Systematic Parasitology 49: 89–112, 2001.
© 2001 Kluwer Academic Publishers. Printed in the Netherlands.
89
Biogeography and phylogeny of Dermoergasilus Ho & Do, 1982
(Copepoda: Ergasilidae), with descriptions of three new species
H. H. El-Rashidy1,2 & G. A. Boxshall1
1 Department
2 Queen
of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD, UK
Mary and Westfield College, University of London, Mile End Road, London E1 4NS, UK
Accepted for publication 18th August, 2000
Abstract
Three new species of Dermoergasilus are described from six species of grey mullet hosts. Dermoergasilus longiabdominalis n. sp. was found on Valamugil engeli (Bleeker) from the Philippines and Madagascar and on V. cunnesius
(Valenciennes) from the Philippines and Mangalore, India. D. semiamplectens n. sp. occurred on Sicamugil hamiltoni (Day) from the Sittang River, Burma, on Liza subviridis (Valenciennes) and L. parsia (Hamilton Buchanan)
from Calcutta, India, and on V. cunnesius (Valenciennes) from China. D. curtus n. sp. parasitised Rhinomugil
squamipinnis (Swainson) from Alahabad, India. A key to the ten currently accepted species of Dermoergasilus
is given. The biogeographical distribution of Dermoergasilus species is analysed and levels of host-specificity
are surveyed within the genus. The phylogenetic relationships between the species of Dermoergasilus are also
analysed.
Introduction
The genus Dermoergasilus was proposed by Ho & Do
in 1982 to accommodate Ergasilus amplectens Dogiel
& Akhmerov, 1952 (reported from Mugil cephalus
L. from Russia and Japan), Dermoergasilus coleus
(Cressey, 1970) (on needlefishes from the Philippines, Borneo and India) and D. semicoleus (Cressey,
1970) (on needlefishes from Australia). Subsequently,
Byrnes (1986) described a new species, D. acanthopagri, from three species of bream from Australia and a
fifth species, D. mugilis, was added by Oldewage &
van As (1988) from Mugil cephalus in South Africa.
Kabata (1992) described a species of Ergasilus Nordmann, E. intermedius, from several hosts belonging to
the families Clupeidae, Plotosidae and Perichthyidae.
He named this species to reflect its intermediate nature
between the genera Ergasilus and Dermoergasilus.
This species was later transferred to Dermoergasilus
by El-Rashidy & Boxshall (1999). Ho et al. (1992) reported D. amplectens on Valamugil seheli and on five
other hosts belonging to the families Cichlidae, Gerreidae, Hemiramphidae, Megalopidae and Chanidae
from India, in addition to describing a new species,
D. varicoleus Ho, Jayarajan & Radhakrishnan, 1992,
found on Liza tade (Forsskål). This new species was
subsequently reported on a second mugilid host, Liza
abu (Heckel), from Iraq (Ho et al., 1996).
The present paper describes three new Dermoergasilus species, all of which occur on grey mullet
(Mugilidae). Additional new localities and new host
records are presented for D. amplectens and D. varicoleus, together with a key to the ten currently accepted Dermoergasilus species. This genus is confined
to the Indian and Indo-West Pacific regions. The phylogenetic relationships of species within the genus
Dermoergasilus were analysed using PAUP. We consider that the status of the genus Dermoergasilus is
still uncertain. However, validation of the genus can
only be achieved in the context of a full generic level
revision of the entire family Ergasilidae, given that
the most speciose genus, Ergasilus, is certainly paraphyletic (El-Rashidy, 1999). No changes will be
proposed here.
Materials and methods
Material was collected from the gills of grey mullet
deposited in the fish collection of The Natural His-
90
tory Museum, London (BMNH). The copepods were
removed from the gill filaments, dissected, mounted
in lactophenol as temporary slide preparations. Measurements were made using an ocular micrometer
and drawings were made with the aid of camera lucida on an Olympus BH2 microscope equipped with
differential interference contrast.
Dermoergasilus longiabdominalis n. sp.
(Figures 1–4)
Type-material: Female holotype (BMNH Reg. No.
1999.1318), 11 female paratypes (BMNH Reg. Nos
1999. 1319-1329).
Type-locality: Calabato, Mindanao, Philippines.
Type-host: Valamugil engeli (Bleeker).
Records of infected hosts: V. engeli (Mindanao, Philippines; Tamatave, Madagascar), V. cunnesius (Valenciennes) (Mindanao, Philippines; Mangalore, India).
