Skip to main content
SpringerLink
Log in

Three new arbuscular mycorrhizal Diversispora species in Glomeromycota

  • Original Article
  • Published:
Mycological Progress Aims and scope Submit manuscript

Abstract

Morphological observations of spores and mycorrhizal structures of three arbuscular mycorrhizal fungi (Glomeromycota) prompted, and subsequent phylogenetic analyses of SSU–ITS–LSU nrDNA sequences confirmed, that they are undescribed species of the genus Diversispora. Morphologically, the first species, here named D. varaderana, is most distinguished by its relatively small (≤90 μm diam when globose) and yellow-coloured spores with a simple spore wall consisting of two layers, of which layer 1, forming the spore surface, is short-lived and usually completely sloughed in most spores. The distinctive features of the second species, D. peridiata, are the occasional formation of spores in clusters and peridium-like hyphae covering the clusters and single spores, and especially the permanent and relatively thick spore wall layer 1, which is the only coloured component of the two-layered spore wall of the yellow-coloured and relatively small spores (≤100 μm diam). The third species, D. slowinskiensis, is most characterized by its spore wall layer 1 that is the only coloured component of the three-layered spore wall and frequently is covered with blister-like swellings. All the three species were grown in single-species cultures established from spores extracted from trap cultures inoculated with rhizosphere soils of plants growing in maritime sand dunes: D. varaderana from those located near Varadero on the Hicacos Peninsula, Cuba, and the two others from those of the Słowiński National Park, northern Poland.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Figs. 2–8
Figs. 10–17
Figs. 18–25

Similar content being viewed by others

References

  • Balazs TK, Błaszkowski J, Chwat G, Góralska A, Gaspar BK, Lukacs AF, Kovács GM (2015) Spore-based study of arbuscular mycorrhizal fungi of semiarid sandy areas in Hungary, with Diversispora jakucsiae sp. nov. Mycol Prog 14:1021. 10.1007/s11557-014-1021-z

  • Błaszkowski J (1994) Polish Glomales 11 Glomus pustulatum. Mycorrhiza 4:201–207. doi:10.1007/BF00206781

  • Błaszkowski J (1995) Glomus corymbiforme, a new species in Glomales from Poland. Mycologia 87:732–737. doi:10.2307/3760819

    Article  Google Scholar 

  • Błaszkowski J (1997) Glomus gibbosum, a new species from Poland. Mycologia 89:339–345. doi:10.2307/3761092

    Article  Google Scholar 

  • Błaszkowski J (2012) Glomeromycota. W. Szafer Institute of Botany, Polish Academy of Sciences, Kraków

  • Błaszkowski J, Tadych M, Madej T (2001) Glomus arenarium, a new species in Glomales (Zygomycetes). Acta Soc Bot Pol 70:97–101. doi:10.5586/asbp.2001.013

    Article  Google Scholar 

  • Błaszkowski J, Adamska I, Czerniawska B (2003) Glomus trimurales, an arbuscular mycorrhizal fungus (Glomerales) new for Poland and Europe. Mycotaxon 87:425–436

    Google Scholar 

  • Błaszkowski J, Adamska I, Czerniawska B (2004) Glomus insculptum, a new arbuscular mycorrhizal species from Poland. Mycotaxon 89:225–234

    Google Scholar 

  • Błaszkowski J, Kovács GM, Gáspár BK, Balázs TK, Buscot F, Ryszka P (2012) The arbuscular mycorrhizal Paraglomus majewskii sp. nov. represents a new distinct basal lineage in Paraglomeraceae (Glomeromycota). Mycologia 104:148–156. doi:10.3852/10-430

    Article  PubMed  Google Scholar 

  • Błaszkowski J, Chwat G, Kovács GM, Gáspár BK, Ryszka P, Orłowska E, Pagano MC, Araújo FS, Wubet T, Buscot F (2013) Septoglomus fuscum and S. furcatum, two new species of arbuscular mycorrhizal fungi (Glomeromycota). Mycologia 105:670–680. doi:10.3852/12-127

