Marron, freshwater crayfish Cherax tenuimanus - Department of ...
Marron, freshwater crayfish Cherax tenuimanus - Department of ...
Marron, freshwater crayfish Cherax tenuimanus - Department of ...
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<strong>Marron</strong>, <strong>freshwater</strong> <strong>crayfish</strong> <strong>Cherax</strong> <strong>tenuimanus</strong><br />
1 Taxonomy<br />
Species: <strong>Cherax</strong> <strong>tenuimanus</strong> (Smith 1912)<br />
Family:<br />
Order:<br />
Class:<br />
Parastacidae<br />
Decapoda<br />
Crustacea<br />
Figure 1.<br />
Image <strong>of</strong> <strong>Cherax</strong> <strong>tenuimanus</strong> (Source: OpenCage, Wikimedia Commons).<br />
The marron <strong>crayfish</strong> <strong>Cherax</strong> <strong>tenuimanus</strong> is a robust <strong>freshwater</strong> species with a distinct prominence<br />
running back from the postorbital spine. In addition, the rostrum is characterised by lateral<br />
serrations present both sides, that ends in a sharp spine (Picker & Griffiths 2011). There has been<br />
some debate recently about the existence <strong>of</strong> two distinct species <strong>of</strong> marron <strong>crayfish</strong>. Genetic studies<br />
(involving allozyme data) have demonstrated that one <strong>of</strong> these forms (the hairy marron) is restricted<br />
to the Margaret River system while the other form (smooth marron) is the one that has been widely<br />
distributed as an aquaculture species (Austin & Ryan 2002). The authors proposed naming these two<br />
genetically distinct forms <strong>Cherax</strong> <strong>tenuimanus</strong> and <strong>Cherax</strong> cainii, respectively (Austin & Ryan 2002).<br />
However, these names are currently under review.<br />
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2 Natural distribution and habitat<br />
The hairy marron C. <strong>tenuimanus</strong> is native to south-western Australia (Figure 2). This species is<br />
considered Critically Endangered on the International Union for Conservation <strong>of</strong> Nature (IUCN) Red<br />
List in its native range (Austin & Bunn 2010) and found only in the upper Margaret River in the<br />
south-west <strong>of</strong> Western Australia (Cubitt 1985). It is thought that C. <strong>tenuimanus</strong> occupies an area <strong>of</strong><br />
approximately 10 km². However, the smooth marron C. cainii (the sub-species farmed globally) has a<br />
wider natural distribution and also appears to be moving into hairy marron territory (whereas<br />
previously the two sub-species were geographically distinct). Hybridisation between the two is<br />
causing the gradual displacement <strong>of</strong> the hairy marron (TSSC 2005). For the purposes <strong>of</strong> this study,<br />
we shall consider both C. <strong>tenuimanus</strong> and C. cainii as C. <strong>tenuimanus</strong>.<br />
Figure 2.<br />
Native (green) and introduced (red) ranges <strong>of</strong> C. <strong>tenuimanus</strong> globally (Source: M. Picker & C. Griffiths)<br />
C. <strong>tenuimanus</strong> prefer the sandy bottoms <strong>of</strong> rivers or dams, where they can find shelter from<br />
predators and access to accumulated organic matter, but can survive in a variety <strong>of</strong> habitats (TSSC<br />
2005).<br />
3 Biology<br />
3.1 Diet and mode <strong>of</strong> feeding<br />
<strong>Cherax</strong> <strong>tenuimanus</strong> is an omnivore and scavenger, feeding on dead plants and other forms <strong>of</strong> organic<br />
detritus (Read 1985). However, it will also consume living aquatic plants (Coetzee 1985).<br />
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3.2 Growth<br />
The marron can grow to a maximum <strong>of</strong> approximately 40 cm and weigh 2.5 kg (Picker & Griffiths<br />
2011). Under culture conditions it can attain 2 to 3 tons/ha/annum (Read 1985) but in natural<br />
conditions in Australia they are known to attain densities <strong>of</strong> 400-600 kg/ha/annum (de Moor &<br />
Bruton 1988). <strong>Cherax</strong> <strong>tenuimanus</strong> is commercially viable when it reaches a weight <strong>of</strong> 75-125 g (De<br />
Moor 2002). Growth <strong>of</strong> C. <strong>tenuimanus</strong> occurs between 11 and 30°C with 24°C representing optimal<br />
growth temperatures (Morrissy 1990). The life history characteristics (e.g. body size, life span, time<br />
to sexual maturity and reproductive frequency) <strong>of</strong> C. <strong>tenuimanus</strong> could classify it as a k-selected<br />
species (de Moor 2002). Sexual maturity is reached at approximately three years (Picker & Griffiths<br />
2011).<br />
3.3 Reproduction<br />
Records indicate that C. <strong>tenuimanus</strong> breed in spring during their second year <strong>of</strong> life (Safriel & Bruton<br />
1984, de Moor & Bruton 1988). The number <strong>of</strong> eggs produced per individual ranges from 90 to 900<br />
and is dependent on the size <strong>of</strong> the female (Coetzee 1985). Eggs are carried by the female beneath<br />
its tail (pleopods) for a period <strong>of</strong> twelve to sixteen weeks, whereafter they hatch and undergo two<br />
development stages (de Moor & Bruton 1988). After this period, free swimming larvae resembling<br />
the adults are released (de Moor & Bruton 1988). The entire life cycle is completed within<br />
<strong>freshwater</strong> (Cubitt 1985).<br />
3.4 Environmental tolerance ranges<br />
<strong>Cherax</strong> <strong>tenuimanus</strong> is a temperate water species (Read 1985) but will tolerate temperatures as high<br />
as 30°C and as low as 8°C, with adults being more resilient to low temperature (Cubitt 1985). It has<br />
the ability to tolerate salinities <strong>of</strong> up to 18‰ but cannot survive very low oxygen concentrations or<br />
high nutrient conditions (Cubitt 1985). Preferred pH seems to be acidic as they have been cultured at<br />
levels <strong>of</strong> between 5 and 6.5 (Safriel & Bruton 1984). C. <strong>tenuimanus</strong> require good quality water, with<br />
minimal environmental disturbance (TSSC 2005). Similar to other <strong>crayfish</strong> species, it can survive out<br />
<strong>of</strong> water for several days (Ackefors & Lindqvist 1994).<br />
4 History <strong>of</strong> domestication<br />
<strong>Cherax</strong> <strong>tenuimanus</strong> were grown in farm dams in the 1960s in Australia before more serious efforts<br />
were made to improve their growth and survival. This accumulation <strong>of</strong> practical knowledge<br />
represented the beginning <strong>of</strong> the industry. In 1976 legislation was passed to allow the farming <strong>of</strong><br />
marron under strict conditions. This lead to a slow growth <strong>of</strong> the industry until December 1995 when<br />
the industry was producing approximately 18 tons <strong>of</strong> marketable product per annum from a total <strong>of</strong><br />
31 licensed growers. Since the mid to late 1990s, changes made to the legislation have made<br />
commercial farming more attractive. The total production <strong>of</strong> marron in Western Australia for<br />
1999/2000 was above 42 tons from approx 250 licenses (ACWA 2012).<br />
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5 Introduction and spread (South Africa)<br />
C. <strong>tenuimanus</strong> was first introduced into the then Natal province <strong>of</strong> South Africa in 1976 by a private<br />
fish farmer (Borquin et al. 1984). In 1982, the first successfully recorded farm was established in<br />
George (de Moor & Bruton 1988). They were also kept at Pirie hatchery in King Williamstown, where<br />
they managed to escape into the Buffalo River. However, this population did not become established<br />
(Picker & Griffiths 2011). There are anecdotal reports <strong>of</strong> it being found in small streams at Nieu-<br />
Bethesda near Graaff Reinet during the mid 1990s (R. Scott, pers. comm.), and at Madam Dam, near<br />
Stutterheim (de Moor & Bruton 1988), but it is unclear whether these were viably reproducing<br />
populations. It is currently likely to be localised and restricted to a relatively small area in the Eastern<br />
Cape (Figure 3).<br />
Figure 3.<br />
Introduced range (red) <strong>of</strong> C. <strong>tenuimanus</strong> within South Africa (Source: M. Picker & C. Griffiths)<br />
6 Introduction and spread (International)<br />
<strong>Cherax</strong> <strong>tenuimanus</strong> have been experimentally introduced to Louisiana, USA for aquaculture<br />
purposes in the 1970s (Shireman 1973) and into Mauritius in 1990 (FAO 2012). The Food and<br />
