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Morphological and molecular characterization of Nothopassalora personata from Argentina

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Abstract

Late leaf spot (LLS) is the most important foliar disease of peanuts in Argentina and in other peanut production areas, causing defoliation and yield losses. In this study, six isolates obtained from symptomatic leaflets collected in Córdoba, Argentina, were characterized based on the morphology of the colony and conidia, as well as molecularly using a combination of the 28S nrRNA gene (LSU), the internal transcribed spacer regions and the intervening 5.8S nrRNA gene (ITS) of the rDNA operon, and the second largest subunit of RNA polymerase II (RPB2). Pathogenicity was confirmed using the detached leaf method, inoculating a spore suspension. Mycelial growth was evaluated on four culture media: peanut oatmeal agar (POA), potato dextrose agar (PDA), peanut leaflet extract agar (PLA) and peanut seed extract agar (PSA), with PSA and PDA showing the greatest radial growth. This is the first study of N. personata obtained in the peanut-producing region of Argentina.

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Acknowledgements

This work was financially supported by FONCyT (BID-PICT-2018-04057) and INTA (2019-PE-E4-I086-001).

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Authors and Affiliations

  1. Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, IPAVE-CIAP-INTA, X5020, Córdoba, Argentina

    Joaquín Humberto Monguillot, Nelson Bernardi Lima, Juan Andrés Paredes & Cinthia Conforto

  2. Unidad de Fitopatología y Modelización Agrícola, Consejo Nacional de Investigaciones Científicas Y Técnicas, UFyMA-CONICET, X5020, Córdoba, Argentina

    Joaquín Humberto Monguillot, Nelson Bernardi Lima, Juan Andrés Paredes, Alejandro Mario Rago & Cinthia Conforto

  3. Facultad de Ciencias Agrarias, UNCA, Catamarca, Argentina

    Nelson Bernardi Lima

  4. Facultad de Agronomía y Veterinaria, IMICO, UNRC, Córdoba, Argentina

    Damián Francisco Giordano & Claudio Oddino

  5. Centro de Investigaciones Agropecuarias - INTA, Córdoba, Argentina

    Alejandro Mario Rago

  6. Facultad de Agronomía, Universidad de Buenos Aires, Catedra de Fitopatología, Buenos Aires, Argentina

    Marcelo Carmona

Authors
  1. Joaquín Humberto Monguillot
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  2. Nelson Bernardi Lima
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  4. Damián Francisco Giordano
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  8. Cinthia Conforto
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Correspondence to Cinthia Conforto.

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Monguillot, J.H., Lima, N.B., Paredes, J.A. et al. Morphological and molecular characterization of Nothopassalora personata from Argentina. J Plant Pathol 105, 557–565 (2023). https://doi.org/10.1007/s42161-022-01292-y

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