conferenceseries.com
574th Conference
10th Asia-Paciic Biotech Congress
July 25-27, 2016 Bangkok, Thailand
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Day 1
Bio Asia-Paciic 2016
Page 29
Tracks: 7 & 9
Day 1 July 25, 2016
Track 7: Pharmaceutical Biotechnology
Track 9: Nano science & Nanotechnology
Session Chair
Jagdish Singh
North Dakota State University, USA
Session Co-chair
Mohd Basyaruddin Abdul Rahman
Universiti Putra Malaysia, Malaysia
Session Introduction
Title: Molecularly modiied insulin for controlled delivery from triblock copolymers
Jagdish Singh, North Dakota State University, USA
Title: Ultra-sensitive drug delivery platform to detect minuscule electrical activity of glioma cell populations
Paulo R F Rocha, Max Planck Institute for Polymer Research, Germany
Title: Murine models of dengue and their utility for drug and vaccine testing
Alonso Sylvie, National University of Singapore, Singapore
Title: Optimization of palm based nanoemulsions for drug delivery systems
Mohd Basyaruddin Abdul Rahman, Universiti Putra Malaysia, Malaysia
Title: Targeted inhibition of transcription factor STAT3 for the prevention and treatment of cancer
Gautam Sethi, National University of Singapore, Singapore
Title: In silico, In vitro and cytotoxicity investigations of biphenylalanine and its derivatives as potential hiv1 gp120 attachment inhibitors
Teow Chong Teoh, University of Malaya, Malaysia
Title: Omics approach in the study of natural hybridization in Nepenthes species
Hoe-Han Goh, Universiti Kebangsaan Malaysia, Malaysia
Title: 2Gy, 6Gy, 8Gy, 10Gy may be some ideal fractional doses with the better biological response
Hong Zhao, Wuhan University, China
Page 30
Jagdish Singh et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Molecularly modiied insulin for controlled delivery from triblock copolymers
Jagdish Singh, Mayura Oak and Divya Sharma
North Dakota State University, USA
T
he objective of the present work was to develop a delivery system for controlled release of insulin at basal level based on chitosanzinc-insulin complex incorporated into poly (lactic acid)-poly (ethylene glycol)-poly (lactic acid) (PLA-PEG-PLA, 4500 Da)
thermosensitive polymer. In vitro release proile of insulin from the delivery system was studied in phosphate bufered saline, pH
7.4 (PBS). A signiicant decrease (p<0.05) in the initial burst was observed from the formulation containing chitosan-zinc-insulin
complex compared to zinc-insulin, chitosan-insulin and insulin alone. Additionally, the release of insulin was complete with minimal
secondary burst. he polymeric formulations containing chitosan-zinc-insulin complex showed a long-term controlled release (~84
days) of insulin. he in vivo absorption and bioactivity of insulin released from the delivery systems were studied in the streptozotocininduced diabetic rat model. Chitosan-zinc-insulin complex signiicantly (P<0.05) reduced the initial burst release of insulin from the
polymeric delivery system in comparison to zinc-insulin or insulin alone in vivo in rat. he delivery system released insulin for ~3
months in biologically active form with corresponding reduction in blood glucose levels in diabetic rats. he delivery systems were
biocompatible both in vitro and in vivo and were non-immunogenic. he results indicate that the chitosan-zinc-insulin complex
incorporated in the thermosensitive polymeric delivery system can be used as an alternative to the conventional daily basal insulin
therapy.
Biography
Jagdish Singh is a Professor and Chairman of the Department of Pharmaceutical Sciences at North Dakota State University School of Pharmacy and a Fellow of
American Association of Pharmaceutical Scientists (AAPS) and Fellow of Association of Biotechnology and Pharmacy. His research efforts focus on the mechanistic
studies for developing and testing novel delivery technologies to deliver biotechnologically derived molecules. He has published over 170 peer-reviewed papers
and 300 abstracts.
jagdish.singh@ndsu.edu
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 31
conferenceseries.com
Paulo Roberto Ferreira da Rocha et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Ultra-sensitive drug delivery platform to detect minuscule electrical activity of glioma cell populations
Paulo Roberto Ferreira da Rocha and Dago M de Leeuw
Max Planck Institute for Polymer Research, Germany
D
etecting minuscule electrical activity of cell populations is a major challenge to electrophysiologists. To overcome such challenge,
we built a highly sensitive measuring setup. he setup comprises a biosensor based on a metal/Si/SiO2/interdigitated gold
electrode. Interferences were minimized with adequate shielding. To validate the extreme sensitivity of the measuring system, we
investigated the electrical activity of large populations of two cell lines known to be electrically quiescent. he cell lines studied were
the human cervix carcinoma cell line, HeLa and C6 glioma cells. HeLa cells are supposed to be electrically quiet as they don’t originate
from the brain or any other electrically excitable tissue. However, due to the high sensitivity of our measurement system, even the
HeLa cells demonstrate luctuations of their basal current level, which is much higher in amplitude than the background acquisition
noise. he low frequency analysis of the HeLa cells reveals clear current luctuations of about 3 pA. Measurement of glioma cells
subtle electrical activity is typically hampered by the high background noise. Here, we detect glioma electrical behavior without any
physical disruption or interference into their physiology. he current noise analysis, performed with the sensing system determined
that the glioma cell activity is primarily caused by the opening of voltage gated Na+ and K+ ion channels and can be eiciently
abolished using speciic pharmacological inhibitors. We present here a unique tool to study the electrophysiological properties of
large cell populations as an in vitro reference for tumor bulks in vivo.
Biography
Paulo Roberto Ferreira da Rocha has completed his PhD from University of Algarve, Portugal, in 2014. Since 2014, he has been a Postdoctoral Researcher at Max
Planck Institute for Polymer Research in Mainz, Germany.
paulorfdrocha@gmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 32
Alonso Sylvie, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Murine models of dengue and their utility for drug and vaccine testing
Alonso Sylvie
National University of Singapore, Singapore
D
engue (DEN), the most serious arthropod-borne viral disease, manifests as a mild febrile illness to life-threatening hemorrhage
and vascular leakage. he development of an animal model has long been recognized as a major roadblock in understanding
dengue pathogenesis and validating therapeutic and prophylactic strategies prior to clinical stages. Humans and mosquitoes are the
natural hosts for dengue virus (DENV) in its urban cycle. While most immunocompetent mice are not susceptible to infection from
all four DENV serotypes, type I & II interferon deicient mice may develop asymptomatic transient viremia and in few cases, relevant
clinical manifestations. Furthermore, it is possible to reproduce the antibody-dependent enhancement of disease severity (ADE)
in this mouse model. In this conference, we will present the various mouse models of dengue currently available in our laboratory,
ranging from asymptomatic transient viremia to lethal models associated with vascular leakage or liver damage, two relevant clinical
manifestations. We will also present our unique models of ADE mediated by maternal antibodies acquired during both gestation and
breastfeeding. his mouse models platform has made our group visible to the Dengue research scene and has attracted a signiicant
number of collaborations, licensing and research service contracts with both industry partners and public institutions for evaluating
the in vivo eicacy of prophylactic and therapeutic candidates.
Biography
Alonso Sylvie has completed her PhD in University Claude Bernard Lyon I, France and pursued her Postdoctoral studies at the Pasteur Institute of Lille, France
and Cornell University, NY, USA. She has established laboratory at NUS, Department of Microbiology and Immunology. She is also a Member of the Immunology
program at the Life Sciences Institute, NUS. Her main interests lie in host-pathogen interactions with a focus on Dengue, Tuberculosis and Enterovirus 71.
She has published more than 60 peer-reviewed papers in reputed journals and has been serving as an Editorial Board Member of PLOS ONE and Frontiers in
Immunotherapies and Vaccines.
sylvie_alonso@nuhs.edu.sg
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 33
Mohd Basyaruddin et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Downsizing antifreeze proteins to antifreeze peptides from Antarctica inhabitants
Mohd Basyaruddin, Abdul Rahman and Azren Aida Asmawi
Universiti Putra Malaysia, Malaysia
S
ome cold-adapted organisms are able to synthesize antifreeze protein (AFP) to enable them to survive in subzero environment.
he ability of AFP to reduce the ice damage in cells has attracted interest in many applications for example medical and agriculture
sectors. However, high capital cost in producing AFP may hamper them to be resourcefully used in industrial biotechnology. Lately,
peptides that mimic the parent proteins have been designed and synthesized for many purposes. AFP type I from Antarctic yeast
Glaciozyma antarctica and shorthorn sculpin ish, Myoxocephalus scorpius were downsized to functional several α-helix antifreeze
peptides. he short peptide segments derived from both AFPs gave high antifreeze activity and ice recrystallization inhibition.
Molecular modeling ofα-helix antifreeze peptides on ice surface showed the straightforwardness of the peptides is related to the high
antifreeze activity. hese synthetic antifreeze peptides could be a new hope in food preservation and cryopreservation technology as
it could inhibit the growth of ice crystals.
Biography
Mohd Basyaruddin Abdul Rahman is currently the Professor of Chemistry at Universiti Putra Malaysia. He has received his PhD in Catalysis Chemistry in 1999 at
the University of Southampton, England. He has developed skills in catalysis at synchrotron radiation in Daresbury and Grenoble, Protein Engineering at Osaka
University and Structural Biology at the University of Edinburgh. His research areas include biocatalysis, protein chemistry, nanobiotechnology and computational
chemistry. He is among the pioneer Chemists in this country to synergize experimental results with theoretical insights. He has published more than 170 cited
papers and 300 proceedings in the wide ield of biocatalysis. To date, he has iled more than 20 patent applications in Malaysia and 10 internationally. He has
supervised and co-supervised more than 30 PhD and 35 MSc postgraduate students. He has won Young Scientist recognition from various bodies including ACS,
IUPAC and IAP. He is currently the Founding Chairman of the Young Scientists Network and also being elected as the Fellow of the Academy of Sciences Malaysia.
basya@upm.edu.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 34
Gautam Sethi, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Targeted inhibition of transcription factor STAT3 for the prevention and treatment of cancer
Gautam Sethi
National University of Singapore, Singapore
S
TATs comprise a family of cytoplasmic transcription factors that transmit signals, mediate intracellular signaling usually generated
at cell surface receptors and transmitted to the nucleus. Numerous studies have demonstrated constitutive activation of STAT3
in a wide variety of human tumors, including blood malignancies (leukemias, lymphomas and multiple myeloma) as well as solid
tissues (such as head and neck, breast, lung, gastric, hepatocellular and prostate cancers). here is a strong evidence to suggest that
aberrant STAT3 signaling promotes development and progression of human cancers by either inhibiting apoptosis or inducing cell
proliferation, angiogenesis, invasion and metastasis. However, the development of novel drugs for the targeting STAT3 that is both
safe and eicacious remains an important scientiic and clinical challenge. We will present the data that shows that novel small
molecule inhibitors of STAT3/JAK2 pathway can suppress the expression of genes involved in cancer initiation and promotion both
in vitro and in vivo.
