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Biodiversity and the Healthy Society
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Stas BS-MLS (STAS111)
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Our Lady of Fatima University
Academic year: 2022/2023
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Research Outline on the impacts of Bt Corn
Research Topic: IMPACT OF BT CORN ON THE DIPLOPOD ALLAJULUS LATESTRIATUS
Name: DARREN B. BEQUILLA Section: BSITM-TTO 3YA-
Title and
Objectives/ Problems
Instruments and
Participants (Sampling)
Data Collection and
Analysis
Key Findings Conclusions and
Recommendations
Insights
DOI and
References
(APA)
Title: Impact of Bt corn on
the diplopod Allajulus
latestriatus
Objective:
To develop or contain a
genetically microorganism
that can produce proteins that
are toxic to pesters to improve
or have better crop
production with less
environmental defilement but
with elevated economic
benefits to farmers.
Statement of the problem:
1. Possible effects of Bt corn
on the detritophagous
Allajulus latestriatus
2. The effectivity of it to
control pests effectively.
In this study, laboratory feeding
experiments were conducted to
detect possible effects of Bt corn
on the detritophagous Allajulus
latestriatus (Diplopoda,
Julidae). We tested the
transgenic corn N4640Bt
(expressing the Bt protein
Cry1Ab), the corresponding
non-transgenic isoline, and two
further conventional corn
varieties. Furthermore, a
toxicity test was performed, in
which purified Cry1Ab was
added to a corn leaf diet in
different concentrations. Cry1Ab
concentrations in corn leaves,
corn leaf diet, faeces and
diplopods were quantified,
applying an enzyme-linked
immunosorbent assay (ELISA).
The researchers
performed bioassays
using the European
Corn Borer Ostrinia
nubilalis to test the
insecticidal activity of
different samples.
Bioassays proved that
the Bt protein in
transgenic leaves and in
faeces of diplopods was
still insecticidal. The
toxicity test indicated
that Cry1Ab excretion
in the faeces is linearly
correlated to the
Cry1Ab uptake through
the diet.
The findings suggest
that Bt corn and
Cry1Ab will not harm
A. latestriatus.
However, this
diplopod excretes
considerable
amounts of Bt
protein with its
faeces. The Bt protein
is still insecticidally
active and, thus,
becomes available to
other soil organisms.
Potential effects of Bt-
corn on diplopods had
hitherto not been
investigated. Diplopods
are certainly not the
most important group of
macro-decomposers of
plant litter but they are
widely spread in the
cultural landscape and
regularly occur in
cornfields. Our results
show that A. latestriatus
is not affected by Bt
corn and the toxicity
test demonstrated that
even very high Cry1Ab
concentrations do not
lead to recognizable
effects. Furthermore,
diplopods as macro-
decomposers prefer to
feed on
Based on research, the use of BT Corn
can help increasing the income of our
farmers as the discovery of it can
improve the crop production. Since it is
improved through biotechnology, it can
also help to lessen soil pollution
compare to using pesticides on our crops
however looking on the other side of
using BT corn, it is for sure would cause
more money for consumer when it
comes to purchasing BT crops compare
to those naturally grown crops that we're
used to and there's also posibility that
time will come for pests or any other
pesterer to adapt or develop their own
resistance with the effect of this. The
possiblity of having less protein or
nutrients from BT crops compare to
those naturally grown crops shoukd also
be consider though research says that BT
crops still can provide protein to
humang being.
Weber, M. A., &
Nentwig, W.
(2006). Impact of
Bt corn on the
diplopod Allajulus
latestriatus.
Pedobiologia,
50(4), 357–
368:doi
g/10.1016/j
i.2006.
Research Outline on the impacts of Roundup Ready soybean
Research Topic: DISTRIBUTION AND DEGRADATION OF DNA FROM NON-GENETICALLY AND GENETICALLY MODIFIED
SOYBEAN (ROUNDUP READY): IMPACT OF SOYBEAN PROTEIN CONCENTRATE AND SOYBEAN PROTEIN ISOLATE PREPARATIO N
Name: DARREN B. BEQUILLA Section: BSITM-TTO 3YA-
Title and
Objectives/ Problems
Instruments and
Participants (Sampling)
Data Collection and
Analysis
Key Findings Conclusions and
Recommendations
Insights
DOI and
References
(APA)
Title: Distribution and
degradation of DNA from
non-genetically and
genetically modified soybean
(Roundup Ready): Impact of
soybean protein concentrate
and soybean protein isolate
preparation
Objective :
1. The purpose was to
investigate the impact of SPC
and SPI preparation on the
distribution and degradation
of the genomic DNA, and
their influence on GMO
quantification, systematically.
Changes in the content of
genomic DNA, length
distributions of the internal
reference target and
transgenic target, and
variations in transgenic
contents of samples
collected during the
preparation of SPC and SPI
were estimated in this
study.
making degradation of
recombinant DNA an
indispensable aspect for the
risk assessment concerning
GM foods (Bauer, Weller,
Hammes, & Hertel, 2003).
Therefore, it is of great
significance to survey the
changes in genomic DNA in
RRS as well as the major
factors affecting DNA
degradation and transgenic
component quantification
during food processing
The results showed
that the mass content
of the used internal
reference target
decreased after
grinding, mechanical
manipulation, and
the addition of
coagulant, indicating
the negative effects of
mechanical force and
chemical reagents on
DNA and variations
in RRS% during tofu
production.