Etymology: The specific name refers to the elongate
urosome.
Description of female
Mean body length 0.89 ± 0.08 mm, mean body width
0.24 ± 0.01 mm (n = 3). Cephalothorax constituting
about half body length (excluding caudal setae); anterior part narrow, delimited by posterior margin of
dorsal cephalic shield, ornamented with inverted Tshaped marking (Figure 1A). First pedigerous somite
partly delimited by dorsal constriction visible in lateral
view (Figure 1B) and with median longitudinal fold
dorsally. Free second to fourth pedigerous somites narrowing posteriorly. Rostrum small, rectangular. Thick
block of muscles extending from dorsal origin on
mid-line to base of antennae (Figure 1C).
Genital double-somite elongate, 1.2-1.5 times
longer than first abdominal somite; anterior part wider
than posterior; rows of spinules present ventrally in
anterior part (Figure 1D). First free abdominal somite
1.5-1.7 times longer than wide, and nearly 1.5 times
longer than second and third abdominal somites and
caudal rami combined, ornamented with row of spinules ventrally in anterior part. Second free abdominal somite nearly as long as anal somite and caudal
rami combined, with row of spinules ventrally along
posterior margin. Anal somite deeply incised medially; ornamented with posterior spinule row. Caudal rami nearly 2/3 length of anal somite, produced
disto-ventrally into narrow digitiform process bearing
minute terminal seta; process 2.4-2.8 times longer than
ramus; longest caudal seta armed with rows of spinules; 2 lateral setae longer than digitiform process;
longer lateral seta sparsely plumose.
Antennule small, 6-segmented (Figure 2A), tapering distally; setal formula: 3: 10: 5: 4+ae: 2+ae:
7+ae.
Antenna 4-segmented (Figure 2B), comprising
short coxobasis and 3-segmented endopod bearing
curved claw. Base of antenna elevated, carried on
strongly developed pedestal (arrowed in Figure 2B)
nearly half as long as coxobasis. Second segment (first
endopodal segment) nearly twice as long as coxobasis.
Second endopodal segment long, armed with small
spine nearly at mid-length of concave margin. Third
endopodal segment very small, represented by incomplete sclerite. Second plus third endopodal segments
together comprising nearly 85% length of first endopodal segment. Curved claw nearly 2/3 length of
second endopodal segment, fitting into distal socket,
located in inflated cuticular membrane surrounding
first endopodal segment of opposing antenna.
Mandible unsegmented, bearing anterior, distal
and posterior blades; small anterior blade with teeth
on anterior margin; distal blade with teeth along both
margins; posterior one with teeth on posterior margin. Maxillule lobate with medial process plus 2 distal setae and minute inner seta. Maxilla consisting
of large syncoxa tapering distally and small spatulashaped basis, armed anteriorly with rows of sharp teeth
(Figure 3A).
Legs 1-4 (Figures 3B; 4A-B) with all rami 3segmented except 2-segmented fourth exopod. Basis
with row of spinules along posterior margin in legs 24. Lateral margins of both rami spinulate in all legs.
Setules present on unarmed inner margin of first exopodal segment of all legs. Interpodal sternites not
ornamented (Figure 4C). Armature of legs as follows:
Leg 1
Leg 2
Leg 3
Leg 4
Coxa
0-0
0-0
0-0
0-0
Basis
1-0
1-0
1-0
1-0
Exopod
I-0; 0-1; II,5
I-0; 0-1; 6
I-0; 0-1; 6
I-0; 5
Endopod
0-1; 0-1; II,4
0-1; 0-1; I,4
0-1; 0-1; I,4
0-1; 0-2; I,3
Fifth leg 2-segmented; short protopodal segment
bearing outer seta; free exopodal segment with short
lateral seta plus 2 terminal setae (Figure 3C).
91
Figure 1. Dermoergasilus longiabdominalis n. sp. Adult female. A, dorsal view; B, lateral view; C, rostrum, ventral; D, urosome, ventral.
Scale-bars in µm.
92
Figure 2. Dermoergasilus longiabdominalis n. sp. Adult female. A, antennule; B, antenna, with pedestal arrowed. Scale-bars in µm.
93
Figure 3. Dermoergasilus longiabdominalis n. sp. Adult female. A, mouthparts; B, first swimming leg, anterior; C, fifth swimming leg.
Scale-bars in µm. (mn = mandible, ml = maxillule, mx = maxilla).