Biography
Gautam Sethi has completed his Postdoctoral training at University of Texas MD Anderson Cancer Center and joined Department of Pharmacology, Yong Loo
Lin School of Medicine, National University of Singapore in 2008 as an Assistant Professor and was promoted to Associate Professor in 2015. The focus of his
research over the past few years has been to elucidate the mechanisms of activation of oncogenic transcription factors such as NF-kB/STAT3 by carcinogens
and inlammatory agents and the identiication of novel inhibitors of these proteins for prevention of and therapy for cancer. From traditional Chinese and Indian
medicinal plants, his group has identiied numerous small molecules that can suppress various pro-tumorigenic signaling cascades involved in cancer initiation and
promotion. The novel indings of his research work have so far resulted in more than one ifty scientiic publications in high impact factor peer-reviewed journals
and several international awards. He currently serves as an Academic Editor for prestigious PLOS ONE journal and ad-hoc Reviewer for several other international
journals.
gautam_sethi@nuhs.edu.sg
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 35
Teow Chong Teoh et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
In silico, in vitro and cytotoxicity investigations of biphenylalanine and its derivatives as potential HIV-1
gp120 attachment inhibitors
Teow Chong Teoh, Hussin A Rothan, Mohammed Rizman Idid and Md Arman Hossain
University of Malaya, Malaysia
I
n this study, molecular modeling and docking of gp120-CD4 protein complex crystal structure (PDBID: 1g9n) was used to design
a novel attachment inhibitor, biphenylalanine and its derivatives (BPAs) that target HIV-1 gp120 and prevent its binding to CD4
on host cell. Molecular docking results by AutoDock Vina showed that L-biphenylalanine has highest binding probability than
D-biphenylalanine and L-methyl-biphenylalanine and exhibited low negative docked energy. he CD4 capture ELISA experiments
indicated that L-biphenylalanine has an IC50 at 200 µM. BPAs were non-toxic up to 400 µM in the Vero cell cytotoxicity test. In
addition, BPAs fulill “the Lipinski rule of ive” criteria as good drug candidates.
Biography
Teow Chong Teoh is currently a Senior Lecturer of Bioinformatics program, Institute of Biological Sciences, Faculty of Science, Malaysia. He has obtained his BSc
and MSc degrees in Biochemistry and PhD degree in Computational Chemistry from University of Malaya. He has 10 years of research experience in molecular
modeling and simulation for chemical and biological systems. He has published numerous ISI/WOS journals, presented research papers in conferences and
is holding the Chinese and US patents and has reviewed a number of research manuscripts. He has received research grants from University of Malaya and
Malaysian Government for his research projects. He is also the Secretary of Malaysian Society of Marine Sciences.
ttchong@um.edu.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 36
Hoe-Han Goh et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Omics approach in the study of natural hybridization in Nepenthes species
Hoe-Han Goh, Muhammad Mu’izzuddin Zulkapli and Wan Mohd Aizat Wan Kamaruddin
Universiti Kebangsaan Malaysia, Malaysia
T
he Nepenthes genus consists of carnivorous plants that have evolved intricate pitcher formation at the tip of their leaves for
nutrient acquisition from insect trapping. Previous studies reported several proteins present in the pitcher luid, including aspartic
proteases (nepenthesin I and II) and pathogenesis related proteins (β-1, 3-glucanase, class IV chitinase and thaumatin like protein)
which are enzymatically stable albeit exposed to external environment. We are interested in understanding the efect of natural
hybridization on the expression of enzymes in the pitcher luids of Nepenthes. N. ampullaria, N. ralesiana and their natural hybrid,
Nepenthes x hookeriana were chosen as the model due to the distinct feeding habits of N. ampullaria as a detritivore which feeds on leaf
litter, while N. ralesiana and Nepenthes x hookeriana remain carnivorous. To identify novel proteins in the pitcher luids will require
transcript information for the inference of peptide sequences through proteomics informed by transcriptomics (PIT) approach.
PacBio isoform sequencing (Iso-Seq) provides unprecedented full length transcriptome proiles of all three species as reference
databases for protein identiication. Comparative transcriptomic analysis was performed through orthologous clustering of predicted
peptide sequences. he hybrid, Nepenthes x hookeriana has the highest number of predicted proteins, whereby more proteins were
shared with N. ralesiana than N. ampullaria. his is consistent with previous genetic study and morphological observation. We are
currently investigating allele speciic gene expression and whether novel transcripts are generated as a consequence of hybridization.
Biography
Hoe-Han Goh was graduated from the University of Shefield, UK. He has started his irst academic position at the Institute of Systems Biology, Universiti Kebangsaan
(National University) Malaysia in Nov 2011. He has trained himself on the applications of NGS by attending courses and workshops on NGS data analysis held at BGIShenzhen and TGAC UK. Since then, he has established a plant functional genomics research group with a focus on crop improvement and molecular exploration of
tropical plant species using NGS and functional genomics approaches. In Jun 2014, he was appointed as Head of Plant Biotechnology Centre.
gohhh@ukm.edu.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 37
Hong Zhao et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
2Gy, 6Gy, 8Gy, 10Gy may be some ideal fractional doses with the better biological response
Hong Zhao, Fuxiang Zhou and Yunfeng Zhou
Wuhan University, China
Objective: To study the radiation response of the cancer cells to diferent fractional dose irradiation and explore the ideal fractional
dose.
Methods: Observe the variability of cell cycle, cell proliferation, apoptosis, DNA damage and repair and the expression of related
proteins of human breast cancer cell line MDA-MB-435 which was administrated by diferent dose of X-ray irradiation (0Gy, 2Gy,
4Gy, 6Gy, 8Gy, 10Gy, 12.5Gy, 15Gy, 20Gy). Flow cytometry was used to observe the rate of apoptosis and cell cycle arrest, CCK8
method and colony formation assay were used to examine cell proliferation, the expression of DNA damage and repair and other
related proteins were detected with western blotting.
Results: he apoptosis of cell, G2/M phase proportion and the inhibition of cell proliferation were increased ater irradiation with
the increment of the exposure dose; those changes had an obvious increasing trend. he expression of apoptosis suppression protein
Bcl-2 was decreased ater diferent dose of irradiation. On the contrary, the apoptosis related protein Bax, Caspase-9 and BID were
elevated ater irradiation compared to control group (0Gy), the expression of Bax in groups 6Gy, 8Gy, 10Gy, 12.5Gy, 15Gy were
signiicant higher than those in other groups, the expression of Caspase-9 were higher in the groups 2Gy, 4Gy, 6Gy, 8Gy than other
groups, the expression of BID in the groups 2Gy, 4Gy, 6Gy were higher than others. G2/M phase arrest was related to the increase of
ATR/ATM AND the reduction of CHK1, CyclinB1 and CDC25B. DNA damage and repair related protein Ku80 was increased ater
the irradiation in the groups 2Gy, 4Gy, 10Gy,15Gy, 20Gy compared to control group, in contrast, the expression of it was decreased in
the groups 6Gy, 8Gy, 12.5Gy. he expression of the Rad51 was increased ater irradiation but the level of the groups 2Gy, 8Gy, 10Gy
were lower than that of other groups.
Conclusion: X-ray irradiation will increase cell apoptosis, make cell block in G2/M phase and decrease the cell proliferation. But we
can come to the conclusion that 2Gy, 6Gy, 8Gy and 10Gy may be the ideal fractional dose, as those can promote cell apoptosis, leads
to DNA damage and inhibit its repair.
Biography
Hong Zhao is currently a MD student of the Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University. She has completed her Master’s
degree from the same department and has published 1 paper as co-author in reputed journal. Her major ields of interest are metabolomics and radiosensitivity of cancer.
vivisugar@foxmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 38
Tracks: 4 & 8
Day 1 July 25, 2016
Track 4: Medical Biotechnology
Track 8: Agricultural Biotechnology
Session Chair
Petr Malý
BIOCEV Research Center, Czech Republic
Session Co-chair
Lei Yang
Wuhan University, China
Session Introduction
Title: Biotech and innovative breeding for the new Agri-Food System CGIAR Research Programs
(CRPs)
Philippe. Ellul, Agropolis International, France
Title: Targeting IL-23/Th17 pro-inlammatory axis by novel non-immunoglobulin protein binders
with an immunosuppressive potential
Petr Malý, Institute of Biotechnology CAS, BIOCEV Research Center, Czech Republic
Title: Applications of stem cell therapy in regenerative medicine in Asian countries
Kampon Sriwatanakul, Asian Institute of Regenerative Medicine, Thailand
Title: Genetic engineering for sustainable improvement of oil palm
Ahmad Parveez Ghulam Kadir, Malaysian Palm Oil Board, Malaysia
Title: Suppression of TPP1 resulted in telomere dysfunction and enhanced radiosensitivity in
cancer cells regardless of telomerase status
Lei Yang, Wuhan University, China
Title: Effects of low level laser (diode 830 nm) therapy (LLLT) on human bone regeneration
Mohammad Nazrul Islam, Shaheed Suhrawardy Medical College Hospital, Bangladesh
Title: Glucose metabolism related enzymes may become some ideal targets to increase
radiosensitivity of cancer cell
Hong Zhao, Wuhan University, China
Title: VCPA, a novel synthetic derivative based on α-tocopheryl succinate, sensitize gastric cancer
cells to doxorubicin-induced apoptosis via the mitochondrial pathway
Han Wu, Zhongnan Hospital of Wuhan University, China
Title: UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells
Xiaojia Gao, Zhongnan Hospital of Wuhan University, China
Bio Asia-Paciic 2016
Page 39
Philippe Ellul et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Biotech and innovative breeding for the new Agri-Food System CGIAR Research Programs (CRPs)
Philippe Ellul, P Gardiner and W Powell
Agropolis International, France
C
GIAR’s mission is to design and apply innovative science to convert the 21st century’s grand challenges into prosperous and more
equitable opportunities for people of the developing world. Food and nutrition security, biodiversity, climate change, natural
resources management are globally identiied (G20) as strategic priorities for both developed and developing countries. With its
15 research Centers (IRRI, CIMMYT, CIAT, CIP, etc...) collaborating in 12 CGIAR Research Programs (CRPs) and 3 platforms
(Genebank, Big Data & Genetic Gain), CGIAR is involved in a reform process initiated in 2008 and entering in phase 2 for 201722 (Strategic Research Framework, 2015). Eight of the new CRPs are Agri-Food System programs involved in the improvement of
staple crops (maize, wheat, rice, potato, Sorghum, etc...), livestock and ish. Most of these programs are collaborating in South Asian
integration countries for increased impact. hese programs are engaged in genomic analysis as maize and wheat with the seed of
discovery project, RICE collaborating with CAAS and BGI for re-sequencing 3,000 lines of rice (and very soon 10,000) or RTB (Root
Tubers and Banana) re-sequencing 1,200 cassava accessions. Accurate prediction of phenotype from genotype through genomicsassisted breeding is now feasible in crops or livestock. Millions of genotyping data are produced in high throughput genotyping
platforms and used by pre-breeders in parallel with the breeding information phenotyping and ield trials necessarily compiled in a
modern and standardized breeding management system. Innovative computational methods are being designed and shared to support
the management, collation, curation, visualization and analysis of multivariate, complex data sets to improve the identiication of
causative connections between genotype and phenotype and between phenotype and landscape. For rice agro-ecosystems, emerging
concepts and technologies will form a key lagship (FP5) of the new RICE AFS full proposal aiming to accelerate genetic gain by
capitalizing on breeding material, knowledge, and tools developed from in a irst phase (GRiSP 2011-16). Novel biotech approaches
including genome editing and blue-sky research such as systems biology and C4 rice project for developing a rice plant switched to
C4 photosynthetic engine and adapted anatomical attributes, will be implemented. Analytical pipelines are being developed through
Centers and CRP partnerships such as the Genetic Gains platform and the Genomic and Open-source Breeding Informatics Initiative
(GOBII) to accelerate genetic gain. An emerging related-issue is the human capacity to design and implement such innovative
approaches. Pre-breeders skilled in quantitative genetics, mapping analysis, genomics, biotechnology or breeding populations design
are being trained and incorporated into the new AFS programs. We will describe how CGIAR breeding programs are evolving to
deliver multiple beneits based on diverse biotech innovations.
