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Sexual introgression of the late blight resistance gene Rpi-blb3 from a Mexican wild diploid species Solanum pinnatisectum Dunal into potato varieties

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Abstract

Solanum pinnatisectum Dunal (2n = 2x = 24) is a tuber-bearing Mexican wild species that has an extremely high level of resistance to late blight (Phytophthora infestans). However, this species is distantly related and isolated from cultivated potato species by a strict sexual reproduction barrier. Previously, we successfully obtained triploid interspecific hybrids between chromosome-doubled S. pinnatisectum and a South American diploid clone carrying the S locus inhibitor gene (Sli). In this study, chromosome doubling of triploid hybrids was done to produce hexaploids used as the recurrent and donor parents in the successive backcrossing scheme. In a BC2 population consisting of 198 genotypes, P. infestans was inoculated. Ninety-six genotypes were resistant, and 102 were susceptible. The presence of the Rpi-blb3–specific marker band perfectly matched the resistance phenotype, verifying that the Rpi-blb3 gene controlled resistance. BC1 and BC2 progenies were relatively easy to reciprocally cross with cultivars. Therefore, the Rpi-blb3 gene derived from S. pinnatisectum could be used in practical breeding to confer a high level of late blight resistance to potato varieties with the aid of the gene-specific marker.

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Acknowledgments

We thank Dr. Seishi Akino of Hokkaido University for providing the isolate of P. infestans, Dr. Shinji Kikuchi of Chiba University for the chromosome observations by the DAPI staining method, Dr. Meki Muktar for preparing the P. infestans inoculum, and Eri Kakuta for the technical assistance.

Funding

Calbee, Inc.; Hokkaido Potato Growers Association; Kewpie Corp.; KENKO Mayonnaise Co., Ltd.; Japan Snack Cereal Foods Association; and Stabilization Fund Association of Hokkaido Potato Production financially supported this study.

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  1. Potato Germplasm Enhancement Laboratory, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan

    Rena Sanetomo & Kazuyoshi Hosaka

  2. Nagasaki Agricultural and Forestry Technical Development Center, 3118 Kaizu, Isahaya, Nagasaki, 854-0063, Japan

    Ippei Habe

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  1. Rena Sanetomo
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Sanetomo, R., Habe, I. & Hosaka, K. Sexual introgression of the late blight resistance gene Rpi-blb3 from a Mexican wild diploid species Solanum pinnatisectum Dunal into potato varieties. Mol Breeding 39, 13 (2019). https://doi.org/10.1007/s11032-018-0924-9

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