Egyptian Journal of Basic and Applied Sciences ISSN: (Print) 2314-808X (Online) Journal homepage: https://www.tandfonline.com/loi/teba20 Efficacy of silver nanoparticles mediated by Jania rubens and Sargassum dentifolium macroalgae; Characterization and biomedical applications Hani Saber, Eman A. Alwaleed, K.A. Ebnalwaled, Asmaa Sayed & Wesam Salem To cite this article: Hani Saber, Eman A. Alwaleed, K.A. Ebnalwaled, Asmaa Sayed & Wesam Salem (2017) Efficacy of silver nanoparticles mediated by Jania�rubens and Sargassum�dentifolium macroalgae; Characterization and biomedical applications, Egyptian Journal of Basic and Applied Sciences, 4:4, 249-255, DOI: 10.1016/j.ejbas.2017.10.006 To link to this article: https://doi.org/10.1016/j.ejbas.2017.10.006 © 2017 Mansoura University Published online: 08 Mar 2019. Submit your article to this journal Article views: 145 View related articles View Crossmark data Citing articles: 1 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=teba20 Egyptian Journal of Basic and Applied Sciences 4 (2017) 249–255 Contents lists available at ScienceDirect Egyptian Journal of Basic and Applied Sciences journal homepage: www.elsevier.com/locate/ejbas Full Length Article Efficacy of silver nanoparticles mediated by Jania rubens and Sargassum dentifolium macroalgae; Characterization and biomedical applications Hani Saber a, Eman A. Alwaleed a, K.A. Ebnalwaled b,c, Asmaa Sayed a, Wesam Salem a,⇑ a Botany Department, Faculty of Science, South Valley University, Qena 83523, Egypt Electronics & Nano Devices Laboratory, Physics Department, Faculty of Science, South Valley University, Qena 83523, Egypt c Egypt Nanotechnology Center (EGNC), Cairo University Sheikh Zayed Campus, 12588 Giza, Egypt b a r t i c l e i n f o Article history: Received 5 August 2017 Received in revised form 7 October 2017 Accepted 17 October 2017 Available online 31 October 2017 Keywords: Antibiofilm Jania rubens Pathogenic bacteria Sargassum dentifolium Silver nanoparticles a b s t r a c t Jania rubens and Sargassum dentifolium aqueous extracts were used as a reducing agent for the synthesis of silver nanoparticles (Ag-NPs). The prepared Ag-NPs have two plasmon absorption bands at 440 nm and 420 nm, with a direct band gap 2.25 eV and 2.38 eV for Ag-NPs/J. rubens and Ag-NPs/S. dentifolium respectively. From the FTIR results, the reduction has mostly been carried out by C@N, hydroxyl or sulfated polysaccharides groups present in J. rubens and S. dentifolium, respectively. TEM images shown that most particles are spherical in shape with no aggregations or debris were detected. The concentration of S. dentifolium/NPs showed approximately 2-fold than J. Rubens/NPs (470 and 240
103 NPs/ml) and an average particle size of 113 and 155 nm, respectively. A high repulsive and attractive forces between each nanoparticle were confirmed with an average zeta potential 24.7 and 28.2 mV for J. rubens/NPs and S. dentifolium/NPs, respectively. On the other hand, Ag-NPs concentrations of 104–105/ml were sufficient for killing Salmonella typhimurium, Enterobacter aerogenes, Pseudomonas aeruginosa, Escherichia coli, and the Gram-positive methicillin-resistant Staphylococcus aureus. Generally, both NPs showed reproducible, effective antibacterial activity with no significant differences between values of MIC and/or MBC for the two NPs against the tested pathogens. The results on biofilm formation implicate significant inhibition at the beginning of the adherence stage at various concentrations of Ag-NPs tested. Consequently, silver nanoparticles could be an effective antimicrobial agent without cause microbial resistance even after long-term usage. Ó 2017 Mansoura University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 1. Introduction Nano-biotechnology is an evolving field that has made its contribution to all domains of human life [1–5]. Several physicochemical approaches have been illustrated for the synthesis of metal nanoparticles [6–12]. A variety of green methods for synthesis of nanoparticles were needed because most of these means are inexpensive, non-toxic, eco-friendly and have easy production technology. The synthesis of new approaches for green nanoparticles from natural sources like plants [13,14] and algae [15–17]. For their easy availability, natural occurring, non-toxicity, the macroalgae have several potentials in the biosynthesis of silver nanoparticles such as eco-friendly, easy production technology and low costs [18]. El-Rafie et al. [1] demonstrated a fast, unexpensive, green easily amenable approach for