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Wickerhamomyces kurtzmanii sp. nov. An Ascomycetous Yeast Isolated From Crater Lake Water, Da Hinggan Ling Mountain, China

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Abstract

One novel ascomycetous yeast strain TF5-16-2 was isolated from water samples of Tuofengling crater lake located in Da Hinggan Ling Mountain, in the Inner Mongolia province of China. Morphological, physiological characteristics, as well as phylogenetic analyses of D1/D2 domains of the large subunit rRNA (LSU), ITS region, small subunit rRNA (SSU), and elongation factor-1α (EF-1α) were performed and finally confirmed the phylogenetic placement of strain TF5-16-2 in the genus Wickerhamomyces. Sequences analysis revealed that strain TF5-16-2 differed from its most closely related phylogenetic neighbors ‘Candida’ silvicultrix CBS 6269T and Wickerhamomyces anomalus CBS 5759T by 8.0% (including 2.3% gaps), 8.5% (including 2.4% gaps) divergences in D1/D2 domains of LSU, and 11% (including 4.3% gaps) and 13% (including 4.4% gaps) divergences in ITS region, respectively. As the considerable sequence divergence and distinguishable physiological characteristics, strain TF5-16-2 was proposed as a new species of the genus Wickerhamomyces, with the name Wickerhamomyces kurtzmanii sp. nov. (holotype = CGMCC 2.5597, Mycobank number is MB829959).

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Abbreviations

LSU:

Large subunit rRNA

ITS:

Internal transcribed spacer;

SSU:

Small subunit rRNA

EF-1α:

Elongation factor-1α

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Acknowledgement

The authors gratefully acknowledge financial support from the National Natural Science Funds of China (31400106) and the National Science and Technology Foundation Project (2014FY210400). We thank Dr. Chun-Li Li for his kind help in the use of scanning electron microscopes.

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Authors and Affiliations

  1. China General Microbiological Culture Collection Center and State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Bi-Si Jia, Ai-Hua Li & Yu-Guang Zhou

  2. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Pei-Jie Han & Qi-Ming Wang

  3. School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, 300314, China

    Yu Zhou

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  1. Yu Zhou
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Zhou, Y., Jia, BS., Han, PJ. et al. Wickerhamomyces kurtzmanii sp. nov. An Ascomycetous Yeast Isolated From Crater Lake Water, Da Hinggan Ling Mountain, China. Curr Microbiol 76, 1537–1544 (2019). https://doi.org/10.1007/s00284-019-01773-x

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  • DOI: https://doi.org/10.1007/s00284-019-01773-x

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