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The role of vitamin B12 in the metabolism of Euglena gracilis var. bacillaris.

The Biochemical Journal, 01 Aug 1965, 96(2):552-556
https://doi.org/10.1042/bj0960552 PMID: 5837793 PMCID: PMC1207074

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Abstract 

1. The concentrations of RNA, DNA and protein are decreased in cells of Euglena gracilis var. bacillaris grown on suboptimum concentrations of vitamin B(12). 2. The addition of vitamin B(12) to deficient cells stimulates the incorporation of [(14)C]formate into the above cell components as well as into thymine of DNA and serine and methionine of protein. 3. In a cell-free system from vitamin B(12)-deficient cells, the incorporation of labelled formate into thymidylate is decreased to a greater extent with uridine than with deoxyuridine as the substrate. 4. The addition of unlabelled glutamate dilutes the radioactivity incorporated into thymine from labelled formate. 5. These results are interpreted to mean that, in DNA synthesis, vitamin B(12) has a greater role in the reduction of ribotides to deoxyribotides than in the reduction of formate to thymine methyl and that the vitamin B(12)-dependent conversion of glutamate into beta-methylaspartate also contributes to thymine synthesis.

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Biochem J. 1965 Aug; 96(2): 552–556.
PMCID: PMC1207074
PMID: 5837793

The role of vitamin B12 in the metabolism of Euglena gracilis var. bacillaris

S. Venkataraman, M. S. Netrawali, and A. Sreenivasan
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Abstract

1. The concentrations of RNA, DNA and protein are decreased in cells of Euglena gracilis var. bacillaris grown on suboptimum concentrations of vitamin B12. 2. The addition of vitamin B12 to deficient cells stimulates the incorporation of [14C]formate into the above cell components as well as into thymine of DNA and serine and methionine of protein. 3. In a cell-free system from vitamin B12-deficient cells, the incorporation of labelled formate into thymidylate is decreased to a greater extent with uridine than with deoxyuridine as the substrate. 4. The addition of unlabelled glutamate dilutes the radioactivity incorporated into thymine from labelled formate. 5. These results are interpreted to mean that, in DNA synthesis, vitamin B12 has a greater role in the reduction of ribotides to deoxyribotides than in the reduction of formate to thymine methyl and that the vitamin B12-dependent conversion of glutamate into β-methylaspartate also contributes to thymine synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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