Biography
Philippe Ellul holds a PhD in Plant Biotechnology from the University Polytechnic of Valencia, Spain. As a Researcher, he was involved in EU and Spanish national
and regional projects (1997-2005) focused on vegetables’ improvement for developing crops with increased salt and drought tolerance, resistance to biotic stresses,
modiied architecture, parthenocarpic or long shelf life fruits. As an Assistant Professor, he taught genetics, plant biotech & breeding and supervised master’s degree
and PhD theses for agricultural engineers in Spain, Colombia, Brazil and Ecuador. He has worked for the private sector (2005-13) managing international R&D
collaborations and designing innovative approaches combining genomics, molecular and cellular biology to support breeding programs. He has joined CGIAR in 2013
and is actively involved in the monitoring of the programs and the design of the second portfolio (2017-22). He has published international papers and patents.
p.ellul@cgiar.org
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 40
Petr Maly, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Targeting IL-23/Th17 pro-inlammatory axis by novel non-immunoglobulin protein binders with an
immunosuppressive potential
Petr Maly
Institute of Biotechnology of the CAS, v.v.i., BIOCEV Research Center, Czech Republic
I
L-23 receptor-mediated signaling has recently been closely associated with development of several chronic autoimmune diseases
such as psoriasis, psoriatic arthritis, inlammatory bowel disease and multiple sclerosis. Recently we generated novel IL-23 receptor
antagonists (called REX ligands) derived from scafold of albumin-binding domain (ABD) that exhibited immunosuppressive control
over IL-23-driven ex vivo expansion of primary human h-17+ T-cells. Due to small size, excellent tissue penetration and selfrefolding activity, these binders represent a valuable non-immunoglobulin alternative for development of topically-administrated
anti-psoriatic biologicals. As the structure of IL-23/IL-23R complex is unavailable and a precise mode of interaction remains
unknown, designing more eicient IL-23 inhibitors is cumbersome. Following our concept, we generated a novel class of binding
proteins targeting p19 subunit of human IL-23 cytokine. hese unique proteins, called ILP binders, were selected from high-complex
ABD-derived combinatorial library in combination with ribosome display. From 214 clones analyzed by ELISA, Western blot and
DNA sequencing, 53 provided 35 diferent sequence variants that were further characterized. Using in silico docking in combination
with cell-surface competition binding assay we identiied a group of inhibitory candidates that substantially diminished binding of
recombinant p19 to the IL-23 receptor on human monocytic THP-1 cells. Several found p19-blocking variants inhibited IL-23-driven
expansion of IL-17-producing primary human CD4+ T-cells. hus, these novel binders represent unique IL-23-targeted probes useful
for IL-23/IL-23R epitope mapping studies and could be used for designing novel p19/IL-23-targeted anti-inlammatory biologics.
Biography
Petr Maly is the Head of Laboratory of Ligand Engineering at the Institute of Biotechnology, Czech Academy of Sciences in Vestec near Prague, Czech Republic.
He has studied at the Department of Biochemistry, Faculty of Science, Charles University in Prague and completed his Doctorate at the Institute of Molecular
Genetics, ASCR in Prague. He has completed his Postdoctoral fellowship at the Department of Pathology and Howard Hughes Medical Institute, The University of
Michigan Medical School, USA, in the laboratory of Professor John B. Lowe where he has published several substantial papers related to in vivo role of mammalian
glycosyltransferases. Since 1998 to 2005 he was a Research Group Leader at the Institute of Molecular Genetics in Prague. He has also worked as Visiting Scientist at
the Department of Biochemistry and Molecular Biology of Oklahoma Center for Medical Glycobiology, College of Medicine, the University of Oklahoma, USA. He was a
Participating Investigator of Consortium for Functional Glycomics, USA (2001-2008) and Member of Editorial Board (2001-2005) and Editor (since 2003) of the Czech
Journal “Biologicke listy”. Since 2008, he has been working on development of combinatorial protein libraries derived from small protein scaffolds and construction of
novel high-afinity protein binders with therapeutic and diagnostic potential.
petr.maly@ibt.cas.cz
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 41
Kampon Sriwatanakul, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Applications of stem cell therapy in regenerative medicine in Asian countries
Kampon Sriwatanakul
Asian Institute of Regenerative Medicine, Thailand
S
tem cell therapy is an emerging form of healthcare that ofers signiicant potential to improve the practice of medicine. Despite
the large number of cell therapy are in clinical trials, relatively few have made it to the market in the United States. In Asia,
Korea seems to develop cell therapy products more rapidly than other countries. In Asian countries, we attempt to promote the
applications of stem cell therapy in regenerative medicine. he PRP (Platelet Rich Plasma) kit is currently in the process of seeking
FDA approval as a Class II medical device. Clinical trials were conducted to demonstrate the eicacy and safety of this innovative kit
in the treatments of facial rejuvenation and mild to moderate cases of knee joint osteoarthritis (OA). he results demonstrated the
consistent efects in stimulating cell proliferation and tissue regeneration. here were statistically signiicant diferences regarding
the general appearance, facial skin irmness, sagging and wrinkle states before each PRP procedure and 3 months ater the last PRP
procedure. he intra-articular injection of hyaluronic acid (HA) plus PRP could strongly rescue the meniscus tear and cartilage
breakdown. he combination of HA and PRP can synergistically promote cartilage regeneration and inhibit OA inlammation. hese
studies might ofer advanced treatments for facial rejuvenation and alternative OA treatments based on regenerative mechanisms.
Biography
Kampon Sriwatanakul is an internationally recognized pioneer of stem cell therapy, received MD degree and PhD degree from Mahidol University where he had an
academic career for more than 35 years. He has also received training in Clinical Pharmacology from University of Leicester, UK and University of Rochester, USA.
Apart from publishing more than 40 publications in international journals, he has spearheaded a number of important research and development activities related to
stem cell technology in Thailand, including setting up of cord blood and tooth cell banking.
drsrikul@gmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 42
conferenceseries.com
Ahmad Parveez Ghulam Kadir et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Genetic engineering for sustainable improvement of oil palm
Ahmad Parveez Ghulam Kadir, O Abdul Rasid, M Y Abdul Masani, A M Dayang Izawati, B Bahariah, M Siti Masura, A Nur Hanin, W S Wan Nur Syuhada, A R
Nurfahisza, I Nor Fakhrana, F H Lim and S Ravigadevi
Malaysian Palm Oil Board, Malaysia
O
il palm is the most productive vegetable oil crop that can be used either as edible foodstuf or industrial feedstock. Systematic
progress in conventional breeding, application of good agriculture practices among others has allowed the oil palm industry
to obtain a solid footing in South East Asia, particularly in Malaysia and Indonesia. However, to remain competitive in the long
term, diversiication of research into proitable areas, such as palm biotechnology is a necessity. Biotechnology ofers tremendous
opportunities for further improvement of oil palm yields. he idea is to deploy appropriate biotechnology tools to obtain highest
yielding trees, with good quality oil and minimal environmental footprint. In this regard, MPOB made a major breakthrough in
sequencing the genome of oil palm, which allowed the identiication of genes inluencing important agronomic traits. Conversion
of these discoveries into molecular diagnostic assays will also help improve breeding eiciency. Apart from conventional breeding,
MPOB also has an active tissue culture program to clone the highest yielding palms. Uniformity of planting materials via cloning,
ofers the opportunity to increase yields up to 30%. However, abnormalities observed in a small percentage of the clones, has restricted
large scale commercialization. Nonetheless, it has opened up opportunities for deciphering the epigenome of oil palm to understand
molecular basis of clonal abnormality. Developing transgenic technologies has also been an important component in MPOB’s R&D
eforts to further diversify the use of palm oil. he main focus has been to develop high oleate oil palm for the high value oleochemical
industry. Biotechnology eforts towards increasing oil palm production will contribute to world food security.
Biography
Ahmad Parveez Ghulam Kadir is the Director of Advanced Biotechnology and Breeding Centre at the Malaysian Palm Oil Board (MPOB). He has completed his PhD
on Plant Genetic Engineering at the Universiti Putra Malaysia under Asian Development Bank’s Scholarship. He was also appointed by the Honorable Minister of
Natural Resources and Environment of Malaysia to Chair the Genetic Modiication Advisory Committee (GMAC) under the National Biosafety Board from 25th May
2010 to 24th May 2016.
parveez@mpob.gov.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 43
Lei Yang et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Suppression of TPP1 resulted in telomere dysfunction and enhanced radiosensitivity in cancer cells
regardless of telomerase status
Lei Yang1, Weiguang Qiang2 and Yunfeng Zhou1
Wuhan University, China
2
Soochow University, China
1
R
adiotherapy is one of the major therapeutic strategies in can¬cer treatment and identifying new factors that predict radioresistance
could be of great value in the treatment of cancers. Telomere homeostasis is now emerging as an efective and important factor
in modulating cellular sensitivity to ionizing radiation. he telomere-binding protein TPP1, an important component of the shelterin
complex at mammalian telomeres, is an important regulator of telomere homeostasis. In this study, we investigated the role of TPP1
expression in regulating cellular radiosensitivity and telomere homeostasis in both telomerase positive (HCT116) and alternative
lengthening of telomere (ALT) cell lines (U2OS). We found that TPP1 deletion lead to a signiicant increase of radiosensitivity
to X-rays in both telomerase positive (HCT116) and alternative lengthening of telomere (ALT) cell lines (U2OS). TPP1 mediated
radiosensitization was correlated with increased telomere dysfunction and apoptosis rate ater IR exposure. Moreover, TPP1 deletion
slowed down the repair kinetics of total DNA damage and telomere dysfunction induced by ionizing radiation. Together, our study
demonstrated that TPP1 plays a vital role in telomere maintenance and cellular response to ionizing radiation and may be a potential
target in the radiotherapy of cancer regardless of telomerase status.