Agricultural Organization <strong>of</strong> the United Nations (FAO) claim that the only country <strong>of</strong> introduction is<br />
Mauritius (FAO 2012), however it is unlikely to have established there. Picker & Griffiths (2011)<br />
indicate that it is only known from South Africa and Australia (Figure 2).<br />
7 Compatibility with local environmental conditions<br />
Compatibility <strong>of</strong> this species to local environmental conditions was evaluated by comparing the<br />
ambient annual temperature ranges <strong>of</strong> the 31 terrestrial ecoregions <strong>of</strong> South Africa (Kleynhans et al.<br />
2005) (Figure 4, Table 1) to the known environmental tolerance ranges for C. <strong>tenuimanus</strong> (Cubitt<br />
1985).<br />
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Figure 4. Map <strong>of</strong> South African Ecoregions (Kleynhans et al. 2005).<br />
Page | 5
Table 1.<br />
Altitude and ambient temperature (annual average range and maximum and minimum temperatures<br />
reported) in the 31 ecoregions <strong>of</strong> South Africa. This information was collated from Kleynhans et al.<br />
2005 and assessed to determine compatibility with C. <strong>tenuimanus</strong> culture.<br />
Ecoregion<br />
Altitude (m a.m.s.l)<br />
Temperature<br />
range (°C)<br />
Mean annual<br />
temp (°C)<br />
C. <strong>tenuimanus</strong><br />
climatic suitability<br />
1. Limpopo Plain<br />
300-1100 (1100-1300<br />
limited)<br />
2 to 32 18 to >22 Y<br />
2. Soutpansberg 300-1700 4 to 32 16 to >22 Y<br />
3. Lowveld 0-700; 700-1300 limited 4 to 32 16 to >22 Y<br />
4. North Eastern<br />
300-1300 (1300-1500<br />
Highlands<br />
limited)<br />
2 to 32 16 to 22 Y<br />
5. Northern Plateau<br />
900-1500 (1500-1700<br />
limited)<br />
2 to 30 16 to 20 Y<br />
6. Waterberg<br />
700 –900 (limited), 900-<br />
1700<br />
2 to 32 14 to 22 Y<br />
7. Western Bankenveld 900-1700 0 to 32 14 to 22 Y<br />
8. Bushveld Basin<br />
700-1700 (1700-1900 very<br />
limited)<br />
0 to 32 14 to 22 Y<br />
9. Eastern Bankenveld 500-2300 0 to 30 10 to 22 Y<br />
10. Northern Escarpment 500-900 (limited) 900-<br />
Mountains<br />
2300<br />
0 to 30 10 to 22 Y<br />
11. Highveld<br />
1100-2100, 2100-2300<br />
(very limited)<br />
-2 to 32 12 to 20 Y<br />
12. Lebombo Uplands 0-500 6 to 32 18 to >22 Y<br />
13. Natal Coastal Plain 0-300 8 to 32 20 to >22 Y<br />
14. North Eastern Uplands 0-100 (limited), 100-1500 0 to 30 14 to >22 Y<br />
15. Eastern Escarpment 1100-3100; 3100-3500<br />
Mountains<br />
limited<br />
32 10 to 20 Y<br />
24. South Western<br />
Coastal Belt<br />
0-300; 300-900 limited 4 to 32 10 to 20 Y<br />
25. Western Coastal Belt 0-700, 700-1100 (limited) 2 to >32 16 to 20 Y<br />
26. Nama Karoo<br />
300-1700, 1700-1900<br />
(limited)<br />
0 to >32 12 to 20 Y<br />
27. Namaqua Highlands<br />
100-1300; 1300-1500<br />
limited<br />
2 to 32 12 to 20 Y<br />
28. Orange River Gorge 0-1100 2 to >32 16 to 22 Y<br />
29. Southern Kalahari<br />
500-1700; 1700-1900<br />
limited<br />
-2 to >32 14 to 22 Y<br />
30. Ghaap Plateau 900-1700 0 to 32 16 to 20 Y<br />
31. Eastern Coastal Belt 0-500, 500-900 (limited) 4 to 28 16 to 20 Y<br />
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From this, it is clear that culture <strong>of</strong> C. <strong>tenuimanus</strong> is possible in all but one (the Eastern Escarpment<br />
Mountains) <strong>of</strong> the ecoregions in this country (although some may only be feasible on a seasonal<br />
basis, when the water temperature is above 8°C). Equally, it should be noted that this species is<br />
potentially able to establish naturalised populations in thirty <strong>of</strong> these regions. Indeed, C. <strong>tenuimanus</strong><br />
has reportedly already been introduced and/or is currently established in at least two <strong>of</strong> these<br />
regions (Picker & Griffiths 2011). Established populations have been recorded in the following<br />
regions:<br />
<br />
<br />
16. South Eastern Uplands<br />
31. Eastern Coastal Belt<br />
7.1 Culture techniques<br />
C. <strong>tenuimanus</strong> are typically cultured in earthen ponds. The water in the ponds can be oxygenated<br />
through the use <strong>of</strong> paddlewheel aerators. The ponds require a partial water change every few weeks<br />
to allow a removal <strong>of</strong> sediment and ensure high water quality. Protection from predators (for<br />
example, heron, otters or frogs) is essential, and in South Africa, takes the form <strong>of</strong> pond netting,<br />
corrugated iron fencing and electric fence around the entire facility. In addition, C. <strong>tenuimanus</strong> have<br />
cannibalistic tendencies, so juveniles must be separated from adult populations as soon as possible<br />
(V. Bursey, pers. comm.).<br />
Due to their desiccation tolerance, certain precautions are necessary to reduce the biosecurity risk<br />
(i.e. risk <strong>of</strong> escapement and/or transfer <strong>of</strong> pathogens and diseases to native species). One marron<br />
farm in the Eastern Cape, directs all effluent water into a drainage pond containing predatory fish<br />
such as the largemouth bass (V. Bursey, pers. comm.). Biosecurity risks can be further mitigated<br />
through a range <strong>of</strong> control measures listed in Section 11.<br />
8 Research requirements<br />
Before introducing non-native species, indigenous crustaceans should be considered for aquaculture<br />
wherever possible. However, in order to do so, further research is necessary with regards to the<br />
ecology, genetics, physiology and environmental requirements (Mikkola 1996). As there are no true<br />
<strong>freshwater</strong> <strong>crayfish</strong> native to Africa, other macrocrustaceans, such as crab, should also be<br />
considered for farming.<br />
Currently geographic data on C. <strong>tenuimanus</strong> in South Africa refers only to the distribution <strong>of</strong> the<br />
species and there is no information on abundance at these locations. These data are crucial in<br />
determining the true current and future impacts. In addition, research into the impacts <strong>of</strong> habitat<br />
degradation and climate change on C. <strong>tenuimanus</strong> survival are necessary to determine future<br />
cumulative impacts.<br />
Page | 7
Production (tonnes)<br />
9 Benefit assessment<br />
The Food and Agriculture Organisation <strong>of</strong> the United Nations (FAO) does not publish global annual<br />
statistics on the international production and value <strong>of</strong> C. <strong>tenuimanus</strong>. In 2008, there were two small<br />
marron farms in South Africa (Britz et al. 2009). The total 2010 production from these farms was<br />
listed as was 0.8 tonnes (DAFF 2012a). The growth <strong>of</strong> marron production in South Africa over the last<br />
five years has increased overall (DAFF 2012a) (Figure 5). However, marron farming in South Africa<br />
was valued at less than ZAR 0.1 million in 2008 (Britz et al. 2009).<br />
0.9<br />
0.8<br />
0.7<br />
0.6<br />
0.5<br />
0.4<br />
0.3<br />
0.2<br />
0.1<br />
0<br />
2006 2007 2008 2009 2010<br />
Figure 5.<br />
South African marron production (tonnes) 2006-2010 (Source: DAFF 2012a).<br />
The South African marron farms employed four full time (and no part time) staff in 2008. These<br />
figures are conservative as they include only those involved in primary production and not those<br />
who work in the secondary services (such as feed manufacturers or those employed in fish<br />
processing plants) (Britz et al. 2009).<br />
There are very few <strong>crayfish</strong> aquaculture projects that can be regarded as ‘successful’ (Mikkola 1996).<br />
This, and the recent abandonment <strong>of</strong> a C. quadricarinatus farm in Swaziland, previously touted as<br />
being extremely successful (Copeland 1999), raises serious doubts about the actual benefit <strong>of</strong><br />
culturing <strong>freshwater</strong> <strong>crayfish</strong> species in Africa. On the other hand, there are farms which, despite the<br />
high set up costs and development setbacks, have persevered and are now making a pr<strong>of</strong>it (Burgess<br />
2007).<br />
Schoonbee (1993) also regards the aquaculture potential <strong>of</strong> C. <strong>tenuimanus</strong> as being less favorable<br />
than that <strong>of</strong> the C. destructor-complex and C. quadricarinatus. At least two aquaculture operations<br />
based on C. <strong>tenuimanus</strong>, at Pirie Hatchery near King Williams Town, Eastern Cape, and at Amanzi<br />
farm in the Wilderness area, have been discontinued (de Moor & Bruton 1988). De Moor (2002)<br />