94
Figure 4. Dermoergasilus longiabdominalis n. sp. Adult female. A, second swimming leg; anterior; B, fourth swimming leg, anterior; C,
interpodal sternite of second swimming leg. Scale-bars in µm.
Remarks
The new species shares an elongate urosome with D.
mugilis, D. acanthopagri, D. amplectens and D. varicoleus. The South African species, D. mugilis, differs
from the new species in the setation of the antennule
(but this needs verification) as well as in the setal
formula of the swimming legs. The new species differs from D. acanthopagri in the antennulary setation
and in the presence of five setae on the terminal exopodal segment of leg 4, compared with six in D.
acanthopagri.
The new species resembles D. amplectens in the
setal formula of the swimming legs and in the ornamentation of the first exopodal segment of legs 1-4.
It differs from D. amplectens in the antennulary setation and in having a first free abdominal somite that
is longer than wide (that of D. amplectens is only
nearly as long as wide). The coxobasis of the antenna
is carried on an elevated anterolateral pedestal in both
species, but it is smaller in the new species than in
D. amplectens. The caudal digitiform process of the
new species is long and narrow, whereas that of D.
amplectens is relatively short and wide. A small terminal seta is present on the digitiform process in both
species but has not previously been reported in D. amplectens (it was overlooked in the original description,
see below).
The new species is closely related to D. varicoleus
with which it shares the leg setation formula and
elongate urosome, but it differs particularly in the proportional lengths of the antennal segments. The tip of
the digitiform process on the caudal ramus is rounded
and bears a small seta. It is similar to that of D. varicoleus (which is present although not mentioned in the
original description). The lateral caudal setae in the
new species are longer than the digitiform process, but
are shorter than it in D. varicoleus.
95
Dermoergasilus semiamplectens n. sp. (Figures 5-8)
Type-material: Female holotype (BMNH Reg. No.
1999.1340), 38 female paratypes (BMNH Reg. Nos.
1999.1341-1378).
Type-locality: Sittang River, Burma.
Type-host: Sicamugil hamiltoni (Day).
Records of infected hosts: S. hamiltoni (Sittang River,
Burma), Valamugil cunnesius (China), Liza subviridis (Calcutta, India) and Liza parsia (Hamilton
Buchanan) (Calcutta).
Etymology: The specific name refers to the close
resemblance between the new species and D. amplectens.
Description of female
Mean body length 0.70 ± 0.04 mm; range 0.65–
0.77 mm. Mean body width 0.23 ± 0.01 mm; range
0.21–0.26 mm (n = 10). Cephalothorax oblong,
slightly constricted in posterior third; dorsal surface
ornamented anteriorly with inverted T-shaped marking (Figures 5A, B). Boundary between cephalosome
and first pedigerous somite indistinct. Free second
to fourth pedigerous somites narrowing posteriorly.
Rostrum with 6 sensilla and 3 integumental pores
(Figure 5C).
Genital double-somite slightly longer than 3 free
abdominal somites combined; anterior part wider than
posterior; ventral spinule row present at level of genital openings (Figure 5D). First free abdominal somite
constituting about 55–66% length of genital doublesomite, just wider than long, and slightly longer than
second and third free abdominal somites plus caudal
rami combined; transverse row of spinules present
ventrally in anterior third of somite. Second free abdominal somite slightly longer than anal somite, ornamented with transverse row of spinules ventrally. Anal
somite deeply incised medially and ornamented ventrally with incomplete row of spinules along posterior
margin. Caudal rami (Figure 5D) about equal in length
to anal somite; each produced distoventrally into stout
digitiform process, about 1.8 times longer than ramus, bearing short seta; longest seta ornamented with
spinule rows; 2 lateral setae longer than digitiform
process.
Antennule small, 6-segmented (Figure 6A), tapering distally; setal formula: 3: 10: 5: 4+ae: 2+ae:
7+ae.
Antenna 4-segmented (Figure 6B), comprising
coxobasis, 3 endopodal segments and curved claw.
Base of antenna elevated on weak pedestal. Second
segment (first endopodal segment) nearly 2.2 times
longer than coxobasis (excluding pedestal elevating
coxobasis); second and third endopodal segments together equal to 60% length of first endopodal segment.
Curved claw about 2/3 length of second plus third endopodal segments combined; tip fitting into distal part
of first endopodal segment of opposing antenna. Antennal segments enclosed by loose, hyaline, cuticular
membrane.