Biography
Lei Yang has completed his PhD/MD in 2014 from Wuhan University, China. He has worked in the Department of Radiation Oncology & Medical Oncology at Zhongnan
Hospital, Wuhan University for 1 year. His main research ield is the role of telomere and telomerase in anti-cancer therapy.
yfzhouwhu@163.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 44
Mohammad Nazrul Islam, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Effects of low level laser (diode 830 nm) therapy (LLLT) on human bone regeneration
Mohammad Nazrul Islam
Shaheed Suhrawardy Medical College Hospital, Bangladesh
T
issue healing is a complex process that involves both local and systemic responses and the healing process of bone is much slower
than that of sot tissues which is a great challenge of medical science. he use of Laser herapy (LLLT) for wound/bone healing
has been shown to be efective by modulating both local and systemic response by enhancing cellular & mitochondrial ion exchange,
bone mineralization, nitric oxide formation, lymphatic circulation, osteoblast proliferation, efects on osteoblast gene expression,
osteoclast inhibition (prevents bone mineral resorption) and by bone engratment on synthetic materials. he result observed here is
that the bone density in the laser treated group, at fracture site, at the end of 3rd week is equivalent to the bone density of control group
at the end of 4th week of incidence. Treatment with 830 nm diode laser has substantially reduced the fracture healing time as well as
improved the quality/quantity of callus formation of the patient; thus accelerates bone regeneration and enhance fracture healing.
Laser biostimulative efects on bone could be a new dimension for bone regeneration which signiicantly reduce healing period,
lessen cost of treatment and enhance patient compliance.
Biography
Mohammad Nazrul Islam has completed his MBBS degree from Dhaka University and later MSc (BME) from Gonobiswabidyalaya, Bangladesh. He is the founding Head of
Biomedical and Medical Biotechnology Department of Shaheed Suhrawardy Medical College and Hospital, Bangladesh. He has published papers in reputed professional,
national and international forum, journals and continues academic research work at Shaheed Suhrawardy Medical College, Institute and Hospital since 2007.
abbirr@gmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 45
Hong Zhao et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Glucose metabolism related enzymes may become some ideal targets to increase radiosensitivity of
cancer cell
Hong Zhao, Fuxiang Zhou and Yunfeng Zhou
Wuhan University, China
Objective: To establish a radioresistant cell line of cancer cells and investigate the proteins related to glucose metabolism and to ind
out the diferences between them.
Methods: Established radioresistant cell line (435R) of breast cancer cell line MDA-MB-435 through exposed to continuous X-ray
radiation (2Gy a day for ive days a week and a total dose of 60Gy), the radiation resistance of cells was detected by colony formation
assay. Enzymes that related to the glucose metabolism of the cells were detected at the level of transcription and translation levels. he
changes of the metabolite concentrations were detected with the special kits.
Results: he radioresistant cell line 435R was established ater continuous X-ray irradiation and validated by colony formation assay.
Expression of the GLUT1 in the radioresistant cell 435R was signiicantly higher than that in its parental cell 435S. he glucose
metabolism related enzymes PKM2, LDHA, PDHA and IDH1 in the 435R were signiicantly higher than those in the 435S. he ATP
levels were signiicantly higher in 435R compared to its parental cells.
Conclusion: Glucose uptake of the radioresistant cell was increased compared to its parental cells. Aerobic glycolysis and oxidative
phosphorylation in the radioresistant cell were higher than its parental cells. he glucose metabolism related enzymes may become
some ideal targets of adjustment of the radiosensitivity of cancer cells.
Biography
Hong Zhao is currently a MD student of the Department of Radiation and Medical Oncology, Zhongnan Hospital, Wuhan University. She has completed her Master’s degree
from the same department and has published 1 paper as co-author in reputed journal. Her major ields of interest are metabolomics and radiosensitivity of cancer.
vivisugar@foxmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 46
Han Wu, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
VCPA, a novel synthetic derivative based on α-tocopheryl succinate, sensitize gastric cancer cells to
doxorubicin-induced apoptosis via the mitochondrial pathway
Han Wu
Zhongnan Hospital of Wuhan University, China
G
astric cancer (GC) remains one of the most common malignancies, especially in East Asia. Although surgical resection is the main
method in curing cancer, chemotherapy remains an important treatment to avoid tumor recurrence and metastasis. Doxorubicin
(DOX) is one of the most efective and widely used chemotherapeutic agents for the treatment of various malignancies. And it is also
widely used to treat GC in combination regimens with etoposide, cisplatin and 5-luorouracil. However, the dose-dependent adverse
efects signiicantly limit its eicacy. Previous researches demonstrated that dysregulation of apoptosis pathway is a fundamental,
common aberration adapted by of most cancer cells to survive, proliferate and metastasis. Hence, we have recently proposed a novel
synthetic derivative VCPA as a chemosensitive agent, which could enhance the eicacy of DOX and thereby reducing the DOX doses.
GC cell lines SGC7901, AGS, MKN28 and MGC803 were used to investigate the cytotoxicity under VCPA treated. he IC50 doses for
48 hours were 19.96 uM, 10.67 uM, 11.30 uM and 14.01 uM, respectively. he inhibition of VCPA was displayed in a dose and time
dependent manner. Pretreatment of GC cells with VCPA at IC50 for 24 hours signiicantly enhanced the inhibition of DOX both in
vitro and in vivo. Even the DOX-resistant GC cells displayed apoptosis ater pretreatment. his drug combination strategy caused
rapid production of ROS in GC cells. And the response of GC cells to the drug correlated with induction of pro-apoptotic protein Bax,
inhibition of anti-apoptotic protein Bcl-2, activation of caspase-3 and inally promoting PARP cleavage. Collectively, these results
imply that VCPA, a novel synthetic derivative based on α-tocopheryl succinate, potentiates DOX induced apoptosis in GC cells and
is in sight to reduce patient adverse response to DOX. One of the potential mechanisms by which the pretreated combination therapy
has synergistic cytotoxic efects against gastric cancer may be through the mitochondrial apoptosis pathway.
Biography
Han Wu is currently pursuing her PhD from Zhongnan Hospital of Wuhan University, Wuhan, China. She has completed her MD in Oncology from the Second Military
Medical University, Shanghai, China. She has performed studied on primary hepatocyte isolation and culture, establishment of liver disease model such as DEN-induced
hepatocellular carcinogenesis in mouse and rat, DMN-induced acute hepatic failure in rat and tumor metabolism. She has successfully applied for the National Natural
Science Foundation of China grant (NO: 31371440) as the principle person. Presently her main research ield is the drug resistant in abdominal tumors. She has published
two papers as co-author in reputed journals.
wuhan_whu@163.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 47
Xiaojia Gao et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
UBE2D3 gene overexpression increases radiosensitivity of EC109 esophageal cancer cells
Xiaojia Gao and Yunfeng Zhou
Zhongnan Hospital of Wuhan University, China
R
adiotherapy is widely used in adjuvant approaches for esophageal cancer (EsC) to reduce local recurrence and improve survival.
However, the overall 5-year survival was about 17% over the past few decades. herefore, ind an efective way to improve the
efect of radiotherapy is vital. We have been engaged in this work for 15 years. Not only radiosensitivity is associated with a collection
of associated proteins and telomere, we found, but also telomerase. Telomerase is, furthermore, regulated by post-translational
modiications of the rate limiting catalytic subunit hTERT. We had used yeast two-hybrid method to identify UBE2D3 encoding an
E2 ligase which is a principle hTERT-interacting protein and inhibition of UBE2D3 expression attenuates radiosensitivity of MCF7 human breast cancer cells by increasing hTERT expression and activity and we found that UBE2D3 is negatively correlated with
hTERT expression and is a positive prognostic factor in EsC. To verify a possible contribution of UBE2D3 to tumor cell radiosensitivity,
esophageal squamous carcinoma cells (EC109 cells) were transfected with the expression plasmid encoding UBE2D3 and stable
transfectants were subsequently established. UBE2D3-overexpressing cells exhibited an increased incorporation of radiosensitivity, to
further investigate the mechanism; the CCK-8 assay was used to conirm cell proliferation, which showed that UBE2D3 downgrades
EC109 cells propagation. Moreover, cell cycle distribution was examined by low cytometry, UBE2D3 overexpression in EC109 cells
causes prolonged G1 arrest ater IR exposure on the contrary G2/M shortened. We, then, detected the protein expression about
ATM/ATR-Chk2 pathway by western blotting, which in UBE2D3 over-expressing cells showed decrease ater irradiation. And
overexpression of UBE2D3 decreases the protein level of hTERT relative to the control cell line. Subsequently, we immunoprecipitated
with anti-hTERT antibody followed by immunoblotting with anti-ubquitin antibody to examine the in vivo role of UBE2D3 in
ubiquitination of hTERT. Overexpression of UBE2D3 caused a clear and dramatic increase in the amount of ubiquitinated hTERT
species ater 2 hours of speciic proteasome inhibitor MG132 treatment, which points out hTERT may be degraded by the proteasome
pathway. To determine whether this change can inluence the telomere length, we used real-time PCR to test the relative telomere
length and result suggests that overexpressed UBE2D3 is negative correlation with telomere extension. In conclusion, these indings
suggest that UBE2D3 may be a potential target in the radiotherapy of EsC.