concluded that due to the negative impact <strong>of</strong> introduced parasites that came with C. <strong>tenuimanus</strong>,<br />
and its disappointing results in terms <strong>of</strong> aquaculture, the environmental damage is likely to outweigh<br />
economic benefits.<br />
Currently, export <strong>of</strong> marron to the European Union is under contention, due to the absence <strong>of</strong> a<br />
‘residue monitoring plan’. In addition, China will not allow exports until certain food safety<br />
requirements are met. Export permits are not being issued within South Africa until the relevant<br />
Page | 8
legislation is in place (D. Allan, Kei Chamber <strong>of</strong> Business, pers. comm.). The facilitation <strong>of</strong> exportation<br />
will allow the benefits <strong>of</strong> marron culture to the South African economy to be fully realised.<br />
10 Risk assessment<br />
10.1 Likelihood <strong>of</strong> this species becoming established in South Africa<br />
If introduced for aquaculture purposes, escapes are likely (unless cultured in a closed system), so<br />
such actions should be considered an intentional introduction into the wild which defies the<br />
precautionary principle (Mikkola 1996). C. <strong>tenuimanus</strong> has already escaped from aquaculture<br />
facilities in the Eastern Cape (de Moor & Bruton 1988). Populations, however, failed to establish in<br />
the Buffalo River (de Moor & Bruton 1988). Nevertheless, there are areas <strong>of</strong> the country that have<br />
water bodies with parameters within the tolerance thresholds <strong>of</strong> C. <strong>tenuimanus</strong>. Coetzee (1985)<br />
concluded that the species would be able to survive in the Western Cape should it escape. It is<br />
probable that viable populations <strong>of</strong> C. <strong>tenuimanus</strong> could establish themselves if released into the<br />
upper and mid reaches <strong>of</strong> many perennial rivers where temperatures are moderate and dissolved<br />
oxygen levels sufficient. However, it is unlikely that they would become a significant invasive threat<br />
in all rivers as they are susceptible to predation from otters, water mongoose and cormorants<br />
(Coetzee 1985, de Moor & Bruton 1988).<br />
The invasive potential <strong>of</strong> C. <strong>tenuimanus</strong> in the thirty-one ecoregions <strong>of</strong> South Africa has been<br />
assessed in accordance with the European Non-Native Species Risk Analysis Scheme (ENSARS) (Copp<br />
et al. 2008) developed by CEFAS (UK Centre for Environment, Fisheries & Aquaculture Science).<br />
ENSARS provides a structured framework (Crown Copyright 2007-2008) for evaluating the risks <strong>of</strong><br />
escape, introduction to and establishment in open waters, <strong>of</strong> any non-native aquatic organism being<br />
used (or associated with those used) in aquaculture. For each species, 49 questions are answered,<br />
providing a confidence level and justification (with source listed) for each answer. The questions and<br />
results <strong>of</strong> the assessment on C. <strong>tenuimanus</strong> can be found in Appendix 1.<br />
The outcome <strong>of</strong> the scoring was that C. <strong>tenuimanus</strong> should be further evaluated before additional<br />
introductions are undertaken within South Africa. See Section 12 for tailored recommendations.<br />
10.2 Potential ecological impacts<br />
Escapees from aquaculture facilities are inevitable and occur worldwide, unless appropriate<br />
mitigatory methods are applied. Due to their ability to survive out <strong>of</strong> water and travel across land, C.<br />
<strong>tenuimanus</strong> have the potential to seriously threaten native biodiversity.<br />
The presence <strong>of</strong> alien <strong>crayfish</strong> can lead to dramatic alterations to the benthos which could<br />
potentially indirectly affect fish recruitment and growth (Charlebois & Lamberti 1996). De Moor<br />
(2002) conducted an in depth analysis and review <strong>of</strong> the potential impacts <strong>freshwater</strong> <strong>crayfish</strong> may<br />
have in South Africa. Of the four alien species known to occur in South Africa, C. <strong>tenuimanus</strong> was<br />
deemed to have the least impact (de Moor 2002). Potential impacts caused by C. <strong>tenuimanus</strong> are<br />
likely to be the destruction <strong>of</strong> living aquatic macrophytes, resulting in wide ranging ecosystem<br />
Page | 9
consequences; possible destruction <strong>of</strong> adjacent terrestrial vegetation in the riparian zone, a small<br />
but possible disturbance to breeding <strong>of</strong> bottom-spawning fish, introduction <strong>of</strong> associated<br />
undesirable parasites, and lastly far-ranging but slight impacts on benthic macroinvertebrate<br />
communities the degree to which would be dependent on the population size <strong>of</strong> C. <strong>tenuimanus</strong>. It is<br />
not likely to hybridize with any indigenous species as there are no native <strong>freshwater</strong> African <strong>crayfish</strong><br />
species (de Moor 2002).<br />
The existing broodstock <strong>of</strong> C. <strong>tenuimanus</strong> in South Africa has been used for several years and as a<br />
result, the industry requires new blood lines to prevent inbreeding and associated genetic issues (D.<br />
Impson, Cape Nature, pers. comm.). If new stock is imported from Australia, there are risks <strong>of</strong><br />
disease or parasite introduction which must be considered.<br />
A significant impact which must be considered is the threat <strong>of</strong> introduction <strong>of</strong> undesirable parasites<br />
with C. <strong>tenuimanus</strong>. The ‘<strong>crayfish</strong> plague’ fungus, Aphanomuyces astaci can affect all species in the<br />
Parasticidae family (as well as other <strong>freshwater</strong> <strong>crayfish</strong> families (de Moor 2002). Also, C. <strong>tenuimanus</strong><br />
is host to a microsporidian parasite, Thelohania sp., commonly known as ‘porcelain disease’<br />
(Morrissy et al. 1990, Langdon 1991) which affects striated muscle fibres in the tail. There is no<br />
treatment for this disease and the only means <strong>of</strong> prevention is by ensuring that stocks are disease<br />
free. The disease is, however, extremely difficult to detect during the early stages <strong>of</strong> infection, so the<br />
prevention <strong>of</strong> its importation is likely to be difficult (Langdon 1991). However, this disease has not<br />
been reported in South Africa to date (DAFF 2012b) and is unlikely to affect any indigenous<br />
<strong>freshwater</strong> species.<br />
There are various worms which are hosted by <strong>crayfish</strong> without causing the host any harm, but have<br />
the potential to infect other species. For example, temnocephalan worms (which are non-native to<br />
Africa) can infect other decapods species and can predate on <strong>freshwater</strong> invertebrates.<br />
Temnocephala chaeropsis was introduced with C. <strong>tenuimanus</strong> in the southern Cape and affected the<br />
marketability <strong>of</strong> infected individuals, in some cases causing mortalities (Mitchell & Kok 1988). The<br />
worms also have the potential to infect a species <strong>of</strong> indigenous <strong>freshwater</strong> crab, Potomonautes<br />
warreni (Avenant-Oldewage 1993). In addition, T. chaeropsis could affect other indigenous<br />
decapods, either by killing or by lowering its fitness to a level which would allow C. <strong>tenuimanus</strong> a<br />
competitive advantage (De Moor 2002).<br />
10.3 Potential socio-economic impacts<br />
Currently there are no commercial <strong>freshwater</strong> fisheries in South Africa, and subsistence reliance on<br />
<strong>freshwater</strong> crustaceans is extremely low (B. Clark Anchor Environmental, pers. comm.), so neither <strong>of</strong><br />
these fisheries should suffer significant impacts as a result <strong>of</strong> further introductions <strong>of</strong> C. <strong>tenuimanus</strong>.<br />
It is also unlikely that recreational fisheries will be negatively affected.<br />
Page | 10
10.4 Risk summary<br />
There is reasonable likelihood that:<br />
<br />
<br />
<br />
<br />
<br />
There will be escapees from any established culture facility unless best management<br />
practises are followed;<br />
Unless barriers are provided, C. <strong>tenuimanus</strong> could colonise and establish in any previously<br />
un-invaded streams across some areas <strong>of</strong> the country, especially the Highveld, and in the<br />
southern and south-western Cape (de Moor 2002);<br />
