Fig 1.
FISH analysis on S. scintillans.
(A-D) RCC257 and (E-L) RCC257-jp showing no endobacterial signals. (A), (E) and (J) Brightfield; (B) and (F) DAPI; (C) and (G) EUB388 probe under 473 and 488 nm; (D) merged image of (A-C); (H) CF319 probe under 647 nm; (I) merged image of (E-H); (K) merged image of unstained DAPI, CF319 and (L) EUB388 images. Scale bars = 5 μm.
Fig 2.
Prasinovirus multi-gene tree and a virus-like particle (VLP).
(A) and (B) Detection of VLP in negatively stained RCC24. (B) Close up of the VLP in (A). The diameter of the VLP is 192 nm. Scale bars = 100 nm. (C) A multi-gene prasinovirus phylogeny reconstructed from 22 core genes (5,213 sites) using IQ-TREE2 LG+F+G4 model. The right panel shows presence-absence of select core genes. Single-copy genes are DNApol (DNA polymerase B), DNAhel-SNF2 (SNF2 helicase), mRNAcap (mRNA capping enzyme), ATPase, and RNR-sm (RNR small subunit). The tree is rooted with Chlorovirus (PBCVs and ATCV) for visualization. Only nodes <100% ultrafast bootstrap supports are labelled. OlV = Ostreococcus lucimarinus virus; OtV = Ostreococcus tauri virus; OmV = Ostreococcus mediterraneus virus; MpV = Micromonas pusilla virus; BpV = Bathycoccus prasinos virus; BIIV = Bathycoccus sp. virus clade BII. PBCV = Paramecium bursaria chlorella virus; ATCV = Acanthocystis turfaceae chlorella virus.
Fig 3.
Genome overview and comparison of select vMAGs to corresponding reference genomes.
Circularized representation of (A) RCC257 BpV-vMAG compared to BpV2 genome; (B) OlV1 genome compared to RCC257 OlV1-vMAG; (C) MpV_Pl1 genome compared to MpVPl1_vMAG, in an ordered set of coding sequences, represented by blocks shaded by similarity. The sizes of vMAGs labelled in the centre. (A) Mapping coverage is based on RCC257 BpV-vMAG mapped to viral-subset-scaffolds and regions with the coverage more than one standard deviation [62.1] from the mean coverage [50.8] are shown in blue spikes. The outermost ring represents predicted ORFs of the vMAG with manually annotated protein from Prodigal-gv and Viralrecall. (B) Mapping coverage is based on OlV1 genome mapped to viral-subset-scaffolds and regions with the coverage more than one standard deviation [8.4] from the mean coverage [3.1] shown in blue spikes. Only ORFs from the reference OlV1 genome is shown and the partial RCC257 OlV1-vMAG CDS are shown in the outermost ring. (C) Mapping coverage is based on MpV_Pl1 genome mapped to viral-subset-scaffolds and regions with the coverage more than one standard deviation [7.8] from the mean coverage [0.6] shown in blue spikes. Only ORFs from the reference OlV1 genome is shown and the partial RCC257 OlV1-vMAG CDS are shown in the outermost ring. See S2 Table for annotation in a tabular format.