The Lichenologist 45(6): 739–762 (2013)
doi:10.1017/S0024282913000522
6 British Lichen Society, 2013
The lichen genera Cryptothecia, Herpothallon and Helminthocarpon
(Arthoniales) in the Galapagos Islands, Ecuador
Frank BUNGARTZ, Valeria Leonor DUTÁN-PATIÑO & John A. ELIX
Abstract: As part of a comprehensive biodiversity inventory of Galapagos lichens, all species in
two closely related genera, Cryptothecia and Herpothallon, are reviewed. Both genera are superficially
similar, ecorticate, cottony-byssoid crusts and are unusual insofar as their asci do not develop in
distinct ascomata, but instead within ascigerous areas or even solitary inside pseudisidia. Species of
Herpothallon typically have an I-- medulla and are covered in ecorticate pseudisidia; only a single
species is known fertile. Cryptothecia is characterized by ascigerous areas with loosely aggregated asci.
In some species the asci are isolated, but others have closely aggregated asci embedded in a hyphal
matrix with some carbonization, perhaps indicating preliminary stages towards a development of true
ascomata. Lirellate ascomata of the enigmatic, monotypic Helminthocarpon leprevostii show similarity
with these ascigerous areas, especially of C. darwiniana and C. galapagoana, two species newly described
here. Both also have similar asci and ascospores. As previously suggested, Helminthocarpon should
thus not be included in Graphidaceae, but it belongs in Arthoniales, possibly in Arthoniaceae or
Opegraphaceae. A key to all species and brief descriptions are provided. Two of the three Cryptothecia
species and two of the seven Herpothallon species reported here are new to science. All records apart
from Herpothallon rubrocinctum are new to Galapagos and Ecuador.
Key words: Census of Galapagos Biodiversity, Galapagos Lichen Inventory, taxonomy, lichenized
Ascomycota
Accepted for publication 10 July 2013
Introduction
In the tropics the genera Herpothallon and
Cryptothecia are common and widely distributed crustose genera of lichenized fungi.
Both are closely related, forming conspicuous cottony-byssoid crusts with trentepohlioid photobionts and asci not developing inside true ascomata. Species of Herpothallon
were, until recently, included within Cryptothecia, but have been segregated by Aptroot
et al. (2009): almost all species of Herpothallon are sterile, only one species, H. fertile, is
F. Bungartz and V. L. Dután-Patiño: Biodiversity Assessment, Charles Darwin Foundation (AISBL), Puerto
Ayora, Santa Cruz, Galapagos, Ecuador; postal address:
Avenida Juan Gonzales N35–26 y Juan Pablo Sanz,
Quito, Ecuador. Email: frank.bungartz@fcdarwin.org.ec
V. L. Dután-Patiño: Universidad del Azuay, Cuenca,
Ecuador.
J. A. Elix: Research School of Chemistry, Australian National University, Canberra, A.C.T. 0200, Australia.
known to produce isolated asci within pseudisidia and ascospores with straight, not
curved septa (as is characteristic for Cryptothecia). All species typically have abundant
pseudisidia, i.e., isidioid outgrowths that, despite their similarity to true isidia, lack internal differentiation and have no distinct cortex. In many species pycnidia develop inside
these pseudisidia. The medulla of all but
one species does not react with iodine; only
H. philippinum has a patchy I+ blue medulla.
Cryptothecia can generally be distinguished
because species in that genus generally do
not form pseudisidia (although granules
may be present on the thallus surface); instead their thalli are typically fertile with asci
loosely dispersed or closely aggregated within ascigerous areas. Frequently these areas
develop into pustulate outgrowths, and the
specimens have a medulla that, at least in
parts, reacts blue with iodine.
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THE LICHENOLOGIST
In the Galapagos, species of both genera
are common and abundant in the humid
forests of the highlands, some venture into
the transition zone, and only few occur in
the dry zone. With its bright carmine red pigmentation the ‘Christmas Lichen’ Herpothallon rubrocinctum is among the most conspicuous common lichens of the humid Galapagos
highlands. Equally abundant and almost as
conspicuous are the large white patches of
Cryptothecia striata.
Despite being characteristic elements of
the Galapagos lichen vegetation, not many
species have been reported until recently.
Weber’s classical checklists (Weber 1986,
Weber 1993, Weber & Beck 1985, Weber &
Gradstein 1984, Weber et al. 1977) mention
only two: Herpothallon rubrocinctum (as H.
sanguineum) and Chiodecton effusum Fée (a
record based on misidentifications of Sclerophyton murex and Cryptothecia darwiniana).
This may not be surprising because at first
glance the thalli of both genera have few
diagnostic characters and analysis of their
secondary chemistry is often necessary to
distinguish the species.
As a result of the Galapagos Lichen Inventory (Bungartz et al. 2010b) a total of ten species is reported here. These are included in
our regularly updated online checklist (Bungartz et al. 2010a) as part of a large scale biodiversity inventory by the Charles Darwin
Foundation with the long-term goal to establish a comprehensive Census of Life for the
Galapagos Islands (Yánez et al. 2011).
During the revision of historic specimens
and recently collected material, it soon became evident that specimens of Cryptothecia
darwiniana in particular, but to some extent
also C. galapagona, both newly described, are
characterized by an unusual anatomy and
morphology. The specimens share some
characteristics with the enigmatic Helminthocarpon leprevostii, a lichen of uncertain taxonomic position. Because of its lirellate ascomata this species is still often listed as a
member of the Graphidaceae (Kirk 2011,
Kirk et al. 2011). Nevertheless, it was not
included in our treatment of Galapagos
Graphidaceae (Bungartz et al. 2010c), because
we agree with Staiger (2002) and Aptroot
(1999) that the genus belongs in the Artho-
Vol. 45
niales, not Ostropales. Aptroot (1999) suggested including Helminthocarpon within the
Arthoniaceae, but the family concept within
the Arthoniales was changed considerably in
the most up-to-date revision by Ertz & Tehler
(2011) and they did not include Helminthocarpon in their analysis. Its taxonomic position thus remains unclear.
Here we present short descriptions of all
Galapagos species of Cryptothecia and Herpothallon with an identification key and we
discuss how Helminthocarpon leprevostii shows
similarities to these genera and other Arthoniales, evidence that strongly suggest that it
belongs either in the Arthoniaceae or the Opegraphaceae sensu Ertz & Tehler (2011).
Methods
The Galapagos Archipelago comprises more than 123
oceanic islands, islets and large rocks that emerged
from the sea as a result of volcanic hot spot activity; fourteen islands are somewhat arbitrarily recognized because
of their size as the principal islands (Snell et al. 1996).
The Galapagos climate is unusually dry, with a hot and
cool season and prevailing winds from the south and
south-east (Trueman & d’Ozouville 2010). Five principal
vegetation zones can be distinguished: coastal, dry, transition, humid, and high altitude dry zone (Bungartz et al.
2010c, Tye et al. 2002).
As part of the Galapagos Lichen Inventory the following islands have been visited; all vegetation zones were
surveyed: Isabela (Volcán Sierra Negra, Volcán Alcedo,
Volcán Darwin), Santiago (incl. Rabida, Bartolomé),
Santa Cruz (incl. Santa Fé, Plaza Sur, Plaza Norte,
Roca Gordon, Pinzón), Pinta, Española, Floreana, and
San Cristóbal.
Herbarium collections of the inventory are deposited
at CDS; specimens from historic collections have also
been examined (COLO, CAS, FH, H, S, B). All specimens were examined with a Zeiss Stemi DV4 dissecting
microscope and a Zeiss Imager A1 compound microscope equipped with differential interference contrast.
Macrophotographs were taken with a Nikon D300, 62
mm Nikkor Micro Lens and R1C1 macro flash directly
in the field, or using a Novoflex macro-table to take images of herbarium specimens; for photographic magnifications higher than 1:1 an extension tube or Novoflex
bellows was used. For microphoto the compound microscope is equipped with a phototube for the Nikon
D300. Photographs in the laboratory were taken with
Nikon Camera Control Pro 2; all photo were databased
with the program IDimager 5 using the Darwin Core
XML schema to embed collection and identification
information as XMP metadata (http://owl.phy.queensu.
ca/~phil/exiftool/TagNames/DarwinCore.html). Photos
were processed with Photoshop CS4.
2013
Arthoniales in the Galapagos—Bungartz et al.
Secondary metabolites were examined from a selection of specimens using standardized thin-layer chromatography (Orange et al. 2001, 2010); instead of the
conventional upright TLC tanks a horizontal HPTLC
developmental chamber was used (Arup et al. 1993).
TLC plates were documented with a Nikon D300 digital
camera. Photographs were taken immediately after running the solvent, in long wave (366 nm) and short wave
(254 nm) UV light, before applying 10% H2SO4. After
H2SO4 treatment and charring in a laboratory oven for
741
approx. 8 min at 110 C a second set of photographs in
visible light and short wave UV (254 nm) were taken.
Due to the large number of specimens examined, collection data for only few representative examples are included here. Where available, at least one specimen per
surveyed island (and, in the case of Isabela, the island’s
different main volcanoes) has been listed. Detailed collection information for all Galapagos specimens used in
this study can be downloaded from the CDF Collections
Database online at http://www.darwinfoundation.org/
datazone/collections/.
Key to the species of Herpothallon and Cryptothecia from the Galapagos
1
Thallus ecorticate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Thallus corticate, with a dull to eshiny, beige surface and beige to white, short
and broad, elirellate ascomata, lacking isidia or soredia; medulla ILugol’s-throughout . . . . . . . . . . . . . . . . . . . . . . . . . . Helminthocarpon leprevostii
2(1)
Thallus ecorticate, of loosely interwoven hyphae, typically with a cottony surface;
the thallus centre densely covered by pseudisidia (i.e., ecorticate isidia-like outgrowth that in a few species may be inhabited by a single pycnidium or a single
ascus); medulla ILugol’s-- throughout, very rarely reacting ILugol’s+ blue in parts*
[Herpothallon] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
*records of Galapagos specimens reported as Herpothallon philippinum by Aptroot et al. (2009) cannot be
confirmed here, but the medulla of the newly described H. hypostictium also reacts in parts weakly I+
blue.
