Fig 1.
The size distuibution of the assembled unigenes from C.cunea male and female antennal transcriptomes.
Fig 2.
The best hits of the BLASTn results.
All C. cunea antennal unigenes were used in BLASTn to search the GenBank entries. The best hits with an E-value = 1.0E-5 for each query were grouped according to species.
Fig 3.
Gene Ontology (GO) classifications of the male and female C. cunea antennal unigenes according to their involvement in biological processes, cellular component and molecular function.
Fig 4.
C. cunea OBPs transcript levels in different body parts as evaluated by RT-PCR.
GADPH was used as a control for the integrity of each cDNA template. MA: male antennae; FA: female antennae; MH: male heads; FH: female heads; MT: male thoraxes; FT: female thoraxes; MB: male abdomen; FB: male abdomen. Black graphic indicats robust amplification defined as a clearly detectable amplimer in the agarose gel, gray graphic indicates faint amplication, “-” indicates no amplification. Antennae specific or enriched genes are labeled with a capital letter “A”.
Fig 5.
C. cunea ORs transcript levels in different body parts as evaluated by RT-PCR.
GADPH was used as a control for the integrity of each cDNA template. MA: male antennae; FA: female antennae; MH: male heads; FH: female heads; MT: male thoraxes; FT: female thoraxes; MB: male abdomen; FB: male abdomen. Black graphic indicats robust amplification defined as a clearly detectable amplimer in the agarose gel, gray graphic indicates faint amplication, “-” indicates no amplification. Antennae specific or enriched genes are labeled with a capital letter “A”.
Fig 6.
C. cunea GRs, CSPs, IRs and SNMP1 transcript levels in different body parts as evaluated by RT-PCR.
GADPH was used as a control for the integrity of each cDNA template. MA: male antennae; FA: female antennae; MH: male heads; FH: female heads; MT: male thoraxes; FT: female thoraxes; MB: male abdomen; FB: male abdomen. Black graphic indicats robust amplification defined as a clearly detectable amplimer in the agarose gel, gray graphic indicates faint amplication, “-” indicates no amplification. Antennae specific or enriched genes are labeled with a capital letter “A”.
Fig 7.
C.cunea OBPs transcript levels in different tissues as measured by RT-qPCR.
MA: male antennae; FA: female antennae; MH: male head; FH: female head; MT: male thorax; FT: female thorax; MB: male abdomen; FB: female abdomen. The glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used to normalize transcript levels in each sample. The standard error is represented by the error bar, and the different letters (a, b, c) above each bar denote significant differences (p<0.05).
Fig 8.
C.cunea ORs transcript levels in different tissues as measured by RT-qPCR.
MA: male antennae; FA: female antennae; MH: male head; FH: female head; MT: male thorax; FT: female thorax; MB: male abdomen; FB: female abdomen. The glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used to normalize transcript levels in each sample. The standard error is represented by the error bar, and the different letters (a, b, c) above each bar denote significant differences (p<0.05).
Fig 9.
C.cunea GRs transcript levels in different tissues as measured by RT-qPCR.
MA: male antennae; FA: female antennae; MH: male head; FH: female head; MT: male thorax; FT: female thorax; MB: male abdomen; FB: female abdomen. The glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used to normalize transcript levels in each sample. The standard error is represented by the error bar, and the different letters (a, b, c) above each bar denote significant differences (p<0.05).
Fig 10.
C.cunea CSPs transcript levels in different tissues as measured by RT-qPCR.
MA: male antennae; FA: female antennae; MH: male head; FH: female head; MT: male thorax; FT: female thorax; MB: male abdomen; FB: female abdomen. The glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used to normalize transcript levels in each sample. The standard error is represented by the error bar, and the different letters (a, b, c) above each bar denote significant differences (p<0.05).
Fig 11.
C.cunea IRs and SNMP transcript levels in different tissues as measured by RT-qPCR.
MA: male antennae; FA: female antennae; MH: male head; FH: female head; MT: male thorax; FT: female thorax; MB: male abdomen; FB: female abdomen. The glyceraldehyde-3-phosphate dehydrogenase (GADPH) was used to normalize transcript levels in each sample. The standard error is represented by the error bar, and the different letters (a, b, c) above each bar denote significant differences (p<0.05).