biosynthesis of silver nanoparticles by reducing silver nitrate solution using marine macroalgae ⇑ Corresponding author. E-mail address: wesam.salem@svu.edu.eg (W. Salem). extract. Several macroalgae have been used for biosynthesis of Ag-NPs, particularly different Sargassum spp. [19,20] and Jania rubens [1]. It appears to be exceptionally sensible to trust silver nanoparticles biosynthesis as environmentally and ecologically safe as well as to enhance their antibacterial properties [14]. For metal nanoparticles, silver nanoparticles (Ag-NPs) have great efficacy as ideal antimicrobial agents [21]. Because of their antiseptic properties, the silver nanoparticles are widely used in the healthcare sector and industrial applications [22–25]. On the other hand, Microbial activity in the environment is the main source of drinking water and foods spoilage, it is often responsible for severe diseases including the food-born one. Among these pathogenic bacteria, the Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium and Staphylococcus aureus are biofilm forming organisms with a high multi-drug resistance against traditional antibiotics [26–30]. Antimicrobial agent against bacterial biofilm has been subjected to a considerable amount of work using green silver nanoparticles [13]. Although, a lot of work has been done on detecting the determinants of biofilm formation https://doi.org/10.1016/j.ejbas.2017.10.006 2314-808X/Ó 2017 Mansoura University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 250 H. Saber et al. / Egyptian Journal of Basic and Applied Sciences 4 (2017) 249–255 in vitro and, to some degree, the consequences of biofilms for persistence and pathogenicity in vivo [31].The biosynthesized nanosized silver particles have a strong antibacterial activity for both Gram negative and positive bacteria. This makes silver metal an ideal alternative for different aims in the medical and biotechnological fields and may lead to important results for facing pathogenic microorganism and reduce the prevalence of illnesses caused by these bacterial strains [13]. The killing mechanism of Ag-NPs is due to the formation of free radicals that facilitate the induction of membrane damaging molecules [32]. Antimicrobial activity of Ag-NPs largely has been studied with human pathogenic bacteria, mainly Escherichia coli and Staphylococcus aureus [33], Enterotoxigenic Escherichia coli (ETEC) and Vibrio cholerae [13]. Hence, the present study was carried out to understand the antibiofilm activity against some human pathogenic bacteria by (fullcharacterized) biosynthesized silver nanoparticles (Ag-NPs) using the aqueous extracts of two macro-algae, Jania rubens and Sargassum dentifolium which are the most common types of red and brown marine algae, respectively, present at the Red sea coast of Egypt. 2. Materials and methods 2.1. Macroalgae selection, collection and extract preparation Jania rubens and Sargassum dentifolium macro-algae were collected by hand picking from the red sea in Hurghada, Egypt during May 2015. Healthy algal samples were cleaned from epiphytes, extraneous matter and necrotic were removed. Samples were washed thoroughly with sea water then sterile distilled water, air dried, cut into small pieces and then ground in a tissue grinder (IKA A 10, Germany) until reaching fine powder shape. Dried seaweed powder (1 g) was mixed with 100 ml of distilled water and heated to 100 °C, then filtered through RotilaboÒ Tyb 601P filter paper. 2.2. Green synthesis of silver nanoparticles (Ag-NPs) Ag-NPs were essentially synthesized as previously described [19]. A volume of 50 ml of 1 mM AgNO3 solution was reacted with 50 ml of the aqueous extract of both algal extracts under continuous stirring at 45 °C. The solution changed color (brownish yellow to light purple) within 1 h, indicating the formation of Ag-NPs. For complete reaction, the obtained solution was left under stirring for a further 4 h. The Ag-NPs/J. rubens and/or S. dentifolium formed were separated from the residual seaweed by collecting the pellets after centrifugation at 6000 rpm/min for 10 min. The pellets were again suspended in double-distilled water and adjusted by adding 0.1 ml of phosphate buffer to the whole volume of physiological pH. 2.3. Characterization of nanoscale silver nanoparticles The phyto-reduction of silver ions was monitored by recording the UV–Vis spectrum with a computerized double beam UV-2300 spectrophotometer with 5 nm steps. Measurements were performed at room temperature, in quartz cells, using silver nitrate (1 mM) as a