Thallus ecorticate, of loosely or densely interwoven hyphae, frequently with large
quantities of crystals and then with a shiny, shimmery surface that almost appears
corticate; rarely with scarce, loose granules or soredia, but all species generally
lacking pseudisidia; thallus with irregular pustules or cushion-shaped outgrowths
that contain few, scattered asci (ascigerous areas); medulla, or at least in parts,
distinctly reacting ILugol’s+ blue* [Cryptothecia] . . . . . . . . . . . . . . . . . . . . . . . . 9
3(2)
Thallus and prothallus with a bright to rust red, K+ purple pigment; pigment occasionally scarce, but always present . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Thallus and prothallus lacking reddish pigment, K not reacting purple . . . . . . . . . 5
4(3)
Thallus pale greenish grey, surrounded by a wide, bright carmine red prothallus,
bearing bright carmine red pseudisidia in its centre; pseudisidia not known to contain pycnidia . . . . . . . . . . . . . . . . . . . . . . . . . . Herpothallon rubrocinctum
Thallus pale greyish to greenish white, prothallus concolorous or, at least in parts,
distinctly rust red, pseudisidia concolorous with the thallus, but always with few,
conspicuous, scattered rust red, needle-shaped pigment crystals; pseudisidia occasionally containing a single pycnidium . . . . . . Herpothallon rubroechinatum
Thallus forming fibrous strands of eloosely interwoven hyphae on a whitish arachnoid prothallus bearing coarse, granular pseudisidia . . . . . . . . . . . . . . . . . . . . 6
Thallus thick, dense, cottony or thin and arachnoid, but then not forming distinct,
fibrous hyphal strands; pseudisidia fine, powdery to distinctly cylindrical. . . . . . 7
Thallus K--, P-- (perlatolic acid); on bark. . . . . . . . . . . . Herpothallon granulare
Thallus K+ yellow, P+ yellow (brialmontin 1 & 2, confuentic acid); on rock . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Herpothallon saxorum
5(3)
6(5)
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THE LICHENOLOGIST
Vol. 45
7(5)
Thallus P+ bright golden yellow (psoromic acid); with distinct, elongate cylindrical
pseudisidia (occasionally containing a single pycnidium) . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Herpothallon echinatum
Thallus P-- (lacking psoromic acid); pseudisidia granular to elongate cylindrical (not
containing pycnidia) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
8(7)
Thallus thin, loosely arachnoid to dense and compact; prothallus white; with hypostictic and hyposalazinic acid; medulla in parts ILugol’s+ weakly blue. . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Herpothallon hyposticticum
Thallus thin, loosely arachnoid; prothallus conspicuously brown; with confluentic
acid only; medulla ILugol’s-- thoughout. . . . . . Herpothallon aff. confluenticum
9(2)
Thallus UV+ bright orange (always with xanthones), lime yellow to pale whitish yellow;
surface smooth, compact, eshiny . . . . . . . . . . . . . Cryptothecia darwiniana
Thallus UV-- (lacking xanthones), white to pale grey or beige with storage, when fresh
sometimes with a egreenish tinge, never yellowish; surface cottony or densely
granular, not shiny; . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
10(9) Thallus C+ bright red, P--; surface with byssoid pustules or byssoid dots, but always
lacking ‘soredia’ (pseudoisidiate granules). . . . . . . . . . . Cryptothecia striata*
Ascigerous areas confined to conspicuous, discrete, broad, cushion-shaped pustules, not arranged in
distinct striae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . pustulate morphotype
Ascigerous areas in byssoid dots, when well developed typically arranged in distinct radial lines (striae) . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . striate morphotype
Thallus C--, P+ yellow; surface densely covered by coarse ‘soredia’ (pseudisidiate
granules) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cryptothecia galapagoana
Species Descriptions
Cryptothecia Stirt.
Proc. Roy. Philos. Soc. Glasgow 10: 164 (1876)
Cryptothecia darwiniana Bungartz &
Elix sp. nov.
MycoBank no. 804737
Similar to Cryptothecia assimilis and C. lichexanthonica
but with confluentic acid in addition to lichexanthone;
ascospores much larger than those of C. lichexanthonica
and only slightly larger than those of C. assimilis; of the
8 ascospores several often abortive.
Type: Ecuador, Galapagos Islands, Isla Floreana, Ladera N del Cerro Ventanas, 1 16 0 1200 S, 90 25 0 4900 W,
alt. 400 m, zona húmeda, sobre corteza de tallo de Bursera graveolens, 27 iii 2006, Simbaña 556 (CDS 32392—
holotype).
(Fig. 1)
Thallus corticolous or lignicolous, delimited
by a distinct byssoid prothallus of white to
brownish, radiating hyphae; thallus surface
smooth, shiny, ecorticate, yellowish white,
with storage intensifying lemon yellow, occa-
sionally with sparse granular ‘soredia’ (pseudisidiate granules); medulla white, densely
filled with minute colourless crystals (soluble
in KOH, not forming colourless, needleshaped crystals in 25% H2SO4).
Ascigerous areas developing within roundish, broad, lemon yellow, pruinose, irregular
pustules with a eroughened surface which
at maturity breaks open; asci bitunicate-fissitunicate, broadly pyriform to globose, with a
short stalk, a thick wall (c. 21 mm) and thick
tholus with small ocular chamber; occurring
isolated within thalline pustules, entangled
by strongly ramified and anatomizing, KI+
violet-blue paraphysoids; one to few asci
with age encapsulated by a brownish pigmentation, eventually becoming carbonized forming ‘locules’ that are irregularly arranged or develop into striae, when eroded
at their surface these ascigerous pustules
thus appearing similar to ramified, carbonized lirellae; ascospores hyaline, K+ pale olivaceous, ovoid, muriform, with curved septa,
(50–)60–85(–95) (22–)32–40(–48) mm,
2013
Arthoniales in the Galapagos—Bungartz et al.
743
Fig. 1. Cryptothecia darwiniana. A, general thallus aspect with brownish prothallus delimiting two thalli (holotype);
B, flattened ascigerous pustules (Bungartz 5033); C, strongly swollen ascigerous pustules (Bungartz 5973); D section
of ascigerous pustule with carbonized locules inside, locules often in irregular ramified lines (Aptroot 65296);
E, ovoid, muriform ascospores, principal septa curved (holotype,); F, close-up of ovoid ascospore (holotype). Scales:
A & B ¼ 5 mm; C & D ¼ 2 mm; E ¼ 70 mm; F ¼ 35 mm.
744
THE LICHENOLOGIST
typically 8 per ascus, but frequently 1–2(–3)
spores aborted, these asci thus remaining
(5–)6–7-spored.
Conidiomata not observed.
Spot tests and chemistry. P--, K--, C--, KC--;
UV+ bright orange; medulla ILugol’s+ deep
blue; confluentic acid, lichexanthone.
Distribution and ecology. Currently known
only from the Galapagos and possibly endemic; common throughout the dry zone
and lower transition zone, currently known
only from bark of native and endemic tree
species, most commonly Bursera graveolens
and Erythrina velutina, in sunny, wind- and
rain-exposed habitats.
Notes. Two other Cryptothecia species with
a UV+ bright yellow to orange fluorescence
are currently known: Cryptothecia assimilis
Makhija & Patw. described from India, and
C. lichexanthonica E. L. Lima et al. described
from Brazil. For C. assimilis Makhija &
Patwardhan (1994 p. 63) mention a lichen
substance that, when analyzed with TLC,
gives a ‘‘UV+ bright yellow fluorescence;
unknown yellow spot at atranorin (UV+
orange pink fluo.)’’. Lima et al. (2013) state
that both C. lichexanthonica and C. assimilis
contain lichexanthone, a substance also present in C. darwiniana.
The three species are very similar: according to Lima et al. (2013) C. lichexanthonica
has asci that are globose and not pedicellate,
and its ascigerous areas are generally much
smaller than those of C. assimilis. The ascospores of C. lichexanthonica (55–75 22–28
mm) are also smaller than those of C. assimilis
(66–83 25–33 mm). Both species generally
have 8 spores per ascus. The Galapagos
specimens of C. darwiniana have distinctly
pedicellate asci, their ascigerous areas vary
considerably in size, but are generally larger
than those of both of the other species, and,
when very well developed, form distinct pustules. With ascospores 60–85 32–40 mm,
C. darwiniana has the largest ascospores of
the three, though only slightly broader than
those of C. assimilis. Cryptothecia darwiniana
is the only species to contain confluentic
acid in addition to lichexanthone.
Vol. 45
During our studies, the identity of C.
darwiniana remained unresolved for a considerable time. Weber (1986) first reported
Chiodecton effusum Fée for the Galapagos, a
species cited as Syncesia effusa (Fée) Tehler
by Elix & McCarthy (1998). The thalli are
indeed superficially similar to those of Syncesia effusa, especially when the surface of
the ascigerous pustules becomes abraded.
Ascospores of Syncesia, however, are slender,
fusiform, 3-septate, whereas those of Cryptothecia are broad, oblong to ovoid and muriform.
The record by Weber (1986) of Chiodecton
effusum is even more confusing and a revision
of the COLO specimens confirmed that this
material was not Cryptothecia darwiniana. Instead most specimens of C. effusum in COLO
are misidentifications of Sclerophyton murex
Egea & Torrente ex Sparrius, and one specimen is Syncesia graphica (Fries) Tehler.
Reports of Syncesia effusa from Galapagos
are thus erroneous, no material of that species was found in COLO and it has not been
collected during our recent inventory.
Selected specimens examined. Ecuador: Galapagos: Isla
Española, trail from Bahı́a Manzanillo on the N-coast
of the island to the highest point, 1 21 0 4100 S,
89 41 0 5700 W, alt. 48 m, dry zone, on bark, 2010, Bungartz 9086 (CDS 45904); Bungartz 9125 (CDS 45943).
Isla Floreana, along trail going to Post Office Bay off
the dirt road between highlands and Puerto Velasco
Ibarra, at Laguna Seca, 1 15 0 3100 S, 90 26 0 2700 W, alt.
206 m, dry zone, on bark, 2011, Bungartz 9530 (CDS
46813); inside the crater of Cerro Laguna at E-side of
island, on W-exposed slope, 1 16 0 1200 S, 90 23 0 1700 W,
alt. 245 m, transition zone, on bark, 2011, Bungartz
9941 (CDS 47310). Isla Pinta, along the trail up to the
summit from the S-coast, 0 34 0 300 N, 90 44 0 5600 W, alt.
233 m, transition zone, on bark, 2007, Bungartz 5973
(CDS 33651). Isla Pinzón, along the trail going
up from Playa Escondida, 0 36 0 1000 S, 90 40 0 100 W, alt.
