Abstract
Camellia reticulata is a well-known woody ornamental species endemic to Southwest China. It represents a polyploid complex with diploids, allotetraploids, and allohexaploids. The parentage of the allotetraploids and allohexaploids has been reported by genomic in situ hybridization, but the maternal parents still remain unknown. In this study, sequences of the chloroplast rpl16 intron of 105 cultivars of C. reticulata and 7 congeneric species were used to infer the maternal origin of the allopolyploids. The results showed that (1) the allotetraploids were derived from C. pitardii as the maternal parental species and the diploid C. reticulata as the paternal parental species; (2) the allohexaploid C. reticulata was derived from the allotetraploid C. reticulata as the maternal parent and C. saluenensis as the paternal parent; and (3) the C. reticulata cultivars were derived from hexaploid C. reticulata as the maternal parents. These results indicated that C. pitardii, the allotetraploid and allohexaploid C. reticulata may serve as good potential maternal parents for the cross breeding of Camellia.
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Acknowledgements
We are grateful to Baojun Feng, Shi Feng, Zhonglang Wang, and Wei Yi for assistance with sample collections.
Funding
This work is supported by the National Natural Science Foundation of China (31101565, 31360488, 31660228), the China Scholarship Council, and Laboratory of Analytical Biology of the Smithsonian Institution.
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Xu, X., Zheng, W., Harris, A. et al. Assessing the maternal origin in the polyploid complex of Camellia reticulata based on the chloroplast rpl16 intron sequences: implications for camellia cross breeding. Mol Breeding 38, 123 (2018). https://doi.org/10.1007/s11032-018-0883-1
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DOI: https://doi.org/10.1007/s11032-018-0883-1