Biography
Xiaojia Gao has completed his Bachelor's degree from Hubei University of Traditional Chinese Medicine in 2013. He is currently pursuing his Master's degree in radiationguided gene therapy of cancer at Wuhan University, China. He has published 2 papers in national journals and presented results of his research at a national conference.
yfzhouwhu@163.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 48
conferenceseries.com
574th Conference
10th Asia-Paciic Biotech Congress
July 25-27, 2016 Bangkok, Thailand
p
ho
rks
Wo
Day 2
Bio Asia-Paciic 2016
Page 51
Workshop on Stem Cell Technology
Day 2 July 26, 2016
Asean Society of Regenerative Medicine, Thailand
Session Introduction
Title: Introduction
Dr. Vichit Punyahotra, Senior Lecturer of School of Anti Aging and Regenerative Medicine, Thailand
Title: Update on stem cell biology
Petcharin Srivatanakul, Laboratory Director of Bio MSC Co., Ltd., Thailand
Soontaree Petchdee, Department of Large Animal and wildlife Clinical, Sciences, Faculty of Veterinary
Medicine, Kasetsart University, Thailand
Title: Application of immune cell-based therapy for cancer and aging
Bor-Yu Tsai, NAVI BioTheraputics, Inc
Title: Growth factor complex
Win-Ping Deng, Director of Stem Cell Research Center of Taipei Medical University, Taiwan
Title: Basic concept of biology medicine
William van Ewijk, Director of Placenta Research Foundation, Netherlands
Peter Klaus Cremer, Edith-liebergeal-institut, Germany
Title: Regenerative medicine in clinical practice
Daryl Turner, World Authority and Specialist in Thyroid symptoms And Treatment
Viroj Vong, American Board of Hair Restoration Surgery, USA
Surachai Liamthong, President of VitaStem Co,.Ltd.,Thailand
Page 52
Soontaree Petchdee, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Therapeutic applications of dental pulp stem cells in veterinary medicine
Soontaree Petchdee
Kasetsart University, Faculty of Veterinary Medicine, Thailand
S
tem cells treatment has been a considerable research interest over the last decade. Several stem cell types have been studied as the
possible candidates to restore the structure and function of damaged tissues and organs. he dental pulp stem cells (DPSCs) have
shown potential for their use as an alternative resource in regenerative medicine. DPSCs have mesenchymal stem cell-like (MSC)
qualities, including the capacity for self-renewal and multi-lineage diferentiation potential. In this study, we demonstrate the potential
applications of DPSCs as a tool to repair damaged tissues and organs. Diseases related to chronic inlammation such as ischemic heart
diseases, osteoarthritis, tendonitis, ocular injury and chronic wounds have been investigated through experimental and clinical trial
design to clarify the use of DPSCs treatments. Transplantation of DPSCs provided a good option in terms of tissue regeneration
and remodelling. his study suggested that DPSCs might provide a new perspective for translational medicine. However, important
points in DPSCs biology, such as homing and immune-regulation require further study of underlying mechanisms to support the
application of DPSCs in the future.
Biography
Petchdee has completed her PhD from University of Glasgow at 2009. She later worked as a lecturer at faculty of veterinary medicine, Kasetsart University. She
has published more than 10 papers in reputed journals and has been an editorial board member of many reputed journals.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 53
Win-Pin Deng et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Growth factor complex
Win-Pin Deng1 and Kampon Sriwatanakul2
1
Taipei Medical University, Taiwan
2
Asean Society of Regenerative Medicine, Thailand
T
here have been enough published evidences suggesting that beneicial efect of stem cell may not be restricted to cell or tissue
regeneration alone, but also due to their paracrine efects. Stem cells can secrete potent combination of trophic factors that
mediate the molecular composition of the environment to elicit responses from resident cells. he trophic factors secreted by stem cell
are usually known as growth factors and cytokines. Growth factor is a naturally occurring substance capable of stimulating cellular
growth, cell proliferation, tissue healing and cellular diferentiation. hey are very important for signaling a variety of cellular processes.
For more than 20 years, growth factors have been used in the treatments of blood diseases and cardiovascular diseases. hese include
platelet-derived growth factor (PDGF), transforming growth factor (TGF), tumor necrosis factor alpha (TNF alpha) and vascular
endothelial growth factor (VEGF). Platelets also contain growth factors that are responsible for stimulating tissue regeneration,
repairing damage tissues and stimulation of the wound healing processes as well. Platelets also release large amount of growth factors
including PDGF, VEGF, TGF, epithelial growth factor (EGF) and ibroblast growth factor (FGF). Cytokines and chemokines are small
proteins secreted by many cell types. hey are important signaling messengers mediating cell communication and activating other
cells through binding to speciic receptors. Cytokines also play an important role in the immune system, in which the chemokines
mainly function as chemo attractants. hey also coordinate and regulate the biological processes such as cell growth and tissue repair.
Our growth factor complex (GFC) in combination of tissue engineering has been developed as the innovative medical device. he
goal of tissue engineering is to assemble functional constituents that restore, maintain or improve damaged tissues and organs. he
system consists of GMP grade centrifuge and second-generation platelet-rich plasma (PRP) collection tubes. In this presentation,
clinical studies aiming to assess the eicacy and safety of this innovative device for the treatments of low back pain and osteoarthritis
are being reported. Rapid pain relief (within one week) was being achieved in more than 85% of the tested subjects. Decreased pain
and improved function were also reported by most patients. No signiicant adverse efects were being reported. We are conducting
further studies in sport injuries, in the aesthetic applications and the treatments of diabetic wounds. he results of these pilot studies
are very encouraging. We believe that GFC medical device is a revolutionary and non-invasive treatment that uses the patients’ blood
samples to stimulate the natural healing process for a variety of orthopedic conditions and athletic injuries.
Biography
Win-Ping Deng obtained the doctorate in cancer biology at the Harvard University in 1993. In 2000, he joined the Faculty of Oral Medicine at the Taipei Medical
University where now he is the Director of Stem Cell Research Center. Dr. Deng pioneered a new research for combining stem cell and molecular imaging to study
the cancer therapy and tissue regeneration.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 54
Tom Kho et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Regenerative medicine in clinical practice
Tom Kho and Kampon Sriwatanakul
Asian Society of Regenerative Medicine, Thailand
R
egenerative technologies to boost up innate repair processes and restore damage organs and tissues is becoming a new era in
health care. A regenerative medicine model would consist of scalable production and standardized applications of clinical grade
biotherapies. Regenerative medicine aims to ofer solutions for many incurable diseases. he need for innovative technology is due
to the rising number of chronic diseases alicting an aging global population. It has been estimated that by 2020 chronic diseases
in particularly cardiovascular diseases, diabetes, brain diseases, respiratory conditions and cancers will cause more than 70% of all
death globally. People over 60 years-old will sufer from disabilities ranging from visual and hearing loss, dementia or osteoarthritis.
here are several methods employed in regenerative medicine. hese technologies may incorporate stem cell transplantations, tissue
engineering and uses of growth factors and appropriate cytokines. Our current approaches employ platelet rich plasma (PRP), growth
factor complex, autologous stem cell therapy and immunotherapy for rejuvenation, aesthetic applications and for the treatments of
osteoarthritis, sport injuries and diabetic wounds. he allogeneic mesenchymal stem cell (MSC) therapy can be given intravenously
and home to sites of tissue injury and can accelerate the tissue repair processes. Several clinical trials and ongoing studies indicated
their potential uses in various degenerative diseases. During the last decade, many published data suggest that MSCs possess the
innate capacity to home to site of inlammation, including tumor microenvironments. his homing response can be applied by
using MSCs a cellular delivery vehicle to deliver anti-cancer agent directly to tumor. Stem cell function declines with age in both
human and experimental animals. Aging leads to functional decline of hematopoietic stem cells, including alterations of self-renewal
and cell diferentiation. It has been hypothesized that aging of stem cell is the underlying cause of impaired tissue homeostasis
as well as cancers in aged individuals. Recently, the aspect of aging of the stem cell niche on altered phenotypes associated with
aged stem cell is more clearly understood. New data were presented on the role of sestins in regulating metabolism and therefore
the development of aging-related pathologies. Diferent status of metabolism of the cell and its niche could lead to activation or
inactivation of the signaling pathways of metabolism and consequently inluence cell fate regulation. More evidences indicate that
stem cells have distinct metabolism compared to diferentiated cell and the unique metabolism property of stem cell is important
for their maintenance. It is of great signiicance for studies of energy metabolism in aging as well as carcinogenesis. he interactions
between telomeres, telomerase, DNA damage response and senescence as well as their relations to cancer and aging are also needed
to be explored much further.
Biography
Tom Kho is a leading stem cell researcher especially in the ield of mesenchymal stem cell technology. He has developed sevrral innovative stem cell products
currently available in the global market. He has contributed signiicantly to several research and development projects currently on going in Thailand.
drsrikul@gmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 55
Petcharin Srivatanakul, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Update on stem cells biology
Petcharin Srivatanakul
Bio MSC Co. Ltd., Thailand
S
tem cells are special type of cells, which can be found almost in each type of tissue and through entire life span in multicellular
organism. heir main functions are to provide tissue development, homeostasis and reparation of tissue damage. Stem cells are
deined as cells that have the capacity to self-renewal, multipotency/pluripotency, clonality, and are divided into embryonic stem cells
and adult stem cells. ESCs and iPSCs are very similar .Pluripotent stem cells hold great promise in the ield of regenerative medicine.
Because they can propagate indeinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and
liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. hey have a capacity
to undergo chromosomal changes and develop into cancerous cells. It has been shown that transplantation of ESCs into animal
produces teratomas or teratocarcinomas. Hematopoietic stem cells (HSCs) are the stem cells that give rise to all the other blood cells
through the process of haematopoiesis. hey are derived from mesoderm and located in the red bone marrow.he hematopoietic
tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent
progenitors. HSCs are a heterogeneous population. Mesenchymal Stem Cells (MSCs) are a group of adult stem cells naturally found in
the body from a variety of tissues such as bone marrow, peripheral blood, umbilical cord, placenta, adipose tissue, dental pulp etc. that
diferentiate along several mesenchymal lineages . Adult stem cells are undiferentiated cells found in numerous tissues throughout
the body that divide to replenish dying cells and regenerate damaged tissues. On the other hand, there are signiicant diferences in
the proliferation and diferentiation abilities, and in harvesting procedures among these MSCs.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 56
Bor-Yu Tsai, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Application of immune cell-based therapy for cancer and aging
Bor-Yu Tsai
NAVI-Biotherapeutics Inc., Thailand
T
he immune cells play the central roles to detect and destroy abnormal cells may prevent the process of cancer and aging. Cellular
senescence, a state of irreversible cell cycle arrest, can be triggered by diferent mechanism including oncogene activation, telomere
erosion, DNA damage, oxidative stress and chemotherapy. It is well known that systemic or organ-speciic accumulation of senescent
cells results in aging and aging-related disease. Moreover, senescence associated with the disruption of the tissue microenvironment
enhances the development of a pro-oncogenic environment, principally via the secretion of senescence-associated pro-inlammatory
factors. Recent studies demonstrated that clearance of p16Ink4a-positive senescent cells delays the development of aging-associated
disease. herefore, senescent cells become an attractive target for anti-aging therapy. Both innate and adaptive immune cells have
great capacity to eliminate senescent and malignant cells accumulated in tissue, organ and in peripheral circulation. Senescent cells
communicate with their microenvironment by secreting many of kinds of pro-inlammatory cytokines, chemokines, growth factors
and proteases. his condition is called senescence-associated secretory phenotype (SASP).he SASP recruits and activates immune
cells to clear senescent cells, which may promote tissue repair and remove potentially tumorigenic factors in the body. For example,
NK cells, which belong to a component of innate immune system could recognize senescent cells through NKG2D ligand-receptor
interaction and utilize granule exocytosis to kill senescent cells. Induction of cellular senescence is also a therapeutic strategy in
cancer treatment by applying systemic pro-senescent stress such as chemotherapy and ionizing radiation. hese kinds of therapies
generate persistent senescent cells that might damage surrounding tissues via the SASP and then cause long-term side efects of
cancer treatment such as accelerated aging. herefore, using immune cells that could clear senescent cells as an adjuvant therapy
might have beneicial efects in cancer patients who received pro-senescent therapy.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 57
Vichit Punyahotra, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Stem cells and regenerative medicine in the Asian region
Vichit Punyahotra
Mae Fah Luang University, Thailand
I
n the past decade, stem cell research and regenerative medicine have received much attention by research institutes and companies
based in the Asian region. However, there are very few companies that can deal with global development in the biotechnology
market. For the time being, government support in driving the growth of this particular industry is still limited. Private or institutional
investors are not yet convinced to take a risk on a company developing a product using science that few people understand. Only a
handful of countries have developed regulatory framework for regenerative medicine approval. Japan and South Korea are probably
the only two countries in Asia that can ofer regenerative medicine products and technologies having approval from regulatory
authorities. Degenerative diseases including cardiovascular diseases, stroke, diabetes, Alzheimer’s disease, liver cirrhosis, bone and
joint diseases and kidney failure are diicult to treat. he existing conventional treatments are expensive and not very efective.
