Total Phosphorus Methods of Analysis, Wastewater Treatment AND EFFECTS ON Nature Denice Johnson, Chemist NEORSD.

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1 Total Phosphorus Methods of Analysis, Wastewater Treatment AND EFFECTS ON Nature Denice Johnson, Chemist NEORSD

2 What is Phosphorus? Phosphorus (P) is a nutrient that is vital to human, animal, and plant growth. It’s one of the most common substances found in nature. It’s found in our water, our food, and our bodies.

3 What is Phosphorus? It’s a highly reactive, nonmetallic element existing in three allotropic forms, white (or sometimes yellow), red, and black. In its pure form, it is toxic.

4 Where does it come from? Phosphorus occurs naturally at low levels in water, plants, and animals. Phosphorus occurs unnaturally in fertilizers (used in agriculture), cleaners (used in industry) and wastewater (from household sewage). Phosphorus is found in water, solids (detritus), and in the bodies of biological organisms.

5 So why is it important? We need it to survive in low levels!
However, high levels of Phosphorus in waterways causes eutrophication, an excess richness of nutrients, which leads to algal blooms and/or dense plant growth. This process decreases sunlight and oxygen levels (hypoxia) thus affecting fish and other aquatic life.

6 So why is it important?

7 Algae – Lake Erie

8 Algal Bloom - Pond

9 Forms of Phosphorus Phosphorus (P) occurs mostly as Phosphates (PO4).
These are classified as Orthophosphates (reactive phosphates), Condensed Phosphates (pyro, meta, and polyphosphates) and Organic Phosphates.

10 Orthophosphates Orthophosphates, also known as Reactive Phosphates, are a main constituent in fertilizers used for agriculture and residential purposes. Orthophosphates can be carried into streams and lakes through run-off.

11 Condensed Phosphates Condensed (inorganic) phosphates are phosphorus compounds that contain salts and/or metals such as sodium, potassium, and calcium in various structures and chains. Condensed phosphates are used in industry and as food additives.

12 Did you know? Phosphoric acid was (and still is in dark varieties) added to pop to enhance flavor and fizz. However, phosphoric acid can leach calcium from bones and teeth. High levels of phosphorus in the body can cause premature aging and cancer.

13 Organic Phosphates Organic Phosphates are formed primarily by biological processes (ex: ATP – Adenosine Triphosphate). ATP is a chemical compound that breaks down to release energy in the body. Organic phosphates enter sewage via human waste and food residues. Organic phosphates can be formed from orthophosphates in biological treatment processes or by receiving water biota.

14 Phosphorus Analysis Collection, Preservation and Holding Times
Ortho vs Total Phosphorus Dissolved Phosphorus Different Methods of Analysis Digestion of Total Phosphorus Quality Control (QC) samples Interferences

15 Collection and Preservation
Collection: Plastic or glass Hold Time: Total Phosphorus: 28 days Orthophosphate: 48 hours Preservation: Total Phosphorus: pH<2 H2SO Orthophosphate: No Preservation  Storage: Refrigerate, <4oC

16 Orthophosphate vs. Total Phosphorus
Orthophosphate (reactive) is analyzed directly on an unpreserved sample within 48 hours of sampling. Total Phosphorus (all forms) is analyzed on an acid preserved sample within 28 days of sampling following an acid digestion.

17 Dissolved Phosphorus Dissolved Reactive Phosphorus samples are field filtered, within 15 minutes of sampling, through a 0.45-mm filter and analyzed within 48 hours. Low Level Dissolved Reactive Phosphorus samples are treated the same as above, except the filter size is 0.2-mm. Dissolved Total Phosphorus samples are filtered through a 0.45-mm filter prior to preservation. The sample is then digested and analyzed within 28 days of sampling.

18 Phosphorus Methods Colorimetric EPA Method – Manual Spectrophotometer Semi-Automated Colorimetric EPA Method – Automated Spectrophotometer (Flow injection Analysis –FIA) Standard Methods 4500-P Ion Chromatography (IC) EPA Method 300.0 Discrete Analyzers

19 Discrete Analyzer A instrument that employs robotics and syringes to aspirate, dispense, and mix appropriate amounts of sample and reagents into reaction wells. The sample/reagent mixture then incubates in the reaction well for a pre-programmed time. A single aliquot of the mixture is then transferred to a cuvette for spectrophotometric analysis. Absorbance is plotted against ppm in a linear regression curve to calculate concentration. Can analyze the various forms of phosphorus.