blank, at normal incidence in the wavelength range 200–1100 nm. The reproducibility of the data was checked by measuring several specimens. The size and shape of silver nanoparticles were observed at 70 kV using ‘‘JEOL-2010, Japan” transmission electron microscope (TEM) equipped with digital ‘‘Kodak MegaplusÒ 1.6i camera” with image analysis and processing software (AMT, USA). Samples were prepared by placing a drop of each solution on carbon-coated copper grid and drying at room temperature as previously described by Salem et al. [14]. The infra- red spectra of the macroalgae powder and of the green synthesized Ag-NPs were recorded on a Magna-FTIR 560 (USA) instrument at a resolution of 2 cm1 range from 4000 to 400 cm1 in KBr pellet using diffuse reflectance mode operated by Nicolet Omnic software as instructed by the manufacturers. The hydrodynamic diameters and Zeta potential of the biosynthesized Ag-NPs were measured using a Zetasizer Nano series compact scattering spectrometer (Malvern Instruments Ltd.; Malvern 6.32, UK). 2.4. Bacterial strains, culture conditions, and supplements Human-pathogenic bacteria including the Gram-negative Salmonella typhimurium (ATCC14028), Enterobacter aerogenes (ATCC13048), Pseudomonas aeruginosa (ATCC278223), Escherichia coli (ATCC 25922) and the Gram-positive methicillin-resistant Staphylococcus aureus (MRSA, ATCC43300). Bacterial strains were maintained on Tryptic Soy Agar (TSA) slants and incubated at 37 °C for 24–48 h [34]. Three replicates of each microorganism were set up. The inocula were spread over TSA plates (107 CFU). 2.5. Determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) Overnight (ON) cultures of the respective strains were grown in Tryptic Soy Broth (TSB) to an optical density at 595 nm (OD595) of 1. Subcultures 1:1000 in (TSB). Samples of 100 ll bacterial cultures were placed into 96-well plates (F bottom, Sterilin) and 10 ll of appropriate serial dilutions of Ag-NPs (NPs/ml) was added. After 24 h incubation in a humid chamber at 37 °C, the optical density (OD595) was measured using the ‘‘SPECTRONICÒ GENESYSTM 2PC” Spectrophotometer, Spectronic Instruments, USA. To confirm bacterial growth inhibition and determine lack of metabolic activity, 40 mL of p-iodonitrotetrazolium violet (INT, 0.2 mg/ml, Sigma– Aldrich) was added to microplate wells and re-incubated for 30 min at 37 °C [35]. The MIC in the INT assay was defined as the lowest concentration of NPs that prevented color change as described earlier [36]. Growth was defined by an at least 2-fold increase of the OD595 compared to the negative control (TSA only). Next, MBC testing was performed, the bactericidal effect was defined as a 99.9% decrease in CFU (3 logs) in the starting inoculum during a 24 h incubation. The MBC was determined by transferring 50 ml from each well of an overnight MIC plates to sterile (TSA) fresh plates. Viable colonies were counted after 24 h at 37 °C. The limit of detection for this assay was 101 CFU/mL. 2.6. Static biofilm assay Static biofilms were performed in microtiter plates by crystal violet staining essentially as previously published by Seper et al. [37], with some modifications. Briefly, the respective strains were grown overnight on (TSA) plates, suspended in (TSB), adjusted to an OD595 of 0.02. 130 ll of this dilution were placed in a 96 well microtiter plate (U bottom, Sterilin) for 24 h at 37 °C. After 24 h, 10 ll of Ag-NPs colloidal solutions with concentrations of 105 NPs/ml was added. The addition of 10 ll of the both macroalgae aqueous extracts served as control. Biofilm was stained with 0.1% crystal violet, solubilized in 96% ethanol and the OD595 was measured using InfiniteÒ F50 Robotic (Ostrich) Microplate Reader to quantify the amount of biofilm. 2.7. Statistical analysis Data were analyzed using the Mann-Whitney U test or a Kruskal-Wallis test followed by post hoc Dunn’s multiple comparisons. Differences were considered significant at P values of .05. For all statistical analyses, GraphPad Prism version 5 was used. H. Saber et al. / Egyptian Journal of Basic and Applied Sciences 4 (2017) 249–255 3. Results and discussion 3.1. Characterization of the nanoparticles 3.1.1. UV–Vis spectrophotometer analysis Silver nanoparticles (Ag-NPs) were synthesized according to established protocols [19] using dried seaweed powder from Jania rubens and Sargassum dentifolium macroalgae. Resulting in the two different sources of nanoparticles Ag-NPs/J. rubens and/or S. dentifolium. Throughout the study, NPs have been prepared several times without dramatic