In these areas, introduced <strong>crayfish</strong> will predate on or compete with indigenous species and<br />
will pose a risk (albeit small) to the continued survival <strong>of</strong> native species especially those that<br />
are already rare or range restricted;<br />
No hybridisation will occur with indigenous species; and<br />
Diseases or parasites could be transferred to populations <strong>of</strong> indigenous and non-native<br />
crustaceans unless appropriate best management practises are adopted, and all individuals<br />
are certified disease free by suitably qualified veterinarians prior to introduction.<br />
11 Control and prevention options<br />
There are a number <strong>of</strong> control options for limiting the introduction and spread <strong>of</strong> alien <strong>freshwater</strong><br />
invertebrate species in South Africa. The focus needs to be on preventing their spread or deliberate<br />
introduction to new areas or river systems, as well as seeking to eradicate these animals from<br />
systems where their impact on biodiversity is considered to be unacceptably high.<br />
Controlling the spread <strong>of</strong> invasive species through prevention is thought to be the most costeffective<br />
means (Leung et al. 2002). The <strong>Department</strong> <strong>of</strong> Environmental Affairs & Development<br />
Planning Generic Environmental Best Management Practice Guideline for Aquaculture Development<br />
and Operation in the Western Cape (Hinrichsen 2007) should be used as a guide for construction <strong>of</strong><br />
facilities and management there<strong>of</strong>. These measures can serve to reduce biosecurity risks and key<br />
points from these guidelines are summarised below.<br />
It is recommended that all new aquaculture facilities should be built above the 1 in 50 year flood<br />
line, with infrastructure built to resist the impacts <strong>of</strong> floods (Hinrichsen 2007). The Freshwater<br />
Crayfish policy for the Western Cape Province specifies that facilities must be sited away from<br />
natural waters courses (D. Impson, Cape Nature, pers. comm.).<br />
The creation <strong>of</strong> physical barriers around the facility can also be effective in preventing spread <strong>of</strong><br />
invasive species. Dams have been successful in controlling invasive <strong>freshwater</strong> <strong>crayfish</strong> spread<br />
elsewhere in the world, so may also be a practical consideration for potential marron farms in South<br />
Africa. Three meter high walls were effective in preventing Procambarus clarkii escaping from a<br />
mountain stream in Spain therefore protecting a population <strong>of</strong> the endangered Austropotamobius<br />
pallipes (Dana et al. 2011). Secure fencing around an aquaculture facility in combination with<br />
restricted access will assist in preventing any person intentionally removing live individuals<br />
(Hinrichsen 2007). In addition, facilities must be supervised continuously (i.e. full time) by a person<br />
deemed responsible by the authorities (D. Impson, Cape Nature ,pers. comm.).<br />
Page | 11
In order to decrease the risk <strong>of</strong> escapes, pond culture systems should be designed with stable walls<br />
(free from tree roots or burrowing animals) at a suitable gradient. In the Western Cape, permits will<br />
only be issued if facilities are completely enclosed in ‘’smooth, vertical barriers <strong>of</strong> at least 0.5m high’’<br />
(D. Impson, Cape Nature, pers. comm.). Water levels should be monitored to determine flood<br />
threats and also be built with a capacity for overflow, with an option to be drained completely<br />
(Hinrichsen 2007). Stocking drainage ponds with predatory fish (preferably indigenous species) will<br />
help minimise the risk <strong>of</strong> escapees entering river systems (V. Bursey, pers. comm.). It is<br />
recommended that all outlet and inlet pipes (as well as overflow pipes) should have mesh screens<br />
which will prevent the escape <strong>of</strong> juveniles and adults from the ponds (Hinrichsen 2007, D. Impson,<br />
Cape Nature, pers. comm.).<br />
The creation <strong>of</strong> marron farmers associations in most producing countries has been encouraged and<br />
facilitated (FAO 2012). These associations should encourage their members to adhere to the rules <strong>of</strong><br />
the FAO Code <strong>of</strong> Conduct for Responsible Fisheries and the FAO Technical Guidelines for Responsible<br />
Fisheries (Aquaculture Development). Given that commercial farmers require a licence and must<br />
comply with regulations, they are unlikely to intentionally encourage the spread <strong>of</strong> C. <strong>tenuimanus</strong>.<br />
There are a number <strong>of</strong> control options for limiting the introduction and spread <strong>of</strong> alien <strong>freshwater</strong><br />
species in South Africa. There may be a few potentially small populations <strong>of</strong> C. <strong>tenuimanus</strong> in the<br />
Eastern Cape. The focus thus needs to be on determining if these actually exist or not, determining<br />
what impacts they have had if they do indeed exist, and preventing any further spread. In addition,<br />
the deliberate introduction <strong>of</strong> this species to new areas or river systems should be strictly<br />
prohibited, and remaining populations should be eradicated. Species can be trapped but complete<br />
elimination is considered to be practically impossible (Picker & Griffiths 2011). Assisting and<br />
enhancing high predation rates by indigenous species (e.g. platana frogs, catfish, otters) may be a<br />
possible way to moderate and keep C. <strong>tenuimanus</strong> at low population levels (de Moor 2002). Priority<br />
should be given at the earliest possible stage to contain and eradicate this species (de Moor 2002).<br />
Costs <strong>of</strong> control or eradication and prevention measures should ideally be incurred by the party<br />
responsible for the introduction. However, in practice, this is extremely difficult to determine as well<br />
as enforce (de Moor 2002). Even if there is such an escape pro<strong>of</strong> system there is always the chance<br />
<strong>of</strong> theft as happened to the Australian <strong>freshwater</strong> <strong>crayfish</strong>es which were stolen from a supposedly<br />
escape-pro<strong>of</strong> facility in Bloemfontein (Cambray 2003).<br />
12 Recommendations regarding suitability for use in aquaculture in South Africa<br />
In South Africa, National Freshwater Ecosystem Priority Areas (NFEPA) guidelines provide strategic<br />
spatial priorities for conserving South Africa’s <strong>freshwater</strong> ecosystems and supporting sustainable use<br />
<strong>of</strong> water resources. The NFEPA guidelines were designed to assist those involved in the conservation<br />
and management <strong>of</strong> FEPAs, to preserve these important areas in the high quality condition they<br />
currently exist. FEPAs are river or wetland areas which are in a largely unmodified/natural condition.<br />
These can include free-flowing rivers (free from dam structures), habitats which support threatened<br />
species and their migration corridors, areas which are relied upon as a water source for catchments,<br />
or simply provide a representative selection <strong>of</strong> wetland types. Rivers and their associated subquaternary<br />
catchments which were determined important areas in protecting viable populations <strong>of</strong><br />
threatened and near-threatened fish are broadly termed Fish Sanctuaries.<br />
Page | 12
Figure 6 displays the location <strong>of</strong> FEPAs and their associated sub-quaternary catchments (blue<br />
shading). Fish sanctuaries which are deemed to be <strong>of</strong> high ecological condition were also assigned<br />
FEPA status and accordingly, for the purpose <strong>of</strong> this study, we have grouped together Fish and River<br />
FEPAs. Fish sanctuaries that are not in as good condition but nonetheless recognised as vital to the<br />
protection <strong>of</strong> threatened fish species, were classified as Fish Support Areas (green shading). Fish<br />
migration corridors represent areas for potential migration between essential habitats (yellow<br />
shading). Upstream Management Areas require protection to prevent degradation <strong>of</strong> downstream<br />
areas (brown shading). Phase 2 FEPA sub- quaternary catchments (pink shading) include riverine<br />
areas that are in a poorer ecological condition but nonetheless still considered important for<br />
conservation <strong>of</strong> <strong>freshwater</strong> aquatic resources provided they can be rehabilitated. Rehabilitation <strong>of</strong><br />