20 Discrete Analyzer Advantages: Reduces manual labor Reduces reagents
Reduces sample volume Reduces waste Disadvantages: Instrument cost

21 Ion Chromatography Anions are separated based on their affinities toward the stationary phase in the column. A suppressor cartridge chemically suppresses the background conductance of the eluent (dilute KOH solution) and converts the anions into species of higher conductance.

22 Ion Chromatography Using pressure and eluent, the sample is “pushed” through a stationary phase column. The anions are separated by size and then measured using a conductivity detector. The electronic signal is converted to peak area. Concentration is determined using a linear regression curve (plotting area vs. ppm).

23 Ion Chromatography

24 Ion Chromatography Analyzes Reactive (Ortho) Phosphate only
Linear Range mg/L for Phosphate Calibrate monthly Minimum of 4 Standards covering the linear range, from which a linear regression curve is created. >0.995 Correlation Coefficient Method Detection Limits (MDL) performed following new MDL rules with quarterly checks

25 Linear Regression Curve ION CHROMATOGRAPHY

26 IC Analysis QC Initial Calibration Verification (ICV) and Initial Calibration Blank (ICB) analyzed daily Continuing Calibration Verification (CCV) and Continuing Calibration Blank (CCB) analyzed initially before samples, after every 10 samples and again at the end of the sequence Reporting Limit Check (RLC) analyzed with each batch containing Drinking Water samples

27 IC Batch QC Laboratory Control Blank (LCB) and Laboratory Control Sample (LCS) prepared fresh daily per batch of 20 samples Duplicate/Matrix Spike or MS/MSD per 10 samples. Percent Recovery and Relative Percent Difference (RPD) are calculated

28 IC Sample Preparation All samples and batch QC (LCB, LCS, Duplicates, MS/MSD) are filtered through a 0.20-mm syringe filter to remove all particulate. MS/MSD’s are spiked as to be within the analysis range.

29 IC Interferences All solids must be filtered out. Any particulate in the system will cause problems and plug up the system. High concentrations of some ions will cause false readings of other ions. Peak shifting: as the column gets older, peaks may shift causing misidentification.

30 IC Advantages/Disadvantages
Analyzes up to 7 Anions in less than 15 minutes Can be very stable Can run overnight (load and go!) No hazardous waste Small sample volume needed May have to do multiple dilutions per sample Each injection takes up to 30 minutes to run

31 Phosphorus 365.1/365.2 Phosphorus is analyzed using colorimetric (spectrophotometric) analysis. This can be performed manually or automated. Orthophosphate (Reactive Phosphorus) is analyzed directly. Total Phosphorus (as P) is analyzed after going through an acid digestion to convert all forms of phosphorus to orthophosphate.

32 Method Summary Ammonium molybdate and antimony potassium tartrate react in a acid medium with orthophosphate to form a phosphomolybdic acid, which is then reduced to an intense molybdenum blue color by ascorbic acid. The resultant color and read spectrophotometrically at 880-nm. This can be performed on a manual spectrophotometer or on an automated FIA spectrophotometer.

33 Orthophosphate (Reactive)
Orthophosphate (reactive) is analyzed directly on an unpreserved sample within 48 hours of sampling. No preparation needed for Manual Spectrophotometer For Automated Spectrophotometer, samples must be filtered though Whatman 41 filter paper to remove solids Dissolved Reactive Phosphorus and Low Level Dissolved Reactive Phosphorus filtered through 0.45-mm and 0.2-mm syringe filter, respectively, IN THE FIELD

34 Orthophosphate (Reactive)
Calibrate Daily Linear Range mg/L Minimum of 5 Standards covering the linear range, from which a linear regression is created. >0.995 Correlation Coefficient Method Detection Limits (MDL) performed following new MDL rules with quarterly checks.

35 Linear Regression Curve
Orthophosphate Final results are obtained from the linear regression curve created with the known standard concentrations. Linear Regression Curve

36 Analysis QC Initial Calibration Verification (ICV) and Initial Calibration Blank (ICB) analyzed daily Continuing Calibration Verification (CCV) and Continuing Calibration Blank (CCB) analyzed initially before samples, after every 10 samples and again at the end of the sequence

37 Batch QC Laboratory Control Blank (LCB) and Laboratory Control Sample (LCS) prepared fresh daily per batch of 20 samples Duplicate/Matrix Spike or MS/MSD per 10 samples. Percent Recovery and Relative Percent Difference (RPD) are calculated

38 Total Phosphorus Method Summary: Total Phosphorus analysis has two procedural steps: ONE - convert all phosphorus forms to orthophosphate using acid hydrolysis. TWO - analyze orthophosphate colorimetrically, either manually or automated.