Mandible unsegmented, bearing 3 blades; anterior
blade with teeth on anterior margin; large distal blade
carrying teeth on both margins; posterior blade with
teeth only on posterior margin. Maxillule lobate, ornamented with spinules, bearing 2 long distal setae,
minute inner seta and tapering medial process. Maxilla
consisting of large tapering syncoxa and small spatulashaped basis, armed anteriorly with irregular rows of
sharp teeth (Figure 7A).
Legs 1-4 (Figures 7B, 8A-B) with all rami 3segmented except 2-segmented fourth exopod. Basis
with row of spinules along inner margin in legs 24. Lateral margins of both rami spinulate in all legs.
Setules present on inner margin of first exopodal segment of all legs. Interpodal sternites spinulate (Figure 7B). Spine and setal formula of legs 1-4 as in D.
longiabdominalis n. sp.
Fifth leg 2-segmented; protopodal segment short
with outer seta; free exopod with short lateral seta plus
2 setae of unequal length at apex (Figure 7C).
Remarks
The new species is closely related to D. amplectens.
It possesses the same swimming leg setation, the first
abdominal somite is about as long as wide, the base of
the digitiform process on caudal ramus is broad, and
the process itself is short. It differs from D. amplectens
in its antennulary setation and in the reduction of
the anterolateral cephalic pedestal bearing the antenna
which causes the antenna to appear to be 5-segmented
in D. amplectens.
The new species differs from D. varicoleus in the
proportional lengths of the first free abdominal somite
and genital double-somite: it represents up to twothirds of the genital double-somite length in the new
species but about three-quarters in D. varicoleus. The
first abdominal somite is nearly as long as wide in the
new species, whereas it is 1.7 times longer than wide
in D. varicoleus. Finally, the digitiform process on the
caudal ramus is relatively longer in D. varicoleus.
96
Figure 5. Dermoergasilus semiamplectens n. sp. Adult female. A, dorsal view; B, inverted T-shaped marking; C, rostrum, ventral; D, urosome,
ventral. Scale-bars in µm.
97
Figure 6. Dermoergasilus semiamplectens n. sp. Adult female. A, antennule; B, antenna. Scale-bars in µm.
98
Figure 7. Dermoergasilus semiamplectens n. sp. Adult female. A, mouthparts; B, first swimming leg and interpodal sternite, anterior; C, fifth
swimming leg. Scale-bars in µm.
99
Figure 8. Dermoergasilus semiamplectens n. sp. Adult female. A, second swimming leg; anterior; B, fourth swimming leg, anterior. Scale-bars
in µm.
Dermoergasilus curtus n. sp. (Figures 9-12)
Type-material: Female holotype (BMNH Reg. No.
1999.1628).
Type-locality: Alahabad, India.
Type-host: Rhinomugil squamipinnis (Swainson).
Etymology: The specific name refers to the short
urosome of the female.
Description of holotype female
Body length 0.76 mm, body width 0.24 mm.
Cephalothorax oblong, ornamented with inverted Tshaped marking anteriorly (Figure 9A). Free second
to fourth pedigerous somites narrowing posteriorly.
Genital double-somite globular, wider than long,
nearly 2.2 times longer than first free abdominal
somite, and nearly as long as second and anal somites
combined; ornamented with 3 transverse rows of spin-
ules ventrally (Figure 10A). Folded membrane present
between genital double-somite and following somite.
First free abdominal somite nearly twice as wide
as long; ventral spinule row present along posterior
margin; folded membrane present between first and
second free abdominal somites. Second somite nearly
twice as wide as long, with ventral row of spinules
along posterior margin. Anal somite 1.5 times wider
than long, incised medially; ventral row of spinules
present along posterior margin. Caudal rami produced
distally into digitiform process bearing minute terminal seta; process nearly twice as long as ramus; long
medial seta on posterior margin armed with spinule
rows; 2 lateral setae shorter than digitiform process.
Antennule small, 6-segmented (Figure 10B), tapering distally; setal formula: 3: 10: 5: 4+ae : 2+ae:
7+ae.
100
Figure 9. Dermoergasilus curtus n. sp. Adult female. A, dorsal view; B, antenna. Scale-bars in µm.
101
Figure 10. Dermoergasilus curtus n. sp. Adult female. A, urosome; B, antennule. Scale-bars in µm.
102
Antenna 4-segmented (Figure 9B), comprising
coxobasis, 3-segmented endopod and curved claw.