254 m, dry zone, on bark, 2006, Bungartz 3639 (CDS
27457), Aptroot 64116 (CDS 30677). Isla San Cristóbal, north-western foothills of Media Luna, inland from
the NW-coast, 0 43 0 4100 S, 89 18 0 4400 W, alt. 75 m, dry
zone, on bark, 2007, Bungartz 6177 (CDS 34389). Isla
Santa Cruz, above Mina Granillo Rojo, on the N-side
of the island, 0 37 0 400 S, 90 21 0 5700 W, alt. 600 m, transition zone, 2008, Clerc 08-27 (CDS 39881); along
the road from Bellavista to El Garrapatero, c. 4 km W
from the campsite of the National Park, 0 40 0 3800 S,
90 14 0 5400 W, alt. 159 m, dry zone, on bark, 2006, Bungartz 3569 (CDS 27368); along the road from Bellavista
to El Garrapatero, c. 4 km W from the campsite of the
National Park, 0 40 0 3800 S, 90 14 0 5400 W, alt. 159 m, dry
2013
Arthoniales in the Galapagos—Bungartz et al.
zone, on bark, 2006, Aptroot 63979 (CDS 30539). Isla
Santiago, c. 5 km inland from the E-coast, e at the same
latitude as Bahı́a Sullivan, 0 16 0 5200 S, 90 37 0 1700 W, alt.
175 m, dry zone, on bark, 2006, Bungartz 5033 (CDS
29246, ), c. 7 km inland from the E-coast, e at the same
latitude as Bahı́a Sullivan, 0 17 0 400 S, 90 38 0 2100 W, alt.
190 m, dry zone, on bark, 2006, Bungartz 5088 (CDS
29301); en el parte sureste de la isla, 0 17 0 500 S,
90 38 0 3500 W, alt. 160 m, zona seca, sobre corteza,
2006, Nugra 114 (CDS 32768). Isla Isabela, Volcán
Alcedo, along the trail going up the E-slope, basalt
rubble field to the SE-side of the trail and the barranco,
0 24 0 600 S, 91 2 0 5300 W, alt. 530 m, dry zone, on bark,
2006, Aptroot 64914 (CDS 31493); alt. 434 m, on bark,
2006, Aptroot 64981 (CDS 31561); plain at the base of
the outer E-exposed slope, along the trail going up to
the rim, 0 24 0 4700 S, 91 4 0 1200 W, alt. 768 m, transition zone, on bark, 2006, Aptroot 65184 (CDS 31768);
Volcán Sierra Negra, area around the Muro de las
Lagrimas, ca. 5 km W of Puerto Villamil, 0 57 0 5400 S,
91 0 0 4900 W, alt. 81 m, dry zone, on bark, 2008, Bungartz
8399 (CDS 41045); Cerro Orchilla, ca. 4 km W of
Puerto Villamil, 0 57 0 4700 S, 91 0 0 2700 W, alt. 56 m, dry
zone, on bark, 2008, Bungartz 8473 (CDS 41119).
Cryptothecia galapagoana Bungartz &
Elix sp. nov.
MycoBank no. 804738
Cryptothecia with pustulate ascigerous areas, typically
covered by coarse pruina and granular soredia, reacting
P+ yellow, K+ yellow, containing confluentic, 2’-Omethylperlatolic, and 2’-O-methylmicrophyllinic acids.
Type: Ecuador, Galapagos Islands, Isla Pinzón, along
the trail going up from Playa Escondida, SW-slope of
the top, 0 36 0 3600 S, 90 40 0 1100 W, alt. 310 m, transition
zone, dry transition zone with Prosopis juliflora, Opuntia
galapageia ssp. macrocarpa, Croton scouleri, Cordia lutea,
Scalesia baurii ssp. baurii, on Opuntia, 16 ii 2006, Aptroot
64075 (cds 30636—holotype).
(Fig. 2A–C)
Thallus corticolous, delimited by a white
to brownish, compact prothallus of densely
interwoven hyphae; thallus surface cottony,
ecorticate, with a dull eroughened surface,
pale beige to yellowish or greyish white, typically abundantly covered with granular ‘soredia’ (pseudisidiate granules); medulla white,
densely filled with minute colourless granules
and sparse calcium oxalate crystals (insoluble
in KOH, forming colourless, needle-shaped
crystals in 25% H2SO4).
Ascigerous areas developing within yellowish
pale, irregular to subglobose coarsely pruinose pustules, which at maturity break open
and are generally densely covered in sorediate
745
granules; asci bitunicate-fissitunicate, pyriform to broadly pyriform, with a short stalk,
a thick wall (c. 20 mm) and thick tholus
with small ocular chamber, forming isolated
to loosely grouped within the thalline pustule, very loosely entangled by few, IK+
violet-blue paraphysoids; one to several asci
with age encapsulated by a brownish pigmentation, eventually becoming carbonized
within ‘locules’, visible as black dots with
erosion of the pustule surface, dots aggregated within the pustules, but not ‘lirellate’;
ascospores hyaline, K+ pale olivaceous, ovoid,
muriform, with curved septa, (13–)14–
175(–19) (65–)75–95(–10) mm, 8 per
ascus.
Conidiomata not observed.
Spot tests and chemistry: P+ yellow, K+ yellow, C--, KC--; UV--; medulla ILugol’s+ deep
blue; confluentic, 2’-O-methylperlatolic, and
2’-O-methylmicrophyllinic acids; occasionally possibly also with traces of gyrophoric
acid (but all specimens C--).
Distribution and ecology. Currently known
only from Galapagos and very likely endemic;
a rare species, known only from the bark of
the native tree Bursera graveolens and cactus
pads (Opuntia galapageia ssp. macrocarpa);
in sunny, wind- and rain-exposed habitats.
Notes. Cryptothecia galapagoana is a very
rare species of which only three specimens
have so far been collected. A minute piece of
the species was first identified by R. Lücking
as C. evergladensis Seavey based on similar
ascospores and the P+ yellow reaction of the
thallus fragment. Even when TLC did not
confirm psoromic acid, it was assumed that
the wrong material must have been accidentally analyzed. Repeating the TLC would
have destroyed what was left of the specimen
and only when a much larger specimen was
subsequently discovered and analyzed was it
possible to confirm that the material indeed
lacked psoromic acid. This much larger specimen, selected here as the type, clearly shows
that the species is not only chemically distinct,
but that it also differs morphologically from
C. evergladenis.
746
THE LICHENOLOGIST
Vol. 45
Fig. 2. A–C, Cryptothecia galapagoana; A, general thallus aspect with ascigerous pustules covered by granules
(holotype); B, broadly pyriform asci with ovoid, muriform ascospores (Aptroot 64081); C, apices of two asci with
thick bitunicate-fissitunicate tholus with small ocular chamber, spores ovoid, muriform with faintly curved septa
(Aptroot 64081). D–F, Cryptothecia striata; D, general growth aspect of several merged thalli (Bungartz 4314); E,
thallus close-up, white prothallus with pale yellowish discoloration, ascigerous areas punctiform, radiating in distinct
striae towards the margin, merging in the centre (Nugra 887); F, close-up of thallus surface with radiating striae
of small pustulate ascigerous areas and a discoloration patch from C+ red spot testing (Aptroot 64322). Scales:
A ¼ 1 mm; B ¼ 25 mm; C ¼ 15 mm; D ¼ 5 mm; E & F ¼ 3 mm.
2013
Arthoniales in the Galapagos—Bungartz et al.
The thallus of C. galapagoana is quite unusual, because it is abundantly covered in
sorediate granules that have a ecoarse pruinose appearance due to an abundance of
colourless granules (?crystals) throughout the
thallus. These granules not only appear on
the thallus surface itself, but typically cover
the ascigerous pustules.
Additional specimens examined. Ecuador: Galapagos:
Isla Santa Cruz, on the North side of the island, along
the dirt road to the ash quarry Mina Granillo Rojo, 0 36 0
5600 S, 90 22 0 300 W, alt. 570 m, lower transition zone, on
bark of Bursera, 2006, Aptroot 64600 (CDS 31173). Isla
Pinzón, along the trail going up from Playa Escondida,
SW-slope of the top, 0 36 0 3600 S, 90 40 0 1100 W, alt. 310,
transition zone, on Opuntia, 2006, Aptroot 64081 (CDS
30642).
Cryptothecia striata G. Thor
Bryologist 31: 278 (1991)
(Figs. 2D–F, 3A–C)
Thallus corticolous, rarely saxicolous, delimited by a distinct byssoid prothallus of
white, radiating hyphae; thallus surface cottony,
ecorticate, greenish grey to greyish white, with
storage beige, lacking soredia; medulla white,
densely filled with minute colourless granules
and sparse calcium oxalate crystals (insoluble
in KOH, forming colourless, needle-shaped
crystals in 25% H2SO4).
Ascigerous areas developing in the thallus
centre as small, byssoid dots that soon merge
into distinctly radiating striae, rarely not forming striae, but merging into large pustular outgrowths (not true soredia); asci bitunicatefissitunicate, broadly pyriform to globose,
with a short stalk, a moderately thickened
wall (c. 4–6 mm) and thick tholus with small
ocular chamber, individual asci irregularly
dispersed, isolated to loosely grouped, not
closely aggregating, entangled by few, IK+
violet-blue paraphysoids, occasionally surrounded by diffuse brownish pigment, not
carbonized; ascospores hyaline, K--, ovoid to
oblong, occasionally slightly bent, muriform,
with curved septa, (46–)55–70(–80) (19–)
23–29(–37) mm, 1(–2) per ascus.
Conidiomata not observed.
747
Spot tests and chemistry. P--, K--, C+ bright
red, KC+ bright red; UV-- (pale yellowish
green); medulla ILugol’s+ deep blue; gyrophoric and/or lecanoric acid, etraces of
atranorin.
Distribution and ecology. Cosmopolitan, new
to Galapagos and Ecuador; the most common
Cryptothecia species in Galapagos, often very
abundant, especially in the humid and upper
transition zone, rarely also in the dry zone,
on a wide range of mostly native, but also introduced tree species, typically in eshaded
and sheltered habitats.
Notes. Aptroot et al. (2009) report two
specimens collected in Galapagos as Herpothallon philippinum. We were unable to locate
specimen Aptroot 54328 in CDS and were
thus unable to examine the specimen and
confirm its identification. The second specimen cited as Aptroot 64330 (CDS accession
no. 30895) contains psoromic acid and thus
belongs to Herpothallon echinatum. Reports
of H. philippinum therefore cannot be confirmed for Galapagos.
Morphotypes of thalli that do not form
very distinct striae, but are instead abundantly covered in pustular outgrowths within
which the asci develop have previously erroneously been reported as C. punctosorediata
(Bungartz et al. 2010b). Sparrius & Saipunkaew (2005) emphasize that in C. punctosorediata the thallus does not react with C, but
that only the soredia contain gyrophoric acid
and thus react C+ bright red. For C. punctosorediata they also report asci with six to eight
ascospores.
The pustules of the Galapagos material
previously identified as C. punctosorediata are
not true soredia, but they clearly represent
the ascigerous regions in which asci with
one, rarely two, never eight ascospores develop. Thalli and pustules contain lecanoric
acid and both react distinctly C+ bright red;
this reaction is clearly not confined to the
pustules only. Finally, unlike as reported for
C. punctosorediata by Sparrius & Saipunkaew
(2005) the material does not fluoresce conspicuously white under UV light.