    Article  PubMed  Google Scholar 

  • Błaszkowski J, Chwat G, Góralska A, Ryszka P, Orfanoudakis M (2014) Septoglomus jasnowskae and Septoglomus turnauae, two new species of arbuscular mycorrhizal fungi (Glomeromycota). Mycol Prog 13(4):999–1009. doi:10.1007/s11557-014-0985-z)

    Article  Google Scholar 

  • Błaszkowski J, Chwat G, Góralska A (2015a) Acaulospora ignota and Claroideoglomus hanlinii, two new species of arbuscular mycorrhizal fungi (Glomeromycota) from Brazil and Cuba. Mycol Prog 14:18. doi:10.1007/s11557-015-1042-2

    Article  Google Scholar 

  • Błaszkowski J, Chwat G, Góralska A, Ryszka P, Kovács GM (2015b) Two new genera, Dominikia and Kamienskia, and D. disticha sp. nov. in Glomeromycota. Nova Hedwigia 100:225–238. doi:10.1127/nova_hedwigia/2014/0216

    Google Scholar 

  • Błaszkowski J, Chwat G, Symanczik S, Góralska A (2015c) Dominikia duoreactiva and D. difficilevidera, two new species in the Glomeromycota. Botany. doi:10.1139/cjb-2015-0016

    Google Scholar 

  • Börstler B, Thiéry O, Sýkorová Z, Berner A, Redecker D (2010) Diversity of mitochondrial large subunit rDNA haplotypes of Glomus intraradices in two agricultural field experiments and two semi-natural grasslands. Mol Ecol 19:1497–1511. doi:10.1111/j.1365-294X.2010.04590.x

    Article  PubMed  Google Scholar 

  • Corradi N, Kuhn G, Sanders IR (2004) Monophyly of β-tubulin and H + −ATPase gene variants in Glomus intraradices: consequences for molecular evolutionary studies of AM fungal genes. Fungal Genet Biol 41:262–273. doi:10.1016/j.fgb.2003.11.001

    Article  CAS  PubMed  Google Scholar 

  • Estrada B, Palenzuela J, Barea J-M, Ruiz-Lozano JM, Alves G, da Silva GA, Oehl F (2011) Diversispora clara (Glomeromycetes) - a new species from saline dunes in the Natural Park Cabo de Gata (Spain). Mycotaxon 118:73–81. doi:10.5248/118.73

    Article  Google Scholar 

  • Gamper HA, Walker C, Schüßler A (2009) Diversispora celata sp. nov: molecular ecology and phylotaxonomy of an inconspicuous arbuscular mycorrhizal fungus. New Phytol 182:495–506. doi:10.1111/j.1469-8137.2008.02750.x

    Article  CAS  PubMed  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755. doi:10.1093/Bioinformatics/17.8.754

    Article  CAS  PubMed  Google Scholar 

  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780. doi:10.1093/molbev/mst010

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kornerup A, Wanscher JH (1983) Methuen handbook of colour, 3rd edn. Eyre Methuen, London

    Google Scholar 

  • Koske RE, Friese C, Walker C, Dalpé Y (1986) Glomus pustulatum: a new species in the Endogonaceae. Mycotaxon 26:143–149

    Google Scholar 

  • Krüger M, Krüger C, Walker C, Stockinger H, Schüßler A (2012) Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level. New Phytol 193:970–984. doi:10.1111/j.1469-8137.2011.03962.x

    Article  PubMed  Google Scholar 

  • Msiska Z, Morton J (2009) Phylogenetic analysis of the Glomeromycota by partial β-tubulin gene sequences. Mycorrhiza 19:247–254. doi:10.1007/s00572-008-0216-z

    Article  CAS  PubMed  Google Scholar 

  • Nagy LG, Kocsubé S, Csanádi Z, Kovács GM, Petkovits T, Vágvölgyi C, Papp T (2012) Re-mind the gap! Insertion – deletion data reveal neglected phylogenetic potential of the nuclear ribosomal internal transcribed spacer (ITS) of fungi. PLoS ONE 7, e49794. doi:10.1371/journal.pone. 0049794

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Nylander JAA, Wilgenbusch JC, Warren DL, Swofford DL (2007) AWTY (Are We There Yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics 24:581–583. doi:10.1093/bioinformatics/btm388