Regenerative medicine could help cut down on the costs and provide more efective outcome. he idea behind regenerative medicine
is to replace or regenerate cells or organs that have been damaged. In theory, regenerative medicine could someday grow a new liver
or replace damaged brain cells, treat spinal cord injuries, heart diseases, brain diseases and more. Much of regenerative medicine
relies on stem cells, which are the building blocks of organs and tissues. Stem cells and their progenitors ofer great hope for treating
diseases by providing an unlimited source of cells for repairing or replacing damaged tissues and organs. Hematopoietic stem cell
transplantation is the application of bone marrow, peripheral blood and cord blood stem and progenitor cells in order to establish
marrow and immune functions in patients with a variety of cancers and non-malignant disorders. hese include leukemia, lymphoma,
multiple myeloma, bone marrow failure and thalassemia. Mesenchymal stem cells (MSCs) are stromal cells that have the ability to
self-renew and can diferentiate into more than hundred tissues and organs. MSCs can be isolated from a small aspirate of bone
marrow or adipose tissue from the patient and can be harvested from baby teeth and umbilical cord tissues. his type of stem cell
is easy to be cultivated and expanded in vitro. hey are able to moderate immune response which make them good candidate for
regenerative medicine especially to be used for allogeneic transplantation. he understanding of stem cell aging in the pathological
tissue and malignant tumor is highly relevant for future development in biomedicine. Moderation of physiological and pathological
processes might open new perspective to restore organ function lost by disease or physical trauma or to overcome pathophysiological
conditions.
Biography
Vichit Punyahotra is currently a senior lecturer of School of Anti-aging and Regenerative Medicine of Mae Fahluang University, President of Health Foundation and
President of Institute of Asean Integrative Medicine. He is also an American Board certiied plastic surgeon having been trained in Cornell University, U.S.A. His
areas of expertise include alternative medicine, holistic medicine, anti-aging medicine and energy medicine.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 58
Peter Klaus Cremer, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Basic concepts of biological medicine
Peter Klaus Cremer
Edith Liebergeld Institute, Germany
L
ife and growth are a continuous balancing out of charges from “plus” and “minus” at the cell membrane. Everything which lives
and grows is based on the so-called “Schumann resonance frequency of 7.83 Hz. Humans are embedded and fed back to the daynight rhythm, the weekly rhythm and the monthly rhythm. he circadian clock measures time in periods which are approximately 24
hours long for example during waking and sleeping. he recipient of all of these rhythms which represent a signal for the organism is
the epiphysis and pituitary gland. hese two endocrine glands are the main controlling organs in the human organism including the
whole glands and hormone system. Both play an ever greater role today in people getting not enough sleep. he cities are illuminated
too strong at night by artiicial light sources, this destructive efect our health because the day-night rhythm is constantly being
disturbed. Light (photons) have the efect that no further melatonin is produced so we can no longer sleep well. A chronic lack of sleep
over time leads to chronic immunosuppression. his makes the basis for degenerative diseases, particularly cancer, cardiovascular
diseases and depression. Human beings need 8 hours of sleep per day. here may be individual deviations amongst the population
of plus or minus 2 hours per day. However, 6 hours is the absolute minimum for an adult. Cell therapy as a therapeutic concept
was irstly performed by Dr. Paul Niehans, a surgeon and endocrinologist, in 1931. he so called “fresh cell therapy” involved the
transfer of animal cell material into the human organism by means of an injection. One usually uses organs from sheep fetuses and
from young animals. he injected organ suspension is not based on either an unspeciic stimulator efect nor is based on hormones.
he efect is strictly organ-speciic trophotropic and is fundamentally based on substitution of cell fragments in an active form
which activate the molecular repair capability. Organs consist of cells whereby every cell is a tiny organism in itself. Every one of the
billions of body cells performs their metabolism according to a cybernetically controlled plan. he immediate command to do this
comes from the nucleus of the cell (DNA, RNA). Embryonic stem cells or cells from young animals do not have any speciic immune
orientation. hey do not produce any allergic reactions. However, there are also some immediate allergic reactions occurred, if an
injection is made when there is a known allergic tendency. hrough xenogeneic transfer of animal cell materials, the organism is
persuaded to implement the biochemical life of the cells in the individual energy types. he scientiic foundations of cell therapy are
based on more than a thousand papers published in German literature. It is necessary to have more studies done elsewhere in order
to assess the eicacy and safety of the xenogeneic stem cell transplantation.
Biography
Claus-Peter Cremer is a bio-physicist, cell therapist and head of research at Edith Liebergeld Institute, Germany. Claus-Peter Cremer had extensive studies at
Gottingen and Frankfurt Institutes. His research interests range from energy medicine, cell therapy, peptide therapy and homeopathy.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 59
conferenceseries.com
574th Conference
10th Asia-Paciic Biotech Congress
July 25-27, 2016 Bangkok, Thailand
c
tii
en
Sci
&
cks
Tra
s
act
str
Ab
Day 3
Bio Asia-Paciic 2016
Page 61
Tracks: 1, 6, 10, 11 & 14
Day 3 July 27, 2016
Track 1:Environmental Biotechnology
Track 6: Industrial Biotechnology
Track 10: Biotechnology Applications
Track 11: Genetic Engineering
Track 14: Waste water treatment using Bio Techniques
Session Chair
Sarabjeet Singh Ahluwalia
General Shivdev Singh Dewan Gurbachan Singh Khalsa College, India
Session Co-chair
Zurina Zainal Abidin
Universiti Putra Malaysia, Malaysia
Session Introduction
Title: Scale-up of microbial oil production using oleaginous ilamentous fungi
Zhanying Zhang, Queensland University of Technology, Australia
Title: Decolorization of textile dyes Basic Red 46 and Reactive Black 5 by Staphylococcus
epidermidis MTCC 10623
Sarabjeet Singh Ahluwalia, General Shivdev Singh Dewan Gurbachan Singh Khalsa
College, India
Title: Pathogen detection by iber optic microluidic biosensor
Zurina Zainal Abidin, Universiti Putra Malaysia, Malaysia
Title: Bioiltration with Cyperus alternifolus for nutrient removal and water reuse in suburban
area
Yiu Fai Tsang, The Hong Kong Institute of Education, Hong Kong
Title: Metabolomics approach for biomarker discovery of Epinephelus fuscoguttatus infected with
vibriosis
Syarul Nataqain Baharum, Universiti Kebangsaan Malaysia, Malaysia
Title: Exploring active microbial community of anaerobic digestion systems by 454
pyrosequencing
Xiaoying Helen Lu, Technological and Higher Education Institute of Hong Kong, Hong Kong
Title: Morphological identiication of rod lactic acid bacteria using scanning electron
microscope and image analysis
Hoda Elzeini, Cairo University, Egypt
Title: Odour Treatment in Potable Water using Bioiltration with Ozone Addition
Yiu Fai Tsang, The Hong Kong Institute of Education, Hong Kong
Title: Methodology for the valuation and negotiation of the biotechnology products with
intangibles
Dora Garcia Delgado, Center for Genetic Engineering and Biotechnology of Cuba, Cuba
Title: Age at natural menopause and osteoporosis: A genomic appraisal
Mahima Gulabani, University of Delhi, India
Page 62
Zhanying Zhang et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Morphology control of ilamentous fungus Mucor plumbeus for improved microbial oil production
Zhanying Zhang and Ian M O’Hara
Queensland University of Technology, Australia
M
icrobial oils have important applications in producing high value fuels and nutraceutical products. In recent years, microbial oil
production by oleaginous ilamentous fungi has attracted increasing interest because fungi have relatively high growth rates are
able to use a variety of carbon sources and have relatively low harvesting cost compared to heterotrophic cultivation of microalgae.
Many studies on microbial oil production by ilamentous fungi have been focused on low-cost substrates such as lignocellulosic
biomass. However, research on morphology control is limited, which is challenging in cultivation of ilamentous fungi and critical
towards process scale-up and commercialization. We have previously identiied an oleaginous ilamentous fungus Mucor plumbeus
for microbial lipid production. Cultivations of this fungus at diferent conditions and scales have shown the variation in fungal
morphological forms. Diferent inoculation and process control strategies have been proposed and preliminary tested in order to
control the morphological forms of this fungus suitable for reactor cultivation and process scale-up.