changes in yield or quality, suggesting a reproducible production of the NPs. The reaction was completed within 60 min which indicated by color changes of the silver nitrate solution after addition of aqueous extracts of both algae. In contrast, no change of color for the silver nitrate solution (control) without extracts. The intensity of colors steadily increased along the incubation period. Finally, Ag-NPs/J. rubens and/or S. dentifolium solutions exhibited a brownish yellow to light purple color respectively. The reduction of silver nitrate and excitation of surface plasmon resonance could be responsible for color reaction change [38]. UV–Vis spectrometer is spectral techniques are widely used to confirm the formation and structural characterization of nanoparticles in colloidal solution [39,40]. The UV–Vis spectroscopy was used for the confirmation of silver ions reduction by aqueous extracts to form Ag-NPs (Fig. 1A). A wavelength scan in the UV–Vis spectra revealed an absorption peak at approximately k = 440 for Ag-NPs/J. rubens (Fig. 1A). Furthermore, Ag-NPs/S. dentifolium exhibited characteristics absorption peaks at approximately k = 420 nm as previously published [19,41]. Optical absorption spectra of Ag-NPs are clearly dominated by surface plasmon within a shift to the red and/or blue end depends on particle size, morphology, the state of aggregation and the coating dielectric medium [42]. Interestingly, the prepared Ag-NPs have two plasmon absorption band, one at 295 nm for the both extracts, and the second at 440 nm and 420 nm for Ag-NPs prepared from J. Rubens and S. dentifolium extracts respectively. The plasmon absorption bands are the characteristics bands for Ag nanoparticles [42,43], which confirm that we obtain Ag nanoparticles. A surface plasmon absorption band appeared at 420 nm and 440 nm for AgNPs extracts indicating the presence of spherical or roughly spherical Silver nanoparticles [44]. As described in Fig. 1B, the transmittance for Ag-NPs decreases with the increase of the wavelength till 440 nm after this wavelength the transmittance increases, these results indicates transparent Ag-NPs. The value and nature of the optical band gap could be detected from the absorption, which corresponds to electron excitation from the valence to conduction bands. The relation between the absorption coefficients (a) and the incident photon energy (hm) can be written as (ahm)1/n = A (h 251 m  Eg) [45] where A is a constant and Eg is the band gap of the material and exponent n depends on the type of transition. For direct allowed n = 1/2, indirect allowed transition, n = 2, and for direct forbidden, n = 3/2. Fig. 1C depicts the relations between (ahm)2 and photon energy to determine the direct allowed band gap. These results clearly indicate that the direct band gap is 2.25 eV and 2.38 eV for Ag-NPs/J. rubens and Ag-NPs/S. dentifolium respectively. 3.1.2. FTIR studies The FTIR spectra were used to identify the possible functional biomolecules responsible for the reduction of the Ag+ ions and capping of the macroalgae formed Ag-NPs. Fig. 2 shows the FTIR spectra of J. rubens (Fig. 2A) and/or S. dentifolium (Fig. 2B) aqueous extracts and its bio-synthesized Ag-NPs. In the FTIR spectrum of J. Rubens extract, the signal at 1635 cm1 corresponded to asymmetric stretching vibration of a C@N and band at 2834 and/or 2920 cm1 attributed to symmetric CH- aliphatic vibration associated with aliphatic groups [46] or the CAOH, which disappeared after synthesis of Ag-NPs. This specified the involvement of C@N or hydroxyl groups in the reduction process of Ag-NPs. In addition, the peaks at 3420 cm1 (OH stretching) was also detected. After reduction of AgNO3, the decreases in intensity at 3397 cm1 imply the involvement of the OH group in the reduction process. This is further confirmed with a reduction in PH of solution during the reaction. The sulfated polysaccharides peaks pointed out the involvement of sulfate groups in the biosynthesis of silver nanoparticles [19]. In agreement with that, Mahdavi et al. [47] and Venkatpurwar and Pokharkar’s [48] stated that the sulfated polysaccharides in marine algae S. muticum and Porphyra vietnamensis had strong ability to synthesis of NPs. For FTIR spectra of S. dentifolium (Fig. 2B), the peaks at 1426 cm1 indicate the C@C Fig. 2. Fourier Transform Infrared Spectroscopy (FTIR) Spectrum of biosynthesized silver nanoparticles. (A) Ag-NPs/Jania rubens (black line) and its algal extract (red line) (B) Ag-NPs/Sargassum dentifolium (black line) and its algal extract (red line) showing