39 Total Phosphorus Digestion
In the acid hydrolysis step, sulfuric acid and ammonium persulfate (peroxydisulfate) are added to a known volume of sample. It is then digested on a hotplate to near dryness. The sample is cooled and diluted back to the original volume. Sample is then filtered to remove any solids for Auto- Analysis. Alternatively, an autoclave may be used for digestion (30 min at 121oC, psi) or hotblock. Both are a closed digestion, therefore no evaporation nor dilution required (30 min at 150oC).

40 Total Phosphorus Analysis
The colorimetric step, is the same as the orthophosphate in that, Ammonium molybdate and antimony potassium tartrate react in a acid medium with orthophosphate to form a phosphomolybdic acid, which is then reduced to an intense molybdenum blue color by ascorbic acid. The resultant color and read spectrophotometrically at 880-nm The colorimetric step may be performed on a manual spectrophotometer or on an automated analyzer.

41 Total Phosphorus Analysis
Calibrate Daily Linear Range mg/L (ours mg/L) Minimum of 5 Standards covering the linear range from which a linear regression is created. >0.995 Correlation Coefficient Method Detection Limits (MDL) performed following new MDL rules with quarterly checks

42 Linear Regression Curve
Total Phosphorus Final results are obtained from the linear regression curve created with the known standard concentrations. Linear Regression Curve

43 Analysis QC Initial Calibration Verification (ICV) and Initial Calibration Blank (ICB) analyzed daily Continuing Calibration Verification (CCV) and Continuing Calibration Blank (CCB) analyzed initially before samples, after every 10 samples and again at the end of the sequence

44 Batch QC Laboratory Control Blank (LCB) and Laboratory Control Sample (LCS) digested fresh daily per batch of 20 samples Duplicate/Matrix Spike or MS/MSD per every 10 samples. Percent Recovery and Relative Percent Difference (RPD) are calculated

45 QC Failure & Corrective Actions
Calibration Criteria, such as residuals or correlation coefficient out of range, results in recalibrating the instrument. Analysis QC criteria not met, results in re-analyzing the initial or continuing QC. Repeated failure results in re- calibrating the instrument. Batch QC results out of acceptable range may result in re- digestion and/or re-analysis of QC and samples.

46 Phosphorus Interferences
As with any colorimetric analysis, turbidity (solids) can be a positive interference but can be removed with filtration. Filtration must occur prior to analysis, not prior to digestion (unless the sample is dissolved) on total phosphorus samples. Sample color may also interfere. Background correction can be performed in this case.

47 Phosphorus Interferences
High levels of arsenic can cause a positive interference if the As concentrations are higher than the phosphorus concentration. High levels of iron may cause the precipitation of, and subsequently the loss of phosphorus.

48 Troubleshooting Contamination!! Contamination!! Contamination!!
All glassware must be washed with hot water and phosphate free soap, rinsed with dilute hydrochloric acid and rinsed with copious amounts of de-ionized water. All instrument components and reagents must be phosphorus free.

49 Troubleshooting FIA Auto-Spectrophotometers: Plugs and leaks are the most common problems. Routine Maintenance helps prevent problems: -Changing pump and tygon tubing -Rinsing and/or cleaning manifold tubing -Keeping area clean and dust free -Keeping reagents covered when in use

50 Treatment of Phosphorus
Some of the Phosphorus entering the treatment plant is removed by primary (settling) and secondary treatment. However, phosphorus remains in the solids. Additional phosphorus is removed by adding Ferric Chloride, Ferrous Sulfate, Alum, or Lime during the waste treatment process.

51 Treatment of Phosphorus
Chemical removal Ferric Chloride Fe3++ (HnPO4)3-n ↔ FePO4 + nH+ Aluminum Sulfate (Alum) Al3++ (HnPO4)3-n ↔ AlPO4 + nH+

52 Phosphorus Denice Johnson Chemist Northeast Ohio Regional Sewer District 4747 East 49th Street Cuyahoga Heights, Ohio Office: X2501 Mobile:

53 The Nitrogen Cycle and Analysis
Paul Skerl Senior Wastewater Analyst NE Ohio Regional Sewer District

54 Outline Nitrogen Importance Forms Cycle and Fixation Process
Contributing Factors and Effects Analysis Total Kjeldahl Nitrogen (TKN) Nitrate, Nitrite, Nitrate+Nitrite Ammonia Nitrogen

55 Why is Nitrogen Important
Nitrogen is one of the main components of Amino Acids, Proteins, and Nucleic Acids (DNA) Nitrogen is a limiting nutrient in plant growth All living organisms need nitrogen to survive 78% of Earth’s atmosphere is nitrogen

56 Oxidation States of Nitrogen
Nitrogen has the ability to exist in seven different oxidation states: Ammonia NH3 Molecular Nitrogen N2 Nitrous Oxide N2O Nitric Oxide NO Dinitrogen Trioxide N2O2 Nitrogen Oxide NO2 Dinitrigen Pentoxide N2O5 Three forms, when combined with water, result in inorganic ionized species that are of historical environmental concern in surface and drinking waters.

57 Forms of Nitrogen Occurring in Waters
Ammonia → Ammonium NH3 + H2O → NH4+ + OH- Note: For our purposes Ammonia Nitrogen includes both forms as NH3 and NH4+ Dinitrogen Trioxide → Nitrite N2O3 + H2O → 2H+ + 2NO2- Dinitrogen Pentoxide → Nitrate N2O5 + H2O → H+ + 2NO3-

58 Forms of Nitrogen Occurring in Waters
Organic Nitrogen Organic Nitrogen or Total Organic Nitrogen (TON), is an organically bound Nitrogen in a tri-negative oxidation state, …O3- Total Kjeldahl Nitrogen (TKN) Organic Nitrogen + Ammonia Nitrogen Total Nitrogen Total Nitrogen = TKN + Nitrate + Nitrite

59 Comprehensive Nitrogen Cycle Diagram
Ammonia NH3 Nitrite ion Dead Organic Matter NO2- NO2- Animal Protien Nitrate ion NO3- Plant Matter

60 Nitrogen Fixation Processes
Biological fixation: some symbiotic bacteria (most often associated with leguminous plants) and some free-living bacteria are able to fix nitrogen and assimilate it as organic nitrogen. Industrial N-fixation : in the Haber-Bosch process, N2 is converted together with hydrogen gas (H2) into ammonia (NH3) which is used to make fertilizer and explosives. Combustion of fossil fuels : automobile engines and thermal power plants, which release various nitrogen oxides (NOx). Other processes : Additionally, the formation of NO from N2 and O2 due to photons and especially lightning, are important for atmospheric chemistry, but not for terrestrial or aquatic nitrogen turnover.

61 Contributing Factors / Sources
Ammonia Nitrogen Agriculture Runoff from fertilizers Natural organic decomposition Industrial Point sources

62 Contributing Factors / Sources
Ammonia Nitrogen Domestic wastewater Aquaculture Manure Application

63 Contributing Factors / Sources
Nitrates and Nitrites Pasture and rangeland runoff Agricultural and irrigation runoff

64 Contributing Factors / Sources
Nitrates and Nitrites Industrial Effluent Landscape runoff: golf courses, athletic fields Rodenticides Food preservatives

65 Environmental Factors/Effects
Ammonia nitrogen includes both NH3 and NH4+. Its presence in water has varying toxic affects on fish and aquatic organisms. The more toxic unionized NH3 increases as pH increases. Low pH reaction is driven to right NH3 + H2O NH4+ + OH- High pH reaction is driven to left

66 Environmental Factors/Effects
Furthermore, as temperature increases total ammonia activity increases = higher toxicity. Depending upon the aquatic conditions, ammonia nitrogen toxicity can: Cause gill damage Affect fish growth Cause fish kills

67 Environmental Factors/Effects
Excess ammonia, nitrates and nitrites (plant nutrients) can contribute to eutrophication, a dense overgrowth of blue-green algae and other plant life, in lakes and waterways.

68 Environmental Factors/Effects
This dense overgrowth, or algae bloom, disturbs the normal function of the ecosystem, resulting in low dissolved oxygen, needed by fish and other aquatic life to survive. Another cause of fish kill. The entire ecosystem becomes unbalanced, whereas native species can be overtaken by invasive species, or the whole system may collapse X X X

69 Analytical Analysis TKN: Total Kjeldahl Nitrogen
Nitrate, Nitrite, Nitrate+Nitrite Ammonia Nitrogen: Undistilled and Distilled

70 TKN Methods listed in 40CFR
EPA Method 351.1 Total Kjeldahl Nitrogen Automated Phenate colorimetric. EPA Method 351.3 Standard Methods 4500-NH3. Total Kjeldahl Nitrogen Digestion and Distillation followed by one of following Titration Nesslerization Electrode. EPA Method 351.4 Total Kjeldahl Nitrogen Manual or block digester potentiometric.

