ANTI CARDIOLIPIN ANTIBODY
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Anti cardiolipin antibody
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ANTI CARDIOLIPIN ANTIBODY
- 2. ACA is form of antibody directed against CARDIOLIPIN. Cardiolipin is an important component of the inner mitochondrial membrane, where it constitutes about 20% of the total lipid composition. when immune system recognize cardiolipin as a foreign substance, ACA is produced and results in auto immune disorder.
- 3. The ACA test detects the autoantibodies present in an individual’s blood serum. The common test used for detecting and quantifying ACAs is ENZYME LINKED IMMUNOSORBANT ASSAY(ELISA).
- 4. 1st step : Sample addition – 490µl diluent + 10µl sample Incubation – 30 minutes at room temperature. Washing – 3 times with wash buffer either manually or automatically.
- 5. 2nd step : Addition of conjugate – 100µl conjugate Incubation – 30 minutes at room temperature. Washing – washed with wash buffer.
- 6. 3rd step : Adding TMB solution – 100 µl substrate Adding stop solution - Stopping the enzymatic reaction.
- 8. Qualitative results provide a simple positive or negative result. In quantitative ELISA, measured at 420nm in colorimeter.
- 9. oAnti cardiolipin antibodies are found in several diseases, including syphilis, antiphospholipid syndrome, livedoid vasculitis, vertebrobasilar insufficiency, and Systemic Lupus Erythematosus(SLE). oThey are form of anti-mitochondrial antibody. oIn SLE, anti-DNA antibodies and anti-cardiolipin antibodies may be present individually or together