Abstract
BioDeNOx is a combined physicochemical and biological process for the removal of nitrogen oxides (NOx) from flue gas. In the present study, two anaerobic bioreactors performing BioDeNOx were run consecutively (RUN-1 and RUN-2) at a dilution rate of 0.01 h−1 with Fe(II)EDTA.NO2− and Fe(III)EDTA− as electron acceptors and ethanol as electron donor. The measured protein concentration of the reactor biomass of both runs was 120 mg/l. Different molecular methods were used to determine the identity and abundance of the bacterial populations in both bioreactors. Bacillus azotoformans strain KT-1 was recognized as a key player in Fe(II)EDTA.NO2− reduction. PCR-denaturing gradient gel electrophoresis analysis of the reactor biomass showed a greater diversity in RUN-2 than in RUN-1. Enrichments of Fe(II)EDTA.NO2− and Fe(III)EDTA− reducers and activity assays were conducted using the biomass from RUN-2 as an inoculum. The results on substrate turnover, overall microbial diversity, and enrichments and finally activity assays confirmed that ethanol was used as electron donor for Fe(II)EDTA.NO2− reduction. In addition, the Fe(III)EDTA− reduction rate of the microbial community proved to be feasible enough to run the bioreactors, ruling out the chemical reduction of Fe(III)EDTA− with sulfide as was proposed by other researchers.
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Amann RI, Binder BJ, Olson RJ, Chisholm SW, Devereux R, Stahl DA (1990) Combination of 16S ribosomal-RNA-targeted oligonucleotide probes with flow-cytometry for analyzing mixed microbial-populations. Appl Environ Microbiol 56:1919–1925
Boon M (1996) Theoretical and experimental methods in determining rate-limiting subprocesses. In: Theoretical and experimental methods in the modeling of bio-oxidation kinetics of sulphide minerals. Thesis from the Delft University of Technology, Delft, The Netherlands
Buisman CJN, Dijkman H, Verbraak PL, den Hartog AJ (1999) Process for purifying flue gas containing nitrogen oxides. U.S. Patent 5,891,408
Cao X, Liu X, Dong X (2003) Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. Int J Syst Evol Microbiol 53:971–975
Daims H, Bruhl A, Amann R, Schleifer K-H, Wagner M (1999) The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol 22:434–444
Fachgruppe Wasserchemie GDC (1991) Deutsche Einheitsverfahren zur Wasser-Abwasser-und Schlamm-Untersuchung, vol. Bd. II, E1. VCH, Weinheim, Germany
Greene AC, Patel CBK, Sheehy AJ (1997) Deferribacter thermophilus gen. nov., sp. nov., a novel thermophilic manganese-and iron-reducing bacterium isolated from a petroleum reservoir. Int J Syst Bacteriol 47:505–509
Gries-Romijn-van Eck (1966) Physiological and chemical test for drinking water. NEN 1056, IY-2 Nederlandse Normalisatie Instituut Rijswijk, The Netherlands
Janssen PH, Liesack W, Schink B (2002) Geovibrio thiophilus sp. nov., a novel sulfur-reducing bacterium belonging to the phylum Deferribacteres. Int J Syst Evol Microbiol 52:1341–1347
Kumaraswamy R, van Dongen U, Kuenen JG, van Loosdrecht MCM, Muyzer G (2005) Characterization of microbial communities removing nitrogen oxides from flue gas: the BioDeNOx process. Appl Environ Microbiol 71:6345–6352
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar Buchner A, Lai T, Steppi S, Jobb G, Forster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, Konig A, Liss T, Lussmann R, May M, Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, Bode A, Schleifer K-H (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371
Maas van der P, van de Sandt T, Klapwijk B, Lens P (2003) Biological reduction of nitric oxide in aqueous Fe(II)EDTA solutions. Biotechnol Prog 19:1323–1326
Maas van der P, Harmsen L, Weelink S, Klapwijk B, Lens P (2004) Denitrification in aqueous FeETDA solutions. J Chem Technol Biotechnol 79:835–841
Maas van der P, Peng S, Klapwijk B, Lens P (2005) Enzymatic versus nonenzymatic conversions during the reduction of EDTA chelated Fe(III) in BioDeNOx reactors. Environ Sci Technol 39(8):2616–2623
Manz W, Amann RI, Ludwig W, Wagner M, Schleifer K-H (1992) Phylogenetic and oligonucleotide probes for major subclasses of proteobacteria: problems and solutions. Syst Appl Microbiol 15:593–600
Meier H, Amann RI, Ludwig W, Schleifer K-H (1999) Specific oligonucleotide probes for in situ detection of a major group of Gram-positive bacteria with low DNA G+C content. Syst Appl Microbiol 22:186–196
Neef A, Zaglauer A, Meier H, Amann R, Lemmer H, Schleifer K-H (1996) Population analysis in a denitrifying sand filter: conventional and in situ identification of Paracoccus spp. in methanol-fed biofilms. Appl Environ Microbiol 62:4329–4339
Otte S, Grobben NG, Robertson LA, Jetten MSM, Kuenen JG (1996) Nitrous oxide production by Alcaligenes faecalis under transient and dynamic aerobic and anaerobic conditions. Appl Environ Microbiol 62:2421–2426
Pernthaler J, Glockner FO, Schönhuber W, Amann R (2001) Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes. In: Paul JH (ed) Methods in microbiology, marine microbiology, vol 30. Academic, New York, pp 207–226
Schäfer H, Muyzer G (2001) Denaturing gradient gel electrophoresis in marine microbial ecology. In: Paul JH (ed) Methods in microbiology, marine microbiology, vol. 30. Academic, New York, pp 425–468
Visser A, Beeksman I, van der Zee F, Stams AJM, Lettinga G (1993) Anaerobic degradation of volatile fatty acid at different sulfate concentrations. Appl Microbiol Biotechnol 40:549–556
Ye Q, Roh Y, Carroll SL, Blair B, Zhou J, Zhang CL, Fields MW (2004) Alkaline anaerobic respiration: isolation and characterization of a novel alkaliphilic and metal-reducing bacterium. Appl Environ Microbiol 70:5595–5602
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This work was supported by the Dutch Science Foundation for Applied Research (STW).
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Kumaraswamy, R., Kuenen, J.G., Kleerebezem, R. et al. Structure of microbial communities performing the simultaneous reduction of Fe(II)EDTA.NO2−and Fe(III)EDTA− . Appl Microbiol Biotechnol 73, 922–931 (2006). https://doi.org/10.1007/s00253-006-0542-z
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DOI: https://doi.org/10.1007/s00253-006-0542-z