71 TKN Methods listed in 40CFR
EPA Method [NEORSD] Standard Methods 4500-Norg C Total Kjeldahl Nitrogen Semi-Automated block digester colorimetric. Collection and Holding Times Plastic or glass pH < 2 (H2SO4) 28 days < 60C

72 TKN The sample is digested using a strong acid solution with potassium sulfate and a cupric sulfate catalyst. The digestate is then analyzed colorimetrically on an automated spectrophotometer at a wavelength of 660nm. Digestion Procedure utilizes copper sulfate as a catalyst to convert biological nitrogen compounds (amino acids, proteins and peptides) into Ammonia.

73 TKN QC digested per batch Aqueous Solid LCB LCS OLCS
Duplicate and Matrix Spike Solid

74 TKN Interferences Large amount of Organic Matter
Digestate diluted to Volume The digestate is then analyzed colorimetrically on an automated spectrophotometer at a wavelength of 660nm. Interferences Large amount of Organic Matter Inorganic Salts and solids Sample Turbidity Color

75 TKN Rinse tubing and manifold Pump Reagents through Manifold Reagents
Turn on Computer, Instrument and peripheral devices to prepare for analysis. Rinse tubing and manifold Pump Reagents through Manifold Reagents Buffer NaOH 0.8M Bleach Carrier Salicylate Nitroprusside

76 TKN Analyze Curve 6 standards 0.5 – 20 ppm And Initial QC ICV 5 ppm
Set up Curve and Samples Autosampler and enter information into software Analyze Curve 6 standards 0.5 – 20 ppm And Initial QC ICV 5 ppm ICB < 0.5 ppm CCV 10 ppm CCB < 0.5 ppm

77 TKN Determine if Curve and QC meet criteria set forth in SOP, as well as peaks having proper shape and area similar to historically acceptable runs. Analyze Samples, LCB, LCS, OLC and continuing QC if data is acceptable as per SOP, report results.

78 Nitrate, Nitrite, Nitrate-Nitrite Methods listed in 40CFR
EPA Method 352.1 Nitrate (as N) Colorimetric (Brucine sulfate), or Nitrate-Nitrite N minus Nitrite N EPA Method 353.3 Standard Methods 4500-NO3-E. Nitrate-Nitrite (as N) Cadmium reduction , Manual. EPA Method 353.1 Standard Methods 4500-NO3-H Nitrate-Nitrite (as N) Cadmium reduction , Automated hydrazine.

79 Nitrate, Nitrite, Nitrate-Nitrite Methods listed in 40CFR
EPA Method [NEORSD] Standard Methods 4500-NO3-F Nitrate-Nitrite (as N) Cadmium reduction , Automated . Collection and Holding Times Plastic or glass pH < 2 (H2SO4) 28 days < 60C

80 Nitrate, Nitrite, Nitrate-Nitrite Methods listed in 40CFR
Note: If reporting a Nitrate value as well as a Nitrite value, or either one independently, Nitrite must be run as well. NO2NO3 – NO2 = NO3 Standard Methods 4500-NO2- B [NEORSD] Nitrite (as N) Colorimetric Method Collection and Holding Times Plastic or glass Unpreserved 48 hours < 60C

81 Nitrate, Nitrite, Nitrate+Nitrite
Turn on Computer, Instrument and peripheral devices to prepare for analysis. Rinse tubing and manifold Pump Reagents through Manifold Reagents Carrier Sulfanilamide Color Reagent Ammonium Chloride Buffer Open Cadmium Column

82 Nitrate, Nitrite, Nitrate+Nitrite
Open Cadmium Column Nitrate is quantitatively reduced to nitrite by passage through a copperized cadmium column. The color is then developed and the sample (NO3 + NO2) is read at 520nm and is measured colormetricaly.

83 Nitrate, Nitrite, Nitrate+Nitrite
Interferences Residual Chlorine High Fe, Cu, or other metals High Oil and Grease Color and Turbidity Prior to Analysis for both methods, samples, LCB, LCS, Duplicates and Matrix Spikes require filtration, accomplished gravimetrically through a rinsed Whatman 41 Filter paper.

