Fig_7.tif (834 kB)
Protein 169 increases 80S monosomes and decreases polysomes.
figure
posted on 2015-09-03, 02:47 authored by Pavla Strnadova, Hongwei Ren, Robert Valentine, Michela Mazzon, Trevor R. Sweeney, Ian Brierley, Geoffrey L. SmithHEK 293 Trex 169 cells were uninduced (A) or induced (B) with DOXY (1 μg/ml) for 14 h. Cytoplasmic cells lysates were prepared and resolved on 10–50% sucrose density gradients (Methods). The gradient was fractionated and fractions monitored by absorbance (A254 nm). Indicated fractions were collected and proteins were extracted, resolved by SDS-PAGE and analyzed by immunoblotting using the indicated antibodies. The positions of molecular size markers in kDa are indicated on the left and right. WCL = whole cell lysate. Data shown are from one representative experiment (n = 4).
History
Usage metrics
Categories
Keywords
80 S ribosomesTranslation initiationReserve protein 169VACV strainhost protein synthesisVaccinia Virus Strategycell cultureAdaptive immunityprotein 169 causescytoplasmic polypeptidevirus factoriesvirus challengeprototypic orthopoxvirusSuppress Innateintradermal murine modelslung weightIntranasal infectionEctopic Expressionvirus suppressionVirus Virulence Vaccinia virusProtein expressionv Δ169revertant control viruses
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC