirect way
are topographical data (4, 5). They form not only the basis for the absolute geographical
orientation of features in space, but are essential to derive topoclimatological information like
potential water and solar irradiance distributions in the terrain (6, 7). Due to the lack of fine scale
topographical maps, interferometric analysis of European Remote Sensing Satellite (ERS)-tandem
data has been performed to derive Digital Terrain Models from the research areas. Respecive data
for Namibia have almost entirely been processed. For Southern Africa, topographic information to
produce Digital Terrain Models is being purchased at the moment, as appropriate ERS-data are
lacking. Due to the importance of spatial climatic data for biodiversity modelling, research had
been carried out on downscaling spatially coarse climatic datasets in order to derive climatic
information on a spatial scale which is compatible to the scale of the research approach (8, 9).. Up
to now, long term (1960-1990) precipitation and temperature data have been downscaled to a
spatial resolution of 5km and 2 km, respectively, as long-term monthly means. In the near future it
is intended to add additional climatic parameters and to cover the area with non-aggregated
monthly mean data.
Resulting from various field campaigns by the project partners of subprojects S05 and S06
vegetation training samples have been integrated into the spatial database for study areas at the
Richtersveld/Fish River Canyon, the Knersvlakte and the Wlotzka´s Baken area. Based on these
samples vegetation maps have been derived from respective LANDSAT-7-ETM scenes.
Validation of these maps is in progress.
To support the use of spatial data and information systems by the project partners two capacity
building activities took place at the DLR in Cologne and at the Institute of Botany in Hamburg.
Topics of the first course were primarily remote sensing basics with a special emphasis on sensor
sensitivity, reflectance of different surfaces, and generation and interpretation of false colour
composites. The second course held in Cologne focussed on Remote Sensing basics in
combination with GIS architecture for biodiversity research and monitoring (11). Topics like
general data structure, the integration of referenced geospatial together with Remote Sensing data
and the main steps for building up of an information system were dealt with. Integration
possibilities of raster and vector based spatial data with ecological/biological attributes stored in
databases were addressed partially.
References:
1.
2.
3.
4.
5.
6.
7.
8.
9.
Dale, V.H., Offerman, H. et al. (1995): Landscape characterization and biodiversity research. In:
Boyle, T.J.B. & Boontawee, B.: Measuring and Monitoring Biodiversity in Tropical and
Temperate Forests; Proceedings of a IUFRO Symposium held at Chiang Mai, Thailand August
27. - September 2. 1994, Centre for International Forestry Research: Bogor, Indonesia.
Allner, T., Backhaus, R. et al. (1992): Biodiversity Indication by Earth Observation Data. In:
Proc. Of the Central Symp. of the Int. Space Year Conference, Munich, Germany. 30 March- 4
April 1992, 863-867.
Podolsky, R. H. (1990): Monitoring Biodiversity and Landscape Richness with digital Earth
Imagery. In: Archives of Photogrammetry and Remote Sensing Vol. 28, Part 7-1, Proceedings of
the Symp. On Global and Encironmetal Monitoring.
Burnett, M. R., August, P. V. et al. (1998): The influence of Geomorphological Heterogeneity on
Biodiversity. A Patch-Scale Perspective. In: Conservation Biology, 12, 363-370.
Nichols, W. F., Killingbeck, K. T. et al. (1998): The Influence of Geomorphological
Heterogeneity on Biodiversity. A Landscape Perspective. In: Conservation Biology, 12, 371-377.
Antonic, O., Marki, A. et al. (2000): A Global Model for Monthly Mean Hourly Direct Solar
Radiation. In: Ecological Modelling, 129, 113-118.
Florinsky, I. V., Kulagina, T. B. et al. (1994): Influence of Topography on Landscape Radiation
Temperature Distribution. In: Int. Journal of Remote Sensing, 15, 3147-3153.
Franklin, J. (1998): Predicting the Distribution of Shrub Species in Southern California from
Climate and Terrain-derived Variables. Journal of Vegetation Science, 9, 733-748.
Aspina ll, R. (1992). Spatial analysis of wildlife distribution and habitat in a GIS. In: Proc. 5th
International Symposium on Spatial Data Handling.
101
Project ID: 01LC0024 (BIOTA Southern Africa S02)
01.10.2000 – 30.09.2003
EDAPHICAL DIVERSITY AND BIODIVERSITY IN MUTUAL DEPENDENCY
A. Gröngröft, A. Petersen and G. Miehlich
Institut für Bodenkunde, Universität Hamburg, Allende-Platz 2, 20146 Hamburg
G.Miehlich@ifb.uni-hamburg.de
Key words: soil variability, pH value, Namibia
Abstract:
Soil as one major compartment of terrestrial ecosystems has a significant impact on the
composition of terrestrial flora and fauna and vice versa. The soil science BIOTA project
aims to analyse the mutual dependency of the soil as a controlling factor of biodiversity along
the entire BIOTA transect following a continental climatic gradient of high geodiversity. The
documentation of the soil diversity, especially with respect to the interdependency of soil,
flora and fauna, requires a standardised and applicable methodology which proofs to be
powerful also in the analysis and interpretation of land use practises and management
strategies. This includes the selection of useful indicators to document the influence of soil
parameters on the biodiversity.
General Aims:
The main focus of the Soil Science BIOTA project lies on the inter-disciplinary approach to
analyse the structures, reasons, processes, and impacts of biodiversity along a gradientorientated transect. The hypothesis that a higher variability in abiotic parameters in a given
area will necessarily lead to a higher biodiversity is to be proofed. Considering that abiotic
parameters are of varying importance to flora and fauna depending on the biotic group to be
analysed, this project does not only focus on a standardised documentation of soil diversity,
but also considers the problem of scale. Different methodological scales have to be applied
when analysing (a) changes in soil parameters according to changes in vegetation units and/or
analysing the effects that single bushes or trees have (b) on animals (shading effect) or (c) on
plants (safe sites for germination). Important questions to be answered include whether or not
a systematic nutrient or salt accumulation takes place, which effects small burrowing
mammals create on soil properties and wether soil crusts by cyanobacterias or lichens have an
impact on the nutrient cycle of the soil and thus again on the biotic diversity. Furthermore,
the impact of different land use practises on the geodiversity will be analysed. As one major
abiotic part of terrestrial ecosystems the soil and thus this project forms an important
explanatory and functional link between the various biotic aspects (fauna, vegetation, lichens,
crusts, fungi) within the BIOTA project.
Results:
In this first phase main focus lies on the testing of soil spatial variability in the Biota
observatories. For the selection of the sampling sites within the observatory a stratified
random procedure was developed, in which the observatories are stratified in different habitat
types, according to topography, vegetation patterns etc.. Each hectare square is assigned to a
habitat type.
102
During the first field work phase in 2001 seven observatories along the Namibian part of the
BIOTA transect were investigated. A total of 134 soil profiles each with a depth of 0,6 to
1,5 m were described by field parameters, documented by photos and sampled for laboratory
analysis. The following table gives an overview on the analysed observatories and the number
of soil profiles.
Tab. 1: Sampled Biota observatories with number of soil profiles
Observatory
01 Mile 46 Research St.
02 Mutompo
03 Sonop Research Station
04 Toggekry 250
05 Otjiamongombe West
10 Gellap Ost
11 Nabaos
R = by ranking
Where appropriate, the standardised
sampling routine following the
ranking procedure was completed by
a local transect approach, e.g. when
linear dune systems dominate/
characterise
an
Observatory
(Observatory No 2: Mutompo).
Number of soil profiles
6 (T)
20 (R u. T)
12 (T)
40 (R)
26 (R)
15 (R)
15 (R)
T = Transect
First laboratory results of pH values (in H2 0) in the topsoil of all observatories show distinct
differences in mean values as well as variability along the transect (Fig. 1) from North
Namibia with higher rainfall and more sandy soils to South Namibia with lower precipitation.
Within one observatory the different habitat types (by topography, vegetation etc.) are
characterised by varying pH values of the topsoils, as shown by Fig. 2 for the example of
observatory Toggekry. The habitat types with the higher plant biodiversity (Habitat 1 and 2)
are associated with topsoil of elevated mean pH-values and larger pH variability than the
other habitat types with lower plant diversity and more sandy soils.
7
9
8
6
7
6
5
5
4
Fig. 1 pH in topsoils: all observatories
Habitat5
Habitat4
Habitat3
Habitat2
Habitat1
Nabaos
Gellap Res.
Otjiamongombe
Toggekry
Sonop
Mile 46 Res.
Mutompo
4
Fig. 2 pH in topsoils: habitats of Toggekry
103
Project ID: 01LC0024 (BIOTA Southern Africa S03)
01.10.2000 – 30.09.2003
BIODIVERSITY OF SOUTHWESTERN AFRICAN FUNGI: INTERACTIONS WITH
HIGHER PLANTS, FUNCTIONAL DIVERSITY AND DNA-TAXONOMY
R. Berndt1 , C. Görke 1 , E. Langer2 , M. Mennicken1 , E. Uhlmann1 and F. Oberwinkler1
1
Spezielle Botanik/Mykologie, Botanisches Institut, University
of Tübingen, Auf der
Morgenstelle 1, D-72076 Tübingen;
2
Vertretungsprofessur für Ökologie, FB 19 Biologie/Chemie, University of Kassel, HeinrichPlett-Str. 40, D-34123 Kassel
claudia.goerke@uni-tuebingen.de - http://www.systbot.uni-tuebingen.de
Key words: Arbuscular mycorrhizae, Namibia, rust fungi, soil fungi
Abstract:
Our project aims to ascertain data on the biodiversity and ecology of soil fungi, arbuscular
mycorrhizae, and plant parasitic rust fungi in important biomes of southwestern Africa.
During a first trip to Namibia in March 2001, samples were taken on the observatories Mile
46, Sovo, Sonop 903, Otjiamongombe, Toggekry 250, Gellap Ost 3 and Nabaos 7. First
observations and results from this trip are presented here.
Results:
Soil samples examined for the presence of arbuscular mycorrhizal spores revealed differences
in the number of spores and – although species identification is still pending – species
composition. The dominating spore type in the north (Mile 46 and Sovo) is a small, dark
brown Glomus-spore, in the thornbush savanna (Otjiamongombe, Toggekry 250) a light
brown Glomus-spore and in the south (Gellap Ost 3 and Nabaos 7) a small, yellowish-brown
Glomus-spore. The dominance of Glomus-spores is in accordance with the reports by Stutz et
al. (1). The number of spores is within the range reported in previous studies from comparable
ecosystems (2, 3).
Root samples stained for the presence of arbuscular mycorrhizal fungi were mainly found to
be mycorrhizal with the exception of Poaceae in Nabaos 7. As spores were present in soil
samples near the plants from which the non-mycorrhizal roots were taken, this warrants
further attention. Molecular studies (especially on the samples from Nabaos 7) are under way
to check for the presence of non-staining mycorrhizal fungi and to verify the results obtained
by microscopic techniques.
The soil samples that were examined for arbuscular mycorrhiza were used for the study of soil
fungi as well. Following the procedure of Gams & Domsch (4) the samples were washed with
6 l of water and transferred to isolation media. Over thousand cultures of soil fungi were
isolated. A part of the species is already identified, but identification of most isolates is still
pending. So far, the isolates that have been identified belong to 20 genera; eight genera seem
to be new to Namibia.
The preliminary results confirm the impression gained during the study of arbuscular
mycorrhiza, that Gellap Ost 3 and Nabaos 7 are different from the other areas. From the soil
in Gellap Ost 3 and Nabaos 7, fewer fungi were isolated. The number of Zygomycetes is
especially low, possibly due to the lack of organic litter. The dominating isolate was a
Coelomycete. However, in this area samples were taken before the onset of the raining
season, while the raining season in the other areas was well under way. Therefore, further
sampling is necessary to compare the areas under the same water conditions.
104
Our work on rust fungi in Namibia aims to contribute to the rust mycota of selected
biodiversity observatories and the whole country.
The knowledge about Namibian rust fungi is extremely scanty and mostly derived from the
works of Ethel Doidge in southern Africa (e.g. 5). Although the Namibian flora comprises ca.
4.000 species of higher plants (6) our extensive literature search revealed only 13 species of
rust fungi (comp. Tab. 1). This species number is extremely low compared with figures
available for other African countries [e.g. South Africa ca. 400 (7), Uganda 294 species (8)].
A first species inventory of the BIOTA observatories in 2001 indicates that a considerably
higher number of rusts can be expected to occur in Namibia.
Tab. 1: Rust species hitherto reported for Namibia (compiled from literature)
Rust fungi
Host/Family
Host/Species
Aecidium acanthopsidis H. & P. Sydow
Acanthaceae
Acanthopsis sp. Harv.
Aecidium clarum Sydow
Iridaceae
Ferraria viscaria Schinz
Aecidium dinteri Doidge
Mimosaceae
Acacia uncinata Engl.
Aecidium lebeckiae P. Hennings
Fabaceae
Lebeckia simsiani E. & Z.
Aecidium talinophilum P. & H. Sydow
Portulacaceae
Talinum caffrum (Thunb.) Eckl. & Zeyh.
Puccinia desertorum Sydow
Convolvulaceae
Evolvulus alsinoides (L.) L.
Puccinia chaseana Arth. & Fromme
Poaceae
Anthephora schinzii Hack.
Puccinia galeniae Dietel
Aizoaceae
Galenia africana L.
Galenia sarcophylla Fenzl.
Puccinia sorghi Schwein.
Syn.: Puccinia maydis Bereng.
Syn.: Aecidium oxalidis Thuem.
Poaceae
Zea mays
Oxalidaceae
Oxalis obtusa Jacq.
Uredo augeae H. & P. Sydow
Zygophyllaceae
Augea capensis Thunb.
Uromyces comptus H. & P. Sydow
Convolvulaceae
Merremia bipinnatipartita (Engl.) Hallier f.
Uromyces saginatus Sydow
Hyacinthaceae
Drimia altissima (L.f) Ker Gawl.
Uromyces trollipi Kalchbr. & MacOwan
Zygophyllaceae
Zygophyllum sp. L.
References:
1. Stutz, J. C., Copeman, R., Martin, C. A., Morton, J. B., Can. J. Bot., 2000, 78, 237-245: Patterns of
species composition and distribution of arbuscular mycorrhizal fungi in arid regions of
southwestern North America and Namibia, Africa
2. Gaur, A., van Greuning, J. V., Sinclair, R. C., Eicker, A., South African Journal of Botany, 1999,
65, 434-436: Arbuscular mycorrhizas of Vangueria infausta Burch. subsp. infausta (Rubiaceae)
from South Africa
3. Michel-Rosales, A., Valdés, M., Mycorrhiza, 1996, 6, 105-109: Arbuscular mycorrhizal
colonization of lime in different agroecosystems of the dry tropics
4. Gams, W. & Domsch, K. H., Archiv für Mikrobiologie, 1967, 58, 134-144: Beiträge zur
Anwendung der Bodenwaschtechnik für die Isolierung von Bodenpilzen
5. Doidge, E. M., Bothalia, 1950, 5, 1-1094: The South African fungi and lichens to the end of 1945
6. Craven, P. (ed.), Southern African Botanical Diversity Network Report, 1999, 7, 1-206: A checklist
of Namibian plants
7. Crous, P. W., Phillips, A. J. L., Baxter, A. P., Phytopathogenic fungi from South Africa, 2000, 1358
8. Kåre, A. L., Namaganda, M., Gjærum, H. B., Lidia, 2000, 5, 9-12: Ugandan rust fungi 1. Key to
genera
105
Project ID: 01LC0024 (BIOTA Southern Africa S04)
01.10.2000 – 30.09.2003
DELVELOPMENT OF AN INTEGRATED SYSTEM FOR THE INPUT, STORAGE
AND ANALYSIS OF BIODIVERSITY DATA IN SOUTHERN AFRICA
A: G. Rambold1 , R. Siber2 , B. Weber2 ; B: B. Marbach1 , R. Siber2 , B. Weber2 and C: B.
Büdel2 , G. Rambold1
1
2
Universität Bayreuth, Lehrstuhl für Pflanzensystematik, Abt. Mykologie, Universitätsstrasse 30
– NW I, 95440 Bayreuth
Universität Kaiserslautern, Fachbereich Biologie, Abt. Allgem. Botanik, Postfach 3049,
67653 Kaiserslautern
gerhard.rambold@uni-bayreuth.de
http://www.uni-bayreuth.de/departments/planta2/wgr/fmycology.html
Key words: biological soil crusts, checklists, database, ecological and descriptive data, GIS, GUI,
interoperability, functional diversity, LIAS, modelling, Mycology.Net, spatial analysis
Abstract:
A central task of BIOTA subproject S04 was the development of a uniform standardized
method for data collection in the field. According to the observatory transect method, all data
collected in the field were categorized into four different hierarchical levels. The development
of the database module “DiversityEcology”, which reflects this hierarchical structure,
represents the second major achievement of the project. A third major task is the development
of a graphic user interface (“DiversityNavigator“) for remote editing and analysis of data
being stored in Postgres implementations of database modules, based on the “Diversity” data
models, which have been developed within the BIOLOG-project GLOPP.
Results:
Three main issues had to be addressed during the first year of the project:
A) Composition of a common method for the data collection in the field
B) Development of a software structure for the recording, storage and analysis of project data
C) Establishment of a Java-based application („DiversityNavigator“) for the request and
processing of data from the Diversity Database Modules via internet
A) Composition of a common method for the data collection in the field
One main task of the project is the development of a uniform standardized method for data
collection in the field to enable interdisciplinary analysis and modeling. According to the
observatory transect method, which forms a common standard of the three BIOTA groups, all
data collected in the field could be categorized into four different hierarchical levels. The selection
and classification of the parameters, which are collected on the different levels, was realized in
accordance to the Database-project SOTER (Global and National Soils and Terrain Digital
Databases) of the FAO (FAO, 1995). The uppermost level contains data on the observatories as
units, like their geographic coordinates, the vegetation type and the local climate. One level
below, data on the habitats delimited against each other within the observatories is combined. On
the third level, all data on the sites which are investigated by the researchers are combined. The
detailed characterization of the investigated sites is a fundamental requirement for spatial analysis
and modeling of the distribution patterns of species. On the undermost level, data on all
specimens which are investigated or collected in the field and on the applied sampling method are
stored. In concordance with all subprojects of BIOTA South the parameters being collected on the
106
two upper levels of the database were determined and their classification adopted to the local
conditions. The parameters of the two lower levels were elaborated together with subproject S05.
Only such a standardized interdisciplinary approach facilitates detailed spatial and statistical
analyses and the yield of new knowledge.
B) Development of a software structure for the recording, storage and analysis of project data
The implementation of a modern networked software architecture with continuous database
access and fully developed GIS analysis functionality was another major task of the first project
period. The database SQLServer was chosen as DBMS, since this software also allows to process
geographic coordinates. A direct database connect of the GIS-software was realized via an
ArcSDE gateway. This software architecture facilitates the storage of all data within a
geodatabase, which allows an object oriented data structure. ArcInfo was selected as GISsoftware, because of the comprehensive analysis and visualization techniques and the new
functionalities in the area of geostatistical analysis. For the analysis of remote sensing and other
raster data Erdas Imagine is applied. In the near future, the software ArcIMS will be applied to
present the results via internet in the form of interactively generated maps.
During the last months, the database module “DiversityEcology” was created, in which
the data collected in the field by BIOTA South (data on the observatories and habitats) and all
data collected by subproject S05 are stored. The database module was implemented under
consideration of the Relational Model by Codd (CODD, 1990) and the normalization rules. The
database “DiversityEcology” is an independent database module, that has an interface to the other
existing Diversity Database modules. The data input in the database module is realized by a
graphical user interface (GUI), that facilitates the input and editing of the acquired data via
internet.
C) Establishment of a Java-based application („DiversityNavigator“) for the request and
processing of data from the Diversity Database Modules via internet
The database modules being developed in the BIOLOG-projects GLOPP (01LC9905) and now
being provided to public on the Internet (www.glopp.net) offer suitable tools for the
maintenance of taxonomic data (DiversityTaxonomy), literature (DiversityReferences),
descriptive data (DiversityDescription) and collection data (DiversityCollection). Their implementation under MS Access will be completed in the near future. The establishment of the
Java-based graphic user interface “DiversityNavigator”, established in Biota S04, will allow
an Internet-based, platform-independent usage of these databases. The first period of this
project was dedicated to the establishment of various program technical basics like the
definition of data exchange formats, the establishment of data import routines and an experimental prototype network, as well as the establishment of Diversity-compatible Postgres
databases. A recently completed draft version of the DiversityNavigator enables the user to
import and edit the taxonomic and collection data, stored in Postgres databases. It furthermore allows parallel data queries of databases, which may be located under several domains.
The interoperability of the modules could be successfully tested by a configuration with the
two database modules DiversityTaxonomy and DiversityReferences which enable users to
maintain and query species checklist data. In the forthcoming period of the project the
established technique will be used to store and maintain all taxonomic and descriptive data
gathered in subproject S05.
References:
1. Codd, 1990: The Relational Model for Database Management, Version 2. Menlo Park.
2. Fao, 1995: Global and National Soils and Terrain Digital Databases (SOTER). World Soil
Resources Reports 74, Rev. 1, 1-125.
107
Project ID: 01LC0024 (BIOTA Southern Africa S05)
01.10.2000 – 30.09.2003
BIOLOGICAL SOIL CRUSTS OF TERRESTRIAL ECOSYSTEMS: MODES OF
PATTERN FORMATION AND THEIR MODELLING CONCERNING DIVERSITY,
STRUCTURE AND FUNCTION IN CRYPTOGAMIC COMMUNITIES
A: B. Büdel1 , K. Loris 3 , V. Wirth8 , L. Zedda 6 – B: S. Dojani1 , T. Feuerer2 , M. Küppers 3 ,
R. Lücking 4 , S. Ott5 , W. Reisser7 , M. Salisch7 , V. Wirth8 – C: B. Büdel1 , G. Rambold6
1
Universität Kaiserslautern, Fachbereich Biologie, Allgemeine Botanik, Postfach 3049, D67653 Kaiserslautern
2
Universität Hamburg, Botanisches Institut und Botanischer Garten, Ohnhorststraße 18, D22609 Hamburg
3
Universität Hohenheim, Botanisches Institut, Biologie II, Garbenstraße 30, D-70599 Stuttgart
4
The Field Museum, 1400 S. Lake Shore Dr., Chicago, Il 60605-2496, U.S.A.
5
Universität Düsseldorf, Botanisches Institut, Universitätsstraße 1, D-40225 Düsseldorf
6
Universität Bayreuth, Lehrstuhl für Pflanzensystematik, Universitätsstraße 30-NW 1, D95447 Bayreuth
7
Universität Leipzig, Botanisches Institut, Talstraße 33, D-04103 Leipzig
8
Staatliches Museum für Naturkunde, Erbprinzenstraße 13, D-76133 Karlsruhe
Contact person: buedel@rhrk.uni-kl.de, http://www.uni-kl.de/FB-Biologie/AG-Buedel/
Key words: biological soil crusts, biodiversity, lichens, algae, cyanobacteria, vegetation
ecology, microclimate, dew fall, fog
Abstract
An overview of the field and laboratory work performed so far is given and first results are
presented. In almost all observatories along the transect, biological soil crusts, composed of
either cyanobacteria, algae or lichens, or a combination of them, have been discovered.
Biomass, expressed as milligrams chlorophyll a per m², ranged from 1 to 80 mg/m². So far, 14
genera of cyanobacteria, 21 genera of green algae and 32 lichen species have been
determined. A new species of the lichens genus Peltula was discovered growing underneath
translucent quartz pebbles with an inverse internal thallus morphology.
Special care was given to the establishment of measuring devices for fog and dew fall along a
40 km long gradient from the coast to the inland at Wlotzkasbaken, Namib Desert, in order to
correlate this data with the vegetation pattern of lichens.
Results
It is the intention of our project to study the species (cyanobacteria, algae and lichens) diversity, pattern formation and biomass of biological soils crusts and their role in the ecosystem.
We want to find out their dynamics, their establishment after disturbance and their influence
on the diversity of the phanerogamous vegetation (e.g. N input via cyanobacterial N-fixation).
Three of us took part in the kick-off trip and actively participated in the selection of
the observatories. A key on the priority of interest was developed and assigned to the newly
established observatories. High priority was granted to observatories with well-developed
biological crusts and high lichen diversity (12 observatories). Intermediate priority was
assigned to observatories without biological soil crusts, but with a rich epiphytic and epilithic
lichen flora (8 observatories), where only biodiversity studies are carried out. Observatories
without biological crusts or lichens will not be investigated by S05 at this stage.
During this trip, first collections were made and a very rough estimation of the
presence or absence of soil crusts of each observatory was achieved. At each investigation
site, 4 samples were collected for the following purposes: determination of a) cyanobacterial
108
diversity, b) green algal diversity, c) N- and C-content as well as the isotope fractionation, d)
chlorophyll a/b content. Two main classes of crusts can be distinguished 1) pioneer crusts
(succession stage after disturbance) and 2) climax crusts (part of permanent vegetation).
Further differentiation can be made according to the dominating taxonomical group (e.g.
cyanobacterial, algal, lichen or moss crust). Determinations of biomass, expressed as
chlorophyll a content per m², resulted in values between 1 and 80 mg/m², with an average of
33 mg/m² for the first 16 samples along the transect. However, this can only be seen as very
preliminary results. This is also the case for the first diversity analysis of cyanobacteria and
algae. In total, we found cyanobacterial species (free living and in culture) belonging to 14
different genera and green algal species (in culture only), belonging to 21 different genera.
In the Nama Karoo, a new species of the lichen genus Peltula was discovered (fig. 1),
growing underneath quartz pebbles, exposing its cyanobiont towards the quartz and thus
having an inverse internal morphology. The new description of this species is in preparation
and will be published in 2002.
An additional transect from the coast 40 km to the inland was established in the lichen
field of Wlotzkasbaken, meeting the observatory in the center. Along this transect, measuring
devices for fog and dew fall were installed in order to gather data on the events and amount.
Those results will be correlated with the very peculiar vegetation pattern of lichens in this
area. The biodiversity of the lichen vegetation along this transect was investigated on 21 stations and to a distance of 35 km from the coast. Close to the coast, lichen coverage rises distinctly for a short distance, then diminishes constantly inland along the transect. Most species
occur from the coast to the end of the investigated transect section, frequency of some species
however changes dramatically. At least one species occurs only at inland stations, several
occur only near the coast.
Lichens were collected in the observatories 18 (Koeroegap), 20 (Numees), 21 (Groot
Derm 10), 22 (Quaggafontein 478), 26 (Faminkvlakte 111), 27 (Luiperskop 211), 28 (Moedverloren 208) and 29 (Rocherpan) in order to investigate the general biodiversity. In the
observatory of Numees vegetation relevés on soil were also carried out by means of a new
method. It consists of taking digital pictures of cryptogamic communities on a soil surface of
60 x 60 cm using an aluminum square frame construction. Since May 2001, lichens have been
identified in the laboratory. TLC was applied to many samples. Problematic specimens of
various genera were sent to specialists. A first list of lichens forming biological soil crusts
includes 32 species. Information on lichen characters are entered into a Diversity database
module, and those on lichen specimens in the collection database module (http://www.diversitycam-pus.net). For the analysis of digital pictures, the program PanoramaFactory
(freeware) is used to compose the entire relevé of 60 x 60 cm from 36 single digital pictures.
109
Project ID: 01LC0024 (BIOTA Southern Africa S06)
01.10.2000 – 30.09.2003
CHANGES IN BOTANICAL BIODIVERSITY WITH REGARD TO CHANGES IN
LAND USE PRACTICES AND CLIMATE: STANDARDISED MONITORING AND
TRANSECT ANALYSIS
N. Jürgens , B. Strohbach; M. Akhtar-Schuster, R. Austermühle, T. Becker, B. Hachfeld,
U. Schmiedel, M. Strohbach
Coordinators:
Prof. Dr. Norbert Jürgens, Botanical Institute, University of Hamburg, Ohnhorststr. 18, D-22609
Hamburg, Germany; e-mail: juergens@botanik.uni-hamburg.de
Ben Strohbach, National Botanical Research Institute, Private Bag 13184, Windhoek, Namibia, email: bens@mweb.com.na
Key words: Phytodiversity, vegetation mapping, community ecology, population ecology,
population dynamics, monitoring, land use impacts, desertification
Abstract: Vegetation plays an important and multidirectional role within the mechanisms
that control biodiversity. Firstly, vegetation forms an important resource for humankind and
is strongly influenced by land use practices. Secondly, vegetation is controlled by
environmental factors and, therefore, is an indispensable indicator for documenting
ecological changes. Thirdly, due to its structural, biological, and ecological properties,
vegetation itself is an important driver of biodiversity. The botanical subproject S06 aims at
long-term analyses and monitoring of the vegetation in over 30 Biodiversity Observatories.
Results:
Earth's biodiversity includes “all organisms and species, their immense genetic variation, as
well as their complex assemblages of communities and ecosystems...“ DIVERSITAS
PROGRAM Based on this broad definition, S06 integrates the assessment and analyses of
structures and mechanisms at different temporal and spatial scales. The development of a
general working protocol will supply all surveyors with a standardised methodology for all
Biodiversity Observatories.
Structural diversity: The mapping of vegetation along the BIOTA transect band, from the
Kavango to the Cape, will allow large and medium scale analyses of the vegetation structure.
This can only be achieved by the application of remote sensing techniques (S01). The
vegetation map is in the validation process. It will serve for an in-depth understanding of the
impact of changing natural gradients, e.g. rainfall gradient, as well as the influence of
different land use systems [1,2,3].
Community diversity:
The species composition of plant communities reflects environmental conditions. Based on a
multi-scale standardised working protocol, a large number of vegetation data were sampled
and classified into different vegetation units. Within the 1 km² large BIOTA Observatories,
the assessment of the spatial patterns of different communities was carried out on a regular
grid of 100 hectare.
Species diversity:
In 2001, for a large proportion of BIOTA Observatories, first inventories of flora and
vegetation were documented at four different spatial scales, ranging from 100m², 1,000m²,
10,000m² up to 1 square kilometre (Table 1). In some cases, these are linked to demographic
analyses of populations and species, focusing on migratory dynamics. Additionally, the
monitoring of the BIOTA Observatories also considers the individual and population level
(e.g. location, size, number of fruits) [4,5,6]. The long-term monitoring of the permanent plots
in the western Richtersveld (South Africa) forms the basis for the simulation models in
subproject S10.
110
Tab. 1: Surveyed Biodiversity Observatories in 2001
Observatory
Mile 46 & Mutombo
Sonop 903
Otjiamongombe West 44 &
Omathako Ranch
Ovitoto 55
Wlotzkasbaken, Mile 14
Nico 377 & Reserve
Gellap Ost 3 & Naboas 7
Karies 8, Gondwana Canyon
Park
Koeroegap Vlakte
Numees
Groot Derm 10
Quaggafontein 478
Leliesfontein 624
Remhoogte 416
Faminvlakte 111
Luiperskop 211
Moedverloren 208
Rocherpan Nature Reserve
District, Country
Rundu, Namibia
Grootfontein, Namibia
Okahandja, Namibia
Surveyor
B. Strohbach
B. Strohbach
R. Austermühle
Okahandja, Namibia
Swakopmund, Namibia
Mariental, Namibia
Keetmanshoop, Namibia
Karasburg, Namibia
B. Strohbach, T. Sheujuange
B. Hachfeld
M. Akhtar-Schuster
M. Akhtar-Schuster
N. Jürgens
Namaqualand, South Africa
Namaqualand, South Africa
Namaqualand, South Africa
Namaqualand, South Africa
Namaqualand, South Africa
Namaqualand, South Africa
Van Rhynsdorp, South Africa
Van Rhynsdorp, South Africa
Vredendal District, South Africa
Piquetberg, South Africa
C. Mayer, M. Müller, N. Jürgens
N. Jürgens
M. Müller, C. Mayer, N. Jürgens
U. Schmiedel
U. Schmiedel
U. Schmiedel
U. Schmiedel
U. Schmiedel
U. Schmiedel
S. Milton & Co-workers
Intraspecific diversity:
Intraspecific diversity has been established on the level of ITS 1+2 and will be carried out by
using AFLP. Samples were taken from the Succulent Karoo Biome (Aspazoma, Cheiridopsis)
in August 2001.
Functional diversity:
In 2001, we started with the sampling of diaspore inventories at all BIOTA Observatories in
order to understand the dynamics and regeneration capacity of the species. This will be
continued. In the Succulent Karoo, recording of ecophysiological adaptations started with
regard to salinity stress, ion composition, and water relations at the observatories Numees and
Yellow Dune in the Richtersveld, South Africa.
Further activities: The S06 team is composed of researchers who already have long-term
field experience in southern Africa. Due to this, a major part of the organisation and
implementation of the project, i.e. the kick-off-trip, workshops, preparation of MoU´s,
logistics, and infrastructure was carried out by our team.
References and selected publications:
[1] Strohbach, B.J., 2000, Dinteria 26, 77-92: Vegetation degradation trends in the northern Oshikoto
region: III The Terminalia prunioides woodlands and Andoni grasslands.
[2] Strohbach, B.J., 2000, Dinteria 26, 93-112: Vegetation degradation trends in the northern Oshikoto
region: IV. The Broad-leafed savannas with associated pans.
[3] Hachfeld, B., Jürgens, N. 2000, Phytocoenologia 30(3-4), 567-589, Climate patterns and their
impact on the vegetation in a fog driven desert: The Central Namib Desert in Namibia.
[4] Jürgens, N., Gotzmann, I.H. and Cowling, R.M., Plant Ecol., 1999, 142, 87-96.: Remarkable
medium-term dynamics of leaf succulent Mesembryanthemaceae shrubs in the winter-rainfall desert
of northwestern Namaqualand, South Africa.
[5] Hachfeld, B., 2000. Dinteria 26, 113-146: Rain, fog, and species richness in the Central Namib
Desert in the exceptional rainy season of 1999/2000.
[6] Schmiedel, U., Jürgens, N., 1999, Plant Ecology 142, 57-69: Community structure on unusual
habitat islands: quartz-fields in the Succulent Karoo, South Africa.
111
Project ID: 01LC0024 (BIOTA Southern Africa S07)
01.10.2000-30.09.2003
FUNCTIONAL ZOODIVERSITY IN SOUTHERN AFRICA UNDER CHANGING
ENVIRONMENTS AND HUMAN USE
U. Zeller1 , M. Ade 1 , J. Deckert 1 , S. Eiseb2 , P. Giere 1 , A. Hoffmann1 , F. Koch1 , E.
Marais 2 , W. Mey1 , J. Plötner1 , M. Uhlig1 , K. Vohland1 , H. Wendt 1
1) Museum für Naturkunde, Humboldt Universität, Invalidenstr. 43; 10099 Berlin, Germany;
contact person email: ulrich.zeller@rz.hu-berlin.de
2) National Museum of Namibia, Robert Mugabe Avenue 59; Windhoek, Namibia
Key words: Functional zoodiversity, food webs, reproduction, population ecology
Abstract:
The main objective of this study is to learn about biodiversity, its composition (taxonomy),
how it is functioning and how it can be maintained and managed as seen from a zoologists
perspective. In the pilot phase research is focused on small ground living mammals, their reproduction and their interactions with arthropods and plants, especially trophic interrelationships between these groups.
Hypotheses:
We hypothesize that
1) specific small mammal communities differ in their direct influence on vegetation and soil
characteristics by their digging habits seed dissemination and bioturbation in general;
2) specific small mammal communities directly influence arthropod species richness and
abundance mainly due to predation, and thus indirectly affect vegetation communities (consumption of herbivores, pollinators, detritivores...);
3) the differential resource utilisation due to changing nutritional demands of the small mammals in the course of reproduction contributes significantly to vegetation and arthropod community composition;
4) large mammals (in different land use practices) and climate (esp. water availability) influence performance of soil, vegetation, arthropods, and small mammals. Accordingly the functional relationships and ecosystem performance differ between differently managed farms and
climatic zones;
5) the above mentioned indicators of biodiversity can be used for the formulation of management tools with respect to global change.
Results:
Investigations took place on iodiversity observatories in Karies 8 (Fish River Canyon), Gellap
Ost - Nabaos (Berseba) and Otjiamongombe (Erichsfelde) - Toggekry (Omatako) in Namibia,
April - Mai 2001. In South Afrika the biodiversity observatories Yellow dunes, Numees and
the Koerogapvlakte at the Richtersveld were sampled in October 2001.
One main goal of the first phase of the project is to develop and optimize adequate methods to
long term monitor biodiversity. Plots within the biodiversity observatories were selected according to the ranking method developed by BIOTA-South. A first species inventory was begun. Arthropods were collected with Malaise traps, different light traps, pitfall traps, yellow
plates, sweeping nets, pyrethrum fogging and various methods of hand collecting.
A first analysis of the well-known bio-indicator group Carabidae (ground beetles), based on
pitfall trap and light trap sampling as well as singling, showed the following results:
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Whereas the differences in
species inventory, diversity and
abundance of the ground beetles
communities between Karies and
Gellap Ost are obviously due to
meteorological, geological, geobotanical, zoogeographical and
geographical factors on the
BIOTA transect, the great
difference between the Karakul
sheep research farm Gellap Ost
and the over-grazed observatory
Nabaos results from the influence
of different land use.
Trapping for small mammals consisted of a 150 m transect
holding 30 Sherman traps and three bucket traps with some
additional traps selectively placed in bushes and trees. The
composition of trapped species reflected the assumption
according to literature research; except for Mus minutoides,
for which the most nothern record could be supplied for the
Koerogapvlakte in the Richtersveld.
Comparable to the arthropods, small mammals differ in their
species composition and abundance according to the position
on the BIOTA transect and the intensitiy of land use. Again
Nabaos is impoverished in biodiversity, only the widely distributed G. vallinus was trapped.
To analyse trophic relationships, the stomach content of selected small mammals was investigated. For this, the stomach was removed from the dead animal and emptied completely. The
volume was measured and the content was first analysed according to volumen relations. A
detailled analysis on a more elaborated taxonomic basis followed.
Anatomy of the reproductive tract and embryology of most trapped small mammal species
was only poorly or not at all known. First results show that the anatomical structure and reproductive status of the animals deviated from other well documented animals. These data
provide a necessary basis for the newly developed method of ultrasonic investigation for the
in-vivo determination of the reproductive status in the field.
Discussion and outlook
Monitoring zoological biodiversity is a compromise between effort and results. We can either
cover the transect of observatories completely, focus on selected biodiversity observatories to
obtain a more comprehensive data set, or record annual cycles.
The last point will be approached for the adjacent biodiversity observatories in Gellap Ost and
Nabaos, as here studies on population dynamics on small mammals have started to gather information on population processes and time slots for reproduction. For the main phase, more
emphasis will be put on population processes, and functional hypothesis approximated by first
models will be tested in the field.
Another point concerns sustainability, which can only be achieved if the project is accepted
both in Namibia and South Africa. For this, we are working on the implementation of a MSccourse in "Functional Biodiversity" in cooperation with the University of Namibia. Furthermore, native students are introduced into our project and employed in.
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Project ID: 01LC0024 (BIOTA Southern Africa S08)
01.10.2000 – 30.09.2003
DIVERSITY OF NAMIBIAN DRAGONFLIES: EFFECTS OF ANTHROPOGENIC
CHANGES AND MODELLING ON DIFFERENT SCALES
Frank Suhling 2 , Erik Braune 1 , Heike Hadrys 3 , Andreas Martens 2 , Dagmar Söndgerath1
and Otto Richter1
1
Institut für Geographie und Geoökologie, Technische Universität Braunschweig, Langer
Kamp 19c, D-38106 Braunschweig, o.richter@tu-bs.de, d.soendgerath@tu-bs.de,
e.braune@tu-bs.de.
2
Zoologisches Institut, Technische Universität Braunschweig, Fasanenstraße 3, D-38106
Braunschweig, f.suhling@tu-bs.de, andreas.martens@tu-bs.de.
3
Institut für Tierökologie, Tierärztliche Hochschule Hannover, Bünteweg 17, D-30559
Hannover, h.hadrys@ecol.evol.de.
Key words: Odonata, ephemeral rivers, species and community diversity, population
diversity, isolation, habitat suitability, population development, distribution maps.
Description of the project and first results
The aim of our project is the development and application of a system for the modelling of
Odonata diversity — on population, species and community level — in arid and semiarid
regions of Africa. The model-system will help to understand the dynamics of Odonata
communities and to evaluate different scenarios of global change and anthropogenic effects
for ecosystem-management. Odonate diversity can be used as an indicator in this context.
Faunistic survey, monitoring and experimental studies
The Odonata fauna of Namibia consists of 100 species (Martens et al. in prep.). The highest
species diversity – reflecting the presence of permanent rivers – is in the NE (Jödicke &
Martens in prep.). To study diversity in arid regions on the species and community level we
survey the Odonata of about 250 aquatic habitats in the western catchments, namely the
Swakop, Kuiseb, Tschauchab, Ugab, Huab and Hoanib, and, additionally, in the Fish river.
We recorded 52 species in these systems. We characterized the environment and the
geographical situation of all sites. Based on these data habitat suitability models (see below)
will help to evaluate the relevance of factors like habitat duration, vegetation, salinity, altitude
and connectivity for the structuring of Odonata assemblages and the distribution of species. A
number of sites are selected for long term monitoring to study phenology and variations in
assemblage structures. Using artificial pond systems we studied habitat selection, life cycles,
phenology, and priority. Our studies revealed clear patterns of habitat choice via oviposition,
which effects larval density in different structured ponds (Suhling et al. 2001). All species of
these ponds were able to develop within less than two months; Pantala flavescens requires
only 31 days from oviposition to emergence. The short development allows species to use
even short existing habitats and may be a major trait of desert Odonata (Suhling et al. in
press). The field studies are accompanied by laboratory experiments, which revealed clear
effects of priority, growth rate and intraguild predation in a two species assemblage,
combining the widespread species Trithemis kirbyi and Sympetrum fonscolombii.
Molecular genetics
The molecular genetic analyses of several ncDNA and mtDNA marker reveal first data on the
genetic diversity and genealogical relationships of the selected study species. The data
provide first insights into spatial and temporal dynamics of selected key-species in correlation
to different ecological settings (see below). The latter will help us to identify pathways used
114
by Odonata to colonize the ephemeral desert habitats, which is not possible with traditional
methods. The study includes different geographical scales: (a) between habitats in one
catchment, (b) between catchments, (c) between the western catchments and other regions of
Namibia (such as the savanna regions in the northeast), and (d) between Namibia and other
regions like Kenya, in co-operation with BIOTA E07. Based on monitoring data we selected a
set of key species, e.g. Paragomphus genei, which exclusively colonizes habitats within the
floodplains. Others such as Crocothemis erythraea and Anax imperator are not limited, since
they are excellent flyers. We compare the population structures/dynamics of these species in
connection with differences in their mobility. Using additional information on environmental
stress related genes (e.g. variation in heat shock proteins) provide insights into the adaptive
potential of the selected species. Our study also includes genetic information on rare and
extremely isolated species, which is a major task when setting up a conservation management
plan. For analyses at the genus level we have chosen Pseudagrion, Orthetrum and Trithemis,
which seem to be promising model systems to obtain insights into different stages of
speciation and differences in species diversity patterns in combination with recent and
historical habitat shifts.
Ecological modelling
Modelling is done on different scales. On the habitat scale suitability and population dynamic
models will be developed and applied for selected species. We use logistic regression
methods to model habitat suitability. These models are based on presence / absence data
together with habitat parameters recorded during the survey (see above). As a result we get
probabilities of occurrence in a given habitat for each species. Up to now data of the first
survey were explored to find species suitable for modelling and to run first suitability models.
For population dynamics we used a model based on “delay-differential-equation”, evolved by
Crowley et al (1987) for Odonata of the temperate region. This model was fitted to the
specific conditions of tropical desert Odonata using literature data as well as data of our own
studies (Schroeder 2001). However, since we found that this model will cause some problems
while combining it with habitat suitability models we decided to use the Leslie-matrix model
instead, which allows the incorporation of more complex data. On the catchment scale
different habitat models are coupled by a geographical information system (GIS) to form a
multi-habitat model. To do so we take into account the spatial spread of the individuals and
the gene flow between different populations. With this model system we will describe the
diversity of Odonata under different scenarios, e.g increase of drought, increase of water use,
building of dams. We produce distribution maps (basing on GIS), which allows the
application of countrywide models the diversity in the second phase.
Identification keys
As a general preparation we produced keys to the adults and larvae of the Namibian Odonata,
which will be published with the National Museum of Namibia, Windhoek. The larval key
already allows identification of all genera, but not all species, since a number of larvae are
undescribed. The adult key already allows identification of most of the species.
References
Crowley, P.H., R.M. Nisbet, W.S.C. Gurney & J.H. Lawton 1987. Population regulation in animals with
complex life-histories: formulation of a damselfly model. Adv. Ecol. Res. 17: 1-59.
Schroeder, E. 2001. Analysis of population dynamic models for dragonflies based on delay-differential
eqautions. Diploma-thesis, Technische Universität Braunschweig, 89+XIV pp.
Jödicke, R. & A. Martens 2002. The Odonata of Caprivi. Int. J. Odonatol. in press.
Martens, A., R. Jödicke & F. Suhling 2002. Odonata of Namibia. a priliminary checklist. Cimbebasia
Suhling, F., R. Jödicke & W. Schneider 2002. The Odonata of African arid regions: are there desert species?
Proceedings of the 1st Symposium on African Odonata. Cimbebasia
Suhling, F., K. Schenk & T. Padeffke (2001): Dragons of desert ponds: Colonization patterns, life cycles and
structural diversity. International Symposium of Odonatology, Schweden.
115
Project ID: 01LC0024 (BIOTA Southern Africa S09)
01.10.2000 – 30.09.2003
STRUCTURAL AND SPECIES DIVERSITY IN SEMI-ARID SAVANNAHS OF
SOUTHERN AFRICA: THE IMPACT OF LAND USE AND CLIMATIC CHANGE
F. Jeltsch, M. Schwager and J. Tews
Coordinator: Prof. Dr. Florian Jeltsch, Plant Ecology and Nature Conservation, Institute for
Biochemistry and Biology, University of Potsdam, Maulbeerallee 2, D-14469 Potsdam, Germany;
e-mail: jeltsch@rz.uni-potsdam.de
German Partners: Prof. Dr. P. Poschlod (Nature Conservation, University of
Marburg/Regensburg); Prof. Dr. A. Rasa (Zoology, University of Bonn); Dr. K.-H. Müller
(Geography, University of Marburg); Prof. Dr. R. Brandl (Ecology, University of Marburg/ UFZ
Halle); PD Dr. M. Bollig (Ethnology, University of Cologne)
South African Partners: Prof. Dr. S.J. Milton (Nature Conservation, University of Stellenbosch);
Dr. W.R.J. Dean (FitzPatrick Institute for Ornithology, University of Capetown); Prof. Dr. N. van
Rooyen (Botany, University of Pretoria)
Key words: population ecology, extinction risk, spatial-explicit simulation models, remote
sensing, landscape structures
Abstract:
In arid and semiarid savannahs species diversity is strongly influenced by the structural
diversity (landscape structures at different scales), which in these systems is characteristically
formed by woody vegetation. The southern Kalahari based subproject S09 uses an
interdisciplinary approach to investigate these structural-dependent relationships, combining
field studies (zoological, botanical, genetic and socio-economic), remote sensing, and spatialexplicit simulation models. The aim of the project is to evaluate the impact of current and
realistic future land use intensities and climatic change scenarios on the survival of selected
animal and plant species and on species diversity in general.
Results:
According to a basic definition of ‘biodiversity’ (UN Environmental Program 1992)
biological diversity means the variability among living organisms and the ecological
complexes of which they are part. This not only includes species diversity but also the
diversity of ecological structures or ‘structural diversity’. Structural diversity encompasses
both functional structures such as food webs and spatial structures, i.e. landscape structures at
different scales. Both elements of biodiversity – species and structural diversity – are closely
interlinked. Any changes in habitat structure inherit the risk of species loss. The aim of this
interdisciplinary project is an improved understanding of the complexity of impacts of
landscape dynamics (structural diversity) on species diversity under the major driving forces
in the southern Kalahari, namely land use and climatic change.
Structural diversity: - In the case of the southern Kalahari structural diversity is
mainly formed by woody vegetation. Major impacts on structural diversity in this system can
be addressed to land use and climate change. Land use, e.g. in the form of cattle farming or
fire wood cutting have evolved major changes of landscape structure. Socio-economic
surveys show, among other results, that the majority of farmers are utilizing camelthorn trees,
both for domestic and commercial purposes. Roughly about 20 % of the farmers
commercially utilize the wood and remove very large quantities. Long- to mid term changes
of tree cover ratios have also been quantified by remote sensing techniques using satellite
images and aerial photographs covering the last 60 years. An automatic tree detection
116
software has been developed writing a vector database into GIS. These spatio-temporal data
of landscape structure can be compared with existing spatial vegetation models (e.g. Jeltsch et
al. 1999) and linked to spatially explicit population models under varying land use and
climate change scenarios. In this context a new model focuses on the population dynamics of
Grewia flava, a common shrub in the southern Kalahari. Cattle-induced shrub encroachment –
an ecologically and economically important form of land degradation in savannas - is usually
explained in terms of livestock grazing altering shrub-grass competition in favour of shrubs.
However, empirical studies and modelling results show that under grazing of domestic
livestock shrub seed dispersal in cattle dung has a significantly higher influence on Grewia
encroachment. This has important implications for land use and management since, once
young shrubs have established, encroachment is almost irreversible.
Species diversity: - To understand the impact of climate change and land use on
species diversity both directly and via structural diversity, several target species were selected
that depend on different elements of vegetation structure (trees, shrubs) on different spatial
scales. The target species include animal species (tawny eagle Aquila rapax, small carnivores
e.g. yellow mongoose Cynictis penicillata, tree rat Thallomys nigricauda, sociable weaver
Philetairus socius and others) and plant species (Grewia flava, Lycium sp. and others). For
these species field studies on habitat use, population dynamics and population genetics will be
linked with population simulation models. For the tawny eagle first model results show that
the viability of the Kalahari-population is directly dependent (i) on the mean of annual rainfall
and (ii) on the variability and chronological order of rainfall. It is therefore suggested that
possible climatic changes will have a major effect on the survival of this population. Future
model investigations will show the effect of changes in vegetation structure (mainly large
nesting trees), on the survival of the population. For sociable weavers a metapopulation model
has been built to investigate (i) the effect of the spatial distribution of rainfall and (ii) the
effect of changes in size and distribution of trees on this population. Similarly to the tawny
eagle, preliminary results suggest a strong dependence on rainfall patterns. First results of the
field investigations on tree rats show a dependence of the population distribution as well as of
the individual behaviour of animals on the spatial structure of trees and shrubs. Further
investigations on population dynamics and on population genetics were started and data will
be used in a simulation model to show the effect of tree- and shrub structure on the
population. In a new study on the effects of land use on the biodiversity of small carnivores in
three different grazing regimes (cattle vs. sheep, vs. game) first results show, that yellow
mongooses strongly depend on vegetation structure: they dig their sleep and reproduction
burrows preferably under shrubs of Acacia hebeclada and Rhigozum trichotomum (>10 m
diameter), whereas shrubs of Acacia mellifera were rarely used. Density and distribution of
shrubs with an equivalent size differ between the three grazing regimes. The aim of our
further study is to investigate the consequences of these observed differences on the
composition of the small carnivore guild. Data on vegetation patterns show the importance of
Acacia trees on the overall plant species diversity and abundance: the number of plant species
and total vegetation cover were always higher underneath Acacia trees than in tree
interspaces. In a comparison between three sites of different land use intensities the effect of
Acacia trees were strongest at the unmanaged site. Increasing land use intensity was
correlated with a decline in species/cover of several physiognomic categories. Experimental
approaches to test which factor - shading, nutrient enrichment or dispersal - is limiting the
occurrence were started with a shrub, Lycium sp. First results on shading and nutrient
enrichment have shown that germination rate depends on nutrients. Linking of individual
population models with models of large scale vegetation dynamics is in progress.
Reference:
Jeltsch, F., Moloney, K.A. and Milton, S.J., 1999. Detecting process from snap-shot pattern: lessons
from tree spacing in the southern Kalahari.- Oikos 85, 451-467
117
Project ID: 01LC0024 (BIOTA Southern Africa S10)
01.10.2000 – 30.09.2003
WHICH PROCESSES AND MECHANISMS DETERMINE THE BIODIVERSITY IN
(SEMI-) ARID PLANT COMMUNITIES?
B. Reineking and C. Wissel
Department of Ecological Modelling, UFZ-Centre for Environmental Research Leipzig-Halle,
P.O. Box 2, D-04301 Leipzig
bjorn@oesa.ufz.de
Key words: allocation, allometric scaling, coexistence, competition, generic plant model,
individual-based simulation model, plant functional types, plant species diversity, spatial and
temporal pattern, stochastic environment, trade-off
Abstract:
The arid winter-rainfall region of the western Richtersveld (South Africa) harbours an
unusually high plant species richness, with maximum values of more than 40 perennials per
100 m2 [1]. Species coexistence in this system has been attributed to several factors, including
the plants’ relatively short life span, the high spatial and temporal heterogeneity in
environmental conditions, notably water availability, and the compartmentalisation of the
shallow soil by rocks, which reduces the spatial scale of competitive interactions between
plants.
The aim of the project is to assess the relative importance of these and other factors, using an
individual-based, generic plant model. Simulated spatial and temporal dynamics of the plant
community under different scenarios are compared with those observed in long-term field
studies, and with predictions from general theories of biodiversity.
RESULTS:
In generic plant models, different species are distinguished by only a few characteristics (2-4).
In particular, these are the biomass allocation patterns to different compartments such as
roots, stem, leaves, seeds and storage tissue. Other characteristics, such as seed size or
dispersal distance, may be included. The distinguishing characteristics form a lowdimensional ‘design space’. ‘Species’ are points within this design space and may be best
interpreted as representations of plant functional types.
The costs and benefits of biomass allocation to different compartments, in terms of water and
photosynthate gain or loss, are based on allometric scaling relationships (5). A sub-model is
developed of the dependence of occupied soil volume and water uptake on root biomass.
Model results include characteristic size and abundance distributions of different plant types
given specific environmental conditions (cf. 6).
The analysis of the simulation model has two main points of reference: general theories of
biodiversity on a more abstract level, and long-term field studies on a more concrete level.
Several ‘unified’ or ‘general’ theories of biodiversity and competitive coexistence have
recently been proposed (7, 9), each emphasising different possible mechanisms. The
simulation model’s dynamics will be interpreted in the light of these theories to assess the
degree to which they bear on the specific system under study.
The project’s empirical basis are long-term observations carried out by Norbert Jürgens and
co-workers over the last 20 years, in which location, size and number of fruits of individual
plants have been recorded on permanent plots in the western Richtersveld (South Africa).
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By mapping real species’ allocation patterns into the model’s plant design space, the model
results can be directly compared to the specific fine scale patterns observed in the western
Richtersveld region. However, because of the model’s generic structure, key findings of the
study are likely to generalise to other ecosystems of similar environmental conditions.
REFERENCES:
1. Jürgens, N., Gotzmann, I.H. and Cowling, R.M., Plant Ecol., 1999, 142, 87-96.
2. Tilman, D., Plant Strategies and the Dynamics and Structure of Plant Communities, 1988,
Princeton University Press.
3. Kleidon, A. and Mooney, H.A., Glob. Change Biol., 2000, 6, 507-523.
4. Schwinning, S. and Ehleringer, J.R., J. Ecol., 2001, 89, 464-480.
5. West, G.B., Brown, J.H. and Enquist, B.J., Nature, 1999, 400, 664-667.
6. Enquist, B.J. and Niklas, K.J., Nature, 2001, 410, 655-660.
7. Chesson, P., Theor. Popul. Biol., 2000, 58, 211-237.
8. Hubbell, S.P., The Unified Neutral Theory of Biodiversity and Biogeography, 2001, Princeton
University Press.
9. Pachepsky, E., Crawford, J.W., Bown, J.L. and Squire, G., N
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Project ID: 01LC0024 (BIOTA Southern Africa S11)
01.10.2000 – 30.09.2003
SOCIO-ECONOMIC ASPECTS OF CHANGES IN BIODIVERSITY IN SOUTHERN
AFRICA
Buss, H.-J., Falk, T., Heimpel, B., Hoffmann, I., Isaacs, G., Kirk, M., Knight, R., Krug,
M., Nuppenau, E.-A., Schneiderat, U., Werner, D.
Contact: Dept. of Livestock Ecology, Justus-Liebig-University Giessen, Ludwigstr. 21, 35390
Giessen: Irene.Hoffmann@agrar.uni-giessen.de; http://www.uni-giessen.de/ghi5
Key words: household, communal and commercial farming, livestock, game, rangeland
management, natural resources, agro-biodiversity, property rights, new institutional economics,
resource economics
Abstract:
The study aims at giving an overview over past and present livestock and rangeland management
strategies in communities and on commercial farms along the BIOTA transect. It also focuses on
institutions ruling the management. About 60 households in three rural communities and 20 farm
owners in two locations in South Africa and Namibia have been interviewed by structured and
semi-structured interviews. RRA tools were used in the communities. Preliminary results indicate
that
- livestock production systems differ along the transect, depending on the environmental
conditions, technologies applied, as well as on cultural background and traditions of the
different groups,
- the production systems have flexibly adapted to changing environmental, economic and
political conditions,
- water access, water quantity and quality are identified as a key problem for people living in
rural areas (esp. communal),
- rangeland quality with respect to bush encroachment is an issue for commercial farmers, who
take countermeasures, whereas livestock owners in communal areas do not actively react on
changes in vegetation composition.
Results:
Activities of BIOTA S11 started from July 2001 in the four sub-projects (SP)
1. identifying and analysing the actual land use practices and animal husbandry systems,
2. identifying suitable management practices for bio-economic models and rangeland policy,
3. assessing property rights over resources, organisational structure, and national tenure
policies,
4. assessing the economic value of savannahs and biodiversity with respect to human use.
Research methods applied included structured and semi-structured interviews, and interviews
with key persons.
SP1 and SP3 did their research jointly in three rural communities (Mutompo; Okamboro,
Soebatsfontein). About 40 households were interviewed in a full census in the first two villages,
and 50% of all 52 households in the last village. RRA tools such as, resource mapping, and group
discussion were applied.
Population density is high in all three villages. Household composition showed a high proportion
of old people and children below school age, particularly in Okamboro. Livestock holding is a
120
secondary enterprise, since most of the household income is derived from government transfers or
waged labour. Livestock holding differs, since the Herero and Kavango keep mainly cattle,
whereas the Nama/Damara keep small stock. Extensive livestock keeping in the communities is
less productivity- or market- than security- and savings-oriented. Data on the production of local
small ruminants were obtained in progeny histories. The body weight and body measurements of
sheep and goats of different ages were taken in more than 20 herds. Most animals were found to
be crossbreeds.
A long-term rangeland assessment tool is being developed. It aims at assessing the quality of
communal rangeland and at recording the changes from a scientific and farmers' point of view.
Local institutions that regulate access to and use of natural resources characterise all communities,
however, the importance of traditional vs. modern institutions and organisations varies
considerably. Local committees as resource allocating and sanctioning authorities have usually
been implemented by modern organisations or the state. In all communities, former common
property resources are partially privately appropriated and fenced in. The extent of this is mapped
by GPS and will be integrated in a GIS analysis in future. Migration and absentee livestock
ownership is of increasing importance.
SP 2 interviewed farm owners in three locations (18 around Erichsfelde, 3 in the Kalahari, 2
around Fish River Canyon) using a questionnaire on farm practices. Cattle production is the most
important enterprise around Erichsfelde, but tourism contributes to up to 50 % of the income.
Farmers notified overgrazing and bush encroachment as problems and take countermeasures.
Small ruminant production is most prevalent in the two locations in Southern Namibia. All
farmers try to make their production more extensive, to lower production costs, and to simplify
management.
Data on hunting and tourism operations were also collected on farms and integrated in a Linear
Programming model (first LP). It covers range management of wildlife and domestic herbivores.
It is temporal, i.e. covers overlapping resource use of several periods, is able to depict the impacts
from current grazing on future range quality, and integrates sound practices.
SP 4 is conducted in co-operation with the Dept. of Botany, University of the Western Cape. The
project focuses on 4 protected areas (Richtersveld, Namaqualand (Soebatsfontein community),
Rocherpan and Riverlands). All stakeholders, such as park management, adjacent commercial
farms and communities, will be interviewed to develop an inventory for the consumptive and nonconsumptive use of the natural resources in these areas. An online questionnaire will be set up for
internet users (especially park managers); tourists and locals will be interviewed by structured
questionnaires. A trial questionnaire has been forwarded to a flower tour operator for distribution
to tourists (clients) and the data collected. Specific economic valuations methods will be applied.
Aspects of the landscape will be modelled within a GIS and future scenarios generated.
Besides the research agenda, contacts to local organisations, extension agents, and research
partners were made, covering in addition the Richtersveld and Paulshoek area. A vivid exchange
with DRFN, NEPRU, the UNAM (Law Dept.) in Namibia, with the Dept. of Plant Conservation
of UCT and with Dept. of Agricultural Economics and Range Ecology, Orange Freestate
University Bloemfontein, already exists and is further deepened.
References:
1. Hoffmann, I.: The BIOTA project. Paper read at the Namibian Rangeland Forum, Okahandja,
28.6.01
2. Hoffmann, I., E.-A. Nuppenau, M. Kirk, R. Knight, U. Schneiderat, H.-J. Buss, T. Falk, G. Isaacs:
Socio-economic aspects of changes in biodiversity in South Africa. Poster, Arid Zone Ecology
Forum, Calitzdorp, 4.-7.9.2001
3. Isaacs, G., R. Knight, I. Hoffmann, T. Leymann: Protected areas. Evaluation techniques for
assessing socio-economic impacts for local communities. Poster, Arid Zone Ecology Forum,
Calitzdorp, 4.-7.9.2001
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Project ID: 01LC0007
1.10.2000-30.09.2003
BIOLOGICAL STUDIES ON MYCO-HETEROTROPHIC PLANTS WITH SPECIAL
EMPHASIS OF THEIR MYCORRHIZAE
Thassilo Franke 1) and Reinhard Agerer2)
Ludwig-Maximilians-University, Faculty of Biology, Department Biology I, Biodiversity –
Section Mycology, Menzinger Straße 67, 80638 München
1)
thafra@botanik.biologie.uni-muenchen.de , 2) myrrhmyk@botanik.biologie.uni-muenchen.de
Key words: myco-heterotrophic plants, arbuscular mycorrhiza, anatomy, Cameroon
Abstract:
The project is carried out in the Southwest Province of Cameroon. 12 species (7 genera; 4
families) of myco-heterotrophic plants could be recorded. A new species of Gymnosiphon
(Burmanniaceae) is described. All species were analysed morphologically and anatomically.
Mycorrhizal structures were observed. “Trap cultures” were established, using Hieracium
pilosella L. (Asteraceae) as a mycorrhizal host plant. “In situ” germination tests were carried
out. Observation plots were established near Limbe. Soil sampling, as well as plant monitoring
started in October 2001.
Introduction:
The aim of the project is the investigation of the poorly known biology of myco-heterotrophic
plants. Myco-hetrotrophic plants receive all essential nutrients (including carbohydrates) from
endomycorrhizal fungi, which are colonizing their roots and/or rhizomes [1]. The lack of
leaves and chlorophyll, as well as their subterraneous habit lead to the assumption that they
live as parasites on their associated fungi [2]. In tropical Africa comparatively few species of
myco-heterotrophic plants occur. An exception are glacial refuges like e.g. the rain forests
around Mt Cameroon in Cameroon’s Southwest Province, which are housing one third of all
African species of myco-heterotrophic plants [3].
Data concerning the ecology of myco-heterotrophic plants are very scarce and in the case of
most species - especially those from Africa - not existent. The main goal of this project is the
registration of the species composition of myco-heterotrophic plants in South-west Cameroon,
as well as the investigation of the mycorrhiza and the determination of host fungi.
Results:
Since the beginning of the project in October 2000, three trips to Cameroon were made. Two
trips were undertaken during the declining rainy season, that comprises the flowering season
of all myco-heterotrophic plants (orchids excluded). The main mission was the registration of
myco-heterotrophic plants in the Southwest Province. For this reason the type localities of
several species were visited. The rainforests that formerly covered the hills around the village
of Moliwe turned out to be completely replaced by oil palm plantations. This area is the type
locality of Afrothismia pachyantha Schltr. (Burmanniaceae) and Oxygyne triandra Schltr.
(Burmanniaceae). As the screening of all nearby patches of remaining forest was without any
success, it is to be feared that Oxygyne triandra, which is only known from a single
collection, became extinct. The type locality of Kupea martinetugei Cheek ined.
(Triuridaceae), that also comprises the only presently known population of A. pachyantha
(discovered by S. Williams in 1993 [3]) is highly threatened to be transformed into farmland.
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The presumably endemic K. martinetugei could still be found in abundance. In the Ekundu
Kundu community forest near Mundemba a new species of Gymnosiphon (Burmanniaceae)
was discovered and will be described soon. The taxonomical situation of the African species
of Gymnosiphon turned out to be very confusing. The careful study of type material might
reveal, that another two species (both collected near Mundemba) are new to science.
Altogether 12 species (7 genera; 4 families) of myco-heterotrophic plants could be recorded.
All detected species were analyzed morphologically and anatomically using light- as well as
electron microscopical (SEM, TEM) techniques. With regard to root anatomy, attention was
mainly paid to the endomycorrhizal structures of the involved host fungi. The mycorrhizal
patterns of all plants, except Auxopus macranthus Summerh. (Orchidaceae), showed the typical
characters of an arbuscular mycorrhiza. In order to determine the associated fungus, ‘trap cultures’
were established, using Hieracium pilosella L. (Asteraceae) as a mycorrhizal host plant. Root
fragments of Afrothismia winkleri (Engl.) Schltr. (Burmanniaceae), Burmannia congesta (Wright)
Jonk. (Burmanniaceae) and Sciaphila ledermannii Engl. (Triuridaceae) were placed onto the
living roots of H. pilosella. As arbuscular mycorrhizal (glomalean) fungi have no host specificity,
it is expected that the hyphae will grow over to the roots of H. pilosella. Later on the fungi will be
determined by molecular-biological comparison of isolated spores (extracted from ‘trap-cultures’)
and the fungal hyphae within the root tissues. Further on, it is planned to trace the host.-fungus in
the roots of adjacent photo-autotrophic plants, with which they perform mutualistic
endomycorrhizal relations. This also demands a careful screening of the surrounding photoautotrophic plant species. For this reason observation plots were established in BimbiaBonadikombo community forest near Limbe. Staff members of the Limbe Botanical and
Zoological Garden (LBZG) are carrying out a one-year plant monitoring that started in October
2001. Besides this, the abiotic environmental conditions are going to be investigated, especially
those concerning the physical and chemical soil composition of the rhizosphere. Soil samples that
had been taken at the plots were analysed in the soils research laboratory / Ekona, a facility of the
‘Institut de la Recherche Agricule pour le Development’ (IRAD). As the reproduction biology,
ontogeny and phenology of African myco-heterotrophic plants is completely unknown, several
field experiments are performed. “In situ” germination tests are carried out to investigate the
ontogeny. For this reason cell sieves (Falcon®), were provided with seeds of e.g. B. congesta and
buried in the soil. After six months some of the sieves were removed from the soil and examined.
The seeds were still vital but no germination process was observed. After 12 months only one
seedling of B. congesta could be detected within the sieves.
References:
1. LEAKE J (1994) The biology of myco-heterotrophic (saprophytic) plants. In Tansley Review
No.69, New Phytol. 127: 171-216.
2. RAYNAL-ROQUES A. & P ARE, J. (1998) Biodiversité des Phanérogames parasites: leur place dans
la classification systématique. Adansonia, sér. 3, 20 (2) : 313-322.
3. CHEEK M. & CABLE S. (1997) Plant inventory for conservation management: the Kew-Earthwatch
programme in Western Cameroon, 1993 – 96. Proceedings of the Limbe Conference Limbe
Botanic Garden, Cameroon 17-24 January 1997: 29 – 38.
123
Project ID: 01LC0017 (BIOTA West Africa)
01.01.2001 – 30.12.2003
BIOTA WEST AFRICA – GENERAL SUMMARY
a) Overall goals
BIOTA West Africa consists of ten subprojects that are part of the joint project BIOTA AFRICA.
Each of the three main BIOTA projects aims in its complimentary subprojects at assessing
• dimensions,
• spatial and temporal patterns and
• functional roles
of biodiversity in some of the most important African ecosystems.
The special focus in the upcoming main phase of the BMBF’s BIOLOG programme will be on
the anthropogenic change of biodiversity in recent times, both in its continent-wide impacts as
well as its profound regional peculiarities. The dramatic change is correlated with
• large scale habitat conversion and habitat destruction,
• overuse and mismanagement of natural resources,
• and is also affected by the summed spectrum of small scale human interferences with
the environment.
To design advanced management tools, it is necessary to know the status quo ante, compare it
with the current situation and comprehend the already detectable direct and indirect, immediate
and long-term consequences of the change on the ecological and socio-economic stage from the
local to the sub-continental scale in West Africa. Such tools will be better suited than any of the
presently available management measures for conserving the local and regional biodiversity and
for preserving the ecosystems’ functional integrity. These scientifically designed tools should
allow us to develop wiser and more sustainable forms of use of both the remaining natural as well
as converted ecosystems. These goals shall be reached by
• concentrating research on a limited geographical area (see Fig. 1, page 15),
• posing convergent questions and
• applying a harmonised methodology.
The closely aligned, co-operative and interdisciplinary research programme of BIOTA-West
integrates the following research areas: remote sensing, meteorology, biogeography, botany
(cross-cutting over different fields among those ethnobotany and archaeobotany), zoology (with
strong emphasis on entomology, herpetology, chiropterology, and limnology), different
subdisciplines of ecology, ethnology, archaeology, socio-economics.
b) Research localities and infrastructure
The studies of the Frankfurt-Group (W11) are being executed in Burkina Faso and in Benin with
their counterparts at the Université de Ouagadougou and the Université National du Bénin. A
further study (of Barbara Fruth), included under the umbrella of BIOTA-West, will conduct its
field work in the Republic of Congo. The main geographic focus within BIOTA-West is,
however, on Côte d’Ivoire, with the Centre de Recherche en Ecologie (CRE, Université de Abobo
Adjamé) and the Department of Botany at the Université de Cocody as the main counterpart
institutions in the country. In Côte d’Ivoire, six of the eight subprojects working in the field are
carrying out their empirical studies. During the first year the permanent research camp of the
University of Würzburg (originally financed by the Volkswagen Foundation) has served as the
already fully functioning infrastructural and spatial pivot, allowing some of the projects a flying
start. In due course the newly constructed and now nearly completed permanent Ivoro-German
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Field Station in the Comoé National Park will replace the University of Würzburg Field Camp as
one of our main infrastructural centres. This new research station will provide excellent working
conditions for both, demanding field studies as well as ambitious laboratory research. The station
is predominantly financed by (1) a single grant for the construction and most of the general
technical and laboratory equipment by the Fritz Thyssen Foundation, by (2) valuable
contributions to the infrastructure by the BMBF in the framework of BIOTA West, and (3) by a
long-term commitment by the Free State of Bavaria and the University of Würzburg to cover
some of the basic running costs. In addition to BIOTA-West, some of the BIOTA-East projects
are using this Research Station for comparative investigations. The rights and obligations of the
co-operating parties have been stipulated in an exchange of diplomatic notes between the
governments of the Federal Republic of Germany and the Republic of Côte d’Ivoire, dated March
3,1997.
The Comoé Research Station allows the lodging and boarding of up to twenty researchers at a
given time. There is enough space and all the basic equipment in the laboratory building to allow
all accommodated users to perform their research efficiently. Apart from the proper Research
Station inside the Park an Information/Education Centre is being built at one of the entrances of
the Park. It will serve public relations and educational purposes in a very broad sense and on very
different levels (from primary school level to advanced students and scientists) for both, visitors
of the Park and residents living in the Park’s wider vicinity. Through mediating educationadequate knowledge a widely lacking comprehension for the value of natural resources, for the
vulnerability of the fragile web of life, the necessity of nature conservation and of a wise and
sustainable use of natural resources shall be roused in the local population and far beyond. To this
end much valuable scientific contributions are expected from the BIOTA West projects.
c) The basic natural setting for BIOTA-West’s research
There is a steep climatic gradient prevalent along the north-south transect in the investigated part
of West Africa (see map, Fig. x), between the arid southern fringes of the Sahara and the humid
rain forests on the Atlantic Coast. This offers especially interesting research opportunities
regarding a number of aspects of biodiversity alterations within the framework of anthropogenic
global change. Most of our subprojects utilise this gradient, at least partly, by focussing on local
and/or regional patterns of diversity in different taxa. Comparative studies along this gradient will
allow the retrospective understanding of local and regional changes as a consequence of previous
natural climate variability within the passed millennia as well as predicting some probable
changes in the future under anthropogenicly altered conditions.
d) Understanding the natural background – a highly important objective
All the human-driven ecological changes can only be clearly identified against
• the background of the original patterns of biodiversity, and against
• the dynamic processes in the respective ecosystem not yet under strong pressure of
modern man.
Since the necessary previous knowledge that is needed here is not available at present for any of
the investigated biomes, basic research requires most of the room in the preparatory phase of the
first three years of BIOTA Africa in general and BIOTA West in particular. Such knowledge that
can nowadays only be acquired by conducting research in least disturbed systems within protected
areas is absolutely unrenouncable. Only with a sound scientific understanding of natural structures
and dynamics will we be able to achieve the already mentioned pre-eminent goal of mitigating
and eventually bringing to a halt further losses of biodiversity. Also for the additional goal of
giving recommendation for enhancing and broadening the wise and sustainable use of organismal
125
diversity, as claimed by the Convention on Biological Diversity (UNCBD), much basic research
is required.
The efficacy of all management measurements, be it for conservation or the ameliorated use of
biodiversity, will be scrutinised via long-term monitoring. This monitoring will also serve to
detect further changes of biodiversity and to recognise their consequences at the earliest possible
stage and help to clarify their causes. The latter, however, requires to distinguish natural
environmental variability from anthropogenicly caused alterations. Also this task cannot be
achieved without a sound knowledge of the natural systems’ structural and functional properties.
Given the high temporal variability in all natural systems, only long-term research will allow us to
gain the necessary insights. Time is running out for effectively curing the problems humans have
already inflicted on the worlds ecosystems in general and those of the African continent in
particular. We cannot continue to rely further on a pure trial and error strategy when searching for
the best means to effectively conserve and wisely use biodiversity.
e) Recognising and understanding biodiversity patterns on different spatial scales
BIOTA West is tackling its scientific problems on very different spatial scales –from
subcontinental to regional, landscape and to a more or less fine-grained local level. During the
first three years (the preparatory phase that will also serve for capacity building of our
counterparts and of our own working groups) we strive among others
• to find patterns of biodiversity at different spatial scales,
• to correlate them with other variables (e.g., climatic, edaphic, orographic, structural,
temporal), and
• to observe short-term processes that influence biodiversity patterns and dynamic
processes.
At the basis of such correlational data some modelling and up-scaling on larger areas that can only
punctually be checked by ground truthing is already possible. This holds especially true when
considering what in some instances older data (e.g., former floristic inventories – see the Biomaps
project –W03) and remote sensing are offering us as additional information. However, to
eventually understand the true cause-effect relationship, which is our final goal, experimental
analyses are required wherever possible. Such analyses have usually to be confined to small,
exemplary spatial and temporal sectors of the systems and yet are very time-consuming. Such
experimental studies have been commenced in a number of subprojects, but they will take their
time (and often will need to be extended into BIOLOG’s main phase) until one reaches first
conclusive results.
f) Achievements up to date
The project BIOTA West started in January 2001. Most subprojects needed some time for
practical preparations, for forming or modifying their institutional links, establishing their
infrastructure, etc. In the meanwhile this phase has been completed in all ten projects. Several
subprojects already started with their scientific work. The hitherto achieved results within all these
subprojects are mentioned in the following short abstracts, due to the very limited space, however,
only in sketchy form.
126
127
Project ID: 01LC0017 (BIOTA West Africa W01)
01.01.2001 – 31.12.2003
REMOTE SENSING AND GIS BASED SURVEY OF SPATIAL AND TEMPORAL
BIODIVERSITY DYNAMICS AND ANALYSIS OF BIODIVERSITY AND
GEODIVERSITY INTERRELATIONSHIPS
B. Hörsch, M. Vogel, C. Schultz and G. Braun
Deutsches Zentrum für Luft- und Raumfahrt DLR, DFD-UG, Linder Höhe, 51147 Köln-Porz
bianca.hoersch@dlr.de; www.dfd.dlr.de
Key words: Remote Sensing, GIS, Biodiversity-Geodiversity, Monitoring, Vegetation
Mapping, Capacity Building
Abstract:
This project shall create the basis for a system in which both, remote sensing and GIS
applications, form an analysis and monitoring system for the assessment of human impacts on
biological diversity. It is our aim to make the existent biodiversity more understandable and
predictable, taking into account the UN-Convention on Biological Diversity (UNCBD).
It is planned to establish a coherent GIS along the entire BIOTA transect in order to allow the
analysis of spatial patterns of biodiversity, its temporal changes and the mutual dependency
of geodiversity and biological diversity. Focus will lie on the comparison of natural
ecosystems with those where socio-economic activity has led to various degradation levels of
the former natural ecosystems. Furthermore, the detection of indications of spatial patterns of
diversity by remote sensing techniques will contribute to future monitoring and conservation
concepts.
Further tasks are the “capacity building” for co-operating partners within the fields of
Remote Sensing and GIS.
Results:
Within the last 10 months the data base of a Remote Sensing based GIS for the monitoring of
present-state biodiversity within the Western African study sites was built up [1]. In close
interaction with the project partners the different localities of field work were identified and
geographically referenced.
Tasks performed included first of all an investigation of appropriate multitemporal data sets from
commercial multispectral remote sensing systems (LANDSAT-7-ETM, IKONOS, CORONA
etc.). A set of LANDSAT-7-ETM scenes were acquired, covering the identified areas. All scenes
ordered were georectified to the common UTM system and were integrated as image base into the
GIS. Due to the lack of reliable topographical maps, Digital Chart of the World (DCW)-data were
used to integrate features like road networks, populated places and river networks into the spatial
database. These primary informations were further used to produce geometrically referenced
image maps which are essential as an auxiliary means for the orientation of the fieldworkers. They
also act as a base for mapping training samples which form the basis in the supervised
classification of vegetation patterns from the multispectral images [2, 3]. The maps were
enhanced by topological information (roads, settlements as far as available) and were
superimposed by a fine resolution latitude/longitude grid in order to facilitate orientation in the
field and to enable the direct comparison of GPS recordings with mapped features. The maps
were distributed to the project partners in paper printed form. On request, digital maps were also
supplied.
128
An important feature of a spatial database supporting biodiversity analysis in a more indirect way
are topographical data [4, 5, 6]. They form not only the basis for the absolute geographical
orientation of features in space, but are essential to derive topoclimatological information like
potential water and solar irradiance distributions in the terrain [7, 8]. Due to the lack of mid to fine
scale topographical maps, interferometric analysis of European Remote Sensing Satellite (ERS)tandem data has been started to derive Digital Terrain Models from the research areas in the Ivory
Coast and Benin.
Due to the importance of spatial climatic data for biodiversity modelling, research had been
carried out on downscaling spatially coarse climatic datasets in order to derive climatic
information on a spatial scale which is compatible to the scale of the research approach [9, 10].
Up to now, long term (1960-1990) precipitation and temperature data have been downscaled to a
spatial resolution of 5km and 2 km, respectively, as long-term monthly means. In the near future it
is intended to add additional climatic parameters and to cover the area with non-aggregated
monthly mean data.
To support the use of spatial data and information systems by the project partners two capacity
building activities took place at the DLR in Cologne and at the Institute of Botany in Hamburg.
Topics of the first course were primarily remote sensing basics with a special emphasis on sensor
sensitivity, reflectance of different surfaces, and generation and interpretation of false colour
composites. The second course held in Cologne focussed on Remote Sensing basics in
combination with GIS architecture for biodiversity research and monitoring [11]. Topics like
general data structure, the integration of referenced geospatial together with Remote Sensing data
and the main steps for building up of an information system were dealt with. Integration
possibilities of raster and vector based spatial data with ecological/biological attributes stored in
databases were addressed partially.
References:
1. Dale, V.H.; Offerman, H.; Frohn, R. & R. H. Gardner. (1995). Landscape characterization and
biodiversity research. In: Boyle, T.J.B. & Boontawee, B.: Measuring and Monitoring Biodiversity in
Tropical and Temperate Forests; Proceedings of a IUFRO Symposium held at Chiang Mai, Thailand
August 27. - September 2. 1994, Centre for International Forestry Research: Bogor, Indonesia.
2. Allner, T., Backhaus, R., Gaito, S. & J. McGlade: 1992: Biodiversity Indication by Earth
Observation Data. In: Proc. Of the Central Symp. of the Int. Space Year Coference, Munich, Germany.
30 March- 4 April 1992, 863-867.
3. Podolsky, R. H. 1990: Monitoring Biodiversity and Landscape Richness with digital Earth Imagery.
In: Archives of Photogrammetry and Remote Sensing Vol. 28, Part 7-1, Proceedings of the Symp. On
Global and Encironmetal Monitoring.
4. Burnett, M. R., August, P. V., Brown, J. H., & Killingbeck, K. T. (1998). The influence of
Geomorphological Heterogeneity on Biodiversity. A Patch-Scale Perspective. Conservation Biology,
12, 363-370.
5. Nichols, W. F., Killingbeck, K. T., & August, P. V. (1998). The Influence of Geomorphological
Heterogeneity on Biodiversity. A Landscape Perspective. Conservation Biology, 12, 371-377.
6. Wohlgemuth, T. (1998). Modelling floristic species richness on a regional scale: a case study in
Switzerland. Biodiversity and Conservation, 7, 159-177.
7. Antonic, O., Marki, A., & Krizan, J. (2000). A Global Model for Monthly Mean Hourly Direct
Solar Radiation. Ecological Modelling, 129, 113-118.
8. Wang, J., White, K., & Robinson, G. J. (2000). Estimating surface net solar radiation by use of
Landsat-5 TM and digital elevation models. International Journal of Remote Sensing, 21, 31-44.
9. Florinsky, I. V., Kulagina, T. B., & Meshalkina, J. L. (1994). Influence of Topography on
Landscape Radiation Temperature Distribution. International Journal of Remote Sensing, 15, 31473153.
10. Franklin, J. (1998). Predicting the Distribution of Shrub Species in Southern California from
Climate and Terrain-derived Variables. Journal of Vegetation Science, 9, 733-748.
11. ASPINALL, R. (1992). SPATIAL ANALYSIS OF WILDLIFE DISTRIBUTION AND HABITAT IN A GIS. IN:
P ROC. 5TH INTERNATIONAL SYMPOSIUM ON SPATIAL DATA HANDLING .
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Project ID: 01LC0017 (BIOTA West Africa W02)
1.1.2001 – 31.12.2003
BIOPHYSICAL AND HYDROMETEOROLOGICAL PARAMETERS WITHIN THE
FRAMEWORK OF TERRESTRIAL BIODIVERSITY RESEARCH
Dr. Jörg Szarzynski 1, Dipl. Geogr. Thomas Motzer 1 and Prof. Dr. Dieter Anhuf 2
1
Institut für Physische Geographie und Länderkunde, Universität Mannheim, L 9, 1-2,
D-68131 Mannheim
2
Instituto de Estudos Avançados, Av. Prof. Luciano Gualberto, Travessa J, 374, térreo, Cidade
Universitária, 05508-900, São Paulo, Brasil
j.szarzynski@web.de
Key words: abiotic environmental conditions, microclimate, energy budget, water balance
Abstract:
Since many ecological aspects of plants and animals are strongly related to local abiotic
conditions, measurements of meteorological and geophysical data provide comprehensive basic
information within the discussion of biodiversity stearing mechanisms. The scientific contribution
intends to link spatial and temporal variations of abiotic components within dry island forests and
savannas in Comoé National Park (Ivory Coast) to actual questions of biodiversity research.
Results:
Two representative experimental sites have been established within savanna and island forest
locations in order to determine relevant data concerning the energy- and water-budget, as well as
vertical profiles of micrometeorological parameters (Fig. 1). Furthermore, the analysis of digital
hemispherical photographs enables us to describe the structure of vegetation within the observed
formations (Fig. 2).
In addition, comparative studies will be conducted in the primary tropical rain forest in
southwestern Taï National Park. Comprising an area of 457.000 ha this location represents
one of the last large humid forest habitats in West Africa. Known as a centre of high
biodiversity the Taï Park was declared as a World Heritage Region by the UNESCO in 1982.
In the further course of the project the bioclimatological investigations will be carried out
simultaneously within dry forests/savannas in Comoé Park and evergreen forests of Taï Park in
order to analyse the largescale interannual precipitation variability and its impact on the different
vegetation formations.
Appendix:
130
Fig: 1: Installation of technical equipment at the savanna station
Fig. 2: Partly analyzed hemispherical photography with annual sun-tracks
131
Project ID: 01LC0017 (BIOTA West Africa W03)
1.1.2001 – 31.12.2003
BIODIVERSITY OF AFRICA IN THE GLOBAL CONTEXT: SPATIAL PATTERNS
OF VASCULAR PLANT DIVERSITY IN A CHANGING ENVIRONMENT
(BIOMAPS PROJECT)
J. Mutke, W. Küper, G. Kier and W. Barthlott
Botanisches Institut, Abt. Systematik und Biodiversität, Rheinische Friedrich-WilhelmsUniversität, Meckenheimer Allee 170, 53115 Bonn
Jens.Mutke@uni-bonn.de; www.botanik.uni-bonn.de/system
Key words: biodiversity mapping, biogeography, vascular plants, geodiversity, spatial analysis,
databases, GIS, information system
Abstract:
BIOTA BIOMAPS aims at the analysis of spatial patterns of African phytodiversity at regional to
continental scale. Using an inventory-based biodiversity mapping approach, a computer based
map of total vascular plant species richness for continental Africa has been generated (using
diversity figures for some 470 geographical units). For the first time, detailed statistical analyses
of the correlation of total plant species richness with climate, geodiversity and other abiotic
parameters are now available on the continental scale for Africa. Additionally, we computed the
first continental map of endemism richness. For more detailed analyses using a higher spatial and
thematic resolution, we are establishing a database on the distributions of selected plant taxa.
Until now, distribution data for about 1600 plant taxa are analysed. The established databases
provide a standardised data structure to make the scat tered data on floristics, biogeography, and
biodiversity of African vascular plants available.
Results:
As a baseline against which the results of more detailed analyses can be tested, we mapped and
statistically analysed spatial patterns of overall species richness of African plants on a continental
scale [1]. For these analyses, the complete datasets used by Lebrun [2] and Barthlott et al. [3, 4]
were revised, significantly expanded and reanalysed using an inventory-based biodiversity
mapping approach. The dataset currently includes 470 records of summary data taken from
literature on the phytodiversity of geographical units such as mountain ranges, national parks,
provinces, and others. Using a new methodological approach, we mapped endemism richness
values (an index combining endemism and species richness) for the seed plant flora of the 20
African biogeographical regions [5].
More detailed analyses were started using a taxon-based biodiversity mapping approach. The
respective dataset with distribution data for single taxa of African vascular plants allows not only
analyses with higher spatial resolution. It is as well the basis for more detailed studies of different
aspects of phytodiversity patterns such as endemism, phylogenetic diversity and complementarity
analyses. During the first months of the project the basic infrastructure for this approach was
established. Using the BioCise data model [6] a database for the management of plant distribution
data was developed and linked to GIS. Details of the database and possible co-operation are coordinated with W. Berendsohn (BIOLOG Biodiversity informatics). A continent-wide GIS on
high-resolution geodata (e.g., DEM, protected areas, climate, geodiversity and vegetation) was
established using internet-based information as well as data provided by BIOTA W01. These data
are used for the spatial analysis of plant distributions in relation to the abiotic environment to
allow predictive modelling of the probable range of a species. In addition, a Gazetteer with more
132
than 1 Mio geographical features on the African continent was compiled using different data
sources (NIMA, CIAT, etc.). This reference system facilitates the assignment of exact spatial coordinates to plant record localities. Using different online-databases (mainly the Kew Record of
taxonomic literature) a dataset on more than 5000 monographs and journal articles on taxonomy,
floristics, biogeography and phytosociology of African plants was established and revised for our
purposes. Extracts from the dataset were provided for other BIOTA subprojects, as well. Based on
our information about available plant distribution data, we are establishing a list of selected species, which are of interest considering our analytical purposes and the work of our co-operating
partners. For this work, we specifically co-operate with BIOTA sub-projects W04, W09, W11,
E04 and further taxonomic experts.
Until now, two main types of taxon-based data are analysed. The larger dataset at the moment are
dot maps of the distribution of some 1240 vascular plant taxa belonging to 124 genera digitised
from the Distributiones Plantarum Africanarum. The second dataset is based on the locality
descriptions of plant specimens cited in taxonomic revisions. Until now, data on some 420 taxa
from 19 plant families have been entered in the database. Furthermore, we are in contact with
specialists for various plant groups with the aim of joint analyses of their distribution data. First
plant groups to be analysed are Scrophulariaceae, Aristolochiaceae, Melinideae. In addition, we
are in contact with working groups (e.g., H.P. Linder, J. Lovett, P. Williams) doing comparable
analyses. Altogether, our taxon-based datasets currently include distribution data of some 1600
plant taxa. First analyses of diversity patterns for various plant functional types illustrate the
variety of analytical applications of this datasets [7].
References:
1. Mutke, J., Kier, G., Braun, G., Schulz, C., and Barthlott W. (in press), Patterns of African vascular
plant diversity - a GIS based analysis. Syst. Geogr. Pl.
2. Lebrun, J. (1960), Sur la richesse de la flore de divers territoires africains. Bull. Séances Acad. Roy.
Sci. Outre-Mer 6, 669-690.
3. Barthlott, W., Lauer, W. and Placke, A. (1996), Global Distribution of Species Diversity in vascular
Plants: Towards a World Map of Phytodiversity. Erdkunde 50, 317-327.
4. Barthlott, W., Biedinger, N., Braun, G., Feig, F., Kier, G. and Mutke, J. (1999), Terminological and
methodological aspects of the mapping and analysis of global biodiversity. Act. Bot. Fennica 162, 103110.
5. Kier, G. and Barthlott, W. (2001), Measuring and Mapping endemism and species richness: a new
methodological approach and its application on the flora of Africa. Biodiversity and Conservation 10,
1513-1529.
6. Berendsohn, W. G., Anagnostopoulos, A., Hagedorn, G., Jakupovic, J., Nimis, P. L., Valdés, B.,
Güntsch, A., Pankhurst, R. J. and White, R. J. (1999), A comprehensive reference model for biological
collections and surveys. Taxon 48, 511-562.
7. Mutke, J., Küper, W., Braun, G. and Barthlott, W. (2001), Analysing diversity patterns of different
plant functional types in Tropical Africa. Verhandlungen der Gesellschaft für Ökologie 31, 63.
133
Project ID: 01LC0017 (BIOTA West Africa W04)
1.1.2001 – 31.12.2003
PHYTODIVERSITY AND DYNAMICS OF HABITAT FRAGMENTS IN THE IVORY
COAST: SPATIAL AND TEMPORAL PATTERNS OF BIODIVERSITY CHANGES,
EDGE EFFECTS, AND THEIR FUNCTIONAL CONSEQUENCES IN NATURAL
AND DISTURBED ECOSYSTEM MOSAICS
Dethardt Goetze 1 , Annick Koulibaly2 , Klaus Hennenberg 1 , Rudolf Pivarci1 , Philippe
Kersting 3 , Dossahoua Traoré 2 , Stefan Porembski1 , Jörg Grunert 3
1
Universität Rostock, Institut für Biodiversitätsforschung, Allgemeine und Spezielle Botanik,
Wismarsche Strasse 8, D-18051 Rostock, Germany
2
Laboratoire de Botanique, UFR Biosciences, Université de Cocody, 22 B.P. 582, Abidjan
22, Côte d’Ivoire
3
Universität Mainz, Geographisches Institut, D-55099 Mainz, Germany
stefan.porembski@biologie.uni-rostock.de;
http://www.biologie.uni-rostock.de/abt/botanik/ botanik.html
Keywords : forest island, forest regeneration, forest-savanna ecotone, global change,
landscape structure, fire, soil properties, sustainable land management, socio-economical
conditions, modelling, vegetation map, remote sensing, GIS, Comoé National Park
Abstract:
In the forest-savanna mosaic of West Africa, habitat fragmentation caused by man leads to a
decline of natural forests, their increasing isolation, a growing influence of edge effects from
the surrounding savanna [1], and an alteration of species composition. As a result,
biodiversity, as the most important natural resource of the country and basic foundation for
the livelihood of the inhabitants, is declining. In a period of intensification of land use and
global change, the consequences of habitat fragmentation on biodiversity are being studied
with a focus on temporal and spatial patterns of biodiversity changes, as well as the
increasing significance of edge effects. The core questions deal with the consequences for the
ecological functioning of natural and cultivated ecosystems [2,3], the quantification of
landscape diversity, and the establishment of recommendations for a sustainable land use
aiming at a long-term preservation of biodiversity. For this purpose, field data are being
assessed in the Comoé National Park and neighbouring utilized areas by use of various
transect methods and the newly installed biodiversity observatories. They are being merged
with remote sensing data and processed to models using advanced statistical procedures and
GIS.
Results:
The analysis is subdivided into five thematic sections:
1. Spatio-temporal analysis of the development of habitat patterns and extrapolation of
the results to larger areas. Based on the analysis of new remote sensing data and intensive
field work, a map of the important vegetation types of the National Park is being generated. It
serves as a basis for the subsequent analysis of landscape structure and diversity. When
tentatively related to former data [4], spatio-temporal dynamics of the forest islands become
obvious; however, a consistent trend has not been detected yet. The predominance of certain
vegetation types in the respective forest islands shows that under natural conditions we may
reckon with high beta-diversity among particular forests, whereas the diversity of vegetation
types within a forest island is limited. Currently, the data of training areas are being
134
extrapolated onto larger areas within the satellite image in order to generate a GIS vegetation
map of the entire region through multiple ground truthing. (Cooperation with W01, W03,
W09, W11)
2. Analysis and modelling of edge effects between forest and savanna. Along selected
monitoring transects in forest islands of the National Park, botanical and structural relevés are
being collected, and the long-term influences of the factors grazing, soil disturbance, and fire
are being investigated by means of experimental interference. Furthermore, natural
regeneration of woody plants and herbaceous standing crop are being recorded, and
experiments on the establishment of transplanted seedlings and in-situ germination of selected
woody species are being carried out. Germination characteristics are being compared under
controlled laboratory conditions. The biotic parameters are being merged with the
determining parameters (microclimate, soil, structure, fire). Though a clear structural and
microclimatic gradient was shown to exist along the forest edges during the rainy season, the
transplanted seedlings of characteristic tree species showed the same survival rates and
relative growth during the first 2 months. Thus, the dynamics of forest edges are presumably
determined by the microclimatic conditions of the dry season as well as timing and intensity
of the fire events. (Cooperation with W02, W03, W06, W09, W11, S09)
3. Study of the land utilization regime in neighbouring areas of the National Park,
comparison of the consequences of habitat fragmentation in the utilized and protected
areas. In the cultivated areas, monitoring plots were established in close agreement with the
local inhabitants, and the botanical inventory was started. Currently, the vegetation structure
[5] and the occurrence of species groups indicating certain types of land use are being
analyzed in monitoring transects [6]. It becomes evident that due to utilization, forest edge
ecotones are becoming larger at the expense of the forest area. Hence, the effects of abiotic
parameters (microclimate, structure, soil, fire) are being investigated. On the basis of
extensive polls at local administrative offices and questionnaires of the population in the areas
under study, data are being obtained and analyzed on past and present land-use practices as
well as the socio-economic conditions of the inhabitants. This in turn will aid interpretation of
present and future ethnobotanical developments. Intensive capacity building is being
undertaken by an Ivorian PhD candidate working on this topic. (Cooperation with W01, W03,
W09, W11)
4. Study of the soil properties. Currently, catenas and chemistry of the soils of the area are
being analyzed in order to systematically quantify the impact of soil properties on the
formation of forest-savanna mosaics and on the regeneration of forests. (Cooperation with
W02, W06)
5. Comparative long-term monitoring of biodiversity changes at monitoring plots in
relation to anthropogenic factors. An observatory was installed each inside and outside the
Park by means of a DGPS. Its vegetation was assessed and classified in all 1 ha subplots [7].
The subplots to be analyzed by all working teams were determined by means of a stratified
ranking procedure. A detailed inventory of the vegetation of the subplots and of additional
permanent transects has been commenced. Another pair of observatories will shortly be
installed in an adjacent region. (Cooperation with W01, W02, W03, W06, W07, W09, W11)
References:
1. Forman, R. T. T., 1995: Land mosaics: the ecology of landscapes and regions. Cambridge.
2. Schulze, E. D. & Mooney, H. A., 1993: Biodiversit y and ecosystem function. Berlin a. o.
3. Walker, B. & Steffen, W. (eds.), 1996: Global change and terrestrial ecosystems. Cambridge.
4. Hovestadt, T., Yao, P. & Linsenmair, K. E., 1999: Plant Ecol. 144: 1–25.
5. Gautier, L., Chatelain, C. & Spichiger, R., 1994: XIIIth AETFAT Congress, Malawi: 1339–1350.
6. Koulibaly, A., 2000: Charactéristiques de la végétation et de la flore de la savane soudanienne de
Côte d’Ivoire: Cas des alentours de la ville de Korhogo. DEA. Univ. Cocody.
7. Trochain, J-L., 1957: Bull. Inst. Centrafrique N. S., Brazzaville, 13–14: 55–93.
135
Project ID: 01LC0017 (BIOTA West Africa W06)
1.1.2001 – 31.12.2003
DETERMINANTS
OF
SMALL-SCALE
MOSAICS
OF
ARTHROPOD
COMMUNITIES IN NATURAL AND ANTHROPOGENICALLY DISTURBED
HABITATS
Karsten Mody, Christian Kost, and K. Eduard Linsenmair
Lehrstuhl für Tierökologie und Tropenbiologie, Universität Würzburg, Biozentrum, Am Hubland,
97074 Würzburg
Ke_lins@biozentrum.uni-wuerzburg.de ; http://www.biozentrum.uni-wuerzburg.de/zoo3/
Key words: animal-plant-interactions, arthropod communities, community structure, Comoé
National Park, Dorylus, local processes
Abstract:
The diversity and structure of ecological communities is determined by many parameters. In
order to understand the influence local processes exert on the maintenance of biodiversity in
natural and anthropogenically altered environments, two arthropod systems are studied in the
West African Comoé National Park. In arthropod communities living on individual savanna
shrubs and trees, the role of deterministic small scale variation in plant and habitat
characteristics for community composition is investigated. Repeatedly sampled, the arthropod
communities of individual shrubs and trees were distinctive even for conspecific plants growing in
close neighborhood. Thus, each plant was inhabited by a unique, characteristic set of arthropods.
This striking pattern suggested a community organization governed rather by deterministic, small
scale environmental variables than by stochastic events. Focal observations on several arthropod
species belonging to different guilds supported this interpretation. Driver ants (Dorylus
nigricans) are studied as a stochastic disturbance of arthropod communities. Driver ants are
considered to be an important, community-structuring source of disturbance especially for
ground living arthropod communities. In a first analysis indirect evidence is presented that driver
ant raids reduce the abundance of some potential prey taxa as well as that of potentially
competing species.
dc
ls
A
B
C
Fig.1: A: Apparatus for standardized measurement of herbivory: area of leaf samples (ls) is quantified before and after
feeding trials using a digital camera (dc); B: Caterpillar of Chrysopsyche imparilis; C: Dorylus nigricans driver ants.
136
Results:
Repeatedly sampling complete arthropod faunas of individual plants revealed that savanna
shrubs and trees are inhabited by distinctive sets of arthropods which turned out to be stable
for – at least - more than a year. Such arthropod communities, unique for individual plants,
could be found for trees and shrubs exceeding a certain minimum size (Fig.2). This finding
could be confirmed even for conspecific plants growing in close neighborhood. Since the
species pool for colonization is identical for these plants, it has to be concluded that
deterministic habitat characteristics, acting on a restricted local scale, are influential on
community composition. Such deterministic habitat parameters could be variable plant
characteristics like plant morphology and chemistry or environmental variables like soil
heterogeneity (determined in coordination with W02 and W04), generating variation in
arthropod-relevant parameters on a local scale [1]. Thus, small scale variability in habitat
characteristics seems to be a crucial factor for diversity in arthropod communities. It may play
a key role in the interpretation of similarities and differences found for regions or sites
differentially influenced by humans and may have profound implications for management
measures.
0,24
±Std.Dev.
mean similarity [mean JI]
0,20
P < 0.0001
±Std.Err.
Mean
0,16
P = 0.68
0,12
0,08
0,04
0,00
-0,04
SC1
same plant
SC2
other plants
same plant
other plants
Fig.2 shows similarities in species composition of arthropod communities (excluding ants) eight times sampled from the
same and different Combretum nigricans plants respectively. It becomes clear that communities obtained from the same
plants are significantly more similar than communities from different plants when shrubs of Size Class 1 (SC1: plants with
175 to 1090 leaves) are considered. No difference in species similarity of communities was detectable for small plants of
Size Class 2 (SC2: plants with 23 to 94 leaves). Paired t-test; N per size class: 14 plants; JI = Jaccard Index.
The distribution of herbivorous insects seems to reflect the intraspecific variability of
plant characteristics mentioned above. This is, as an example, shown in the distribution of the
mobile scarabaeid beetle Apogonia fatidica and is probably causing food plant changing
behavior of less mobile moth caterpillars. A. fatidica occurred highly aggregated on certain
individuals of the savanna tree Combretum nigricans while it was completely absent on some
neighboring C. nigricans plants (Fig.3A). Feeding experiments (cf. Fig.1A) demonstrated that
this characteristic distribution pattern was tightly coupled with leaf attractiveness which
significantly varied between individual C. nigricans plants for the beetles (Fig.3B).
Caterpillars are limited in choosing between different plants and in reaching the most
attractive and best suited plants. However, some caterpillars feeding on C. nigricans change
food plants regularly. This change of food plants reduces – in view of highly variable plants the probability of using exclusively unfavorable plants and results in a diversified diet.
Feeding on different plant individuals can be positively correlated with fitness which was
experimentally shown for Chrysopsyche imparilis (Lepidoptera, Lasiocampidae)
(Figs.1B&3C).
137
70
9. Coll.
number of beetles
60
8. Coll.
50
7. Coll.
40
6. Coll.
5. Coll.
30
4. Coll.
20
3. Coll.
2. Coll.
10
1. Coll.
P14
P13
P12
P11
P9
P8
P10
A
P7
P6
P5
P4
P3
P2
P1
0
Plant ID
55
***
***
***
ns
**
***
***
***
35
25
15
uC 3
aC 3
uC 6
aC 6
uC 6
aC 6
uC 5
aC 5
uC 4
aC 4
uC 3
aC 3
uC 2
aC 1
-5
aC 2
5
uC 1
consumed leaf mass [mg dw]
45
Plant ID
B
200
±Std.Dev.
P = 0,03
±Std.Err.
Mean
160
egg number
120
80
n = 23
40
n = 20
C
0
single-plant food
mixed-plant food
Fig.3 A: Aggregated distribution of Apogonia fatidica beetles on 14 Combretum nigricans plants (P1-P14) which are
exemplarily presented for their regularly assessed beetle abundance. Nine consecutive collection events (Coll.) are shown.
Differing beetle densities on individual plants reflect variable plant characteristics (attractiveness). B: Comparison of leaf
attractiveness of paired C. nigricans plants (C) to A. fatidica beetles. Each plant pair tested in the lab contains a plant
individual which attracts high beetle densities in the field (attractive Combretum = aC) and a plant individual which attracts
only small numbers of beetles in the field (unattractive Combretum = uC). Significant differences in consumed leaf mass
(dw = dry weight) are indicated (paired t-test): ns = not significant; ** = P<0.01; *** = P<0.001; Box-Whisker-Plots:
Central line = Mean, Box = Std. Err., Whisker = Std. Dev.; N (per plant pair): 12 leaf pairs, 120 beetles. C: Comparison of
egg numbers laid by Chrysopsyche imparilis females raised either on leaves of a single Combretum nigricans plant (singleplant food) or on leaves of a mix of six C. nigricans plants (mixed-plant food). Females raised on the mixed diet laid
significantly more eggs. This indicates that changing food plants can be advantageous in view of variable plants and may
138
evidently enhance reproductive output
Driver ants (Fig.1C) occur in massive densities and show ferocious behavior when on hunting
raids. Therefore they are supposed to exhibit a strong effect on arthropod communities in the
leaf litter of the gallery forest, leading to the hypothesis that driver ants act as a diversityenhancing ‘intermediate disturbance’ [2]. Besides experimental evidence, two prerequisites
would, if well-documented, make this hypothesis plausible: That there is a patchwork of
arthropod communities in the leaf litter that is not determined by environmental conditions
(vegetation, geo-morphology, humidity, etc.), and that driver ant raids exhibit a relevant
disturbance on the arthropod communities. Not enough data have been sampled yet with
regard to the first question, but we can present some crude analysis of the effect of ant raids
on the abundance of the higher taxa of leaf litter arthropods (Tab.1). For Saltatoria, Hemiptera
(mainly Cicada), Collembola and the larval states of Holometabola a significant increase of
abundance could be documented during multiple sampling after ant raids (max. 16 days),
serving as indirect evidence that abundances are recovering after they were reduced by the
raids. For other groups it was not found, in some cases possibly because of small sample sizes
or a wrong time-frame (e.g. too short for ant colonies with main losses in the brood rather
than in the sampled workers). Interesting is the significant effect on the abundance of nonformicid Hymenoptera which consisted almost exclusively of minute parasitic wasps. This
may indicate a second order response on the ants via the availability of host species,
considering that the tiny, mobile wasps are probably not preyed upon heavily by the ants. The
existence of a succession of communities after ant raids is crucial to the proposed effect of
driver ants on species diversity in the region. The results presented here are only first cues to
assess what taxa are affected by the ants. An analysis of species identities in the samples will
help to collect further evidence for an influence of Dorylus on the edaphic species
communities.
Tab. 1 shows the effect of time after Dorylus-raids on the abundance of several groups of leaf-litter arthropods. Six (pit-fall
traps), respectively five (Winkler samples) sites were sampled 0, 1, 2, 4, 8, at some sites up to 16 days after ant raids
(unfortunately, critical before-raid samples can often not be obtained due to the unforeseeable nature of the raids). Trends
are indicated as (+/0/-), R-values of significant Spearman-rank correlations are given. Note that in some taxa opposite
trends occurred at different sites which requires a more detailed analysis.
Taxon
Annelidae
Blattodea
Coleoptera
Collembola
Diptera
Formicidae
Hemiptera
Hymenoptera (non-Formicidae)
Isoptera
Larvae (mainly Coleoptera)
Millipedes
Mites
Saltatoria
Spiders
Pit-fall traps
0
ns
+
ns
+
ns
ns
+
ns
+
ns
+*
R=0.412
+
ns
0
ns
0
ns
0
ns
0
ns
+*** R=0.567
0
ns
Winkler-samples
+
ns
+
ns
0
ns
+*
R=0.390
+
ns
0
ns
+
ns
+*
R=0.315
0
ns
+*
R=0.315
0
ns
0
ns
+
ns
0
ns
References:
1.
2.
Fritz, R.S., Simms, E.L., editors. Plant Resistance to Herbivores and Pathogens: Ecology,
Evolution, and Genetics. Chicago: University of Chicago Press; 1992. 590 p.
Connell, J.H. Diversity in tropical forests and coral reefs. Science, 1978, 199, 1302-1309.
139
Project ID: 01LC0017 (BIOTA West Africa W07)
01.01.2001-31.12.2003
COMPARATIVE STUDIES ON EPHEMERAL SAVANNA WATERS AND THEIR
SIGNIFICANCE
FOR
DIVERSITY
AND
PRODUCTIVITY
OF
FISH
COMMUNITIES IN NATURAL AND ANTHROPOGENICALLY DISTURBED
RIVERS
A. Kahlheber, M. Boutros and K. E. Linsenmair
Lehrstuhl für Tierökologie und Tropenbiologie, Zoologie III, Universität Würzburg,
Biozentrum, Am Hubland, 97074 Würzburg
ke_lins@biozentrum.uni-wuerzburg.de; http://www.biozentrum.uni-wuerzburg.de/zoo3/
Key Words: Comoé National Park, ephemeral waters, fish communities, lateral migration, local
processes, spatial-temporal utilization patterns
Abstract:
Freshwaters in tropical savanna biomes are currently subject to massive changes.
Deterioration of water quality, elimination of whole spectra of habitats and profound
alterations in the basic hydrological conditions are especially serious consequences of human
activities. In order to understand the natural organization of local fish assemblages and to
assess the effects of anthropogenic influences on the composition and functional
characteristics of fish communities, the fish fauna of undisturbed savanna waters in Comoé
National Park, Ivory Coast, and in disturbed adjacent areas is investigated. The aim is to
describe the spatial and temporal use patterns of savanna waters by age classes, species and
assemblages of fish and to understand the factors that are causing these use patterns. In the
first year of the project, the main emphasis is put on surveying the composition of fish
communities that use ephemeral savanna waters temporarily connected to Comoé river, the
only permanent water in the region. 65 species of fish have been caught in these waters so far,
among them some new to the fish community hitherto reported for Comoé river. The general
composition of fish assemblages of ephemeral waters is comparable to the fish community of
Comoé river. However, analyzing parameters of distribution more precisely, a species
specific, fine grained use pattern becomes evident. It points to the important role ephemeral
waters can play for many species of fish as feeding, growing or spawning sites.
Results:
Our first results indicate that ephemeral savanna waters are an important fish habitat although they
are useable only for a limited time of the year. A comparison of the fish fauna of the great river
systems of Ivory Coast (Sassandra, Bandama, Comoé) and the fish assemblages of our study area
shows that most fish locally occurring in permanent waters can also be found in ephemeral
waters. While 91 species of fish are reported for the whole Comoé river system (length: 1,160 km;
drainage area: 72,000 km²) [1,2], 65 species of fish could already be recorded in the extremely
limited savanna waters studied so far (three 200 m sections of two ephemeral savanna rivers and
fifteen ponds with a total surface area of less than 1 km²).
An analysis of assemblage composition indicates that use of ephemeral habitats is not
restricted to certain groups but is to be found in a wide range of taxonomic groups of fish
(Fig.1). Simply the Cyprinodontidae seem to be relatively rare in ephemeral savanna waters in
comparison to the total fish fauna of Comoé river [3]. This might be caused by the existence
of many species in estuarine or rainforest waters which contribute to the total Comoé fish
fauna. On the other hand, we have recorded one annual cyprinodontid as a characteristic
inhabitant of ephemeral savanna ponds which previously has not been reported for Comoé
river. Another group of fish, the Cyprinidae, appear, however, especially species-rich in
140
ephemeral savanna ponds and streams. With the documentation of three species, new to the
Comoé fish fauna, more species of cyprinids are known from the ephemeral as compared to
the river community. This finding stresses the great importance of ephemeral savanna waters
to these fishes. It also points to gaps in the current knowledge of the distribution of many fish
species in the region. As all fish - with the exception of annual cyprinodontids and
Protopterus-lungfish which can survive dry seasons without permanent water as eggs or
buried in mud holes - have to newly re-colonize ephemeral savanna waters from permanent
waters, i.e. from the Comoé river in our case, they have to occur also in the river itself.
Ephemeral waters
Comoé
Cyprinodontidae
Cichlidae
6% (4)
8% (5)
Others
9% (6)
Cyprinodontidae
16% (15)
Siluriformes
18% (16)
Siluriformes
18% (12)
Others
11% (10)
Mormyridae
14% (9)
Cyprinidae
26% (17)
Cichlidae
9% (8)
Characiformes
18% (12)
Characiformes
18% (16)
Mormyridae
11% (10)
Cyprinidae
18% (16)
Fig.1: Taxonomic composition of fish assemblages of ephemeral savanna waters (our study) and a great West
African river, the Comoé (published data). Total species number for each group mentioned is given in brackets.
“Others” comprise: Anabantidae, Centropomidae, Channidae, Mastacembelidae, Polypteridae, Protopteridae
(for ephemeral waters) and Anabantidae, Centropomidae, Channidae, Clupeidae, Eleotridae, Mastacembelidae,
Notopteridae, Polypteridae, Protopteridae (for Comoé river).
Analyzing parameters of distribution and habitat characteristics more precisely (in
cooperation with W01, W02, W04), a species specific, fine grained use pattern of ephemeral
waters is becoming evident. In dependence of water availability, the composition of fish
assemblages in savanna waters changed. On the one hand, species of Barbus, Aplocheilichthys
and Hemichromis re-colonized ephemeral waters directly after first rains. They may either try
to migrate in savanna ponds far away from Comoé river or to take advantage of low predation
pressure by predatory fish in newly developing waters. On the other hand, fish like Brycinus
nurse, Labeo parvus or Schilbe spec. started lateral migration much later, entering savanna
waters at a time when water level remained high and rather predictable and stable food
sources had been established. The different patterns in utilization of ephemeral savanna
waters by a species-rich fish community point to the important role these waters can play for
fishes as feeding, growing or spawning sites.
References:
1.
2.
3.
Teugels, G.G., Lévêque, C., Paugy, D. and Traoré, K. État des conaissances sur la faune
ichtyologique des bassins côtiers de Côte d`Ivoire et de l`ouest du Ghana. Revue d`
Hydrobiologie Tropicale, 1988, 21, 221-237.
Livingstone, D.A., Rowland, M. and Bailey, P.E. On the size of African riverine fish faunas.
American Zoologist, 1982, 22, 361-369.
Lévêque, C., Paugy, D., Teugels, G.G. Faune des poissons d'eaux douces et saumâtres de
l'Afrique de l'Ouest, Tome 2. Tervuren and Paris: MRAC-ORSTOM; 1992. 902 p.
141
Project ID: 01LC0017 (BIOTA West Africa W08)
01.01.2001-31.12.2003
AMPHIBIAN DIVERSITY AND DIVERSITY CHANGES IN WEST AFRICA:
GLOBAL, REGIONAL OR LOCAL CAUSES?
M.-O. Rödel1, 2, 3, J. Kosuch2 , R. Ernst1 , V. Rudolf1 , G. Schorr1 , C. Harbig1 ,
S. Frohschammer1 , G. Leistner1 , M. Wegmann1 , P.J. Adeba 3 , G.N. Kouamé 3 , A.A.
Cudjoe 4 , K.E. Linsenmair1 and M. Veith2
1
Lehrstuhl für Tierökologie und Tropenbiologie, Zoologie III, Universität Würzburg,
Biozentrum, Am Hubland, D-97074 Würzburg, Germany; 2 Institut für Zoologie, Saarstrasse
21, D-55099 Mainz, Germany; 3 Université Abobo Adjamé et Université Cocody, Projet
Biodiversité, BP 20 Taï, Côte d’Ivoire; 4 Ghana Wildlife Division, PB M 239, Accra, Ghana
roedel@biozentrum.uni-wuerzburg.de; http://www.gadag.org; http://www.biozentrum.uniwuerzburg.de/zoo3/; http://perdix.biologie.uni-mainz.de/
___________________________________________________________________________
Key Words: Amphibia, bio-monitoring, community ecology, diversity, forest, Ghana, genetics,
Ivory Coast, primary habitats, savanna, secondary habitats, West Africa
Abstract:
Amphibian diversity along environmental and disturbance gradients in West Africa has been
investigated. Diversity was higher than expected and at least equals that of neotropical
regions. Habitat specific anuran communities could be characterized and basic data on life
history traits of possible target species for a long term monitoring program have been
compiled.
Results:
As a base line for our future research amphibian diversity throughout different West African
ecotones has to be documented. So far we compiled species inventories of five national parks
in Ivory Coast and the Ghanian Volta region (Fig. 1). By means of standardized sampling
techniques we obtained basic data on the amphibian community composition in different
habitat types (forest, savanna, primary, secondary). This enables us to detect diversity changes
over time, and/or along environmental and disturbance gradients.
In total 96 amphibian species were recorded from Ivory Coast. From 61 species DNA samples
have been collected and partly sequenced. For most of the species at least basic biological and
ecological data are now available. We were able to document several reproductive strategies
so far unknown for African anurans [4, 7], as well as unknown interactions of other groups of
organisms with amphibians [5]. By means of morphological, behavioral, acoustical and
genetic comparisons we detected five species new to science [6].
Analyses of the leaf litter anuran assemblage of Taï National Park by means of niche overlap
analysis, non-metric-multidimensional-scaling, and Mantel-test comparison revealed that the
observed multivariate structure is only weakly influenced by biotic interactions and therefore most
likely not the sole result of past or present species interactions. Results indicate that different life
histories are likely to explain the observed pattern. In primary forests, distinct species assemblages
existed but could be explained by geographic proximity and therefore the existence of common
local species pools. Assemblages in secondary forests were predictable based on environmental
parameters. This can be inferred to be the result of physiologically more restrictive conditions
within secondary forest habitats especially due to an altered microclimate. Only highly adapted or
tolerant species may be able to cope with these conditions.
142
These results lead to important implications for bio-monitoring and conservation management
and provide useful information, which is urgently needed to assist conservation efforts.
Surveys of the populations of selected species may yield information about the state of
disturbance of an area. We currently selected several anuran species for further investigations,
that proved to be specialized on certain habitat types and/or show peculiar life history traits
and therewith represent different ecotypes. These comprise three species with direct
development that are restricted to disturbed forests (Phrynobatrachus alticola) or occur in
varying abundances in primary and secondary forests (Arthroleptis variabilis, A. crusculum),
as well as three species that all maintain tadpole stages but reproduce in different habitats
[Phrynobatrachus guineensis: breeds in tree holes in drier parts of primary forest; Kassina
lamottei: breeds in large ponds in swampy parts of primary forest (Fig. 2); Cardioglossa
leucomystax: reproduces in slow running forest creeks].
Comoé, CI (35)
Marahoué, CI (31)
Lamto , CI (39)²
Mt. Sangbé, CI (42)
Volta Re., GH (32)
Mt. Péko, CI (35)
Tai, CI (56)
Mt. Nimba, LI (57)³
3,0
3,5
4,0
4,5
5,0
5,5
6,0
6,5
7,0
euclidian distance
Fig. 1: Similarity of West African amphibian
communities; presence-absence data, species
numbers in parentheses, CI = Côte d’Ivoire,
GH = Ghana, LI = Liberia, ² = [3], ³ = [1, 2, 8].
Fig. 2: Kassina lamottei an endemic frog of the
Upper Guinea forest zone, confined to primary
rainforest, drawing: M.-O. Rödel.
References:
1. Guibé, J. and Lamotte, M. La réserve naturelle intégrale du Mont Nimba. XII. Batraciens (sauf
Arthroleptis, Phrynobatrachus et Hyperolius). Mem. Inst. fond. Afr. noire, 1958, 53, 241–273.
2. Guibé, J. and Lamotte, M. La réserve naturelle intégrale du Mont Nimba. XXVIII. Batraciens du
genre Phrynobatrachus. Mem. Inst. fond. Afr. noire, 1963, 66, 601–627.
3. Lamotte, M. Les batraciens de la région de Gpakobo (Côte d’Ivoire). Bull. Inst. fond. Afr. noire,
1967, 29, 218–294.
4. Rödel, M.-O. and Ernst, R. A new reproductive mode for the genus Phrynobatrachus Günther,
1862: Phrynobatrachus alticola Guibé and Lamotte, 1961 has non-feeding, non-hatching tadpoles.
J. Herpetol., in press.
5. Rödel, M.-O., Range, F., Seppänen, J.-T. and Noë, R. Caviar in the rain forest: monkeys as frog
spawn predators in Taï National Park, Ivory Coast. J. Trop. Ecol., in press.
6. Rödel, M.-O. and Ernst, R. A new Phrynobatrachus species from the Upper Guinean rain forest,
West Africa, including the description of a new reproductive mode for the genus. J. Herpetol., in
press.
7. Rödel, M.-O., Krätz, D. and Ernst, R. The tadpole of Ptychadena aequiplicata (Werner, 1898) with
the description of a new reproductive mode for the genus (Amphibia: Anura: Ranidae). Alytes, in
press.
8. Schiøtz, A. The treefrogs (Rhacophoridae) of West Africa. Spolia zool. Mus. haun., 1967, 25, 1–
346.
143
Project ID: 01LC0017 (BIOTA West Africa W09)
1.1.2001-31.12.2003
THE INFLUENCE OF LOCAL AND REGIONAL FACTORS ON THE DIVERSITY,
STRUCTURE, AND FUNCTION OF WEST AFRICAN BAT COMMUNITIES
(CHIROPTERA)
Jakob Fahr, Njikoha M. Ebigbo and Elisabeth K.V. Kalko
Abt. Experimentelle Ökologie (Bio 3), Universität Ulm, Albert-Einstein Allee 11, 89069 Ulm
jakob.fahr@biologie.uni-ulm.de; http://www.biologie.uni-ulm.de/bio3
Key words: local assemblages, community ecology, guild structure, habitat heterogeneity, scale
dependency, distribution patterns, regional species pools, GIS, modeling, potential distribution,
frugivory, seed dispersal, plant-animal interactions, vegetation dynamics
Abstract:
Using a comprehensive approach, we examine the functional significance of bats for the
maintenance of biological diversity in Africa. The project goals are to determine the roles of bats
in fundamental ecological processes such as seed dispersal and to assess factors that influence
the structure and diversity of chiropteran assemblages on local and regional scales. In a first
approach we determine the composition and guild structure of selected bat communities on a
local scale (Ivory Coast) by conducting comparative field studies with standardised methodology
at two localities contrasting in vegetation composition (savanna and rain forest). In a modelling
approach, we analyse the large-scale, regional diversity patterns of West African bats based on
data of species distribution as well as biotic and abiotic habitat variables. We study the spatial
patterns of bat diversity with the ultimate goal to test underlying factors such as habitat
heterogeneity and productivity that might covary with these patterns. In a third approach we
examine the role of flying foxes (Megachiroptera) as keystone species in plant-animal interactions
and their influence on the spatial and seasonal vegetation patterns within the highly dynamic
forest-savanna mosaics of the Guinea zone.
Results:
In our first working package we have established a standardized protocol to sample local bat
assemblages. We ask how individual species and entire communities are associated with different
habitat types. Until now we have obtained long-term data for two local assemblages, one in the
rain forest zone (Taï-N.P.), the other in the Guinea zone (Comoé-N.P.), both of which are fairly
undisturbed. The study areas represent important examples of West African biomes that are
increasingly threatened by degradation. At present the data set for Taï-N.P. covers 2 years
(1999/2000) while the one for Comoé-N.P. was initiated in 1993/95 and now embraces 3 years
(1999-2001) of intense sampling, including the Biodiversity Observatory. The preliminary results
suggest that the habitat preferences of bats are generally very species-specific, thus making them
well-suited candidates to evaluate and monitor the effects of habitat alteration through land
conversion and global change. Furthermore, the structure of the assemblages (species and guild
composition, abundance patterns) reflect the different habitat types that have been sampled. These
data will serve as a base-line against which the effect and magnitude of land alterations can be
tested in the main phase of BIOLOG. So far, a total of 53 species which represent 84% of the
estimated 63±4 species (calculated with EstimateS, © R. Colwell) have been found in ComoéN.P. In Taï-N.P. we have recorded 37 species, representing 80% of the expected 46±2 species.
These preliminary data support our initial assumption (see BIOTA application) that the diversity
peak of bats in West Africa is not found in the rain forest zone but in the adjacent forest-savanna
144
mosaic of the Guinea zone. This diversity peak is caused by the exceptional habitat richness
which fosters an influx of species from habitats adjacent to the ecotone. The species richness of
Comoé-N.P. by far surpasses all other African bat communities that have been studied in the past.
It underlines our general hypothesis [1] that African bat communities are not depauperate as
proposed by FINDLEY [2] but attain levels comparable to the Neotropics. We conclude that habitat
degradation – especially in highly dynamic mosaic systems – is likely to negatively affect species
richness, composition and community pattern, potentially altering the function of dependent
ecosystem levels. This latter question will be addressed in the main phase of BIOLOG.
While our first working package focuses mainly on the local factors determining bat assemblages,
we investigate in our second working package the spatial patterns of bat diversity on a regional /
subcontinental scale and its interdependency with the local scale. For this approach we model the
habitat preferences and potential distribution patterns of all bat species occurring in West Africa.
To build species-specific habitat models, the known occurrences of each species (verified point
localities only) are superimposed onto biotic and abiotic variables (vegetation, topography,
climate) already implemented in the GIS of BIOTA-subproject W01. By superimposing the
modelled distribution patterns we identify the regional diversity patterns and regions with
exceptionally high species richness and / or endemism (hot spots). We have already established a
data base which comprises all known point localities of West African bat species (121) that have
been published (ca. 470 publications) and where specimens (about 20.000) are available in
museum collections in Europe and North America. It is the most comprehensive data set for any
group of African vertebrates. The modeling approach will allow us to overcome many of the
sampling biases by building species-specific habitat models and extrapolating them into space.
For this working package we are collaborating closely with BIOTA-subprojects W01 and W03.
Finally, in our third working package we study the role of flying foxes on the vegetation dynamics
of the Guinea savanna by determining their influence as seed dispersers. We investigate their
importance in detail by focusing on the tree species Cola cordifolia (Sterculiaceae) and Milicia
excelsa (Moraceae). First results indicate that both species are dominant elements of island forests
but with varying densities and/or patchy distribution within these forests. This pattern might be
caused by the activities of highly mobile seed dispersal agents. We therefore test the assumption
that flying foxes significantly influence seed distribution, having a high potential for long distance
dispersal. Direct observations at three fruiting tree species (C. cordifolia, Syzigium guineense and
Uapaca togoensis) suggest that flying foxes mainly feed by shuttling between resources and
feeding roosts. In an island forest, dominated by C. cordifolia, a high number of feeding roosts
(46) with seeds of this species could be identified within a small area of 2 km². The distribution of
feeding roosts appears to be concentrated in areas of least fruiting density. Although initial seed
deposition is high, germination rate appears to be moderate yet yielding a high density of
seedlings per area. Surprisingly, very few older age classes could be found so far. In close
cooperation with BIOTA-subproject W04 we have established ten 100 m² plots in which
seedlings will be individually marked and monitored over the next years to determine seedling
establishement, survival, and the resulting spatial pattern. In addition, we measure seed rain as
well as seedling density along transects oriented away from parent trees to assess distance effects.
1 FAHR, J., 1996. Die Chiroptera der Elfenbeinküste (unter Berücksichtigung des westafrikanischen
Raumes): Taxonomie, Habitatpräferenzen und Lebensgemeinschaften. Diplomarbeit, JuliusMaximilians-Universität Würzburg. 204 pp., 36 Karten.
2 FINDLEY, J. S., 1993. Bats - a Community Perspective. Cambridge University Press, Cambridge.
145
Project ID: 01LC0017 (BIOTA West Africa W11)
1.1.2001-31.12.2003
PHYTODIVERSITY IN THE WEST AFRICAN SAHEL AND SUDAN ZONE –
DEVELOPMENT AND EVALUATION
A. Akoegninou5 , W. Andres³, T. Bierschenk 4 , S. Dressler², K. Hahn-Hadjali, K. König³,
K. Neumann³, N. Schareika4 , M. Schmidt², B. Sinsin7 , A. Thiombiano 6 , R. Wittig, G.
Zizka².
1
Botanisches Institut, Universität Frankfurt. Email: hahn-hadjali@em.uni-frankfurt.de
² Botanik/Paläobotanik, Universität Frankfurt und Forschungsinstitut Senckenberg,
³ Institut für Physische Geographie, Universität Frankfurt
4
Institut für Ethnologie und Afrika-Studien, Universität Mainz
5
Faculté des Sciences et Techniques, Université Nationale du Bénin,
6
Unité de Formation et de Recherche en Sciences de la Vie et de la Terre, Université
Ouagadougou
7
Faculté des Sciences Agronomiques, Université Nationale du Bénin, Bénin
Key words: Phytodiversity, land use and changes in phytodiversity, ethnobotany,
archaeobotany, Poaceae, digitalization of herbarium collections.
Abstract:
In the Sahel and Sudan zone of Burkina Faso and north Benin,
biodiversity observatories according to BIOTA-standards,
based on satellite images and on observations in the field have
been selected and established (Fig. 1). For each of the 3
principal regions (representing Sahel, North and South
Sudanian zone), a pair of plots was selected, differing
principally in the intensity of land use, especially cattle
grazing. A floristic inventory as well as relevés of the subplots
were realized during field work. The ethnobotanical studies
concentrated on the perception/observation of changes in
flora and vegetation by local people and knowledge about
relevant species. The field work was performed in close co= Study sites
operation with the local counterparts (see above). The Fig. 1.
required contracts with the relevant local institutions and
counterparts where signed, serving also as research permits. The program BRAHMS was
installed on a new computer (financed by the project) in the national herbarium at University
Ouagadougou, BF. Digitalizing of collections was started and further training of staff is to
take place at the end of 2001.
Gorom-Gorom
Mali
Niger
Burkina Faso
Ouagadougou
Fada
N'Gourma
Bobo-Dioulasso
Natitingou
Benin
Togo
Elfenbeinküste
Nigeria
Cotonou
Ghana
0
250
500 km
Results:
As the field campaign will only finish in the beginning of November and the diversity data
acquired (inventories, reléves etc.) have not been analyzed yet in detail, the following results
can only be very preliminary and fragmentary. BIOTA W11 is an interdisciplinary research
project. Its objective is the analysis and evaluation of recent and historical changes in plant
diversity following a climatic gradient and varying land-uses. Better foundations for
sustainable utilization concepts and protection measures will be developed. A further
objective is the extension and cross-linking of West African plant collections to make the
analysis of collection data more efficient. The project is to be executed in co-operation with
counterparts at the Universities of Ouagadougou/Burkina Faso and Cotonou/Bénin and with
the SFB (collaborative research project) 268 „Westafrican Savanna“ of the University
Frankfurt/Main.
146
Especially the contacts, installations and scientific results of the SFB 268 and the excellent
co-operation with the counterparts were of great help to deal effectively with the authorities as
well as to locate and design the study plots for the BIOTA project.
For the selection of the plots, besides studies in the field satellite images proved to be highly
informative and helpful. For each of the 3 principal regions (representing Sahel, North and
South Sudanian zone), a pair of biodiversity observatories were selected. Floristic inventories
of vascular plants and relevés of subplots were realized. In co-operation with the
ethnobotanists, the kind, extent and duration of human impact on the study areas were
determined. The plots of each pair differ principally in the intensity of cattle grazing. Of
special interest is the fact, that we were authorized to establish one observatory in a reserve
strictly protected from grazing and agriculture since about 50 years. Designation and
investigation of plots took place in close co-operation with the scientific counterparts (see
above). PhD students from Burkina Faso and Benin are actively involved in the project and
partly also participated in the first field campaign.
The increasing intensity of grazing and agriculture have led to changes in the flora and
vegetation also realized by the local people [1], [2]. As expected, alpha-diversity alone cannot
serve to describe the changes: While species diversity seems to be lower in the least disturbed
habitats than in the moderately disturbed ones, the changes are considerable as far as the grass
layer is concerned. Besides the overall phytodiversity studies in the plots, special topics are
the diversity changes in selected groups assumed to be of special indicator value. While the
counterparts will in part concentrate on the woody plants, we will in the first period pay
special attention to the Poaceae. Height and structure of grass layer as well as species
composition vary considerably according to type and intensity of land use. Striking is the
decrease of abundance and diversity of perennial Poaceae and increase of annual grasses with
increasing grazing pressure [3], [4]. Dynamics in the distribution area of these indicator
groups are to be detected by the investigation of (especially historical) herbarium collections.
As part of this analysis, relevant material of the selected indicator groups in the herbarium
collections in Berlin (B) and Brussels (MEISE) were digitalized, and the entering of these
data in a BRAHMS database has begun. In order to facilitate the access to and exchange of
diversity data, the national herbarium at University Ouagadougou was provided with a new
computer which was installed together with a version of the program BRAHMS. This is to
serve the digitalizing of the herbarium collections to facilitate access to and exchange of
phytodiversity information. The same program has already been established in the course of a
Dutch project in the national herbarium of Benin in Cotonou. The training of the staff in
BRAHMS has commenced in October and will be continued in December 2001.
For the study of historical changes of the Sahelian phytodiversity, samples of charred wood
from the Sahel of Burkina Faso were identified, recovered from 20th century archaeological
sites. These include a large number of species, some of which have become rare in the
modern flora [5]. Comparison with the modern woody vegetation is done at present.
References
1. Hahn-Hadjali, K. & Thiombiano, A. (2001): Perception traditionelle des espèces en voie de disparition en
milieu gourmantché: causes et solution.- Ber. Sonderforschungsbericht 268, Bd. 14: 285-298.
2. Schmid, S., Braun-Yao, M., Hahn-Hadjali, K. & Werthmann, K. (2001): 10 ans plus tard: La situation socioéconomique et écologique dans l´ancien projet d´installation de migrants la province de la Bougouriba (Burkina
Faso).- Ber. Sonderforschungsbericht 268, Bd. 14: 535-542.
3. Wittig, R., Hahn-Hadjali, K. & Thiombiano, A. (2000): Besonderheiten von Flora und Vegetation der Chaîne
de Gobnangou im Südosten Burkina Fasos.- Etudes flor. veg. Burkina Faso 5: 49-64.
4. Wittig, R., Hahn-Hadjali, K., Krohmer, J. & Müller, J. (2000): Nutzung, Degradation und Regeneration von
Flora und Vegetation in westafrikanischen Savannenlandschaften.- Ber. R.-Tüxen Ges. 12: 263-281.
5. Neumann, K. (1999): Charcoal from West African savanna sites: questions of identification and interpretation.
In: van der Veen, M. (ed.): The Exploitation of Plant Resources in Ancient Africa. New York 1999 (Kluwer /
Plenum Publishers): 205-220.
147
Project ID: 01LC0022
1.1.2001 - 31.12.2003
THE CUVETTE CENTRALE AS RESERVOIR OF MEDICINAL PLANTS
Barbara Fruth
Max-Planck-Institut für Verhaltensphysiologie, Wicklerhaus 7, D-82319 Seewiesen
fruth@mpi-seewiesen.mpg.de; http://www.mpi-seewiesen.mpg.de/~knauer/fruth/fruth.html
Key words: flora, Congo basin, Cuvette Centrale, medicinal plants, ethnobotany
Abstract:
We wish to conduct a long-term project on plant diversity in the central region of the Congo
Basin, the Cuvette Centrale, Democratic Republic of Congo. During the initial phase (3
years) we seek to establish the personnel and infrastructural foundations. This implies the
project-oriented education and training of technicians and young academics at the University
of Kinshasa as well as the collection and determination of the flora of this area with specific
focus on natural medicinal plants. In order to characterise the study area in terms of it's plant
diversity and abundance, parameters such as measures of size (dominance), numbers
(density) and distribution (frequency) will be investigated. In addition, the historical and
current significance of natural herbal plants for the medication of both humans and animals
and their phytochemical characteristics will be documented. High priority will be given to the
evaluation and projection of possibilities of economic use of natural herbal plants within and
by the country of origin.
Results:
In the initial proposal, data collection was planned to be conducted at Basankusu (see figure),
Province of Equateur. This area was occupied by rebels opposing the official government
based in Kinshasa and thus became inaccessible for scientific research. Therefore, we
explored other areas within the central Congo basin and decided to establish a new site for
field research situated at the south-western border of Salonga National Park (see figure),
Province of Bandundu. The Institut Congolais pour la Conservation de la Nature (ICCN) has
been requested to grant permission to conduct field research within Salonga National Park and
the contract is now ready to be signed by the Minister of Environment.
During a pilot study in spring 2001, 15 transects (200 x 10m) were established for habitat
characteristics using methods established during earlier studies (Boubli et al. submitted).
Along these transects, 170 specimens of wild plants were collected. Reference copies of this
collection were stored in the herbaria of Kinshasa and Brussels University for identification.
Out of these, 42 species are already identified. About 50% of these plants are reported to be
used as medicinal plants by the local population (Bakundu).
Interdisciplinary cooperation with leading officials of Kinshasa University, environmental
organisations and NGOs was established. By the end of 2001 a small crew of graduates will
be trained and ready to start data collection in the upcoming year. A systematic investigation
of both quantitative and qualitative data in the field will put light on the floristic diversity of
the area and filter out plants of potential pharmacological interest. Goal is to obtain base-line
148
information for long term data collection and to present a comprehensive documentation of
the flora in form of an archive of plant taxonomy accessible to the public that will be backed
up by specimens in both Congolese and European herbaria. The historical and current
significance of the recorded medicinal plants for both humans and animals will be
investigated and presented as monographs.
References:
1. Boubli J.P., Eriksson J., Wich S., Hohmann G. & B. Fruth. submitted. Forest Habitat
Characteristics in the Lomako-Yekokora Interfluvium, Democratic Republic of the Congo.
149
Project ID: 01LC0025 (BIOTA East Africa E01)
1.04.2001-31.03.2004
BIOTA EAST AFRICA:
BIODIVERSITY IN CONVERSION – THE INFLUENCE OF FRAGMENTATION
AND MAN-MADE EXPLOITATION ON THE BIODIVERSITY OF TROPICAL
MONTANE FORESTS
C. M. Naumann, J. Köhler
Zoologisches Forschungsinstitut und Museum Alexander Koenig, Adenauerallee 160, 53113
Bonn, Germany
c.naumann.zfmk@uni-bonn.de, j.koehler.zfmk@uni-bonn.de, www.biota-africa.org, ww.museumkoenig.de
Introduction
BIOTA East Africa links a set of thematically and geographically strictly coordinated
analyses of biodiversity conversion in the East African highland and montane rain forests.
Analyses of biodiversity and its change are carried out mainly at Mt. Kenya and Kakamega
forest (Fig. 1), Kenya. The latter represents the eastern-most branch of the Guineo-Congolian
rain forest block, situated at 1400 to 1700 m a.s.l. in western Kenya. The area has protected
status as National Forest Reserve and includes both primary and secondary rain forest, as well
as several isolated fragments. It is perfectly suited to conduct comparative analyses of
biodiversity within the mentioned habitat types. The efforts are centred on the comparison of
primary and secondary highland rain forests with small isolated fragments. The developing
open area habitats (farm land, grazing areas, waste lands etc.) and the communities forming
within them are included. Comparative studies are carried out in other forest regions in
Kenya, Tanzania and Uganda.
In addition, five representative palaeo-african refugial areas have been selected to investigate
plant diversity and structure of xero-tropical montane communities in southern Yemen and on
Soqotra. obtained ecological data will help to estimate the present state of desertification and
to develop management plans for the preservation and sustainable use of unique xero-tropical
communities.
Scientific goals of BIOTA East Africa
Principal goal is the establishment of biodiversity observatories for long term monitoring,
with main focus on the effects of man-made changes in biodiversity. To achieve this aim,
significant interfaces of the trophic network of the tropical rain forest and its replacement
communities have been critically selected: plant-pollinator systems, forest fragmentation and
seed dispersal, exchange of atmospheric compounds, regeneration of tree species, decline in
anurans, diversity of coprophagous beetles, dragonflies, and Lepidoptera. The use of modern
remote sensing techniques and the collection of local and historical information on
environmental change will be used to establish a local information system in the study areas.
Experiments are carried out using standardised plots and sampling methods. The potential of
forest and savannah ecosystems for sustainable land use are investigated. As a result, we
expect a better understanding of the complex consequences of evolutionary change on the
different hierarchical levels resulting from degradation and fragmentation, especially at the
level of critical trophic and reproductive interfaces. In addition, we expect to be able to
provide useful tools and methods for rapid assessment procedures for selected systems.
150
Steps of the first months
The BIOTA East Africa project started in April 2001 with preparations for the Kick-Off-Trip
to Kenya which took place in May and June 2001. On this trip, all necessary formalities to
receive working and research permissions from Kenyan governmental officials were arranged
and numerous scientific cooperations with local and international institutions were established
through signing bilateral Memorandums of Understanding (MoU) and contracts. At the same
time, we took the first steps towards an establishment and improvement of research logistics
(field equipment, vehicles, research station, weather station, field assistants) and identified
certain areas to which the BIOTA East Africa research design should be applied. As a result
of these activities, all involved subprojects already conducted their first campaign of field
research and data sampling (mainly at Kakamega Forest and Mount Kenya).
Fig 1: satellite image of western Kenya, including the Nandi
escarpment and Kakamega Forest, the main study site of
BIOTA East Africa.
Established Cooperations
Beside the network of internal BIOTA cooperations, BIOTA East Africa established the
following cooperations with local and international counterparts (e.g. exchange of data, joint
education and training of students/para-taxonomists):
•
•
•
•
•
•
•
•
•
National Museums of Kenya (NMK), Nairobi
Kenya Wildlife Service (KWS), Nairobi
International Centre for Insect Physiology and Ecology (ICIPE), Nairobi
International Centre for Research in Agroforestry (ICRAF), Nairobi
Maseno University, Maseno
Kenyatta University, Nairobi
Makerere University, Kampala
EAFRINET (East African LOOP of BioNET International)
African Pollinator Initiative (API)
In addition, internal communication with BIOLOG biodiversity informatics projects (e.g.
EDIS, DORSA) revealed the necessity of linking voucher collections obtained through
BIOTA research with a sustainable data management for efficient use and long-term
accessibility of information.
151
Project ID: 01LC0025 (BIOTA East Africa E02)
1.4.2001 – 31.3.2004
IMPACT OF BIODIVERSITY CHANGE ON THE EXCHANGE POTENTIAL OF
TRACE GASES BETWEEN ECOSYSTEMS AND ATMOSPHERE
A. Awiti3 , G. Schaab1,2, M. Walsh3 , G. Strunz2 , R. Steinbrecher1
1) Fraunhofer-Institut für Atmosphärische Umweltforschung (IFU), Kreuzeckbahnstr. 19, 82467
Garmisch-Partenkirchen, Germany
2) Deutsches Zentrum für Luft- und Raumfahrt (DLR), 82234 Wessling, Germany
3) International Centre for Research in Agroforestry (ICRAF), Kisumu, Kenya
steinbrecher@ifu.fhg.de; http://www.ifu.fhg.de
Key words: surface fluxes, atmosphere, trace gases, VOC, agroforests, GIS, remote sensing, geospatial data
Abstract:
Tropical ecosystems produce a variety of trace gases playing decisive roles in the atmosphere by
triggering the oxidation power as well as the warming potential. Despite their relevance for the
regional and global atmosphere a sound understanding of the effect of tropical agroforestecosystems, showing different grades of plant diversity, on trace gas emission is lacking. To close
this gap, interdisciplinary research enterprise is necessary by coupling plant and soil
documentation with a satellite imagery-based geographic information system supplemented by
ecophysiological studies related to the production of the trace gases and canopy modelling. New
airborne surface flux measuring platforms will be developed and used to investigate the air
surface exchange of trace gases of ecosystems of different biocomplexity. For up-scaling from the
plot scale to regional scale ground as well as airborne measurements will be used to develop a
trace-gas-surface-flux model for the different land use types. The proposed activities will further
serve to document the effect of anthropogenic activities on the biodiversity of tropical forest
ecosystems as well as on the chemical composition of the tropical atmosphere, taking the
Kakamega Forest area and its change over the last thirty years as example.
Results:
(1) General tasks
• For an effective project performance, cooperations with institutions in Kenya (ICRAF,
ICIPE, ILRI, NMK, DRSRS, LPR) plus setting-up of geo-spatial data sharing agreements
(with ICIPE, ICRAF) supplementing memoranda of understanding were arranged. The
contract negotiation procedures with the BMBF, DLR and ICRAF have been finalised.
• During a first visit o the test area possible experimental plots were identified by overflying
Kakamega Forest and the surrounding.
(2) Development of an airborne surface flux measurement platform
• For airborne surface flux measurements a platform with a 5-holes-probe coupled with a
relaxed eddy accumulation device recently developed and quality assured is under
construction.
(3) Experiments at the Kakamega Forest site
• A replicated spatial analogue survey designed to assess the impacts of forest conversion
on soil productivity (greenhouse bioassay), carbon and macronutrient (C, N, P, K, S,
152
•
•
CEC), stocks soil physical properties (bulk density, water stable aggregates, texture) has
been completed. One hundred geographically paired sites contrasting primary/secondary
forest with subsistence maize agriculture were surveyed across the Kakamega and South
Nandi forest ecotones in Jul-Aug. 2001. All sites were georeferenced. Diffuse reflectance
measurements (350-2500 nm range) and physicochemical analyses of topsoil samples are
underway. A selected number of samples will also be analyzed for 210 Pb (210-Lead) and
137
Ce (137-Cesium) inventories, to contrast soil erosion-deposition rates between
agricultural and forest sites.
A protocol for rapid assessment of soil productivity was tested in Kisumu. Topsoil
samples were screened in a pot study using maize as an indicator species. The spectral and
physicochemical analysis of plant and soil samples is still underway.
A meteorological station has been set up in the Kakamega forest and is operating since mid of
September.
(4) Setting-up of the BIOTA-East-GIS
• First GIS-application in order to support the BIOTA-East kick-off trip to the Kakamega
Forest: combining topographic maps (1:50'000 scale), a vegetation map compiled by M.
Haupt, and photographs taken from an aircraft during the IFU kick-off trip
• Intensive search for available geo-spatial data by contacting different Kenyan, Swiss and
German institutions as well as for relevant literature, resulting in orders of relevant maps and
literature.
• Setting-up the BIOTA-East-GIS-structure considering different levels of detail: a) Africa, b)
Kenya, c) Kakamega Forest and associated forest areas, d) Kakamega Forest, e) observation
plots
• Integration of ready-available geo-spatial data sets of African or Kenyan extent from DCW
(PSU), DLR (Oberpfaffenhofen), ESRI (Redlands), FAO (Rome), ILRI (Nairobi),
UNEP/GRID (Geneva), UNEP/GRID (Nairobi), as well as of topographic maps covering the
Kakamega Forest and associated forest areas
• Ordering fine-resolution Landsat satellite imagery time series: 2001, 1995, 1989, 1984,
1979/80, 1975, 1972/73
• Presenting the BIOTA-East subproject E02 at international conferences (see references)
• Preparation and presentation of a seminar “GIS in Biological Sciences” at the NMK (Nairobi)
as a contribution to capacity building.
References:
1. Schaab, G., Steinbrecher, R., Forkel, R., Junkermann, W., Strunz, G., and Walsh, M., Der
Einfluß sich ändernder Biodiversität tropischer Waldökosysteme in Kenia auf den Austausch
ökologisch relevanter Spurenstoffe zwischen Ökosystemen und Atmosphäre – Aufgaben und
Einsatz von GIS im BIOTA-Ost-Teilprojekt E02. Poster at the Agit 2001, 4-6 July 2001,
Salzburg.
2. Schaab, G., and Steinbrecher, R., Land Cover Changes and its Impact on Biogenic VOC
Emission. Poster at the Global Change Open Science Conference, 10-13 July 2001,
Amsterdam (Abstract: Challenges of a Changing Earth, IGBP Stockholm ,P.6.0.015, 311,
2001).
3. Schaab, G., and Bernt, N., Ein GIS für Biodiversitätsforschung im Kakamega Forest
(Westkenia). DFD-internal Poster.
4. Schaab, G., Comparative analyses of biodiversity in the Kakamega Forest (Western Kenya) –
The use of GIS within the BIOTA-East project. Proceedings of the AfricaGIS Conference, 59 November 2001, Nairobi.
153
Project ID: 01LC0025 (BIOTA East Africa E03)
1.3.2001 – 31.3.2004
REGENERATION OF TROPICAL MONTANE TREE SPECIES – SPATIAL AND
TEMPORAL DYNAMICS OF FEED BACK PROCESSES
H. Dalitz, D. Uster, L. Straßl, W. Musila
Department of Ecology, Faculty of Biology, University of Bielefeld, Universitätsstraße 25, 33501
Bielefeld, Germany
Helmut.Dalitz@biologie.uni-bielefeld.de, www.biota-africa.org
Key words: Dispersion, diversity and composition of tree species; spatial variation of canopy
throughfall, nutrient elements and soil characteristics; regeneration of tree species; light climate;
growth;
Abstract:
The plot-based study is embedded into a research network which investigates different levels of
disturbance/fragmentation on processes which may influence changes in biodiversity. This study
focuses on processes which regulate the regeneration of tree species. Abiotic processes like water
and nutrient input via canopy thorughfall, biotic processes like growth of trees are intercorrelated
with changes in canopy structure, light climate and soil properties. All these processes controll
the regeneration of trees. Necessary cooperations with other research groups investigating
dissemination, diversity of tree species on larger scales give BIOTA the change to analyse spatial
and temporal changes. Close cooperations with Kenyan institutes helps to reach the aim of
observatorial areas in East Kenya.
Results:
Aims of this project can be divided into four main aspects:
1. Detailled description of tree species composition, dispersion and diversity in different sites of
the Kakamega forest in Kenya. This includes measurements of the incremental growth of tree
species measured with dendrometers, which gives the chance to analyse the influence of climate
on growth of tree species over a long period of time
2. Analysis of the spatial and temporal variations of rain fall and canopy throughfall as one
example of an element flux which could influence the species composition of trees.
3. Analysis of spatial variations of pH, EC, total element content and water extractable element
fraction in soils. The aim is the determination of spatial variations of soil nutrients at horizontal
and vertical dimensions at different sites in the Kakamega forest.
4. Analysis of the regeneration of tree species within the forest in 1 squaremeter plots.
Parameters which are measured: Species composition, dispersion and diversity of tree species,
incremental growth of trees, diversity and dispersion of seedlings and saplings, analysis of canopy
throughfall and rain fall in high temporal resolution, analysis of canopy structure using
hemispherical photographs, analysis of soil samples; in all samples (rain water, soil samples) main
nutrient elements, pH, EC and selected trace elements are to be analysed.
All investigations in this forest are plot-based which gives us the possibility to analyse spatial
patterns. The research area is influenced by the rural population at varying levels of selective
logging, collection of fire woods, sampling of medicinal plants and fragmentation into smaller
forest compartments. This allows the analysis of processes along an desturbance gradient.
154
The main idea behind the project: Soil and forest in Kakamega have developed over a long
period but during the last ~100 years the forest is influenced by the population. Different
levels of influence may have caused different changes in vegetation, changes in soils. Spatial
variations in canopy throughfall, light climate and changes in soil nutrient availability may
have resulted in changes in vegetation. All this processes are interrelated and have feed back
effects on each other. Analysing processes in water and nutrient input via canopy throughfall
in dependence on the structure of the vegetation, the light climate, soil properties and tree
species regeneration can help understanding the underlying processes and can be used as
biodiversity observatorial sites of changes in climate.
State of affairs (first field trip from August to November):
Since project start we had fixed problems concerning research permits; initiated close
cooperations with the ICRAF soil
department (Dr. Alain Albrecht) and with
the Herbarium and Botanical department of
the National Museum of Kenya (Dr. J.
Mutanga).
Demarcation of 12 plots at four different
sites in Kakamega (GPS data available):
Colobus trail, Buyangu Hill, Salazar circuit,
Isecheno. Plot size 20 to 20 m (see sketsch).
At 25 points per plot at 7 depths soil
samples were collected (2100 samples).
Additionally 600 samples of leaf litter (two
samples per soil auger hole) were collected.
In all plots tree species are determined, tree
height measured, BHD determined and
dispersion measured. 198 tree now have
dendrometers which allows the analysis of
incremental growth.
In 6 Plots we have installed collectors for
canopy throughfall. At 12 dates we have
already collections of rain fall and canopy
throughfall (daily readings).
Illustration of main
aspects of the project
(ellipses), cooperations
with other BIOTAgroups (boxes) and other
cooperations (rounded
boxes). The project is
closley related to the
DFG-project group in
Ecaudor, which allows
comparisions
of
processes between Costa
Rica,
Ecuador
and
Kenya. In all three
countries we work with
similar project designs
in tropical (pre-)montane rain forests.
155
Project ID: 01LC0025 (BIOTA East Africa E04)
1.4.2001-31.3.2004
INFLUENCE OF NATURAL AND ANTHROPOGENIC FRAGMENTATION
ON THE VEGETATION IN MONTANE RAINFORESTS OF KAKAMEGA
AND MT. KENYA
A. Althof, D. Killmann, A. Solga, E. Fischer
Institut für Biologie, Universität Koblenz-Landau, Rheinau 1, 56075 Koblenz, Germany
efischer@uni-koblenz.de, www.biota-africa.org
Keywords: Kakamega Forest, Mt. Kenya, primary forest, secondary forest, vascular plants,
stem epiphytes, bryophytes, lichens, Kenya
Abstract:
In Kakamega Forest, 35 plots of vascular plants and 130 plots of epiphytic bryophytes and
lichens have been studied in primary and secondary forest types. The primary forest stands are
characterized by a tree layer of 25-35 m, a dense shrub layer with dominating Dracaena
fragrans and a low coverage of herb layer. The secondary forests show a tree layer of usually
less than 10 m and a dense herb layer. In primary forests, hygrophytic bryophytes are
dominant on stems, while lichens are represented mainly by crustose species. Secondary
forests are characterized by the abundance of desiccation tolerant bryophytes and foliose and
fruticose lichens.
Results:
First field investigation in the northern part of Kakamega Forest revealed that large parts of
the forest are degraded and primary forest stands are restricted to only a few sites. 35
phytosociological relevés of vascular plant vegetation have been studied at Buyangu Hill, near
the Colobus-Trail, along the Isiukhu-River, around the KWS-Camp-Site and in the northern
Kisere fragment. At Buyangu Hill and near Colobus-Trail, the forest shows rather few older
trees but many secondary forest species. Probably there has been selective logging in the past.
The upper tree layer reaches 25-30 m and the following primary forest tree species have been
observed, e.g. Prunus africana, Diospyros abyssinica, Chrysophyllum albidum, Markhamia
lutea and Aningeria altissima. A rather dense shrub layer consisting mainly of Dracaena
fragrans is however typical for primary forest. The herb layer generally shows only low
coverage. Dominant taxa are Pollia condensata, Dorstenia brownii and the gap species
Desmodium repandum. In the rather abundant gaps at Colobus-trail, additional species are
Brillantaisia madagascariensis, B. vogeliana, Oplismenus hirtellus and Renealmia engleri.
The Forest in the northern Kisere fragment seems to be much more undisturbed. Here
large trees of nearly 35-40 m including Manilkara butugi, Diospyros abyssinica, Prunus
africana could be recorded. Also a dense shrub layer of Dracaena fragrans and Acanthus
eminens together with a low cover of the herb layer are characteristics for primary forest.
Along the Isiukhu river typical riverine forest with dense herb layer, the abundance of gap
species and a high bryophyte cover are typical features.
The investigated relevés in the secondary forest are characterized by an open canopy
usually less than 10 m tall and a dense herb layer with a high coverage. Typical trees or
shrubs for this forest type are: Rapanea melanophloeos, Acacia abyssinica, Acanthus
pubescens, Polyscias fulva, Neoboutonia macrocalyx and Croton megalocarpus. In the herb
layer, Aframomum zambesiacum, Achyranthes aspera, Desmodium repandum and Pavonia
156
urens are abundant. Also several terrestrial orchids like Eulophia streptopetala, Habenaria
malacophylla and Disperis aphylla have been observed, which could not be recorded in
primary forest plots.
Parallel to the investigation of vascular plants 130 phytosociological plots of epiphytic
bryophytes and lichens in the lower stem parts have been studied in the same areas in
Kakamega Forest. At Buyangu Hill and Colobus-trail, epiphytic vegetation was dominated by
hygrophytic bryophytes (Hepatics like Plagiochila spp., different Lejeuneaceae, e.g.
Lopholejeunea spp., Lejeunea spp. and mosses like Porothamnium spp., Neckera spp.).
Lichens were represented mainly by crustose species (e.g. Lepraria ssp.). Similar
observations could be made in the Kisere fragment.
The more open habitats along the Isiukhu-River allow the occurrence of crustose and
foliose lichens as well (e.g. Sticta spp., Lobaria spp., Leptogium spp.). Among the bryophytes
pendant species like Pilotrichella ampullacea are dominating.
The secondary forests studied are characterized by a high diversity of foliose and
fruticose lichens (e.g. Parmotrema spp., Heterodermia spp., Usnea spp., Leptogium spp.).
The epiphytic bryophyte flora consists mainly of desiccation tolerant taxa like Frullania spp.,
Brachymenium spp. and Orthotrichum spp..
Diversity in epiphytic relevés is much higher in secondary forest than in primary
forest. In the latter usually not more than 10 species were observed in a 50 x 20 cm plot while
in secondary forest up to 25 species occurred.
Additionally 40 relevés of epiphytic bryophytes and lichens have been recorded on
Mt. Kenya. The study sites were situated in the montane Olea-Podocarpus forest at 2500 m
a.s.l., the bamboo-zone (Sinarundinaria alpina) at 2700 m and the Hagenia-zone at 3000 m.
In contrast to Kakamega Forest the more open montane forests are dominated by large Usnea
species. Generally natural disturbance by Buffalos (Syncerus caffer) and elephants
(Loxodonta africana) occurs in all forest types.
157
Project ID: 01LC0025 (BIOTA East Africa E06)
1.3.2001 – 31.03.2004
LEPIDOPTERA AS INDICATORS OF HUMAN IMPACT ON TROPICAL
RAINFOREST ECOSYSTEMS IN EASTERN AFRICA
L. Kühne 1 , Ch. L. Häuser2 , J. Holstein2
¹ Museum für Naturkunde, Humboldt-Universität, Invalidenstr. 43, D-10115 Berlin, Germany
² Staatliches Museum für Naturkunde, Rosenstein 1, D-70191 Stuttgart, Germany
LIBAQ-Potsdam@t-online.de, chaeuser@gmx.de, holstein.smns@naturkundemuseum-bw.de
Key words: biodiversity, rainforest, Kenya, butterflies, moths, Lepidoptera
Objectives:
Afrotropical rainforest habitats have changed rapidly due to anthropogenic influences over the
last centuries, and have become increasingly fragmented and disturbed with growing demands
on land use by the local population. The effects on insect biodiversity and, in particular,
Lepidoptera are poorly known, as basic faunistic data about such groups are still scarce [1,3].
Diversity and subsistence of phytophagous insects like Lepidoptera depend on various abiotic
and biotic parameters such as microclimate, soil-characteristics, vegetation composition and
structure, and availability of specific larval and adult food resources, which all are directly
influenced by the different forms of historic and current land use.
The project aims to describe, characterise and compare the alpha- and beta-diversity of
selected families of diurnal and nocturnal Lepidoptera in natural, semi-natural and disturbed
habitats in standardised BIOTA biodiversity observatories for the area of Kakamega Forest in
western Kenya. This approach should allow to identify suitable sets of indicator species for
effectively assessing the relative impact of different land use patterns on insect (Lepidoptera)
rainforest communities in the region. At a later stage, it is intended to expand the obtained
results by comparative studies planned at Côte d’Ivoire (Comoe N.P.) and in combination
with data from related BIOTA projects (E05, E07, E09, E10) to become applicable for
tropical rainforest ecosystems throughout eastern and western Africa.
For the starting phase of the project, the focus is to refine methodological standards for the
required field surveys, including techniques for recording, determination of taxa, and data
analysis. For the entire duration of the project, the following steps are considered:
• Inventorying the Lepidoptera diversity (8-10 families) for selected plots
• Characterisation of the Lepidoptera community for every selected plot, in relation to past
and present human impact
• Comparison of Lepidoptera communities between different habitats, in relation to abiotic
and biotic parameters and human disturbance factors
• Identification of indicator species for different types of forest habitats / land use impact
• Development of assessment schemes for evaluation of land use patterns on the current
state of insect biodiversity in (East) African forest ecosystems
For this project, it is necessary to rely on comparatively well known groups. The following
Lepidoptera families have already been identified as promising candidates in Eastern Africa:
Papilionidae, Pieridae, Nymphalidae, Saturniidae, Sphingidae, and Arctiidae [2]. In addition to
recording Lepidoptera, soil samples will be taken for all sample plots and jointly analysed
(BIOTA E02, E03) to recognise historic changes in land use patterns.
158
Methods (field surveys):
Standard protocols and techniques for recording and monitoring Lepidoptera diversity are
refined to be used in different forest habitats [4]. Intense fieldwork will be carried out at the
selected plots/observatories twice in a year during a period of 4-6 weeks, around the
beginning and the end of the rainy season, which will later on be supplemented by monitoring
activities throughout the year.
For recording diurnal Lepidoptera, transect walks will be conducted periodically (three times
a week) at definite times during daylight. All butterflies seen within the bounds of the trail
and within a distance of 5 m ahead of the observer are recorded. For recording fruit feeding
Nymphalidae, baited traps will be used additionally along the transect routes.
Nocturnal Lepidoptera are recorded by light using both standardised automatic (15 W) as well
as manual light traps (125 W) (figs. 1, 2). Recording occurs three times a in periods ranging
from 3 hours to the entire night. All species of the selected Lepidoptera families are recorded
quantitatively, and individual records will be stored in a central database for further statistic
and biometrical analysis of diversity.
Furthermore, small soil samples (100 ccm) taken from homogenous patches within the
observatories will be analysed for physical properties and chemical composition following
standard procedures.
Fig.1: automatic light trap
Fig.2: manual light trap ('tower')
References:
1. Fermon, H., Waltert, M., Larsen, T.B., Dall’Asta, U. & Mühlenberg, M. 2000: Effects of forest
management on diversity and abundance of fruit-feeding nymphalid butterflies in south-eastern Côte
d’Ivoire. - Journal of Insect Conservation, 4: 173–188.
2. Larsen, T.B. 1996: Butterflies as indicator species in Africa - Presidential address. - Tropical
Lepidoptera News, 3: 1–4.
3. Mey, W., Nuß, M. & Speidel, W. 1998: Data about Lepidoptera diversity in the Afromontane region of
Malawi (Insecta, Microlepidoptera and Heterocera). - Memoires de la Société Royale Belge
d'Entomologie, 38: 155-170.
4. Pollard, E. & Yates, T.J. 1995: Monitoring Butterflies for Ecology and Conservation. – Chapman &
Hall, London, 274 pp.
159
Project ID: 01LC0025 (BIOTA East Africa E07)
01.05.2001-30.04.2004
DIVERSITY AND SPECIES COMPOSITION OF ODONATA AS INDICATORS OF
BIOTOPE QUALITY OF EAST AFRICAN RAIN FORESTS AND THEIR
REPLACEMENT COMMUNITIES
V. Clausnitzer
Geographisches Institut, Universität Marburg, Deutschhausstr. 10, 35037 Marburg, Germany
wesche@mailer.uni-marburg.de, www.uni-marburg.de, www.biota-africa.org
Key words: dragonflies, Odonata, biodiversity, Kenya, Tanzania, Uganda, wetlands, coastal
forests
Abstract:
The principal aim of this project is a comparative study of ecology, diversity and biogeography of
dragonflies (Insecta: Odonata) in primary, secondary and fragmented rain forests and different
wetland habitats in East Africa. To achieve this an identification key for the dragonflies of
eastern Africa is prepared and distribution data as well as data on species specific habitat
requirements are collected. These will be used for further applied studies, e.g. the development of
indicator systems for environmental disturbances.
Results:
Utilising both, aquatic and terrestrial habitats, the large, predominantly diurnal dragonflies,
who can be readily observed and in most cases easily identified in the field, can contribute
much to the evaluation of environmental quality. They are known to be very sensitive to
structural habitat quality and thus can be a valuable tool to evaluate landscape degradation
and have been used as indicator species. The amphibious larvae of dragonflies are critical in
regard to water quality and aquatic morphology of streams. The adults are sensitive to habitat
structure and are excellent indicators of river disturbance, e.g. changes in habitat structure.
Within this project a throughout inventory of East African dragonflies in various habitats and
the collection of species specific ecological data is aimed at.
Current activities
Data collection has been started in different areas in Kenya, Uganda and Tanzania. In most
areas visited, new records for the could be made and a lot of data on various ecological
aspects could be collected. The PhD student John Joseph Kisakye, (Makerere University,
Kampala, funded through the BIOTA programme) works on dragonflies in different forests.
In cooperation with a GEF project in southern Tanzania it is planned to have Master's projects
on the impact of habitat degradation on dragonflies from 2002.
Coastal forests in Kenya
During the last year coastal forests of Kenya were surveyed to a large extend. Coastal forests
are listed as important areas in terms of conservation for East Africa [1] and are a major
centre of endemism in Africa [2, 3]. Coastal forest areas of Kenya and Tanzania are the
primary habitat for a number of highly localised dragonfly species (endemics and/or of unique
taxonomy). Detailed studies were carried out on the ecology of the two dendrolimnetic
species Coryphagrion grandis and Hadrothemis scabrifrons. Dragonfly communities relative
to different habitat types from indigenous forests to cultivated landscapes were described.
160
Most of the forest species are confined to coastal forests of East Africa, being stenotopic and
highly sensitive to disturbance. With increasing habitat disturbance eurytopic species which
are common and widely distributed in Africa colonise the habitats. The species assemblages
between different habitat types (stream, swamp, pool) in the disturbed landscape are identical,
the β-diversity being very low, although the diversity of single localities may increase after
habitat disturbances.
Publicity work
Species check-lists for several National Parks and other protected areas of Kenya have been
prepared and were presented to the corresponding authorities. Information boards on the local
dragonflies were prepared for some visitor centres (Saiwa Swamp NP, Mt. Elgon NP,
Arabuke Sokoke Forest, Nairobi NP). For 2002 a small dragonfly workshop is planned in the
Institute of Environmental and Natural Resources, Makerere University, Kampala.
Cooperations
Dragonflies are included in a Wildlife Conservation Society (WCS) project in Tanzania
"Southern Highlands Conservation Programme" and in a DANIDA funded project on IBA’s
in Uganda. Within all projects national reference collections are build up and local scientists
trained. First negotiations have started with Ethiopia to include dragonflies in a planned
inventory of insects. Together with BIOTA S08 species assemblages in arid habitats,
colonisation strategies in seasonal habitats and population genetics of species with a
panafrican distribution and different dispersal types are comparatively studied.
Odonatologists working in Africa are linked through the recently started PHAON (Pinhey's
Heritage African Odonata Network), which proves to be an excellent tool to exchange views
and data, discuss results and present projects. In cooperation with other scientists an atlas for
African Odonata and a mapping programme are long term aims.
Publications
The identification key of East African dragonflies will be published in 2002 or 2003. A
regional report on dragonflies of Eastern Africa for the IUCN Odonata Specialist Group has
been recently prepared. At least one new species waits for description, while a number of
other projects need more field work. Following recent manuscripts could be finished:
Clausnitzer, V. 2001a. Notes on the species diversity of East African Odonata, with a checklist of
species. Odonatologica 30:49-66
Clausnitzer, V. 2001b. Notes on Trithemis bifida and T. donaldsoni (Odonata: Libellulidae). The
International Journal of Odonatologogy 4:179-189
Clausnitzer, V. in press. Reproductive behaviour and ecology in the dendrolimnetic Hadrothemis
scabrifrons. The International Journal of Odonatologogy.
Clausnitzer, V., Lindeboom, M. submitted. Natural history and description of the dendrolimnetic
larvae of Coryphagrion grandis.
Clausnitzer, V. submitted. Dragonfly communities in coastal habitats of Kenya: indication of biotope
quality and the need of conservation measures.
References
1. Stuart, S. N., Adams, R.J., Jenkins, M. S. 1990. Biodiversity in sub-saharan Africa and its islands conservation, management and sustainable use. IUCN, Gland & Camebridge
2. Fjeldsa, J., and Lovett, J.C. 1997. Geographical patterns of old and young species in African forest
biota: the significance of specific montane areas as evolutionary centres. Biodiversity and
Conservation 6:325-346
3. Kingdon, J. 1989. Island Africa. Princetown University Press, Princetown
161
Project ID: 01LC0025 (BIOTA East Africa E08)
1.4.2001 – 31.3.2004
MEASURING AND MONITORING AMPHIBIAN DIVERSITY IN EAST AFRICA:
SEPARATING GLOBAL FROM REGIONAL AND LOCAL CAUSES
S. Lötters 1 , J. Kosuch1 , D. Rotich2 and M. Veith1
1
Institute of Zoology, University of Mainz, Saarstrasse 21, 55099 Mainz, Germany
National Museums of Kenya, Herpetology Department, P.O. Box 40658, Nairobi, Kenya
2
stefan@oekologie.biologie.uni-mainz.de; www.gadag.org; www.biota-africa.org
Key words: global amphibian decline, community ecology, global ecotypes, indicator species,
monitoring, population structure, molecular genetics, standardised methodology, Kenya
Abstract:
Global amphibian decline is currently a controversial issue. However, to judge upon a worldwide phenomenon needs world-wide monitoring. The Global Amphibian Diversity Analysis
Group (GADAG) is setting up a long-term monitoring of circum-equatorial anuran communities.
It focuses on ecologically similar species in comparable habitats. Under a GADAG framework,
BIOTA E08 studies amphibian communities in eastern Africa (Kenya) along a transect of isolated
rainforests of different degrees of fragmentation. This research is methodologically equivalent
with analogous projects in West-Africa (BIOTA W08) and Borneo and ally coherent with other
BIOTA projects in Kenya. Amphibian diversity is measured at three different levels: the
community, the species, and the population level. Species for monitoring are identified, different
field methods are tested and compared with other projects (e.g. BIOTA W08). A molecular
genetic database of African amphibians is being established and distribution data are
implemented in a BIOTA-compatible GIS.
Results:
For more than one decade now, scientists have noticed tremendous population declines in
amphibians all over the world. It is controversially discussed if these observations correspond to a
global biodiversity crises – or if local or regional causes are more responsible [1, 2].
The project BIOTA E08 has the intention to contribute to resolving this controversial. It is part of
a world-wide measuring and monitoring network for amphibian diversity at different levels,
incorporated in the Global Amphibian Diversity Analysis Group (GADAG). This group currently
operates in East and West Africa under BIOTA as well as in Borneo. Additional projects are
being added to run long-term observations of amphibian populations at the global scale after the
current three projects will have provided methodical standards. Only a world-wide diversity
analysis system dealing with similar methodology on comparable species (“ecotypes”) can
provide an answer to the question “global or local?” [3]. The BIOTA framework is especially
suitable for GADAG’s goals, because of the inter- and trans-disciplinary character of BIOLOG.
BIOTA E08 focuses on three different levels of amphibian diversity, the community, species and
population levels. The specific goals are
- to long-term monitoring of amphibian communities and relative abundance of species.
- to detect anuran species and populations to be monitored (indicator species)
- to define monitoring standards for amphibian populations.
- to analyse the diversity change of all occurring species and the demographic and genetic
development of indicator species with respect to environmental (human-caused) change.
Main study area of BIOTA E08 is the Kakamega Forest, added by four additional sites for
comparisons: Mount Elgon, Aberdares, Mount Kenya and Runda. We share the Kakamega Forest
162
with all BIOTA projects dealing with E Africa. Mount Kenya is also shared with BIOTA projects
E04 and E06. Study sites used by all three projects are already determined.
In 2001, all study sites have been surveyed for amphibians. Data from scientific collections (i.e.
National Museums of Kenya, Nairobi; California Academy of Sciences, San Francisco) were
added. We can already tentatively provide species lists for Kakamega Forest (21 spp.), Mount
Elgon (10), Aberdares (13), Mount Kenya (10) and Runda (15). The total number of species
found is 39. Relationships between the localities mentioned is shown in Fig. 1. Field and museum
data are currently made GIS-compatible.
Fig. 1: Comparison of five East African
amphibian communities, based on
presence-absence data.
Kakamega
Mt. Elgon
Aberdares
Runda
Mt. Kenya
3,0
3,5
4,0
4,5
5,0
5,5
Euclidian distance
Miscellaneous life history and syn-ecological data were recorded for most species (e.g. circadian
activity, vocalisations, reproductive modes, predators, prey, climatic preferences). These data are
important to taxa that are suitable as. We preliminarily identified potential indicator species for
long-term monitoring: Hyperolius viridiflavus, H. pantherinus, Ptychadena mascareniensis,
Phrynobatrachus natalensis, Xenopus borealis. They refer to amphibian ecotypes, currently being
defined for the entire tropics by GADAG [4].
Field methods for amphibians monitoring have been tested at Kakamega Forest, including
opportunistic, quadrate and transect sampling [5]. Field methods are being evaluated, and
extrapolation of species richness of communities is tested (cooperation with BIOTA W-08 and the
GADAG Borneo project).
Molecular methods are used in two ways:
- Molecular taxonomy: Unambiguously IDs of hardly determinable species, species
complexes and larvae through DNA sequencing. A molecular database has been
established. It presently harbours DNA data of 123 African amphibian taxa.
- Monitoring of genetic population characters: Genetic erosion starts prior to population
decline. Therefore, all occurring species are currently screened to identify taxa with high
genetic variability for later long-term observations.
A GIS for amphibian distributions is currently being set-up in cooperation with BIOTA E02 to
link former and current distributions of species with geo-spatial landscape information.
References:
1. Houlahan, J. E. et al. 2000. Nature 404, 752-755.
2. Ross, A.A. et al. 1999. Ann. Rev. Ecol. Syst. 30, 133-165.
3. Lötters, S. et al. Measuring and monitoring amphibian diversity change: separating global from
regional and local causes. Abstract, Joint Ann. Meet. Herp. League & Soc. Stud. Amphib. Rep.,
Indianapolis, 27-31 July 2001.
4. Veith et al. A global view of convergence from the frog perspective. In prep.
5. Heyer, W. R. et al. 1994. Smiths. Inst. Press.
163
Project ID: 01LC0025 (BIOTA East Africa E09)
01.03.2001 – 31.03.2004
THE INFLUENCE OF LAND USE MODES ON DIVERSITY, ABUNDANCE AND
GUILD STRUCTURE OF COPROPHAGOUS BEETLES IN THE AFRICAN
FOREST-SAVANNA-MOSAIC
F.-T. Krell1,2, D. Newman1,2,3 , S. Krell-Westerwalbesloh1,2, V. Mahiva4
1
Zoologisches Forschungsinstitut und Museum Alexander Koenig, Adenauerallee 160,
D-53113 Bonn, Germany
2
Department of Entomology, The Natural History Museum, Cromwell Road, London SW7 5BD,
U.K.
3
Department of Agriculture, The University of Reading, Earley Gate, P.O. Box 236, Reading
RG6 6AT, U.K.
4
International Centre of Insect Physiology and Ecology (ICIPE), Duduville, Kasarani, P.O. Box
30772, Nyayo Stadium, Nairobi, Kenya
E-mail: f.krell@nhm.ac.uk, www.biota-africa.org, , www.museum-koenig.de
Key words: dung beetles, pasture, agriculture, forestry, fire ecology, community ecology, species
diversity
Abstract:
Dung beetles (Scarabaeoidea) show their highest global diversity and abundance in African
forest-savanna mosaics. They are responsible for the largest portion of dung decomposition and,
thus, take part in the maintenance of soil fertility. The habitats of the forest-savanna mosaic are
subject to increasing man-made change and degradation. In this project we analyse changes of aand ß-diversity, abundance, and guild structure of coprophilous beetles caused by different modes
of human land use. Based on quantitative comparative data to be collected in Kenya and Côte
d’Ivoire we will analyse to which extent the most important influences, i.e. decrease of
autochthonous game fauna by poaching, traditional extensive cattle farming, forestry, agriculture
and bush fires, affect the species composition, guild structure and abundance of coprocenoses
and, consequently, soil fertility. In addition, we will investigate the historical reasons for the
origin and the maintenance of the high diversity of Afrotropical dung beetles to develop strategies
for the conservation of this unique, diverse fauna.
Results:
Dung beetles assemblages are an ideal model system for studying effects of human influences on
natural habitats because they respond to changes of vegetation structure and microclimate. During
the pilot period of the project (2001-2004), we are focusing on the effects of bush fires, poaching
and extensive cattle farming. Bush fires are traditionally lit to clear forest areas for agriculture and
plantations, and to burn savanna areas to improve extensive cattle farming and hunting.
We established study sites at the border between forest and savanna in the V-Baoulé in Côte
d’Ivoire (near the Bringakro research station of the Centre Suisse de Recherches Scientifiques),
and in the forest-savanna mosaic region (near the research station of the Universität Würzburg in
the Parc National de la Comoé). We sampled at a savanna site that is regularly used as cattle
pasture, a savanna site without cattle grazing (Imperata grassland), forest islands in the grazed
savanna, in a secondary forest, a coffee plantation (burned 17 years ago) and in a yams field in the
forest (freshly burned; Fig. 1). Plots of 20 m2 protected from grazing and protected from fire (Fig.
2), respectively, were installed in the grazed savanna of Bringakro. Additional unburned plots are
164
chosen in the Comoé Park to be established during the next dry season.
Fig. 2 : Firebreak around a 20 m2 -plot of
savanna parkland, regularly used as cattle
pasture; Bringakro, Côte d‘Ivoire.
Fig. 1 : Yams field (Dioscorea sp.) on a
recently burned secondary forest site;
Bringakro, Côte d’Ivoire.
The first samples indicate that abundance and diversity of dung beetles is lowest in the secondary
forest, higher in the open degraded areas in the secondary forest (coffee plantation and yams field)
but highest in the grazed savanna. However, compared with the Parc National de la Comoé, that
has still a rich autochthonous game fauna, the species number in the grazed savanna of Bringakro
seems to be lower. Baseline data on the dung beetle fauna of the Comoé Park are already
available [1, manuscripts submitted].
Our prospecting trip to Kenya is scheduled for October 2001. The sampling programme in
Kakamega forest (different forest types and degraded areas around) will start in 2002. Also in
2002, we will start sampling the third study area in Côte d’Ivoire, the Reserve d’Aboukouamekro
(a former cattle farm where native large mammals have been reintroduced). Comparing dung
beetle assemblages of a region with persistent autochthonous game, fauna with an area with
secondary introduced game and with a region where big mammals are extinct and have been
replaced by cattle herds, may indicate the relation between mammal fauna and dung beetle
assemblages. To evaluate the importance of coprophagous beetles for soil fertility, we started
measurements of the amount of buried dung for the species responsible for the highest percentage
of dung beetle biomass. Comparisons between dung beetle assemblages of geographically distant
regions of the Afrotropical forest-savanna mosaic (Kenya and Côte d’Ivoire) will indicate which
of the effects of human land-use on these organisms are general and which are determined by
regional parameters.
In addition, we will investigate the history of the present scarab fauna of East and West Africa
using phylogenetic and biogeographic analyses. This will elucidate how taxa and zoogeographical
elements react to man-made habitat changes. Comparing dung beetle faunas of regions with and
without species-rich autochthonous large mammal faunas we will draw conclusions on the
importance of the latter for developing the extraordinary diversity of dung beetles in African
forest-savanna mosaics. In the long term we will develop conservation strategies for maintaining
dung beetle diversity, as well as an indicator system for rapid evaluation and assessment of
biodiversity, and for estimating the degree of its endangerment.
References:
1. Krell, F. and Linsenmair, K.E. 1998. Blatthornkäfer einer westafrikanischen Savanne - Vielfalt in Kot
und Aas (Coleoptera: Scarabaeoidea). Bielefelder Ökologische Beiträge 12: 36.
165
Project ID: 01LC0025 (BIOTA East Africa E10)
1.4.2001 – 31.3.2004
EFFECTS OF HABITAT FRAGMENTATION ON
INTERACTIONS IN EAST AFRICAN RAINFORESTS
PLANT-POLLINATOR
M. Kraemer, Th. Bergsdorf
Alexander Koenig Research Institute and Museum of Zoology, University of Bonn,
Adenauerallee 160, 53113 Bonn, Germany
m.kraemer.zfmk@uni-bonn.de; thomas@bergsdorf.com; http://www.museum-koenig.de
Key words: pollination, plant-pollinator interactions, habitat fragmentation, Kenya
Abstract:
The project analyses the influence of forest fragmentation on plant-pollinator
interactions. First results indicate a significant difference in flower visitation rates
between different forest plots for the understory shrub Acanthopale pubescens.
However, a clearcut pattern between continuous forest and forest fragments cannot
be shown as yet.
Background:
It is widely recognized that fragmentation of natural habitats not only affects the
distribution and the abundance of organisms, but that it may disrupt the important
biological processes that maintain biodiversity and that are of high importance for the
functioning and the longterm existence of ecosystems. The pollination of plants by
animals represents such an integrative process - almost 100 percent of the flowering
plants in tropical rainforests are pollinated by animals. The main aim of this project
is to analyse the alterations of plant-pollinator interactions caused by habitat
fragmentation and the consequences for the long-term maintenance of tropical
biodiversity.
Cooperation:
The cooperation with the National Museums of Kenya and the International Centre of
Insect Physiology and Ecology, both Nairobi, were firmly established. As pollination
is a very important integrating process within and among natural communities,
ecosystems and agricultural systems, the project is highly appreciated by our
counterparts and Kenyan officials. Furthermore, a close connection to the African
Pollinator Initiative (C. Eardley, South Africa) was established, resulting in the
participation in the First Pan-African Pollination Workshop in February 2002.
Results:
The study is carried out at the Kakamega Forest Nature Reserve, which forms the
easternmost part of the afrotropical, guineo-congolian rainforests. During the first
field stay (June – October 2001) we established one study site in the northern part of
the forest, and another site in the southern part, each consisting of continuous forest
(Colobus, Salazar, Isecheno) and different forest fragments (Kisere and Malawa in
the north, Yala, Kaimosi and Ikuywa in the south). In order to tightly connect the
pollination project to the project on seed dispersal (E11) both groups use exactly the
same study plots. Due to logistic problems, forest fragments in the eastern part of the
nature reserve are to be excluded from the study.
166
Contrary to the preliminary studies in 2000, and due to the exceptionally high rainfalls
in early 2001, flowering in general was sparse and so was pollinator activity. So far,
our studies concentrated on Acanthopale pubescens (Acanthaceae), an understory
shrub that – with one exeption – occurs in all forest fragments. Acanthopale pubescens
flowers from July to August and is abundant enough for statistical purposes. Its showy
white flowers are quite attractive to a variety of insects and are most probably
pollinated by bees (e.g., naturally occuring honeybees). More than 150 individual
plants were selected for different purposes. In all forest plots we collected data of the
different levels of pollination (i.e., frequency of flower visits, pollination success
(pollen tube counting), fruit and seed production) and sampled possible pollinators by
standardized methods like sweep netting and malaise traps. Here, the help of Caleb
Analo is highly acknowledged. Comparative data on pollinator diversity is, however,
not yet available, as we began trapping insects regularly just in October 2001.
Based on about 300 one-hour
observation sessions we can
demonstrate significant differences
(one-way ANOVA) in flower
0,2
visitation rates between study sites
(Fig. 1). However, no pattern that
holds generally for differences
0,1
between fragments and continuous
forest can be shown so far. This is
certainly due to the fact that only a
single plant species was included
0,0
N = 46
35
54
27
41
34
22
in the study. The data on fruit and
Colobus Salazar Kisere
IsechenoYala Ikuywa Kaimosi
seed production that are presently
study site
analyzed
will
surely
reveal
0,3
important additional information.
Fig. 1. Insect visitation rate (mean ± s.e.) of flowers of
Acanthopale pubescens at different forest fragments and Furthermore, forest fragmentation
continuous forest (underlined) in the north (left) and the at the Kakamega site obviously is
south (right) of Kakamega Nature Reserve.
strongly overlayed by other
processes
like
present-day
anthropogenic disturbance, above all logging and grazing. The Kaimosi fragment, for
instance, although privately owned, is an extremely degraded forest where
Acanthopale pubescens is superabundant. On the contrary, the Kisere fragment with its
not too abundant A. pubescens, is one of the best maintained forest fragments of the
north. Among other reasons, at both sites, the high visitation rates may be directly
related to the plants abundance or, alternatively, may be the result of the lack of
alternative resources. To address these uncertainties it is necessary to incorporate other
plant species in the study. We already selected the abundant Dracaena fragrans,
Acanthus eminens and Coffea sp. for our further investigations that will supposedly
start in January 2002.
visits / flower / hour
0,3
167
Project ID: 01LC0025 (BIOTA East Africa E11)
1.1.2001 – 31.12.2004
EFFECT OF FRAGMENTATION ON SEED DISPERSAL, REGENERATION AND
DYNAMICS OF KAKAMEGA FOREST, KENYA
B. Bleher, K. Böhning-Gaese
Institut für Zoologie, Abteilung V, Johannes Gutenberg-Universität, Becherweg 13, 55099 Mainz,
Germany
b_bleher@web.de, www.biologie.oekologie.uni-mainz.de, www.biota-africa.org
Key words: biodiversity, ecosystem processes, fragmentation, frugivores, fruiting plants,
regeneration, seed dispersal, seedling recruitment
Abstract:
This project investigates the influence of forest fragmentation on biodiversity and ecosystem
processes related to seed dispersal and forest regeneration in Kakamega Forest. Data on the
diversity of frugivorous animals as potential seed dispersers and on the diversity of fruiting trees,
shrubs and lianes are currently gathered comparatively in four continuous forest and five forest
fragment plots. In a first inventory, seedling recruitment of primary forest tree species was
mapped at the same sites to obtain a first understanding of the influence of fragmentation on
forest regeneration. Seed dispersal, seedling recruitment and regeneration will now be studied in
depth in a few selected tree species (i.e. Prunus africana, Olea capensis, Antiaris toxicaria).
Results:
The greatest threats to the biodiversity of tropical forests are habitat loss and forest fragmentation.
Understanding how fragmentation affects ecological processes that involve animal and plant
populations and how this influences species and habitat persistence in the long term is particularly
important but hardly understood [1,2,3]. Seed dispersal by animals is one mutualistic interaction
which is essential for the long-term maintenance of populations of tree species in tropical forests.
Fragmentation disrupts animal populations and might lead to a decline in frugivore diversity in
forest fragments. Consequently, this might result in a break-down of dispersal processes
influencing the regenerative potential of forest ecosystems, leading to changes in abundance and
spatial distribution of plants [4,5] with far-reaching consequences for the diversity, dynamics and
stability of forest ecosystems [6,7]. However, despite its importance there has been relatively little
work on animal-plant interactions in tropical forest remnants [but see 1].
In this study we investigate seed dispersal and regeneration processes of forest trees
comparatively in continous forest and forest fragments in Kakamega Forest, Western Kenya. In
our first field season 9 study plots of 1 ha each were established with four situated in the 8600 ha
continuous main forest block and five situated each in one of five peripheral forest fragments with
sizes ranging from 100 ha to 1500 ha. Three of the study plots are situated within the biodiversity
observatories of BIOTA East (i.e. Buyangu Hill, Mukanku and Kisere).
Our first aim is to document changes in diversity and abundance of animal dispersers
focusing mainly on birds. Kakamega Forest is well known for its rich and unusual avifauna, with
the 194 forest-dependant bird species including 40 Guinea-Congo Forest biome species and 33
Afrotropical Highland biome species [8]. A recent study showed that Kakamegas peripheral
fragments have already lost several forest bird species since their separation from the main forest
block [9]. For our study we started a monthly monitoring scheme of the diversity and abundance
of the frugivore community using point counts, both in our continous forest and fragment plots.