bioactive functional groups. Fig. 1. Spectra analysis for biosynthesized silver nanoparticles (Ag-NPs). Shown are the UV–Vis absorption spectra from 200 to 800 nm of (A) Ag-NPs/Jania rubens (green line) and Ag-NPs/Sargassum dentifolium (brown line); (B) the dependence of transmittance on the wave length. (C) The relations between (ahm)2 and photon energy to determine the directly allowed band gap for both synthesized Ag-NPs. 252 H. Saber et al. / Egyptian Journal of Basic and Applied Sciences 4 (2017) 249–255 groups derived from aromatic rings that are present in the S. dentifolium aqueous extract. Another peak at 1634 cm1 is attributed to the stretching vibration of (NH) C@O group that is characteristic of proteins was shifted and became shorter after synthesis of AgNPs, approved that a member of (NH) C@O group is contributed in capping the nanoparticles synthesis. Hence, the FTIR results elucidated that the Ag-NPs were successfully synthesized and coated with bio-compounds present in both algal extracts by using a green approachs. 3.1.3. TEM and particles size distribution analysis TEM measurements of the synthesized nanoparticles give a clear idea of the shape and size of the Ag-NPs produced extracellularly by J. Rubens and S. dentifolium extracts. As seen in Fig. 3 most the particles are spherical in shape. Few ellipsoidal and irregular silver nanoparticles can also be noticed. The exact size distribution and concentration of NPs in the preparations used in the assays throughout the study was determined by Zetasizer Nano series compact scattering spectrometer (Fig. 3). In general, S. dentifolium/NPs preparations showed approximately 2-fold concentrations than J. Rubens/NPs. (470 and 240
103 NPs/ml respectively), and average particle size of 113–155 nm. No aggregations or debris were detected by visualization of the nanoparticles within the TEM images (Fig. 3), which indicates the purity and uniformity of the colloidal solution of nanoparticles. Control of the size and structure of the resultant nanoparticles could be related to the interactions between bio-compounds such as polysaccharides, proteins, polyphenols and phenolic compounds and metal atoms [49]. 3.1.4. Zeta potential (ZP) Fig. 3 shows the ZP of the bio-synthesized Ag-NPs. The average ZP was 24.7 and 28.2 mV for Ag-NPs/J. rubens (Fig. 3A) and AgNPs/S. dentifolium (Fig. 3B) respectively. From the average ZP values, it is suggested that the bio-synthesized Ag-NPs were stable and wrapped with anionic compounds and responsible for electro- static stabilization. This might be achieved by the help of high repulsive and attractive forces between nanoparticles [50]. Negative charge on surface of nanoparticles results in repulsion among the nanoparticles leading to stability of nanoparticles in cell culture media [51]. 3.2. Efficacy of Ag-NPs against some human pathogenic bacteria The antibacterial activities of the two synthesized Ag-NPs mediated by J. rubens and S. dentifolium extracts were studied against the most dominant bacterial causative agents for a human. Minimal inhibitory concentration (MIC) and/or minimal bactericidal concentration (MBC) assays were statistically analyzed for that target (Fig. 4). For MIC, a colorimetric INT-formazan assay, which allows detection of viable bacteria by their respiratory activity was assessed [52,53]. Interestingly, no significant differences between values of MIC and/or MBC for the two NPs against the tested pathogens (Fig. 4). In detail, Ag-NPs concentrations of 104– 105/ml were sufficient for the killing of all the tested Gramnegative and positive bacteria (Fig. 4A and B). Silver nanoparticles can cause growth inhibition or cell lysis via various mechanisms [32,54]. The lethal effect of silver for bacteria can also be elucidated by thiol group reactions that inactivate enzymes [55,56]. Also, Steuber et al. [57] proposed a mechanism for Ag+ action in Vibrio alginolyticus involving the direct displacement of FAD from the holoenzyme Na+-NQR, which results in loss of enzyme activity. In addition, silver treatments inhibits DNA replication, ribosomal molecules and expression of extracellular proteins, moreover, it could be interferes with the respiratory chain of microorganisms [56,58,59]. 