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Metabolomic fingerprinting of Brazilian marine sponges: a case study of Plakinidae species from Fernando de Noronha Archipelago

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Abstract

Marine sponges from the Plakinidae family are well known for hosting cytotoxic secondary metabolites and the Brazilian Atlantic coast and its oceanic islands have been considered as a hotspot for the discovery of new Plakinidae species. Herein, we report the chemical profile among cytotoxic extracts obtained from four species of Plakinidae, collected in Fernando de Noronha Archipelago (PE, Northeastern Brazil). Crude organic extracts of Plakinastrella microspiculifera, Plakortis angulospiculatus, Plakortis insularis, and Plakortis petrupaulensis showed strong antiproliferative effects against two different cancer cell lines (HCT-116: 86.7–100%; MCF-7: 74.9–89.5%) at 50 μg/mL, by the MTT assay. However, at a lower concentration (5 μg/mL), high variability in inhibition of cell growth was observed (HCT-116: 17.3–68.7%; MCF-7: 0.00–55.5%), even within two samples of Plakortis insularis which were collected in the west and east sides of the Archipelago. To discriminate the chemical profile, the samples were investigated by UHPLC-HRMS under positive ionization mode. The produced data was uploaded to the Global Natural Products Social Molecular Networking and organized based on spectral similarities for purposes of comparison and annotation. Compounds such as dipeptides, nucleosides and derivatives, polyketides, and thiazine alkaloids were annotated and metabolomic differences were perceived among the species. To the best of our knowledge, this is the first assessment for cytotoxic activity and chemical profiling for Plakinastrella microspiculifera, Plakortis insularis and Plakortis petrupaulensis, revealing other biotechnologically relevant members of the Plakinidae family.

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Acknowledgements

We are forever grateful to Prof. Dr. Rosângela de Almeida Epifanio (in memoriam) for her dedication to the research in Brazilian marine natural products. She collected, kept, and cared for the biological material we assessed in this study, and her provision is the essential feature of all the work carried out until this day by our research group.

Funding

The authors thank Coordination for the Improvement of Higher Education Personnel (CAPES, Finance Code 001), Council for Technological and Scientific Development (CNPq) (grant number 406064/2018-0) CNPq, FAPERJ and São Paulo Research Foundation (FAPESP) [grant number 2014/50244-6; 2015/17177-6] for financial support.

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Authors and Affiliations

  1. Laboratório de Produtos Naturais (LAPROMAR), Instituto de Química, Universidade Federal Fluminense, Niterói, RJ, 24020-005, Brazil

    Thayssa da Silva F. Fagundes, Larissa Ramos G. da Silva, Mateus de Freitas Brito, Thatyana Rocha A. Vasconcelos & Alessandra Leda Valverde

  2. SEPARARE -Núcleo de Pesquisa em Cromatografia, Departamento de Química, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil

    Larissa Ramos G. da Silva & Quezia Bezerra Cass

  3. Laboratório de Bioprospecção de Organismos Marinhos, Instituto do Mar, Universidade Federal de São Paulo, Santos, SP, 11070-100, Brazil

    Letícia S. S. Schmitz, Dhiego B. Rigato & Paula Christine Jimenez

  4. Grupo de Produtos Naturais de Organismos Aquáticos (GPNOA), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro, Macaé, RJ, 27965-045, Brazil

    Angélica Ribeiro Soares

  5. Laboratório de Farmacologia Marinha, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, 05508-000, Brazil

    Letícia V. Costa-Lotufo

  6. Laboratório de Porifera, Museu Nacional, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 20940-040, Brazil

    Guilherme Muricy

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  1. Thayssa da Silva F. Fagundes
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Correspondence to Alessandra Leda Valverde.

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Fagundes, T.d.S.F., da Silva, L.R.G., Brito, M.d.F. et al. Metabolomic fingerprinting of Brazilian marine sponges: a case study of Plakinidae species from Fernando de Noronha Archipelago. Anal Bioanal Chem 413, 4301–4310 (2021). https://doi.org/10.1007/s00216-021-03385-6

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