Investigation of the Copper Requirements of the Metallophyte Liverworts Cephaloziella nicholsonii Douin and C. massalongoi (Spruce) Müll.Frib
Abstract
:1. Introduction
2. Results
2.1. Soil Analysis
2.2. Growth through Course of Experiment
2.3. Cephaloziella nicholsonii
2.4. Cephaloziella massalongoi
2.5. Tolerance Indices of C. nicholsonii and C. massalongoi
3. Discussion
4. Materials and Methods
4.1. Sampling and Initiation into Culture
4.2. Growth Media for Experimental Investigation
4.3. Experimental Procedure
4.4. Soil Sampling and Analysis
4.5. Data Handling and Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | Population | Grid Ref. | Bedrock | Metal(s) Mined | Yrs Worked | pH | Cu | Pb | Zn |
---|---|---|---|---|---|---|---|---|---|
Cephaloziella nicholsonii | Caim, Co. Waterford | S885408 | Metamorphic | Cu & Pb | 1815–1855 | 5.83 | 2513 | 37,004 | 1495 |
Mountain Mine, Allihies, Co. Cork | V589456 | Sandstone | Cu | 1813–1918 | 5.81 | 9877 | 129 | 162 | |
South Caradon Mine, Cornwall | SX264698 | Granite | Cu | 1837–1885 | ND | ND | ND | ND | |
Cephaloziella massalongoi | Ross Island, Co. Kerry | V945880 | Limestone | Cu & Pb | 1804–1810 | 6.28 | 3815 | 9911 | 12,647 |
Glendasan, Co. Wicklow | T098981 | Granite, Schist | Pb | 1807–1900 | 7.03 | 478 | 30,522 | 14,502 | |
Gunnislake Clitters, Cornwall | SX422720 | Granite | Cu, Sn, Ar & W | 1820–1919 | ND | ND | ND | ND | |
Bunmahon, Co. Waterford | X443986 | Slate | Cu | 1730–1907 | 5.80 | 12,528 | 34 | 81 | |
Mountain Mine, Allihies, Co. Cork | V590458 | Sandstone | Cu | 1813–1918 | 5.99 | 6019 | 52 | 32 |
Source | Degrees of Freedom | Sum of Squares | Mean Square | F-Ratio | p |
---|---|---|---|---|---|
Constant | 1 | 162.563 | 162.563 | 239.34 | ≤0.0001 |
Population | 2 | 23.2821 | 11.641 | 17.139 | ≤0.0001 |
Treatment | 6 | 78.5549 | 13.0925 | 19.276 | ≤0.0001 |
Population × Treatment | 12 | 26.9134 | 2.24278 | 3.3021 | 0.0020 |
Error | 42 | 28.5267 | 0.679206 | ||
Total | 62 | 157.277 |
Source | Degrees of Freedom | Sum of Squares | Mean Square | F-Ratio | p |
---|---|---|---|---|---|
Constant | 1 | 244.864 | 244.864 | 122.65 | ≤0.0001 |
Population | 2 | 215.456 | 35.9094 | 17.986 | ≤0.0001 |
Treatment | 6 | 188.919 | 94.4595 | 47.312 | ≤0.0001 |
Population × Treatment | 12 | 41.3471 | 3.44559 | 1.7258 | 0.0954 |
Error | 42 | 83.8533 | 1.99651 | ||
Total | 62 | 529.576 |
Treatment | Cephaloziella nicholsonii | Cephaloziella massalongoi | p |
---|---|---|---|
3 ppm | 0.79 (0.19) | 0.44 (0.06) | 0.26 (ns) |
6 ppm | 0.81 (0.23) | 0.15 (0.08) | 0.007 ** |
12 ppm | 0.51 (0.11) | 0.08 (0.04) | 0.005 ** |
24 ppm | 0.22 (0.07) | 0.10 (0.05) | 0.09 (ns) |
48 ppm | 0.03 (0.01) | 0.09 (0.04) | 0.67 (ns) |
96 ppm | 0.14 (0.04) | 0.05 (0.02) | 0.09 (ns) |
Chemical Name | Chemical Formula | mg Litre−1 | |
---|---|---|---|
Macroelements | Ammonium nitrate 1 | NH4NO3 | 125.0 |
Potassium dihydrogen phosphate 1 | KH2PO4 | 500.0 | |
Magnesium sulphate 1 | MgSO4 | 120.0 | |
Calcium chloride 1 | CaCl2 | 20.0 | |
Microelements | Sequestrene 330 1 | C14H18FeN3Na2O10·× H2O | 14.0 |
Boric Acid 1 | H3BO3 | 11.4 | |
Zinc sulphate 2 | ZnSO4·7H2O | 8.82 | |
Copper sulphate 2 | CuSO4·5H2O | 1.57 | |
Manganese sulphate 2 | MnSO4·4H2O | 0.0223 | |
Sodium molybdate 2 | Na2MoO4·2H2O | 0.0250 | |
Cobalt chloride 2 | CoCl2·6H2O | 0.0025 |
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Campbell, C.; Kelly, D.L.; Smyth, N.; Lockhart, N.; Holyoak, D.T.; Long, D. Investigation of the Copper Requirements of the Metallophyte Liverworts Cephaloziella nicholsonii Douin and C. massalongoi (Spruce) Müll.Frib. Plants 2023, 12, 2265. https://doi.org/10.3390/plants12122265
Campbell C, Kelly DL, Smyth N, Lockhart N, Holyoak DT, Long D. Investigation of the Copper Requirements of the Metallophyte Liverworts Cephaloziella nicholsonii Douin and C. massalongoi (Spruce) Müll.Frib. Plants. 2023; 12(12):2265. https://doi.org/10.3390/plants12122265
Chicago/Turabian StyleCampbell, Christina, Daniel L. Kelly, Noeleen Smyth, Neil Lockhart, David T. Holyoak, and David Long. 2023. "Investigation of the Copper Requirements of the Metallophyte Liverworts Cephaloziella nicholsonii Douin and C. massalongoi (Spruce) Müll.Frib" Plants 12, no. 12: 2265. https://doi.org/10.3390/plants12122265