    Article  PubMed  Google Scholar 

  • Oehl F, da Silva GA, Goto BT, Sieverding E (2011a) Glomeromycota: three new genera and glomoid species reorganized. Mycotaxon 116:75–120. doi:10.5248/116.75

    Article  Google Scholar 

  • Oehl F, da Silva GA, Sánchez-Castro I, Goto BT, Maia LC, Vieira HEE, Barea J-M, Sieverding E, Palenzuela J (2011b) Revision of Glomeromycetes with entrophosporoid and glomoid spore formation with three new genera. Mycotaxon 117:297–316. doi:10.5248/117.297

    Article  Google Scholar 

  • Omar MB, Bollan L, Heather WA (1979) A permanent mounting medium for fungi. B Brit Mycol Soc 13:31–32. doi:10.1016/S0007-1528(79)80038-3

    Article  Google Scholar 

  • Palenzuela J, Ferrol N, Boller T, Azcón-Aquila C, Oehl F (2008) Otospora bareai, a new fungal species in the Glomeromycetes from a dolomitic shrub-land in the natural park of Sierra de Baza (Granada, Spain). Mycologia 100:296–305. doi:10.3852/mycologia.100.2.296

    Article  CAS  PubMed  Google Scholar 

  • Piotrowska H (1991) The development of the vegetation in the active deflation hollows of the Łeba Bar (N Poland). Frag Flor Geobot 35:173–215

    Google Scholar 

  • Redecker D, Raab P, Oehl F, Camacho FJ, Courtecuisse R (2007) A novel clade of sporocarp-forming species of glomeromycotan fungi in the Diversisporales lineage. Mycol Prog 6:35–44. doi:10.1007/s11557-007-0524-2

    Article  Google Scholar 

  • Redecker D, Schüβler A, Stockinger H, Stürmer SL, Morton JB, Walker C (2013) An evidence-based consensus for the clasification of arbuscular mycorrhizal fungi (Glomeromycota). Mycorrhiza 23:515–531. doi:10.1007/s00572-013-0486-y

    Article  PubMed  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. doi:10.1093/bioinformatics/btg 180

    Article  CAS  PubMed  Google Scholar 

  • Schüßler A, Walker C (2010) The Glomeromycota: a species list with new families and new genera. Gloucester, England

  • Schüßler A, Schwarzott D, Walker C (2001) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Myc Res 105:1413–1421. doi:10.1017/S0953756201005196

    Article  Google Scholar 

  • Schüßler A, Krüger M, Walker C (2011) Revealing natural relationships among arbuscular mycorrhizal fungi: culture line BEG47 represents Diversispora epigaea, not Glomus versiforme. PLoS ONE 6(8), e23333. doi:10.1371/journal.pone.0023333

    Article  PubMed Central  PubMed  Google Scholar 

  • Silvestro D, Michalak I (2012) raxmlGUI: A graphical front-end for RAxML. Org Divers Evol 12:335–337. doi:10.1007/s13127-011-0056-0

    Article  Google Scholar 

  • Simmons MP, Ochoterena H, Carr TG (2001) Incorporation, relative homoplasy, and effect of gap characters in sequence-based phylogenetic analyses. Syst Biol 50:454–462. doi:10.1080/106351501300318049

    Article  CAS  PubMed  Google Scholar 

  • Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic, San Diego

    Google Scholar 

  • Sokolski S, Dalpé Y, Séguin S, Khasa D, Lévesque A, Pitché Y (2010) Conspecificity of DAOM 197198, the model arbuscular mycorrhizal fungus, with Glomus irregulare: molecular evidence with three protein-encoding genes. Botany 88:829–838. doi:10.1139/B10-050

    Article  CAS  Google Scholar 

  • Stamatakis A (2014) RAxML version 8: a tool phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Stockinger H, Peyret-Guzzon MP, Koegel S, Bouffaud M-L, Redecker D (2014) The largest subunit of RNA polymerase II as a New marker gene to study assemblages of arbuscular mycorrhizal fungi in the field. PLoS ONE 9(10), e107783. doi:10.1371/journal.pone.0107783

    Article  PubMed Central  PubMed  Google Scholar 

  • Stürmer SL, Morton JB (1997) Developmental patterns defining morphological characters in spores of four species in Glomus. Mycologia 89:72–81. doi:10.2307/3761174