In this study, we
systematically demonstrated
for the first time the
distribution and degradation
of the internal reference
LECTIN target and
transgenic CP4 EPSPS target
from all of the products and
by-products, and the
variations in transgenic
contents during non-GM and
GM SPC and SPI
preparations. Both
mechanical treatments such
as dehulling and chemical
reagent treatments such as
hexane extraction, ethanol
extraction, alkali dissolution,
and acid precipitation were
involved in the
In genetically modified soybeans,
the distribution and degradation of
DNA in these soybeans can be
influenced by the genetic
modification process, as well as by
the processing steps used to
prepare SPC and SPI since it is
proven and tested that the DNA
content of SPC and SPI derived
from genetically modified
soybeans can vary widely,
depending on factors such as the
source of the soybeans, the specific
genetic modification used, and the
processing conditions. Other
people says that this is still an area
of active research since DNA
content of these products may be
lower in genetically modified
soybeans therefore more research
is needed to fully understand the
implications of these findings for
human health and safety.
Du, Y., Chen, F.,
Bu, G., & Zhang,
L. (2021).
Distribution and
degradation of
DNA from non-
genetically and
genetically
modified soybean
(Roundup Ready):
Impact of soybean
protein
concentrate and
soybean protein
isolate
preparation. Food
Chemistry, 335,
127582.
doi/
.1016/j.
2020.
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Biodiversity and the Healthy Society
Course: Stas BS-MLS (STAS111)
272 Documents
Students shared 272 documents in this course
University: Our Lady of Fatima University
Was this document helpful?
Research Outline on the impacts of Bt Corn
Research Topic: I MPACT OF BT CORN ON THE DIPLOPOD ALLAJULUS LATESTRIATUS
Name: DARREN B. BEQUILLA Section: BSITM-TTO 3YA-1
Title and
Objectives/ Problems
Instruments and
Participants (Sampling)
Data Collection and
Analysis Key Findings Conclusions and
Recommendations Insights
DOI and
References
(APA)
Title: Impact of Bt corn on
the diplopod Allajulus
latestriatus
Objective:
To develop or contain a
genetically microorganism
that can produce proteins that
are toxic to pesters to improve
or have better crop
production with less
environmental defilement but
with elevated economic
benefits to farmers.
Statement of the problem:
1. Possible effects of Bt corn
on the detritophagous
Allajulus latestriatus
2. The effectivity of it to
control pests effectively.
In this study, laboratory feeding
experiments were conducted to
detect possible effects of Bt corn
on the detritophagous Allajulus
latestriatus (Diplopoda,
Julidae). We tested the
transgenic corn N4640Bt
(expressing the Bt protein
Cry1Ab), the corresponding
non-transgenic isoline, and two
further conventional corn
varieties. Furthermore, a
toxicity test was performed, in
which purified Cry1Ab was
added to a corn leaf diet in
different concentrations. Cry1Ab
concentrations in corn leaves,
corn leaf diet, faeces and
diplopods were quantified,
applying an enzyme-linked
immunosorbent assay (ELISA).
The researchers
performed bioassays
using the European
Corn Borer Ostrinia
nubilalis to test the
insecticidal activity of
different samples.
Bioassays proved that
the Bt protein in
transgenic leaves and in
faeces of diplopods was
still insecticidal. The
toxicity test indicated
that Cry1Ab excretion
in the faeces is linearly
correlated to the
Cry1Ab uptake through
the diet.
The findings suggest
that Bt corn and
Cry1Ab will not harm
A. latestriatus.
However, this
diplopod excretes
considerable
amounts of Bt
protein with its
faeces. The Bt protein
is still insecticidally
active and, thus,
becomes available to
other soil organisms.
Potential effects of Bt-
corn on diplopods had
hitherto not been
investigated. Diplopods
are certainly not the
most important group of
macro-decomposers of
plant litter but they are
widely spread in the
cultural landscape and
regularly occur in
cornfields. Our results
show that A. latestriatus
is not affected by Bt
corn and the toxicity
test demonstrated that
even very high Cry1Ab
concentrations do not
lead to recognizable
effects. Furthermore,
diplopods as macro-
decomposers prefer to
feed on
Based on research, the use of BT Corn
can help increasing the income of our
farmers as the discovery of it can
improve the crop production. Since it is
improved through biotechnology, it can
also help to lessen soil pollution
compare to using pesticides on our crops
however looking on the other side of
using BT corn, it is for sure would cause
more money for consumer when it
comes to purchasing BT crops compare
to those naturally grown crops that we're
used to and there's also posibility that
time will come for pests or any other
pesterer to adapt or develop their own
resistance with the effect of this. The
possiblity of having less protein or
nutrients from BT crops compare to
those naturally grown crops shoukd also
be consider though research says that BT
crops still can provide protein to
humang being.
Weber, M. A., &
Nentwig, W.
(2006). Impact of
Bt corn on the
diplopod Allajulus
latestriatus.
Pedobiologia,
50(4), 357–
368.https://doi.or
g/10.1016/j.pedob
i.2006.06.004