these areas is expected to be undertaken when all other FEPAS are considered well managed.<br />
Collectively, these areas all represent important habitats and sites for the conservation <strong>of</strong> <strong>freshwater</strong><br />
biodiversity in South Africa and should be protected from development and other adverse impacts.<br />
In spite <strong>of</strong> their value in conservation planning and management, FEPAs are considered to be <strong>of</strong><br />
lesser value in guiding decision making regarding allocation <strong>of</strong> aquaculture permits for alien species<br />
such as C. <strong>tenuimanus</strong>. This is because FEPAs tend to cover restricted conservation worthy aquatic<br />
ecosystems within river basins or sub-quaternary catchments that are by nature, linked to the rest <strong>of</strong><br />
the catchment by existing river channels. C. <strong>tenuimanus</strong>, being mostly highly mobile, can very easily<br />
invade an area designated as a FEPA from virtually any other portion <strong>of</strong> the catchment except where<br />
a barrier (such as a dam wall or waterfall) prevents this from happening. In addition, FEPA maps do<br />
not <strong>of</strong>fer a species-specific approach i.e. the FEPAs recommend that no species be farmed in these<br />
areas. However, not all species will impact on threatened native species in an equal manner.<br />
For this reason a complimentary mapping process (termed the NEM:BA AIS fish maps, Swartz 2012)<br />
was initiated specifically to support the process <strong>of</strong> identifying locations for the farming <strong>of</strong> alien<br />
invasive <strong>freshwater</strong> fish species. These maps are based on the same sub-quaternary layers as utilised<br />
in the FEPA process, and are thus compatible with the NFEPA maps. Biodiversity protection was<br />
maximised wherever possible in both sets <strong>of</strong> maps, however, no consideration was given to climatic<br />
suitability for the non-indigenous species <strong>of</strong> concern. The NEM:BA maps were created using known<br />
distribution records and expert opinion. These maps were then developed in consultation with<br />
anglers and aquaculturists to take into account socio-economic impacts <strong>of</strong> the zonation process (O.<br />
Weyl, SAIAB, pers. comm.).<br />
A NEM:BA AIS fish map has been prepared for C. <strong>tenuimanus</strong> on the premise that C. <strong>tenuimanus</strong> is a<br />
NEM:BA List 3: Category 2 species i.e. one to be managed by area. Category 2 species generally have<br />
high economic value for aquaculture and angling, but have a high potential negative impact on the<br />
environment where they occur outside their native range. In the case <strong>of</strong> C. <strong>tenuimanus</strong>, it is classed<br />
as a species with no risk <strong>of</strong> hybridisation or genetic contamination.<br />
These maps have not been implemented by government as part <strong>of</strong> the legislative regime as yet,<br />
owing to the fact that NEM:BA currently does not allow for the approach <strong>of</strong> regulating these species<br />
as envisaged by the maps. As a result, they have not been included in this Biodiversity Risk and<br />
Benefit Assessment pr<strong>of</strong>ile.<br />
Page | 13
Figure 6. South Africa’s Ecoregions with FEPAs, Fish Support areas, Fish Corridors, Upstream Management Areas and Phase 2 FEPAs. Source: Kleynhans et al. 2005 and Nel 2011.<br />
Page | 14
It is recommended that conservation authorities responsible for evaluating aquaculture permit<br />
applications should make use <strong>of</strong> all <strong>of</strong> the available resources including the FEPA maps and<br />
ecoregions maps as well as the NEM:BA AIS fish maps when these are released, to inform their<br />
decision making processes. However, this remains a complex procedure, despite the availability <strong>of</strong><br />
these visual tools, therefore further consultation with experts may be necessary.<br />
At present, in the absence <strong>of</strong> the NEM:BA AIS maps, recommendations for culture activities have<br />
been based on the FEPA maps (Figure 6) and environmental tolerance ranges <strong>of</strong> the species (Table<br />
1). In the first instance, it is recommended that no permits for culture activities be issued in areas<br />
designated as FEPAs (Table 2). All aquaculture facilities in Phase 2 FEPAs, should have high<br />
biosecurity measures in place, in order to protect non-fish species which are threatened and may<br />
not be directly protected in the FEPAs or Fish Support Areas. In the case <strong>of</strong> marron, this involves<br />
either closed Recirculating Aquaculture Systems (RAS) or a facility that is constructed at a distance <strong>of</strong><br />
greater than 1000 m from a watercourse.<br />
Table 2.<br />
Recommendations for C, <strong>tenuimanus</strong> culture in South Africa. Red shading indicates ‘No culture”, orange<br />
shading indicates “high biosecurity” (closed RAS or facilities >1000 m from watercourse), blue shading<br />
indicates “medium biosecurity” (partial RAS or pond >500 m from watercourse) and green shading indicates<br />
low biosecurity requirements (pond >100 m from a watercourse). White blocks represent “Nonapplicability”,<br />
i.e. in this case, there is no native distribution <strong>of</strong> C. <strong>tenuimanus</strong> in South Africa. ‘1’ has been<br />
categorised as high biosecurity to include the protection <strong>of</strong> non-fish threatened species (which are not<br />
directly recognised in the fish sanctuary format <strong>of</strong> FEPAs).<br />
FEPA map category<br />
Native<br />
distribution<br />
Existing<br />
introduced<br />
population<br />
Species not<br />
present<br />
(climatically<br />
suitable)<br />
Species not<br />
present<br />
(climatically<br />
unsuitable)<br />
FEPA (Fish and River FEPAs)<br />
Fish Support Area<br />
Fish Corridor<br />
Upstream management Area<br />
Phase 2 FEPAs 1 1 1<br />
All other areas<br />
In Fish Support Areas, where the species is currently not present, (whether the climate is suitable for<br />
culture or not), culture <strong>of</strong> C. <strong>tenuimanus</strong> can be undertaken only following construction <strong>of</strong> the high<br />
biosecurity facilities. In Fish Support Areas, where the species is currently found, culture should only<br />
be permitted with medium biosecurity measures in place (i.e. partial RAS or in a facility that has<br />
been constructed at a distance <strong>of</strong> greater than 500 m from a watercourse).<br />
Culture activities in Fish Corridors and Upstream Management Areas should be restricted to high<br />
biosecurity facilities in water catchments which are suitable for culture but the species is currently<br />
not present. If the species is already established in these areas, culture facilities must be <strong>of</strong> a<br />
medium biosecurity status. Where marron is not present and the area is climatically unsuitable, low<br />
biosecurity measures can be employed (where the pond is sited at a distance <strong>of</strong> greater than 100 m<br />
from a watercourse).<br />
Page | 15
In all other <strong>freshwater</strong> areas, low biosecurity culture facilities can be installed. It should be noted<br />
that all these recommended levels <strong>of</strong> biosecurity should be implemented in conjunction with the<br />
other preventative measures discussed in Section 11.<br />
The construction <strong>of</strong> closed and partial recirculating facilities which treat water and/or recyclewater<br />
should be encouraged wherever possible, to prevent the discharge <strong>of</strong> organisms and waste products<br />
into the surrounding environment.<br />
De Moor (2002) warns against the further import <strong>of</strong> live C. <strong>tenuimanus</strong>. He concludes that due to the<br />
high risk posed by parasites <strong>of</strong> C. <strong>tenuimanus</strong> infecting indigenous species, and the damages already<br />
caused by this, combined with the disappointing results that have been achieved so far in terms <strong>of</strong><br />
aquaculture, it is clear the environmental damage has already outweighed the economic benefits<br />
from imports, and that there is no point in allowing the importation <strong>of</strong> species unlikely to be <strong>of</strong><br />
commercial value.<br />
An assessment <strong>of</strong> the invasive potential <strong>of</strong> C. <strong>tenuimanus</strong> undertaken in accordance with the<br />