With these data we will be able to understand whether important functional groups such as certain
168
frugivorous dispersers are missing from forest fragments [10]. Parallel, we have started
monitoring the diversity, abundance and phenology of fruiting trees, shrubs and lianas at the same
sites. So far, fruiting has been low with mainly Ficus trees being a major fruit resource for the
frugivore community.
Beyond the diversity level, we secondly seek to determine whether forest fragmentation
affects seed dispersal and regeneration of tree species. Because the youngest plants in a previously
fragmented forest are mainly the result of regeneration from seeds after the fragmentation process
has occurred, density and pattern of seedlings may provide clues to the possible effects of
fragmentation on tree regeneration. Therefore, a first inventory of forest regeneration was carried
out by mapping seedling recruitment and regeneration in 90 1 m2 seedling plots in each of the
continuous forest and forest fragment plots. Seedlings were identified and counted and their age
estimated using the number of leaves, stem height and stem characteristics. First results indicate
that seedling diversity appears not to be related to fragment size or time of their isolation from the
main forest block. However, there seems to be a relation to management system (with higher
diversity at sites under Kenyan Wildlife Service compared to under Forest Department) and to
disturbance level with highest seedling species numbers in the low disturbance - sites Buyangu
and Kisere Forest.
In a next step, we will now look behind the potential causes for differential seedling
recruitment and study seed dispersal and regeneration of a few selected primary tree species
such as Prunus africana, Olea capensis and Antiaris toxicaria. We will will carry out tree
observations, identify seed dispersers, and quantify dispersal rates of individual trees in the
same continuous forest and forest fragment plots. We will also quantify seedling recruitment
and mortality due to predation and herbivory.
References:
1. Laurance, W.F., and Bieregaard, R.O. 1997. Tropical forest remnants. Ecology, management, and
conservation of fragmented communities. University of Chicago Press.
2. Tilman, D. 1999. Ecology 80: 455-1474.
3. Chapin, F.S.III, Zavaleta, E.S., Eviner, V.T., Naylor, R.L., Vitousek, P.M., Reynolds, H.L., Hooper,
D.U., Lavorel, S., Sala, O.E., Hobbie, S.E., Mack, M.C., and Diaz, S. 2000. Nature 405: 234-242.
4. Hubbell, S.P. 1979. Science 203. 1299-1309.
5. Bleher, B., and Böhning-Gaese, K. 2001. Oecologia (in press)
6. Bond, W.J. 1995. Pp. 131-146. in Lawton, J.H. & May, R.M. (eds). Extinction rates. Oxford University
Press, Oxford.
7. Laurance, W.F., Ferreira, L.V., Rankin-De Merona, J.M., Laurance, S.G., Hutchings, R.W., and
Lovejoy, T.E. 1998. Conservation Biology 12: 460-464.
8. Bennun, L., and Njoroge, P. 1999. Important bird areas in Kenya. Nature Kenya
9. Brooks, T.M., Pimm, S.L., and Oyugi, J.O. 1999. Conservation Biology 13: 1140-1150.
10. Symstad, A.J., Tilman, D., Willson, J., and Knops, J.M.H. 1998. Oikos 81: 389-397.
169
Project ID: 01LC0025 (BIOTA East Africa E12)
01.04.2001.-31.03.2004
MONTANE FOREST ECOSYSTEMS IN KENYA - DIVERSITY AND POTENTIAL
FOR USE
R W. Bussmann1 , S. Kiefer1 , R. Njeri, G. Njoroge, S. Mathenge, Mutiso, J. Onyango2
1
Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Universitätsstr. 30, 95440 Bayreuth,
Germany
2
Department of Botany, Maseno University, Private Bag, Maseno, Kenya
rainer.bussmann@uni-bayreuth.de; www.biota-africa.org
Key words: Montane forests, ecology, regeneration, vegetation, ethnobotany
Abstract:
Objectives of the proposed project are: First the investigation of the dynamics and stability of
the montane forests. How do different types of disturbance (timber extraction, and slash- and
burn, high game and livestock populations) affect biodiversity. The second focus centers on
the current land-use practices and attempts to evaluate which sustainable use of these
resources would be possible. Special attention will be given to the use of non-timber products
(particularly medical plants), and the carrying capacity with relation to game and livestock
populations.
Project and first Results:
Despite their enormous biological diversity and their exceptional economic and ecological
importance as water catchments, erosion barriers and source of timber and non-timber
products, tropical montane forests have so far been overshadowed by the interest of
governments, public and scientific community in tropical lowland forests. Apart from the
Biodiversity Convention, the “Mountain Agenda“ of the 1992 Rio Conference indicated the
high priority in mountain region conservation. Little information however exists about this
ecosystem. Few neotropical studies have been compiled. Most research focuses still on
lowland rainforests or the alpine zones. Even large studies as „Ecoandes“ in Colombia touch
the mountain forests only marginally. Our own long-term studies about the phytosociology
and regeneration of the forests of Eastern Africa are a sound base for further ecological
investigations. The research deficit in tropical mountain forests becomes apparent particularly
with regard to forest regeneration. There are almost no studies on regeneration- and
succession processes after natural or human disturbances. Only in Eastern Africa, our
previous work provides detailed information about forest regeneration. and the germination
and growth of important tree species.
Kakamega and Mount Kenya were declared forest reserves in 1933 and 1932. At this time,
there were still about 24000ha primary forest in Kakamega. Meanwhile, only 13000 ha
(1988!) of fragmented forests are left. 10-20% of the fauna are regarded as endemic. Kenya
Indigenous Forest Conservation Project (KIFCON) estimated a value of US$ 2.000.000 for
annually harvested forest products, leading to high overharvesting. In the last 26 years
Kakamega lost about 50% of its standing timber. 60 years of absolute protection would be
necessary to reach the 1965 level again – impossible in a region with high demand for forest
products.
Little knowledge exists about Kakamega. Few data on vegetation, tree species diversity and
fragmentation are available, and little data on regeneration processes.
170
Research on use of non-timber products, particularly medicinal species is extremely important
to indicate alternatives to timber use and mining, and can thus provide a base for sustainable
use projects. Such studies need a high degree of trust from the side of the local population,
and thus should be conducted by students from the host country.
Main topic of research are the disturbance of the dynamic species composition in various
forest communities, the regeneration potential of the biocoenoses and the way of regeneration,
and the use potential of the mountain forests for the local population. The succession
processes are studied on selected plots with clear natural (treefall, wind, fire) or human
(cutting, slash- and burn, pasture use) disturbance. Main focus are anthropogenous
disturbance, as natural processes have been studied by our group already in detail. Particularly
important will be to focus on possibilities for sustainable use – how fast can forests regenerate
to a climax community? Is high species diversity necessary for maintaining stable forest
communities? Fire is of special importance, as this is the most common mean of forest
destruction. Timber use and pasture are additional focuses. The possibility for regeneration of
disturbed areas are studied with fencing experiments and grazing of selected plots. This will
give base data about the vegetation and its possible uses, and will lead to suggestions for
management. Additionally, population structure, Regeneration and Ecology of important
timber and non-timber species are investigated along altitudinal transects.
The intense use of non-timber products is extremely important for rural populations in large
parts of the tropics. Apart from forest products providing food, building materials or fodder,
many species serve as medicinal plants. 80-90% of the population uses such traditional
medicines. The knowledge about the use is handed from Generation to generation. In recent
times, this knowledge transfer is interrupted more and more often, as old healers die, and no
apprentices are available. This is particularly pitiful as this sector provides very interesting
potential for the sustainable forest use. The documentation of medicinal plants, their uses,
possibly contents and their ecology are the second part of the research project. Two Kenyan
Ph.D. students funded by the project conduct this work in the Kakamega and Mount Kenya
Regions. A final goal of the work is to provide a public medicinal plant herbarium and a
“medicinal plant guide“ for the training of health assistants in the local communities. This is
an important focus in the establishment of a Botanical Garden at Maseno University, for
which Maseno University has set aside 15 hectares of land. Maseno University contributes to
this task not only by providing the land but also by its pertinent botanical and horticultural
knowledge, by investment of manpower and by offering facilities for external researchers to
use the plant material and other facilities in the garden. The goals of Maseno University
Botanical garden are to grow, study and promote tropical flora with emphasis on woody
native Kenyan species in an educationally useful setting as teaching field laboratories for
students‘ individual ecological and conservation projects and to provide an information
resource for government, industry, science and the community for practical utilization of
forest resources in human development. This is in particular necessary because of the almost
complete deforestation of the country, leaving not more than 2% of the country as living
and/or gazetted forests. The destruction of plants with high medicinal value is taking place at
faster rate. This needs to be mitigated by ex-situ culture of medicinal plants which after
developing appropriate cultivation techniques could then be made available to the traditional
healers, thus Ethnobotany are one of the major goals of the garden.
171
Project ID: 01LC0025 (BIOTA East Africa, Yemen E13-E16)
BIOTA-EAST
YEMEN-PROJECT-GROUP:
BIODIVERSITY AND ECOLOGY OF THE PALAEO-AFRICAN REFUGE AREAS
IN SOUTHERN ARABIA AND ON SOCOTRA (REPUBLIC OF YEMEN)
Since the Arabian Peninsula was separated from the African continent not earlier than in the
Oligocene, E Africa and the Arabian Peninsula had a common tropical flora, vegetation and
fauna for a long time. At the same time, migration and exchange between the xerotropical
floras and palaeotropical faunas of Asia and Africa were possible across the Arabian
Peninsula. Due to the aridisation of the entire region in the late Tertiary, the palaeo-African
vegetation and its fauna was then forced into the south of the Arabian Peninsula, isolated from
Africa, with increasing aridity finally fragmented and delimited to refuge areas. In the
monsoon-affected mountainous areas of the south coast of the Arabian Peninsula and on the
island of Socotra, such island-like relict vegetation complexes rich in endemics and with close
floristic affinities to NE tropical Africa have survived to the present. They are bioclimatically
and ecologically conspicuously differentiated and comprise shrub formations, as well as
deciduous and semi-deciduous woodlands of various composition. Similar to the situation
regarding the flora, the fauna of S Arabia consists of a unique and complex mix of widespread
afrotropical species, highly specialized endemics and remnant populations of palaearctic species.
Five representative palaeo-African refugia have been selected to investigate plant diversity
and structure of xerotropical montane communities in southern Yemen and on Socotra by
means of standardized monitoring sites. A main objective of the Yemen project group is to
obtain information about the history of habitat fragmentation and its present effect on genetic
diversity in selected groups of plants, insects and vertebrates. Genetic comparison of isolated
populations is used to estimate the time frames of their historical dispersal and diversification.
The floristic and vegetational data will provide new insights into the biodiversity and
biogeographical position of this little known but biogeographically very important region. A
first documentation of the value, importance and ecology of these ecosystems finally will help
to estimate the present state of desertification and to develop management plans for the
preservation and sustainable use of these unique xerotropical communities.
The selected areas are:
(1) Hawf Mountains, Governorate Al-Mahra: The coastal escarpments shelter the most
extended closed woodlands in Yemen, partly comprising endemic Anogeissus woodland. As a
species rich area with considerable regional endemism it has been recommended by the
Environmental Protection Authority of the Republic of Yermrn for protection. Important rural
economy.
(2) Ras Fartak Mountains, Governorate Al-Mahra: A climatically transitional mountainous
area with very sparse and low woody, partly succulent vegetation cover, but with patches of
semideciduous Anogeissus woodland in the most favourable sites in the summit area at
altitudes of 700-950 m. Extensive pasture economy.
(3) Kor Seiban area, Governorate Hadhramaut: A summit plateau at altitudes of 1800-2000 m
with sea facing vertical escarpments and furrowed by numerous wadis. Remnants of semideciduous woodland have survived on some slopes and in wadis. Pasture economy.
(4) Jabal Urays, Governorate of Abidjan: The climatically most favoured mountainous area in
the coastal SW of Yemen, with important relict communities and high local endemism.
Pasture economy.
(5) Socotra, Shihali Peaks, Governorate of Hadhramaut: Species-rich, climatically favoured
woodland communities. Pasture economy.
172
Partners in the Republic of Yemen:
Dr. Mohamed Ali Hubaishan, Agricultural Research Station, Agricultural Research &
Extension Authority (AREA), Eastern Coastal Branch, Ministry of Agriculture and Irrigation,
P.O. Box 8073, Mukalla
Dr. Abdul Nasser Al Gifri, University of Aden, P.O. Box 6312, Khor Makssar, Aden
Prof. Dr. Abdul Karim A. M. Nasher, Faculty of Science, Sana'a University, P.O. Box 12231,
Sana’a
Fig. 1. Map showing the five selected refugia in the coastal mountains of southern Arabia and
on Socotra (1 = Hawf Mountains, Gov. Al-Mahra, 2 = Fartak Mountains, Gov. Al-Mahra, 3 =
Kor Seiban area, Gov. Hadhramout, 4 = Jabal Urays, Gov. Abyan, 5 = Socotra, Shihali Peaks,
Gov. Hadhramout).
173
Project ID: 01LC0025 (BIOTA East Africa, Yemen E13)
1.3.2001 – 31.3.2004
PHYTODIVERSITY AND VEGETATION TYPES OF REPRESENTATIVE PALEOAFRICAN REFUGIA IN THE COASTAL MOUNTAINS OF SOUTHERN YEMEN
AND ON SOCOTRA (REPUBLIC OF YEMEN)
Norbert Kilian, Peter Hein, Mohamed Ali Hubaishan and Harald Kürschner
N. Kilian, ZE Botanischer Garten und Botanisches Museum Berlin-Dahlem, Freie Universität
Berlin, Königin-Luise-Str. 6-8, 14191 Berlin; n.kilian@bgbm.org
P. Hein, H. Kürschner, Institut für Biologie, Systematische Botanik & Pflanzengeographie,
Freie Universität Berlin, Altensteinstr. 6, 14195 Berlin; kuersch@zedat.fu-berlin.de,
phein@mail.bgbm.fu-berlin.de
M. A. Hubaishan, AREA Mukalla, P.O. Box 8073, Mukalla, Republic of Yemen
___________________________________________________________________________
Key words: Yemen, Anogeissus woodlands, tropical vegetation relics, fragmentation, flora.
Abstract:
As the results of the first six weeks' field campaign in September and October, first
standardized monitoring sites on which the Yemen Project Group combines their research
were established in three of the five selected refugia, and 650 plant samples for the molecular
analyses of subproject E15 were provided. Determination, analysis and documentation of the
floristic inventory as well as the present vegetation complexes of the palaeo-African relict
vegetation in the S Yemeni-Socotran refugia was started, focusing on the BauhiniaTarchonanthus woodland relics in the Kor Seiban refugial area in the governorate of
Hadhramout and on the closed Anogeissus dhofarica woodlands in the two refugia in the
governorate of Al-Mahra. Instructively illustrating the post-Tertiary fragmentation of the
xerotropical plant cover in southern Arabia, preliminary results of the vegetational and
floristic study of the Anogeissus monsoonal woodland remnants in the Hawf and Fartak
mountains are provided.
Results:
The monsoonal woodland on the Arabian Peninsula is restricted to a small area along the
central south coast. It extends from Jabal Samhan, at c. 55°E, in the province of Dhofar of the
Sultanate of Oman to the Ras Fartak mountains, at c. 52°10'E, in the Governorate of Al Mahra
of the Republic of Yemen. The still little known main part of c. 10 000 ha in Yemen is
confined to the coastal escarpments of the Hawf Mts. between Al Fatk at 52°44'E° and the
border to Oman (our selected refugial area no. 1). To the larger extent it is an AcaciaCommiphora woodland, only on the climatically and edaphically most favoured sites, at
altitudes of 300-900 m and in about a fourth of the total woodland area, a closed woodland
dominated by the endemic Combretaceae tree species Anogeissus dhofarica and with a closed
canopy at about 7-8 m height exists. All eight species of Anogeissus are trees of rain green
tropical woodlands and forests. A. leiocarpa occurs in Africa from NE Ethiopia to Senegal,
five species occur from India to SE Asia, two species are endemic to S Arabia. Besides A.
dhofarica this is A. bentii, which is restricted to gallery woodlands of wadis in Hadhramout.
Both species are relics of the Tertiary belt of (semi)deciduous tropical forest and woodland
ranging from S Asia to Africa across the south of the Arabian Peninsula.
According to our preliminary results, the Anogeissus woodland is the by far most species-rich
woodland community in the Arabian Peninsula, with c. 90 species per 1000 m2
174
Table 1. Tree and shrub species of Anogeissus
woodlands in Al-Mahra, Yemen.
Hawf Fartak
Acacia senegal
+
+
Acacia etbaica subsp. uncinata
+
+
Acacia nilotica subsp. indica
+
Acokanthera schimperi
+
Allophyllus rubifolius
+
Anogeissus dhofarica
+
+
Azima tetracantha
+
+
Blepharis dhofarensis
+
Blepharispermum hirtum
+
Cadia purpurea
+
Carissa edulis
+
Cocculus balfourii
+
+
Commiphora kua
+
+
Commiphora gileadensis
+
+
Cordia perrottetti
+
Croton confertus
+
Delonix elata
+
Dichrostachys cinerea
+
Dodonaea angustifolia
+
+
Euclea schimperi
+
+
Euphorbia smithii
+
Ficus ingens
+
Ficus sycomorus
+
Ficus vasta
+
+
Flueggea virosa
+
+
Grewia bicolor
+
Grewia villosa
+
+
Hildebrandtia africana
+
+
Hybanthus durus
+
+
Jasminum grandiflorum
+
+
Jatropha dhofarensis
+
Justicia areysiana
+
+
Lannea triphylla
+
Lantana sp. YP460
+
+
Maytenus dhofarensis
+
+
Olea europaea subsp. cuspidata +
+
Pavetta longiflora
+
+
Pistacia falcata
+
Premna resinosa
+
Rhus flexicaulis
+
+
Rhus somalensis
+
+
Ruttya fruticosa
+
Tarenna graveolens
+
+
Woodfordia uniflora
+
Zygocarpum dhofarense
+
Fig. 1. Small Anogeissus woodland patch on Jabal Fartak.
on average (Table 1). Towards the upper edge
of the woodland belt, evergreen tree species are
mixed into both the Acacia-Commiphora and
the Anogeissus woodland. A separate sclerophyllous woodland belt as, e.g., the Olea-Barbeya woodland in the Asir Mts. of Saudi Arabia
is not formed in Al-Mahra. A hundred km
farther west and entirely isolated from these
main stands, the Fartak mountains (our refugial
area no. 2) shelter some outposts of Anogeissus
woodland. The effect of the SW monsoon is
drastically reduced there and the semi-deciduous Anogeissus woodland type (Table 1) is
confined to small or even tiny patches (ranging
from 80 to c. 2000 m 2) in moist niches at
altitudes of 700-950 m (Fig. 1), which are,
however, similarly species-rich as in the Hawf
Mts. Initial floristic analyses of the Al-Mahra
refugia result in a number of species not
hitherto known from Yemen (Table 2).
Table 2. Initial list of new records for the vascular plant flora of Yemen; 1 = Hawf area, 2 = Fartak area;
vouchers at B.
Alectra parasitica Hochst. (1), Becium dhofarense Sebald (1), Blepharis dhofarensis A.G. Mill. (1),
Blepharispermum hirtum Oliv. (1), Centaurea dhofarica Baker (1), Ceratopteris cornuta (P. Beauv.) Lepr. (1),
Cibirhiza dhofarensis P. Bruyns (1), Dhofaria macleishi A.G. Mill. (1), Dichrostachys cinerea (L.) Wight &
Arn. (1), Dyschoriste dalyi A.G. Mill. (1), Euphorbia orbiculifolia S. Carter (1), E. smithii S. Carter (1),
Gladiolus ukambanensis (Baker) Marais (1), Gossypium stocksii Masters (1, 2), Habenaria malacophylla
Reichenb.f. (1), H. myodes Summerh. (2), Jatropha dhofarensis Radcl.-Sm. (1), Kleinia saginata P. Halliday
(1), K. squarrosa Cufod. (1, 2), Lannea triphylla Engl. (1), Lavandula dhofarensis A.G. Mill. subsp. dhofarensis
(1, 2), Maytenus dhofarensis Sebsebe (1, 2), Portulaca dhofarica M. G. Gilbert (1), Poskea socotrana (Balf.f.)
Taylor (2), Pulicaria nobilis Gamal-Eldin (2), Remusatia vivipara Schott (1), Rungia parviflora Lindau (1),
Suaeda moschata A.J. Scott (2).
175
Project ID: 01LC0025 (BIOTA East Africa, Yemen E14)
1.3.2001 – 31.3.2004
ECOLOGICAL CAUSES OF BIODIVERSITY IN THE COASTAL MOUNTAINS OF
SOUTHERN YEMEN AND ON SOCOTRA (REPUBLIC OF YEMEN)
B. Mies, G.-B. Feige
Universität Essen FB9, Botanik/Pflanzenphysiologie, 45117 Essen, Germany
bruno.mies@uni-essen.de, benno.feige@uni-essen.de, www.biota-africa.org
Key words: South Arabia, vegetation, relic forest, Anogeissus dhofarica, microclimate, dewfall, ecology, ecophysiology
Abstract:
The ecological analyses of the vegetation types aim at morpho-functional adaptations and
general patterns concerned with the xerotropical plant cover. These concern especially water
economy, phytomass and specific productivity of the vegetation types. Pedological
examinations complete this view determining the influences of edaphic factors to vegetation.
The causal explanation of vegetation ecology results in the explanation of the actual
desertification facing the thread of habitat destruction and loss of biodiversity. This gives a
scientific basis to assist an ecologically sustainable development. An evaluation is aimed at
the ecological potential of nature resources and the intensity of human use or exploitation. It
reveals the qualification of vegetation types important to an indigenous economy and to the
influences on specific degradation states as well as the actual risks for the ecosystems (fig. 1).
Results:
During the South Arabian Field Campaign in Oct. 2001 and at the end of the monsoon season,
microclimatic parameters and respective plant responses have been measured. Field
Measurements were taken at the sights of permanent plots agreeing to the flora and vegetation
studies of Sub-Project E13. Primary data add some insight into the puzzle of understanding
and approaching an ecosystem undergoing desertification. As rain-fall was exceptionally low
in 2001 - and the local population could not even yield one drop of cistern water - dew-fall
seemed to provide the regular precipitation and to contribute a major supply to the vegetation.
This subsidized pre-project measurements from Soqotran mountain ranges (fig. 2). The
extensive use of the chlorophyll fluorescence technique enlightened photosynthetic responses
on individual and vegetation level. Diverse ecological patterns have been studied in detail at
an Anogeissus dhofarica spot at Site 1 in the Hawf area. Further field data have been recorded
in the coastal mountain ranges at Ras Fartaq and at Damqaut.
Representative plant and soil probes have been taken for laboratory measurements. Plant
material was dedicated to quantify its contents of chlorophyll and minerals, soil samples to
their textures, nutrients and to the C/N analysis. Some representative specimens of dominant
species have been transplanted to the greenhouses to give access to more sophisticated
ecophysiological studies.
176
Fig. 1: Remnants of a formerly Dracaena cinnabari dominated woodland on the montain
slopes of eastern Soqotra.
Fig. 2: Insolation, air temperature and humidity during a day course with nightly heavy cloud
formation at the Aduno ridge on Hajhir Mountains (Soqotra).
177
Project ID: 01LC0025 (BIOTA East Africa, Yemen E15)
1.5.2001 –31.3.2004
PHYLOGEOGRAPHIC AND POPULATION GENETIC ASPECTS OF THE
PHYTODIVERSITY IN PALAEO-AFRICAN REFUGIA OF THE COASTAL
MOUNTAINS OF SOUTHERN YEMEN AND SOCOTRA (REPUBLIC OF YEMEN)
J. Meister1 , Ch. Oberprieler2
1)
Institut für Geobotanik und Botanischer Garten, Martin-Luther-Universität Halle/
Wittenberg, Am Kirchtor 1, 06108 Halle/ Saale, Germany
2)
ZE Botanischer Garten und Botanisches Museum Berlin-Dahlem, Freie Universität Berlin,
Königin-Luise-Str. 6-8, 14191 Berlin, Germany
meister@botanik.uni-halle.de; c.oberprieler@bgbm.org, www.biota-africa.org
Key words: Arabian peninsula, phylogeography, habitat fragmentation, genetic diversity,
RFLP, AFLP
Abstract:
Using molecular genetic methods (PCR-RFLP analysis of the chloroplast genome, AFLPfingerprinting) in five representative species or species groups of the palaeo-african refugia of
Southern Yemen and Socotra, subproject E 15 aims at both the reconstruction of the temporal
and spatial fragmentation processes which have led to the formation of these refugia and the
effect of habitat fragmentation on the genetic diversity of the populations involved. While for the
phylogeographical part of the project the assessment of chloroplast haplotype variation as
revealed by PCR-RFLP analysis is planned, the genetic analyses of fragmented populations will
be based on the AFLP fingerprint technique. A short overview of molecular genetic methods and
plant groups involved in the analyses of subproject E 15 along with first results of the PCR-RFLP
screening phase in Maytenus senegalensis (Celastraceae) is provided.
Subproject E15 of the Yemen-Project-Group within BIOTA-East uses molecular genetic methods
to reconstruct the chronological fragmentation of the palaeo-african refugia under study. The
elaboration of a hypothesis concerning the temporal and spatial development of these habitats will
contribute to a discussion of aspects of taxonomic differentiation, floristic evolution (together with
subproject E13), functional morphological adaptation (together with subproject E14) and will
enable the comparison with comparable faunistic data (subproject E16). Additionally, population
genetic analyses will be done to study the effects of fragmentation of populations on their genetic
structure and diversity.
Molecular genetic methods involved:
• cpDNA PCR-RFLP: Using a set of universal primer pairs [1, 2, 3] non-coding regions
of the chloroplast genome are amplified via polymerase chain reaction (PCR) and sequence and length polymorphisms are detected by digestion of the PCR-products with
a set of restriction endonucleases followed by electrophoresis of resulting fragments.
Phylogeographical interpretations are based on phylogenetic reconstructions of
chloroplast haplotype evolution.
• AFLP-fingerprinting: The digestion of a total extract of genomic DNA with specific
restriction endonucleases, followed by two selective amplifications of subsets of the
population of restriction fragments and their electrophoretic analysis [4, 5] yields
individual-specific band patterns (fingerprints) that are analysed in an population
178
genetic framework (e.g. Analysis of Molecular Variance, AMOVA [6]) to study
genetic variation within and between populations.
Species and species groups under study:
• Maytenus senegalensis, M. dhofariensis (Celastraceae)
• Gossypium stocksii, G. incanum, G. areysianum (Malvaceae)
• Launaea crassifolia (Asteraceae)
• Euryops arabicus (Asteraceae)
• Euclea schimperi (Ebenaceae)
• Justicia areysiana (Acanthaceae)
Results:
Of the 16 pairs of cpDNA primers tested, different numbers were found to successfully amplify
non-coding regions of the chloroplast genome in each of the species involved in the study.
Starting the screening process with Maytenus senegalensis, the amplified fragments were digested
with four restriction enzymes (AluI, BsuRI, RsaI, TaqI) and the resulting fragments separated by
electrophoresis in agarose (2%) and PAA (8%) gels. While the screening is still in process
presently with a small test population of 10 individuals from different Yemeni provenances, until
now we found two polymorphisms and consequently three chloroplast haplotypes in this species:
Digestion of PCR product psbC-trnS (c. 1650 bp long) with TaqI resulted in a fragment of 370
bp vs. 350 bp length in one N Yemen individual, while digestion of PCR product trnK1-trnK2 (c.
2580 bp long) with TaqI revealed a difference between plants from N and S Yemen populations
(240 bp vs. 280 bp fragment; Figure 1).
The screening is presently continued for all other PCR products and the other species under study.
Once established the relevant fragment/restriction enzyme combinations are used in analyses of
whole populations.
Fig. 1.: Screening of chloroplast DNA variation in
Maytenus senegalensis. The figured gel photograph
demonstrates the occurence of two different PCRRFLP patterns of the non-coding region trnK1trnK2 (c. 2580 bp long) when digested with restriction enzyme TaqI. M: 100-bp molecular weight
marker; 1-6, 8: N Yemen populations; 7: Ras Fartak
Mountains population; 9: Hadramaut population.
References:
1. Taberlet, P. et al. 1991. Plant Molecular Biology 17, 1105-1109.
2. Demesure, B. et al. 1995. Molecular Ecology 4, 129-131.
3. Dumolin-Lapegue, S. et al. 1997. Molecular Ecology 6, 393-397.
4. Vos, P. et al. 1995. Nucleic Acid Research 23, 4407-4414.
5. Sharbel, T. F. In: Epplen, J.T. & Lubjuhn, T. (eds.), DNA profiling and DNA fingerprinting, Pp.
177-194. Basel, Boston.
6. Excoffier, L. et al. 1992. Genetics 131, 479-491.
179
Project ID: 01LC0025 (BIOTA East Africa, Yemen E16)
1.04.2001-31.03.2004
EVOLUTIONARY HISTORY AND PHYLOGEOGRAPHY OF SOUTH ARABIAN
FAUNAL ELEMENTS
C. Klütsch1 , C. M. Naumann1 , M. A. Hubaishan2 , A. K. Nashr3
1
Zoologisches Forschungsinstitut und Museum Alexander Koenig, Adenauerallee 160, D53113 Bonn, Germany
² Director AREA Mukalla, POB 8073, Mukalla, Republic of Yemen
³ University of Sanaa, Department of Biology, Chair of Zoology, Sanaa, Republic of Yemen
c.kluetsch.zfmk@uni-bonn.de, c.naumann.zfmk@uni-bonn.de, karimnasher@yahoo.com
Key words: South Arabia, biogeography, phylogeography, endemism, population genetics,
evolutionary history, Lepidoptera, Reissita simonyi, Lasiommata felix, Amphibia, Hyla
savignyi, Reptilia, Varanus yemenensis, DNA-microsatellites
Abstract:
South Arabia is inhabited by a highly specialized fauna and flora of peculiar interest to the
biogeographer and evolutionary biologist. The rich biodiversity of the area is increasingly
endangered by various hazards, most of which are related to human population growth and
its consequences. Our project aims at analysing the origin of various endemic faunal
elements, the amount of gene flow between partly highly separated populations and at
identifiying viable populations that might be suited for population management and the
establishment of permanently protected areas. During two periods of field work (June and
September/October 2001) we have been able to 1. provide a clear picture of the present
distribution of Reissita simonyi and closing a considerable gap in the distribution of the two
subspecies of the species (appr. 700 samples), 2. to document and sample about ten
populations of Lasiommata felix (100 samples) and Hyla savignyi (200 samples) and 3. to
collect first samples of Varanus yemensis and related taxa from other parts of the
Afrotropical region. In addition we have been able to sample about twenty populations of the
larval foodplant of Reissita simonyi, i.e. Maytenus senegalensis (Celestraceae) to be analyzed
in subproject BIOTA E15. Genetic markers for population genetic analyses (DNAmicrostaelliutes) of Hyla savignyi have been optimized and similar work has been started for
Reissita simonyi.
Results:
The South Arabian fauna consists mainly of Afrotropical elements and a few Palaearctic
faunal elements. The amount of endemism is extremely high in the area (e.g. 13 endemic
species of birds). Little is known on the temporal and spatial differentiation of these elements.
Such questions therefore form the core part of our project. Of the four target species selected
for the project two are of Afrotropical origin (Reissita simonyi, Varanus yemensis) and two of
Palaearctic origin (Lasiommata felix, Varanus yemensis). Thus, our analysis includes a
vertebrate and an invertebrate species of both categories.
Aims and Scopes
- to analyse the faunal history of southern Arabia, i.e. processes related to local colonization
and subsequent differentiation of Afrotropical and Palaearctic faunal elements as a result of
tectonic drift, post-glacial separation and subsequent habitat isolation due to regional
desertification in the recent past.,
180
- to determine the duration of population isolation using genetic data and establishing a
molecular clock for such events,
- to provide basic data for the establishment of priority and target areas for nature
conservation in the south-western parts of the Arabian peninsula.
Reissita simonyi
So far, two distinct and disjunct subspecies of this ecologically highly specialized taxon have
been identified: R. s. simonyi along the southern coast (Oman, eastern governorates of
Yemen; large, dark blue sheen, both sexes monomorphic) and R. s. yemenicola (along western
escarpment from the Hejjaz to Taiz; considerably smaller, forewing with greenish blue sheen,
males dimorphic, females monomorphic). The disjunction (distributional gap) between these
two subspecies amounted to about 700 kms so far. In 2001 we have been able to discover a
population of R. simonyi near Korseban (Prov. Hadramaut) which fills the middle of the
present gap. Larval samples for molecular analysis have been taken from here and about 18
further populations of both subspecies in Yemen (about 700 samples). The phenotype of the
intermediate population is still unknown and will be the target of further analyses during
forthcoming field work. We assume that further investigations will still narrow the gap
between the populations enabling us to estimate whether or not there is still gene flow
between the two populations. We have also been able to document in detail the range of R. s.
yemenicola and its larval host-plant, Maytenus senegalensis (Celastraceae). Of the latter,
population samples have been secured for project Biota E15 (Dr. Oberprieler, Berlin)
enabling our two research teams to compare data on gene flow in this phytophagous insect
and its respective larval food-plant. Population samples of R. simonyi yemenicola have now
been documented from the border with Saudi Arabia in the north down to Jabal Sabr (near
Taiz), with maximum distances of 10-50 kilometers between locations. A number of localities
has been recorded for the first time.
Hyla savignyi, Lasiommata felix
Similarly, we have been able to document the range of these two taxa to a rather detailed
scale, again from the north (Jabal Rhaza) to Jabal Sabr in the south. The number of population
samples taken so far from both taxa is promissing and will allow further sampling during the
next two years.
Molecular work
The development of DNA-microsatellites has been started for Reissita simonyi. In addition,
DNA-microsatellitesd have een tested for Hyla savignyi and the conditions for PCR work
have been optimized.
References:
1. Arens, P., Van´t Westende, W., Bugter, R., Smulders, M.J.M., Vosman, B. 2000. Molecular
Ecology 9, 1944-1946
2. Naumann, C.M., Edelmann, A. 1984. Fauna of Saudi Arabia 6, 473-509
181
Project ID: 01LC0001
1.1.2001-31.12.2003
BIODIVERSITY OF SELECTED MODEL GROUPS OF THE PHYTOPLANKTON
IN STANDING INLAND WATERS OF KENYA
A. Ballot1), K. Kotut 2), L. Krienitz1)
1)
2)
Leibnitz Institute of Freshwater Ecology and Inland Fisheries, D-16775 Neuglobsow, Germany
Kenyatta University, Department of Botany, P.O. Box 43844, Nairobi, Kenya
ballot@igb-berlin.de, kie@nbnet.ke, krie@igb-berlin.de
Key words: biodiversity, phytoplankton, Kenya, Rift Valley lakes, reservoirs, small water bodies
Abstract:
The objective of the project is to investigate the biodiversity of phytoplankton communities in
standing inland waters of Kenya with respect to the following items: (i) selection of priority
waters representing model ecosystems of different phytoplankton biodiversity, trophic state and
human use, (ii) investigation of the physico-chemical conditions of the waterbodies, (iii) detailed
inventory of the species stock of model groups of phytoplankton with high ecosystemare relevance
and (iv) conclusions concerning their functional role in these waters. During the field work
various Rift Valley lakes, reservoirs and small standing waters were investigated. The chemistry
of the water bodies showed a great variety. Significant differences were also found in the
composition of the phytoplankton communities. The phytoplankton biodiversity in Kenyan
standing waters is higher than expected, especially in the diversity of picoplanktic (< 3 µm) and
nanoplanktic algae (< 20 µm).
Results:
East Africa suffers from a deficit of high quality and easily accessible water resources, due to long
drought periods and increasing demographic pressures. Hence, there is an increasing need for the
evaluation and formulation of management strategies for these water bodies. The water quality
can be characterized by physico-chemical criteria and biological criteria like the phytoplankton
community structure. The importance of the phytoplankton community structure and biodiversity
can be explained by the following circumstances: (i) being the main pelagic primary producer the
phytoplankton plays a key role in standing water bodies, (ii) the structure of the phytoplankton
community, i.e. its biodiversity has a principal influence on many kinds of water utilization by
man, (iii) as a consequence of its high reproduction rates, phytoplankton gives a rapid response to
changes of environmental conditions, (iv) phytoplankton communities in general are more diverse
than other eucaryotic populations in aquatic food webs.
Despite of the importance of phytoplankton biodiversity, the investigations in this area still suffer
for various reasons: the lack of specialists of algal systematics, minor knowledge on
phytoplankton diversity in different climatic regions of the world and the sometimes necessary
expensive methods (electron microscopy and molecular biology) for correct determination. It is
estimated that so far only 10-20 % of the algal taxa are known worldwide [1]. In East Africa,
phytoplankton investigations have been going on for about 100 years [2]. However, the
investigation of standing waters were mainly focused on large lakes like Lake Victoria or Rift
Valley lakes [3,4,5]. About reservoirs and small water bodies only few data are available [6,7,8].
An extensive working program will be conducted in co-operation with: Kenyatta University
Nairobi (KU); Kenya Wildlife Service (KWS); Kenyan Ministry of Education, Science &
Technology (MEST); Tana & Athi Rivers Development Authority (TARDA); Kenya Electricity
Generation Naivasha and Masinga (KenGen); Nairobi- Baringo- Koibatek City Councils (NCC,
BCC, KCC); National Museum of Kenya (NMK); International Center for Research of
Agroforestry (ICRAF). The approaches are to link with the activities of the BIOTA-projects in
Africa and the BIOLOG-project AlgaTerra.
182
During our field work interesting water ecosystems will be selected. The selection includes
undisturbed and anthropologically influenced waters. Each sampling site will be characterized by
its (i) geomorphological features (geographic position, morphometry, catchment area, inflowing
rivers, human impacts, macrophytes), (ii) physico-chemical parameters (water temperature, pH,
conductivity, oxygen, ammonia, nitrate, total nitrogen, phospate, total phosphorus, iron) and (iii)
biological characteristics. Water samples will be taken for quantitative and qualitative
phytoplankton analyses, single cells or single colonies of interesting algae will be isolated for unialgal cultures for algae systematics, ecophysiological and biotechnological purposes, a checklist
of phytoplankton species and their abundance in the investigated waters will be established. The
main focus is on cyanobacteria, coccoid green algae (Chlorophyta) and centric diatoms
(Bacillariophyta) as remarkable producers of biomass and groups with the highest biodiversity.
The preliminary investigations will also include Desmidiaceae, Euglenophyceae and
Dinophyceae.
During the first sampling trip in June 2001 24 standing waters were investigated, e. g. Rift
Valley lakes: including the freshwater lakes Naivasha, Ol Oiden, Baringo and Ol Bolossat and
the saline lakes Nakuru, Elementeita, Bogoria and Sonachi; reservoirs: Kibutie-, Kirandich-,
Ngewa-, Ruiru-, Athi- and Masinga-Reservoirs and small standing waters: Uhuru park pond
(Nairobi), Nakuru final sewage pond, div. small standing waters in Nakuru National Park.
Table 1: Main results of the physico-chemical investigations in June 2001
Parameter
pH
Conductivity (µS/cm)
Alkalinity (mg CaCO3 /l)
NH4 -N (mg/l)
NO3 -N (mg/l)
total-N (mg/l)
PO4 -P (mg/l)
total-P (mg/l)
Rift Valley Lakes
8.9 – 10.5
355 – 60000
250 – 70000
< 0.04 – 1.1
0.9 – 7.9
2.4 – 11.3
< 0.01 – 4.5
0.2 – 18.0
Reservoirs
6.5-7.7
45 – 235
24 – 144
< 0.04 – 0.05
< 0.3 – 3.6
< 0.5 – 3.9
< 0.01 – 0.08
0.05 – 0.8
Small water bodies
8.0 – 9.3
202 – 3620
136 – 2500
< 0.04 – 0.5
2.6 – 6.1
2.7 – 15.9
0.5 – 2.3
0.5 – 4.4
The composition of the phytoplankton communities differed considerably, depending on the
character of the lake ecosystem. The alkaline Rift Valley lakes were dominated by blue-green
algae, e.g. Arthrospira fusiformis, Anabaena sp. and Anabaenopsis abijatae. The fresh water
lakes showed like Lake Naivasha either a diverse phytoplankton community dominated by
coccoid green algae e.g. Pediastrum div. sp., Scenedesmus div. sp. and Oocystis div. sp., bluegreen algae like Aphanocapsa sp. and Cyanocatena sp., Euglena div. sp. and various diatoms
or a variety of desmids (Lake Ol Bolossat). In the turbid Lake Baringo the blue-green alga
Microcystis aeruginosa and the green alga Thorakomonas sp. were found in high amounts.
Most of the investigated reservoirs were only recently filled due to the severe droughts of the
last years and were highly turbid. In this case only a poor phytoplankton diversity was found.
We expect that after settling of the silt a diverse phytoplankton community like in the clear
Kibutie-Reservoir will develop. The turbid small and shallow standing waters in Nakuru
National Park were extremely different in phytoplankton diversity: from mass developments
of only one or few taxa to high diverse communities.
References:
1. Norton T.A., Melkonian M., Andersen R.A. 1996. Phycologia 35 (4):308-326
2. Brook, A.J., Kufferath, H., Ross, R., Sims, P.A. 1957. Rev. Algol., N.S. 4: 207-233
3. Hecky, R.E., Kling, H.J. 1981. Limnol. Oceanogr. 26: 548-564
4. Talling, J.F. 1987. Arch. Hydrobiol. Beihefte 25: 229-256
5. Kebede, E., Willén, E. 1998. Algological studies 89: 63-96
6. Evans, J.H. 1962. Hydrobiologia 20: 59-86
7. Uku, J.N., Mavuti, K.M. 1994. Hydrobiologia 272: 251-258
8. Kotut, K., Krienitz, L., Muthuri, F. M. 1998. Hydrobiologia 368: 41-59
183
184
Biodiversity Informatics
185
Project ID: 01LC9901 (SysTax)
1.4.2000 – 31.10.2003
SYS TAX - ELECTRONIC DATA PROCESSING FOR RECORDING
ANALYSING BIODIVERSITY DATA WITH THE SYSTEMATIC
TAXONOMIC DATABASE SYSTEM SYS TAX
AND
AND
J. R. Hoppe and Th. Ludwig
Abteilung Systematische Botanik und Ökologie, University of Ulm, D-89069 Ulm
juergen.hoppe@biologie.uni-ulm.de; http://www.biologie.uni-ulm.de/systax
Key words: SysTax, ORACLE DBMS, biodiversity data, botanical systematics, zoological
systematics, botanical garden, herbarium management, multimedia data, biological database
system, biodiversity informatics
Abstract:
The SysTax system is a comprehensive biological database system which can be used for storing
and retrieving biodiversity data from different sources. The data can be distributed over the
World Wide Web. The system is available free of charge to all research institutions, botanical
gardens, and herbaria for online use. It has been developed since 1989 [1, 2, 3] based on the
ORACLE database management system. In this project the program modules “zoological
systematics”, “management of zoological collections”, and “information on taxa” (IOT, incl.
“ethnobiology” and “plant-animal-interactions”) will be added to the system. The program will
be used by several BIOLOG projects (esp. “EDIS” and “InfoComp-Types”) for information
storage to prove its usability as a common central database for biodiversity data.
Introduction. Within joint research biodiversity programs a large amount of primary and
secondary data on the biodiversity of plants, animals, and micro-organisms are accumulated. This
includes information relating to taxonomy and systematics, geography and geology, species
distribution, ecology, paleontology, and ethnobiology. Reference material, e.g. data on or pictures
of collection objects, text protocols and field notes, and sound or video recordings can enormously
increase the amount of information to be stored and administered. The storage and retrieval of the
data in a powerful system is indispensable to promote synergistic effects, to allow
multidisciplinary evaluation, and to make the data generally available and usable for all project
participants and the scientific community in general.
The concentration of computer resources and of database development at one site is - in our
opinion - a prerequisite for effective work and collaboration within joint projects. The
development of small local database applications often leads to systems incompatible with each
other. Because of these incompatibilities and because of the cost involved in data exchange
between different systems, such local solutions end up being much more expensive than the
development of a small number of large and powerful systems.
The SysTax system has been developed at the University of Ulm and the Ruhr-University of
Bochum since 1989 with the support of the DFG (Deutsche Forschungsgemeinschaft), the GTZ
(Gesellschaft für Technische Zusammenarbeit), the University of Ulm, and the BMBF (within the
" Federal Information System Genetic Resources - BIG; a project coordinated by Prof. Dr. Th.
Stützel, Ruhr-University of Bochum). SysTax is based on the ORACLE database management
system. From the very beginning, the entire system was implemented not only as a multi-user
system but also as a “multi-institutional” system. Several scientific institutions can store e.g. their
collection data separately but all the data can be queried together. Because of this feature the
system can be used for the installation of comprehensive information systems on the World Wide
186
Web. A “security system” allows to hide
sensitive data from being read by the public
so that an abuse of data can be prevented to
the greatest possible extent.
Results. In this project the program modules
“zoological systematics”, “management of
zoological collections”, and “information on
taxa” (IOT, incl. “ethnobiology” and “plantanimal-interactions”) are being added to the
system. SysTax is used by several projects in
the BIOLOG-Biodiversity programme (esp.
EDIS) for information storage to prove its
usability as a common central database for
biodiversity data.
Up to now, the program module zoological
systematics has been developed. It follows
the “potential taxa” concept [4] thus providing the possibility of storing several
“alternative taxonomies” as well as the
corresponding “alternative synonymies” and
the so called “concept synonyms” for a taxon
name. The program modules “ethnobiology”
and “pictures” did already exist as
prototypes. They were now combined to a
new module “information on taxa” which is
now used to store any additional (multimedia) information on animal and/or plants.
Data of the EDIS project as well as of the
InfoComp project have been imported.
SysTax –
Task Profile
Botanical and zoological systematics
and taxonomy
hierachical storage of names and (potential) taxa;
flexible synonymy; linkage of taxa with literature and
specimens; linkage of taxa with characters and
character states; taxonomical reports for direct use in
revisions
Literature
storage and administration of references; cross
referencing literature data and taxa; storage of
literature scans (plant descriptions)
Botanical Garden
storage and administration of garden accession data;
label printing; retrieval of accession lists;
creation of seed catalogues; mailing lists for seed
catalogues and seed material
Herbarium
storage and administration of herbarium label data;
administration of loans; output: herbarium labels,
revision labels, specimen lists, letters for loan,
reminders, exchange, returns
Vernacular names
Addresses
Information on Taxa (IOT)
storage and retrieval of information on taxa (plants
and/or animals): texts, pictures, movies, sound, and
OLE-objects; thesaurus for ethnobioloy and plantanimal-interactions
Queries by WWW-Forms
taxonomic reports: taxonomy, synonymy, pictures,
texts sound, movies, OLE-objects, collections (incl.
pictures of specimens)
References:
1. Hoppe, J. R., Boos, E., and Th. Stützel 1999: SysTax - ein Datenbanksystem für Systematik und
Taxonomie. In: Begemann, F., Harrer, S., and J. D. Jiménes Krause (Hhrg.), Dokumentationen und
Informationssysteme im Bereich pflanzengenetischer Ressourcen in Deutschland. Schriftenreihe zu
Genetischen Ressourcen (Zentralstelle für Agrardokumentation und -information - ZADI, Informationszentrum für Genetische Ressourcen - IGR), 64-79.