Coxobasis on slightly elevated pedestal. Second segment (first endopodal segment) nearly 1.8 times longer
than coxobasis; long second plus third endopodal segments together comprising about 70% of length of
first endopodal segment; curved claw about 3/4 length
of second plus third endopodal segments combined;
antenna with slightly inflated cuticle around first endopodal segment only; second endopodal segment
with minute seta proximally on inner margin.
Mandible unsegmented, bearing anterior, distal
and posterior blades; teeth present on anterior margin
of anterior blade, both margins of distal blade and on
posterior margin of posterior blade. Maxillule lobate,
ornamented with spinules, bearing 2 long distal setae,
minute inner seta and small medial process. Maxilla
consisting of large syncoxa tapering distally and small,
spatula-shaped basis armed anteriorly with rows of
sharp teeth (Figure 11A).
Legs 1-4 (Figures 11B-C, 12A-B) with all rami
3-segmented except 2-segmented fourth exopod. Basis with row of spinules along posterior margin in
legs 2-4. Lateral margins of both rami spinulate in
all legs. Setules present on inner margin of first exopodal segment of all legs. Interpodal sternites spinulate (Figure 12C). Setal formula of legs as in D.
longiabdominalis n. sp.
Fifth leg 2-segmented; short protopodal segment
bearing outer seta; free exopodal segment with one
short lateral seta plus 2 terminal setae (Figure 12D).
Remarks
This species differs from all other Dermoergasilus
species, except for D. intermedius and D. semicoleus,
in the presence of the hyaline inflated cuticle only
around the first endopodal segment of the antenna, and
by the small urosome with its short barrel-shaped genital double-somite and first free abdominal somite. The
presence of zones of folded membrane between the
genital double-somite and first free abdominal somite,
and the first and second free abdominal somites, might
be interpreted as evidence that the urosomal configuration of this species is somewhat intermediate between
Ergasilus and typical Dermoergasilus.
The new species differs from D. semicoleus in the
armature of the terminal endopodal segment of leg 1,
in carrying two outer spines and four inner setae instead of two spines and five setae, respectively. The
terminal exopodal segment of leg 2 is armed with six
setae in D. curtus n. sp. compared to five in D. semicoleus, and the terminal endopodal segment of leg 2
carries a spine and four inner setae rather than a spine
and three inner setae as in D. semicoleus.
The new species shares with D. intermedius the setation of the swimming legs; but it is relatively smaller
(D. intermedius is 0.9-1.04 mm) and the anterior margin of the cephalothorax is different (in D. intermedius
it is flat and the antennules are widely separated).
Additional differences include the shape of the first endopodal segment of the antenna (which is more curved
than that of D. intermedius), and the lateral seta on
leg 5 is relatively longer than the knob-like lateral seta
of D. intermedius; furthermore, the folded membranes
between the genital double-somite and the first free
abdominal somite, and between first and second free
abdominal somites, are absent in D. intermedius. The
lateral caudal setae are also both shorter than the digitiform process in D. curtus, whereas they are of unequal
lengths, with the outer being longer than the digitiform
process, in D. intermedius.
Dermoergasilus amplectens (Dogiel & Akhmerov,
1952) (Figures 13-14)
Previous records: This species was originally recorded
from the mouth of River Tumen-Ula in Russia, on
Mugil cephalus L. (see Dogiel & Akhmerov, 1952). It
has subsequently been recorded on the same host from
Kojima Bay, Okayama, Japan, by Ho & Do (1982).
It has also been reported from many localities around
Australia (Kabata, 1992) and India (Ho et al., 1992).
Present records: Wakanoura and Tsu Shima, Japan;
Kowie River, South Africa; all on Mugil cephalus.
Remarks
The specimens from Wakanoura are relatively smaller
(0.91±0.04 mm long, 0.30±0.02 mm wide) than those
from Tsu Shima (1.2 ± 0.2 mm long, 0.31 ± 0.01 mm
wide). The markings on the cephalothorax are the
same as in other Dermoergasilus species (Figure 13A).
The genital double-somite and the first abdominal
somite have a sclerotised cuticle and the two posterior abdominal somites are always reflexed anteriorly
in fixed specimens (Figure 13B). The caudal rami are
wide and the digitiform process is nearly twice as long
as the ramus (Figure 13C). The material differs from
previous descriptions in the presence of a minute terminal seta on the digitiform process, but this seta was
probably overlooked by previous authors.