European Non-Native Species Risk Analysis Scheme (ENSARS) suggests that C. <strong>tenuimanus</strong> should be<br />
evaluated in more detail before further introduced to this country. Assessments conducted on the<br />
potential impacts <strong>of</strong> this species on local fauna in areas where it has already been introduced<br />
indicate that these impacts are potentially severe, especially the threat posed by diseases and<br />
parasites. Scientific monitoring should be undertaken at the current aquaculture facilities to assess<br />
the impacts <strong>of</strong> individual farms (and the techniques utilised in each facility).<br />
13 References<br />
Ackefors, H. &. Lindqvist, O.V. 1994. Cultivation <strong>of</strong> <strong>freshwater</strong> <strong>crayfish</strong>es in Europe. In: Huner, J.V.<br />
(Ed.) Freshwater <strong>crayfish</strong> aquaculture in North America, Europe, and Australia. Food<br />
Products Press, New York, USA. Pp.157-216.<br />
ACWA. 2012. Aquaculture Council <strong>of</strong> Western Australia.<br />
http://www.aquaculturecouncilwa.com/marron/guide-to-marron-farming Accessed: 10<br />
September 2012.<br />
Austin, C.M. & Ryan, S.G. 2002. Allozyme evidence for a new species <strong>of</strong> <strong>freshwater</strong> <strong>crayfish</strong> <strong>of</strong> the<br />
genus <strong>Cherax</strong> Erichson (Decapoda: Parastacidae) from the south-west <strong>of</strong> Western Australia.<br />
Invertebrate Systematics 16: 357-367.<br />
Austin, C.M. & Bunn, J. 2010. <strong>Cherax</strong> <strong>tenuimanus</strong>. In: IUCN 2012. IUCN Red List <strong>of</strong> Threatened<br />
Species. Version 2012.1. . Downloaded on 22 August 2012.<br />
Avenant-Oldewage, A. 1993. Occurrence <strong>of</strong> Temnocephala chaeropsis on <strong>Cherax</strong> <strong>tenuimanus</strong><br />
imported into South Africa, and notes on its infestation <strong>of</strong> an indigenous crab. South African<br />
Journal <strong>of</strong> Science 89: 427–428.<br />
Borquin, O., Pike, T., Johnson, D., Rowe-Rowe, D. & Appleton, C.C. 1984. Alien animal species.<br />
Internal report to the Natal Parks, Game and Fish Preservation Board, Pietermartizburg. Pp.<br />
36.<br />
Britz, P.J., Lee, B. & Botes, L. 2009. AISA 2009 Aquaculture Benchmarking Survey: Primary Production<br />
and Markets. AISA report produced by Enviro-Fish Africa (Pty) Ltd. 117 pp.<br />
Page | 16
Burgess, M. 2007. Pioneers <strong>of</strong> SA marron production. Farmer's Weekly Magazine. Mon 30 April 2007.<br />
http://www.farmersweekly.co.za/article.aspxid=520&h=Pioneers-<strong>of</strong>-SA-marron-production<br />
Cambray, J.A. 2003. Impact on indigenous species biodiversity caused by the globalization <strong>of</strong> alien<br />
recreational <strong>freshwater</strong> fisheries. Hydrobiologia 500: 217–230.<br />
Coetzee, D.J. 1985. Verslag oor die kunsmatige aanhouding van die marron, <strong>Cherax</strong> <strong>tenuimanus</strong>, by<br />
die Jonkershoek-Natuurbewaringstasie, Stellenbosch. Internal Report to the Director <strong>of</strong> the<br />
Cape <strong>Department</strong> <strong>of</strong> Nature and Environmental Conservation.<br />
Charlebois, P.M. & Lamberti G.A. 1996. Invading <strong>crayfish</strong> in a Michigan stream: direct and indirect<br />
effects on periphyton and macroinvertebrates. Journal <strong>of</strong> the North American Benthological<br />
Society 15: 551-563.<br />
Copeland, J. 1999. Rich pickings from <strong>crayfish</strong>. Farmer’s Weekly, 8 October 1999: 89–91.<br />
Copp, G.H., Britton, J.R., Cowx, I.G., Jeney, G., Joly, J-P., Gherardi, F., Gollasch, S., Gozlan, R.E., Jones,<br />
G., MacLeod, A., Midtlyng, P.J., Miossec, L., Nunn, A.D., Occhipinti-Ambrogi, A., Oidtmann,<br />
B., Olenin, S., Peeler, E., Russell, I.C., Savini, D., Tricarico, E. & Thrush, M. 2008. Risk<br />
assessment protocols and decision making tools for use <strong>of</strong> alien species in aquaculture and<br />
stock enhancement. EU Co-ordination Action Project: IMPASSE Environmental impacts <strong>of</strong><br />
alien species in aquaculture, Deliverable report 3.2.<br />
Cubitt, G.H. 1985. Candidate species in aquaculture: <strong>freshwater</strong> <strong>crayfish</strong>. In: Hecht, T., Bruton, M.N.<br />
& Safriel, O. (Eds.). Aquaculture South Africa. Occasional Report Series No. 1. Foundation for<br />
Research and Development, CSIR. Pp. 30-32.<br />
DAFF 2012a. <strong>Department</strong> <strong>of</strong> Agriculture, Forestry and Fisheries. South Africa’s Auaculture Annual<br />
Report 2011.<br />
DAFF 2012b. <strong>Department</strong> <strong>of</strong> Agriculture, Forestry and Fisheries. Animal Disease Status <strong>of</strong> South<br />
Africa (24 February 2012).<br />
Dana, E.D., Garcia-de-Lomasa, J., Gonzaleza, R. & Ortega, F. 2011. Effectiveness <strong>of</strong> dam construction<br />
to contain the invasive <strong>crayfish</strong> Procambarus clarkii in a Mediterranean mountain stream.<br />
Ecological Engineering 37: 1607–1613.<br />
de Moor, I.J. 2002. Potential impacts <strong>of</strong> alien <strong>freshwater</strong> <strong>crayfish</strong> in South Africa. African Journal <strong>of</strong><br />
Aquatic Science 27: 125-139.<br />
de Moor, I.J. & Bruton, M.N. 1988. Atlas <strong>of</strong> alien and translocated indigenous aquatic animals in<br />
southern Africa. South African National Scientific Programmes Report No. 144. CSIR.<br />
Driver, A., Nel, J.L., Snaddon, K., Murray, K., Roux, D., Hill, L., Swartz, E.R., Manuel, J. & Funke, N.<br />
2011. Implementation Manual for Freshwater Ecosystem Priority Areas. WRC Report No.<br />
1801/1/11. ISBN 978-1-4312-0147-1. Pretoria.<br />
FAO. 2012. http://www.fao.org/fishery/introsp/294/en Accessed: 10 September 2012.<br />
Hinrichsen, E. 2007. Generic Environmental Best Practice Guideline for Aquaculture Development<br />
and Operation in the Western Cape: Edition 1. Division <strong>of</strong> Aquaculture, Stellenbosch<br />
University Report. Republic <strong>of</strong> South Africa, Provincial Government <strong>of</strong> the Western Cape,<br />
<strong>Department</strong> <strong>of</strong> Environmental Affairs & Development Planning, Cape Town.<br />
Kleynhans, C.J., Thirion, C. & Moolman, J. 2005. A Level I River Ecoregion classification System for<br />
South Africa, Lesotho and Swaziland. Report No. N/0000/00/REQ0104. Resource Quality<br />
Services, <strong>Department</strong> <strong>of</strong> Water Affairs and Forestry, Pretoria, South Africa.<br />
Langdon, J.S. 1991. Microsporidiosis due to a plesstophorid in marron, <strong>Cherax</strong> <strong>tenuimanus</strong> (Smith),<br />
(Decapoda: Parastacidae). Journal <strong>of</strong> Fish Diseases 14: 33–44.<br />
Leung, B., Lodge, D.M., Finn<strong>of</strong>f, D., Shogren, J.F., Lewis, M.A. & Lamberti, G. 2002. An ounce <strong>of</strong><br />
Page | 17
prevention or a pound <strong>of</strong> cure: bioeconomic risk analysis <strong>of</strong> invasive species. Proceedings <strong>of</strong><br />
the Royal Society <strong>of</strong> London B 269: 2407-2413.<br />
Mikkola, H. 1996. Alien <strong>freshwater</strong> crustacean and indigenous mollusc species with aquaculture<br />
potential in eastern and southern Africa. Southern African Journal <strong>of</strong> Aquatic Sciences 22:<br />
90-99.<br />
Mitchell, S.A. & Kok, D.C. 1988. Alien symbionts introduced with imported marron from Australia<br />
may pose a threat to aquaculture. South African Journal <strong>of</strong> Science 84: 877–878.<br />
Morrissy, N.M. 1990. Optimum and favourable temperatures for growth <strong>of</strong> <strong>Cherax</strong> <strong>tenuimanus</strong><br />
(Smith 1912) (Decapoda: Parastacidae). Australian Journal <strong>of</strong> Marine and Freshwater<br />
Research 41: 735–746.<br />
Picker, M.D. & Griffiths, C.L. 2011. Alien and Invasive Animals – A South African Perspective.<br />
Randomhouse/Struik Cape Town. 240pp<br />
Read, G.H.L. 1985. A possible aquacultural crustacean with temperate growth requirements. In:<br />
Aquaculture South Africa. Proceedings <strong>of</strong> a joint symposium by the CSIR and the South<br />
African Agricultural Union. Occasional report no. 1 30-32.<br />
Safriel, O. & Bruton, M.N. 1984. A cooperative aquaculture research programme for South Africa.<br />
South African National Scientific Programmes Report 89. CSIR, Pretori pp. 79.<br />
Schoonbee, H.J. 1993. Report to the Chief Directorate: Nature and Environmental Conservation <strong>of</strong><br />
the Transvaal on the Australian <strong>freshwater</strong> <strong>crayfish</strong> <strong>Cherax</strong> albidus (yabbiee) and C.<br />
quadricarinatus (red claw). Unpublished report, Zoology <strong>Department</strong>, Rand Afrikaans<br />
