Pre-Pulse Inhibition Startle Protocol

Prepulse Inhibition of Startle (PPI) is a behavioral phenomenon in which a short tone presented just prior to an acoustic startle stimulus (a sound presented at a fairly high amplitude) will serve to dampen the subsequent startle response.

PPI has been observed in mice, rats, Rhesus Macaques, and humans. Rodent testing of PPI is used in the testing of therapeutic compounds, and has been used as a screening technique for transgenic/knock-out mice.

• Included in the Startle Reflex Package (MED-ASR-PRO1)
• Specify frequency (Hz) and amplitude (dB)
  • NOTE: Protocol code cannot be edited, for custom experiment design use the Startle Reflex Pro software (SOF-828)

 

Protocol Summary

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Pre-pulse

• Eight (8) trials @ 20 KHz / 70 dB
• Eight (8) trials @ 12 KHz / 70 dB
• Eight (8) trials @ 4 KHz / 70 dB

Noise Burst Only

• Acclimation period of one (1) minute
• Twenty-four (24) PPI trials consisting of:
  • Eight (8) trials @ 20 KHz / 70 dB
  • Eight (8) trials @ 12 KHz / 70 dB
  • Eight (8) trials @ 4 KHz / 70 dB
• PPI trials have sixteen (16) randomly interspersed noise burst only trials @ 100 dB

Noise Burst

• Fifteen (15) trials randomly presented
• Each trial separated by a variable ITI range of 3 – 8 seconds
• Intensity = 70, 80, or 90 dB

SKU:   SOF-826