103
Figure 11. Dermoergasilus curtus n. sp. Adult female. A, mouthparts; B, first swimming leg, anterior; C, exopod of first swimming leg,
posterior. Scale-bars in µm.
104
Figure 12. Dermoergasilus curtus n. sp. Adult female. A, second swimming leg, anterior; B, fourth swimming leg, anterior; C, interpodal
sternite of the second swimming leg; D, fifth swimming leg. Scale-bars in µm.
The swimming leg setation is the same as in D.
longiabdominalis n. sp. and leg 1 is characterised
(Figure 13D) by the relative lengths of the outer margin spines in the exopod and the distal spines on the
endopod.
The present specimens have a 6-segmented antennule, with setation as described by previous authors,
and the antenna is completely ensheathed with inflated
cuticle except for the claw. The coxobasis is elevated
on a long anterolateral pedestal, and both anterior and
lateral blocks of muscle are visible (Figure 14A, B).
The material from Kowie River, South Africa,
has a mean length of 0.94 ± 0.04 mm and mean
width of 0.36 ± 0.01 mm. This material closely resembles the Japanese material, but the anterolateral
pedestal bearing the antenna is less well developed
(Figure 14C).
Dermoergasilus varicoleus Ho, Jayarajan &
Radhakrishnan, 1992 (Figure 15)
Previous records: This species was originally recorded
on Liza tade (Forsskål) from Veli Lake, Trivandrum,
India (Ho et al., 1992) and subsequently from Liza abu
(Heckel) in Iraq (Ho et al., 1996).
Present records: Liza subviridis (Val.) (Calcutta,
Orissa, Madras and Bombay).
Material
The paratype material (USNM Reg. No. 254502) was
re-examined. It was confirmed that the second endopodal segment of the antenna comprises about 60% of the
length of the first endopodal segment (Figure 15A) and
that the first free abdominal somite constitutes nearly
three-quarters the length of the genital double-somite.
The digitiform process on the caudal ramus bears a
minute terminal seta (Figure 15B).
105
Figure 13. Dermoergasilus amplectens. Adult female. A, dorsal view; B, urosome; C, caudal ramus and digitiform process, lateral; D, first
swimming leg, anterior. Scale-bars in µm.
106
Figure 14. Dermoergasilus amplectens. Adult female. A, antenna, dorsal; B, antenna, lateral; C, antenna, ventral. Scale-bars in µm.
107
Figure 15. Dermoergasilus varicoleus. Adult female. A, antenna; B, anal somite and caudal rami. Scale bars in µm.
Key to the species of Dermoergasilus
1 Genital double-somite elongate (more than 1.3
times longer than wide) . . . . . . . . . . . . . . . . . . . . . . 2
– Genital-double somite short or ensheathed with
membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 Long anterolateral pedestal elevating base of
antenna (Figure 14A-B); loose, cuticular membrane completely ensheathing antennal segments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . amplectens
108
Figure 16. The reported geographical distributions of Dermoergasilus species.
– Short pedestal elevating base of antenna; cuticular membrane not completely ensheathing antennal
segments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
3 Second exopodal segment of leg 4 with 6
setae . . . . . . . . . . . . . . . . . . . . . . . . . . . . acanthopagri
– Second exopodal segment of leg 4 with 5
setae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
4 First endopodal segment of legs 1-4 without inner
seta. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . mugilis
– First endopodal segment of legs 1-4 with inner
seta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
5 First abdominal somite about as long as wide;
digitiform process on caudal ramus short (Figure 5D) . . . . . . . . . . . . . . . . . . . . . . . semiamplectens
– First abdominal somite longer than wide;
digitiform process on caudal ramus long (Figure 1D) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
6 Second plus small third endopodal segments of antenna long, curved, about 75% of first endopodal
segment . . . . . . . . . . . . . . . . . . . . . longiabdominalis
– Second plus small third endopodal segments of
antenna short, about 60% of first endopodal
segment . . . . . . . . . . . . . . . . . . . . . . . . . . . . varicoleus
7 Genital double-somite ensheathed by cuticular
membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . coleus
– Genital double-somite not ensheathed by cuticular
membrane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
8 Terminal endopodal segment of leg 1 with 5 setae;
leg 2 with 5 setae on terminal exopodal segment
and 3 on terminal endopodal segment . semicoleus
– Terminal endopodal segment of leg 1 with 4
setae; leg 2 with 6 setae on terminal exopodal segment and 4 on terminal endopodal
segment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
9 Antennules widely separated; first endopodal segment of antenna fairly straight; cuticular membrane not inflated, but loosely ensheathing all
antennal segments; lateral seta on leg 5 knoblike; folded membrane not present between
urosomites . . . . . . . . . . . . . . . . . . . . . . . . intermedius
– Antennules not widely separated; first endopodal segment of antenna slightly curved; cuticular
109
Figure 17. A. Single cladogram of Dermoergasilus species obtained from phylogenetic analysis using PAUP 3.1.1, Branch and Bound search.