2. Hoppe, J. R. 1998: Practical suggestions for database implementations. In: Thiéry, M., Stevens, A.-D.,
Hoppe, J. R., Charles-Dominique, P., and Schuchmann, K.-L., Angiosperm pollination and seed dispersal,
a review. Ecotropica, 4, 69-91.
3. Hoppe, J. R. and Boos, E. 1996: Ein "Informationssystem Botanischer Gärten" auf der Basis des
Datenbanksystems SysTax. Gärtnerisch-Botanischer Brief, 124, 10-15.
4. Berendsohn, W. G. 1995: The concept of "potential taxa" in databases. Taxon, 44: 207-212.
187
Project ID: 01LC9902 (ARB)
1.4.2000 – 31.03.2003
AUTOMATED MAINTAINANCE OF INTEGRATED DATABASES FOR RRNA AND
OTHER RELEVANT GENE SEQUENCES; SOFTWARE DEVELOPMENT,
OPTIMIZATION, AND PARALLELIZATION FOR AUTOMATED SEARCHING OF
SEQUENCE IDENTIFIERS FOR ANALYZING DIVERSITY
W. Ludwig1 , R. Westram1 , L. Richter1 , H. Meier2 , and T. Ludwig3
1
Lehrstuhl für Mikrobiologie, Technische Universität München, am Hochanger 4, D-85350
Freising.
2
Lehrstuhl für Rechnertechnik und Rechnerorganisation, Technische Universität München,
Arcistr. 21, D-80333 München. 3 Institut für Informatik, Ruprecht-Karls-Universität Heidelberg,
Im Neuenheimer Feld 368, D-69120 Heidelberg
thomas.ludwig@informatik.uni-heidelberg.de; http://www. informatik.uni-heidelberg.de
ludwig@mikro.biologie.tu-muenchen.de; http://www. mikro.biologie.tu-muenchen.de
Key words: databases, identification, rRNA, specific probes, software development
Abstract:
Within the scope of BIOLOG the ARB project provides periodic releases of integrated databases
of processed sequence and other data for rRNA and other genes relevant in biodiversity studies.
New ARB software tools are currently being developed, optimized or evaluated for automated
database maintenance and evaluation. Further software developments focus on the automated
design and permanent in silico evaluation of comprehensive sets of taxon specific probes or
primers for biodiversity analyses according to the multiple probe concept. These probe sets will
later be accessible via the Internet.
Introduction. Comparative sequence analysis of informative genes nowadays plays a central role
in biodiversity studies for identification as well as phylogenetic and taxonomic analyses.
Doubtless, the currently most important marker genes are those encoding ribosomal RNAs
especially 16S/18S rRNAs. This is reflected by a dataset of about 30.000 primary structures
available in public databases. Given that the vast majority (> 90%) of prokaryotes cannot be kept
in pure culture thus far, comparative sequencing in combination with taxon specific probe and/or
PCR approaches often represent the only methods for identification, detection and quantification
in environmental samples [1]. These molecular methods are also getting more and more routine
techniques for studying diversity, evaluating taxonomy, identification and detection of eukaryotic
organisms.
The project. The most important prerequisites for diversity studies based upon these molecular
tools are comprehensive sequence databases and powerful software for database maintaining,
handling and analysis. Both aspects are in the focus of the ARB project initiated some years ago
[2, 3]. The ARB program package provides a software environment for these tasks. Processed
(with respect to alignment, quality, conservation, secondary structure and other criteria) sequence
data are stored in a central database. Any additional information such as descriptive data,
bibliography, user comments or links to external databases can be assigned to individual sequence
entries and visualized along with a phylogenetic tree. Database access and navigation is possible
via mouse click in that tree or by using search tools. A powerful editor for real or virtual primary
structures includes versatile tools for string searching, automated alignment and secondary
structure check as well as secondary structure visualization. Conservation profiles and column
filters can be established according to user defined criteria. Phylogenetic treeing can be performed
applying the respective integrated tools. A special maximum parsimony approach allows
188
reconstruction and optimisation of comprehensive trees representing the full sequence data set
(> 30.000 entries). The ARB PT server (positional tree) provides the basis for rapid searching of
closest relatives or specific sequence signatures. Such signatures can be evaluated as taxon
specific probes or primers against the background of the full database. The ARB package is
available at www.arb-home.de and in use worldwide.
Results. Within the scope of the BIOLOG project ARB databases comprising processed
sequences of small and large subunit rRNA, elongation and initiation factor, ATPase, RecA,
HSP60, polymerases and other conserved gene sequences including additional information and
analyses are maintained and permanently updated. Periodic releases are available at
http://www.arb-home.de or upon request.
In order to reduce the need of manpower and expertise for processing the rapidly increasing
amount of data, software for automation of database maintenance is being developed, evaluated,
and/or optimised. The focus is on further automation of data retrieval, selection, update or
correction, quality and higher order structure check, alignment, and phylogenetic analyses as well
as automated database optimisation based upon iterative cyclic application of the former tools.
The concept of integrated databases giving access to any data (molecular, physiological,
morphological, ecological and other) known for an organism has been followed from the
beginnings of the ARB project. Concerning the integration of foreign data ARB tools for rapid
access to external databases have been improved. Via mouse click and Internet browsers queries
can be performed according to ARB data field entries such as accession numbers, DNATAX
(project 01LC9904/2) numbers, organism names, or other identifiers on remote databases such as
EBI, GenBank, Medline, and SysTax. (project 01LC9901).
Another important task in ARB biodiversity informatics is the design and evaluation of taxon
specific probes or primers as tools for studies of diversity with the ultimate goal of automated
design and permanent evaluation of all potential probes against the background of the full data set
of available sequences. In the ARB version currently available, probe design is an interactive
procedure and can be performed for selected organisms or groups only. The ARB-PT-(positional
tree)-server which represents the central tool for probe design and in silico evaluation has been
improved. A new tool for automated definition of specificity levels according the criteria of
presence of potential target sites and phylogenetic relationship is currently being tested. Further
tools for finding all minimal probe sets characterizing individual taxa as well as for automated
designing comprehensive probe sets of hierarchical specificities according to the multiple probe
concept are currently being developed.
Software and database support is provided to the DNATAX project. Parts of the existing databases (rRNA elongation factor) can be immediately used, others (Cytochrome oxidase) have to be
established de novo. Collaboration with respect to data exchange and software development had
already been established between the ARB group and the BIOTA S04 project.
In collaboration with the MPI for marine microbiology and the VAAM (Vereinigung für
allgemeine und angewandte Mikrobiologie) four and two ARB courses for beginners (three days
each) and advanced users (two days each) have been performed.
References:
1. Amann, R. and Ludwig, W. 2000: Ribosomal RNA-targeted nucleic acid probes for studies in microbial
ecology. FEMS Microbiol. Rev., 24, 555-565.
2. Ludwig, W. and Strunk, O. ARB: a software environment for sequence data. http://www.arbhome.de/arb/documentation.html
3. Ludwig, W. 1995: Sequence databases. In: Akkermans, A. D. L., van Elsas, J. D., de Bruijn, F. J. (eds.),
Molecular microbial ecology manual, 3.3.5, 1-22. Dordrecht.
189
Project ID: 01LC9903 (ZEFOD)
01.05.2000 – 30.04.2003
REGISTER FOR BIOLOGICAL RESEARCH COLLECTIONS IN GERMANY
I. Brake 2 , W. Braun1 , D. Guttwein1 , I. Groß3 , F. Klingenstein3 , K-H. Lampe 2 , and S.
Roscher1
1
Zentralstelle für Agrardodumentation und –information (ZADI), Villichgasse 17, D-53177
Bonn. Roscher@zadi.de, http://www.zadi.de/igr/
2
Zoolog. Forschungsinstitut u. Museum Alexander Koenig, Adenauerallee 160, D-53113 Bonn.
i.brake.zfmk@uni-bonn.de, http://www.uni-bonn.de/museumkoenig/
3
Botanisches Institut u. Botanischer Garten Uni Bonn, Meckenheimer Allee 171, D-53115 Bonn.
biogart@uni-bonn.de, http://biogart@uni-bonn.de
Key words: access system, biodiversity, biodiversity informatics, biodiversity information,
biological collections, botanical gardens, botany, collection metadata, herbaria, zoological
museums, zoological gardens, zoology
Abstract:
Only occasional overviews and analyses of botanical and zoological research collections in
Germany exist at the moment, but individual collections are in various stages of self
documentation. The existing data will be combined by ZEFOD, systematized and, together with
new data, presented via the Internet as an expandable, interactive information network for a
broad range of users in science, administration, and the general public. Apart from an overview
of collections, the ZEFOD information system will provide a structural and content-orientated
description of biological research collections in Germany based on metadata provided by
collection holders. To record the metadata, questionnaires have been designed, mailed to the
collection holders, and made available via the ZEFOD homepage [1]. Metadata areas covered
include data on the institution or collection holder (address, contacts, staff), general information
about the collection (taxonomic and geographical foci, state of documentation), and a description
of the collection’s contents (number of objects, degree of computer recording, number of
nomenclatural types).
ZEFOD stands for “Zentralregister biologischer Forschungssammlungen in Deutschland”.
Current state of the database of national zoological collections. The questionnaires for
zoological collections in museums or universities were mailed in three batches (13.6., 3.8.,
5.9.2001) to 214 institutions. The questionnaires for zoological gardens have been mailed in
November, because these institutes usually have more time to do paper work in winter. By
October 9, 2001, 20% of the institutions had answered the questionnaires (42 institutions with 102
collections), 13% announced that they want to participate, but need more time, and 10% do not
hold the sort of collections required for participation in the ZEFOD database. In all 43% of the
institutions responded to our initiative within the first four months. A further increase of coverage
is expected, because the response rate for the first batch of questionnaires (mailed in June) has
reached 82%.
Some of the museums were visited in order to clarify the aims of the project and to obtain the
data. Similarly several scientific as well as other meetings within the community of collection
holders, curators, etc. were visited. Representatives of Naturweb (Austria) and BIODIV
(Belgium) were contacted in order to exchange experience with regard to online databases
carrying meta information about biological collections. A representative of ISIS (International
Species Information System), a database for zoological gardens, was contacted to check the
possibility of using some of their data for ZEFOD.
190
Current state of the database of national botanic collections. Questionnaires were mailed to 70
herbaria. For Botanic Gardens data from 435 special collections in 71 gardens have already been
collected in previous projects (see, e.g., [2]). The survey for living collections will be based on
this information, so the gardens will obtain partly filled-in questionnaires to complete their data in
a next step. An Access2000 database application is used for interim data collection and the data
existing for Botanical Gardens have been transformed into the ZEFOD data format and integrated
into the data bank. The database already accepts queries for different collection characteristics like
taxonomy, geography, biotopes, and functional types. It also allows a detailed analysis and shows
the amount, variety, and diversity of living plant collections in Germany and different spectra of
the representation of diversity in nature and in collections.
To ensure support for the project and to achieve maximum data quality, the Association of
Botanical Gardens (VBG) has been informed about the objectives and working plan of ZEFOD.
16 Botanic Gardens and 6 herbaria have already been visited to inform directors and staff about
ZEFOD and to give practical support in answering the questionnaire. Moreover, the project has
been presented in 4 meetings within the botanical collection community. Within this audience
ZEFOD is now well known and the utility of its aims is widely accepted.
Information technique. The ZEFOD database itself is being implemented as a relational
database, optimised to store the complex data resulting from the filled in questionnaires. Data
input from these questionnaires is done by routines reading the word files and by off-line input,
using interfaces containing ‘classical’ input fields (checkboxes, drop down lists, etc.). Tools for
online-data input are also under development which will help to develop ZEFOD into a
permanently sustainable information system. Acceptance of the Internet has to be improved,
however, before these tools will be used by all collection holders. Scripts for checking correctness
of links to homepages of the institutions are under development to guarantee that the provided
information is up-to-date.
One important access path to the information in ZEFOD is the search for taxa by their name.
Therefore a database structure to store scientific and common names is under development. The
user will also be supported by a thesaurus for querying system.
While data capture will be done through the whole phase of the project, some of the results are
already available by querying the online-database [1]. Technically this is realised by using
ORACLE 8i and scripts for creating dynamic pages. Access to institution names and places is free
but there is temporary a restricted area for accessing further details. This allows collection holders
to check the data before they will be published.
Biological collection information may be provided on the collection metadata level and on the
unit (specimen) level. ZEFOD provides metadata for all biological research collections in
Germany and will make unit level information access possible. Seen in the international context
ZEFOD is a direct contribution to international initiatives like the ‘Global Taxonomic Initiative
(GTI)’‚ the Convention on Biological Diversity (CBD) and the ‘Global Biodiversity Information
Facility (GBIF)’. ZEFOD is the German national node in BioCASE [3].
References:
1. ZEFOD Homepage. http://www.genres.de/zefod.
2. Rauer, G., v. den Driesch, M., Ibisch, P. L., Lobin, W., and Barthlott, W. 2000: Beitrag der deutschen
Botanischen Gärten zur Erhaltung der Biologischen Vielfalt und Genetischer Ressourcen. Bonn.
3. A Biodiversity Collection Access Service for Europe. http://www.biocase.org
191
Project ID: 01LC9904 ( EDIS)
1.4.2000 – 31.03.2003
EDIS – ENTOMOLOGICAL DATA INFORMATION SYSTEM
C. L. Häuser, J. Holstein, and A. Steiner
State Museum of Natural History, Rosenstein 1, D-70191 Stuttgart
chaeuser@gmx.de, holstein.smns@naturkundemuseum-bw.de,
steiner.smns@naturkundemuseum-bw.de; http://www.insects-online.de
Key words: biodiversity informatics, taxonomy, type specimens, world checklists, species
identification, DNA sequencing, molecular systematics, image analysis, geographical information
systems, bio-acoustics, interactive keys, insects, Collembola, Saltatoria, Lepidoptera,
Hymenoptera, Coleoptera, Acari, Araneae
Abstract:
The Entomological Data Information System (EDIS) is a co-operative research project jointly
undertaken by a number of leading German natural history museums and university institutions in
the area of biodiversity informatics. The purpose of EDIS is to facilitate access to information
about insects and other arthropods for biodiversity research and the general public by developing
and linking relevant databases and information tools established by EDIS partner institutions.
The information provided by EDIS includes scientific data about insects from a wide variety of
sources ranging from global species inventories, molecular sequences, digital images and sounds
to geographic distribution maps.
EDIS organisation:
The EDIS framework project is co-ordinated at the State Museum of Natural History, Stuttgart,
Germany, and currently comprises six separate projects (ABIS, DNA-TAX with sub-project
MOLART, DORSA, GART, INGE and OBIF) undertaken by different partner institutions. The
databases and information systems developed by EDIS are in the public domain and will all be
accessible via a central Internet gateway developed in co-operation with the Department of
Systematic Botany and Ecology at the University of Ulm, Germany, making use of the SysTax
database system. The individual EDIS projects are implemented independently by the respective
research institutions, and focus on specific questions in selected insect groups (see following
status reports of individual projects). The information provided by EDIS is primarily intended for
use by research and development, but also for other users with a general interest in insects.
EDIS goals:
• establishing and extending scientific databases on insects using collection specimens,
literature, multimedial and molecular data
• developing and improving new IT-tools for identification of - and information access on insects
• linking separate databases containing insect biodiversity data of various kinds through a
common access point
• providing a common public gateway to a variety of information sources about insects
192
193
Project ID: 01LC9904 (EDIS, Subproject ABIS)
1.4.2000 – 31.03.2003
ABIS - AUTOMATED IDENTIFICATION OF BEE SPECIES
V. Steinhage 2 , T. Arbuckle 2 , S. Schröder1 , A. B. Cremers 2 , and D. Wittmann1
1
Institut für Landwirtschaftliche Zoologie und Bienenkunde, University of Bonn, Melbweg 42,
D-53127 Bonn
2
Institut für Informatik III, University of Bonn, Römerstr. 164, D-53117 Bonn
steinhag@cs.uni-bonn.de
Key words: species identification, taxonomy, image analysis, supervised learning, biodiversity
informatics
Abstract:
Computer aided systems have become popular in zoological systematics in recent years. For
insects, the difficult taxonomy and the lack of experts greatly hamper studies on conservation and
ecology. Bees in particular, as the main pollinators of food crops, represent a critical ecological
and economical resource which needs to be carefully exploited and managed. We have developed
a system for the automated identification of species that employs knowledge-based image analysis
of the bee forewings. The system has been successfully deployed in Germany, Brazil and the U.S.
Results:
The key idea of the Automated Bee Identification System ABIS is to identify bee species from
images of their forewings. Using diffuse background illumination, the wings of the bees show a
clear venation within a transparent skin. The structure of this venation is genetically fixed and
therefore suited to species identification [1]. Analysing each wing image gives rise to a set of
about 300 characteristic numerical features. These features are used in a new knowledge-based
classification approach to identify the bees’ species. The image analysis and classification are
performed in a hierarchy of consecutive operations. First, ABIS identifies the genus. In the second
step, ABIS identifies species groups – so-called species complexes. In the final step, ABIS
employs a new non-linear discriminant analysis to perform the species identification [2]. Each
step of this hierarchical approach is guided by the knowledge extracted at the previous step [3].
The result of this classification can be visualised in terms of cluster images or dendrograms. The
equipment required is easily portable and suited for work in collections or in the field [4].
Fig. 1: Use of ABIS in collections
Fig. 2: Automatic image analysis
Fig. 3: Use of ABIS in the field
We give two representative results of our prototype system. Figures 4 and 5 show species
identifications for the genera Bombus and Colletes. Figure 4 shows the genus identification with
two clearly separated cluster centres (top: the Bombus species, bottom: the Colletes species).
Figure 5 shows the identification of two species complexes of the genus Bombus. The seven
Bombus species (B. cryptarum (Fabr. 1775), B. lucorum (L. 1761), B. magnus Vogt 1911, B.
terrestris, B. ruderarius, B. sylvarum und B. veteranus) in the given example belong to two
194
subgenera: Bombus (Bombus) and Bombus (Thoracobombus). The clustering in figure 5 and its
visualisation in figure 6 clearly show the systematic difference in species membership. For the
identification of the species, we employed a new nonlinear discriminant analysis technique and
were able to identify even the very difficult to distinguish species Bombus sylvarum and Bombus
veteranus with a correct classification rate of 99.3 % using a leave-one-out cross-validation
procedure.
Fig. 4: Genus identification
Fig. 5: Identification of species
complexes
Fig. 6 Visualisation by means of
a Dendrogram
ABIS has been successfully applied to species identification in Germany, Brazil and the U.S.
Furthermore ABIS can be applied to other insects such as wasps or flies. The ABIS project is an
important and innovative part of the joint EDIS project which aims to build and establish a
national Entomological Data Information System in Germany. EDIS is funded by the Federal
Ministry of Education and Research as a part of the programme Biodiversity and Global Change
(BIOLOG). EDIS will permit Internet access and will comply with international standards. It
should therefore play a role as a national centre for mapping and monitoring and as the basis for
international exchange of information concerning the biodiversity of insects.
The important and crucial first step for each information system is the capture and classification of
input data. ABIS offers an efficient solution of this task within the field of mapping and
monitoring of insect species.
References:
1. Schröder, S., Wittmann, D., Roth, V., and Steinhage, V. 1998: Automated Identification System for
Bees. Proc. XIII Intern. Conf. of IUSSI, Dec. 29, 1998 - Jan. 3, 1999, 427, Adelaide/Autralia.
2. Roth, V. and Steinhage, V. 1999: Nonlinear Discriminant Analysis Using Kernel Functions. In: 13th
Internat. Conf. on Neural Inform. Processing Systems, NIPS `99, Nov. 30 – Dec. 2 1999, Denver.
3. Roth, V., Pogoda, A., Schröder, S., and Steinhage, V. 1999: Pattern Recognition Combining Featureand Pixel-based Classification within a Real World Application. In: Mustererkennung 1999, 21. DAGMSymposium, Informatik aktuell, 120-129, Bonn.
4. Arbuckle, T., Schröder, S., Steinhage, V., and Wittmann, D. (in press): Biodiversity Informatics in
Action: Identification and Monitoring of Bee Species using ABIS. Proceedings of Sustainability in the
Information Society, Conf. on Environmental Informatics, Oct. 10 - 12 2001, ETH Zürich.
195
Project ID: 01LC9904 (EDIS, Subproject DNATAX 1)
1.7.2000 – 31.3.2003
DNATAX – DNA TAXONOMY AS A TOOL IN BIODIVERSITY RESEARCH:
RECORDING, ASSESSMENT, MONITORING AND UTILIZATION OF THE
GENETIC DIVERSITY OF EUKARYONT SYSTEMS
A. Hille, M. A. Miller, and G. Haszprunar
Zoologische Staatssammlung München, Münchhausenstr. 21, D-81247 München
axel.hille@zsm.mwn.de; http://www.zsm.mwn.de/wiss_start.htm
Key words: insects, DNA sequencing, taxonomy, molecular systematics, database, biodiversity
informatics
Abstract:
The DNATAX project aims at compiling diagnostic DNA signatures for the main orders of insects.
It focuses on the elaboration of phylogenetically informative DNA sequences (e. g. for Geometrid
moths, INGE). Data are organized and streamlined in a database on the World Wide Web
(SysTax, Ulm) for more efficient use in taxonomic and systematic basic research.
Introduction. In species-rich groups like the insects, which are represented in the environment
and in collections by millions of specimens and tenthousands of species, accuracy of identification
often is a serious problem for taxonomists and even more for the non-specialist scientists in
applied zoology, especially dealing with very small species or immature stages of insects (eggs
and various kinds of larvae). In addition, accurate phylogenetic trees provide a sound basis for
many questions of evolutionary biology concerning species discrimination and speciation,
biogeographic and ancestry-descendance relationships, but also concern important applied aspects
like pest control and bio-indication. DNA-Taxonomy provides tools to cover both – identification
and phylogeny.
In the initiation phase of the project a complete molecular lab including an ABI377 automated
sequencer was established at the Zoologische Staatssammlung München (ZSM), an important
institution holding natural history collections. The laboratory devices are intended for highthroughput sequencing which allows operating on different molecular markers such as
mitochondrial (mt) or nuclear (c) DNA gene fragments. The broader relevance of molecular-aided
taxonomy is to obtain clearly defined taxa. Extensive DNA surveys of species limits and
geographic lineage distribution, e. g. delimiting taxonomic units in species complexes are targeted
by dense taxon sampling of morphologically highly variable geographic populations. A second
important aim is to solve long pending problems in taxonomic classification, systematics, and
phylogeny.
Results. At the start of the project protocols for DNA extractions from fresh and archival
biomaterials were tested and optimised for routine DNA typing. Genomic DNA (over 600 probes)
was isolated from either single legs or plugs taken from the thorax of single dried insects, or from
dead animals recovered from the field (pheromone traps, ethanol-fixed fresh specimens). To link
an individual to a source (a voucher, frozen biomaterial like tissue) by DNA typing, the repository
of extracted DNA and its sources are curated in a resource bank at the ZSM, which allows
applications for studying genetic variation, phylogeny, and the processes underlying diversity,
such as gene flow. Laser-scanned label data on these repositories and annotation information on
the voucher specimen can simply be exported in tabular form to secondary composite databases
like SysTax using controlled vocabulary.
Since recommendations made by Caterino et al. [1] outline that “the cytochrome oxidase I, 16S,
18S, and elongation factor -1∀.[...] are informative across a broad range of divergences in insects
196
[..]”, PCR-amplified fragments of these genes (more than 1200 contigs) are also used in our
sequence efforts to identify species and lineages. These genes provide potent discriminators of
phylogenetic relationships for newly determined sequences for insects, and are a valuable asset to
the prospect of a global comparability with the substantial database that already exists for these
genes in the principal DNA sequence databases like NCBI-GenBank/EMBL-EBI. In order to
exploit the folding structure of RNA molecules in an approach called “molecular morphometry”,
the 16S like mitochondrial ribosomal RNA is now also routinely sequenced for the studied taxa
(100 alignable sequences). Sequences are stored in ARB (see this volume), and compared to its
structural alignments preheld in the “pt_Server” to recognize sequence and fold patterns using one
of the secondary structure models of the SSU rRNA. Several sequencing projects are elaborated
so far focussing on taxon description [2], identification of critical species [3], test of conspecifity
of allopatric populations [4], and molecular systematics [5]. Others are under way by cooperation
with national (Museum Koenig, Bonn, Lepidoptera: genus Zygaena, [6]; ZSM, Lepidoptera:
Geometridae, Drepanidae, Diptera: Diopsidae, Hymenoptera: genus Euglossa; University of
Jena, [7], University of Bremen, MOLART) and international partners (Zoological Museum, Dep.
Entomol., Copenhagen, Diptera: Sepsidae, and genus Themira).
Submission of sequence data and associated biological information to the EMBL database at EBI
using the WWW site WEBIN is a standard practice prior to publications [2-5]. The unique
accession number assigned by the database which permanently identifies the sequence submitted,
is cross linked by queries made by SysTax. This interoperability using compressed versions of
GenBank’s feature table flat-file format ensures availability and distribution of new sequence
data.
Phylogenetic trees as graphical representations of the putative evolutionary relationship between
groups of organisms, e.g. as calculated from multiple nucleic acid sequence alignment can be
generated by the specialized treeing programme tools of ARB. Using its tree-building functions
according to an optimised maximum parsimony criterion, homologies between novel query
sequences (i. e. of a newly sequenced animal) and stored database alignments are identified by a
searching strategy which finds similar alignment positions in local sub trees. Nearest neighbour
terminals of localized sub trees may be interchanged during the optimisation fit, while the global
tree topology is maintained.
References:
1. Caterino, M. S., Soowon Cho, and Sperling, F. A. H. 2000: The current state of Insect Molecular
Systematics: A thriving tower of Babel. Annu. Rev. Entomol., 45, 1-45.
2. Buchsbaum, U. and Miller, M. A. (in prep.): Cerace diehli sp. n. from Sumatra (Indonesia) (Lepidoptera,
Tortricidae, Tortricinae). Heterocera Sumatrana.
3. Hille, A. and Miller, M. A. (in prep.): DNA sequence variation at the mitochondrial cytochrome oxidase
I subunit among pheromotypes of the sibling taxa Diachrysia chrysitis (LINNAEUS, 1758) and D. tutti
(KOSTROWICKI, 1961) (Lepidoptera: Noctuidae).
4. Spies, M., Sublette, J. E., Sublette, M. F., Wülker, W. F., Martin, J., Hille, A., and Miller, M. A. (in
print): Pan-American Chironomus calligraphus Goeldi (Diptera, Chironomidae): species or complex?
Evidence from external morphology, karyology and DNA sequencing. Aquatic Insects, 24(4).
5. Miller, M., Hausmann, A., and Trusch, R. 2001: The phylogenetic relationships in Geometrid moths. An
approach using mitochondrial DNA (mtDNA) sequences. Spixiana, 24 (3), in print.
6. Hille, A., Miller, M. A., Edelmann, A., and Naumann, C. M. (in prep.): Biochemical and Molecular
Systematics of selected taxa of the genus Zygaena (Zygaenidae, Lep.).
7. Erlacher, S., Trusch, R., Hille, A., and Miller, M. A. (in prep.): Phylogeny of the geometrid moth taxon
Dyscia inferred from morphological data and nucleotide sequences of the mitochondrial partial ND1, 16S
and COXI genes (Lepidoptera, Geometridae).
197
Project ID: 01LC9904 (EDIS, Subproject DNATAX 2 - MOLART)
1.4.2000 – 31.3.2003 1
MOLART (DNATAX 2): MOLECULAR IDENTIFICATION OF COLLEMBOLA:
METHOD OPTIMISATION VERIFICATION - DOCUMENTATION
J. Filser1 , U. Burkhardt 1 , and A. Fjellberg 2
1
University of Bremen, Universität Bremen, UFT - Abt. für allgemeine und theoretische
Ökologie, Leobener Str., D-28334 Bremen
2
Entomological Research, Ormeletveien 53, 3145 Tjoeme, Norway
filser@uni-bremen.de
Key words : Collembola, Nordic species, biogeography, Protaphorura armata group, Isotoma
viridis group, DNA, molecular methods
Abstract:
MOLART deals with the development and standardisation of molecular methods for safe and
efficient identification of species which cannot (or only with great effort and expertise) be
distinguished by morphological analysis. Different molecular techniques (such as DNA
sequencing, AFLP, RAPD, allozymes) are applied, refined and evaluated according to their
diagnostic power, their efficiency for different taxa, the necessary effort of time and the
required experience This is exemplified by two problematic species groups within the
Collembola (Insecta, Apterygota), the Protaphorura armata group and the Isotoma viridis
group. To ensure precise identification, the project is conducted in close cooperation with an
international expert in Collembola taxonomy - Arne Fjellberg - who will also provide
specimens of further Collembola groups for DNA-Tax 1, a database of his collection on
Nordic Collembola and volume 2 of his identification key for Collembola of Fennoscandia
and Denmark. At present, 9 genera of the family Isotomidae have been finished. For
molecular analyses, a number of sites have been sampled, the first laboratory cultures have
been started, and the DNA techniques were established. First analyses revealed a) differences
within three species of the I. viridis group, b) restriction enzymes that could be used for quick
species distinction, and c) that the power of the methods used so far differs between the two
species groups investigated.
Results:
1. Identification
The first nine months of the project have mainly dealt with extending and updating the
identification key (monograph "The Collembola of Fennoscandia and Denmark"), building up
laboratory infrastructure, establishing cooperations, collecting animals and establishing
cultures.
Concerning the identification key, 9 genera of the family Isotomidae have been finished (incl.
one n.sp. of Folsomia). The rest of the family (incl. 3 n.sp.) will be finished by the end of next
year, together with the family Entomobryidae. The work for the remaining families of the
Entomobryomorpha and Symphypleona will be conducted in 2003. The final Vol. 2 of the
monography is expected to contain a total number of 223 species.
Representatives of the genus Protaphorura and the Isotoma viridis group have been collected
from 14 sites in Germany, Denmark and Norway. The live catches are now being used for
establishing laboratory cultures to obtain sufficient animal numbers for molecular biological
1
The project started only in January 2001
198
analyses. Thus far, DNA has been extracted, amplified and purified mostly from I. viridis s.l.
(whole individuals, except for the furca which has been preserved for taxonomic check).
Studies with four primer pairs out of subunits of the mitochondrial cytochrome oxidase gene
(CO1-fa, CO1-ru, CO2-ab, CO3-ab) revealed CO2-ab to be most appropriate for I. viridis s.l.,
CO3-ab for Protaphorura sp.. The first individuals have been sequenced; new results are
produced continuously. Based on them, appropriate restriction sites have been selected which
can be used for displaying haplotype-specific length polymorphisms on agarose gels: a first
step towards easy, quick, affordable and safe molecular identification (Fig. 1)
Present technique
1.
2.
3.
4.
5.
6.
7.
Field collection
Microscopic identification
Establishment of laboratory cultures
Separation of furca --> (2)
DNA extraction, amplification, purification
Sequencing
Identification of haplotype-specific restriction
sites
8. Design of specific restriction enzymes
9. Specific fragment length polymorphism on
agarose gels
Future technique: easy and quick
identification
1. Field collection
5. DNA extraction, amplification,
purification
9. Specific fragment length
polymorphism on agarose gels
Fig. 1: An example for improving molecular identification of Collembola within MOLART
2. Interoperability and Linking of Databases
The "collection Fjellberg" comprises about 13,000 records of Nordic Collembola, all of them
including locality, habitat, date, collector, literature source and synonyms. The data are ready
for import to Systax.
3. Life in time and space: collections as biodiversity information resources
MOLART is specialised in Collembola (Insecta, Apterygota), with special respect to the
Nordic fauna. Here, one of the most complete collections worldwide is the "collection
Fjellberg" which will be provided as a database by this project.
References:
1. Filser, J. 1999: Habitat requirements and ecology of Isotoma viridis Bourlet, 1839 and Isotoma
anglicana Lubbock, 1862 (Insecta, Collembola). Braunschweiger Naturkundliche Schriften, 5/4, 905-911.
2. Fjellberg, A. 1998: The Collembola of Fennoscandia and Denmark. Part I: Poduromorpha. Fauna
Entomologica Scandinavica 35, Brill, Leiden, Boston, Köln, 184pp.
3. Frati, F. & Carapelli, A. 1999. An assessment of the value of nuclear and mitochondrial genes in
elucidating the origin and evolution of Isotoma klovstadi Carpenter (Insecta, Collembola). Antarctic
Science 11: 160-174.
4. Simonsen, V., Filser, J., Krogh, P.H. & Fjellberg, A. 1999. Three species of Isotoma (Collembola,
Isotomidae) based on morphology, isozymes and ecology. Zoologica Scripta 28, 281-287.
5. Zabeau, M. & Vos, P. 1993. Selective Restriction Fragment Amplification: A general method for DNA
fingerprinting. European Patent Application EP 0534858.
199
Project ID: 01LC9904 (EDIS, Subproject DORSA)
1.4.2000 – 31.3.2003
DORSA - GERMAN ORTHOPTERA COLLECTIONS
S. Ingrisch, K.-H. Lampe , K. Riede , and C. Dietrich
Zoologisches Forschungsinstitut & Museum Alexander Koenig (ZFMK),
Adenauerallee 150-164, D-53113 Bonn
sigfrid.ingrisch@planet-interkom.de; http://www.dorsa.de
Key words: specimen database, geographical information systems, bio-acoustics, automated song
recognition, documentation of type specimens
Abstract:
The DORSA subproject (abbreviation for "Deutsche Orthopteren-Sammlungen" = German
Orthoptera Collections) is a specimen based Internet database of the Orthoptera held in German
museum collections including illustrations of type specimens, sound recordings, and geographic
information on a world-wide basis ("virtual museum"). A rapid assessment tool is developed for
automated song recognition.
Results:
The aim of DORSA is to provide web access to (i) specimen information of Orthoptera in
German collections, (ii) a virtual Orthoptera collection with link to the Orthoptera Species File
Online [1], (iii) a photographic documentation of type specimens and other important specimens,
and (iv) the wealth of acoustic data of Orthoptera in German institutions and private collections.
Geo-referencing and combining the locality data with a geographical information system will be
tackled further on.
Specimens databased up to now include type specimens (holo-, syn- and paratypes; see table 1), a
selection of specimens on which important monographs were based, and voucher specimens for
which sound recordings are available. The project covers collections held in the following
museums: Berlin (ZMHB), Bonn (ZFMK), Dresden (SMTD), Eberswalde (DEI), Frankfurt
(SMFD), Halle (MLUH), Hamburg (ZSMH), München (ZSM), and Stuttgart (SMNS). The
private collections of Heller, Ingrisch, Riede and G.H. Schmidt will also be included. Databasing
of specimens has been completed in the museums of Dresden, Eberswalde, and Hamburg, started
but not yet completed in the other collections. The current state is documented in table 1. To trace
the current locality for a given type specimen is often difficult due to incompletely published
information, insufficient labelling of specimens, exchange between museums, destruction of
specimens (i.e. in war), or "prolonged" loans. DORSA helps to improve the knowledge of the
current type depositories. Information on the primary types of 424 species could so far be updated
as compared with the Orthoptera Species File (OSF). From the taxonomic point of view, the
focus was so far on the Tetrigoidea (354 species, 1014 specimens), Gryllacridoidea (257 [331
with Halle, Stuttgart and Munich included] species, 467 [593] specimens), and Grylloidea (323
[341 with Stuttgart and Munich included] species, 619 [655] specimens). The inventory data for
those three groups are ready for import into the all-biology database SysTax.
12,000 photographs have so far been taken to document the specimens and the labelling of the
specimens; about half of the photographs are already processed for web publishing.
For all specimens with reliable locality information, collection sites are geo-referenced by
latitude/longitude co-ordinates. This allows mapping by any geographical information system
(GIS) and many additional applications, such as intersection with environmental data. A first
prototype for a Java-based graphical user interface can be found at www.groms.de. It allows
200
geographic queries, retrieval and mapping of species data. Modern collections may be reliably
geo-referenced in the field by means of a GPS. However, for information stored on historic
specimen labels co-ordinates have to be looked up, using atlases or gazetteers. So far more than
2,000 localities have been reliably geo-referenced with latitude/longitude co-ordinates.
Tab. 1: Recorded (roman font) and estimated (italics) type specimens of Orthoptera in German museum
collections.
museum
Berlin
Eberswalde
Dresden
Hamburg
Halle
Bonn
Frankfurt
Stuttgart
München
Sum
taxa with taxa with taxa with number of
total
total type data in primary primary
no taxon
primary primary
types
type recorded recorded OSF not types lost types not entry found
types in
types including specimens species specimens confirmed
(taxa) listed in
in OSF
OSF checked paratypes
in museum
OSF
1115
1546
2061
4915
888
2004
15
141
68
47
72
141
410
164
447
1
25
66
118
168
521
294
905
6
57
9
144
130
200
367
253
478
5
57
47
39
54
72
172
1
8
6
8
11
26
82
135
183
330
173
364
39
30
47
65
87
207
27
42
68
199
1
1573
2171
2991
7148
1772
4198
27
59
317
107
unlabelled,
newly
recognised
prim. types
11
2
13
The sound library comprises so far 4,000 sound recordings. The species-specific songs are used as
a knowledge base for song recognition algorithms based on neural networks. First results indicate
that reliable automated classification is possible for songs of Grylloidea from South East Asia and
Amazonia. Automatic song recognition in crickets (Grylloidea) and katydids (Tettigonioidea) is
a 4-step process: (1) pre-processing of the raw time signals, (2) segmentation of the filtered
signals into single pulses, (3) extraction of typical characters (called features in the pattern
recognition literature) of pulses or pulse series, (4) automated fusion and classification. In the
pre-processing stage sound recordings are filtered to lessen environmental noise, making them
better suited for the segmentation task. During signal segmentation single pulses are extracted
from the filtered time series based on short-time energy analysis. In the feature extraction global
and local features are extracted from the signal. Global features are characterising complete songs
of an individual, local features are characteristic for single pulses or short segments of the signal.
Characters most suitable for classification of crickets are the distance between pulses, the duration
of pulses, and the frequency course within pulses. The feature vectors are classified individually
and the results of the single classifiers are combined through classifier fusion. This leads to local
class decisions which are based on the whole feature set. These local class decisions are combined
through temporal fusion over the whole time series. The classification uses artificial neuronal
networks and statistical procedures, e.g. Radial Basisfunction Networks (RBF), Multi-LayerPerceptrons (MLP), and Fuzzy-K-Nearest-Neighbour (Fuzzy-K-NN). The feature extraction and
analysis tools are written in Matlab. Feature evaluation of single characters and sets of characters
is important to find the optimal feature combination. The importance of single characters depends
on species and individuals. For successfully training the neural networks we need a dataset from
known taxa that allows to adapt the neuronal architecture. For that we need sound recordings from
as many as possible different individuals of the same species. At present, the tool for the
automatic sound recognition runs properly with a set of 215 songs from 137 individuals and 30
cricket species.
References:
1. Otte and Naskrecki 1997: OSF. http://viceroy.eeb.uconn.edu/Orthoptera
201
Project ID: 01LC9904 (EDIS, Subproject GART)
1.4.2000 – 31.03.2003
GART – GLOBAL SPECIES REGISTER BUTTERFLIES
C. L. Häuser, J. Holstein, and A. Steiner
State Museum of Natural History, Rosenstein 1, D-70191 Stuttgart
chaeuser@gmx.de, holstein.smns@naturkundemuseum-bw.de,
steiner.smns@naturkundemuseum-bw.de; http://www.insects-online.de/projfram.htm
Key words: biodiversity informatics, taxonomic databases, type specimens, world checklist,
Lepidoptera, Papilionoidea, Papilionidae, Pieridae
Abstract:
The GART project’s aim is to provide general access to information about the world’s butterflies
based on global taxonomic catalogues. In a first step, global species registers were developed for
two families of butterflies, Swallowtails (Papilionidae) and Whites (Pieridae), based on
comprehensive taxonomic databases. In addition to extending the taxonomic database, all
butterfly primary type specimens housed in the collections of the co-operating GART partner
institutions will be recorded, photographed, and databased. For selected taxa within the
Papilionidae and Pieridae, a comprehensive species information system will be developed
offering data about distribution, ecology, and morphology of individual species as well as links to
other existing data sources. The species register and the underlying taxonomic and type specimen
databases will be made publicly available through a central user interface on the Internet via the
EDIS framework project.
Results:
The GART project is developing a worldwide information system for butterflies (Papilionoidea).
It is based on taxonomic and bibliographic databases, which will contain information on all
described butterfly taxa, including scans of the original descriptions, illustrations, and on
photographs of the primary type specimens in institutional collections.
GART is jointly undertaken by partners from eight major German natural history museums:
Staatliches Museum für Naturkunde (SMNK), Karlsruhe; Deutsches Entomologisches Institut
(DEI), Eberswalde; Zoologische Staatssammlung München (ZSM); Museum für Naturkunde der
Humboldt Universität (MNHU), Berlin; Forschungsinstitut und Naturmuseum Senckenberg
(FIS), Frankfurt; Staatliche Naturhistorische Sammlungen Dresden, Museum für Tierkunde
(SMTD); Zoologisches Forschungsinstitut und Museum Alexander Koenig (ZFMK), Bonn, and
is co-ordinated at the State Museum of Natural History, Stuttgart (SMNS). The project is also
intended to provide a distinct contribution to the international 'Global Butterfly Information
System' (GloBIS) initiative, to be developed jointly by SMNS and several international research
collections [1-2].
In collaboration with leading butterfly taxonomists GART has developed annotated global
checklists of the families Papilionidae and Pieridae, which include all currently recognized
species names and important synonyms. These lists are already available via the WWW and will
be periodically updated and maintained by a group of international specialists [3].
The GART taxonomic database includes information on the original description, primary and
recent taxonomic status, taxonomic history, type locality (both original and subsequent
information), type status as well as primary type material (including condition and current
depository). Each record is linked to a literature database and to specimen and label photographs.
202
To date, the databases contain more than 1,000 taxon records, more than 20,100 literature
references, and over 2,500 digital specimen images.
These data will regularly be transferred to the SysTax-database system at the University of Ulm
[4], where they will be made publicly accessible via the Internet in combination with data from
other EDIS projects.
Prior to the establishment of the GART databases, general standards for recording taxonomic
data were formulated and agreed, which have also been adopted by other EDIS projects (INGE,
OBIF). For the digital documentation of type specimens, special equipment (lighting devices)
and procedures have been developed to ensure uniform and durable results.
For the difficult and time-consuming task of identifying previously unrecognised type specimens
from historic collections, GART is being supported by several renowned butterfly specialists
(Drs. Y. Nekrutenko, G. Lamas, and V. Lukhtanov) who have been working in the museums of
Berlin, Dresden, and Stuttgart.
Fig. 1: An example for a GART database entry: Comparison between original
description (with illustration), and preserved type specimens (with labels).
References:
1. Lamas, G., Nielsen, E. S., Robbins, R. K., Häuser, C. L., and de Jong, R. 2000: Developing and sharing
data globally: the 'Global Butterfly' Information System' - GloBIS. In: Gazzoni, D. L. (ed), XXIth
International Congress of Entomology, Foz do Iguassu 2000, Abstracts, 1. Embrapa Soja, Londrina,
Parana, 196.
2. http://www.ento.csiro.au/globis/
3. Häuser, C. L., Holstein, J., and Steiner, A. 2001: Homepage of the Global Species Register Butterflies,
http://www.insects-online.de/gartfron.htm
4. http://www.biologie.uni-ulm.de/systax/index.html
5. Häuser, C. L. 2001: Papilionidae – revised GloBIS/GART species checklist (2nd draft),
http://www.insects-online.de/frames/papilio.htm
203
Project ID: 01LC9904 (EDIS, Subproject INGE)
1.4.2000 – 31.3.2003
INGE – INVENTORY OF GEOMETRID MOTHS OF EUROPE (INSECTA,
LEPIDOPTERA): PROVIDING MULTIMEDIAL DATA ON SYSTEMATICS,
ZOOGEOGRAPHY AND ECOLOGY
A. Hausmann and R. Trusch
Zoologische Staatssammlung München, Münchhausenstr. 21, D-81247 München
Axel.Hausmann@zsm.mwn.de; http://www.zsm.mwn.de/Lepidoptera/Biolog/index.htm
Key words: Geometridae, Europe, systematics, zoogeography, ecology, multimedial publication
Abstract:
The main purposes of the INGE project are the critical examination and the coordinated
compilation of available information on systematics, zoogeography, and ecology of the European
Geometrid Moth species (Lepidoptera). The data are published using different media, such as a
book series (Apollo Books, Stenstrup, Vol. 1 published in 3/2001), CD-ROM (Expert-center for
Taxonomic Identification, ETI, Amsterdam, data partially submitted) and the Internet (SysTax,
Ulm, data partially submitted). The results will offer easily accessible identification tools and
biodiversity databases which include a virtual type collection and will greatly reduce the need of
searching for material scattered widely in many institutions. Geometrids being one of the largest
and worldwide distributed insect groups, INGE can serve as a model for modern methods and
achievements in measuring and conserving biodiversity.
Results:
In the first half of the project period collection-based biodiversity data have been compiled to gain
the information needed on morphology, distribution patterns and bionomics of European
Geometridae (Archiearinae-Sterrhinae). The Zoologische Staatssammlung München (ZSM)
houses one of the world’s largest collections of moths including about 200,000 specimens of the
subfamilies focused on by the project. Almost half of them have been investigated so far.
Microscopic slides of about 3,000 dissected specimens served as the base for morphological
studies and for 130 line drawings. Additionally, material from most major European collections
was examined (about 100,000 specimens). Distribution maps reflecting collection data have been
created for 120 species (of 240).
Quality control of all taxonomic information is one of the crucial aims of this monographic
project. In this respect it overlaps with the EDIS–GART project, which established high standards
in data-collecting and data-processing. Applying those standards, original descriptions of more
than 1,000 taxa have been checked and largely databased. Interdisciplinary interaction with the
DNA-TAX projects helps to evaluate the status of taxa at the species level (e.g. validity at species
rank of Timandra comae has been confirmed), as well as on generic level (e.g. verification of
sistergroup-relationship of the tribes Cosymbiini and Timandrini; synonymy of Glossotrophia and
Scopula) [1]. To date, DNA-information has been provided for about 200 specimens of
Geometridae.
The first volume of the series „The Geometrid Moths of Europe“ [2] has already been published
(282 pp.). The project is proceeding regularly and both Vol. 2 and the interactive CD-ROMs (by
ETI) will be published in 2003, as planned.
Taxon-based data (checklists) from these publications are transferred via the GART-database into
SysTax, where they will be made accessible via the Internet. Basic taxonomic information on the
species under focus has already been submitted to SysTax. Furthermore, an object-database
204
contains photos and object (label) data of primary type specimens and other characteristic
individuals of all 240 European species of the subfamilies Geometrinae and Sterrhinae. 340
specimens are databased this way (partially submitted to SysTax).