748
THE LICHENOLOGIST
Selected specimens examined. Ecuador: Galapagos: Isla
Floreana, Asilo de la Paz, Cerro Wittmer, road to Post
Office Bay, 1 18 0 4100 S, 90 27 0 400 W, humid zone, on
bark, 2010, Hillmann GAL-82 (CDS 44908); c. 1 km S
of Cerro Verde, 1 18 0 3400 S, 90 25 0 1900 W, alt. 299 m,
transition zone, on bark, 2011, Yánez 1954 (CDS
48306); c. 200 m from the northern limit of the Primavera Farm in the highlands of the island, 1 18 0 4800 S,
90 26 0 700 W, alt. 339 m, humid zone, on bark, 2011,
Bungartz 10051 (CDS 47446); caldera of Cerro Pajas,
old trail in the caldera, 1 17 0 4800 S, 90 27 0 2100 W, humid
zone, on bark 2010, Hillmann GAL-13, 28, 33, 38, 40,
46, 49, 51, 53, 57 (CDS 44776, 44853, 44796, 44801,
44803, 44793, 44852, 44816, 44818, 44827). Isla
Pinta, along the trail up to the summit from the S-coast,
0 34 0 3100 N, 90 45 0 600 W, alt. 388 m, humid zone, on
bark, 2007, Bungartz 5844 (CDS 33519); 0 34 0 5700 N,
90 45 0 1100 W, alt. 579 m, humid zone, on bark, 2007,
Bungartz 5766 (CDS 33438); 0 34 0 3900 N, 90 45 0 700 W,
alt. 436 m, humid zone, on bark, 2007, Bungartz 5806
(CDS 33479). Isla Pinzón, E-facing side of a valley on
the W-slope of the highest mountain, 0 36 0 4900 S,
90 40 0 1400 W, alt. 294 m, transition zone, on bark, 2006,
Bungartz 3650 (CDS 27468). Isla San Cristóbal, W of
the cemetery of El Progresso at the border of the National
Park, 0 54 0 4500 S, 89 34 0 3400 W, alt. 170 m, transition
zone, on bark, 2008, Bungartz 8559 (CDS 41205); El
Chino in the southern higher part of the island,
0 54 0 4200 S, 89 27 0 1500 W, alt. 220 m, humid zone,
agricultural area, on bark, 2007, Bungartz 6771 (CDS
35022). Isla Santa Cruz, abandoned farm along the
northern part of the loop road from Bellavista to Garrapatero, 0 40 0 5800 S, 90 18 0 3100 W, alt. 255 m, humid
zone, on bark of Cedrela, 2006, Aptroot 64322 B (CDS
44663), Aptroot 64329 (CDS 30894), Aptroot 64322 A
(CDS 30887); above the quarry Mina Granillo Rojo, off
the main road to the channel, on the N-side of the
island, 0 37 0 600 S, 90 21 0 5900 W, alt. 617 m, transition
zone, on bark, 2007, Ertz, D. 11601 (CDS 36927). Isla
Isabela, Volcán Alcedo, outer SE-exposed slope, c.
25 km below the crater rim, 0 26 0 1300 S, 91 4 0 3300 W,
alt. 785 m, transition zone, on bark, 2006, Bungartz
4254 (CDS 28324), Bungartz 4239 (CDS 28309); Bungartz 4255 (CDS 28325); plain at the base of the outer
E-exposed slope, along the trail going up to the rim,
0 24 0 4700 S, 91 4 0 1200 W, alt. 768 m, transition zone, on
bark, 2006, Bungartz 4314 (CDS 28388), Aptroot
64866 (CDS 31443); Volcán Cerro Azul, along the
trail from Caleta Iguana to the first Caseta del Parque,
0 59 0 1400 S, 91 25 0 3900 W, alt. 300 m, transition zone, on
bark, 2012, Bungartz 10307 (CDS 52280), Bungartz
10309 (CDS 52282); lower half of path from the Caseta
del Parque to Caleta Iguana, 0 58 0 5200 S, 91 26 0 4100 W,
alt. 74 m, dry zone, on wood, 2012, Bungartz 10478
(CDS 52440); path from the fist Caseta del Parque to
Caleta Iguana, approx. a third of the way, 0 59 0 1000 S,
91 26 0 500 W, alt. 224 m, transition zone, on bark, 2012,
Spielmann 10641 (CDS 52008).
Helminthocarpon Fée
Essai Crypt. Exot., Suppl. (Paris): 156 (1837)
Vol. 45
Helminthocarpon leprevostii Fée
Essai Crypt. Exot., Suppl. (Paris): 156 (1837)
(Fig. 3C–F)
Thallus corticolous, rarely lignicolous, not
delimited by a distinct prothallus, in contact
with other thalli often forming a thin brown
line; thallus surface pale beige, corticate,
smooth and shiny; medulla white, densely
packed with minute crystals that dissolve
completely in KOH, lacking calcium oxalate
crystals (not forming colourless, needle-shaped
crystals in 25% H2SO4).
Ascomata developing as short, broad, swollen lirellae with a thick outer thalline layer
that at maturity breaks open along a wide,
irregular central slit; exciple flanks strongly
carbonized, but buried below thick thalline
layer; subhymenium and hypothecium hyaline
to pale brown; hamathecium of strongly ramified and anastomosing paraphysoids that
closely envelop the asci, KI+ blue; asci bitunicate-fissitunicate, cylindrical to narrowly
clavate, basally tapering into a stalk, immature ascus wall strongly thickened, with
maturity differentiating into two distinct
layers (c. 20–25 mm wide, basally thinner,
thickened towards the apex), KI+ pale blue
throughout, at the apex occasionally with a
minute, KI+ deep blue ring, ocular chamber
indistinct or absent; ascospores hyaline, K--,
elongate fusiform, muriform with curved
septa, (89–)120–150(–165) (22–)26–44
(–62) mm, 8 per ascus;.
Conidiomata not observed.
Spot tests and chemistry. P--, K--, C+ red,
KC+ red; UV-- (pale yellowish green); medulla ILugol’s--; lecanoric acid.
Distribution and ecology. Pantropical; new
to Galapagos, moderately common throughout the dry and lower transition zone on bark
of native and endemic trees, especially Bursera graveolens, Pisonia floribunda and Cordia
lutea; mostly in semi-shaded to shaded and
esheltered habitats, rarely also in moderately sunny and eexposed sites.
Specimens examined: Ecuador: Galapagos: Isla Española, along S-coast of the island, SE of Punta Suárez,
c. 500 m inland from coast, 1 22 0 5700 S, 89 43 0 800 W, alt.
2013
Arthoniales in the Galapagos—Bungartz et al.
749
Fig. 3. A–B, Cryptothecia striata; A, morphotype with ascigerous areas forming broad pustules, not arranged in striae
(Bungartz 3710); B, pyriform to subglobose ascus with a single, oblong, slightly bent, muriform ascospore (RivazPlata 4046). C–F, Helminthocarpon leprevostii, C, thallus aspect with short, broad, swollen lirellate ascomata
(Bungartz 4390); D, cross-section of a lirella, outside covered by a thick thallus layer, hamathecium of strongly
ramified and anastomosing paraphysoids, flanked by carbonized exciple (Aptroot 64570); E, two asci, the one on
the left with muriform ascospores, the one on the right closely entwined by strongly ramified and anastomosing
paraphysoids (Aptroot 64570); F, single ascospore (Aptroot 64570). Scales: A ¼ 3 mm; B ¼ 25 mm; C ¼ 10 mm;
D ¼ 02 mm; E & F ¼ 30 mm.
750
THE LICHENOLOGIST
133 m, dry zone, on bark, 2010, Bungartz 8972 (CDS
45790); trail from Bahı́a Manzanillo on the N-coast of
the island to the highest point, 1 22 0 1900 S, 89 42 0 700 W,
alt. 125 m, dry zone, on bark, 2010, Yánez 1686 (CDS
45569). Isla Floreana, Cerro Pajas, inside the crater,
1 17 0 4900 S, 90 27 0 2300 W, alt. 379 m, humid zone, on
bark, 2011, Bungartz 9285 (CDS 46511); trail from
Black Beach to highlands, on bark, 25 iv 1976, Weber s.
n. (COLO 294611, L-62910); trail going to Post Office
Bay off the dirt road between highlands and Puerto
Velasco Ibarra, cliff at NE-side of trail (mirador),
1 17 0 400 S, 90 26 0 3700 W, alt. 365 m, on bark, 2011,
Yánez 2113 (CDS 46578). Isla Pinta, along the trail up
to the summit from the S-coast, 0 34 0 900 N, 90 44 0 5900 W,
alt. 252 m, transition zone, on bark, 2007, Bungartz 5712
(CDS 33350); 0 34 0 500 N, 90 44 0 5700 W, alt. 237 m, transition zone, on bark, 2007, Bungartz 5905 (CDS 33582);
Bungartz 5922 (CDS 33599). Isla San Cristóbal, SW
foothills of Media Luna, inland from the NW-coast of
the island along the trail from Galapagera to Media
Luna; bottom of small crater to the NW of Media
Luna, 0 43 0 5300 S, 89 18 0 5700 W, alt. 124 m, dry zone,
on bark, 2007, Bungartz 6239 (CDS 34451). Isla Santa
Cruz, Bellavista, along road, 0 41 0 3500 S, 90 19 0 2700 W,
alt. 200 m, humid zone, on bark, 2005, Aptroot 63307
(CDS 30047); farm of Don Cabrera between Bellavista and Cascajo, c. 1 km W of Cascajo, 0 40 0 3900 S,
90 17 0 2100 W, alt. 249 m, humid zone, agricultural area,
on wood, 2010, Yánez 1491 (CDS 44921); on the North
side of the island, along the dirt road to the ash quarry
Mina Granillo Rojo, 0 36 0 5600 S, 90 22 0 300 W, alt. 570
m, transition zone, on bark, 2006, Aptroot 64570 (CDS
31142), Aptroot 64556 (CDS 31128). Isla Santiago,
along the trail from Bucanero to Jaboncillos, c. 1 km below
the summit, Cerro Gavilan, 0 11 0 4500 S, 90 47 0 2000 W,
alt. 680 m, transition zone, on bark, 2006, Aptroot
65449 (CDS 32037), Bungartz 4676 (CDS 28763); c. 7
km inland from the E-coast, e at the same latitude as
Bahı́a Sullivan, 0 17 0 400 S, 90 38 0 1800 W, alt. 192 m, dry
zone, on bark, 2006, Bungartz 5185 (CDS 29398). Isla
Isabela, Volcán Alcedo, along the trail going up the Eslope, at the NW-side of the trail, 0 24 0 200 S, 91 2 0 3600 W,
alt. 410 m, dry zone, on bark, 2006, Bungartz 4390
(CDS 28475); 0 24 0 300 S, 91 2 0 3500 W, alt. 434 m, dry
zone, on bark, 2006, Bungartz 4405 (CDS 28490), Aptroot 64953 (CDS 31532); 0 24 0 700 S, 91 2 0 5500 W, alt.