B. Host family utilisation of Dermoergasilus species.
membrane limited, developed only on inner surface of first endopodal segment of antenna; lateral
seta on leg 5 not knob-like; folded membrane
present between urosomites . . . . . . . . . . . . . . curtus
Distribution of Dermoergasilus species and host
utilisation
Five species of Dermoergasilus were recorded on
hosts from the Indo-West Pacific region (Figure 16),
three which are new. D. longiabdominalis n. sp. was
found on three species of Valamugil: V. engeli from
the Philippines and Madagascar; V. cunnesius from the
Philippines and India (Figure 16); and V. seheli from
Sri Lanka. D. semiamplectens n. sp. was recorded on
four host species belonging to three genera of grey
mullet: Sicamugil hamiltoni from Burma; V. cunnesius from China; and Liza subviridis and Liza parsia
from India. D. curtus n. sp. was found on Rhinomugil
squamipinnis from India.
D. amplectens was found on Mugil cephalus from
Japan and S. Africa, and D. varicoleus recorded on L.
110
Table 1. Character matrix for phylogenetic analysis of Dermoergasilus species.
Character number
Taxon name
1
2
3
4
5
6
7
8
9
10
11
12
D. amplectans
D. varicoleus
D. acanthopagri
D. coleus
D. semicoleus
D. mugilis
D. intermedius
D. longiabdominalis
D. semiamplectans
D. curtus
Ergasilus sieboldi
1
1
1
?
0
1
0
1
1
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
0
1
0
1
0
0
0
0
0
0
1
1
?
0
1
0
1
0
0
0
2
2
2
2
1
2
1
2
2
0
0
1
1
1
1
1
1
1
1
1
1
0
0
1
0
1
1
0
0
1
0
0
?
Table 2. Characters and character states of Dermoergasilus species used in phylogenetic analysis (using
PAUP 3.1.1).
No
Character
States
0 = not elongate, 1 = elongate
0 = present, 1 = absent
0 = present, 1 = absent
0 = present, 1 = absent
0 = present, 1 = absent
0 = present (5 setae), 1 = absent (4 setae)
7
8
9
10
Genital double-somite shape
Inner seta on endopod segment 1, leg 1
Outer spine on exopod segment 1, leg 2
Inner seta on endopod segment 1, leg 2
Outer spine on exopod segment 1, leg 3
Inner proximal seta on exopod segment 3
of leg 3
Inner seta on endopod segment 1, leg 3
Outer spine on exopod segment 1, leg 4
First abdominal somite
Cuticular membrane around antenna
11
12
Digitiform process on caudal ramus
Digitiform process on caudal ramus
1
2
3
4
5
6
subviridis from various localities around India (Figure 16). D. amplectens had previously been found
on other grey mullet, including Liza argentea, Valamugil seheli and M. cephalus from Australia (Kabata,
1992). In India it occurs on the grey mullet, V. seheli
and as well as on hosts of other families, including
the Cichlidae (Etroplus maculatus (Bloch)), Gerreidae (Gerres steifer (Hamilton)), Hemiramphidae (Hyporamphus xanthopterus (Cuvier & Valenciennes)),
Chanidae (Chanos chanos (Forsskål)) and Megalop-
0 = present, 1 = absent
0 = present, 1 = absent
0 = shorter than wide, 1 = longer than wide
0 = absent or restricted to inner surface of first
endopodal segment, 1 = ensheathing at least first
endopodal segment but not inflated, 2 = extensive
inflated membrane ensheathing most of limb
0 = absent, 1 = present
0 = less than twice as long as ramus, 1 = more than
twice as long as ramus
idae (Megalos cyprinoides (Broussonet)) (Ho et al.,
1992).
D. varicoleus was recorded on Liza subviridis in
the present work, but it has been previously recorded
on two other grey mullet, L. tade and L. abu, from
India and Iraq, respectively (Ho et al., 1992; Ho et al.,
1996). D. mugilis was originally recorded from M.
cephalus in S. Africa (Oldewage & van As, 1988).