Interactions and co-operations: The INGE project is a core activity crucial for other research.
Alterations of feeding behaviour of certain Lepidoptera (e.g. Cameraria ohridella, Gracillariidae)
can cause dramatic problems. DNA analysis of populations or subspecies with regional host-plant
preferences may provide a basis for the prevention and biological control of pests [3].
Prerequisites are exact knowledge of regional host-plant utilisation linked with a secure
taxonomy, combined with molecular genetic characterisation of properly determined specimens.
In this respect INGE can benefit from the international project Geometrid Moths of Europe, the
Research Initiative Herbulot [4], and the facilities of the Molecular Research Laboratory at the
ZSM, a rather unique constellation in Germany.
Fig. 1: Information flow and publication activities in the INGE project
References:
1. Miller, M., Hausmann, A., and Trusch, R. 2001: The phylogenetic relationships in Geometrid moths. An
approach using mitochondrial DNA (mtDNA) sequences. Spixiana, 24 (3), in print.
2. Hausmann, A., 2001: Introduction. Archiearinae, Orthostixinae, Desmobathrinae, Alsophilinae,
Geometrinae. In: A. Hausmann (ed.), The Geometrid Moths of Europe, 1, 1-282.
3. Hausmann, A. und Trusch, R. (in print): Proceedings of the FORUM HERBULOT 2001. Spixiana 24.
4. http://www.herbulot.de
205
Project ID: 01LC9904 (EDIS, Subproject OBIF)
1.4.2000 – 31.03.2003
OBIF - OPTIMIZATION OF BIODIVERSITY INFORMATION FACILITIES ON
APPLICATION-ORIENTED RESEARCH
M. Verhaagh, J. Spelda, C. Wurst, L. Beck, N. Blüthgen, F. Brechtel, W. Hanagarth, H.
Höfer, F. Meyer, and S. Woas
State Museum of Natural History Karlsruhe (SMNK), Erbprinzenstr.13, D-76133 Karlsruhe
verhaagh_smnk@compuserve.com; http://www.naturkundemuseum-karlsruhe.de/obif.htm
Key words: biodiversity information, natural history museum collections, digitalisation,
interactive key, Oribatida, Araneae, Formicidae, Buprestidae
Abstract:
The OBIF project aims at optimising the input of - and access to - biodiversity data from research
projects and collections at the State Museum of Natural History Karlsruhe. It combines electronic
identification keys and morphological characters, information on collections sites and
distribution of species, ecological data, text information, references with line drawings, digital
light microscopy and REM-photos, as well as video sequences into an easy-to-use information
system for the scientific and public user on the Internet and in the museum.
Introduction
Natural History Museums are traditionally devoted to recording and describing, i.e. inventorying,
the biological diversity on earth. Therefore, they store and administer extensive scientific
collections and distribute knowledge on natural history to the public.
OBIF has the objective to optimise the in- and output of biodiversity data at the State Museum of
Natural History Karlsruhe. Scientists and technical assistants need the data internally for research
and for the administration of the collection. On the other hand, biodiversity data is also to be
distributed to external users such as scientists in other fields, NGOs, consultants, or the interested
public. To that end, the project improves access to existing databases in the museum. It also
cooperates in the construction of an extensive internet-accessible information system (SysTax)
with contributions on several soil and wood inhabiting groups of animals. The geographical focus
was set on the Palearctic and Neotropical regions, and the taxa chosen (Oribatid mites, spiders,
ants, and jewel beetles) are those where years of study have already generated a broad base of
information at the SMNK. The system will include information on collection sites and the
distribution of species as well as on morphological characters and data of their natural history and
ecology. Information will be provided as text, literature references, line drawings, digital light
microscopy and REM-photos, and also as video sequences where convenient. As a service to the
non-specialist user we combine the databased information with easy-to-use digital interactive keys
in DELTA-format [1-4].
Acari: Oribatida
The Oribatida are a group of soil mites. Worldwide about 5,000 species have been described,
with 500 of them occurring in Central Europe. Due to their detritivorous habits, they play an
important functional role in many natural ecosystems. Though of great ecological interest,
identification is currently not possible without the help of a specialist. Descriptions and ecological
information are scattered over a large number of publications, which are often difficult to obtain.
Most of the descriptions available are not suitable for re-identification. Furthermore, the highly
specialized terminology for their external morphology deters students from getting familiar with
this group.
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For this reason, we provide non-verbose interactive keys based on the DELTA-System. The
features of our identification system are:
1. digital images on light microscopy instead of alienating scans made by electron microscopy
2. colour markings of diagnostic characters in figures
3. easy-to-use terminology
In addition, taxonomic, bio-geographical, and ecological information will be provided by
databases connecting literature data to species names. This enables an economic evaluation of
data.
Hymenoptera: Formicidae
In a pilot-project a database will be combined with an interactive DELTA-key to carpenter ant
species of the genus Camponotus with present and/or historical distributions in BadenWürttemberg (BW, South-West Germany). The key will be illustrated by line-drawings, REMand light microscopy photos, and in some cases video sequences of living animals, and thus be of
easy use for specialists as well as non-specialists. The database (SysTax) will include information
on the specimens from the collection of the SMNK (date, sex, locality, habitat, etc.); general
information on the ant species like its distribution in BW; literature references for the species; and
data on individual morphological characters, which can be compared with the species’ standard
descriptors used in the interactive key.
Coleoptera: Buprestidae
The metallic wood-boring or jewel beetles are a group of insects with worldwide distribution,
mainly in the tropics, but at least 80 species can be found in Central Europe. Some of the more
frequent species may cause harm to timber, as they feed on wood during their larval period, like
many species of the family. Others, however, are threatened species protected by law.
Consequently, there is a high demand from foresters and conservationists for identifications of
adult beetles and their larvae.
The latter can be identified with the help of their pronounced traces, i.e. characteristic courses of
tunnels they originate while feeding: some species undermine the cambial layers of branches and
twigs, some bore deeply into the wood. Furthermore, the imago’s hatching hole is of characteristic
outline and enables identification on generic level. Especially if one knows about the feeding
plant, its age and climatic exposition, identification even to species level is possible with the help
of these traces. However, the information about these features is scattered in the literature, and so
specialists with much field experience are needed.
We present a DELTA-based key exemplifying different ways of identification in combining
information on feeding plants, features of larval tunnels and of hatching holes, and characters of
the beetles themselves, thus creating a tool operable by users without expert knowledge.
References:
1. Dallwitz, M. J. 1980: A general system for coding taxonomic descriptions. Taxon, 29, 41–6.
2. Dallwitz, M. J., Paine, T. A., and Zurcher, E. J. 1993: ‘User’s Guide to the DELTA System: a General
System for Processing Taxonomic Descriptions.’ 4th edition.
3. http://biodiversity.uno.edu/delta/
4. ftp://ftp.ento.csiro.au/delta/
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Project ID: 01LC9905 (GLOPP)
1.4.2000-31.3.2003
GLOPP - GLOBAL INFORMATION SYSTEM FOR THE BIODIVERSITY OF
PLANT PATHOGENIC FUNGI
G. Hagedorn1 , G. Deml 1 , D. Triebel2 , M. Piepenbring 3 , and F. Oberwinkler4
1
Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Biologische
Bundesanstalt für Land- und Forstwirtschaft, Königin-Luise-Str. 19, D-14195 Berlin
2
Botanische Staatssammlung München, Menzinger Straße 67, D-80638 München
3
Botanisches Institut, Universität Frankfurt, Senckenberganlage 31-33, D-60054 Frankfurt/M.
4
Lehrstuhl Spezielle Botanik/Mykologie, Botanisches Institut, Universität Tübingen, Auf der
Morgenstelle 1, D-72076 Tübingen
franz.oberwinkler@uni-tuebingen.de
Key words: GLOPP, information systems, fungi, plant pathogens, biodiversity,
DiversityWorkbench, biodiversity informatics
Abstract:
The biodiversity research of several generations of scientists has produced a huge treasure of
biological specimen collections, as well as descriptions and reports of new or known organisms.
Today, this knowledge should be made readily available in the form of digital media. An
integrated information system will greatly improve the efficiency of the work of both the scientists
and the applied users in agriculture and forestry.
Introduction:
The GLOPP project ("Global Information System for the Biodiversity of Plant Pathogenic
Fungi") aims to provide a unified view of data about fungi parasitizing higher plants. The
information system integrates data on host plant specificity, geographical distribution, and a core
set of descriptive characters that allow an interactive online identification. Since our resources are
limited, European pathogens will be treated with priority, to guarantee high data quality and a
certain completeness, but it is planned to continue to extend the scope within a framework of
international, collaborative projects in the future.
The information system that is currently being developed goes beyond a simple compilation. It is
planned as a critical scientific revision of the available data. Uncritical acceptance of
misidentifications and degeneration of data quality will thus be avoided. Original information is
kept strictly separate from assessments and interpretations. It will be possible to document the
(possibly contradictory) assessments of multiple scientists. The system allows the online
interactive identification of plant pathogenic fungi and a retrieval of relevant information, as well
as analyses that increase our understanding of biodiversity. Examples are the diversity of major
taxonomic groups, the specificity of host-pathogen relations, co-evolutionary processes, or
temporal and spatial dynamics in the distribution of pathogens. The project also has a
considerable socio-economic impact due to the large number of economically important
pathogens.
The system is designed to enable farmers or the general public to identify fungal pathogens
without having to study the complete list of characters and without access to the scientific
literature. For example, a query might be: "Show all parasites that belong to imperfect fungi,
which have been found on Rhododendron in Germany, and which have oblong, aseptate spores, 4
x 10 µm large" (fig. 1). The information system would try to find all fungi that fulfil the given
criteria based on past experience (i.e. information published in the literature or in herbaria). The
208
system could provide error tolerant usage, by adding a second result list, which includes all
records from the entire region (i.e. Europe), which have been found on a species in the Ericaceae,
and includes fungi that fulfil the descriptive criteria only approximately. At the end of the project
the information system will be available on the Internet free of charge.
Fig. 1: Prototype for a web interface to use the GLOPP integrated information system
for the identification of plant pathogenic fungi
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Project ID: 01LC9905 (GLOPP, Subproject IT)
1.4.2000 - 31.3.2003
GLOPP-IT - BIOINFORMATICS AND DEVELOPMENT OF THE INFORMATION
SYSTEM
T. Gräfenhan, M. Weiss, M. Gliech, and G. Hagedorn
Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Biologische
Bundesanstalt für Land- und Forstwirtschaft, Königin-Luise-Str. 19, D-14195 Berlin
g.hagedorn@bba.de; http://www.DiversityCampus.net
Key words: GLOPP, information systems, fungi, plant pathogens, identification, bioinformatics,
biodiversity, xml-schema, DiversityWorkbench, DiversityCollection, DiversityReferences,
DeltaAccess, biodiversity informatics
Abstract:
GLOPP-IT is creating an integrated information system on the biodiversity of plant pathogenic
fungi and their hosts by gathering descriptive and collection data, literature references, resource
information and taxonomic names. The project provides a set of application modules to other
projects within GLOPP and develops the public access system on the Internet.
Results:
Many current solutions to manage biodiversity data (e.g. SysTax, Specify, BioLink) are based on
a highly complex, monolithic information model with different tasks tightly connected. These
models are difficult to analyse and implement. The GLOPP IT project has therefore invested
considerable time and effort to design a modular information model, where the various modules
can be implemented separately and are exchangeable with 3rd party modules. Interface definitions
allow to define associations between the central biological objects of the modules. This modular
structure (see fig. 1) provides the basis for a customisable system that can be configured to suit the
demands of scientists and other users. A data exchange format based on XML-schemata is
currently under development. Preliminary versions can be downloaded from the Internet [5-8].
DiversityCollection was designed to capture label data from specimens of plant pathogens in the
herbaria of Munich and Halle/S. (GLOPP-Erysiphales). DiversityReferences manages scientific
literature citations; DiversityTaxonomy records the nomenclature and synonymy of scientific
names and organizes them into (potentially multiple) taxonomic hierarchies.
The information from these three central modules is used in the two modules collecting the data
for the identification process. DiversityDescriptions (derived from DeltaAccess version 1.7)
represents a complete tool to manage descriptive, especially morphological and chemical data.
The SpecialIndexing module records the project specific information on host-pathogeninteractions from literature and specimen data. The identification tool (DiversityIdentify) will use
morphological data as well as geographical and host-parasite-interactions. A web interface will be
implemented during the remaining time of the project to access the identification system from the
Internet.
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Fig. 1: Overview of the modular design of the DiversityWorkbench
Support modules are: DiversityUsers for user administration and documentation, a geographic
names index (DiversityGazetteer, at present including the world-wide Getty TGN and the German
GN250) used for geo-referencing; DiversityResources, to manage hyperlinks, images, and other
media used to support the identification of organisms. DiversityResources indexes resources by
keywords and organism names, addresses the problem of changing URLs, and documents
intellectual property rights.
All modules and information models of the DiversityWorkbench system are open source and
available from http://www.DiversityCampus.net.
References:
1. Hagedorn, G. 2000. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 52, 149.
2. Hagedorn, G., Deml, G., Burhenne, M., Guerrero Cartin, O.M., Gräfenhan, T., and Weiss, M. 2000:
http://www.DiversityCampus.net/Glopp/Presentations/PflSchutzTg2000/PflSchutzTg2000en.html
3. Hagedorn, G. 2001. Bocconea, 13, 261-280.
4. Hagedorn, G., 2001:
http://www.DiversityCampus.net/Glopp/Presentations/GLOPP2001-M/GregorHagedorn-M2001.html
5. Hagedorn, G. 2001: DiversityDescriptions (version 1.8). *
6. Hagedorn, G. and Gräfenhan, T. 2001: DiversityTaxonomy (version 0.6). *
7. Hagedorn, G. 2001: DiversityReferences (version 0.9). *
8. Hagedorn, G. and Weiss, M. 2001: DiversityCollection (beta). *
* References 5-8: All information models, including SQL DDL and xml-schemata documents can be found
under http://www.DiversityCampus.net/Workbench/download.html
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Project ID: 01LC9905 (GLOPP, Subproject LIT)
1.4.2000 - 31.3.2003
GLOPP-LIT - DIGITIZATION OF IMPORTANT EUROPEAN HOST-PATHOGEN
INDICES FOR FUNGI
T. Gräfenhan, M. Burhenne , G. Hagedorn, and G. Deml
Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Biologische
Bundesanstalt für Land- und Forstwirtschaft, Königin-Luise-Str. 19, 14195 Berlin
t.graefenhan@bba.de; http://www.DiversityCampus.net
Key words: GLOPP, fungi, plant pathogens, identification, host-parasite index, Erysiphales,
powdery mildews, Peronosporales, downy mildews, Brandenburger, literature capture,
biodiversity informatics
Abstract:
The GLOPP-LIT project focuses on capturing published or unpublished scientific information on
plant pathogenic fungi. Scientists working in the particular fields of biodiversity will guarantee
the high quality standard of the database. In the framework of the collaborative GLOPP project
the literature project provides a base layer of information for the identification of plant
pathogenic fungi that is supplemented with more detailed information from the projects working
on specific groups. Eventually, scientists will not only be able to search for specific parasite-hostinteractions, also a literature reference and geographic relations are given.
Results:
The most important publication on plant pathogenic fungi for Europe, Brandenburger [1], has
been fully captured in a database of more than 11,200 records. Unfortunately, this publication
does not provide any information on literature references or other resources used in its
compilation. It thus has to be supplemented from index cards and manuscripts provided to the
project by Dr. Brandenburger. The original references on index cards have been scanned, partly
transcribed, and indexed in a networked database (7,600 references). The manuscript (comprising
12 binders) and personal communications are currently ran through to connect the references with
the host-parasite observations. Approximately half of the work has been finished up to now.
In addition two unpublished manuscripts (fig. 1) on the Erysiphales (powdery mildews) and the
Peronosporales (downy mildews) are currently edited in cooperation with Dr. Brandenburger and
converted to structured documents. Based on an XML-like mark-up, they will ultimately be
converted to a relational database and integrated into the GLOPP information system.
212
Fig. 1: Plant parasite record from an unpublished manuscript by Dr. Brandenburger.
The information is identified using named style tags, which are displayed in
different colours, allowing the correction of mark-up mistakes.
Other publications [2-9] have been prepared for conversion into a database. The remaining time of
the project will be necessary to finish the conversion and integration of these sources.
Subsequently specialists of the fungal groups will improve the quality level of the data.
References:
1. Brandenburger, W. 1985: Parasitische Pilze an Gefäßpflanzen in Europa, Stuttgart.
2. Amano, K. (Hirata) 1986: Host range and geographical distribution of the powdery mildew fungi.
3. Braun, U. 1995: The powdery mildews (Erysiphales) of Europe, Jena.
4. Bierlein, J. 1993: Mehltaupilze (Peronosporales, Erysiphales). Regensburger Mykol. Schriften, 2.
5. Brandenburger, W. 1994: Die Verbreitung der in den westlichen Ländern der Bundesrepublik
Deutschland beobachteten Rostpilze (Uredinales). Regensburger Mykol. Schriften, 3.
6. Flachs, K. 1931: Krankheiten und Parasiten der Zierpflanzen, Stuttgart.
7. Gerlach, W. and Nirenberg, H. 1982: The genus Fusarium – a pictorial atlas. Mitteilungen aus der
Biologischen Bundesanstalt für Land- und Forstwirtschaft, 209.
8. Gruber, M. 1993: Uredinales. Regensburger Mykol. Schriften, 2.
9. Pantidou, M. E. 1973: Fungus-host index for Greece, Athens, Kiphissia.
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Project ID: 01LC9905 (GLOPP, Subproject Oomycetes)
1.4.2000-31.3.2003
GLOPP-OOMYCETES
M. Göker1 , A. Riethmüller1 , H. Voglmayr2 , M. Weiß 1 , and F. Oberwinkler1
1
Lehrstuhl Spezielle Botanik/Mykologie, Botanisches Institut, Universität Tübingen, Auf der
Morgenstelle 1, D-72076 Tübingen
2
Institut für Botanik, Universität Wien, Rennweg 14, A-1030 Wien
markus.goeker@uni-tuebingen.de
Key words: GLOPP, data base, biodiversity, Oomycetes, Peronosporales, molecular evolution
Abstract:
A combination of classical morphology and modern molecular methods allows to achieve high
quality of taxonomic data in a biodiversity information system for plant parasitic Oomycetes.
Results:
The Oomycetes are a monophyletic group including obligatory parasitic and saprophytic species.
Members of the Saprolegniales, Sclerosporales, Pythiales and Peronosporales may cause
considerable economic damage on crop plants, e.g. Aphanomyces raphani, causing the radish
black dye, Phytophthora infestans, causing the late blight of potato and Plasmopara viticola,
causing the downy mildew of grapevine.
The GLOPP project does not aim at a simple compilation of data, so critical appraisal of the data
found in taxonomic literature is necessary. As a rule, this entails experimental re-examination of
the published data. Current systematics of plant parasitic Oomycetes, at least above species level,
are based mainly on morphological characters, so extensive light microscopical studies are
required. As optical microscopy is the customary tool in applied phytopathology, quality data
achieved with this technique are of highest practical value.
Unfortunately, the morphological data so far used for Oomycete taxonomy are often ambiguous.
This is especially important where only few morphological characters are available, as it is the
case in species delimitation in most parasitic Oomycete genera. Even the total number of species
in Peronosporales and Sclerosporales stated in the literature can vary very much depending on
the underlying species concept used by different authors [1]. Where species circumscriptions are
based mainly on the taxonomy of the respective hosts [2] and no additional infection experiments
have been carried out, a vicious circle arises with respect to conclusions on cospeciation. The
same is true when practical questions concerning host specificity are considered.
To cope with the mentioned uncertainties, the combination of morphological with modern
molecular methods is required. Application of the latter has been exemplified already in earlier
work from Tübingen [3] with emphasis on the systematics of aquatic Oomycetes. Our recent
publication [4] presenting results from sequence analyses of LSU rDNA focuses on taxonomic
problems in the field of plant parasitic Oomycetes or downy mildews. Below, we will give some
examples from these results and how they influenced our light microscopical analyses and the
arrangement of the Oomycete database.
Important parts of the current classification of the Peronosporomycetidae [5, 6] proved to be
unsatisfactory. With regard to family concepts, we posited that Lagenidium should be placed
within the Pythiaceae, Phytophthora and Peronophythora within the Peronosporaceae.
It could also be shown that the genera Bremiella and Peronophythora should be dismissed and
transferred to Plasmopara and Phytophthora, respectively. Therefore, the character “swollen
conidiophore tips” used so far for the delimitation of Bremiella, is probably of minor taxonomic
214
value. Instead, Bremiella baudysii, a species that parasitizes a member of the Apiaceae, seems to
be most closely related to the Plasmopara species infesting this plant family, a result that gives an
example of the meaning of cospeciation in plant parasitic Oomycetes.
Following the work of Fraymouth [7], we had already erected a morphological typology of the
haustoria in Peronosporales to be used in the database that was clearly supported by the
molecular results. Haustoria seem to be of major importance for the characterization of groups of
genera in Peronosporales. So the Peronospora species possessing haustoria of our type D are
genetically highly diverging from the ones with type C haustoria. Additionally, the former contain
all Peronospora spp. infesting Brassicaceae. Therefore, we focussed microscopical research on
this group. Indeed, we could find some other morphological characters that separate it from the
Peronospora spp. not specialized on Brassicaceae (unpublished data). Likewise, many of the
groups Gäumann [2] called “Formenkreise” were preliminarily supported. However, oospore
characters of Peronospora turned out to be of negligible value for infrageneric taxonomy.
If data quality of an information system about biodiversity is to be guaranteed, three main
interdependent questions arise: (1) Which taxa represent natural groups? (2) Which characters
possess taxonomic significance? (3) How should the character states be defined to achieve a clearcut separation of taxa? Obviously, the content of the database will depend to a high degree on how
these questions are answered. In our opinion, a dual strategy employing morphological and
molecular methods not only facilitates finding the correct answer, but is the only possible way to
proceed. This justifies the often time-consuming search for fresh material of Oomycete-infested
plants and for molecular methods adjusted to this material and the taxonomic problems that have
to be solved. We hope that the examples from our recent molecular and morphological results
given here may support our view.
References:
1. Dick, M. W. 1990: Phylum Oomycota. In: Margulis, L., Corliss, J. O., Melkonian, M., and Chapman, D.
J., Handbook of Protoctista, 33, 661-685, Boston.
2. Gäumann, E. 1923: Beiträge zu einer Monographie der Gattung Peronospora Corda. Beiträge zur
Kryptogamenflora der Schweiz, 5(4), 1-260.
3. Riethmüller, A., Weiß, M., and Oberwinkler, F. 1999: Phylogenetic studies of Saprolegniomycetidae and
related groups based on nuclear large subunit ribosomal DNA sequences. Can. J. Bot., 77, 1790-1800.
4. Riethmüller, A., Voglmayr, H., Göker, M., Weiß, M., and Oberwinkler, F. 2001: Phylogenetic
relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit
ribosomal DNA sequences. Mycologia (submitted).
5. Hawksworth, D. L., Kirk, P. M., Sutton, B. C., and Pegler, D. N. 1995: Ainsworth & Bisby´s Dictionary
of the fungi. 8th ed. Cambridge.
6. Dick, M. W. 2001: The Peronosporomycetes. In: McLaughlin, D. J., McLaughlin, E. G. and Lemke, P.
A. (eds.), The Mycota, VII A, 39-72. Berlin.
7. Fraymouth, J. 1956: Haustoria of the Peronosporales. Transactions of the British Mycological Society,
39: 79-107.
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Project ID: 01LC9905 (GLOPP, Subproject Erysiphales)
1.4.2000 – 31.3.2003
GLOPP-ERYSIPHALES - A GLOBAL INFORMATION SYSTEM FOR THE
BIODIVERSITY OF POWDERY MILDEWS (ERYSIPHALES, ASCOMYCOTA)
C. Kainz1 , M. Weiss1 , U. Braun2 , and D. Triebel1
1
2
Botanische Staatssammlung München, Mycology Dept., Menzinger Str. 67, D-80638 München
Martin-Luther-Universität Halle – Wittenberg, Institut für Geobotanik und Botanischer
Garten, Neuwerk 21, D-06108 Halle (Saale)
triebel@botanik.biologie.uni-muenchen.de ; www.botanik.biologie.uni-muenchen.de/botsamml/
Key words: GLOPP, ColdFusion modules, DiversityCollection, Erysiphales, LabelScan, LIAS,
molecular phylogeny, biodiversity informatics
Abstract:
The labels of 8500 specimens of Erysiphales stored at the state-run herbaria in München and
Halle were digitised using LabelScan. Approximately 20–25 % of the label data have already
been transcribed to the database DiversityCollection. Three ColdFusion modules were developed
for making the data available via the Internet. The molecular studies resulted in 40 complete
sequences of the rDNA ITS region and partial sequences of the rDNA 28S region. An ITS
sequence alignment including 50 Genbank sequences was built up for performing cladistic
analyses. Published descriptive data of all taxa of the Erysiphales have been included in the LIAS
information system and are provided as natural language descriptions. In addition, data can be
queried for interactive identification by the DeltaAccess Perl interface. All data will be evaluated
and presented in a global information system.
Results:
The project Erysiphales uses a number of joined database modules mainly developed within the
GLOPP project (DALI, DiversityCollection, DiversityExsiccatae, DiversityDescriptions). The
biodiversity data were collected from herbarium labels, from original literature and images and –
especially within molecular studies – derived from primary research activities. All types of data
and databases are stored in a modularised database system.
Recording and evaluation of herbarium label data based on the Erysiphales collections in
Halle (HAL) and München (M). In order to solve the problems of recording large amounts of
herbarium data, a concept for a standardized and user-oriented data recording in collections was
developed. In a first stage the material is labelled with barcodes. These labels are scanned together
with the original historical labels showing all information provided by the collector such as
locality data, as well as later revisions. For this purpose the scan-software LabelScan v. 2.0 was
developed to provide an automatic recording of the barcode-labels and a simplified administration
of image files [1]. In another stage the digital label images are imported into DiversityCollection
in order to establish a comfortable and structured way of transcribing the label data into this MS
Access database. The herbarium material is only needed for the scanning of the labels, all
succeeding work can be done within the database, either locally or over the Internet. Due to the
great amount of material (8500 specimens in M and HAL) the transcription process had to be
divided into a number of stages, some of which could be carried out even by untrained persons.
Actually a selective entry of data is performed in three parallel and independent procedures,
namely the entry of collection data (2500 data records already completed), of host data (1200 data
records already completed), and of location data via Getty TGM (600 data records already
completed). The data input into DiversityExsiccatae, a database module with bibliographic
specifications and abbreviations of 220 exsiccata series, is now complete. A pull-up list,
216
containing the basic information of DiversityExsiccatae, was integrated into DiversityCollection
in order to facilitate data entry of the exsiccata series.
Online access to collection data, images and literature of Erysiphales. Several ColdFusion
modules have been developed to give public access to the data via the Internet: “Search
Exsiccatae” is a tool for searching and remote editing the entries in DiversityExsiccatae [2],
“Search Collections” allows a search for host-parasite relationships and collectors in
DiversityCollection [3] and the module “Search Drawings Braun 1987” [4] gives online access to
more than 300 plates with ink drawings originally published by Braun [5]. The literature database
DALI (A Database of Ascomycete Literature) has been extended by the category Erysiphales and
contains 400 pertinent data sets [6].
Data recording for molecular phylogenies (18S, ITS, 28S). The molecular studies on the
phylogeny of Erysiphales resulted in 40 complete sequences of the internal spacer region (ITS) of
the rDNA and partial sequences of the 18S and 28S rDNA. The sequences were aligned with 50
sequences received from GenBank. Alignments of all sequences and several subgroups have been
made. On the basis of these alignments cladistic calculations were performed to get new insights
into the relationships of European taxa of powdery mildews. The analyses of the Uncinula adunca
group are especially promising. Currently the molecular studies are extended to non-ribosomal
genes such as the beta-tubuline coding gene. After successful primer selection the first betatubuline sequences were obtained. The relevancy of this gene for phylogenetic studies on various
systematic levels has still to be tested.
Build-up of a LIAS associated online-system for interactive identification and character
analysis. The descriptive data of Braun [5] were partly transferred to LIAS (A Global Information
System for Lichenized and Non-Lichenized Ascomycetes [7]. For this purpose the LIAS character
list had to be extended to Erysiphales-specific characters, and a list with all species names and
their basionyms including author abbreviations checked for correctness had to be compiled. The
data are now accessible as natural language descriptions [8]. In order to complete the descriptive
data sets with digital illustrations of the organisms (see “Search Drawings Braun 1987”) an
interface for cross linking is under construction. The interface DeltaAccess Perl [9] is used for
establishing an online identification tool for Erysiphales within LIAS [10].
References:
1. Hagedorn, G., Triebel, D., and Weiss, M. 2000: LabelScan: a tool to optimise the establishment of big
collection information systems using selective label data transcription. Presentation and computer
demonstration. In: Anonymous (ed.), TDWG 2000: Digitising Biological collections. Taxonomic Database
Working Group, 16th annual meeting, Abstracts, Mycol. Res., 10, Frankfurt.
2. http://www.mycology.net/exsiccata/ExsiccataFind.cfm
3. http://www.mycology.net/collections/bsm/specimens/CollectionFind.cfm
4. http://www.mycology.net/lias/taxa/drawings/ braun1987/Braun87Find.cfm
5. Braun, U. 1987: A monograph of the Erysiphales (powdery mildews). Bh. Nova Hedwigia, 89, 1-700.
6. http://141.84.65.132/BSM-Mycology/Dali/search.html
7. http://www.mycology.net/lias/index.html
8. http://www.mycology.net/lias/descr/erys33s24/
9. http://www.herbaria.harvard.edu/computerlab/ web_keys/delta_access_perl.html
10. http://www.mycology.net/lias/online/dap.html
217
Project ID: 01LC9905 (GLOPP, Subproject Smut Fungi)
1.4.2000-31.3 2003
GLOPP – SMUT FUNGI
M. Piepenbring 2 , C.-L. Hou1 , G. Hagedorn3 , G. Deml3 , and F. Oberwinkler1
1
Lehrstuhl Spezielle Botanik/Mykologie, Botanisches Institut, Universität Tübingen, Auf der
Morgenstelle 1, D-72076 Tübingen
2
Botanisches Institut, Universität Frankfurt, Senckenberganlage 31-33, D-60054 Frankfurt/M
3
Institut für Pflanzenvirologie, Mikrobiologie und Biologische Sicherheit, Biologische
Bundesanstalt für Land- und Forstwirtschaft, Königin-Luise-Str. 19, D-14195 Berlin
piepenbring@em.uni-frankfurt.de
Key words: GLOPP, smut fungi, Tilletiales, Ustilaginales, China, DiversityDescriptions,
biodiversity informatics
Abstract:
Data on the morphology of smut fungi are integrated into the GLOPP information system using
the module DiversityDescriptions. Up to now, 460 different species of smut fungi have been
documented. Field research conducted in the Neotropics and in Yunnan, China, provided
collections of fresh material of smut fungi, which was used to study the morphology and alphataxonomy. These primary data significantly improved the quality of species information in the
database.
Results:
The software DiversityDescriptions (see GLOPP-IT) presently offers 169 characters with
numerous character states for the description of species of smut fungi. These characters and
character states (descriptors) are based on those observed in detail by M. Piepenbring on
neotropical smut fungi for which a monograph for Flora Neotropica is prepared. The monograph
includes 227 species in 34 genera, or ca. 17.5 % of the about 1,300 species of smut fungi known
world-wide. With more species of smut fungi added to the database, further characters and/or
character states have to be added. The database allows this at any time, without affecting existing
data sets.
As many as possible species will be studied by us personally before including them into the
database. Further species are included based on descriptions in literature we consider reliable.
European smut fungi, for example, are relatively well known by a monograph written by Vánky
[1], descriptions of North American smut fungi can be found in the book of Fischer [2]. The
character states of species descriptions in literature are transferred into the characters and
character states available in GLOPP-DiversityDescriptions. In some cases like size and colour
characters, this is a relatively straightforward task, but e.g. for soral morphology and for spore
ornamentation, different authors use different terminology. Illustrations like detailed drawings and
SEM photographs of teliospores can help, but a broad knowledge of the diversity of
morphological features of smut fungi is indispensable to avoid transcription errors.
By now 460 more or less complete and revised species descriptor sets are present in the
DiversityDescriptions database. We intend to include descriptions of most of the known species
of smut fungi until the end of the project, however, present knowledge on many species of smut
fungi is limited in several ways:
• Details of soral morphology are only evident in good material including young states of
development.
218
•
Some species are known by only one or few poor collections which do not always allow a
detailed study of morphology nor the identification of the host plant.
• The identification of host plants documented in literature is not always reliable.
• For many species we do not know the morphology of the basidia, which can only be observed
with recently collected teliospores placed on water agar.
• There are many taxonomic problems including unresolved synonymy, problematic generic
placements, and unclear delimitation of species.
In addition to these problems, we are far from knowing the really existing diversity of smut fungi,
especially in large countries where few mycologists looked for this group.
In order to improve the quality of data for poorly known species, field work is a constant part of
our investigations. In Bolivia, with only five weeks of field work and study of herbarium
specimens, four new species were found [3].
For China the diversity of Ustilaginales is documented by Guo Lin [4]. She described 140 species
of this order for China, which has regions with very high diversity of higher plants. This can only
be a small proportion of the existing diversity of smut fungi, because for Europe, with less
diversity of higher plants, we know ca. 400 species of Ustilaginales and Tilletiales, with the
Ustilaginales being the larger group [1]. Fieldwork and study of herbarium specimens in Yunnan
during the summer of 2001 led to 50 collections representing 25 species. These can now be
examined in detail and the respective descriptor sets can be completed in the database, including
e.g. the morphology of basidia. Among the species are new ones, new records for the region, and
some of them occur on species hitherto unknown as host. More field work of this kind, however,
is necessary to come to a more realistic assessment of the diversity present in China.
Recently collected specimens of smut fungi can be used for molecular analyses. Phylogenetic
hypotheses based on LSU rDNA provide important arguments for systematic and taxonomic
conclusions. They directly improve the quality of the data in the database by indicating generic
placements of the species. The delimitation of characters and character states is also affected
because molecular phylogenies allow to distinguish similar character states based on homologies
from those based on analogies. This has recently been demonstrated for species of Cintractia by
Piepenbring et al. [5].
While field research on smut fungi is going on, we complete the database hoping to encourage
more mycologists and taxonomists of higher plants to look for this group of plant parasitic fungi,
which is not only of economic importance but also a fascinating example on how parasitic fungi
coexist with their host plants in a natural balance.
References:
1. Vánky, K. 1994: European smut fungi. Stuttgart, New York.
2. Fischer, G. W. 1953: Manual of the North American fungi. New York.
3. Piepenbring, M. 2001: New species from the neotropics. Mycol. Res., in press.
4. Lin, G. 2000: Ustilaginaceae. Flora Fungorum Sinicorum, 12. Beijing.
5. Piepenbring, M., Begerow, D., and Oberwinkler, F. 1999: Molecular sequence data assess the value of
morphological characteristics for a phylogenetic classification of the species of Cintractia. Mycologia, 91,
485-498.
219
Project ID: 01LC9905 (GLOPP, Subproject Uredinales)
1.4.2000-31.3.2003
GLOPP-UREDINALES - REGISTRATION OF TROPICAL AND SUBTROPICAL
RUST FUNGI
Reinhard Berndt, Anja Ritschel, and Franz Oberwinkler
Lehrstuhl Spezielle Botanik/Mykologie, Botanisches Institut, Universität Tübingen, Auf der
Morgenstelle 1, D-72076 Tübingen
reinhard.berndt@uni-tuebingen.de
Key words: biodiversity-database, Costa Rica, Prospodium, Uredinales
Abstract:
Rust fungi are economically important but still insufficiently studied in tropical regions. In our
approach we ascertain data on taxonomy, distribution and ecology of tropical rust fungi and
complement them by basic systematic and field research. This combination yields high quality
data that are essential for the applied research and utilization of taxonomic knowledge e.g. in
agriculture as well as to solve elementary questions of rust fungal biology.
Introduction and research plan:
With about 7.000 species, rust fungi are the biggest and economically most important group of
obligate plant parasites. Beside their economic importance, rust fungi have a uniquely
complicated life cycle and offer excellent opportunities for the study of plant-parasite coevolution. The knowledge about biodiversity, distribution and host relationship of rust fungi from
the tropics and subtropics is still restricted and difficult to access as summarising floras and
monographs are widely lacking.
Our work aims to contribute to the investigation of tropical and subtropical rust mycota
(1) by providing data on the taxonomy, distribution and host selection of the parasites
(2) and by ascertaining own field and systematic data.
Only the permanent control of literature data by accompanying critical elementary studies can
guarantee a reliable data quality.
In our approach we concentrate on three subjects:
•
a study of the rust fungus mycota of Costa Rica
•
a monographic study of the neotropical rust genus Prospodium
•
the building of a database/checklist of tropical and subtropical rust fungi containing data
on nomenclature, distribution and host spectra.
Results:
Rust fungus mycota of Costa Rica. During our exploratory trips to Costa Rica we secured
approx. 300 rust specimens, of which 250 are determined and registered in the database. Among
the collected specimens we found seven species that were new for science. Several species
represented new reports for Costa Rica or were found on hosts that had not been recognised
before. In the next step the important Döbbeler-collection of Costa Rican rusts will be evaluated.
So far, 220 species of rust fungi have been ascertained for Costa Rica from literature.
220
Prospodium, a neotropical genus of rust fungi. We selected the genus Prospodium for a case
study for several reasons: with approx. 70 species the genus is exclusively neotropical and shows
a strict host restriction on Bignoniaceae and Verbenaceae. Its tropical distribution, species number
and morphological diversity makes Prospodium an ideal example to combine a monographic
study with processing the data in a character and biodiversity database. Up to now, a species- and
synonym list have been built up. Two new species, Prospodium manabii and P. kisimovae, were
collected in Ecuador and Costa Rica.
Database/checklist of tropical and subtropical rust fungi. We are working on a database that
contains nomenclatural information as well as data on distribution and host spectra of rusts. At the
present stage, mainly literature data on tropical and subtropical species are included and is being
critically evaluated. The database presently contains information on approx. 13.000 parasite-host
combinations that are represented by ca 21.000 geographical reports.
221
Project ID: 01LC9906 (INFOBOT)
1.4.2000 – 31.3.2003
INFOBOT - DEVELOPMENT AND TESTING OF INFORMATION SYSTEMS
COVERING DIFFERENT ASPECTS OF BOTANICAL COLLECTIONS
W. G. Berendsohn (project coordinator) and A. Vasiliou
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
w.berendsohn@bgbm.org; http://www.bgbm.org/BioDivInf/
Keywords: information systems, botany, vascular plants, Compositae, living collection, natural
history collection, botanical garden, natural substances, biodiversity informatics
Abstract:
INFOBOT demonstrates the application of information technology on different aspects of
botanical collections: the management of living collections, the linking of factual information
(chemical compounds) important in applied research, and access to type specimen information as
one of the keys to basic taxonomic research. The resulting information systems are to be
published on the WWW and will be integrated into information networks.
Introduction. INFOBOT is a collaborative project creating information systems about different
aspects of plant collections. Two of the three partial projects focus on the world’s largest vascular
plant family, the Compositae. Although they lack the urgently needed central taxonomic
component (a global species database of the family), the two projects demonstrate different
approaches towards the mobilization of taxon information in electronic databases. The third
project incorporates the aspect of living collections. The potential of botanic gardens as a source
of information and materials for education and research will not be fully realized until their
information is available on-line. At the same time, information technology greatly assists in the
internal management and quality control of the information held and produced by such
collections.
INFOCOMP/TYPES uses digital photography (see fig. 1 and following article) in combination
with textual input to provide access to nomenclatural type specimens (vouchers on which the
species’ description was based and which fix the application of the scientific name). The types of
the Compositae present in the most important German herbaria are being recorded and will be
made accessible to scientists on the WWW.
INFOCOMP/CHEM , the “Bohlmann Files”, is a database of all natural substances in the
Compositae. A former card index which was transferred to an ISIS/PC database is now converted
to a relational database format and will be accessible on the WWW. In this database chemical and
botanical expertise is united, providing combined information which is of great potential for a
wide range of users, e.g. in pharmaceutic industry and biotechnology. The project pinpoints the
diversity of natural substances and will contribute to the taxonomic research attempting to
elucidate the relationships and evolution within this important group of plants.
INFOBOT-BoGART, the Garden Accessions Database of the Botanic Garden Berlin-Dahlem, is
a model project for a complete accession and administration of the living collection of a botanic
garden. This system provides effective access to the actual living stock of vascular plants of one
of the largest living collections world-wide. It is a program for the data management of a botanic
garden and acts as a basis for further scientific research. With the linking of the database to the
WWW, the information will be accessible to a wide range of users and can also be connected to
other databases and information systems dealing with biodiversity information.
222
Fig. 1: A botanical type specimen (INFOCOMP/TYPES). The rules of botanical nomenclature
prescribe that any taxonomic group named like this specimen (or carrying a name based on this
name) will have to include this specimen. Type specimens thus form the essential base of the
process of naming taxonomic concepts.
223
Project ID: 01LC9906 (INFOBOT, Subproject INFOCOMP/TYPES)
1.4.2000 – 31.3.2003
COMPOSITAE TYPES IN GERMAN HERBARIA
P. Bodensteiner, A. M. R. Davies, A. Pillukat, B. Grau, F. Schuhwerk, and J. Grau
Botanische Staatssammlung München, Menzinger Straße 67, D-80838 München
Contact: grsystau@botanik.biologie.uni-muenchen.de
Key words: types, Compositae, specimen images, German herbaria, biodiversity informatics
Abstract. Of the estimated 2,700 Compositae types held in Munich, 1,533 have been
photographed, resulting in approximately 7,600 high-resolution images. All the bibliographic
information has been checked and linked to the images. The completed data is gradually being
made available on the Internet, using the software "Filemaker Pro".
Introduction. The Compositae are the largest family of seed plants with an estimated 25,000
species [1]. They are found in all places colonised by higher plants on all continents and often
form significant parts of the most diverse ecosystems [2]. They are not only scientifically
interesting but also have economically important representatives. Nomenclatural types are the
reference sheets of pressed plants laid down in herbaria. These are directly and permanently
connected to their plant names and allow species to be unambiguously identified. They thus
underpin even the most modern research techniques (see [3]). The more traditional and larger a
herbarium, the richer it will be in types. For historical reasons, many types originate from
developing countries and have attained value through European botanical research. With this
project it is now possible to contribute to data repatriation by making this information globally
available to scientists.
The project was established primarily as a pilot scheme for the digital recording of essential
components of German herbaria. The project is supported by three graduate scientists and, from
time to time, a Database/Network administrator and the current excellent co-ordination of the staff
has greatly increased the speed of progress in recent months. With the experience gained, digital
imaging in targeted spheres can now be rapidly and successfully completed.
Fig. 7: Original label
Fig. 8: Capitulum
Results. Initial teething problems have now been resolved and image format and quality
standardised. On average, five images per type are taken: the original label(s) (fig. 1) [necessary
when determining provenance and authorship of older sheets, as well as for subsequent data
capture from the images], the entire sheet (see previous page), relevant habit detail, and macro
images of taxonomically important features - usually the capitulum(a) (fig. 2, 3). Special emphasis
is placed upon the correct and accurate citation of the protologue.
224
Figure 9: Detail of capitulum necessary for species identification
Types from all seventeen of the Compositae tribes have been recorded (fig. 4). In total 350 genera
and 1,533 types have been photographed, forming a database of over 7,600 images. Awaiting a
fully functional interface for image storage and retrieval in the database system SYSTAX, the
software "iView MediaPro" has been used to organize image and text data, retaining flexibility for
other software options. The database software used to capture data, "Filemaker Pro", provides an
integrated web interface allowing direct access to the database via the World Wide Web. It is
anticipated that the work in Munich, including the preparation of the information for Internet
access, will be completed during the next year.
Vernonieae (212)
Heliantheae (194) Gnaphalieae (196)
Senecioneae (166)
Astereae (153)
Remaining Taxa
(c. 1100)
Cardueae (141)
Lactuceae (94)
Calenduleae (5)
Barnadesieae (6)
Anthemideae (93)
Mutisieae (73)
Liabeae (8)
Eupatorieae (63)
Arctoteae (14)
Inuleae (50)
Plucheeae (19)
Helenieae (46)
Fig. 4: Number of Compositae Types in Munich (M) photographed
(as of Sept. 2001) arranged by Tribe (after [4])
References:
1. Heywood, V. H. 1993: Flowering Plants of the World. London.
2. Heywood, V. H., Harborne, J. B., and Turner, B. L. 1977: An overture to the Compositae. In:
Heywood, V. H., Harborne, J. B., and Turner, B. L. (eds), The Biology and Chemistry of the
Compositae, 1, 1–20, London.
3. Hind, D. N. J., Jeffrey, C., and Pope, G. V. (eds) 1995: Advances in Compositae Systematics. Kew.
4. Bremer, K. 1994: Asteraceae, Cladistics and Classification. Portland, Oregon.
225
Project ID: 01LC9906 (INFOBOT, Subproject INFOCOMP/BOHLMANN)
1.8.2000 – 31.05.2002
THE BOHLMANN-FILES – A DATABASE OF NATURAL SUBSTANCES IN THE
COMPOSITAE
A. Hahn, J. Jakupovic, B. Smalla, F. Tsichrintzis, C. Zdero, and W. G. Berendsohn
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
a.hahn@bgbm.org; http://www.bgbm.fu-berlin.de/BioDivInf/projects/bohlmannfiles/
Key words: Compositae, Asteraceae, natural substances, chemotaxonomy, chemical database,
taxonomic database, secondary metabolites, biodiversity informatics
Abstract:
The Compositae (Asteraceae) contain numerous chemical compounds, many of them with a high
potential in, e.g., pharmaceutical utilisation. A relational database was designed holding almost
20,000 chemical structures from about 5000 taxa. The revision of plant names and chemical data
is almost concluded, reviewing of synonymy is under way, and publication on the World Wide
Web will follow in the near future.
Introduction. Members of the Compositae are renown for their contents of a variety of
physiologically active compounds [1], for example, they are used as remedies such as chamomile,
insecticides such as pyrethrinoids, or pharmaceuticals such as the antimalarial agent Artemisinin
from Artemisia annua. The late Ferdinand Bohlmann, professor at the Technical University of
Berlin, dedicated much of his life to the investigation of these substances [2]. Together with his
technical assistant C. Zdero he started in the beginning of the 1960ies to compile a card index on
natural substances and taxa of the Compositae, encompassing both data from original research
and from literature. About 95% of the data are published. In 1994, Zdero and one of Bohlmann’s
pupils, J. Jakupovic, started to transfer the data to an ISISBase chemical database, constantly
adding data from more recent publications.
