Method for producing conidia through artificially inducing Mycocentrospora Acerina

[0043] Example 1: The experimenters collected the diseased leaves of Panax notoginseng round spot disease in Qiubei County, Wenshan Prefecture, Yunnan Province in July 2019, and isolated the pathogenic bacteria by tissue separation method. After culturing the purified pathogenic strain SDM5 on PDA medium for 7 days , cut a square piece of mycelium with a scalpel and put in sterile ddH 2The induction was carried out in O, and after 15 days of alternating light and dark culture in a light incubator at 20°C, sporulation began, and then the amount of sporulation increased.

[0044] Example 2: In September 2019, the experimenters collected the diseased leaves of Panax notoginseng round spot in the Linxia Panax notoginseng base of Lancang County, Puer City, isolated and purified the pathogenic strain DLC16, and used the same method as in Example 1 to cultivate Conidia began to be produced after 10 days.

[0045] The present invention proposes a new conidia induction method aimed at the pathogenic bacterium of Panax notoginseng round spot disease (acerus spp.). Contamination by other miscellaneous bacteria can obtain comparatively pure conidia suspension; For sporulation amount, the present invention can produce 2 * 10 in each petri dish on average 4 conidia; the method is low in cost, and general laboratories can meet the requirements of sporulation conditions of the present invention.