The remaining Dermoergasilus species are reported only from other host families. Three species
from Australia have been reported on different fam-
111
ilies of coastal fishes: D. intermedius on members
of the Plotosidae, Clupeidae and Percichthyidae (Kabata, 1992); D. acanthopagri on Sparidae (Byrnes,
1986); and D. semicoleus on one species of the family Belonidae (Cressey & Collette, 1970). D. coleus
was known from three species of belonids from the
Philippines (Cressey & Collette, 1970).
Dermoergasilus species are currently known to
utilise 27 species of fishes representing 11 different
host families (Figure 17B). Such a wide range of
hosts reflects a low degree of host-specificity both
at the family and at the species level. Its distribution is confined to the Old World, extending through
the Palaearctic, Oriental, Australasian and Ethiopian
regions.
Phylogenetic relationships of Dermoergasilus
species
The phylogenetic analysis was based on a data-matrix
(Table 1) of 12 morphological characters (Table 2)
and 11 species (including the outgroup). The typespecies of Ergasilus, E. sieboldi Nordmann, was
chosen as the outgroup. The analysis was performed
using PAUP 3.1.1. and the options employed were
BRANCH AND BOUND search, with characters set
as IRREVERSIBLE-UP. The analysis generated a single tree with branch length = 18, CI = 0.722 and f
value = 16.
The single tree (Figure 17A) shows three unresolved trichotomies, but the ingroup, Dermoergasilus,
is defined as monophyletic by the presence of the digitiform process on the caudal ramus. The first offshoot
from the main lineage is D. curtus, which lacks the
loose cuticular membrane ensheathing at least the first
endopodal segment of the antenna in all other species.
In D. curtus the loose cuticle is restricted to a small
region on the inner surface of that segment. Next to
diverge from the main clade, at an unresolved trichotomy, are D. semicoleus and D. intermedius. Both
these species have loose membrane ensheathing the
first endopodal segment of the antenna, but it is not
as extensive or inflated as in all remaining species.
These two species also retain a barrel-shaped genital double-somite in the female and lack the elongate
genital double-somite that characterises the more derived species, from D. amplectens to D. mugilis. The
species of this main clade share the following two apomorphies, an elongate genital double-somite and the
presence of a well-developed membrane ensheathing
the antenna. Within this derived clade the D. acanthopagri to D. mugilis group is defined by the elongate
first free abdominal somite. D. amplectens and D.
semiamplectens both retain the short first free abdominal somite. The D. coleus-D. mugilis group is defined
by the loss of the outer spine on the first exopodal
segment of legs 2 to 4. The D. varicoleus-D. longiabdominalis group is defined by the digitiform process
on the ramus being more than twice as long as the
caudal ramus. According to the tree topology, this apomorphy also arose independently in D. coleus and in
D. semicoleus.
A simple host summary cladogram for Dermoergasilus (Figure 17B) is produced by replacing each
species name with the host families utilised. Five of
the species of Dermoergasilus included in the main
clade occurred on members of the Mugilidae (D.
amplectens, D. semiamplectens, D. varicoleus, D. longiabdominalis and D. mugilis). The clustering in a
single clade suggests that their common ancestor may
have already colonised mugilids as hosts. Further, the
utilisation of a mugilid as host by the most plesiomorphic Dermoergasilus species, D. curtus, may be interpreted as evidence that the ancestor of Dermoergasilus
ocurred on a mugilid host. Indeed, El-Rashidy (1999,
figure 6.27) inferred that the genus Dermoergasilus
belonged to a much larger clade which also included
an as yet undescribed new species of Ergasilus (referred to under manuscript name of E. extensus in
El-Rashidy, 1999), Nipergasilus parabora El-Rashidy
& Boxshall and Nipergasilus bora (Yamaguti). The
members of this larger clade occurred primarily on
mugilid hosts. It is inferred here that Dermoergasilus
species, such as D. acanthopagri and D. coleus, which
occur on other families of coastal fishes (e.g. Sparidae and Belonidae) have probably switched hosts
secondarily from the Mugilidae.
Acknowledgements
The authors would like to thank Mark Wilkinson for
his valuable advice and help with the phylogenetic
analysis. We are also grateful to Rony Huys for his
advice and to Sophie Conroy Dalton for her assistance with the drawings. Many thanks also to Jim
Chimonides for his help with the distribution map and
to Chad Walter (USNM, Smithsonian Institution) who
made some type-material available.
112
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