The project. At the outset of the present project, the database held data on about 5000 taxa, with a
total of 19,351 chemical structures. In addition, (trivial) name of the compound, molecular weight
and formula, literature references, and various revision notes were on record. The ISISBase
program allows searching on structures and partial structures as well as on the contents of the text
fields. However, ISISBase is not designed for the publication of small datasets on the World Wide
Web, and it cannot easily be linked to other data sources. The Bohlmann Files project was thus
proposed to meet three main objectives: (i) Convert the database into a relational system and
transfer all data, (ii) revise the chemical and nomenclatural data and fill in remaining gaps from
literature, and (iii) make the database accessible via the WWW, including the search features of a
chemical database (see fig. 1).
Results. The data is considered reasonably complete for secondary metabolites found up to now
in the Compositae. Providing free access to this wealth of information will not only serve
applied sciences (e.g. to identify potential sources for substances), but also enhance basic
research, e.g. by providing additional clues to the taxonomy of groups considered to be
paraphyletic (e.g. the tribe Lactuceae) or not yet assigned to a tribe (e.g. the genera Corymbium
and Centipeda ) and to elucidate end-products of gene expression and biosynthetic pathways.
The project database has been set up under MS Access 2000, adapting the taxonomic module of
the BoGART-database system (see next project) for the treatment of identification and
nomenclature. Data from the flat-file format ISIS database were parsed to separate and atomise
plant names, synonyms, and literature data. Chemical structures are stored using the add-in
226
“Accord” software. Its structural search capabilities allow searches via the WWW and thus form
the core of the web publishing component which will be released before long.
Query: A (sub-)structure can be
drawn or input in textual form in the
WWW query form can be made
Result: The result consists of
secondary metabolites containing the
structure and (among other data) the
taxa they were isolated from
Artemisia douglasiana Besser
Dendranthema maximowiczii (Komar.) Tzvelev
Athanasia dregeana Harv.
Dendranthema maximowiczii (Komar.) Tzvelev
Fig. 1: Searching for a substructure in the database
Plant names have undergone a revision process including spell checking, standardisation
(including author citations), and a check against major web-based databases on plant names [3, 4].
They now reflect the recent classification of the Compositae [5]. The data conform to the TDWG
standard on names [6] complete with source citations for taxonomic data. Literature citations are
kept in a separate reference list. Further taxonomic and bibliographic information and data on the
bioactivity or use of compounds or the plant itself can be linked at a later date. Taxa from about
755 genera represent all tribes within the 3 subfamilies.
References:
1. Heywood, V. H., Harborne, J. B., and Turner, B. L. (eds) 1977: The Biology and Chemistry of the
Compositae. 1, 1–20, London.
2. Winterfeldt, E. 1994: Ferdinand Bohlmann und sein wissenschaftliches Werk. Liebigs Ann. Chem.,
1994, 1-34.
3. Plant Names Project 1999: International Plant Names Index. Published on the Internet;
http://www.ipni.org
4. Missouri Botanical Garden. VAST (VAScular Tropicos) Nomenclatural Database and Associated
Authority
Files,
Published
on
the
Internet
as
W3 TROPICOS
release
1.5;
http://mobot.mobot.org/W3T/Search/vast.html
5. Bremer, K. 1994: Astereaceae – Cladistics and Classification. Portland.
6. Bisby, F. 1994: Plant Names in Botanical Databases, TDWG Standard No.3, Version 1.00. 1994;
http://www.tdwg.org/plants.html
227
Project ID: 01LC9906 (INFOBOT, Subproject BoGART)
1.4.2000 – 30.06.2002
BOGART - THE INFORMATION SYSTEM OF THE BERLIN BOTANIC GARDEN
A. Hahn, A. Kirchhoff, U. Matthäs, A. Surma, and W. G. Berendsohn
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
w.berendsohn@bgbm.org; http://www.bgbm.fu-berlin.de/BioDivInf/Projects/Bogart-d.htm
Key words: biological collection, botanical garden, living collection, botany, taxonomy,
information system, biodiversity informatics, accessions
Abstract: The “BoGART” database system of the BGBM demonstrates system development in a
large living collection. The original system has been converted to a client server environment.
Existing data (electronic or in writing) were transferred to the new system and the user interface
adapted to specific tasks in the management of the garden’s collection. A prototype XML wrapper
provides access for networks and local or remote query interfaces.
Introduction. With more than 20,000 taxa and 35,000 accessions on 43 ha, the Botanic Garden
Berlin-Dahlem is one of the world’s largest gardens. It is a scientific research collection, an
educational exposition, and at the same time a recreational site for the public. Collections of this
size not only demand constant physical care but also require an elaborate system to administer the
information flow for scientific as well as practical work (see fig. 1). Detailed tracking of
information on provenance and handling of accessions is an obligation with regard to the CBD’s
(Convention on Biological Diversity) demand for benefit sharing with countries of origin.
Adequate documentation is also a pre-requisite for the botanical garden’s role in species
conservation [1].
INFOBOT-BoGART will complete the last phase in the process of transferring this system from
the labour intensive index card system of the past to a networked electronic information system. It
upgrades the internal prototype system to a scalable and publicly available multi-user application,
based on widely used technology (SQL-Server, MS Access, WWW). It builds on several prior
projects, starting in the 1980ies, which provided a sound foundation of evaluation, modelling, and
implementation (see [2] for further historical details).
Results regarding data import, input, control and standardization. The import of static
electronic data files from existing isolated applications within the garden has been finished. Data
input of taxonomic information was completed and the data content has been checked against the
card files. The system now contains information on more than 38,000 taxon names. Likewise,
almost 37,000 accession datasets from imports have been checked, supplemented and partially
reworked, achieving a high level of detail e.g. on the original provenance of the plants. Data
standardization was a major focal point, concentrating on the areas of literature data ( ca. 1450
citations), geography, and names and authors of plant taxa.
Technical results. The complex data model, as well as the increased data content had severely
limited the performance of the system. True multi-user functionality was achieved with the
conversion of the Access97 database into an Access2000 project. The backend resides in an MS
SQL-Server2000 client-server database, and the Access-frontend’s client connection has been
converted from ODBC to OLE DB. The new system provides efficient parallel access from
workstations distributed throughout the Botanic Garden area. Work in progress includes further
performance optimisation, transferring functionality from Visual Basic code in the front end to
server-sided stored procedures and triggers.
228
Networking. An XML-DTD was prepared which covers all data items provided by the
BoGART system as well as concatenated or converted items demanded by exchange standards
such as the ITF format [3]. Based on the DTD, a prototype XML wrapper has been
programmed, which will be used to connect the database to national and international networks.
Integration in the work process. The ongoing final period of the project is focussed on the
implementation of specific functionalities, supporting e.g. inventory processes, the scientific
scrutiny of taxon names and linked data (synonyms, common names, species distribution,
poisonous, medicinal, and protected plants, literature), the identification process, the collection
and documentation of herbarium specimens, etc. The functions are developed in close cooperation
with the respective user groups.
Cultivation
Documentation
Services
Processing
provenance information
required by the CBD
2000 new accessions and ~900
scientific identifications p.a.
inventory data from garden
departments
voucher collections for garden
herbarium
Monitoring
endangered plants and
research collections
BoGART
Index Seminum (international
seed exchange)
Supply of plant material for
science and education
Visitor information
Local network access
On-line access through
BGBM website and Botanical
Garden Information System
(VBG/Systax) and
other networks
Science
Identification
Incorporation of taxonomic
research results
Species conservation
Research collections
Fig. 1: Information domain of the BoGART program
References:
1. Maunder, M., Higgens, S., and Culham, A. 2001: The effectiveness of botanic garden collections in
supporting plant conservation. Biodiversity & Conservation, 10, 383-401.
2. Elankovan, S. and Meyer, H. 1996: Analyse, Modellierung und Design einer taxonomischen und
sammlungsbezogenen relationalen Datenbasis zur Verwaltung von Akzessionsdaten im Bereich der
Botanik. Diplomarbeit TU Berlin, FB Wissensbasierte Systeme.
(http://www.bgbm.org/projects/examensarbeiten/elankovanMeyer/)
3. Jackson, D. (compiler) 1997: International Transfer Format for Botanic Garden Plant Records (version
2.00 draft 3.2.). Richmond. [Proposed TDWG standard] [Electronic version:
http://www.rbgkew.org.uk/BGCI/news.htm ]
229
Project ID: 01LC0026 (AlgaTerra)
1.10.2001-30.09.2004
ALGATERRA – AN INFORMATION SYSTEM FOR TERRESTRIAL ALGAL
BIODIVERSITY: A SYNTHESIS OF TAXONOMIC, MOLECULAR AND
ECOLOGICAL INFORMATION
R. Jahn (project coordinator)
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
r.jahn@bgbm.org; http://www.algaterra.net/
Key words: information system, Internet accessibility, biodiversity, algae, diatoms, green algae,
cyanobacteria, types, taxonomy, nomenclature, Ehrenberg, Hustedt, collection data, literature
data, identification, morphology, culture strains, rDNA, sequence analyses, phylogeny, ecological
data, biodiversity informatics
Abstract:
Aim of this recently started project is to build a comprehensive information system on terrestrial
micro-algae, integrating taxon, type, name and collection data as well as ecological and
molecular information. The main target groups will be scientists pursuing basic research, such as
taxonomy and biodiversity studies, as well as applied researchers, such as ecologists, limnologists
[1], and public agencies, engaged in biomonitoring for public health and environmental
conservation. The system will be made available on the Internet (World Wide Web).
Background:
Microscopic algae have been recognized as useful tools in global change research, e.g. for
monitoring water quality indicating trophic status, acidification, and salinization, for
reconstruction of paleoclimates through sediments, and for the assessment of biodiversity of
diverse ecosystems. The taxonomy and in particular the nomenclature used to record these
observations is often ambiguous, leading to incoherent data sets published by different researchers
that need to be synthesized for global studies. The available ecological information for these taxa
is thus doubtful and modern molecular information cannot be assessed correctly. The problems
are frequently due to poorly documented nomenclatural types as well as to divergent secondary
interpretations of the taxon concept behind the name.
The network:
The BGBM Berlin-Dahlem (see AlgaTerra – AT1) will be responsible for the development of the
comprehensive information system, and for data evaluation and typification of selected taxa
housed at the Ehrenberg Collection. The other participants of the AlgaTerra network are the
Museum für Naturkunde Berlin (Ehrenberg Collection, see AT2), the Alfred Wegener Institute,
Foundation for Polar and Marine Research (type data of the Hustedt Collection, molecular,
morphological and ecological data of diatoms, see AT3), the Culture Collection of Algae at the
University of Göttingen (morphological and molecular data of green and bluegreen algae, see
AT4), and the University of Leipzig (ecological and applied data of aero-terrestrial algae, see
AT5). This cooperation (see fig. 1) will synthesize taxonomic, molecular and ecological
information from all subprojects for worldwide accessibility.
230
Fig. 1: The AlgaTerra network: Subprojects, topics and information flow
References:
1. Kusber, W.-H. and Jahn, R. 2002: Standards für die Artidentifikation von Algen in der Limnologischen
Forschung. Tagungsband der Jahrestagung 2001 der Deutschen Gesellschaft für Limnologie (DGL) in
Kiel, in press.
231
Project ID: 01LC0026 (AlgaTerra Subproject 1)
1.10.2001-30.09.2004
THE ALGATERRA INFORMATION SYSTEM INCLUDING THE IDEAL-MODULE
W.-H. Kusber, K. Teske , and R. Jahn
Department of Biodiversity Informatics and Laboratories, Botanic Garden and Botanical Museum
Berlin-Dahlem, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin
w.h.kusber@bgbm.org, k.teske@bgbm.org, r.jahn@bgbm.org; http://www.bgbm.org/jahn/default.htm
Key words: types, taxonomy, nomenclature, identification, morphology, algae, diatoms,
biodiversity, Ehrenberg, database, internet accessibility, biodiversity informatics
Abstract:
A comprehensive Internet Information System for terrestrial algal biodiversity as a synthesis of
taxonomic, molecular and ecological information (AlgaTerra) will be developed and
implemented. The IDEAL-Module (Internet Database of Ehrenberg’s Algae Lectotypes) will
supply original data on algal types as a calibration tool for the identification of micro-algae. The
Information System will be managed and supported over the long-term by the Botanic Garden
and Botanical Museum Berlin-Dahlem as part of its scientific information services.
Results and research plan:
The BGBM Berlin-Dahlem will be responsible for the information system and for data
evaluation.
The IDEAL-Module will give access to original data of selected taxa described by C.G.
Ehrenberg (1795-1876). The relational database will include the validated name of the type, the
original diagnosis, type locality information, a digital photograph of the selected type, and, where
possible, a SEM-analysis of the original material (for Ehrenberg’s scientific life and taxonomic
concept, see [1, 2]). The database will also supply possible synonyms, notes of experts plus links
to updated nomenclature, modern taxonomic interpretations as well as biogeographic, ecologic
and molecular data.
Typification of Ehrenberg-taxa, housed in the Ehrenberg-Collection ([3], see AT2) was
established and published for a small number of genera [4, 5] and species [6, 7, 8], but no data is
accessible via Internet.
The main steps of data preparation for the IDEAL-Module are:
• digitalization of the algal name and description
• translation of the original description
• validity check according to the rules of nomenclature
• digitalization of the published picture
• digital photograph of the chosen (lecto)-type
Quality evaluation of data will include:
• taxon concept of the data source (fig. 1)
• type data versus interpreted data (observation data, applied data)
The development of the information system [9] will include:
• elaboration of an appropriate data model and database design
• programming and implementation of the database
• development of a user-oriented web-interface
232
•
establishment of the connection between database and Internet
Fig. 1: Example of an unpublished drawing by C.G. Ehrenberg. This drawing sheet of 1834 shows his taxonomic
concept, representing at least five taxa in our current understanding. It includes manuscript names, data on
collecting dates as well as size measurements which are to be compared to his published data (fig. provided by
AT2).
References:
1. Jahn, R. 1998: C. G. Ehrenberg: The man and his botanical science. Special Publication of the Linnean
Society, 1, 15-29.
2. Jahn, R. 1995: C. G. Ehrenberg’s concept of the diatoms. Arch. Protistenk., 146, 109-116.
3. Lazarus, D. B. and Jahn, R. 1998: Using the Ehrenberg Collection. Diatom Res., 13, 273-291.
4. Lange-Bertalot, H. and Jahn, R. 2001: On the identity of Navicula (Frustulia) rhomboides and Frustulia
saxonica (Bacillariophyceae). Syst. Geogr. Pl. “2000”, 70, 255-261.
5. Crawford, R. M., Likhoshway, Y. V., and Jahn, R.: The morphology and identity of Aulacoseira italica
and the type of the name of the genus Aulacoseira. Diatom Res., submitted.
6. Jahn, R. 1995: Ehrenberg’s marked species on five mica. Iconogr. Diatomol., 1, 101-107.
7. McLachlan, J. L., Boalch, G. T., and Jahn, R. 1997: Reinstatement of the genus Exuviaella
(Dinophyceae, Prorocentrophycidae) and an assessment of Prorocentrum lima. Phycologia, 36, 38-46.
8. Crawford, R. M. and Likhoshway, Y. V. 1999: The frustule structure of original material of Aulacoseira
distans (Ehrenberg) Simonsen. Diatom Res., 14, 239-250.
9. Jahn, R., Kusber, W.-H., and Teske, K. 2001: The project AlgaTerra: Outline of an information system
for terrestrial algal biodiversity. Poster and Abstract TDWG, Sydney.
233
Project ID: 01LC0026 (AlgaTerra Subproject 2)
1.10.2001-30.09.2004
ACCESSIBILITY OF THE EHRENBERG COLLECTION
D. Lazarus and A. Jobst
Instititut für Paläontologie, Museum für Naturkunde, Humboldt-Universität zu Berlin,
Invalidenstr. 43, D-10115 Berlin
david.lazarus@rz.hu-berlin.de; http://www.museum.hu-berlin.de/pal/microp/david.lazarus.htm
Key words: Ehrenberg, algae, diatoms, collection data, types, original materials, internet
accessibility, biodiversity informatics
Abstract:
The Ehrenberg Collection holds the scientific legacy of Christian Gottfried Ehrenberg (17951876), generally considered to be one of the founders of both microbiology and micropaleontology. Ehrenberg was one of the most taxonomically prolific of all scientists, and the type
specimens for several thousand algal species and several hundred algal genera are present in the
collection. These include many of the most common species and genera names now in use, and
the collection includes the types and original material for many of the species and genera
(diatoms and also other algae) that will be examined in the AlgaTerra project.
Introduction:
The collection was deposited with the Museum für Naturkunde in Berlin upon Ehrenberg’s death,
and has been preserved through the years in good physical condition. Experience in working with
the collection has shown that the majority of all type material is indeed present in the collection
(fig. 1). However, locating these type materials in the Ehrenberg Collection is currently very
difficult, as the collection has not been adequately curated since it was deposited with the
Museum more than 100 years ago, and the original collection structure is not well suited to
modern research needs [1, 2, 3].
Fig. 1: Number of types for genera in Ehrenberg Collection
0
20
40
60
diatoms
green algae
phytoflagellates
radiolarians
ciliates
other protists
234
80
100
120
The project. The goal of this subproject is to carry out the minimum amount of basic inventory,
labelling, archiving and database work (fig. 2) needed to allow other subprojects to use the
Ehrenberg Collection efficiently in their own work. The work will result in a database of the
Ehrenberg Collection; properly labelled, sorted collection objects; working image copies of fragile
original documents (see AT1, fig. 1); and, for those taxa selected for study by the project as a
whole, data extracts from the Ehrenberg Collection database that will be imported into the main
project database (see AlgaTerra – AT1). The Ehrenberg Collection database will be maintained at
the Museum für Naturkunde and will be accessible online via standard Internet protocols.
Fig. 2: Mica strip holding five mica preparations with original material and information; to be catalogued in this
subproject
References:
1. Lazarus, D. B. 1998: The Ehrenberg Collection and its curation. In: Williams, D.M. and Huxley, R.
(eds), Christian Gottfried Ehrenburg [sic] (1795-1876): The man and his legacy. The Linnean Society,
Special Publication, 1, 31-48.
2. Lazarus, D. B. and Jahn, R. 1998: Using the Ehrenberg Collection. Diatom Res., 13, 273-291
3. Lazarus, D. B. 2000: On the value of paleontological collections. In: Berendsohn, W. G. (ed.), Resource
identification for a biological collection information service in Europe (BioCISE), 33-36.
235
Project ID: 01LC0026 (AlgaTerra Subproject 3)
1.10.2001-30.09.2004
MOLECULAR AND ECOLOGICAL DATA OF TERRESTRIAL DIATOMS
L. Medlin, R. M. Crawford, B. Berszteri, K. Hamann, F. Hinz, and S. Strieben
Alfred Wegener Institute, Foundation for Polar and Marine Research, Am Handelshafen 12, D27570 Bremerhaven
lmedlin@awi-bremerhaven.de; http://www.awi-bremerhaven.de/Biomeer/molekulargenetische-e.html
Key words: diatoms, Hustedt, rRNA, phylogeny, ecological data, culture strains, Nitzschia,
biodiversity informatics
Abstract:
Type localities of the species described by F. Hustedt from terrestrial habitats in Germany and
Northern Europe will be established and re-visited to generate living cultures for deposition as
epitypes in culture collections. Phylogenetic analysis of these epitype cultures will be performed
using molecular techniques. In collaboration with AlgaTerra AT1 a database will be created
including image-based keys to facilitate the identification of terrestrial diatoms.
Introduction. The diatoms (Bacillariophyta) are one of the most successful and ecologically
important groups of microalgae, occurring in both aquatic and terrestrial habitats with over 10,000
described species and potentially many more cryptic species. They are distinctly well known for
their architecturally complex siliceous cell walls (valves and girdle bands) and their unusual
pattern of reduction in cell size of one of the daughter cells following mitosis, among other
features. The intricate designs of their cell wall have been the basis for their classification system
for more than two centuries. In the last fifty years, the diatoms have been routinely used to
provide estimates of ecological perturbation, specifically eutrophication and acid rain. Currently
they are being exploited as proxies for climate change in paleo-environments by looking at salt
tolerances and the changes in the diatom communities in saline lakes and some terrestrial habitats
influenced by salinity.
Germany has had at least three renowned diatomists from the last two centuries – Kützing,
Ehrenberg and Hustedt – who described many hundred new species. Kützing’s material was
distributed to several contemporary scientists and has found its way into museums worldwide.
The Ehrenberg and Hustedt collections remain more or less intact, the former at the Museum für
Naturkunde, Humboldt Universität zu Berlin (see AT2), and the latter at the Friedrich Hustedt
Arbeitsplatz at the Alfred-Wegener-Institute in Bremerhaven, Germany. The Hustedt Collection
will be the basis of this subproject concerning names, types, ecological and molecular data.
Research plan. The Hustedt collection is fully curated [1, 2]. It is currently being catalogued into
an Internet database [2] and will be the focus of our project. The research objectives of subproject
AT3 are:
• To establish the type localities of the type species described by F. Hustedt from terrestrial
habitats from Germany and Northern Europe.
• To return to these localities at the same time of the year to re-establish living cultures of these
earlier described species for deposition in modern culture collections, such as the SAG
(AT4). These new cultures will be designated as epitypes and epitype permanent preparations
will be prepared and deposited in the Hustedt Herbarium.
• To perform phylogenetic analysis of these epitype cultures using an analysis of their rRNA
genes or ITS regions if they are varieties of the same species.
236
•
To create a database, from which an image-based key will be made to facilitate the
identification of these diatoms from both living and fixed, cleaned material using the epitype
culture and the original material in cooperation with AT1. These will be supplemented with
database entries for other important terrestrial diatoms in these collections that were not
described by Kützing, Ehrenberg and Hustedt so that a meaningful assemblage of terrestrial
diatoms will be compiled for identification purposes using our database.
Accordingly, the project is divided into 4 workpackages:
• Databasing of names, pictures and type localities of terrestrial diatoms described by F.
Hustedt.
• The establishment of epitype cultures based on the types and localities from work package 1
and the documentation of dominant feature of each species using both fresh and cleaned
material.
• The reconstruction of the phylogeny (see [3]) of dominant species of terrestrial diatoms from
Germany and Northern Europe based on 18S rRNA sequences derived from the epitype
cultures established in work package 2 and from other cultures of representative diatoms
dominant in our collection from type localities re-sampled throughout Germany and Northern
Europe.
• Construction of an identification key using our database of the photographs of terrestrial
diatoms.
Results. B. Beszteri came to the AWI in July 2001 on a DAAD scholarship. He began
immediately to collect diatoms from one site on the estuary of the Geeste river near the AWI
where Hustedt had made many collections and had described 4 species. B. Beszteri made about
19 collections (16 benthic from different substrates and 3 planktonic) from this site from which he
established 40 cultures. Among the species recovered at the time of the collection he searched for
Nitzschia hoehnkii, N. aestuarii, N. tryblionella f. subconstricta and Diploneis dimorpha which
were described by Hustedt from this site. We are currently awaiting the grow up of existing
cultures to see if we have obtained either of these species in our rough cultures. Preliminary
indications are that we likely have Nitzschia hoehnkii and N. aestuarii in culture collected exactly
at the same location from where they were originally described. We are growing the algae both in
liquid and solid culture to facilitate their manipulation.
References:
1. Simonsen, R. 1987: Atlas and Catalogue of the Diatom Types of Friedrich Hustedt, 1-3. Berlin.
2. Crawford, R. and Hinz, F. 2001: Information on the Collection [http://www.awi-bremerhaven.de/
Research/hustedt3.html]; List of types [http://www.awi-bremerhaven.de/Research/hustedt1.html].
3. Medlin, L. K, Kooistra, W. C. H. F., and Schmid, A.-M. M. 2000: A review of the evolution of the
diatoms – a total approach using molecules, morphology and geology. In: Witkowski, A. and Sieminska, J.
(eds), The origin and early evolution of the diatoms, Kracow.
237
Project ID: 01LC0026 (AlgaTerra Subproject 4)
1.10.2001-30.09.2004
DOCUMENTATION OF CHARACTERS OF TERRESTRIAL ALGAE IN
CULTURE: R-DNA SEQUENCE ANALYSES, MORPHOLOGY AND CRYOPRESERVATION OF REFERENCE STRAINS
T. Friedl1 , D. Hepperle 1 , and Y. van de Peer2
1
Experimentelle Phykologie und Sammlung von Algenkulturen, Albrecht-von-Haller-Institut
für Pflanzenwissenschaften, Universität Göttingen, Untere Karspüle 2, D-37073 Göttingen
2
Evolution Biology, Department of Biology, University of Konstanz, P.O. Box 5560 M618,
D-78457 Konstanz
tfriedl@gwdg.de; http://www.gwdg.de/~epsag/phykologia/sag/sag.htm
Key words: algae, cyanobacteria, rDNA, sequence analyses, phylogeny, morphology,
cryopreservation, culture strains
Abstract:
The project will primarily analyse molecular characters of terrestrial microalgae from cultures,
and will also gather morphological data for those strains that have been sequenced. The results
will be integrated into the comprehensive information system for the unambiguous identification
of these algae and they will be used to explore phylogenetic relationships. In addition, all strains
used in this project will be preserved by cryotechniques and thus made available for further
comparisons and investigations. The project is closely connected with one of the largest culture
collections of algae (SAG, Sammlung von Algenkulturen Göttingen).
Research plan:
Phylogeny research in terrestrial algae, in particular green algae and cyanobacteria from aeroterrestrial habitats:
Molecular characters of terrestrial microalgae (excl. diatoms) will be examined by means of DNA
sequence analyses of ribosomal genes (rDNA) using reference strains. This will help to clarify the
phylogenetic relationships of these algae [1, 2, 3, 4]. A regularly updated gene tree will guide the
user to the sequence data as well as to all other relevant information. The DNA sequence data will
be provided as secondary structure-model based alignments ([5], e.g., in ARB format). The
sequence database will help in identifying algae even from uncultured material (e.g., soil crusts)
or environmental DNA extractions, a feature which is essential for applied research.
Authentic culture and other reference strains form the basis for the documentation of
molecular and morphological characters.
This brings a large culture collection into the focus of modern research. The main source for
culture strains is the “Sammlung von Algenkulturen Göttingen (SAG)” which is one of the
world’s largest living collection of algae [6]. Of the ca. 2100 strains currently in the SAG, 419 are
authentic strains (i.e. represent subcultures of the culture material used for the original taxonomic
description of algal species) of which 369 were isolated from terrestrial habitats. These strains will
be supplemented by additional authentic culture strains, particularly from the culture collection at
Innsbruck, Austria (ASIB, Dr. G. Gärtner), which mainly holds stocks of soil and airborne algae
[7, 8]. Other reference strains used by the project will be carefully selected in collaboration with
the other subprojects of AlgaTerra i.e. AT1 (taxonomic identity, Ehrenberg type) and AT5
(ecological importance as currently known), as well as from other culture collections needed to
represent the diversity of genera of microalgae from aero-terrestrial habitats as best as possible.
238
Further strains may be provided by the projects AlgaTerra AT5, and the BIOTA projects TP05-1
and S05-1.
Preservation of reference strains by cryotechniques
All the information as gathered by the project will be referenced to genetically stable cryopreserved culture material which will be available to the public for further comparisons and
investigations. Although cryopreservation is routinely used in other culture collections, new
protocols for the cryopreservation of microalgae will be developed. The establishment of
cryocultures will ensure the prolonged survival of important reference strains.
Documentation of morphological characters and culture data
All relevant information that is available for the culture strains used in this project (e.g., origin,
optimal culture conditions) will be collected and integrated into the information system.
Morphological features of the strains will be documented, and a collection of image data (with
attached comments that explain morphological characters) will be provided with the information
system.
References:
1. Ben Ali, A., De Baere, R., Van der Auwera, G., De Wachter, R., and Van de Peer, Y. 2001. Int. J. Syst.
Evol. Microbiol., in press.
2. Fewer, D., Friedl, T., and Büdel, B. 2002. Mol. Phylogen. Evol., in press.
3. Friedl, T., Besendahl, A., Pfeiffer, P., and Bhattacharya, D. 2000. Mol. Phylogenet. Evol., 14, 342-352.
4. Friedl, T. in Bhattacharya D. (ed.) 1997: The Origin of Algae and Plastids. Pl. Syst. Evol., [Suppl] 11,
87-101.
5. Van de Peer, Y., De Rijk, P., Wuyts, J., Winkelmans, T., and De Wachter, R. 2000. Nucleic Acid Res.,
28, 175-176.
6. SAG: Sammlung von Algenkulturen an der Universität Göttingen [http://www.gwdg.de/
~epsag/phykologia/epsag.html].
7. Ettl, H. and Gärtner, G. 1995: Syllabus der Boden-, Luft- und Flechtenalgen. Stuttgart.
8. Gärtner, G. 1996. Ber. Nat.-Med. Verein Innsbruck, 83, 45-69.
239
Project ID: 01LC0026 (AlgaTerra Subproject 5)
1.10.2001-30.09.2004
CHARACTERISTIC DATA OF GREEN AND BLUEGREEN AERO-TERRESTRIAL
ALGAE (CHLOROPHYTINA AND CYANOBACTERIA)
W. Reisser and M. Salisch
Institut für Botanik der Universität Leipzig, Johannisallee 21-23, D-04103 Leipzig
reisser@rz.uni-leipzig.de; http://www.uni-leipzig.de/~allbot/
Key words: cyanobacteria, green algae, literature data, culture strains
Abstract:
Whereas information on marine and freshwater algae is one of the standard topics of
phycological textbooks, comparably few data are available for aero-terrestrial taxa, i. e. those
algae which live on and in soil [1, 2], cover surfaces such as rocks and tree barks or which are
part of the aero-plankton. The reasons for this information gap are manifold, but one clear
obstacle for a better understanding of these algae is the difficult access to published information.
Important data are scattered among journals of low citation impact or grey literature and is often
not available in English. This subproject will contribute to overcome this impediment by
assembling available information and making it accessible worldwide.
Research plan:
In order to create the basis for an intensified research on aero-terrestrial algae this project will
focus on:
• the search for, assembly, and processing of data on aero-terrestrial algae (blue-green and
eukaryotic algae excluding diatoms), including translation into English
• axenic cultures of aero-terrestrial algae including their description and taxonomic
identification by classical standard methods and
• the development of an image-based web-interface to chlorophycean aero-terrestrial algae
Progress made:
Currently we are engaged in data mining (morphology, taxonomy, ecology, original descriptions)
of predominantly Russian and Ukrainian sources, translation and processing of data into a
compatible structure. Parallel to this, we are completing data sheets of axenic isolates of aeroterrestrial algae of our algal collection (literature data such as original descriptions,
ecophysiological data, morphological documentation by drawings and photographs).
References:
1. Ettl, H. and Gärtner, G. 1995: Syllabus der Boden-, Luft- und Flechtenalgen. Stuttgart.
2. Wöhler, I., Mensching, A.-K., and Reißer, W. 1999: Algae in Forest soils: Distribution and taxonomic
affiliation. In: Rastin, N., Bauhus, J. (eds), Going underground. Ecological studies in forest soils. Research
Sign Post Trivandran, India, 187-200.
240
241
Appendix
Index of Names
A
C
Aas, G. .................................................................... 44, 46
Adamczyk, J.................................................................80
Ade, M. .......................................................................112
Adeba, P. J..................................................................142
Agerer, R.....................................................................122
Akhtar-Schuster, M...................................................110
Akoegninou, A..........................................................146
Althof, A.....................................................................158
Amann, R......................................................................84
Andres, W. .................................................................146
Anhuf, D .....................................................................130
Arbuckle, T.................................................................196
Audorff, V. ...................................................................38
Austermühle, R..........................................................110
Awiti, A.......................................................................152
Clausnitzer, V.............................................................162
Conrad, R......................................................................82
Crawford, R. M..........................................................238
Cremers, A. B.............................................................196
Cudjoe, A. A..............................................................142
D
Dalitz, H......................................................................155
Dauber, J. ......................................................................74
Davies, A. M. R.........................................................226
Deckert, J. ...................................................................112
Deke, O.................................................................. 92, 94
Deml, G.....................................................210, 214, 220
Dericks, G.....................................................................20
Diedrich, K. ..................................................................76
Dietrich, C..................................................................202
Dojani, S.....................................................................108
Döring, R......................................................................54
Dormann, W.................................................................56
Dressler, S...................................................................146
Dunfield, P....................................................................82
Düring, C......................................................................26
Durka, W.......................................................................64
B
Balkenhol, B.................................................................66
Ballot, A......................................................................184
Barthlott, W................................................................132
Beck, E..........................................................................10
Beck, L........................................................................208
Becker, T.....................................................................110
Behrens, T.....................................................................68
Beierkuhnlein, C................................................... 44, 46
Berendsohn, W. G............................ 16, 224, 228, 230
Bergsdorf, Th.............................................................168
Berndt, R............................................................104, 222
Berszteri, B.................................................................238
Bertke, E. ......................................................................76
Bierschenk, T. ............................................................146
Bleeker, W....................................................................30
Bleher, B. ....................................................................170
Blüthgen, N. ...............................................................208
Bodensteiner, P..........................................................226
Böhning-Gaese, K.....................................................170
Bonkowski, M..............................................................90
Boutros, M..................................................................140
Brake, I........................................................................192
Braker, G.......................................................................82
Braun, G.............................................................100, 128
Braun, U......................................................................218
Braun, W.....................................................................192
Braune, E. ...................................................................114
Brechtel, F..................................................................208
Bresinsky, A.................................................................26
Broll, A..........................................................................56
Buchmann, N................................................................36
Büdel, B.............................................................106, 108
Burhenne, M...............................................................214
Burkhardt, U...............................................................200
Buss, H.-J....................................................................120
Bussmann, R. W........................................................172
E
Eberl, L..........................................................................88
Ebigbo, N. M..............................................................144
Ehlebe, M......................................................................28
Eiseb, S........................................................................112
Engels, C................................................................ 44, 48
Ernst, R........................................................................142
F
Fahr, J..........................................................................144
Falk, T. ........................................................................120
Feige, G.-B. ................................................................178
Feuerer, T....................................................................108
Filser, J........................................................................200
Fischer, A......................................................................76
Fischer, E. ...................................................................158
Fjellberg, A.................................................................200
Franck-Oberaspach, S. L..................................... 92, 94
Franke, T.....................................................................122
Friedl, T.......................................................................240
Frohschammer, S.......................................................142
G
Gebhardt, S...................................................................60
Gerowitt, B. ..................................................................76
Gich, F...........................................................................86
Giere, P........................................................................112
Gliech, M ....................................................................212
Goetze, D. ...................................................................134
Göker, M.....................................................................216
242
Görke, C......................................................................104
Görlich, T......................................................................56
Gräfenhan, T......................................................212, 214
Grau, B........................................................................226
Grau, J. ........................................................................226
Gröngröft, A...............................................................102
Groß, I. ........................................................................192
Grunert, J....................................................................134
Güth, M.........................................................................64
Guttwein, D. ...............................................................192
Jetschke, G....................................................................42
Jobst, A.......................................................................236
Jürgens, N...................................................................110
K
Kahlheber, A..............................................................140
Kahmen, A....................................................................36
Kainz, C......................................................................218
Kalko, E. K. V............................................................144
Kamer, N.......................................................................56
Kersting, P. .................................................................134
Kiefer, S. .....................................................................172
Kier, G.........................................................................132
Kilian, N......................................................................176
Killmann, D................................................................158
Kirchhoff, A...............................................................230
Kirk, M........................................................................120
Klepper, G. ............................................................ 92, 94
Klingenstein, F...........................................................192
Klütsch, C. ..................................................................182
Knight, R.....................................................................120
Koch, F........................................................................112
Koehler, H....................................................................24
Köhler, J......................................................................150
Köhler, W.....................................................................68
König, K......................................................................146
Kossmann, G. ........................................................ 44, 50
Kost, C.........................................................................136
Kosuch, J. ..........................................................142, 164
Kotut, K.......................................................................184
Kouamé, G. N. ...........................................................142
Koulibaly, A...............................................................134
Kowatsch, A.................................................................54
Kraemer, M.................................................................168
Krell, F.-T...................................................................166
Krell-Westerwalbesloh, S. .......................................166
Kreuzer, K....................................................................90
Krieglsteiner, L............................................................26
Krienitz, L...................................................................184
Krug, M.......................................................................120
Kühne, L. ....................................................................160
Küper, W.....................................................................132
Küppers, M.................................................................108
Kürschner, H. .............................................................176
Kusber, W.-H.............................................................234
H
Hachfeld, B.................................................................110
Hadrys, H....................................................................114
Hagedorn, G. ...................................210, 212, 214, 220
Hahn, A..............................................................228, 230
Hahn-Hadjali, K.........................................................146
Hamann, K..................................................................238
Hampicke, U.................................................................54
Hanagarth, W.............................................................208
Harbig, C.....................................................................142
Haszprunar, G. ...........................................................198
Häuser, C. L.......................................................194, 204
Häuser, Ch. L. ............................................................160
Hausmann, A..............................................................206
Heimpel, B..................................................................120
Hein, P.........................................................................176
Held, E...........................................................................52
Hellwig, F. ....................................................................42
Hennenberg, K...........................................................134
Hepperle, D................................................................240
Herrmann, B.................................................................22
Hespelt, S. K.................................................................76
Heyde, K. ......................................................................64
Hille, A........................................................................198
Hinz, F.........................................................................238
Hochkirch, A................................................................56
Höfer, H......................................................................208
Hoffelner, H..................................................................86
Hoffmann, A........................................................28, 112
Hoffmann, I. ...............................................................120
Hoffmann, S. ................................................................28
Holstein, J. ................................................160, 194, 204
Hoppe, J. R. ................................................................188
Hörsch, B. ..........................................................100, 128
Horz, H. P. ....................................................................82
Hou, C.-L....................................................................220
Hubaishan, M. A..............................................176, 182
Hurek, T. .......................................................................78
Hurka, H........................................................................30
Hüttl, R. F. ....................................................................60
L
Lampe, K.-H......................................................192, 202
Langer, E.....................................................................104
Lazarus, D...................................................................236
Lehner, A......................................................................80
Leistner, G. .................................................................142
Linsenmair, K. E......................................136, 140, 142
Loris, K.......................................................................108
Lösch, R........................................................................20
Lötters, S.....................................................................164
Loy, A............................................................................80
Lücking, R. .................................................................108
Ludwig, T...................................................................190
Ludwig, Th.................................................................188
Ludwig, W..................................................................190
I
Ikels, L...........................................................................58
Ingrisch, S...................................................................202
Isaacs, G. .....................................................................120
Isselstein, J....................................................................76
J
Jahn, R................................................................232, 234
Jakupi, A.......................................................................22
Jakupovic, J. ...............................................................228
Jeltsch, F. ....................................................................116
243
M
R
Mahiva, V. ..................................................................166
Marais, E. ....................................................................112
Marbach, B. ................................................................106
Marggraf, R. .................................................................76
Martens, A..................................................................114
Mathenge, S................................................................172
Mathiak, G....................................................................56
Matthäs, U. .................................................................230
Matzner, E. ............................................................ 44, 50
Medlin, L. ...................................................................238
Meier, H. .....................................................................190
Meister, J.....................................................................180
Mennicken, M............................................................104
Mey, W. ......................................................................112
Meyer, F......................................................................208
Miehlich, G.................................................................102
Mies, B........................................................................178
Miller, M. A...............................................................198
Mißkampf, R. ...............................................................56
Mody, K......................................................................136
Morzfeld, J....................................................................72
Mossakowski, D. .........................................................56
Motzer, T....................................................................130
Müller, J........................................................................24
Müller-Motzfeld, G.....................................................56
Münzenberger, B.........................................................60
Musila, W...................................................................155
Mutiso .........................................................................172
Mutke, J.......................................................................132
Rambold, G. ......................................................106, 108
Reimer, L. .....................................................................72
Reineking, B...............................................................118
Reinhold-Hurek, B............................................... 12, 78
Reisser, W.........................................................108, 242
Reuter, A................................................................ 44, 48
Richter, L. ...................................................................190
Richter, O....................................................................114
Richter, S......................................................................28
Riede, K......................................................................202
Riethmüller, A............................................................216
Ritschel, A..................................................................222
Rödel, M.-O................................................................142
Roscher, S...................................................................192
Rotich, D.....................................................................164
Rudolf, V. ...................................................................142
S
Salisch, M..........................................................108, 242
Schaab, G. ...................................................................152
Schareika, N. ..............................................................146
Scheu, S.........................................................................90
Schickhoff, U. ..............................................................52
Schmid, J.......................................................................70
Schmidt, M.................................................................146
Schmiedel, U..............................................................110
Schmitz, U. ...................................................................20
Schneiderat, U............................................................120
Schorr, G.....................................................................142
Schröder, S.................................................................196
Schuhwerk, F..............................................................226
Schultz, C...........................................................100, 128
Schwager, M...............................................................116
Seiberling, S. ................................................................52
Siber, R........................................................................106
Sigl, K............................................................................88
Sinsin, B......................................................................146
Smalla, B.....................................................................228
Solga, A.......................................................................158
Söndgerath, D. ...........................................................114
Spelda, J......................................................................208
Steidle, A......................................................................88
Steinbrecher, R...........................................................152
Steiner, A...........................................................194, 204
Steinhage, V. ..............................................................196
Steinsiek, P.-M.............................................................22
Stoll, P. T. .....................................................................92
Stoll, P.T. ......................................................................94
Straßl, L.......................................................................155
Strieben, S...................................................................238
Strohbach, B...............................................................110
Strohbach, M..............................................................110
Strunz, G. ....................................................................152
Suhling, F....................................................................114
Surma, A.....................................................................230
Szarzynski, J...............................................................130
N
Nashr, A. K.................................................................182
Naumann, C. M.................................................150, 182
Neßhöver, C........................................................... 44, 46
Neumann, K................................................................146
Newman, D.................................................................166
Njeri, R........................................................................172
Njoroge, G. .................................................................172
Nuppenau, E.-A.........................................................120
O
Oberprieler, Ch..........................................................180
Oberwinkler, F.......................104, 210, 216, 220, 222
Odat, N..........................................................................42
Onyango, J..................................................................172
Ott, S............................................................................108
Overmann, J..................................................................86
P
Perner, J.........................................................................40
Pernthaler, A.................................................................84
Pernthaler, J..................................................................84
Petersen, A.................................................................102
Piepenbring, M..................................................210, 220
Pillukat, A...................................................................226
Pivarci, R....................................................................134
Plötner, J.....................................................................112
Porembski, S...............................................................134
Purtauf, T. .....................................................................74
T
Teske, K. .....................................................................234
Tews, J.........................................................................116
Thies, C.........................................................................70
244
Thiombiano, A...........................................................146
Thünen, T. ....................................................................78
Traoré, D.....................................................................134
Triebel, D...........................................................210, 218
Trusch, R.....................................................................206
Tscharntke, T. ..............................................................70
Tsichrintzis, F.............................................................228
Tute, C...........................................................................76
Weimann, J...................................................................28
Weiß, M......................................................................216
Weiss, M............................................................212, 218
Weisser, W. W...................................................... 34, 40
Wendt, H.....................................................................112
Werner, D...................................................................120
Wessolek, G..................................................................58
Westram, R.................................................................190
Wiegand, K...................................................................68
Wirth, V. .....................................................................108
Wissel, C.....................................................................118
Wittig, R......................................................................146
Wittmann, D...............................................................196
Woas, S. ......................................................................208
Wöllecke, J. ..................................................................60
Wolters, V.............................................................. 12, 74
Wurst, C......................................................................208
Wytrykush, C. ..............................................................40
U
Uhlig, M......................................................................112
Uhlmann, E.................................................................104
Uster, D.......................................................................155
V
van de Peer, Y............................................................240
Vasiliou, A..................................................................224
Veith, M.............................................................142, 164
Verhaagh, M...............................................................208
Vidal, S..........................................................................72
Vierk, R.........................................................................56
Visser, U.......................................................................68
Vogel, M............................................................100, 128
Voglmayr, H...............................................................216
Vohland, K.................................................................112
Voigtländer, K..............................................................66
X
Xylander, W.................................................................66
Z
Zdero, C......................................................................228
Zedda, L. .....................................................................108
Zeller, U. .....................................................................112
Zhang, L. .......................................................................78
Ziebarth, A....................................................................56
Zimmermann, M..........................................................56
Zimmermann, R...........................................................52
Zizka, G.......................................................................146
W
Wagner, M....................................................................80
Walsh, M. ...................................................................152
Warnecke, F..................................................................84
Weber, B. ....................................................................106
Wegmann, M..............................................................142
245
BIOLOG-Coordinators
BIOLOG-Europe
Prof. Dr. Volkmar Wolters
Department of Animal Ecology
Justus-Liebig-University Giessen
Heinrich-Buff-Ring 26-32 (IFZ)
D-35392 Giessen
Phone: ++49-(0)641-9935700
volkmar.wolters@allzool.bio.uni-giessen.de
assisted by
Christine Kugelstadt
Christine.Kugelstadt@allzool.bio.uni-giessen.de
Prof. Dr. Barbara Reinhold-Hurek
Laboratorium für Allgemeine Mikrobiologie
Universität Bremen, FB 2
Leobener Strasse NW2
D-28359 Bremen
Phone: ++49-(0)421-2182370
breinhold@uni-bremen.de
BIOLOG-Africa
West Africa:
Prof. Dr. Eduard Linsenmair
Lehrstuhl für Tierökologie und Tropenbiologie
Universität Würzburg, Biozentrum
Am Hubland
D-97074 Würzburg
Phone: ++49-(0)931-8884351
ke_lins@biozentrum.uni-wuerzburg.de
assisted by
Dr. Minnattallah Boutros
boutros@biozentrum.uni-wuerzburg.de
East Africa:
Prof. Dr. Clas Naumann
Zoologisches Forschungsinstitut und Museum Alexander Koenig,
Adenauerallee 160
D-53113 Bonn
Phone: ++49-(0)228-9122201
c.naumann.zfmk@uni-bonn.de
assisted by
Dr. Jörn Köhler
j.koehler.zfmk@uni-bonn.de
246
Southern Africa:
Prof. Dr. Norbert Jürgens
Botanical Institute
University of Hamburg
Ohnhorststr. 18
D-22609 Hamburg
Phone: ++49-(0)40-42816-260
norbert.juergens@uni-koeln.de
assisted by
Dr. Ingo Homburg
ihomburg@botanik.uni-hamburg.de
Biodiversity Informatics
Prof. Dr. Walter Berendsohn
Department of Biodiversity Informatics and Laboratories
Botanic Garden and Botanical Museum Berlin-Dahlem
Freie Universität Berlin
Königin-Luise-Str. 6-8
D-14191 Berlin
Phone: ++49-(0)30-83850143
wgb@zedat.fu-berlin.de
assisted by
Alexandra Vasiliou
a.vasiliou@bgbm.org
Contact BIOLOG Programme
DLR e.V.
Project Management Organization
on behalf of the Federal Ministry of
Education and Research
Environmental Research and Technology
Godesberger Allee 119
D-53175 Bonn
Dr. Lothar Quintern
Phone/Fax: ++49-(0)228-81996-20/-40
lothar.quintern@dlr.de
Dr. Arndt Wüstemeyer
Phone/Fax: ++49-(0)228-81996-34/-40
arndt.wuestemeyer@dlr.de
247
Jakob Fahr