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Identification of volatile components and growth promoting effects of endophytes of Tinospora capillipes Gagnep.

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Abstract

Plant diseases seriously affect the agricultural economy and eco-environment. Recently, the agricultural application of endophytes has received considerable attention due to its environmentally friendly characteristics. In this study, endophytes of Tinospora capillipes Gagnep. were isolated, and antagonistic endophytes were screened to explore their biological characteristics and promoting effects, which can lay the foundation for exploring green biocontrol strains. Based on the identification results, two endophytes with antagonistic effect of plant pathogens were identified as Epicoccum nigrum and Arthrinium phaeospermum respectively. Both strains possessed various extracellular enzyme activities, phosphorus hydrolysis and low Indole-3-acetic acid (IAA) production capacity. A. phaeospermum showed potassium-solubilizing ability while E. nigrum lacked this ability. Both spore suspension and fermentation supernatant of E. nigrum could promote soybean seeds rooting at low concentration. The spore suspension of A. phaeospermum had obvious promoting effect on seeds rooting at high concentration while its fermentation supernatant did not. The main composition of volatile components of A. phaeospermum was phenylethyl alcohol (2-PE). The co-culture of A. phaeospermum with plants could promote the growth of maize seedlings’ taproots, indicating that A. phaeospermum was able to be exploited as sustainable and environment-friendly biocontrol strain in agriculture.

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Funding

This study was funded by the Zhejiang Provincial Natural Science of Foundation of China [Grant number LQ20B070005] and Zhejiang Provincial Fund for Outstanding Young Talents of Traditional Chinese Medicine [Grant number 2020ZQ013].

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Authors and Affiliations

  1. School of Life Sciences, Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang, People’s Republic of China

    Qianxi Li & Senmiao Chen

  2. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, 310053, Hangzhou, Zhejiang, People’s Republic of China

    Siyu Wang, Fangmei Zhou, Zhishan Ding & Xiaoqing Ye

  3. School of Basic Medicine, Southeast university, 210009, Nanjing, Jiangsu, People’s Republic of China

    Yini Shang

  4. Puer Kunhong Biotechnology Company, Group C of Chamagu Town A, Simao District, 665000, Puer, Yunnan, People’s Republic of China

    Jie Yu

Authors
  1. Qianxi Li
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  2. Siyu Wang
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  4. Yini Shang
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  5. Fangmei Zhou
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Contributions

All authors contributed to the study conception and design. The experiments were designed by Zhishan Ding, Xiaoqing Ye, and Qianxi Li. The experiments were performed by Qianxi Li, Siyu Wang, Senmiao Chen and Yini Shang. The data analysis was performed by Qianxi Li, Siyu Wang and Xiaoqing Ye. The technical support was provided by Fangmei Zhou and Jie Yu. The first draft of the manuscript was written by Qianxi Li and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xiaoqing Ye.

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Li, Q., Wang, S., Chen, S. et al. Identification of volatile components and growth promoting effects of endophytes of Tinospora capillipes Gagnep.. J Plant Pathol 105, 57–68 (2023). https://doi.org/10.1007/s42161-022-01282-0

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  • DOI: https://doi.org/10.1007/s42161-022-01282-0

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