493 m, dry zone, on bark, 2006, Bungartz 4448 (CDS
28534); 2006, Aptroot 64965 (CDS 31545). Volcán
Sierra Negra, Las Merceditas, 0 51 0 5800 S, 91 0 0 5000 W,
alt. 208 m, humid zone, agricultural area, on bark, 2008,
Bungartz 8294 (CDS 40940).
Herpothallon Tobler
Flora, Jena 131: 446 (1937)
Herpothallon aff. confluenticum
Aptroot & Lücking
In Aptroot, Thor, Lücking, Elix & Chaves, Biblioth.
Lichenol. 99: 35 (2009)
Vol. 45
(Fig. 4A)
Thallus corticolous, delimited by a compact brown prothallus; thallus surface greyish
to greenish white, ecorticate, arachnoid to
cottony, in the centre densely covered by
pale beige to greyish white, loose, ‘fluffy’,
ecylindrical to sparsely branched pseudisidia, (20–)30–40(–60) mm diam.; medulla
white, lacking calcium oxalate crystals.
Asci and pycnidia not observed.
Spot tests and chemistry. P--, K--, C--, KC--;
UV-- (pale), ILugol’s--; confluentic and/or hyperconfluentic acid.
Distribution and ecology. Pantropical, new
to Galapagos and Ecuador; all specimens
collected in the humid zone, both on native
and introduced trees, rarely on rock, in
shaded and sheltered habitats.
Notes. How well the Galapagos material
corresponds to C. confluenticum s. str. remains uncertain. Unlike the description in
the protologue (Aptroot et al. 2009), the few
Galapagos specimens collected so far are all
small, very thin and have poorly developed
thalli. With storage in the herbarium their
pseudisidia develop a epale pinkish hue
similar to fresh pseudisidia of Syncesia leprobola, a species for which these specimens
were originally mistaken. Thalli of S. leprobola, however, do not contain confluentic
acid; instead they are characterized by protocetraric acid and thus react distinctly P+ red.
The Galapagos specimens have a conspicuous, relatively compact brown prothallus
that clearly delimits individual thalli. The
description of this species in Aptroot et al.
(2009) mentions a dirty whitish prothallus
and a whitish to brownish hypothallus, but
the photograph therein (fig. 3D) shows a
much better developed specimen, which also
appears to be delimited by a fine brown line.
Specimens examined. Ecuador: Galapagos: Isla
Floreana, Asilo de la Paz, Cerro Wittmer, road to Post
Office Bay, 1 18 0 412900 S, 90 27 0 42900 W, humid zone,
on bark, 2010, Hillmann GAL-81 (CDS 44907); Isla
Isabela, Volcán Alcedo, outer E-exposed slope just
below the crater rim, 0 25 0 1700 S, 91 5 0 800 W, alt. 1077 m,
humid zone, on rock, 2006, Aptroot, A. 65176 (CDS
31760); Isla Santa Cruz, lı́nea del Parque Nacional
2013
Arthoniales in the Galapagos—Bungartz et al.
751
Fig. 4. A, Herpothallon aff. confluenticum, small, thin, arachnoid thallus with few pseudisidia and a distinct brown
prothallus (Nugra 137). B–D, Herpothallon echinatum (Bungartz 5616); B, general thallus aspect of two merged thalli
delimited by a white, arachnoid prothallus; C, central thallus part with pycnidiate pseudisidia; D, close-up of pycnidiate pseudisidia; D, squash preparation of pycnidiate pseudisidium with aeruginose ostiole and bacilliform conidia.
E–F, Herpothallon granulare; E, radiating white fibrous prothallus strands along the thallus margin and granular
pseudisidia in the centre (Nugra 889); F, radiating fibrous thallus strands loosely covered in granular pseudisidia
(Bungartz 4997). Scales: A & C ¼ 1 mm; B ¼ 2 mm; D ¼ 15 mm; E & F ¼ 5 mm.
752
THE LICHENOLOGIST
Galápagos, sector el Camote a lindero de la finca de Don
René Valle, 0 38 0 211000 S, 90 17 0 572000 W, alt. 487 m,
zona húmeda, sobre corteza, 2006, Nugra 135 (CDS
32789); Nugra 137 (CDS 32791); along trail from Media Luna to El Puntudo, 0 39 0 98000 S, 90 19 0 592900 W,
alt. 724 m, humid zone, on bark, 2008, Clerc 08-114
(CDS 39968); Steve Divine Farm, near Tortoise Road,
off the main road to Baltra, at the entrance to lava tunnel,
Tortoise Territory, 0 39 0 5100 S, 90 24 0 1600 W, alt. 364 m,
humid zone, agricultural area, on rock, 2006, Bungartz
3966 (CDS 27848).
Vol. 45
root 64330 (CDS 30895); along the trail down into the
crater at Camote, 0 38 0 1700 S, 90 17 0 4200 W, alt. 418 m,
humid zone, on bark, 2007, Bungartz 5616 (CDS
33241).
Herpothallon granulare (Sipman)
Aptroot & Lücking
In Aptroot, Thor, Lücking, Elix & Chaves, Biblioth.
Lichenol. 99: 43 (2009)
(Figs. 4E–F)
Herpothallon echinatum Aptroot et al.
In Aptroot, Thor, Lücking, Elix & Chaves, Biblioth.
Lichenol. 99: 38 (2009)
(Figs. 4B–D)
Thallus corticolous, delimited by a distinct
byssoid prothallus of white, radiating hyphae;
thallus surface greyish to greenish white, ecorticate, cottony, in parts almost granular, in the
centre densely covered by slightly darker,
compact globular to ecylindrical pseudisidia containing a central pycnidium; medulla
white, filled with colourless to pale brownish
granules and sparse calcium oxalate crystals
(insoluble in KOH, forming colourless,
needle-shaped crystals in 25% H2SO4).
Asci not observed.
Pycnidia embedded within pseudisidia,
opening with an aeruginose ostiole, pigmentation occasionally eextending along
the pycnidial wall, conidiophores simple,
with oblong to bacilliform conidia, 3–4 1–
15 mm.
Spot tests and chemistry. P+ deep golden
yellow, K--, C--, KC--, UV-- (dull), ILugol’s--;
psoromic acid.
Distribution and ecology. Pantropical, new
to Galapagos and Ecuador; the few known
specimens were collected in the agricultural
areas of the humid zone of Santa Cruz, all
growing on introduced trees and shrubs (Cedrela odorata, Cestrum auriculatum), in shaded
and sheltered habitats.
Specimens examined. Ecuador: Galapagos: Isla Santa
Cruz, abandoned farm along the northern part of the
loop road from Bellavista to Garrapatero, 0 40 0 5800 S,
90 18 0 3100 W, alt. 255 m, humid zone, agricultural area,
on bark, 2006, Aptroot 64328 (CDS 30893); 2006, Apt-
Thallus corticolous, rarely foliicolous, delimited by a broad prothallus of loosely radiating fibrous strands of white hyphae; thallus
surface greenish grey to dull green, with storage becoming paler, ecorticate, entire thallus
of thick fibrous strands of loosely interwoven
hyphae, abundantly covered in coarsely granular pseudisisdia; medulla poorly differentiated, indistinct, with hyphae covered by
colourless to pale brownish granules, lacking
calcium oxalate crystals (not forming colourless, needle-shaped crystals in 25% H2SO4).
Asci and pycnidia not observed.
Spot tests and chemistry. P--, K--, C--, KC--;
UV--, ILugol’s--; perlatolic acid.
Distribution and ecology. Pantropical, new
to Galapagos and Ecuador; one of the most
common species from the upper transition
zone through the humid zone into high-altitude transition zone; on a wide variety of both
native and introduced trees; typically in semishaded to shaded and esheltered habitats.
Selected specimens examined. Ecuador: Galapagos: Isla
Floreana, Asilo de la Paz, Cerro Wittmer, road to Post
Office Bay, 1 18 0 412900 S, 90 27 0 42900 W, alt. 0 m,
humid zone, on bark, 2010, Hillmann GAL-83 (CDS
44909); Cerro Pajas, inside the crater, 1 17 0 492900 S,
90 27 0 2300 W, alt. 379 m, humid zone, on bark, 2011,
Bungartz 9260 (CDS 46486); on W-slope of Cerro
Alieri, permanent plot 2, 1 17 0 241000 S, 90 27 0 89000 W,
alt. 347 m, transition zone, on bark, 2011, Bungartz
9312 (CDS 46538); on W-slope of Cerro Alieri,
1 17 0 241000 S, 90 27 0 89400 W, alt. 347 m, transition
zone, on bark, 2011, Bungartz 9333 (CDS 46620). Isla
Santa Cruz, on farm N of Bellavista, 0 41 0 400 S,
90 19 0 2900 W, alt. 250 m, humid zone, agricultural area,
on bark, 2005, Aptroot 63314 (CDS 30054); temporary
Cinchona weather station, along the trail to El Puntudo,
0 39 0 65900 S, 90 19 0 573900 W, alt. 698 m, humid zone,
on bark, 2005, Bungartz 3283 (CDS 26925); above
the Finca of Galo Torres, inside the Park boundary,
2013
Arthoniales in the Galapagos—Bungartz et al.
0 37 0 4500 S, 90 24 0 100 W, alt. 536 m, humid zone, on
bark, 2006, Bungartz 4997 (CDS 29210). Isla Isabela,
Volcán Alcedo, outer SE-exposed slope, ca. 25 km below the crater rim, 0 26 0 1300 S, 91 4 0 3300 W, alt. 785 m,
transition zone, on bark, 2006, Bungartz 4238 (CDS
28308).
Herpothallon hyposticticum Bungartz &
Elix sp. nov.
MycoBank no. 804739
Herpothallon with thin to moderately thickened dense,
contiguous to rimose thallus, granular to cylindrical,
sparsely branched pseudisidia, containing hypostictic
and hyposalazinic acid.
Type: Ecuador, Galapagos Islands, Isla Santa Cruz,
near the CDRS field-weather station below the summit
of Cerro Crocker, 0 38 0 3500 S, 90 19 0 4200 W, humid
zone, much overgrown with dead Cinchona pubescens
trees (killed by invasive species programme), N-exposed
slope, on trunks and twigs of dead Cinchona pubescens, 28
xii 2005, Bungartz 3306 (cds 26961—holotype).
