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Effect of Funneliformis mosseae and Thiobacillus thioparus on sulfur utilization in soybean sterilized soil under continuous cropping

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Abstract

Purpose

This study was to investigate the regulation of sulfur status in continuous-cropping soybean soil by Funneliformis mosseae and Thiobacillus thioparus and promote the absorption of sulfur in soybean. To explore the interactions among F. mosseae, T. thioparus and soybean plants, this study laid a theoretical foundation for the application of F. mosseae and T. thioparus as biological agents in agricultural production.

Methods

Pot culture, a two-compartment system and shake-flask culture were used in the experiment. Using F. mosseae and T. thioparus as experimental inoculants, the effects of F. mosseae on sulfur oxide of T. thioparus and their interaction on the growth of soybean plants were studied from the aspects of soil sulfur content, sulfur functional genes and the growth of soybean and sulfur-oxidizing bacteria.

Results

The F. mosseae and T. thioparus inoculation significantly increased the abundance of sulfur-oxidizing bacteria and available sulfur content in the soil, stimulated the expression of sulfate transporter genes in soybean roots, and promoted the absorption of sulfur nutrients in soybean. F. mosseae stimulated the expression of the T. thioparus sulfur-oxidation gene and enhanced the sulfur-oxidation capacity, while T. thioparus enhanced F. mosseae colonization of soybean roots. Thus, F. mosseae and T. thioparus promoted soil sulfur cycling and sulfate transport in soybean roots, which proved that F. mosseae could effectively improve the sulfur-oxidation capacity of T. thioparus.

Conclusion

Double inoculation with F. mosseae and T. thioparus significantly promoted soybean plant growth and increased soil sulfur utilization.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 31972502).

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Author notes

  1. Ronglin Liu and Donghao Chang contributed equally to this work.

Authors and Affiliations

  1. Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Molecular Biology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, 150080, China

    Ronglin Liu, Donghao Chang, Zixin Sun, Yunshu Wu, Xueqi Zhang, Chengcheng Lu, Yizhi Mao, Ji Chen & Baiyan Cai

  2. Hebei Key Laboratory of Agroecological Safety, Hebei University of Environmental Engineering, Qinhuangdao, 066102, China

    Baiyan Cai

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  1. Ronglin Liu
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Correspondence to Baiyan Cai.

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Liu, R., Chang, D., Sun, Z. et al. Effect of Funneliformis mosseae and Thiobacillus thioparus on sulfur utilization in soybean sterilized soil under continuous cropping. Plant Soil 490, 357–370 (2023). https://doi.org/10.1007/s11104-023-06081-9

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