University, South Africa, 64pp.<br />
Shireman, J.V. 1973. Experimental introduction <strong>of</strong> the Australian <strong>crayfish</strong> (<strong>Cherax</strong> <strong>tenuimanus</strong>) into<br />
Louisiana. The Progressive Fish-Culturist 35: 107-109.<br />
Swartz, E. 2012. Summary <strong>of</strong> the mapping process for alien invasive fishes for NEM:BA (list 3<br />
category 2: species managed by area). Prepared for the South African National Biodiversity<br />
Institute.<br />
TSSC 2005. Advice to the Minister for the Environment and Heritage from the Threatened Species<br />
Scientific Committee (the Committee) on Amendments to the list <strong>of</strong> Threatened Species<br />
under the Environment Protection and Biodiversity Conservation Act 1999 (EPBC Act) –<br />
<strong>Cherax</strong> <strong>tenuimanus</strong>.<br />
Page | 18
Appendix 1. Risk scoring methodology for C. <strong>tenuimanus</strong> for South Africa (all ecoregions) with guidance supplied by the FI-ISK<br />
toolkit (Copp et al. 2008).<br />
Risk query:<br />
Question Biogeography/historical Reply Comments & References Certainty<br />
1<br />
Is the species adapted for aquacultural or ornamental purposes Guidance: This taxon must have been grown<br />
deliberately and subjected to substantial human selection for at least 20 generations, or is known to be easily reared in<br />
captivity (e.g. aquaculture or aquaria).<br />
Y<br />
ACWA 2012; Picker & Griffiths<br />
2011 4<br />
2<br />
Has the species become naturalised where introduced Guidance: The taxon must be known to have successfully<br />
established self-sustaining populations in at least one habitat other than its usual habitat (eg. Lotic vs lentic) and<br />
persisted for at least 50 years (response modifies the effect <strong>of</strong> Q1). Y Picker & Griffiths 2011 3<br />
3<br />
Does the species have invasive races/varieties/sub-species Guidance: This question emphasizes the invasiveness <strong>of</strong><br />
domesticated, in particular ornamental, species (modifies the effect <strong>of</strong> Q1). Y TSSC 2005 4<br />
4<br />
Is species reproductive tolerance suited to climates in the risk assessment area (1-low, 2-intermediate, 3-high) )<br />
Guidance: Climate matching is based on an approved system such as GARP or Climatch. If not available, then assign the<br />
maximum score (2). 2 Cubitt 1985 4<br />
5<br />
What is the quality <strong>of</strong> the climate match data (1-low; 2-intermediate; 3-high) ) Guidance: The quality is an estimate<br />
<strong>of</strong> how complete are the data used to generate the climate analysis. If not available, then the minimum score (0)<br />
should be assigned. 2 Kleynhans et al. 2005 4<br />
6<br />
Does the species have broad climate suitability (environmental versatility) Guidance: Output from climate matching<br />
can help answer this, combined with the known versatility <strong>of</strong> the taxon as regards climate region distribution.<br />
Otherwise the response should be based on natural occurrence in 3 or more distinct climate categories, as defined by<br />
Koppen or Walter (or based on knowledge <strong>of</strong> existing presence in areas <strong>of</strong> similar climate). N Cubitt 1985 3<br />
7<br />
Is the species native to, or naturalised in, regions with equable climates to the risk assessment area Guidance:<br />
Output from climate matching help answer this, but in absence <strong>of</strong> this, the known climate distribution (e.g. a tropical,<br />
semi-tropical, south temperate, north temperate) <strong>of</strong> the taxons native range and the ‘risk are’ (,e, country/region/area<br />
for which the FISK is being run) can be used as a surrogate means <strong>of</strong> estimating. Y Picker & Griffiths 2011 3<br />
8<br />
Does the species have a history <strong>of</strong> introductions outside its natural range Guidance: Should be relatively well<br />
documented, with evidence <strong>of</strong> translocation and introduction. N de Moor & Bruton 1985 3<br />
9<br />
Has the species naturalised (established viable populations) beyond its native range Guidance: If the native range is<br />
not well defined (i.e. uncertainty about it exists), or the current distribution <strong>of</strong> the organism is poorly documented,<br />
then the answer is “Don’t know”. Y de Moor & Bruton 1985 4<br />
10<br />
In the species' naturalised range, are there impacts to wild stocks <strong>of</strong> angling or commercial species Guidance:<br />
Where possible, this should be assessed using documented evidence <strong>of</strong> real impacts (i.e. decline <strong>of</strong> native species,<br />
disease introduction or transmission), not just circumstantial or opinion-based judgments. N No record <strong>of</strong> this 3<br />
11<br />
In the species' naturalised range, are there impacts to aquacultural, aquarium or ornamental species Guidance:<br />
Aquaculture incurs a cost from control <strong>of</strong> the species or productivity losses. This carries more weight than Q10. If the N de Moor 2002 3<br />
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12<br />
13<br />
14<br />
15<br />
16<br />
17<br />
18<br />
19<br />
20<br />
21<br />
22<br />
23<br />
24<br />
25<br />
types <strong>of</strong> species is uncertain, then the yes response should be placed here for more major species, particularly if the<br />
distribution is widespread.<br />
In the species' naturalised range, are there impacts to estuaries, coastal waters or amenity values Guidance:<br />
documented evidence that the species has altered the structure or function <strong>of</strong> natural ecosystems. PLEASE NOTE THAT<br />
THIS IS AN ERROR WITH THE FIISK TOOLKIT AND THE CREATORS WILL BE ALERTED. FOR THE PURPOSES OF THIS STUDY,<br />
THE QUESTION SHOULD BE “In the species' naturalised range, are there impacts to rivers, lakes or amenity values” N No record <strong>of</strong> this 3<br />
Does the species have invasive congeners Guidance: One or more species within the genus are known to be serious<br />
pests. N GISD 2012 4<br />
Is the species poisonous, or poses other risks to human health Guidance: Applicable if the taxon’s presence is<br />
known, for any reason, to cause discomfort or pain to animals. In the case <strong>of</strong> mollusks, which can become poisonous<br />
to humans by accumulating algae toxins, restrict this question to animals other than humans. N No record <strong>of</strong> this 4<br />
Does the species out-compete with native species Guidance: known to suppress the growth <strong>of</strong> native species, or<br />
displace from the microhabitat, <strong>of</strong> native species. Y de Moor 2002 3<br />
Is the species parasitic <strong>of</strong> other species or may it act a major predator on a native species that was previously<br />
subject to low predation Guidance: Needs at least some documentation <strong>of</strong> being a parasite <strong>of</strong> other species N No reference 3<br />
Is the species unpalatable to, or lacking, natural predators Guidance: this should be considered with respect to<br />
where the taxon is likely to be present and with respect to the likely level <strong>of</strong> ambient natural or human predation, if<br />
any. N No reference 4<br />
Is the species likely to exert a notable increased predation on any native species Guidance: There should be<br />
evidence that the species is known to reduce the abundance <strong>of</strong> native species. Y de Moor 2002 3<br />
Does the species host, and/or is it a vector, for recognised pests and pathogens, especially non-native Guidance:<br />
The main concerns are non-native pathogens and parasites, with the host being the original introduction vector <strong>of</strong> the<br />
Mitchell & Kock 1988; Avenantdisease<br />
or as a host <strong>of</strong> the disease brought in by another taxon.<br />
Y Oldewage 1993 4<br />
For crustaceans, does the species achieve an ultimately large body size (e.g > 10 cm body length) or for mussels,<br />
does the species form extensive colonies/cluster/aggregations (e.g. >1m^3) ) Guidance: Although small-bodied<br />
invertebrates may be abandoned, large-bodied invertebrates are the major concern, as they soon outgrow their<br />
aquarium. Y Picker & Griffiths 2011 4<br />
Does the species tolerate a wide range <strong>of</strong> salinity regimes Guidance: There should be evidence that the species<br />
tolerates a wide range <strong>of</strong> salinities, from <strong>freshwater</strong> to highly saline. N Cubitt 1985 4<br />
Is the species desiccation tolerant at some stage <strong>of</strong> its life cycle Guidance: Should be able to withstand being out <strong>of</strong><br />