(Fig. 5A–F)
Thallus corticolous, rarely saxicolous, delimited by an inconspicuous, dense prothallus of white hyphae; thallus surface greyish
white, with storage ebeige to pale yellowish,
ecorticate, thin and arachnoid to moderately
thickened and then developing a dense, compact, contiguous to sparsely fissured or even
distinctly rimose surface, sparsely to abundantly covered by minutely granular, powdery
to distinctly cylindrical pseudisidia, 50–80
mm diam., fairly uniform in size; medulla
white, filled with few, sparse calcium oxalate
crystals (insoluble in KOH, forming colourless, needle-shaped crystals in 25% H2SO4).
Asci and pycnidia not observed.
Spot tests and chemistry. P--, K+ yellow,
KC--, C--; UV+ bright yellow; medulla
ILugol’s+ weakly blue in parts; hypostictic
and hyposalazinic acid.
Distribution and ecology. Currently known
only from Galapagos; occurring from the
upper transition to the humid zone, on native
or endemic (Pisonia floribunda, Zanthoxylon
fagara, Scalesia pedunculata) and introduced
tree species (Cinchona pubescens, Persea americana), in semi-shaded to shaded, esheltered
habitats; one specimen from a shaded rock
overhang.
753
Notes. Herpothallon hyposticticum is unusual
in several aspects. Specimens consistently
contain hypostictic and hyposalazinic acid,
substances that are usually accessories of
stictic acid and norstictic acid, yet these principal metabolites are absent from the thalli.
The species is morphologically unusually
variable. Some thalli are abundantly covered
in well-developed pseudisidia, whereas the
thallus itself is poorly developed, essentially
present only as a thin layer of arachnoid hyphae. In other specimens the thallus is better
developed, cottony to dense, ecompacted
and then sparsely fissured. These specimens
generally have pseudisidia that are much less
developed, in some parts of the thallus they
remain in fact granular and thus look like
granular soredia. In other parts of the same
thallus these granules nevertheless become
cylindrical and even sparsely branched, indicating that they are all precursors of true
pseudisidia. Finally some specimens have an
unusually well-developed, sometimes almost
rimose thallus and generally only develop
granular pseudisidia. All specimens have a
weakly I+ blue medulla, at least in part.
Specimens with well-developed thalli and
poorly developed pseudisidia are superficially
similar to species of Cryptothecia, but when
elevated regions of the thallus, thought to
perhaps correspond to ascigerous areas, were
sectioned, no asci or acospores could be
found.
Specimens examined: Ecuador: Galapagos: Isla Isabela,
Volcán Alcedo, on top of the crater rim, 0 27 0 3300 S,
91 6 0 4900 W, alt. 1051 m, humid zone, on bark, 2006,
Bungartz 4105 (CDS 28073); Isla Santa Cruz, off
the dirt road to Mina Granillo Rojo, on the N-side of the
island, 0 37 0 200 S, 90 22 0 600 W, alt. 294 m, transition zone,
on bark, 2006, Bungartz 4972 A (CDS 44662); along
the road from Bellavista to Los Gemelos, 0 38 0 1000 S,
90 23 0 4500 W, alt. 579 m, humid zone, on bark, 2006,
Bungartz 3489 (CDS 27245); lı́nea del Parque Nacional Galápagos, cerca de la finca de Galo Torres,
0 37 0 457700 S, 90 24 0 000 W, alt. 570 m, zona húmeda, sobre corteza, 2006, Nugra 13 A (CDS 38749), Nugra 20 B
(CDS 54431); Isla Santiago, summit of Cerro Gavilan, inner N- and NE-exposed crater rim, 0 12 0 2000 S,
90 47 0 300 W, alt. 840 m, humid zone, on rock, 2006, Aptroot 65713 (CDS 32305).
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THE LICHENOLOGIST
Vol. 45
Fig. 5. A–E, Herpothallon hyposticticum (corticolous specimens); A–B, thin arachnoid thallus with dense, abundant
and well-developed pseudisidia (Bungartz 3489); A, thallus centre; B, thallus margin; C, thin arachnoid thallus with
few, scattered, well-developed pseudisidia (Nugra 13 A); D, well-developed compact thallus with few, immature,
granular pseudisidia (Bungartz 4972 A); E, well-developed, compact to rimose thallus with scattered, well-developed
pseudisidia (holotype,); F, Herpothallon hyposticticum (saxicolous specimen), well developed, compact to rimose thallus
with immature, granular pseudisidia (Aptroot 65713). Scales: A & D ¼ 2 mm; B, E & F ¼ 15 mm; C ¼ 1 mm.
2013
Arthoniales in the Galapagos—Bungartz et al.
Herpothallon rubrocinctum (Ehrenb.)
Aptroot et al.
In Aptroot, Thor, Lücking, Elix & Chaves, Biblioth
Lichenol. 99: 61 (2009)
(Fig. 6A–B)
Thallus corticolous, rarely saxicolous or
foliicolous, delimited by a distinct byssoid
prothallus of carmine red, radiating hyphae;
thallus surface greyish to greenish white,
ecorticate, dense, cottony, felt-like, in the
centre covered by carmine red, granular
pseudisidia; medulla white, in parts filled
with carmine red granules and sparse calcium oxalate crystals (insoluble in KOH,
forming colourless, needle-shaped crystals
in 25% H2SO4).
Asci and pycnidia not observed.
Spot tests and chemistry. P--, K-- (green thallus areas), K+ purple (carmine red prothallus & pseudoisidia), C--, KC--; UV-- (dull),
ILugol’s--; confluentic, chiodectonic, 2’-Omethylmicrophyllinic acids.
Distribution and ecology. Cosmopolitan; by
far the most common Herpothallon species in
Galapagos, often very abundant and sometimes forming thalli of several decimeters
across; common in the humid and upper
transition zone, rarely also in the dry zone,
on a wide variety of both native and introduced tree species, rarely also on rock; both
in sunny, exposed and eshaded and sheltered habitats.
Selected specimens examined. Ecuador: Galapagos: Isla
Fernandina, W-side, alt. 335 m, transition zone,
Cavagnaro, D. s. n. (COLO 193387, L-40446). Isla
Floreana, c. 1 km S of Cerro Verde, 1 18 0 3400 S,
90 25 0 1900 W, alt. 299 m, transition zone, on bark,
2011, Yánez 1945 (CDS 48297); trail going to Post
Office Bay off the dirt road between highlands and
Puerto Velasco Ibarra, 1 17 0 1700 S, 90 26 0 3500 W, alt.
360 m, transition zone, on bark, 2011, Yánez 1921
(CDS 48275). Isla Pinta, along the trail up to the summit from the S-coast, 0 34 0 3900 N, 90 45 0 700 W, alt. 436
m, humid zone, on bark, 2007, Bungartz 5828 (CDS
33501); 0 34 0 3400 N, 90 45 0 800 W, alt. 414 m, transition
zone, on bark, 2007, Bungartz 5735 (CDS 33378);
northern part of the western cliff above Las Pampas,
0 35 0 1100 N, 90 46 0 3400 W, alt. 356 m, dry zone, on bark,
2008, Nugra 606 (CDS 38984). Isla Pinzón, summit,
transition zone, on bark, Cavagnaro, D. s. n. (COLO
192819, L-40489). Isla San Cristóbal, Bailey s. n.
755
(COLO 151174, S-27084); along trail between entrance
to Cerro Pelado and Cerro Partido, trail to El Ripioso,
0 51 0 4000 S, 89 27 0 3800 W, alt. 383 m, transition zone, on
bark, 2007, Bungartz 6678 (CDS 34914); along trail to
Ochoa, along the northern border of the National Park,
0 53 0 3900 S, 89 33 0 1900 W, alt. 315 m, humid zone, on
bark, 2008, Herrera-Campos 477 (CDS 43368). Isla
Santa Cruz, above the Finca of Galo Torres, inside the
Park boundary, 0 37 0 4500 S, 90 24 0 100 W, alt. 536 m,
humid zone, on bark, 2006, Bungartz 4993 (CDS
29206); along the dirt road from Bellavista to Media
Luna, farmland on the W-side of the road, 0 40 0 5400 S,
90 19 0 2600 W, alt. 285 m, humid zone, agricultural area,
on bark, 2006, Bungartz 3989 (CDS 27919); along
the road from Bellavista to Los Gemelos, 0 38 0 1000 S,
90 23 0 4500 W, alt. 579 m, humid zone, on bark, 2006,
Bungartz 3476 (CDS 27232), Bungartz 3493 (CDS
27249), Aptroot 63866 (CDS 30424). Isla Santiago, E
of salt lake at Santiago Bay, 0 14 0 2500 S, 90 48 0 5000 W,
alt. 170 m, dry zone, on bark, 1971, Pike 2707 (COLO
263253, L-56175), Pike 2707 (OSC 54834); summit of
Cerro Gavilan, inner N- and NE-exposed crater rim,
0 12 0 2000 S, 90 47 0 300 W, alt. 840 m, humid zone, on
rock, 2006, Aptroot 65753 (CDS 32345); on bark, 03Jan-1906, Stewart 3446 (FH 197182). Isla Isabela,
Volcán Alcedo, outer SE-exposed slope and crater
rim, 0 27 0 2900 S, 91 7 0 1900 W, alt. 1089 m, humid zone,
on bark, 2006, Bungartz 4041 (CDS 27971), Bungartz
4241 (CDS 44660); lower crater slopes above the first
Caseta del Parque; below the cloud layer, 0 58 0 3700 S,
91 25 0 2600 W, alt. 456 m, humid zone, on bark, 2012,
Nugra 1036 (CDS 52200); Volcán Sierra Negra, along
dirt road from Puerto Villamil to crater of Sierra Negra,
farmland, 0 50 0 3800 S, 91 3 0 5200 W, alt. 564 m, humid
zone, on bark, 2007, Bungartz 6891 (CDS 36379);
Cueva del Sucre, at the entrance (parking), SE side of
the island, 0 50 0 3300 S, 91 1 0 3700 W, alt. 369 m, humid
zone, on bark, 2008, Herrera-Campos 10644 (CDS 40381).
Herpothallon rubroechinatum Frisch &
G. Thor
In A. Frisch, G. Thor and J. A. Elix, Bryologist 113: 145
(2010)
(Figs. 6C–D)
Thallus corticolous, delimited by a distinct
byssoid prothallus of white, radiating hyphae
typically at least in part with a rust-red
pigment; thallus surface greyish white, with
storage becoming ebeige, ecorticate, cottony,
in parts almost granular, in the centre densely
covered by slightly darker, compact pseudisidia that appear as if sprinkled with rust-red
crystals; pseudisidia dimorphic, either swollen, globular containing one central pycnidium or, with age, elongate cylindrical to
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THE LICHENOLOGIST
Vol. 45
Fig. 6. A & B, Herpothallon rubrocinctum; A, close-up of thallus with striped carmine red prothallus, greenish thallus
and carmine red pseudisidia (Ertz 11551); B, close up of dense, compact carmine red prothallus (Aptroot 63132).