Biography
Zhanying Zhang has obtained his PhD in 2008 from the University of Adelaide, Australia. He is currently a Research Fellow at Queensland University of Technology,
Brisbane, Australia. He is an experienced Researcher in fermentation technology, bioprocess optimization and scale-up, biomass pretreatment and bioreinery. He
has published more than 30 papers in peer-reviewed journals and 4 book chapters. He also has 3 patent applications in processing lignocellulosic biomass for the
production of fermentable sugars.
jan.zhang@qut.edu.au
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 63
Sarabjeet Singh Ahluwalia et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Decolorization of textile dyes Basic Red 46 and Reactive Black 5 by Staphylococcus epidermidis MTCC 10623
Sarabjeet Singh Ahluwalia and Anamika Pokharia
General Shivdev Singh Dewan Gurbachan Singh Khalsa College, India
A
critical situation has aroused due to increased discharge of wastewater containing considerable extent of azo dyes from textile
dyeing and manufacturing industries, which requires prior treatment, before being released in order to prevent contamination
of natural water courses and ground water. In the present study, Staphylococcus epidermidis MTCC 10623 isolated from contaminated
site was explored for the decolorization and degradation of two diferent types of azo dyes such as Basic Red 46 (BR 46) and Reactive
Black 5 (RB 5), extensively used in textile processing industries. S. epidermidis has shown remarkable decolorization potential (99.6
and 97%) for the selected dyes at the concentration of 100 mg/L under optimized conditions (pH: 9.0; temp: 40 and 35 °C) ater 6 and
24 hours incubation respectively. Moreover, addition of carbon (glucose: 0.3%) and nitrogen (ammonium sulfate: 0.1%) source as cosubstrates enhanced the decolorization potential of S. epidermidis. Further, decolorization of BR 46 and RB 5, was achieved 99.7 and
97.2% decolorization within 6 and 24 hours respectively with immobilized S. epidermidis over polyurethane foam. Biodegradation
analysis of metabolites formed ater bacterial treatment by UV-Vis and FTIR spectrophotometer conirms the degradation of these
dyes. Phytotoxicity assay of extracted metabolites on Triticum aestivum and Vigna radiata showed good germination rate and growth
of radical and plumule demonstrating the non-toxic nature of treated dye solution and suggest that this bacterium is the eicient azo
dye degrading bacteria and has practical application in biological treatment of dye-polluted wastewater streams.
Biography
Sarabjeet Singh Ahluwalia has completed his PhD from Thapar Institute of Engineering & Technology, (Presently Thapar University), Patiala. He is an Assistant
Professor in Biotechnology at General Shivdev Singh Dewan Gurbachan Singh Khalsa College, Punjab, India. He has published more than 21 papers in reputed
journals and has been serving as an Editorial Board Member of repute. He has three patents to his credit. He has reviewed a number of research papers/
manuscripts.
sarabjeetbiotech@rediffmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 64
Zurina Zainal Abidin et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Pathogen detection by iber optic microluidic biosensor
Zurina Zainal Abidin, Samla Gauri, Mohd Adzir Mahdi and Nurul Amziah Md Yunus
Universiti Putra Malaysia, Malaysia
P
athogens that usually associated with pathogenesis afect human health with massive diseases including chronic infections or
immune system disorders. Although numerous vaccinations have been introduced to protect against diseases, some pathogens
continues to threaten living life. he current pathogen detection method which based on molecular culture and PCR techniques
are essentially slow and time consuming. In recent time, several researchers have attempted to develop rapid detecting tool. Despite
advanced engineering, there is still need for an accurate and rapid pathogen detection tool. hus, this research was carried out to
highlight on detection of pathogens using optical biosensor. An optical biosensor (microchannel) is relatively an accurate method of
early detection of pathogen. Optical microchannel was fabricated with iber optics by using photolithography method. Fiber optic
biosensor is light scattering, absorption and optical properties of the microorganisms. he chemical composition, energy, the total
nucleotides and photo pigments will deine the absorption properties of each microorganism. E. coli was detected at region of 280
nm to 285 nm by using the microchannel while, S. cerevisiae identiied at visible region of 570 nm to 580 nm. he entire detection
can be done in less than 10 minutes with minimum required cells of 1×102 cells per mL with total volume of 6 µl. hese detection
regions for each sample were compared with spectrophotometer measurement plus theoretical calculations by using Beer Lambert
law of absorption.
Biography
Zurina Zainal Abidin has received her BEng in Chemical Engineering from Loughborough University, UK in 1997, Masters in Biotechnology at University of
Manchester Institute of Science and Technology, UK and PhD in 2006 from University of Manchester, UK in Biochemical Engineering. Earlier, she developed her
career as a Process Engineer in Elektrisola (M) Sdn Bhd in 1998. Later in 1999, she joined Universiti Putra Malaysia (UPM) and currently working as an Associate
Professor at Department of Chemical and Environmental Engineering. Her research areas focus on biochemical engineering application on bioseparation using
electrokinetics/microarray technology, extraction of bioproducts membrane iltration, biosensor and also wastewater treatment. She has successfully supervised
many postgraduate in similar disciplines and also published more than 30 articles in relation to biochemical engineering area.
zurina@upm.edu.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 65
Yiu Fai Tsang, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Bioiltration with Cyperus alternifolus for nutrient removal and water reuse in suburban areas
Yiu Fai Tsang
The Hong Kong Institute of Education, Hong Kong
B
ioilter, which capitalizes on the principle of constructed wetland in pollutant removal, reduce the concentration of pollutant
in wastewater passing through by a combination of physiochemical processes and biological processes. Apart from physical
adsorption, sedimentation and iltration occurred on the supporting media; bioilms are developed on the supporting media in
bioilter and are responsible for the nutrient removal processes. A bioilm forms when multilayers of bacteria, algae and fungi plus
microfauna embedded in a polymer matrix develop at a surface or as mobile bioilms or aggregates. Aggregates of microorganisms
grow at a solid-liquid interface, the support materials of bioilms are packed in the reactor in which the metabolic processes are
supposed to take place. Besides, plants in wetland system also contribute to the overall pollutant removal function, though the
proportion of pollutant removals (N & P) by plant uptake and harvesting was just around 10-15%. Vegetations in wetland system
serves to leak oxygen to the root zone, provide substrate for microbes, act as natural ilter for suspended solid, alter the hydraulic
retention time and take up nutrients such as nitrogen & phosphorus. he plant roots and the substrates in bioilter systems provide a
large surface area, which would certainly encourage the development of bioilm at the surface-water interface. By introducing plants
into bioilter system, on one hand can enhance pollutant removal eiciency and on the other hand can also reduce maintenance and
increases the life of the ilter. Sequencing batch technology, which commonly used in activated sludge treatment system, has proven
to be a viable alternative to continuous-low systems in carbon and nutrient removal from domestic and industrial wastewaters. he
wastewater is illed into and drawn away from the system alternatively and hence resulting “wet” and “dry” conditions in sequential
pattern. he intermittent pulse feeding sometimes is called as tidal low feeding, has been adopted in the vertical low bed system
to maintain adequate aeration and avoid clogging. he pollutant removal processes in the ilter bed are also diversiied in tidal low
reactor with regular changes of system environment. For instance, co-current nitriication and denitriication can be achieved within
the time frame of one cycle through simple adjustment of aeration density. Miller and Wolf (1975) have also shown that nutrient
adsorption capacity of vertical ilter bed can be regenerated if the system is allowed to rest and dry regularly during the operation.
In this study, a lab-scale vertical low cinder bed was set up to treat domestic wastewater. Cyperus alternifolius was planted into the
three of the six bioilter column beds operating with alternative changes of wet and dry periods. hree operating modes (tidal low
patterns) with diferent durations of wet and dry periods were investigated for their performances of pollutant removals, including
biochemical oxygen demand (BOD5), chemical oxygen demand (COD), ammonia (NH3-N), nitrate (NO3 -), total phosphorous
(TP) and total suspended solids (TSS).
Biography
Yiu Fai Tsang is currently an Assistant Professor in the Department of Science and Environmental Studies at the Hong Kong Institute of Education (HKIEd). He
has received his PhD from the Hong Kong Polytechnic University (PolyU). He has further worked as a Visiting Scholar in the Department of Agricultural and
Biological Engineering at the University of Illinois at Urbana-Champaign (UIUC). Prior to joining HKIEd, he was a Research Fellow in the Department of Civil and
Environmental Engineering at PolyU. In addition, he is the Program Leader of Master of Social Sciences in Community Education for Environmental Management.
tsangyf@ied.edu.hk
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 66
Yiu Fai Tsang et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Odour treatment in potable water using bioiltration with ozone addition
Yiu Fai Tsang1, M C Law2 and H Chua3
The Hong Kong Institute of Education, Hong Kong
2
The Hong Kong Polytechnic University, Hong Kong
3
Technological and Higher Education Institute of Hong Kong, Hong Kong
1
T
raditional water treatment processes cannot provide an efective removal of geosmin and MIB. he application of activated
carbon is one of the most commonly used treatment processes, however, the presence of natural organic materials can result
in competition for adsorption sites, leading to decreases in geosmin and MIB removal. Larger dose of activated carbon is required
for efective removal. A cost efective and practical method for the treatment of MIB and geosmin is therefore required. One of
the efective processes is ozone-enhanced bioiltration. Locating bioilters downstream of ozonation improves dissolved organic
carbon removal and can aid in producing biologically stable water such that the potential for bioilm re-growth in water distribution
systems is minimized. Field operational data suggests that ozone can oxide 10% to more than 90% of the Geosmin and MIB and
typical bioiltration can reach 50% removal only. Several factors may signiicantly inluence geosmin and MIB removal in bioilters,
including such as seasonal water temperature variations, ilter media (GAC, EC or sand), empty bed contact time. Some investigations
demonstrated that temperature and media are the most important factors afecting drinking water bioiltration processes and may
inluence the removal of compounds such as geosmin and MIB. In this study, the major factors afecting the biological degradation
of geosmin and MIB removal in bioilters, including initial concentration, empty bed contact time, ozone dosage and media were
examined.
Biography
Yiu Fai Tsang is currently an Assistant Professor in the Department of Science and Environmental Studies at the Hong Kong Institute of Education (HKIEd). He
has received his PhD from the Hong Kong Polytechnic University (PolyU). He has further worked as a Visiting Scholar in the Department of Agricultural and
Biological Engineering at the University of Illinois at Urbana-Champaign (UIUC). Prior to joining HKIEd, he was a Research Fellow in the Department of Civil and
Environmental Engineering at PolyU. In addition, he is the Program Leader of Master of Social Sciences in Community Education for Environmental Management.
tsangyf@ied.edu.hk
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 67
Syarul Nataqain Binti Baharum et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Metabolomics approach for biomarker discovery of Epinephelus fuscoguttatus infected with vibriosis
Syarul Nataqain Binti Baharum and Nurdalila Awani Abdul Aziz
Universiti Kebangsaan Malaysia, Malaysia
E
pinephelus fuscoguttatus or tiger grouper has excellent biological characteristics, fast growing and suitable for aquaculture. his
posed a promising industry but is unfortunately hindered by a host of diseases, which oten associated with feeding leading
to great economic loss. Our goal is to study the metabolite responses to vibrosis in grouper and identify unique metabolites as
biomarker to help future remediation/prevention of vibriosis. In this work, larvae of grouper were infected with vibriosis, under fed
and non-feed conditions. Metabolites were then extracted from infected (challenged) and non-infected (control) larvae, which died
and compared with that of survived. Metabolites proiled were obtained using GC-MS based metabolome analysis. he diferences
between the challenged and control samples were signiicantly inluenced by the production of fatty acids. A total of 11 fatty acids
under challenged feeding and 13 fatty acids under challenged starvation of ω-9 were detected. However, in the control feeding
and control starvation conditions, only four fatty acids of ω-9 were detected namely, lauric acid, myristic acid, palmitic acid and
trans-9-palmitoleic acid. he detected compounds were quantiied based on three diferent extraction techniques with the highest
at 34 mg/ml and the lowest at 17 mg/ml and mapped in the major fatty acids pathway. here were signiicant diferences (P≤0.001)
of ω-9 production between the challenged and control samples. hese changes demonstrate that vibriosis in the ish will induce a
biochemical response, especially ω-9 that can be used as biomarker to determine the survival rate of E. fuscoguttatus.