84 Nitrate, Nitrite, Nitrate+Nitrite
Set up Curve and Samples Autosampler and enter information into software Analyze Curve 6 standards 0.02 – 2.0 ppm And Initial QC ICV: ppm NO2+NO3, 0.75ppm NO2 ICB: < 0.02 ppm DLC: 15 ppm NO2+NO3, 7.5 ppm NO2 CCV: 1 ppm NO2+NO3, 0.5 ppm NO2 CCB: < 0.02 ppm Additional QC for Subtraction NO2: 1 ppm LRC: 2 ppm NO3 NO3 CCV: 1 ppm

85 Nitrate, Nitrite, Nitrate+Nitrite
Determine if Curve and QC meet criteria set forth in SOP, as well as peaks having proper shape and area similar to historically acceptable runs. Analyze Samples, LCB, LCS, and continuing QC if data is acceptable as per SOP, report results.

86 Ammonia Methods listed in 40CFR
EPA Method 350.2 Standard Methods 4500-NH3 C. Ammonia (as N) Manual Distillation followed by Nesslerization, or Titration. EPA Method 350.3 Standard Methods NH3 D or E Ammonia (as N) Manual Distillation followed by Electrode.

87 Ammonia Methods listed in 40CFR
EPA Method [NEORSD] Standard Methods 4500-NH3 G Ammonia (as N) Manual Distillation followed by Automated Phenate. Collection and Holding Times Plastic or glass pH < 2 (H2SO4) 28 days < 60C Note: For all Methods, in instances of drinking waters, clean surface or ground water, the distillation step is not required. Furthermore, if a valid distillation study (comparing filtered to distilled samples has been demonstrated for routine WWTP and river samples), the distillation step can be eliminated.

88 Ammonia Nitrogen Samples requiring distillation are distilled with the curve and QC, and are run separately from non-distilled samples

89 Ammonia Nitrogen QC Distilled per Batch Curve standards 0.10-20ppm
ICV 5 ppm ICB CCV 10 ppm CCB Aqueous LCB LCS Duplicate and Matrix Spike Solid

90 Ammonium Nitrogen Distillate diluted to Volume The distillate is then analyzed colorimetrically on an automated spectrophotometer at a wavelength of 630nm. This procedure is based on the Berthelot reaction where ammonia reacts with alkaline phenol and sodium hypochlorite to form indophenol blue Interferences Cyanates Residual Chlorine Ca and Mg Ions Turbitity and Color Contamination

91 Ammonia Nitrogen Pump Reagents through Manifold
Turn on Computer, Instrument and peripheral devices to prepare for analysis. Rinse tubing and manifold Pump Reagents through Manifold Reagents Carrier Bleach Buffer Phenolate Sodium Nitroprosside

92 Ammonia Nitrogen Analyze Curve 6 standards 0.02 – 2.0 ppm
Set up Curve and Samples Autosampler and enter information into software Analyze Curve 6 standards 0.02 – 2.0 ppm And Initial QC ICV: 1 ppm ICB: < 0.02 ppm DLC: 10 ppm CCV: 0.5 ppm CCB: < 0.02 ppm

93 Ammonia Nitrogen Determine if Curve and QC meet criteria set forth in SOP, as well as peaks having proper shape and area similar to historically acceptable runs. Analyze Samples, LCB, LCS and continuing QC, if data is acceptable as per SOP, report results.

94 Ammonia Nitrogen Non-distilled samples are analyzed simultaneously for Ammonia with Nitrate+Nitrite. Therefore, as with Nitrate + Nitrite, prior analysis, filter through rinsed Whatman 41 paper. Same as distilled samples, this procedure is based on the Berthelot reaction where ammonia reacts with alkaline phenol and sodium hypochlorite to form indophenol blue. Interferences Cyanates Residual Chlorine Ca and Mg Ions Turbitity and Color Contamination

95 Ammonia Nitrogen Pump Reagents through Manifold
Turn on Computer, Instrument and peripheral devices to prepare for analysis. Rinse tubing and manifold Pump Reagents through Manifold Reagents Carrier* Bleach Buffer* Phenolate Sodium Nitroprosside

96 Ammonia Nitrogen Analyze Curve 6 standards 0.1 – 20 ppm And Initial QC
Set up Curve and Samples Autosampler and enter information into software Analyze Curve 6 standards 0.1 – 20 ppm And Initial QC ICV: 5 ppm ICB: < 0.1 ppm CCV: 10 ppm CCB: < 0.1 ppm

97 Ammonia Nitrogen Determine if Curve and QC meet criteria set forth in SOP, as well as peaks having proper shape and area similar to historically acceptable runs. Analyze Samples, LCB, LCS and continuing QC, if data is acceptable as per SOP, report results.

98 Questions?