C & D, Herpothallon rubroechinatum (Bungartz 3488); C, thallus with central pseudisidia, delimited by rust red prothallus; D, cylindrical pseudisidia with rust red pigment crystals. E & F, Herpothallon saxorum; E, dense, compacted
fibrous prothallus strands covered with coarse granular pseudisidia (Bungartz 4874); F, loose, arachnoid prothallus
strands along the thallus margin covered towards the centre in coarse granular pseudisidia (Bungartz 7793). Scales:
A ¼ 10 mm; B ¼ 5 mm; C ¼ 4 mm; D ¼ 1 mm; E & F ¼ 2 mm.
2013
Arthoniales in the Galapagos—Bungartz et al.
evermiform; medulla white, filled with brownish granules and sparse calcium oxalate crystals (insoluble in KOH, forming colourless,
needle-shaped crystals in 25% H2SO4).
Asci not observed
Pycnidia embedded within pseudisidia,
opening with an aeruginose ostiole, pigmentation occasionally extending along the pycnidial wall, conidiophores simple, with oblong to bacilliform conidia, 3–4 1–15 mm.
Spot tests and chemistry. P+ bright yellow;
K-- (greenish white thallus areas), K+ purple
(bright red crystal of the pseudoisidia and
rust reddish prothalline hyphae), C--, KC--,
UV-- (dull), ILugol’s--; psoromic acid.
Distribution and ecology. Neotropical, new
to Galapagos and Ecuador; the few known
specimens were collected in the humid zone
of Santa Cruz, growing on both the endemic
tree Scalesia pedunculata and the introduced
trees Cinchona pubescens and Cedrela odorata,
in semi-shaded to shaded and esheltered
habitats.
Selected specimens examined: Ecuador: Galapagos: Isla
Santa Cruz, along the road from Bellavista to Los
Gemelos, 0 38 0 1000 S, 90 23 0 4500 W, alt. 579 m, humid
zone, on bark, 2006, Bungartz 3488 (CDS 27244), Aptroot 63826 (CDS 30384); along the road to Baltra, S
of Los Gemelos, 0 38 0 4400 S, 90 20 0 500 W, alt. 741 m, humid zone, on bark, 2006, Bungartz 5511 (CDS 36156);
lı́nea del Parque Nacional Galapagos, cerca de la finca
de Galo Torres, 0 37 0 4600 S, 90 24 0 000 W, alt. 570 m,
zona húmeda, sobre corteza, 2006, Nugra 17 (CDS
32670), Nugra 19 (CDS 32672); off the dirt road to
Mina Granillo Rojo, on the N-side of the island,
0 37 0 200 S, 90 22 0 600 W, alt. 294 m, transition zone, on
bark, 2006, Bungartz 4972 B (CDS 29185).
Herpothallon saxorum Bungartz & Elix
sp. nov.
MycoBank no. 804740
Similar to Herpothallon granulare, but containing confluentic acid, brialmontin 1 and brialmontin 2 instead
of perlatolic acid; saxicolous.
Type: Ecuador, Galapagos Islands, Isla Santiago,
along the trail from the caseta in La Central to La
Bomba (at the coast), cliff c. 25 km NE of the caseta,
0 13 0 4100 S, 90 44 0 1000 W, alt. 533 m, transition zone,
SW-exposed basalt cliff with some ferns (Adiantum concinnum, Pityrograma calomelanos var. calomelanos, and
Blechnum polypodioides) growing in crevices, on horizontal ledges of SW-exposed front of basalt cliff; semishaded, wind- and rain-exposed, 25 iii 2008, Bungartz
4874 (cds 29073—holotype).
757
(Figs. 6E–F)
Thallus saxicolous, delimited by a broad
prothallus of white arachnoid hyphae that
soon aggregate into loosely radiating fibrous
strands; thallus surface dull greenish to beige,
with storage becoming ewhite, ecorticate,
entire thallus initially of loosely interwoven
hyphae that soon form distinct fibrous strand
and with age rarely become more densely interwoven and almost cottony, typically not
developing into a distinctly differentiated
thallus, but instead covered with abundant,
coarsely granular pseudisidia; medulla poorly
differentiated, indistinct, with hyphae covered by colourless to pale brownish granules,
lacking calcium oxalate crystals (not forming
colourless, needle-shaped crystals in 25%
H2SO4).
Asci and pycnidia not observed.
Spot tests and chemistry. P+ yellow, K+
yellow, C--, KC--; UV+ (dull), medulla
ILugol’s--; brialmontin 1, brialmontin 2, confluentic acid.
Distribution and ecology. Currently known
only from Galapagos and very likely endemic;
moderately common throughout the dry and
lower transition zone, rarely extending into
the humid zone; on sheltered, shaded rock
faces and overhangs.
Notes. Morphologically this new species
closely resembles H. granulare, but it is
chemically distinct and is not corticolous,
instead growing on sheltered rock surfaces.
When the material was first analyzed in solvent C, specimens were believed to represent
a K+ yellow chemotype of H. granulare that
contained atranorin in addition to perlatolic
acid. However, in solvent A, the specimens
were shown to contain confluentic acid and
brialmontin 1 and brialmontin 2 (the latter
two substances have almost identical R f to
atranorin in solvent C).
The only other species of Herpothallon
known to contain related substances (brialmontic acid, methylbrialmontic acid, and dimethylbrialmontic acid) is H. brialmonticum
Aptroot & Elix. This corticolous species is
morphologically dissimilar from H. granulare,
forming a much more compact, thick, cottony thallus with smoother, globose pseudisidia. In contrast, H. saxorum is composed of
758
THE LICHENOLOGIST
fibrous hyphal strands with rather coarse,
distinctly granular pseudisidia.
Specimens examined. Ecuador: Galapagos: Isla Santa
Cruz, along the road from Bellavista to Los Gemelos,
0 38 0 1000 S, 90 23 0 4500 W, alt. 579 m, humid zone, open
Scalesia pedunculata forest with Rubus niveus, Psychotria
rufipes, Zanthoxylum fagara, and scattered lava boulders,
on rock of top and slope of basalt boulders; semi-shaded,
wind- and rain-sheltered, 2006, Bungartz 3470 B (CDS
27226); above Mina Granillo Rojo on the N-side of the
island, 0 37 0 900 S, 90 21 0 577300 W, alt. 619 m, transition
zone, upper transition zone; open forest of Scalesia
pedunculata, Psidium galapageium, Zanthoxylum fagara
and few Pisonia floribunda with basaltic rock outcrops,
on rock of N-exposed overhang of basalt boulder; semishaded, wind- and rain-sheltered, 2008, Bungartz 8111
(CDS 40757); Isla Isabela, Volcán Darwin, Isabela,
south-western slope, above Tagus Cove, 0 13 0 281900 S,
91 19 0 178900 W, alt. 872 m, transition zone, top of lava
flow in open scrubland of Croton scouleri and Dodonaea
viscosa with Cordia revoluta and some Opuntia insularis,
on rock of S-exposed overhang of basalt boulder;
shaded, wind- and rain-sheltered, 2007, Bungartz 7740
(CDS 38246); south-western slope, above Tagus Cove,
0 13 0 5900 S, 91 20 0 800 W, alt. 597 m, dry zone, open Bursera graveolens forest with Croton scouleri and Macraea
laricifolia shrubs, few Chiococca alba and Scalesia microcephala, among lava boulders and outcrops, on rock of
SW-exposed overhang of basalt boulder; shaded, windand rain-shaded, 2007, Bungartz 7803 (CDS 38312);
Bungartz 7793 (CDS 38302); Volcán Sierra Negra,
Muro de las Lagrimas W of Puerto Villamil, along
the stairs going up behind the wall, 0 57 0 527300 S,
91 0 0 467900 W, alt. 78 m, dry zone, dry zone vegetation
with Bursera graveolens and Opuntia echios, slope 45 N,
on rock of basaltic rocks at the side of the stairs, 2008,
Herrera-Campos, M.A. 10745, (CDS 40483).
Discussion
With nine different species of Herpothallon
and Cryptothecia now reported from the
Galapagos, four of them new to science, this
group of species at first appears more diverse
than anticipated, yet the number of species
found in the archipelago is comparable to
other genera with similar ecology and distribution mode. Herpothallon for example generally inhabits humid, sheltered habitats and
reproduces almost exclusively by vegetative
propagules rather than ascospores. This ecology and means of reproduction is little different from Lepraria and the numbers of species
in both genera are almost the same: six species of Herpothallon are now reported from
Galapagos and a recent treatment of Lepraria
(submitted for publication) lists five species.
Vol. 45
It is difficult to assess whether the new species described here are endemic to the archipelago. The Galapagos are of course famous
as a ‘laboratory of evolution’ characterized
by their high degree of endemism, a result
of their geographic isolation in combination
with climatic and geomorphological variation and thus habitat diversity. Due to the
relatively late arrival of humans much of the
original biodiversity is considered to still be
present in the islands (Snell et al. 2002).
Isolation and thus effectiveness of dispersal units are clearly the limiting factors
for any species to reach the archipelago and
lichens, characterized by microscopic propagules clearly have an advantage in crossing
the 1,000 km distance from the mainland
compared with many other organisms with
much heavier propagules. Lichen species
that reproduce and disperse mainly by ascospores may be hindered in establishing a
successful symbiosis if they fail to encounter
suitable photobionts, but propagules like soredia, granules, isidia, and thallus fragments in
general, will almost always be larger and
heavier. Thus it is not clear which species,
those that reproduce sexually or those that
mainly reproduce asexually, have an evolutionary advantage in reaching the islands
and evolving into new, endemic species.
Studies which try to compare rates of
endemism across different species groups
are challenging when species groups being
assessed are generally not well studied. It
is quite possible that the species newly described here are endemic to the Galapagos,
but since this group of species is very poorly
known, it is difficult to assess whether or not
these species may also be present on the
South American continent.
Two of the three genera studied here are
unusual insofar that their asci do not develop
in distinct ascomata, but instead within ascigerous areas (Cryptothecia) or solitary inside
pseudisidia (Herpothallon fertile, not observed
in Galapagos, see Aptroot et al. 2009).
