Pseudosterigmatospora motuoensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov.

MycoBank number: MB 832545; Index Fungorum number: IF 832545; Facesoffungi number: FoF; Figs 11D and 14P

Etymology: the specific epithet motuoensis refers to the geographic origin of the type strain, Motuo, Tibet.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ovoid and ellipsoidal, 2.2 – 3.0 × 3.7 – 5.3 μm and single, budding is polar (Fig. 14P), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. One or more conidia are produced on each stalk-like conidiophore. Conidiophore is single or multiple, usually bifurcate, somewhat trifurcate. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed and weak), L-sorbose (delayed and weak), sucrose, maltose (delayed and weak), trehalose, melezitose, ethanol, D- mannitol, D-glucitol and salicin (delayed and weak) are assimilated as sole carbon sources. Cellobiose, lactose, meli- biose, raffinose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, N-Acetyl- D-glucosamine, methanol, glycerol, erythritol, ribitol, galactitol, Methyl-α-D-glucoside, D-gluconate, DL-lactate, succinate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate, sodium nitrite (delayed and weak), L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride (delayed and weak) are assimilated as sole nitrogen sources. Maximum growth temperature is 26 – 27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Typus: China, Motuo, Tibet, obtained from a leaf of Achyrospermum wallichianum, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5816T preserved in a metabolically inactive state, ex- type CBS 15591 = XZ119B3).

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.

Fig. 14.