Purpose

Mesocestoides spp. are Cyclophyllidean tapeworms with zoonotic importance. The current study aimed to investigate the molecular characteristics of Mesocestoides larvae (tetrathyridium) isolated from the abdominal cavity of persion jird, Meriones persicus, and from the liver of grey hamster, Cricetulus migratorius, in Ardabil Province, northwest Iran.

Methods

Genomic DNA of the isolates of Mesocestoides tetrathyridium were extracted, and mitochondrial gene of cytochrome-c oxidase subunit1 (cox1) was amplified. Sequencing of PCR products were performed and phylogenic analysis was run using MEGA 6.0 software.

Results

Both isolates were identified as Mesocestoides litteratus, showing high identity with M. litteratus sequences available in GenBank. Also, they had 100% homology to each other. Intra-species variation within isolates of M. litteratus were 0–2.4%. The phylogenetic reconstruction based on the partial sequence of the cox1 gene showed that our sequences of M. litteratus were clustered with M. litteratus isolates from Slovakia, Netherlands, Germany and Italy.

Conclusion

This is the first molecular description of M. litteratus from M. persicus and C. migratorius. Phylogenetic analysis illustrated that M. litteratus isolates of the current study had very high identities with the isolates of this species from other countries.

中文翻译：

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Mesocestoides litteratus 的分子特征（Cestoda：Cyclophyllidea：Mesocestoididae）Tetrathyridium 从伊朗的两种啮齿动物中分离出来

目的

Mesocestoides属。是具有人畜共患重要性的环叶绦虫。本研究旨在调查从伊朗西北部阿尔达比勒省的沙鼠Meriones persicus腹腔和灰色仓鼠Cricetulus migratorius的肝脏中分离出来的Mesocestoides幼虫（tetrathyridium）的分子特征。

方法

提取Mesocestoides tetrathyridium分离株的基因组DNA ，扩增细胞色素-c氧化酶亚基1（cox1）线粒体基因。对 PCR 产物进行测序，并使用 MEGA 6.0 软件进行系统发育分析。

结果

两种分离株均被鉴定为Mesocestoides litteratus ，与 GenBank 中可用的M. litteratus序列具有高度同一性。此外，它们彼此具有 100% 的同源性。M. litteratus分离株的种内变异为 0–2.4%。基于 cox1 基因部分序列的系统发育重建表明，我们的M. litteratus序列与来自斯洛伐克，荷兰，德国和意大利的M. litteratus分离物聚类。

结论

这是M. persicus和C. migratorius对M. litteratus的首次分子描述。系统发育分析表明，当前研究的M. litteratus分离株与来自其他国家的该物种的分离株具有非常高的同一性。