Among lichenized fungi this ascus ontogeny,
the non-aggregation of hamathecial filaments
within supporting ascomatal structures, is
only known from one other genus, Stirtonia,
which differs in having transversely septate,
rather than muriform spores. In Cryptothecia
2013
Arthoniales in the Galapagos—Bungartz et al.
the ascigerous areas may barely differ from the
surrounding thallus and these species almost
appear as one smooth, ‘sterile’ crust. More
typically, however, the ascigerous areas are
somewhat confined and delimited as warts or
pustules, sometimes resembling irregular
patches of ascomata in Arthonia or Arthothelium, both genera of the Arthoniaceae with distinct interascal filaments aggregating into a
well-developed hamathecium (Wolseley &
Aptroot 2009).
Both Cryptothecia and Herpothallon have a
cottony-byssoid thallus. As they lack a cortex the hyphae are usually not very dense or
compacted and in some species, like Herpothallon granulare, they are in fact very loosely
bundled into fibrous strands bearing granular packets of pseudisidia. This growth form
could be called leprose (sensu Lendemer
2011), because the main thallus of the species is actually formed by the granules and
a thallus with a distinct photobiont layer and
medulla does not develop. Only one other
species of Herpothallon, the newly described
H. saxorum, has a similarly leprose growth
form, even though the hyphae of this species
tend to become more densely interwoven
and then quite similar to the cottony-byssoid
thallus typically observed in Herpothallon or
Cryptothecia.
Clearly the cottony-byssoid growth form
of sterile thalli alone is not a good indication
of how these species are related. Frisch &
Thor (2010) recently described the genus
Crypthonia to accommodate species with
byssoid thalli like those of Herpothallon and
Cryptothecia, but with 1–3 septate ascospores
similar to those of Arthonia. Ascomata of
Crypthonia are less distinct than those of
Arthonia, and some species of Cryptothecia
show at least a tendency of their asci to be
closely aggregated, almost forming inside
structures similar to ascomata (see the descriptions of C. darwiniana and C. galapagoana).
When fertile specimens are absent it can
be difficult not only to assign a species to a
particular genus, but even the placement
within Arthoniales or Arthoniomycetes may
be in doubt. Nelsen et al. (2012) ‘‘dismantling Herpothallon’’ demonstrated that at least
759
one species previously included was in fact a
Diorygma lacking apothecia. Nelsen et al.
(2010) described the new genus Heiomasia,
with a species that forms thalli analogous to
those of Herpothallon, but like Diorygma is a
member of the Graphidaceae and thus part of
Lecanoromycetes rather than Arthoniomycetes.
Among the species treated here, the newly
described H. hyposticticum forms thalli that
appear superficially very similar to those of
Heiomasia (Fig. 5C shows a thallus similar
to that of H. sipmanii, though it lacks the discoid pseudisidia). As for all other species of
Herpothallon, accurate phylogenetic placement will ultimately have to wait until fertile
material is found and/or material is examined
with molecular tools.
With the medulla of H. hyposticticum reacting I+ blue at least in part, at first we believed
it could be described as Cryptothecia, but no
asci or ascospores were found and the presence of well-developed pseudisidia, at least
in some material (Fig. 5A & C), suggests
the species is currently best included in
Herpothallon. The distinction between Cryptothecia and Herpothallon no longer appears
to be as clear cut as outlined by Aptroot et
al. (2009). Nelsen et al. (2009, 2012) demonstrated that H. rubrocinctum appears to be
nested within Cryptothecia and Ertz & Tehler
(2011) stated in their revision of Arthoniales
that the genus Cryptothecia remains paraphyletic.
The morphological studies presented here
also suggest that the distinction between the
genera requires further study. Cryptothecia
galapagoana is abundantly fertile with well
defined ascigerous pustules, but its thalli are
also densely covered in granules that are
morphologically similar, if not identical, to
the granular pseudisidia of H. granulare or
H. saxorum, and H. hyposticticum shows a
transition from granular to cylindrical pseudisidia. Thus, some species of Cryptothecia
do not reproduce exclusively from ascospores,
but also form pseudoisidiate granules.
Most thalli of the species studied here are
thick and cottony-byssoid, but a few have an
unusually compact surface. It is possible that
the loose, cottony surface is an adaptation
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THE LICHENOLOGIST
to the high humidity of habitats where these
lichens typically grow. Their thalli are water
repellent and in humid habitats gas exchange
will be much enhanced, if lichen hyphae are
not closely compacted. Cryptothecia darwiniana on the other hand, though also ecorticate, always forms very dense thalli and is
regularly found in exposed habitats, on trees
of the dry Galapagos lowlands. Its pale yellowish crust contains high concentrations of
xanthones, possibly an adaptation to high
solar radiation present in these habitats.
This species is also unusual in other respects. Its ascigerous areas are initially broad,
flattened, irregular and only slightly elevated,
and when young barely distinguished from
the surrounding surface. With age they become more distinctly pustulate and sometimes even strongly convex. These pustules
may erode and may then even appear somewhat blackened due to increasing carbonization within. Although no distinct interascal
filaments can be differentiated from surrounding thalline hyphae, the asci within these ascigerous pustules seem to aggregate loosely
within ‘locules’ around which the carbonization develops. These ‘locules’ may even
merge and become aggregated in ramified
lines and, when eroded, then have some similarity to ascomata of a Syncesia. A second
species occasionally shows similar carbonization, though not as strong. Cryptothecia galapagoana has asci located in ascigerous areas
that contain a brownish pigment, which with
age accumulates and forms ‘locules’, though,
unlike in C. darwiniana, these ‘locules’ do
not aggregate in lines and they never resemble ramified lirellae.
Overall the ascigerous warts of both Cryptothecia darwiniana and C. galapagoana display tendencies towards some kind of ascomata formation and develop structures that
are surprisingly similar to the broad, beanshaped, short lirellae of Helminthocarpon leprevostii, a species that is occasionally found
in the same habitats as Cryptothecia darwiniana. Asci of H. leprevostii develop within a
distinct hamathecium of very strongly ramified and repeatedly anastomized paraphysoids. These paraphysoids form a dense layer
of hyphae enveloping individual asci, a phe-
Vol. 45
nomenon also similar to the paraphysoids of
Cryptothecia, where these hyphae are less
dense, but also closely surrounding each
ascus.
In Helminthocarpon the carbonized flanks
of the exciple are buried deep below a thick
thalline layer. This resembles the carbonized
‘locules’ of Cryptothecia darwiniana and C.
galapagoana, buried deeply within their ascigerous warts. Further, the large, thin-walled
muriform spores with curved septa are also
similar and both genera have ascospores that
react KI--.
Despite these similarities, Helminthocarpon
differs not only because of its better developed ascomata forming a true hamathecium
embraced by a distinct exciple, but also because it has a corticate thallus with an I-medulla.
Staiger (2002) in her monograph of Graphidaceae excluded Helminthocarpon referring to
Aptroot (1999, in Eriksson, Outline of Ascomycota, note 2603), who suggested including this
enigmatic lichen within Arthoniaceae and cites
Awasthi & Joshi (1979) as the first to discuss
its close similarity to some Cryptothecia species. Aptroot (1999) suggested that ascigerous
areas of Cryptothecia might ultimately develop
structures quite similar to those of Helminthocarpon (Aptroot 1999): ‘‘In fact, Helminthocarpon may represent the most developed
Cryptothecia-like ascomata.’’ Interestingly, the
most recent Outline of Ascomycetes (Lumbsch
& Hunhdorf 2010) no longer lists Helminthocarpon.
The lirellae of Helminthocarpon, though superficially similar to those of some species of
Graphis, anatomically resemble more closely
those of Opegrapha and related genera where
the exciple carbonization is typically coarser
and the asci often more loosely embraced
within a less well developed hamathecium.
The asci of Helminthocarpon are very similar
if not identical to those of Opegrapha: they
are two-layered, very thick-walled, with an
outer layer not staining in KI and an inner
layer KI+ pale blue, with no conspicuous
ocular chamber or tholus, though apically
thickening and typically tipped with a minute,
KI+ deep blue apical ring. Like Cryptothecia
and Opegrapha, asci of Helminthocarpon open
2013
Arthoniales in the Galapagos—Bungartz et al.
by bitunicate-fissitunicate dehiscence. Asci of
Cryptothecia have a similarly thickened bitunicate wall, but they are KI-- throughout, are
generally much broader and typically have a
distinctly thickened tholus with a small ocular
chamber.
Although species of Opegrapha s. str. do
not have muriform spores, those of the
closely related Dictyographa do; this genus
was originally reduced to synonymy (Ertz &
Diederich 2007), but recently resurrected
(Ertz & Tehler 2011). Most other genera
with muriform spores are now, however,
included in Arthoniaceae (Ertz & Tehler
2011) and, as discussed above, with their
curved septa the spores of Helminthocarpon
closely resemble those of Cryptothecia.
The phylogeny and consequent taxonomy
of Arthoniomycetes has changed considerably in recent years (Nelsen et al. 2009, Ertz
& Tehler 2011). Classical characters are
currently insufficient to come to a definite
conclusion at to whether the enigmatic Helminthocarpon be better placed in the Opegraphaceae or in the Arthoniaceae. Molecular
studies will also be necessary to re-assess the
significance of classical morphological and
anatomical characters in Cryptothecia and
Herpothallon.
We thank Frauke Ziemmeck for managing the cryptogam collection at CDS, helping with collecting, data
entry and curation of specimens. Successive Directors
of Science at the Charles Darwin Foundation have
supported this project: Alan Tye, Mark Gardener, and
Rodolfo Martinez. We are further indebted to the Galapagos National Park, especially its technical director
Washington Tapia for support and specimen export
permits. The Census of Galapagos Biodiversity and
the CDF Checklist of Galapagos Species is supported
by several grants to the Charles Darwin Foundation
(donors cited at http://www.darwinfoundation.org/
datazone/checklists/). A checklist of Galapagos lichens
is regularly updated and available at http://www.
darwinfoundation.org/datazone/checklists/lichens, where
contributing scientists are acknowledged. The lichen
inventory continues to receive funds from The Paul and
Bay Foundations and the Erwin Warth Stiftung. In 2010
an international lichen workshop was held in Galapagos,
supported by two National Science Foundation (NSF)
projects (DEB 0715660 to The Field Museum; PI
Robert Lücking; and DEB 0841405 to George Mason
University; PI James Lawrey, subcontract to the Charles
Darwin Foundation, local coordinator F. Bungartz).
During this workshop R. Lücking reviewed many specimens, also drawing attention to misidentifications of
Cryptothecia striata as Herpothallon philippinum. HPTLC
761
was largely carried out by the authors, but several people
also contributed much to some of these chemical analyses,
in particular Andrea Cahuana, Harald Jonitz, Georg
Hillmann and Alba Yánez. This publication is contribution number 2070 of the Charles Darwin Foundation for
the Galapagos Islands.
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