3.2.1. Macroalgae extracts and Ag-NPs have inverse effects on biofilm formation Biofilm formation by pathogenic bacteria has been the area of a severity amount of experimental work. Since biofilms are generally known to promote resistance to several antimicrobial agents, we Fig. 3. TEM images with the corresponding size distribution and zeta potential for biosynthesized silver nanoparticles. (A) Ag-NPs/Jania rubens and (B) Ag-NPs/Sargassum dentifolium. H. Saber et al. / Egyptian Journal of Basic and Applied Sciences 4 (2017) 249–255 Fig. 4. Efficacy of Ag-NPs expressed as MIC and MBC assay reduction. MIC90 determination of Ag-NPs against some pathogenic bacteria (A) Ag-NPs/Jania rubens (B) Ag-NPs/Sargassum dentifolium were determined by measuring OD600 nm for MIC by tetrazolium reduction assay (gray) and were confirmed as MBC by colony count (white). Shown are the medians from at least eight independent measurements. The error bars indicate the interquartile range. analyzed the impact of Ag-NPs on human-pathogenic bacteria such as S. typhimurium, E. aerogenes, P. aeruginosa, E. coli and the Grampositive methicillin-resistant S. aureus. Therefore, we allowed the five tested pathogens to from static biofilms for 24 h before AgNPs were added. The addition of J. Rubens and S. dentifolium extracts served as a positive control, respectively. Finally, after an additional incubation period of 24 h the biofilm amount was quantified by crystal violet staining (Fig. 5). Among the five tested bacterial strains, the addition of Ag-NPs significantly reduced the biofilm amount compared to control, the exception is S. aureus in the case of Ag-NPs/S. dentifolium. Interestingly, the two Ag-NPs showed a highly significant difference compared to the original 253 macroalgal extracts, except for P. aeruginosa (Fig. 5). Notably, the two macroalgal extracts showed no change in the biofilm formation for all five tested pathogens compared to control (original biofilm), the exception is E. coli with both extracts, and S. typhimurium with J. Rubens extract. Although both NPs exhibited robust antibacterial activity in the MIC by INT assays and MBC; the Ag-NPs/J. rubens generally showed a slightly higher efficacy against all pathogens compared to Ag-NPs/S. dentifolium that affected only Gram-negative bacteria. At 24 h of incubation, the Ag-NPs depicted the weak adherence and disintegrated biofilm of the test bacterial strains, in control, the strong adhering ability of bacterial biofilm led to the development of dense biofilm formation on the 96microplate was observed. Thus Ag-NPs were active positively in clearing the establishment of the bacterial biofilm [60]. Also, the protein biosynthesis of stabilized Ag-NPs coated with polycaprolactam (polymer) proved to be a good anti-biofilm agent against pathogenic bacteria. [61]. The same technique was adopted earlier to measure the biofilm inhibition and adherences against Streptococcus pyogenes [62], Staphylococcus aureus [63,64], and E. coli [65] using different bacterial extracts. The coated nanoparticles on the surfaces, surfactants, and enzymes inhibited the bacterial biofilm formation [66] Same results were recorded earlier as against Vibrio cholerae and enterotoxigenic Escherichia coli using Ag-NPs synthesized from Calotropis procera [13]. Noteworthy, bacterial biofilms cause chronic infections because they show increased resistance against antibiotics, chemicals and phagocytosis [67]. Thus, the failer of antibiotic penetration within the bacterial biofilm matrix resulted to the bacterial biofilms resistence against these antibiotics [68]. The above-mentioned attributes of bacterial biofilms place them along with the most dangerous problems, which medicine is currently challenged. The results implicate that the biofilm formation was possibly inhibited at the beginning of the adherence stage at various concentrations of Ag-NPs tested. Consistent with our results, Ag-NPs have been recently shown to inhibit and reduce biofilm formations of several bacterial species [69]. 4. Conclusion Ag-NPs offer an inexpensive alternative approach to reducing the infectious dose of the most common food-born pathogens. The quick and ready synthesis of nanoparticles does not require specially trained staff or expensive tools and is likely to be Fig. 5. Alterations of static biofilm formation treated with Ag-NPs. The biofilm formation capacity of some pathogenic bacteria was quantified after 24 h post inoculations with Ag-NPs/Jania rubens (JNPs) and Ag-NPs/Sargassum dentifolium (SNPs) compared to macro-algae extracts; J. rubens (J) or S. dentifolium (S) under static conditions by crystal violet staining and subsequent determination of the OD595. Shown are the medians from at least eight independent measurements. The error bars indicate the interquartile range. 254 H. 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