water for extended periods (e.g. minimum <strong>of</strong> one or more hours). Y Ackefors & Lindqvist 1994 4<br />
Is the species flexible/versatile in terms <strong>of</strong> habitat use Guidance: Species that are known to persist in a wide variety<br />
<strong>of</strong> habitats, including areas <strong>of</strong> standing and flowing waters (over a wide range <strong>of</strong> Velocities: 0 to 0.7 m per sec). Y TSSC 2005 3<br />
Does feeding, settlement or other behaviours <strong>of</strong> the species reduce habitat quality for native species Guidance:<br />
There should be evidence that the foraging results in an increase in suspended solids, reducing water clarity water<br />
chemistry etc. Y de Moor 2002 3<br />
Does the species require minimum population size to maintain a viable population Guidance: If evidence <strong>of</strong> a<br />
population crash or extirpation due to low numbers (e.g. overexploitation, pollution, etc.), then response should be<br />
‘yes’.<br />
Y<br />
Need certain number to<br />
prevent inbreeding 4<br />
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26<br />
27<br />
28<br />
Does the species have a wide temperature tolerance range Guidance: There should be documented evidence <strong>of</strong> the<br />
taxon being able to survive in extreme low and/or high temperatures. N V. Bursey pers. comm. 4<br />
Is the species a voracious predator Guidance: Obligate piscivores are most likely to score here, but some facultative<br />
species may become voracious when confronted with naïve prey. N No record <strong>of</strong> this 3<br />
Is the species omnivorous Guidance:Evidence exists <strong>of</strong> foraging on a wide range <strong>of</strong> prey items, including incidental<br />
piscivory. Y Read 1985 4<br />
29 Is the species planktivorous or detrivorous Guidance: Should be an obligate planktivore to score here. Y Read 1985 4<br />
30<br />
Does it exhibit parental care and/or is it known to reduce age-at-maturity in response to environment Guidance:<br />
Needs at least some documentation <strong>of</strong> expressing parental care and/or variable age at maturity under different<br />
environmental conditions. Y de Moor & Bruton 1988 4<br />
31 Does the species produce viable gametes Guidance: If the taxon is a sub-species, then it must be indisputably sterile. Y No reference 4<br />
32<br />
Does the species hybridize naturally with native species Guidance: Documented evidence exists <strong>of</strong> interspecific<br />
hybrids occurring, without assistance under natural conditions. N No reference 4<br />
33<br />
Is the species hermaphroditic or gynogenetic (e.g. Melanoides tubercolata or the marble <strong>crayfish</strong>) Guidance: Needs<br />
at least some documentation <strong>of</strong> hermaphroditism or gynogenesis. N de Moor & Bruton 1988 4<br />
34<br />
Is the species dependent on the presence <strong>of</strong> another species or specific habitat features to complete life cycle<br />
Guidance: Some species may require specialist incubators (e.g. unionid mussels used by bitterling) or specific habitat<br />
features (e.g. fast flowing water, particular species <strong>of</strong> plant or types <strong>of</strong> substrata) in order to reproduce successfully. N No record <strong>of</strong> this 4<br />
35<br />
Is the species highly fecund, iteropatric or extended spawning season Guidance: Species is considered to have<br />
relatively high fecundity for its taxonomic Order. N Coetzee 1995 4<br />
36<br />
What is the species' known minimum generation time (in years) Guidance: Time from hatching to full maturity (i.e.<br />
active reproduction, not just presence <strong>of</strong> gonads). Please specify the number <strong>of</strong> years. 3 Picker & Griffiths 2011 4<br />
37<br />
Are life stages likely to be dispersed unintentionally Guidance: Unintentional dispersal resulting from human<br />
activity, including as ship ballast or hull foulant. PLEASE NOTE THAT THIS IS AN ERROR WITH THE FIISK TOOLKIT AND<br />
THE CREATORS WILL BE ALERTED. FOR THE PURPOSES OF THIS STUDY, THE GUIDANCE SHOULD BE Unintentional<br />
dispersal resulting from human activity. Y Mikkola 1996 3<br />
38 Are life stages likely to be dispersed intentionally by humans (and suitable habitats abundant nearby) N No record <strong>of</strong> this 3<br />
39<br />
40<br />
41<br />
Are life stages likely to be dispersed as a contaminant <strong>of</strong> commodities Guidance: Taxon is associated with organisms<br />
likely to be sold commercially.<br />
N<br />
Depends on management<br />
practices 3<br />
Does natural dispersal occur as a function <strong>of</strong> dispersal <strong>of</strong> eggs and/or the movement <strong>of</strong> the suitable substratum<br />
Guidance: there should be documented evidence that eggs are taken by water currents or displaced by other<br />
organisms either intentionally or not. N de Moor & Bruton 1988 4<br />
Does natural dispersal occur as a function <strong>of</strong> larval or juvenile dispersal (along linear and 'stepping stone' habitats)<br />
Guidance: There should be documented evidence that larvae enter, or are taken by, water currents, or can move<br />
between marine areas via connections. PLEASE NOTE THAT THIS IS AN ERROR WITH THE FIISK TOOLKIT AND THE<br />
CREATORS WILL BE ALERTED. FOR THE PURPOSES OF THIS STUDY, THE GUIDANCE SHOULD BE: There should be<br />
documented evidence that larvae enter, or are taken by, water currents, or can move between water bodies via<br />
connections Y de Moor & Bruton 1988 4<br />
42 Are adults <strong>of</strong> the species known to migrate (reproduction, feeding, etc.) Guidance: There should be documented Y Molony et al. 2003 4<br />
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43<br />
44<br />
45<br />
46<br />
47<br />
48<br />
49<br />
evidence <strong>of</strong> migratory behavior, even at a small scale (tens or hundreds <strong>of</strong> meters).<br />
Are any life stages <strong>of</strong> the species known to be dispersed by other animals (externally) Guidance: For example, are<br />
they moved by birds accidentally when the water fowl move from one marine area to another For example, are they<br />
moved by birds accidentally when the water fowl move from one water body to another No record <strong>of</strong> this 2<br />
Is dispersal <strong>of</strong> the species density dependent Guidance: There should be documented evidence <strong>of</strong> the taxon<br />
spreading out or dispersing when its population density increases. N No record <strong>of</strong> this 3<br />
Is any life history stage likely to survive out <strong>of</strong> water transport Guidance: There should be documented evidence <strong>of</strong><br />
the taxon being able to survive for an extended period (e.g. an hour or more) out <strong>of</strong> water. PLEASE NOTE THAT THIS IS<br />
SIMILAR TO QUESTION 22. THIS IS AN ERROR WITH THE FIISK TOOLKIT AND THE CREATORS WILL BE ALERTED. FOR THE<br />
PURPOSES OF THIS STUDY, THE ANSWER HAS BEEN REPEATED. Y Ackefors & Lindqvist 1994 4<br />
Does the species tolerate a wide range <strong>of</strong> water quality conditions, especially oxygen depletion & high<br />
temperature Guidance: This is to identify taxa that can persist in cases <strong>of</strong> low oxygen and elevated levels <strong>of</strong> naturally<br />
occurring chemicals (e.g. ammonia). N Cubitt 1985 4<br />
Is the species susceptible to chemical control agents Guidance: There should be documented evidence <strong>of</strong><br />
susceptibility <strong>of</strong> the taxon to chemical control agents. No record <strong>of</strong> this 1<br />
Does the species tolerate or benefit from environmental disturbance Guidance: The growth and spread <strong>of</strong> some<br />
taxa may be enhanced by disruptions or unusual events (coastal turbidity due to river floods and/or spates), especially<br />
human impacts (coastal dredging, desiccation, trawl fishing, etc). PLEASE NOTE THAT THIS IS AN ERROR WITH THE FIISK<br />
TOOLKIT AND THE CREATORS WILL BE ALERTED. FOR THE PURPOSES OF THIS STUDY, THE GUIDANCE SHOULD BE: The<br />
growth and spread <strong>of</strong> some taxa may be enhanced by disruptions or unusual events (floods, spates, dessication),<br />
especially human impacts. N TSSC 2005 4<br />
Does the species have effective natural enemies present in the risk assessment area Guidance: A known effective<br />
natural enemy <strong>of</strong> the taxon may or may not be present in the Risk Assessment area. The answer is ‘Don’t know’ unless<br />
a specific enemy/enemies is known. Y Picker & Griffiths 2011 4<br />
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