Biography
Syarul Nataqain Binti Baharum has received intensive training in the ield of Metabolomics at the University of Shefield, United Kingdom under supervision of
Professor Dr. Michael Burrell and Professor Dr. William Paul Quick. Her research is focused on the new insight of analytical and biological perspectives of the
metabolomics in the ield of systems biology. Her work has been awarded prestigious awards including BioInnovation Awards, 2011 and Selangor My Innovation
Award, 2014.
nataqain@ukm.edu.my
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 68
Hoda M Elzeini et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Morphological identiication of rod lactic acid bacteria using scanning electron microscope and image analysis
Hoda M Elzeini, A A Ali, N F Nasr and Ashwak A Hassan
Cairo University, Egypt
L
actic Acid Bacteria (LAB) widely distributed in nature and play an important role in much food fermentations. Bacterial cell
morphology is useful and valuable characteristics used in the identiication of any organism in order to classify. herefore, cell
morphological shape and size of four strains of rod LAB grown in two diferent media (MRS and RSM) were studied using Scanning
Electron Microscope (SEM) and image analysis technique. Topographical images reveal rectangular shapes with smooth corners and
height proiles and variation in size which occurred singly or in pairs or chains. Rod cells had a wide range of breadth (0.40-1.07 μm)
with cells' perimeters of 4.64 up to 11.7 μm and the elongation varies between 0.572 and 0.790 µm. Both areas of Lb. helveticus 764N
and Lb. casei 761N were reduced when grown in RSM than in broth medium. On the contrary, cells' areas of Lb. acidophilus 791N and
Lb. paracasei 72MP increased when grown in RSM than in broth medium. RSM lowered the compression on cells walls either by the
adsorbed water to the surface of the cells or by extra nutrients provides. All the aseptic ratios measured were higher than 1. Rod cells
had a diferent orientation angles and surfaces seem to have heights or extrudes with external boundary was either a sharp outline or
a rough surface. Cells hardness and wall thickness afected negatively or positively depending on the strain and growing medium. In
conclusion, image analysis of SEM could be a good and accurate tool for measuring many morphology, shape and size parameters for
the viable bacterial cells which can be used for identiication and classiication of rod LAB with determination of deined standard
conditions for optimum growth.
Biography
Hoda M Elzeini has completed her PhD from Michigan State University, USA. She is a Professor in Cairo University, Dairy Science Department of Faculty of
Agriculture in Egypt. She has published more than 20 papers in reputed journals and has been serving as a Reviewer for some journals. She is teaching dairy
technology and food rheology courses. She was trained in England and India to use advance rheological apparatuses.
dr_hodazeini@yahoo.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 69
Xiaoying Helen Lu et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Exploring active microbial community of anaerobic digestion systems by 454 pyrosequencing
Xiaoying Helen Lu, K L Pun and H Chua
Technological and Higher Education Institute of Hong Kong, Hong Kong
B
iotransforming organic waste into energy has been regarded as one of the promising technologies to combat energy crisis in the
future. In this study, we queried the highly active functional population in the anaerobic digestion system with various potential
substrates (food waste, cellulose, xylan) by integrating the sludge DNA and RNA of subculture from the substrates. Using barcoded
454-pyrosequencing, we analyzed and compared the bacterial and archaeal 16S rRNA genes of one digester sludge (DNA) sample
and three subcultures (cDNA) by giving three individual substrates to the digester sludge in order to identify the active members in
digesting organic wastes to biomethane. A total of 19K bacterial and 13K archaeal efective sequences were assigned to taxonomy
from phylum to genera levels, providing insights into the microbial community structure and further correlating the community
members to the performance of the subculture. he principal coordinates’ analyses on the basis of UniFrac of OTUs revealed that
there are signiicant diferences among the digester sludge and digestion culture samples. Cellulose and xylan culture community are
more closer to each compared to food waste culture, even though it still has a slight diference between cellulose and xylan culture.
Overall, the phylogenetic information based on DNA and RNA provides a comprehensive and systematic view of the anaerobic
microbial community within the digestion systems.
Biography
Lu Xiao Ying has obtained his PhD in Civil Engineering from the University of Hong Kong, Hong Kong. She is currently an Assistant Professor of the Faculty
of Science and Technology, Technological and Higher Education Institute of Hong Kong (THEi). She is specialized in environmental microbiology, bioenergy
production from solid waste and advanced materials development in energy and environment.
xylu@vtc.edu.hk
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 70
Dora Garcia Delgado, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Methodology for the valuation and negotiation of biotechnology products with intangible assets
Dora Garcia Delgado
Center for Genetic Engineering and Biotechnology of Cuba, Cuba
H
eber Biotec S A, a Commercialized Company from the Center of Genetic Engineering and Biotechnology of Cuba. he
High Tech Enterprise is playing an important role in the economy among other aspects as a source of high aggregated value
products and intangible assets. he biotechnology enterprise is an exponent of this enterprise. Upon the basis of a document
analysis and experts interchange applying Delphis method, a diagnostic is done concerning the treatment given to the biotechnology
products with intangibles during negotiations. From such diagnostic the lack of a standard calculation method and the lack of a
guide for preparing negotiations were identiied as well as the lack of commercial culture concerning the biotechnology products
with intangibles negotiation. he general objective of this work is to design a methodology for the valuation and deal making of
biotechnology products. For the fulillment of this objective a deep bibliography was reviewed granting the required concepts to
design the methodology which establishes a sequence for the negotiation, recommends a calculation method and some accurate data
like the range of payments, royalties percentages, coeicients to separate the intangibles form the total value of the biotechnology
project or product. In the whole bibliography reviewed no other guide was found with the integration of calculation method and
negotiation methodology in a sequence that become this methodology in a practical tool that allow to entrepreneur who start in
biotechnology to evaluate and prepare a biotechnology product negotiation.
Biography
Dora Garcia Delgado is graduated in Foreign Trade Economy, Diplomat in Foreign Trade and Masters in Science and PhD in Economic Science from Havana
University. She was trained at Business Support Centers Japan 2001 and Seoul 2003. She has participated in several negotiations of Cuban biotechnology
products. She has publications in Journal Applied Biotechnology of Cuba: About negotiation of Biotechnology products with intangibles, the evolution of negotiation
of biotechnology products further than the intellectual property protection and methodology for the valuation and negotiation of biotechnology products with
intangibles.
dora.garcia@heber-biotec.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 71
Mahima Gulabani, J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Age at natural menopause and osteoporosis: A genomic appraisal
Mahima Gulabani
University of Delhi, India
M
enopause (end of menstruation) is a hallmark event concerned with the end of female’s reproductive life. hroughout the
last decade the interest in the mechanisms behind ovarian aging and the timing of natural menopause has increased since
it has a great cultural, social and epidemiological implications for female’s fertility, health and health risks. Osteoporosis, one of
the profoundly postmenopausal associated health risk, as stated by WHO (1991) is “ a disease characterized by low bone mass
and micro-architectural deterioration of bone tissue, leading to enhanced bone fragility and a consequent increase in fracture risk”.
Studies have revealed menopause causes loss of ovarian steroids and estrogen that have been signiicant correlated with loss of bone
mineral density. Also, genomic markers particularly concerned with the variants of Vitamin D receptor gene, ATP6V1G, ESR1,
MHC, COLIA 1 and TGF-β1 genes have been found to be associated with decreased bone density and therefore serves to be a
potential marker for estimating osteoporosis risk. hus, an attempt to evaluate this menopause associated genes in addition with an
insight into the lifestyle factors must be done in diferent populations, contributing to the development of holistic women speciic
public health related policies.
Biography
Mahima Gulabani is a graduate and post-graduate in Anthropology with specialization in Biological Anthropology, with an excellent academic record from the
University of Delhi. I am currently a Senior Research Fellow at University of Delhi, pursuing my Ph.D. in Molecular Anthropology (Molecular Genetics) with regard
to study related to women’s reproductive health among Indian Populations.
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 72
Surya Anjani Kumar et al., J Biotechnol Biomater 2016, 6:3 (Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.055
conferenceseries.com
10
Asia-Paciic Biotech Congress
th
July 25-27, 2016 Bangkok, Thailand
Night soil a potent plant growth enhancer
Surya Anjani Kumar and Archana Giri
Jawaharlal Nehru Technological University, India
B
lack soot is the other name for Night soil. Night soil is the euphemism of composted feces. Night soil is generally collected by
sanitation department by tankers and disposed at the outskirts of the towns or cities. he composted humanure is a very rich
source of micro and macro nutrients which are very useful for plants. he idea behind the work is to utilize this black soot as a
biofertilizer for enhancing the growth of plants. It is a process of bioremediation where the compost is produced in situ. he compost
is generally disposed but if this compost is air dried and added by some microbial mixtures it is an excellent biofertilizer. Current
work majorly concentrates on microbial mixtures which are to be added to the black soot to use it for potted plants, Gardens, Bonsai
& ields. he collected black soot alone is tested for NPK values and carbon content. To the surprise it is found to be very high than
normal values making it suitable for using it as a potential organic fertilizer. Pure cultures of Nostoc, Azolla, Anabena, Trichoderma,
VAM, PSB etc are maintained and are added to the black soot in diferent proportions to check for the better combination and good
results. To the surprise the process opened new avenues for research in formulating an excellent biofertilizer suitable for all varieties
of plants, enhancing their growth, fruiting, yield & resistance.
Biography
Surya Anjani Kumar is currently pursuing Post doctorate at JNTU Hyderabad working on “Effect of Fresh Water Crab Shell Fog on Cucurbitaceae” and identifying
biological growth promoters. His work is mainly concentrated on applying fog at ield level, observing phenotypic changes & identifying the novel volatile compound
which is responsible for the effect speciically on Cucurbitaceae.
s.anjanikumar@gmail.com
Notes:
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio Asia-Paciic 2016
Volume 6, Issue 3 (Suppl)
July 25-27, 2016
Page 73