    Article  Google Scholar 

  • Sudová R, Sýkorová Z, Rydlová J, Čtvrtlíková M, Oehl F (2015) Rhizoglomus melanum, a new arbuscular mycorrhizal fungal species associated with submerged plants in freshwater lake Avsjøen in Norway. Mycol Prog 14:9. doi:10.1007/s11557-015-1031-5

    Article  Google Scholar 

  • Sýkorová Z, Börster B, Zvolenská S, Fehrer J, Gryndler M, Vosátka M, Redecker D (2012) Long-term tracing of Rhizophagus irregularis isolate BEG140 inoculated on Phalaris arundinaceae in a coal mine spoil bank, using mitochondrial large subunit rDNA markers. Mycorrhiza 1:69–80. doi:10.1007/s00572-011-0375-1

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Thiéry O, Moora M, Vasar M, Zobel M, Öpik M (2012) Inter- and intrasporal nuclear ribosomal gene sequence variation within one isolate of arbuscular mycorrhizal fungus, Diversispora sp. Symbiosis 58:135–147. doi:10.1007/s13199-012-0212-0

    Article  Google Scholar 

  • van der Heijden MGA, Martin FM, Selosse M-A, Sanders IR (2015) Mycorrhizal ecology and evolution: the past, the present, and the future. New Phytol 205:1406–1423. doi:10.1111/nph.13288

    Article  PubMed  Google Scholar 

  • Walker C (1983) Taxonomic concepts in the Endogonaceae: spore wall characteristics in species descriptions. Mycotaxon 18:443–455

    Google Scholar 

  • Walker C, Schüßler A (2004) Nomenclatural clarifications and new taxa in the Glomeromycota. Mycol Res 108:979–982. doi:10.1017/S0953756204231173

    Article  Google Scholar 

  • Walker C, Vestberg M, Demircik F, Stockinger H, Saito M, Sawaki H, Nishmura I, Schüβler A (2007) Molecular phylogeny and new taxa in the Archaeosporales (Glomeromycota): Ambispora fennica gen. sp. nov., Ambisporaceae fam. nov., and emendation of Archaeospora and Archaeosporaceae. Mycol Res 111:137–153. doi:10.1016/j.mycres.2006.11.008

    Article  PubMed  Google Scholar 

  • Young ND, Healy J (2003) GapCoder automates the use of indel characters in phylogenetic analysis. BMC Bioinformatics 4:6. doi:10.1186/1471-2105-4-6

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported in part by Polish National Centre of Science, grants no. 2012/05/B/NZ8/00498 and 2012/07/N/NZ8/02363. Gábor M. Kovács is supported by a János Bolyai Research Scholarship of the Hungarian Academy of Sciences. We thank the assistant manager of the Institute of Ecology and Systematic, Havana, Cuba and the manager of the Słowiński National Park, Poland for permitting us to collect rhizosphere soil samples.

Author information

Authors and Affiliations

  1. Department of Ecology and Protection of Environment, West Pomeranian University of Technology in Szczecin, Słowackiego 17, PL–71434, Szczecin, Poland

    Janusz Błaszkowski, Gerard Chwat & Anna Góralska

  2. Instituto de Ecología e Sistemática, Carretera Varona 11835 e/ Oriente y Lindero, La Habana 19 Calabazar, CP 11900, Boyeros, La Habana, Cuba

    Eduardo Furrazola

  3. Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University Pázmány Péter sétány 1/C, H-1117, Budapest, Hungary

    Alena F. Lukács & Gábor M. Kovács

Authors
  1. Janusz Błaszkowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eduardo Furrazola
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gerard Chwat
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anna Góralska
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Alena F. Lukács
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gábor M. Kovács
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Janusz Błaszkowski.

Additional information

Section Editor: Marco Thines

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Błaszkowski, J., Furrazola, E., Chwat, G. et al. Three new arbuscular mycorrhizal Diversispora species in Glomeromycota. Mycol Progress 14, 105 (2015). https://doi.org/10.1007/s11557-015-1122-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11557-015-1122-3

Keywords

Search

Navigation